A Multi-Pronged Investigation into the Effect of Glucose Starvation and Culture Duration on Fed-Batch CHO Cell Culture

Fan Y, et al., 112(10):2172-84, Biotechnol Bioeng, 2015

In this study, the effects of glucose starvation and culture duration on monoclonal antibody production in CHO cell cultures were investigated. The effects of these process parameters on the titer, N-glycosylation, proteomic signature, and the intrinsic properties of the cells were evaluated. Monoclonal antibody production in the cell culture was evaluated by an Octet QK384 system equipped with Protein A biosensors.

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