Perform your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader


The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on sensitivity or performance. Included SoftMax® Pro Software enables rapid data generation and analysis helping you achieve more in less time.

  • Easy-to-use


    Save time on training, data generation, and data analysis, for faster results.

  • Flexible


    Perform your favorite applications with a user-friendly reader that accommodates plate types from six to 384-well formats; up to three modes of detection measuring absorbance with NFC-tagged filters for fluorescence, luminescence, or both.

  • Affordable


    Gain access to over thirty years of experience and technology in a compact, multi-mode reader that was built for researchers with sensitive budgets.

Achieve more with the SpectraMax Mini

Achieve more with the SpectraMax Mini


  • Industry-leading software

    Industry-leading software

    Enjoy an extensive library of protocols, advanced reader control, and easy data management and analysis with our best-in-class Softmax Pro Software.

  • Expanded wavelength range

    Expanded wavelength range

    Xenon flash lamps allow you to excite samples in the UV range where halogen does not.

  • Approachable design

    Approachable design

    Effortless functionality provides users with everything they need to run their most crucial applications quickly and without complications.

  • AutoPMT technology

    AutoPMT technology

    Our AutoPMT feature allows a wide range of concentrations to be prepared and read on one plate, avoiding saturation (or the loss of signal) and saving you time and money.

Save time and money with a    
microplate reader and washer bundle

Microplate Reader and Washer Bundle

Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution:

  • The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence.
  • SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options.
  • The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options.
  • Save on a bundle

Automated Microplate Washer and Reader Solution

Latest Resources


Applications of SpectraMax Mini Multi-Mode Microplate Reader

  • Absorbance


    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    Cell Health


    Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.

    Learn more 

  • Cellular Signaling

    cellular signaling

    Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.

    Learn more 



    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, typically in a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

  • Fluorescence


    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  



    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  

  • Microbiology and Contaminant


    Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.

    Learn more 

    Nucleic Acid (DNA/RNA) Quantitation and Analysis

    Nucleic Acid

    Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.

    Learn more 

  • Protein Detection, Quantitation and Analysis

    Protein Detection

    Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.

    Learn more 

Specifications & Options of SpectraMax Mini Multi-Mode Microplate Reader

Resources of SpectraMax Mini Multi-Mode Microplate Reader

Videos & Webinars
Leverage Automated, end-to-end workflows to enable complex Organoid Assays

Leverage automated, end-to-end workflows to enable complex organoid assays

Achieve more with the SpectraMax Mini

Achieve more with the SpectraMax Mini

Small Lab, Big Challenges

Small Lab, Big Challenges - How the Right Reader can Impact Productivity