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CloneSelect Single-Cell Printer Series

Microfluidics-based system for single-cell cloning using image-based cell sorting
*Only available in North America, Mainland China, Hong Kong, Macau and Taiwan
Eyewitness proof of monoclonality
The CloneSelect® Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge. Sort cells using high-res imaging in either bright field or optional fluorescence and capture 5 images per each single cell deposit. Prove monoclonality, improve your efficiency, maintain and enrich your viability, and prevent cross-contamination.
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Cell isolation proof captured
Document a sequence of five images as the cell is dispensed, providing direct image evidence of monoclonality in 96- or 384-well plate formats.
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Clonal outgrowth improved
Achieve up to 8X improvement in clonal outgrowth efficiency over traditional methods. Deposit single cells with high efficiency into 96- or 384-well plates. It takes as little as ~5 min to dispense into a 96-well plate.
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Cells stay healthy and clean
Maintain cell viability with gentle sorting as seen with outgrowth assays, and prevent cross contamination with sterile, disposable one-way flow cartridge.
CloneSelect Single-Cell Printer
Features
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Image-based proof
Five images are captured each time a single-cell is dispensed, providing direct, image-based evidence of monoclonality that can be used in regulatory fillings.
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Fluorescence imaging and sorting
Sort viable cells based on live-cell fluorescent dye, enrich cell populations for high producers with fluorescent reporter, or isolate desired fluorescent CRISPR clones.
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Microfluidics technology
A patented, silicon microfluidic chip within the sterile cartridge generates pico-liter droplets that encapsulate cells. Only high-value droplets are seeded into plates.
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Label-free imaging and sorting
Sort cells based on size and morphology from a heterogenous cell population and select to deposit from 1 up to 100 cells per well.
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Dispensing a wide variety of cell types
A wide range of cell types such as CHO, HEK293, L929, t-cells and b-cells can be dispensed. Cells can range in size from 5 and 40 um.
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Sterile Cartridge
Individually-wrapped, sterile cartridges were designed so that cell samples never contact the instrument. A single cartridge can be used to print several microplates.
Applications of CloneSelect Single-Cell Printer Series
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Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
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Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Single-Cell Genomics
The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.
Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.
This provides optimal basis for downstream single-cell genomic analysis.
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Single-Cell Sorting
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of cells in a manner that maximizes cell viability, as well as providing direct evidence of clonality via a series of five images captured as cells dispense.
- A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
- A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
- Data Sheet: CloneSelect Single-Cell Printer
Specifications & Options of CloneSelect Single-Cell Printer Series
** Can address a cell culture 96-well plate with a suspension of 10 μm beads in sterile filtered water (concentration about 5x105 to 1x106 beads/ml) in <5 min in brightfield and <10 min in fluorescent sorting (f.sight only) by using optimal parameters for fast sorting by the user.
Resources of CloneSelect Single-Cell Printer Series
Publications
What an innovation centre means for 3D biology
What an innovation centre means for 3D biology
In April 2021, we launched our Organoid Innovation Centre (OIC), an initiative designed to help scientists leverage the potential of 3D biology throughout the drug discovery process.…
Blog
[Podcast] Challenges of traditional cell line development and emerging technologies to help regulate monoclonality
[Podcast] Challenges of traditional cell line development and emerging technologies to help regulate monoclonality
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
eBook
How to Implement Colony Picking Workflows with Automation
How to Implement Colony Picking Workflows with Automation
Colony picking represents a powerful tool within the workflow of mammalian cell line development and the study of microbiology.
Blog
The role of Monoclonal Antibodies against COVID-19
The role of Monoclonal Antibodies against COVID-19
Discover why mAbs are key in the fight against SARS-CoV-2 and how the pandemic has shaped their discovery and development pipeline. Over the past three years, the development of…
Blog
COVID-19 Timeline: Diagnostics, Vaccines, and Therapeutic Antibody Development
COVID-19 Timeline: Diagnostics, Vaccines, and Therapeutic Antibody Development
Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19. Join us as we explore a scientific timeline of key…
Blog
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
How to Develop Monoclonal Cell Lines for Stem Cell Engineering
Monoclonal assurance is undeniably critical in cell line development for generating therapeutics, such as monoclonal antibodies or cell-based therapies. To utilize a cell line for…
Blog
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
Accelerated Production of Monoclonal Antibodies for the Treatment of SARS-CoV-2
With solutions from Molecular Devices, scientists can fast-track the FDA approval process and accelerate monoclonal antibody discovery The timeline to identify and develop…
Brochure
Clone screening solutions
Clone screening solutions
Advance your microbial and mammalian clone screening with proven, automated technologies
Application Note
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
Blog
The evolution of CHO cells’ role in cell line development
The evolution of CHO cells’ role in cell line development
Since the first approval of recombinant insulin and human growth hormone in the early 1980s, a multitude of recombinant protein therapeutics have been approved by regulatory…
Flyer
High assurance monoclonality for cell line development
High assurance monoclonality for cell line development
Double confirmation of clonality. Image based evidence confirming single cell deposition event, and single cell in 96- and 384-well plate post sort
Blog
Get to know our Field Applications Scientist: Dwayne Carter
Get to know our Field Applications Scientist: Dwayne Carter
Dwayne Carter gives us a taste of 3D bioprinting, clone screening, and Caribbean cuisine Dwayne Carter is a cell biologist and educator who joined Molecular Devices in November 2020…
Blog
Life sciences technology predictions for 2021
Life sciences technology predictions for 2021
For over 30 years, Molecular Devices has been at the forefront of technological advances which have contributed to significant scientific breakthroughs. To kick off the new year, we…
eBook
Accelerate vaccine development
Accelerate vaccine development
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
eBook
Optimize your high-value cell lines
Optimize your high-value cell lines
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
Scientific Poster
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is deri…
Application Note
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is…
Application Note
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
Scientific Poster
A microfluidics-based single cell isolation workflow optimized for efficiency, viability, and assurance of monoclonality
A microfluidics-based single cell isolation workflow optimized for efficiency, viability, and assurance of monoclonality
Cell line development is a crucial step in upstream processes for generating biopharmaceutical molecules like monoclonal antibodies. Traditional workflows in cell line development ha…
Application Note
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
eBook
Tips from the cell line development front
Tips from the cell line development front
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Data Sheet
CloneSelect Single-Cell Printer Series
CloneSelect Single-Cell Printer Series
The CloneSelect™ Single-Cell Printer™ Series deposit single cells gently and with high efficiency using a patented, inkjet-like disposable, oneway dispensing cartridge.
Brochure
Reagents and Supplies
Reagents and Supplies
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…


Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow - AstraZeneca

Monoclonality Workflow

Stable Cell Line Development Workflow

Immunology and Vaccine Development Workflow

The many and varied workflows of cell line development

f.Sight vs. FACS and LD overview

Optimized workflow for rapid identification of neutralizing antibodies against viral particles
CloneSelect Single-Cell Printer

A Microfluidics-Based Single Cell Isolation Workflow Optimized for Efficiency, Viability, and Assurance of Monoclonality
Number of Citations*: 7
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
- Dated: Jun 28, 2018Publication Name: Genetic Engineering & Biotechnology News
Single-Cell Cloning Remains a Challenge
Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice. View moreRegulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice.
- Dated: Oct 28, 2017Publication Name: Genetic Engineering & Biotechnology News
Single-Cell Printing for Establishing Monoclonality
The increasing adoption of large protein biologics such as monoclonal antibodies (mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company. View moreThe increasing adoption of large protein biologics such as monoclonal antibodies (mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company.
CloneSelect Single-Cell Printer Series
Available from Molecular Devices in North America, China, Hong Kong, Macau and Taiwan. All other countries, please contact Cytena directly info@cytena.com
Product |
Part Number |
CloneSelect Single-Cell Printer f.sight Instrument |
CLONESELECT-F.SIGHT |
CloneSelect Single-Cell Printer c.sight Instrument |
CLONESELECT-C.SIGHT |
CloneSelect Single-Cell Printer SCP Instrument |
CLONESELECT-SCP |
Related Products & Services of CloneSelect Single-Cell Printer Series
Featured Applications

Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules,…

Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single…

Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of…
Single-Cell Genomics
The isolation of single cells remains a challenging task in single-cell genomics. Current methods…

Single-Cell Sorting
Cell line development requires the discovery of single cell-derived clones that produce high and…
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…