Gemini XPS and EM Microplate Readers

ELISA

Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

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Fluorescence

Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

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Fluorescent Protein Detection

Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

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Fluorescent Protein Quantitation

Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.

Learn more about fluorescent protein in our application note:

• Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
• Using the NanoOrange Protein Kit with SpectraMax Microplate Readers