Gemini XPS and EM Microplate Readers
Fluorescence detection without filters
Multi-wavelength measurement without filters
The Gemini™ XPS and EM Microplate Readers with dual monochromators provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Multiple-point well scanning optimizes cell-based assay sensitivity. Comparison of relative fluorescence units (RFUs) between samples is allowed by a unique calibration against an internal standard. Temperature-sensitive reactions are monitored with consistent temperature regulation from ambient to 45°C.
Features
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Top-read capability
The top-reading Gemini XPS Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.
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Top- and bottom-read capability
The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes.
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Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about 250…
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Application Note
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
This application note describes how to use the NanoOrange® Protein Quantitation Kit from Life Technologies in SpectraMax® microplate readers with the fluorescence detection mode and SoftMax…
Applications of Gemini XPS and EM Microplate Readers
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cAMP Assays (GPCR)
Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of Gi/Gs protein-coupled receptors. cAMP levels can be monitored using fluorescent molecules that bind to cAMP and are detected using a fluorescence plate reader.
Here are a few application notes on cAMP assays (GPCR) you may find of interest:
cAMP Assays (GPCR)
Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of Gi/Gs protein-coupled receptors. cAMP levels can be monitored using fluorescent molecules that bind to cAMP and are detected using a fluorescence plate reader.
Here are a few application notes on cAMP assays (GPCR) you may find of interest:
Caspase-3 Apoptosis Assays
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as embryonic development, as well as diseases including cancer and neurodegenerative conditions. Assays for apoptosis can be performed using a variety of imaging or microplate reader detection systems and provide valuable information on normal and disease-related mechanisms of cell death.
Learn about a Caspase-3 single-step, homogenous assay that is specifically designed for microplate readers:
Caspase-3 Apoptosis Assays
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as embryonic development, as well as diseases including cancer and neurodegenerative conditions. Assays for apoptosis can be performed using a variety of imaging or microplate reader detection systems and provide valuable information on normal and disease-related mechanisms of cell death.
Learn about a Caspase-3 single-step, homogenous assay that is specifically designed for microplate readers:
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Cell Proliferation Assays
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth.
This application note describes two assay methods of a cell prolfieration. In the first, cellular proliferation is quantitated using a cell-based standard curve. In the second, cellular proliferation is quantitated using RNase-treated cell samples and a DNA standard curve.
Cell Proliferation Assays
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth.
This application note describes two assay methods of a cell prolfieration. In the first, cellular proliferation is quantitated using a cell-based standard curve. In the second, cellular proliferation is quantitated using RNase-treated cell samples and a DNA standard curve.
Cytotoxicity Assays
Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. There has been great interest in using stem cells as tools for screening compounds during early drug development.
In this scientific poster, we present results obtained from one of our multi-mode plate readers for performing toxicity assessment of various compounds using stem cell-derived cell models in a high throughput manner:
Cytotoxicity Assays
Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. There has been great interest in using stem cells as tools for screening compounds during early drug development.
In this scientific poster, we present results obtained from one of our multi-mode plate readers for performing toxicity assessment of various compounds using stem cell-derived cell models in a high throughput manner:
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EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation into the mechanisms of cell death to the development of new therapeutics targeting apoptosis in diseases.
One of the most popular detection technologies for cell viability is a fluorescence microplate reader. Here are a few applications you may find of interest:
- Measuring cell health on the SpectraMax iD3 reader with cell viability assays
- EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Caspase-3/7 R110 Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Glutathione Assay Kit on SpectraMax Fluorescence Microplate Readers
EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation into the mechanisms of cell death to the development of new therapeutics targeting apoptosis in diseases.
One of the most popular detection technologies for cell viability is a fluorescence microplate reader. Here are a few applications you may find of interest:
- Measuring cell health on the SpectraMax iD3 reader with cell viability assays
- EarlyTox Live/Dead Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Caspase-3/7 R110 Assay Kit on SpectraMax Fluorescence Microplate Readers
- EarlyTox Glutathione Assay Kit on SpectraMax Fluorescence Microplate Readers
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.
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Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.
Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.
Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.
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Fluorescent Protein Quantitation
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Learn more about fluorescent protein in our application note:
Fluorescent Protein Quantitation
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Learn more about fluorescent protein in our application note:
Nucleic Acid Quantitation
Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-generation sequencing. Compared to spectrophotometric DNA quantification, the fluorometric method provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules).
Here are a few applications notes on nucleic acid quantitation you may find of interest:
Nucleic Acid Quantitation
Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-generation sequencing. Compared to spectrophotometric DNA quantification, the fluorometric method provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules).
Here are a few applications notes on nucleic acid quantitation you may find of interest:
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Tryptophan Detection
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the most sensitive to solvent polarity and the local environment.
In these application notes we demonstrate performance of the SpectraMax multi-mode microplate readers for assays measuring intrinsic tryptophan fluorescence.
Tryptophan Detection
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the most sensitive to solvent polarity and the local environment.
In these application notes we demonstrate performance of the SpectraMax multi-mode microplate readers for assays measuring intrinsic tryptophan fluorescence.
Specifications & Options of Gemini XPS and EM Microplate Readers
Resources of Gemini XPS and EM Microplate Readers
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Application Note
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However, this method is only able to measure down to about…
-
Application Note
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
Using the NanoOrange Protein Kit with SpectraMax Microplate Readers
This application note describes how to use the NanoOrange® Protein Quantitation Kit from Life Technologies in SpectraMax® microplate readers with the fluorescence detection mode and…
eBook
Detect Nucleic Acids and Proteins Like a Pro
Detect Nucleic Acids and Proteins Like a Pro
Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments.
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Application Note
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth. The CyQUANT Cell Proliferation…
Scientific Poster
A Stable, Sensitive Fluorescence-Based Method for Detecting Cyclic AMP
A Stable, Sensitive Fluorescence-Based Method for Detecting Cyclic AMP
Cyclic AMP is involved in intra- and inter-cellular signaling in organisms as diverse as bacteria, slime mold, fruit flies and humans (4, 5, 8, 9). Cyclic AMP is produced from ATP by…
Data Sheet
SpectraMax Gemini XPS Microplate Reader
SpectraMax Gemini XPS Microplate Reader
The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most f…
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Scientific Poster
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
Measurement of Proteosome Inhibition in Live Cells on the Analyst® GT, FLEXstation® and Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor
The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes unnecessary proteins by breaking them down into short peptides. It consists o…
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Data Sheet
SpectraMax Gemini EM Microplate Reader
SpectraMax Gemini EM Microplate Reader
Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual monochromator system.
Brochure
Innovative Solutions for Drug Discovery and Life Sciences Research
Innovative Solutions for Drug Discovery and Life Sciences Research
Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. O…
Number of Citations*: 9,610
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Dated: May 01, 2011Publication Name: Bentham Science PublishersAmino Acid-Substituted Gemini Surfactant-Based Nanoparticles as Safe and Versatile Gene Delivery Agents
Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have… View moreGene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Recently, we have designed and developed a series of novel amino acid-substituted gemini surfactants with the general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine, glycyl-lysine and, lysyl-lysine). These compounds were synthesized and tested in rabbit epithelial cells using a model plasmid and a helper lipid. Plasmid/gemini/lipid (P/G/L) nanoparticles formulated using these novel compounds achieved higher gene expression than the nanoparticles containing the parent unsubstituted compound. In this study, we evaluated the cytotoxicity of P/G/L nanoparticles and explored the relationship between transfection efficiency/toxicity and their physicochemical characteristics (such as size, binding properties, etc.). An overall low toxicity is observed for all complexes with no significant difference among substituted and unsubstituted compounds. An interesting result revealed by the dye exclusion assay suggests a more balanced protection of the DNA by the glycine and glycyl-lysine substituted compounds. Thus, the higher transfection efficiency is attributed to the greater biocompatibility and flexibility of the amino acid/peptide-substituted gemini surfactants and demonstrates the feasibility of using amino acid-substituted gemini surfactants as gene carriers for the treatment of diseases affecting epithelial tissue.
Contributors: Singh, Jagbir; Yang, Peng; Michel, Deborah; E. Verrall, Ronald; Foldvari, Marianna; Badea, Ildiko
Go to article-
Dated: Feb 24, 2010Publication Name: ASSAY and Drug Development Technologies
A Human CXCL13-Induced Actin Polymerization Assay Measured by Fluorescence Plate Reader
The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced… View moreThe chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. Therefore, disrupting CXCL13-induced chemotaxis may be a fruitful approach for developing therapeutics in treating these diseases. Cells undergo cytoskeletal rearrangement prior to chemotaxis, and therefore actin polymerization can be used as a surrogate readout more proximal to chemokine receptor activation than chemotaxis. Conventionally, actin polymerization is measured by fluorescence microscopy or flow cytometry, which are either of low throughput or in need of special instruments. We developed a 96-well actin polymerization assay that can process 1,000 to 1,500 samples a day. This assay uses a standard laboratory fluorescence microplate reader as the detection instrument and was optimized for various experimental conditions such as cell density, actin filament staining reagent, staining buffer, and cell culture conditions. We demonstrate that this actin polymerization assay in 96-well format exhibits the expected pharmacology for human CXCR5 and is suitable as a primary functional assay to screen neutralizing scFv in crude bacterial peri-preps and a secondary assay for small compound collections.
Contributors: Jennifer Alley, Laird Bloom, Marion Kasaian, Huilan Gao, Gabriel Berstein, James D. Clark, and Wenyan Miao
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Dated: May 09, 2005Publication Name: Journal of Materials Chemistry
Phosphorescent oxygen-sensitive materials for biological applications
A number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor… View moreA number of macromolecular probes employing different carriers and a number of microparticular probes employing different oxygen sensitive dyes were fabricated, giving a panel of oxygen sensitive probes. The photophysical and oxygen sensing properties of these probes were examined comparatively. The probes were used successfully to monitor cellular oxygen uptake and their ability to overcome a number of problems associated with oxygen sensing in biological samples was assessed. Macromolecular probes proved sufficient in a number of cases, particularly where spectral solutions can resolve the interferences. However where physical interactions cause interference the added protection of the polymer in the particle based probes was required.
Gemini™ XPS Microplate Reader
|
Product |
Product Number |
| Gemini XPS Microplate Reader | XPS |
| SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
| SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
| SpectraDrop™ Micro-Volume HTS Kit | 0200-6267 |
| StakMax Microplate Handling Stacker | StakMax |
| Microplate Reader Shelf | 9000-0756 |
Gemini™ EM Microplate Reader
|
Product |
Part Number |
| Gemini EM Microplate Reader | EM |
| SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
| SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
| SpectraDrop™ Micro-Volume Microplate HTS Kit | 0200-6267 |
| Microplate Reader Shelf | 9000-0756 |
Related Products & Services of Gemini XPS and EM Microplate Readers
Featured Applications
cAMP Assays (GPCR)
Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate…
Caspase-3 Apoptosis Assays
Apoptosis is a highly regulated cellular program that causes cell death in normal processes such as…
Cell Proliferation Assays
Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of…
Cytotoxicity Assays
Development of predictive in vitro assays suitable for safety and efficacy testing is of high…
EarlyTox Cell Viability Assay Kits
Cell viability assays are critical to a broad spectrum of research areas ranging from investigation…
ELISA
Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…
Fluorescence
Learn all about fluorescence detection – what it is, how it works, and the instruments used to…
Fluorescent Protein Detection
Fluorescent proteins have become enormously popular as tools for monitoring biological events in…
Fluorescent Protein Quantitation
Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between…
Nucleic Acid Quantitation
Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and…
Tryptophan Detection
The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and…
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