A Recombinant Clumping Factor A-containing Vaccine Induces Functional Antibodies to Staphylococcus aureus that are not Observed after Natural Exposure

Hawkins J, et al., 19(10), 1641-50, Clin Vaccine Immunol, 2012

ClfA (clumping factor A), a conserved fibrinogen (Fg) binding protein, is being investigated as a possible component of vaccine to Staphylococcus aureus. The authors have developed an assay for ClfA mediated Fg binding, that can be used to measure biologically relevant immune response in mouse and human sera. A panel of anti-ClfA monoclonal antibodies was also characterized. The Octet RED96 system and Anti-Murine IgG biosensors were used to perform epitope mapping of the mAb's against the ClfA antigen and to determine mAb-ClfA kinetic constants.

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