Citations

ClonePix 2 System

Number of Citations*: 404

Latest Citations: For a complete list, please click here.

High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system

JJC Hou, BS Hughes, M Smede, KM Leung, K Levine… - New biotechnology, 2014 - Elsevier

Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high- expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to …

Cited by 33 Related articles All 8 versions

Rapid isolation of antigen-specific clones from hybridoma fusions

CJ Mann - Nature Methods, 2007 - nature.com

… The Genetix ClonePix FL is a powerful way to find and collect antigen-specific clones from hybridoma fusions in a rapid one-step process—shortening the process timeline and allowing parallel interrogation of multiple antigens in the monoclonal antibody discovery process …

Cited by 12 Related articles All 3 versions

Using ClonePix FL To Assess Monoclonality

JF Burke, CJ Mann, S Jiang… - GENETIC …, 2009

- MARY ANN LIEBERT INC 140 …

Cited by 2 Related articles

Sequential screening by ClonePix FL and intracellular staining facilitate isolation of high producer cell lines for monoclonal antibody manufacturing

G Roy, G Miro-Quesada, L Zhuang, T Martin… - Journal of …, 2017 - Elsevier

Screening and characterization of cell lines for stable production are critical tasks in identifying suitable recombinant cell lines for the manufacture of protein therapeutics. To aid this essential function we have developed a methodology for the selection of antibody …

Cited by 1 Related articles All 5 versions

[PDF] researchgate.net

Rapid automated selection of mammalian cell colonies by cell surface protein expression

ENC Newman, D Whitney - Nature Methods, 2007 - nature.com

… Abstract. Genetix's unique ClonePix FL technology uses our ability to grow mammalian cells into clonal colonies suspended in semisolid media, and image these colonies using fluorescence assays and markers. Here we describe …

Cited by 3 Related articles All 2 versions

Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker

S Dharshanan, CS Hung - Monoclonal Antibodies, 2014 - Springer

… 3.2 Sanitization and Calibration of ClonePix FL System. Diligently follow the instructions of the pre-programmed software in the ClonePix FL system. 1 … Sanitize the system with ultraviolet light for 10 min. 3.3 Selection of High-Producing Transfectomas Using ClonePix FL System …

Cited by 10 Related articles All 8 versions

[PDF] researchgate.net

Regulatory perspective on the evaluation of clonality of mammalian cell banks

R Novak - CASSS Strategy Forum, 2017 - casss.org

… acceptable. • Other techniques including FACS and ClonePix can provide high probability of clonality when performed using the correct procedures and parameters. • Imaging techniques could be used to supplement data from LD, FACS or ClonePix www.fda.gov Page 16. 16 …

Cited by 3 Related articles All 2 versions View as HTML

[PDF] casss.org

An improved clone selection method

U Jamnikar, M Blas, D Gaser… - Proceedings of the 21st …, 2012 - Springer

In most cases there are no resources to isolate and analyze more than few 100 clones. ClonePix FL (CP-FL, Genetix) technology substantially increases the number of screened clones in the selected pool before expansion in microtiter plates … 1.2.1 ClonePix FL …

Cited by 2 Related articles All 4 versions

Improving Clone Production for Increased Protein Yield from Mammalian Cell Lines

S Williams, R Cranenburgh - Innovations in Pharmaceutical …, 2008 - iptonline.com

… An alternative approach for the screening of secreting clones is the use of cell imaging systems such CellXpress marketed by Sigma-Aldrich Corporation, and ClonePix FL from Genetix Ltd. ClonePix FL can screen around ten thousand in three weeks …

Cited by 2 Related articles All 3 versions

[PDF] iptonline.com

Stem and iPS cell selection: quantitation of surface marker (SSEA1) and intracellular GFP

CJ Mann, ENC Newman, DJ Whitney, NJ Latchem… - Nature …, 2007 - nature.com

… The Genetix ClonePix FL technology, already established for the rapid one-step identification and isolation of hybridomas and cell lines for biopharmaceutical production, can be used to isolate and collect stem cells expressing specific cell surface and intracellular proteins …

Cited by 1 Related articles All 3 versions

Number of Citations*: 404

Latest Citations: For a complete list, please click here.

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