A Polyclonal Antibody Based Immunoassay Detects Seven Subtypes of Shiga Toxin 2 Produced by Escherichia coli in Human and Environmental Samples

He X, et al., 8(10):e76368, PLoS One, 2013

This study describes the development of a sandwich ELISA assay for detecting both O157 and non-O157 strains of Shiga toxin-producing Escherichia coli (STEC) by incorporating a novel polyclonal antibody against Shiga toxin 2 (Stx2). Real-time binding assays between purified antibodies and purified Stx2a protein were performed on the Octet QK system with biotinylated pAb coupled to Streptavidin biosensors.

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