Application of the Log-normal Model for Long Term High Affinity Antibody/Antigen Interactions using Bio-Layer Interferometry

Wallner J, et al., 52(2):575-587, J Math Chem, 2014

Optical biosensors are commonly used to characterize biomolecular interactions and are an important tool for quality control and process development groups. In this report, the authors compared two different theoretical binding models to determine which method most accurately interprets the results of the interaction. All binding studies were performed on the Octet QK system using Streptavidin biosensors. The results suggested that for reactions of high affinity and slow kinetics, the log-normal model provided better curve fitting tools over the more commonly used simple 1:1 interaction model.

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