A Novel Synthetic Small Molecule YH-306 Suppresses Colorectal Tumour Growth and Metastasis Via FAK Pathway

Dai F, et al., 19(2):383-95, J Cell Mol Med, 2015

With the intention of finding a small molecule drug candidate against colorectal cancer (CRC) metastasis, the authors performed a comprehensive study on an aryl tetrahydro-β-carboline called YH-306 using a variety of assays. The estimation of binding between YH-306 and Arp2/3 protein complex was achieved by using an Octet QK system equipped with Streptavidin Biosensor probes. Biotinylated Arp2/3 protein complex was captured on to Streptavidin probes while YH-306 or CK-636 (positive control) analytes were prepared in binding buffer (25 mM Na HEPES (pH 8.0), 50 mM arginine-glutamate and 150 mM NaCl).

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