Overview of XP Media and CloneMedia for Mouse Hybridoma Generation

The XP Media and CloneMedia for Mouse Hybridoma Generation is a complete solution that supports all stages of hybridoma cell line development from fusion to scale up. Optimized to support the selection and growth of hybridoma clones using the Molecular Devices ClonePix systems, the kit is also compatible with other appropriate methods.

Key advantages:

  • The semi-solid CloneMedia method of cloning prevents the overgrowth of fast-growing clones, allowing for the selection of potentially valuable slow-growing clones
  • Hands-on time can be reduced when the workflow is combined with a ClonePix system. Hypoxanthine-aminopterin-thymidine (HAT) selection and cloning of hybridomas are accomplished in one step, minimizing the required time and materials
  • Ready to use (no extra addition of supplements required)

XP Media Pre-Fusion Myeloma Growth Medium and Hybridoma Expansion Medium (without HT)

XP Media Hybridoma Fusion Medium

XP Media Hybridoma Fusion Recovery Medium

CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (with HAT)

XP Media Hybridoma Growth Medium (with HT)

Hybridoma Polyethylene Glycol (PEG) for Cell Fusion

CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (without HAT)

Technology of XP Media and CloneMedia for Mouse Hybridoma Generation

  • Hybridoma Cell Development Workflow

    Workflow for antibody discovery in hybridomas

    The traditional approach to generating monoclonal antibody-producing cells (i.e. hybridomas) is to fuse myeloma cells with desired antibody-producing splenocytes (e.g. B cells). These B cells are typically sourced from animals, usually mice. Following cell fusion, large numbers of clones are screened and selected on the basis of antigen specificity and immunoglobulin class. Once candidate cell line clones are identified, each "hit" is re-confirmed, validated, and characterized using a variety of downstream functional assays. Once completed, the clones are expanded or scaled up where additional downstream bioprocesses occur.

    Here we provide a general overview of the hybridoma workflow showing where our XP Media and CloneMedia would be used in this hybridoma generation and scale-up process.

    Download Infographic 

Ordering Option of XP Media and CloneMedia for Mouse Hybridoma Generation

XP Media and CloneMedia Complete Kit for Mouse Hybridoma Generation

Complete Kit

Includes Pre-fusion media (K8862), Fusion media (K8863), Fusion Recovery media (K8864), Semi-solid (with HAT) media (K8865), Growth media (K8866), and PEG (K8868).

#K8861

$954.00 USD

1

XP Media Pre-Fusion Myeloma Growth Medium and Hybridoma Expansion Medium (without HT)

500 mL

Supports the growth of myeloma cells before fusion. Also supports expansion of hybridoma clones. Does not contain hypoxanthine or thymidine (HT).

#K8862

$119.00 USD

1

XP Media Hybridoma Fusion Medium

500 mL

Used to wash cells before fusion and during fusion process. Does not contain supplements to support growth. 

#K8863

$98.00 USD

1

XP Media Hybridoma Fusion Recovery Medium

100 mL

Used to promote hybridoma viability after the fusion process but before clone selection. Does not contain hypoxanthine, aminopterin, and thymidine (HAT).

#K8864

$116.00 USD

1

CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (with HAT)

90 mL

CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (with HAT)
Used after fusion of splenocytes and myeloma cells to select and clone hybridomas in one step. Methycellulose-based formula optimized for colony formation. Equally suitable for fresh fusions and for stable hybridoma cell lines.

 

 

#K8865

$433.00 USD

1

XP Media Hybridoma Growth Medium (with HT)

500 mL

Optimized for hybridoma expansion following clone selection and colony picking. Contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin from the selection process. 

 

#K8866

$291.00 USD

1

CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (without HAT)

90 mL

If you are using alternative hybridoma selection methods, then this medium will be perfect for your needs. Researchers can add desired selection reagents into this medium and use to select and clone hybridomas in one step after fusion. The single cell-derived hybridomas are immobilized in the semi-solid medium as they grow to form monoclonal colonies.

 

 

#K8867

$433.00 USD

1

Hybridoma Polyethylene Glycol (PEG) for Cell Fusion

1.5 mL

Used for the fusion of mouse splenocytes and parental myeloma cells to generate hybridomas. PEG is present as a 50% solution in DMEM. K8868

#K8868

$39.00 USD

1

Resources of XP Media and CloneMedia for Mouse Hybridoma Generation

Videos and Demos

Hybridoma Selection using HAT Medium

Hybridoma selection using HAT medium

Preparing and Plating Cells in Semi Solid Medium

Preparing and Plating Cells in Semi-Solid Medium

XP Media and CloneMedia for Mouse Hybridoma Generation

XP Media and CloneMedia for Mouse Hybridoma Generation

Compatible Products & Services of XP Media and CloneMedia for Mouse Hybridoma Generation

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.