A High-Affinity Native Human Antibody Neutralizes Human Cytomegalovirus Infection of Diverse Cell Types

Kauvar L, et al., 59(3):1558-68, Antimicrob Agents Chemother, 2015

By using a previously reported platform known as the single-B-cell screening, the authors cloned 30 different native human MAbs that bind to site I of AD-2 epitope on viral glycoprotein B (gB). Despite a reasonably high affinity, one of the antibodies (TRL345) also exhibited the best in vitro potency allowing the authors to carry out this detailed biological and biochemical evaluation. The KD measurement were achieved by an Octet QK system equipped with Streptavidin biosensors. This has allowed them to immobilize C-terminal biotinylated AD-2 peptide (HRANETIYNTTLKYGD-biotin) and monitor binding with TRL345 in a concentration dependent manner.

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