A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism

Liu Z, et al., 290(12):7535-62, J Biol Chem, 2015

The article describes a novel approach for the production of bispecific heterodimeric IgG antibody in mammalian cells. The antibody consists of two different heavy chains and two different light chains that were paired by an antibody engineering approach. The protein expression levels in harvested media were estimated using an Octet RED96 equipped with Protein A biosensors.

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