A Rapid Method for Determining Dynamic Binding Capacity of Resins for the Purification of Proteins

Do, T., et al., 60(2), 147-150, Protein Expression and Purification, 2008

Determining the dynamic binding capacity of resins is an essential step in optimization of a purification process. The authors describe an approach based on the use of Octet systems to compare the dynamic binding capacity of three protein A resins for a recombinant chimeric antibody. The method can be modified for quantification of any protein for which a specific capture molecule is available.

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