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A Broadly Neutralizing Human Monoclonal Antibody that Recognizes a Conserved, Novel Epitope on the Globular Head of the Influenza H1N1 Virus Hemagglutinin

Krause, J.C., et al., 85(20), 10905-10908, Journal of Virology, 2011

The conserved influenza virus hemagglutinin (HA) stem domain elicits cross-reactive antibodies, but epitopes in the globular head typically elicit strain-specific responses because of the hypervariability of this region. The authors isolated human monoclonal antibody 5J8 that neutralized a broad spectrum of 20th century H1N1 viruses and the 2009 pandemic H1N1 virus. Detailed mapping of the interaction unexpectedly revealed a novel epitope on HA. Binding affinities of 5J8 Fab to recombinant trimeric His-tagged HA proteins were determined using Anti-Penta-HIS biosensors on an Octet RED instrument.

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