Antibody Recognition of the Pandemic H1N1 Influenza Virus Hemagglutinin Receptor Binding Site

Hong M, et al., 87(22), 12471-80, J Virol, 2013

This study presents the crystal structure of human monoclonal antibody 5J8 Fab in complex with a bacterially expressed and refolded globular head domain from the hemagglutinin (HA) of the H1N1 pandemic virus, and reports that 5J8 recognizes a conserved epitope in and around the receptor binding site. Octet RED system binding studies of 5J8 Fab and IgG were performed against a panel of 12 H1 strains that spanned from 1918 to 2009. Biotinylated HAs were loaded onto Streptavidin biosensors and incubated with various concentrations of Fab or IgG of 5J8 or CH65 for these experiments.

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