Cell sorting with imaging and analysis for assurance of monoclonality
The CloneSelect™ Single-Cell Printer™ (SCP) utilizes microfluidics technology and real-time image analysis to sort and deposit single cells into standard microplates. An innovative cartridge, similar to ink-jet printing technology, is used for one-way dispensing. It can print a 96-well plate in 5 to 10 minutes and works with 384-well plates. Five images are captured as cells dispense, providing proof of single-cell isolation.
Sort single cells with efficiency
Sort single cells with higher efficiency (>80%) than limiting dilution and with higher viability than flow sorting (average between 50 – 90% depending on cell conditions).
Capture proof of cell isolation
Capture a sequence of five images as the cell is dispensed, providing image evidence of monoclonality. Wide depth-of-field and 10x magnification ensures image focus 99% of the time.
Differentiate single cells easily
Differentiate quickly between single cells, multi-cell, no cells, and debris with high-resolution imaging with proprietary algorithms. Single cells are plated while unwanted samples are siphoned away.
An innovative silicon microfluidic chip within the cartridge generates free-flying, pico-liter droplets that encapsulate cells. Only high-value cells are seeded into plates.
Five images are captured as single-cells are dispensed, providing image-based evidence of monoclonality that can be used in regulatory filings.
Depositing multiple cells per well
The setting changes in the software allows for dispensing 1 to 100 cells per well.
Cartridges are individually-wrapped in sterile packing to avoid cross contamination. A single cartridge can be used to print several microplates and can be refilled.
Dispensing a wide variety of cell types
A wide range of cell types such as CHO, HEK293, and L929 can be dispensed. Cells can range in size from 5 and 40 um.
The CloneSelect SCP printer features a small footprint and easily fits on lab benches, PCR workstations, as well as inside standard biosafety cabinets.
The CloneSelect Single-Cell Printer can isolate and deposit single cells into microplates while simultaneously images the entire process, thus providing image-based evidence for monoclonalit…
Applications of CloneSelect Single-Cell Printer
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability, but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of cells in a manner that maximizes cell viability, as well as providing direct evidence of clonality via a series of five images captured as cells dispense.
Specifications & Options of CloneSelect Single-Cell Printer
**Print time can vary based on cell density and gating parameters used. The average print time for beads at 1x106 beads/mL concentration was 5 - 10 minutes.
***Cell viability can vary based on cell type, sample prep, gating parameters, cell media, and other external factors. A typical viability of 75% was observed when using CHO, HEK, and other common cell lines.
Resources of CloneSelect Single-Cell Printer
The CloneSelect Single-Cell Printer can isolate and deposit single cells into microplates while simultaneously images the entire process, thus providing image-based evidence for monoclo…
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Cell Line Development
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