Antibody Mimetic Receptor Proteins for Label-Free Biosensors

Raina M, et al., 140(3):803-10, Analyst, 2015

The authors describe a detailed characterization of a model receptor protein based on a non-antibody scaffold protein (which binds to anti-myc tag antibody) as the target specific receptor molecule in a label-free electrochemical biosensor. A Pall ForteBio BLItz system was used to characterize binding interactions between the receptor proteins and the anti-myc tag antibody. HIS-tagged receptor proteins were captured using the Ni-NTA biosensor probes and different concentrations of anti-myc tag antibody solutions were used as analyte.

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