A Novel Rabbit Monoclonal Antibody Platform To Dissect the Diverse Repertoire of Antibody Epitopes for HIV-1 Env Immunogen Design

Chen Y, et al., 87(18), 10232-43, J Virol, 2013

In this study, 12 HIV-1 Env-specific rabbit hybridoma cell lines were produced that recognized a wide range of Env epitopes. Heavy-chain and light-chain genes were cloned and their genetic features were analyzed. Binding kinetics of the rabbit mAb to gp120s from HIV-1 clades A to E were studied using the Octet QKe system. Protein A biosensors were loaded with the rabbit mAbs and incubated with concentration series of gp120 proteins.

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