3, 4-dihydroxyl-phenyl Lactic Acid Restores NADH Dehydrogenase 1 α Subunit 10 to Ameliorate Cardiac Reperfusion Injury

Yang X, et al., 5:10739, Sci Rep, 2015

The role of 3, 4-dihydroxyl-phenyl lactic acid (DLA) in cardioprotection against ischemia/reperfusion (I/R) injury and its ability to interact and activate sirtuin1 (SIRT1) were demonstrated in this study. The activation of SIRT1 causes enhanced expression of NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 (NDUFA10) and mitochondrial function after I/R. An Octet RED instrument equipped with super Streptavidin Biosensor probes was used to capture biotinylated SIRT1 antibody followed by human recombinant SIRT1. The DLA analyte binding to SIRT1 was monitored in real time for K determination. In addition to identifying DLA mediated signaling pathway, the findings provide clues for designing new medicines for I/R induced cardiac injury.

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