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Gemini XPS and EM Microplate Readers

Fluorescence detection without filters
Multi-wavelength measurement without filters
The Gemini™ XPS and EM Microplate Readers with dual monochromators provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Multiple-point well scanning optimizes cell-based assay sensitivity. Comparison of relative fluorescence units (RFUs) between samples is allowed by a unique calibration against an internal standard. Temperature-sensitive reactions are monitored with consistent temperature regulation from ambient to 45°C.
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Eliminate changing out filters
Eliminate the need for identifying, purchasing, and changing out filters. Dual monochromators provide excitation and emission wavelength selection between 250-850 nm.
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Measure more accurately
Well scanning reports fluorescent measurements from a single point in the center of a microplate well to multiple points across a tissue culture well.
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Avoid losing high signals
Avoid losing high signals to detector saturation and find the correct reader settings with our patented Auto PMT Optimization System.

Gemini
Features
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Top-read capability
The top-reading Gemini XPS Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.
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Top- and bottom-read capability
The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.
Latest Resources
Applications of Gemini XPS and EM Microplate Readers
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cAMP Assays (GPCR)
Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of Gi/Gs protein-coupled receptors. cAMP levels can be monitored using fluorescent molecules that bind to cAMP and are detected using a fluorescence plate reader.
Here are a few application notes on cAMP assays (GPCR) you may find of interest:
Caspase-3 Apoptosis Assays
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as embryonic development, as well as diseases including cancer and neurodegenerative conditions. Assays for apoptosis can be performed using a variety of imaging or microplate reader detection systems and provide valuable information on normal and disease-related mechanisms of cell death.
Learn about a Caspase-3 single-step, homogenous assay that is specifically designed for microplate readers:
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Cell Health
Cell viability refers to the number of healthy cells in a population and can be evaluated using assays that measure enzyme activity, cell membrane integrity, ATP production, and other indicators. These methods can employ luminescent, fluorescent, or colorimetric readouts as indicators of general cell viability or even specific cellular pathways. Cytotoxicity and cell viability assays are often used to assess a drug or other treatment’s effect, and are valuable tools in the search for new therapeutics, as well as advancing our understanding of how normal cells function.
Cell Proliferation Assays
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth.
This application note describes two assay methods of a cell prolfieration. In the first, cellular proliferation is quantitated using a cell-based standard curve. In the second, cellular proliferation is quantitated using RNase-treated cell samples and a DNA standard curve.
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Cytotoxicity Assays
Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. There has been great interest in using stem cells as tools for screening compounds during early drug development.
In this scientific poster, we present results obtained from one of our multi-mode plate readers for performing toxicity assessment of various compounds using stem cell-derived cell models in a high throughput manner:
EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation into the mechanisms of cell death to the development of new therapeutics targeting apoptosis in diseases. One of the most popular detection technologies for cell viability is a fluorescence microplate reader.
Here are a few applications you may find of interest:
- Measuring cell health on the SpectraMax iD3 reader with cell viability assays
- EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Caspase-3/7 R110 Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Glutathione Assay Kit on SpectraMax Fluorescence Microplate Readers
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ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
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Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.
Fluorescent Protein Quantitation
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Learn more about fluorescent protein in our application note:
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Microbiology and Contaminant
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s biomass, and microbes in the human body outnumber human cells by 10 to 1. These microorganisms provide great benefit to us and are also vital to many fields of research from medicine to alternative energy production. On the other hand, monitoring for microbes and the toxic substances they produce is necessary to ensure the safety of pharmaceutical products. Scientists whose research relies on mammalian cells must carefully monitor these cultures for unwanted microbial contaminants to ensure that their experimental results are reliable.
Nucleic Acid (DNA/RNA) Quantitation and Analysis
Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.
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Protein Detection, Quantitation and Analysis
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.
Tryptophan detection
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan dominates the emission of proteins and is the most sensitive to solvent polarity and the local environment. Analysis of changes in tryptophan fluorescence can yield information on protein denaturation and conformation.
In these application notes, we demonstrate performance of the SpectraMax® multi-mode microplate readers for assays measuring intrinsic tryptophan fluorescence.
Specifications & Options of Gemini XPS and EM Microplate Readers
Resources of Gemini XPS and EM Microplate Readers
Publications
Millions in FDA Fines and Thousands of Warning Letters: How GxP Compliance Software Can Help Avoid Them
Millions in FDA Fines and Thousands of Warning Letters: How GxP Compliance Software Can Help Avoid Them
The pharmaceutical world is a highly regulated environment. And for good reason. As a potential drug moves through the development process – from the in vitro discovery phase, to pre…
Blog
[Podcast] Challenges of traditional cell line development and emerging technologies to help regulate monoclonality
[Podcast] Challenges of traditional cell line development and emerging technologies to help regulate monoclonality
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
Blog
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs
The regulations for food and drug in the United States, described in the Title 21 of the Code of Federal Regulations, and the EudraLex Annex 11 in EU, are critical in ensuring safe…
Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
Customer Breakthrough
ApresLabs use SpectraMax readers to revolutionize research into pesticide resistance
ApresLabs use SpectraMax readers to revolutionize research into pesticide resistance
Resistance to insecticides is a major obstacle to controlling insect pests in a sustainable manner in the agricultural industry. Aware of this issue, Dr Graham Moores founded ApresLa…
Application Note
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
This application note describes how to use the NanoOrange® Protein Quantitation Kit from Life Technologies in SpectraMax® microplate readers with the fluorescence detection mode and…
eBook
Detect Nucleic Acids and Proteins Like a Pro
Detect Nucleic Acids and Proteins Like a Pro
Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments.
Application Note
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth. The CyQUANT Cell Proliferation…
Scientific Poster
A Stable, Sensitive Fluorescence-Based Method for Detecting Cyclic AMP
A Stable, Sensitive Fluorescence-Based Method for Detecting Cyclic AMP
Cyclic AMP is involved in intra- and inter-cellular signaling in organisms as diverse as bacteria, slime mold, fruit flies and humans (4, 5, 8, 9). Cyclic AMP is produced from ATP by…
Data Sheet
SpectraMax Gemini XPS Microplate Reader
SpectraMax Gemini XPS Microplate Reader
The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most f…
Scientific Poster
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes unnecessary proteins by breaking them down into short peptides. It consists o…
Data Sheet
SpectraMax Gemini EM Microplate Reader
SpectraMax Gemini EM Microplate Reader
Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual monochromator system.
Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. O…
Number of Citations*: 10,700
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
- Dated: May 01, 2011Publication Name: Bentham Science Publishers
Amino Acid-Substituted Gemini Surfactant-Based Nanoparticles as Safe and Versatile Gene Delivery Agents
Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have… View moreGene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have designed and developed a series of novel amino acid-substituted gemini surfactants with the general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine, glycyl-lysine and, lysyl-lysine). These compounds were synthesized and tested in rabbit epithelial cells using a model plasmid and a helper lipid. Plasmid/gemini/lipid (P/G/L) nanoparticles formulated using these novel compounds achieved higher gene expression than the nanoparticles containing the parent unsubstituted compound. In this study, we evaluated the cytotoxicity of P/G/L nanoparticles and explored the relationship between transfection efficiency/toxicity and their physicochemical characteristics (such as size, binding properties, etc.). An overall low toxicity is observed for all complexes with no significant difference among substituted and unsubstituted compounds. An interesting result revealed by the dye exclusion assay suggests a more balanced protection of the DNA by the glycine and glycyl-lysine substituted compounds. Thus, the higher transfection efficiency is attributed to the greater biocompatibility and flexibility of the amino acid/peptide-substituted gemini surfactants and demonstrates the feasibility of using amino acid-substituted gemini surfactants as gene carriers for the treatment of diseases affecting epithelial tissue.
Contributors: Singh, Jagbir; Yang, Peng; Michel, Deborah; E. Verrall, Ronald; Foldvari, Marianna; Badea, Ildiko
Go to article - Dated: Feb 24, 2010Publication Name: ASSAY and Drug Development Technologies
A Human CXCL13-Induced Actin Polymerization Assay Measured by Fluorescence Plate Reader
The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced… View moreThe chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced chemotaxis may be a fruitful approach for developing therapeutics in treating these diseases. Cells undergo cytoskeletal rearrangement prior to chemotaxis, and therefore actin polymerization can be used as a surrogate readout more proximal to chemokine receptor activation than chemotaxis. Conventionally, actin polymerization is measured by fluorescence microscopy or flow cytometry, which are either of low throughput or in need of special instruments. We developed a 96-well actin polymerization assay that can process 1,000 to 1,500 samples a day. This assay uses a standard laboratory fluorescence microplate reader as the detection instrument and was optimized for various experimental conditions such as cell density, actin filament staining reagent, staining buffer, and cell culture conditions. We demonstrate that this actin polymerization assay in 96-well format exhibits the expected pharmacology for human CXCR5 and is suitable as a primary functional assay to screen neutralizing scFv in crude bacterial peri-preps and a secondary assay for small compound collections.
Contributors: Jennifer Alley, Laird Bloom, Marion Kasaian, Huilan Gao, Gabriel Berstein, James D. Clark, and Wenyan Miao
Go to article - Dated: May 09, 2005Publication Name: Journal of Materials Chemistry
Phosphorescent oxygen-sensitive materials for biological applications
A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor… View moreA number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor cellular oxygen uptake and their ability to overcome a number of problems associated with oxygen sensing in biological samples was assessed. Macromolecular probes proved sufficient in a number of cases, particularly where spectral solutions can resolve the interferences. However where physical interactions cause interference the added protection of the polymer in the particle based probes was required.
Gemini™ XPS Microplate Reader
Product |
Product Number |
Gemini XPS Microplate Reader | XPS |
SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
SpectraDrop™ Micro-Volume HTS Kit | 0200-6267 |
StakMax Microplate Handling Stacker | StakMax |
Microplate Reader Shelf | 9000-0756 |
Gemini™ EM Microplate Reader
Product |
Part Number |
Gemini EM Microplate Reader | EM |
SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
SpectraDrop™ Micro-Volume Microplate HTS Kit | 0200-6267 |
Microplate Reader Shelf | 9000-0756 |
Related Products & Services of Gemini XPS and EM Microplate Readers
Featured Applications

cAMP Assays (GPCR)
Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate…

Caspase-3 Apoptosis Assays
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as…

Cell Health
Cell viability refers to the number of healthy cells in a population and can be evaluated using…

Cell Proliferation Assays
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of…

Cytotoxicity Assays
Development of predictive in vitro assays suitable for safety and efficacy testing is of high…

EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation…

ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…

Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to…

Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in…

Fluorescent Protein Quantitation
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between…

Microbiology and Contaminant
Microbes, including bacteria, have been estimated to make up about 15 percent of the earth’s…

Nucleic Acid (DNA/RNA) Quantitation and Analysis
Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA)…

Protein Detection, Quantitation and Analysis
Protein detection, quantitation, and analysis are central to the investigation of a wide variety of…

Tryptophan detection
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and…
Customer Breakthrough


SUCCESS STORY
ApresLabs use SpectraMax readers to revolutionize research into pesticide resistance
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…