A Cell-Based Internalization and Degradation Assay with an Activatable Fluorescence-Quencher Probe as a Tool for Functional Antibody Screening

Li Y, et al., 20(7):869-75, J Biomol Screen, 2015

This article describes the development of a novel fluorescence pair to evaluate the extent of antibody internalization and degradation in target cells. An Octet system was used for antibody quantitation in supernatants. Human IgG was used as the standard.

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