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Multi-wavelength measurement without filters

The Gemini™ XPS and EM Microplate Readers with dual monochromators provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Multiple-point well scanning optimizes cell-based assay sensitivity. Comparison of relative fluorescence units (RFUs) between samples is allowed by a unique calibration against an internal standard. Temperature-sensitive reactions are monitored with consistent temperature regulation from ambient to 45°C.

  • Eliminate changing out filters

    Eliminate changing out filters

    Eliminate the need for identifying, purchasing, and changing out filters. Dual monochromators provide excitation and emission wavelength selection between 250-850 nm.

  • Measure more accurately

    Measure more accurately

    Well scanning reports fluorescent measurements from a single point in the center of a microplate well to multiple points across a tissue culture well.

  • Avoid losing high signals

    Avoid losing high signals

    Avoid losing high signals to detector saturation and find the correct reader settings with our patented Auto PMT Optimization System.

Gemini

Gemini

Features

  • Top-read capability

    The top-reading Gemini XPS Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.

  • Top- and bottom-read capability

    The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.

Applications of Gemini XPS and EM Microplate Readers

  • ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

    Fluorescence

    Fluorescence

    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

  • Fluorescent Protein Detection

    Fluorescent Protein Detection

    Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

    Read Application Note 

    Fluorescent Protein Quantitation

    Fluorescent Protein Quantitation

    Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.

    Learn more about fluorescent protein in our application note:

Specifications & Options of Gemini XPS and EM Microplate Readers

 

*Using lowest settings and speed read when available.

Resources of Gemini XPS and EM Microplate Readers

Gemini™ XPS Microplate Reader

Product

Product Number

Gemini XPS Microplate Reader XPS
SpectraTest FL1 Fluorescence Validation Plate 0200-5060
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume HTS Kit 0200-6267
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

Gemini™ EM Microplate Reader

Product

Part Number

Gemini EM Microplate Reader EM
SpectraTest FL1 Fluorescence Validation Plate 0200-5060
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Microplate Reader Shelf 9000-0756

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