A Diversity of Antibody Epitopes Can Induce Signaling Through the Erythropoietin Receptor

Lim, A.C., et al., 49(18), 3797-3804, Biochemistry, 2010

A panel of single chain Fc fusion constructs were generated to agonize the erythropoietin receptor (EpoR). The Octet RED platform was used to map the epitopes to which the scFcv constructs bound using cross-competition experiments. These studies used immobilize biotinylated EpoR immobilized on Streptavidin biosensors. For competition binding experiments, immobilized EpoR was saturated with an individual scFv-Fc protein, and then incubated with a second scFv-Fc protein.

Read More