Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
The Monoclonality Report feature streamlines the creation of supporting documentation for regulatory agencies. Reports are automatically generated based on parameters you select. The Monoclonality Report is an audit-ready document that supports filing for an Investigational New Drug (IND) Application with the FDA. (21 CFR Part 312)
Algorithms are optimized for accurate cell detection and address varying cell types and conditions. Publication-ready high-resolution imaging provides automatic confluence analysis and monoclonality assurance.
The imager delivers industry leading acquisition times, imaging a 96-well plate in as little as two minutes and can verify monoclonality from day zero with fluorescence.
Small footprint, high speed, powerful, imaging system for monoclonality verification
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green).
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing.
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types.
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training.
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days.
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
*Price, time to deliver and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
Track and view growth of every cell line
Electronically track and store plate data: cell confluence, cell number estimation, and growth curve
After initial seeding, CloneSelect Imager system can image every well, at any time point, using a ‘loci of growth’ functionality to highlight those wells that contain a single colony.
With a few simple clicks, the Monoclonality Report feature on the CloneSelect™ Imager (CSI) objectively organizes the supporting image evidence needed to establish clonality into an easily shareable report, saving researchers hours typically required to do the same process manually.
Quickly determine clonality of a cell line by visually inspecting the presence of multiple colonies in a single well. For example, the well on the left shows one colony while the cell on the right shows two.
To characterize the growth from a single cell to a colony, cell regions can be designated and adjusted for each time point in a series. The image bellow shows a single cell on Day 0 and two cells on Day 1 confirm monoclonality.
Export an entire well into 81 separate images to objectively confirm the absence of another cell. Below, a selected region is displayed over time.
Selectively highlight parts of a well to differentiate cells from ambiguous objects. A selected artifact region and its corresponding images over time is shown here.
Gene editing allows us to manipulate DNA through deletions, additions, or other modifications using several tools. There are numerous efficient strategies to carry out…
There is an unmet need in the industry for a device that allows the fast and efficient isolation of single cells while preserving their integrity and providing insurance…
Assessing monoclonality is key to the establishment of a cell line, and evidence of monoclonality is required by regulatory agencies to get a biopharmaceutical drug to…
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.
The soft agar colony formation assay is a classical and renowned technique for characterizing the ability of cells to undergo anchorage-independent growth, a hallmark of carcinogenesis. After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically incubated with compounds that may affect colony growth. The CloneSelect Imager can image every well and automatically calculate confluence, estimate colony number and area, and track colony growth.
The drug discovery landscape is shifting, with more scientists centering cell line development, disease models, and high-throughput screening methods around physiologically-relevant 3D cell models. The reason for this is clear: Using cellular model systems in research that closely mimic patient disease states or human organs can bring life-saving therapeutics to market – faster.
Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration.
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
API suite available for robotic integration. Please contact us for details
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Our solutions for antibody discovery and cell line development provide dedicated, scalable, and easy-to-use products for establishing clonal populations. The systems feature a selection…
There is an unmet need in the industry for a device that allows the fast and efficient isolation of single cells while preserving their integrity and providing insurance for their clona…
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Assessing monoclonality is key to the establishment of a cell line, and evidence of monoclonality is required by regulatory agencies to get a biopharmaceutical drug to the marketplac…
Advancements in genetic engineering and synthetic biology have allowed numerous breakthroughs in recent decades. The importance of cell line development needs an honorable mention.…
Colony picking represents a powerful tool within the workflow of mammalian cell line development and the study of microbiology.
Gene editing technology allows the manipulations at the DNA level where it is deleted, added, or modified using various tools.
Discover why mAbs are key in the fight against SARS-CoV-2 and how the pandemic has shaped their discovery and development pipeline. Over the past three years, the development of…
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Advance your microbial and mammalian clone screening with proven, automated technologies
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Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
During the last decade, production of monoclonal antibodies (mAbs) and recombinant proteins using mammalian cells has led to a boom in the introduction of biotherapeutic proteins to…
The isolation of single cells has a broad spectrum of applications, from single-cell genomics to antibody discovery and cell line development.
A variety of label-free imaging applications using the CloneSelect Imager, such as colony formation assays and clonality assessment, are presented.
Download the eBook, which features articles on monoclonality assurance, screening of stable cell lines, and tips on improving cell line productivity.
Cell line development requires screening tens of thousands of clones in order to find the few clones that are stable, grow well, and produce high yields of bioproduct. In addition to…
The development of cell lines that express a specified protein of interest is critical to the generation of biopharmaceutical molecules such as monoclonal antibodies. To establish a…
Download PDF and explore more about high throughput mammalian expression platforms & challenges with mammalian systems.
In this poster, we present data demonstrating that CloneMedia-PER.C6 supports the growth of cells into discrete colonies that can be imaged, ranked and picked using the ClonePix plat…
This PDF file explores ClonePix FL Technology which is based on the culture of mammalian cell lines in semisolid media originating from one single parent cell.
Advances in cell culture techniques are facilitating development of novel screening assays which enable drug discovery programmes to rely on cell-based assays for high-throughput scr…
Cell migration is a key process in normal development, immune function and wound healing. Abnormal cell migration is a feature of conditions such as cardiovascular disease and cancer.…
Animal cell culture technology has been thrust to the forefront of the biopharmaceutical industry because of the superior fidelity of mammalian cells in producing clinically relevant…
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…
Explore a simple one-step hybridoma selective secretion assay for detecting and isolating antigen-specific, IgG-secreting hybridoma clones in as little as 8 days from fusion with min…
Download this PDF to learn about fast track cell Line development process with integrated automated colony picker.
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Providing image evidence of monoclonality in the cell line development process is not as simple as exporting an image of a single cell. For example, high-resolution images of the ent…
Download PDF to explore the process for developing serum-free CHO cell line.
Explore non-invasive method for mouse Embrionic Stem Cell culture colony number & growth rate monitoring and identification using specific probes.
Explore CloneSelect Imager’s Migration that provides a simple, but quantitative & accurate measure of cell migration based on objective, sensitive label-free cell detection.
Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA.
The ability to monitor cell proliferation has become increasingly important, particularly with the widespread use of in vitro cell lines as model systems. Proliferation rates can be…
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Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
Monoclonality Assurance using Calcein-AM Viability Dye
Small footprint, high speed, powerful, imaging system for monoclonality verification
Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow - AstraZeneca
Stable Cell Line Development Workflow
The many and varied workflows of cell line development
Optimized workflow for rapid identification of neutralizing antibodies against viral particles
Identification and Selection of GPCR Cell Lines with ClonePix 2
Latest Citations: For a complete list, please click here .
A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.
Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.
High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported , which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.