Amino Acid and Glucose Metabolism in Fed-Batch CHO Cell Culture Affects Antibody Production and Glycosylation

Fan Y, et al., 112(3):521-35, Biotechnol Bioeng, 2015

By using two different IgG-producing cell lines and two different culture media, the authors investigated the differences in cell growth, IgG expression, nutrient media consumption, sugar availability, and IgG glycosylation. This article provides the information that may help growth media development and IgG expression optimization. An Octet QK384 system equipped with Protein A biosensors was used to estimate IgG titer levels.

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