A Novel Hemoglobin-binding Peptide Reduces Cell-free Hemoglobin in Murine Hemolytic Anemia

Hanson, M S, et al., 304, H328-36, Am J Physiol Heart Circ Physiol, 2013

In this study, a novel hemoglobin-binding peptide (Hb-B10) was synthesized and used to study the contribution of cell-free hemoglobin to vascular complications associated with hemolysis. The binding of the Hb-B10 peptide to hemoglobin was characterized using the Octet RED96 system. Biotinylated Hb-B10 peptide was loaded onto Streptavidin biosensors and incubated with various concentrations of hemoglobin to determine kinetic binding constants.

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