A Mutant Tat Protein Inhibits HIV-1 Reverse Transcription by Targeting the Reverse Transcription Complex

Lin M, et al., 89(9):4827-36, J Virol, 2015

This study has focused on the mechanism of reverse transcription inhibition by Nullbasic in HIV-1. Direct binding of Nullbasic to HIV-1 Reverse Transcriptase (RT) was demonstrated by a variety of techniques including the BLI. An Octet RED system equipped with SA biosensors was used to capture the biotinylated RT. Titrations carried out in the presence of Nullbasic as analyte confirmed direct binding between the two proteins. This interaction appears to be uncoating the viral core and destabilizes the viral reverse transcription complex (RTC).

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