Alternative Recognition of the Conserved Stem-Epitope in Influenza A Hemagglutinin by a VH3-30-Encoded Heterosubtypic Antibody

Wyrzucki A, et al., doi: 10.1128/JVI.00178-14, J Virol, 2014

The authors isolated a monoclonal antibody designated MAB3.1 from phage display with immobilized hemagglutinin from H2N2 influenza virus used as the target. They also utilized de novo in siico-design and affinity-matured synthetic proteins to determine the antibody binding characteristics in order to gain more insight into the development of pan-influenza virus vaccines. Binding studies were performed on the Octet RED instrument and Streptavidin biosensors were used.

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