A Combination of Three Fully-Human Toxin A- and Toxin B-Specific Monoclonal Antibodies Protects against Challenge with Highly Virulent Epidemic Strains of C. difficile in the Hamster Model

Anosova N, et al., 22(7):711-25, Clin Vaccine Immunol, 2015

The development of an 3-antibody cocktail (3-MAbs) against highly virulent strains of Clostridium difficile is reported. The cocktail targets C. difficile toxins A and B. Authors used a high-throughput B-cell cloning strategy to isolate monoclonal antibodies (MAbs) from healthy human donors. Affinity measurements involved a Pall ForteBio Octet RED96 system equipped with Protein A biosensors to capture MAbs. These MAb captured sensors were subsequently immersed in solutions containing toxin A or toxin B for kinetic measurements.

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