CloneSelect Single-Cell Printer Series

Cell Line Development

Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

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Monoclonality Assurance

Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

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Single-Cell Genomics

The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.

Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.

This provides optimal basis for downstream single-cell genomic analysis.

Single-Cell Sorting

Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability, but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of single cells in a manner that maximizes cell viability, as well as provides direct evidence of clonality via a series of five images captured for each dispense of a single cell.