Faster, high-value cloning with CloneMedia CHO Growth A
The CHO Growth A media portfolio provides a fast, simple and comprehensive solution for CHO cell-line development. When combined with CloneDetect detection agent, CloneSelect™ Imager, and ClonePix™ 2 System, they enable researchers to more efficiently develop new protein producing CHO cells lines, thus accelerating time-to-market.
- Optimized for high recombinant protein production
- Chemically defined and free of animal components
- Enhanced optical quality for colony imaging
- Fast screening and selection of high-value clones
CHO media and system solutions in cell-line development workflow
Limiting dilution or fluorescence-activated cell sorting (FACS) is traditionally used to clone CHO cells. These methods can be inefficient as they may require additional rounds of sub-cloning to obtain a high probability of monoclonality. The CloneMedia CHO Growth A is a viscous semi-solid media that immobilizes single CHO cells, allowing them to grow and form distinct, clonal colonies resulting in the reduction or elimination of additional sub-cloning steps.
XP Media CHO Growth A, CloneMedia CHO Growth A, and CloneDetect together with industry-proven ClonePix 2 System and CloneSelect Imager technologies improve efficiency of isolating high-value clones faster.
Optimized for greater antibody production
The colonies picked by the ClonePix 2 System can be grown in cell culture plates containing XP Media CHO Growth A with L-Gln. By using Molecular Devices comprehensive CHO media products, CHO cells cultured in CloneMedia CHO Growth A and XP Media CHO Growth A have superior antibody productivity compared to the CHO media offerings offered by another vendor.
Colony antibody productivity was determined by performing ELISA on clone supernatants 10 days after picking. Values are normalized to 100% confluence. Molecular Devices media products are compared to another vendor’s products.