CloneSelect Single-Cell Printer Series
Microfluidics-based system for single-cell cloning using image-based cell sorting
*Only available in North America, Mainland China, Hong Kong, Macau and Taiwan
Eyewitness proof of monoclonality
The CloneSelect™ Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge. Sort cells using high-res imaging in either bright field or optional fluorescence and capture 5 images per each single cell deposit. Prove monoclonality, improve your efficiency, maintain and enrich your viability, and prevent cross-contamination.
Features
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Image-based proof
Five images are captured each time a single-cell is dispensed, providing direct, image-based evidence of monoclonality that can be used in regulatory fillings.
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Fluorescence imaging and sorting
Sort viable cells based on live-cell fluorescent dye, enrich cell populations for high producers with fluorescent reporter, or isolate desired fluorescent CRISPR clones.
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Microfluidics technology
A patented, silicon microfluidic chip within the sterile cartridge generates pico-liter droplets that encapsulate cells. Only high-value droplets are seeded into plates.
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Label-free imaging and sorting
Sort cells based on size and morphology from a heterogenous cell population and select to deposit from 1 up to 100 cells per well.
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Dispensing a wide variety of cell types
A wide range of cell types such as CHO, HEK293, L929, t-cells and b-cells can be dispensed. Cells can range in size from 5 and 40 um.
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Sterile Cartridge
Individually-wrapped, sterile cartridges were designed so that cell samples never contact the instrument. A single cartridge can be used to print several microplates.
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Application Note
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
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eBook
Tips from the cell line development front
Tips from the cell line development front
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Applications of CloneSelect Single-Cell Printer Series
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Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
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Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Single-Cell Genomics
The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.
Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.
This provides optimal basis for downstream single-cell genomic analysis.
Single-Cell Genomics
The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.
Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.
This provides optimal basis for downstream single-cell genomic analysis.
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Single-Cell Sorting
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of cells in a manner that maximizes cell viability, as well as providing direct evidence of clonality via a series of five images captured as cells dispense.
- A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
- A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
- Data Sheet: CloneSelect Single-Cell Printer
Single-Cell Sorting
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of cells in a manner that maximizes cell viability, as well as providing direct evidence of clonality via a series of five images captured as cells dispense.
- A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
- A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
- Data Sheet: CloneSelect Single-Cell Printer
Specifications & Options of CloneSelect Single-Cell Printer Series
** Can address a cell culture 96-well plate with a suspension of 10 μm beads in sterile filtered water (concentration about 5x105 to 1x106 beads/ml) in <5 min in brightfield and <10 min in fluorescent sorting (f.sight only) by using optimal parameters for fast sorting by the user.
Resources of CloneSelect Single-Cell Printer Series
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Application Note
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
A microfluidics-based, single cell printing and microplate imaging workflow optimized for efficiency, viability and monoclonality report generation
In this app note, we demonstrate a unified workflow that combines single cell deposition and clonality verification. We couple the use of the SCP and CSI to deposit single cells into a…
-
eBook
Tips from the cell line development front
Tips from the cell line development front
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Data Sheet
CloneSelect Single-Cell Printer Series
CloneSelect Single-Cell Printer Series
The CloneSelect™ Single-Cell Printer™ Series deposit single cells gently and with high efficiency using a patented, inkjet-like disposable, oneway dispensing cartridge.
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Application Note
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
Accelerated clone selection for recombinant CHO cells using CloneSelect Single-Cell Printer f. sight fluorescence-aided sorting
The market for biotherapeutics and therapeutic proteins was valued at $93.14 billion in 2018 and is expected to nearly double by the end of 2022.
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eBook
Accelerate vaccine development
Accelerate vaccine development
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
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eBook
Optimize your high-value cell lines
Optimize your high-value cell lines
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
Scientific Poster
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
A comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for mAb production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is deri…
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Application Note
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production
As regulations for cell line development become increasingly more stringent, researchers will be required to perform single-cell cloning and provide evidence that a cell line is…
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Scientific Poster
A microfluidics-based single cell isolation workflow optimized for efficiency, viability, and assurance of monoclonality
A microfluidics-based single cell isolation workflow optimized for efficiency, viability, and assurance of monoclonality
Cell line development is a crucial step in upstream processes for generating biopharmaceutical molecules like monoclonal antibodies. Traditional workflows in cell line development ha…
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Application Note
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
A workflow combining single-cell isolation and microplate imaging improves cloning efficiency with higher assurance of clonality
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
Brochure
Reagents and Supplies
Reagents and Supplies
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…
Stable Cell Line Development Workflow
Immunology and Vaccine Development Workflow
The many and varied workflows of cell line development
f.Sight vs. FACS and LD overview
Optimized workflow for rapid identification of neutralizing antibodies against viral particles
CloneSelect Single-Cell Printer
A Microfluidics-Based Single Cell Isolation Workflow Optimized for Efficiency, Viability, and Assurance of Monoclonality
Number of Citations*: 5
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Dated: Nov 19, 2019Publication Name: BiotechnologyAssuring Clonality on the Beacon Digital Cell Line Development Platform
During biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from… View moreDuring biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from Berkeley Lights offers a strategy to eliminate these limitations through culturing, manipulating, and characterizing cells on custom nanofluidic chips via software‐controlled operations. However, explicit demonstration of this technology to provide high assurance of a single cell progenitor has not been reported. Here, a methodology that utilizes the Beacon instrument to ensure high levels of clonality is described. It is demonstrated that the Beacon platform can efficiently generate production cell lines with a superior clonality data package, detailed tracking, and minimal resources. A stringent in‐process quality control strategy is established to enable rapid verification of clonal origin, and the workflow is validated using representative Chinese hamster ovary‐derived cell lines stably expressing either green or red fluorescence protein. Under these conditions, a >99% assurance of clonal origin is achieved, which is comparable to existing imaging‐coupled fluorescence‐activated cell sorting seeding methods.
Contributors: Kim Le, Christopher Tan, Huong Le, Jasmine Tat, Ewelina Zasadzinska, Jonathan Diep, Ryan Zastrow, Chun Chen, Jennitte Stevens
Go to article-
Dated: Jun 28, 2018Publication Name: Genetic Engineering & Biotechnology News
Single-Cell Cloning Remains a Challenge
Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice. View moreRegulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice.
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Dated: Oct 28, 2017Publication Name: Genetic Engineering & Biotechnology News
Single-Cell Printing for Establishing Monoclonality
The increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company. View moreThe increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company.
CloneSelect Single-Cell Printer Series
Available from Molecular Devices in North America, China, Hong Kong, Macau and Taiwan. All other countries, please contact Cytena directly info@cytena.com
| Product | Part Number |
| CloneSelect Single-Cell Printer f.sight Instrument | CLONESELECT-F.SIGHT |
| CloneSelect Single-Cell Printer c.sight Instrument | CLONESELECT-C.SIGHT |
| CloneSelect Single-Cell Printer SCP Instrument | CLONESELECT-SCP |
Related Products & Services of CloneSelect Single-Cell Printer Series
Featured Applications
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules,…
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single…
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of…
Single-Cell Genomics
The isolation of single cells remains a challenging task in single-cell genomics. Current methods…
Single-Cell Sorting
Cell line development requires the discovery of single cell-derived clones that produce high and…
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