Overview of XP Media and CloneMedia for Mouse Hybridoma Generation
The XP Media and CloneMedia for Mouse Hybridoma Generation is a complete solution that supports all stages of hybridoma cell line development from fusion to scale up. Optimized to support the selection and growth of hybridoma clones using the Molecular Devices ClonePix systems, the kit is also compatible with other appropriate methods.
- The semi-solid CloneMedia method of cloning prevents the overgrowth of fast-growing clones, allowing for the selection of potentially valuable slow-growing clones
- Hands-on time can be reduced when the workflow is combined with a ClonePix system. Hypoxanthine-aminopterin-thymidine (HAT) selection and cloning of hybridomas are accomplished in one step, minimizing the required time and materials
- Ready to use (no extra addition of supplements required)
XP Media Pre-Fusion Myeloma Growth Medium and Hybridoma Expansion Medium (without HT)
XP Media Hybridoma Fusion Medium
XP Media Hybridoma Fusion Recovery Medium
CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (with HAT)
XP Media Hybridoma Growth Medium (with HT)
Hybridoma Polyethylene Glycol (PEG) for Cell Fusion
CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium (without HAT)
Technology of XP Media and CloneMedia for Mouse Hybridoma Generation
Workflow for antibody discovery in hybridomas
The traditional approach to generating monoclonal antibody-producing cells (i.e. hybridomas) is to fuse myeloma cells with desired antibody-producing splenocytes (e.g. B cells). These B cells are typically sourced from animals, usually mice. Following cell fusion, large numbers of clones are screened and selected on the basis of antigen specificity and immunoglobulin class. Once candidate cell line clones are identified, each "hit" is re-confirmed, validated, and characterized using a variety of downstream functional assays. Once completed, the clones are expanded or scaled up where additional downstream bioprocesses occur.
Here we provide a general overview of the hybridoma workflow showing where our XP Media and CloneMedia would be used in this hybridoma generation and scale-up process.
Resources of XP Media and CloneMedia for Mouse Hybridoma Generation
XP Media and CloneMedia for Mouse Hybridoma Generation provides a complete solution that supports all stages of hybridoma cell line development. The media are optimized to support the…
Explore CloneMedia & XP Media suite of hybridoma media that offers a complete solution for generating, culturing, cloning, screening and scaling up of hybridomas.
Explore FAQs related to XP Media and CloneMedia for mouse hybridoma generation.
Explore complete workflow of hybridoma cell line development, where workflow starts from pre-fusion to clone selection, functional characterization, scale up & wean to expand.
Get an overview of the complete solution for high-throughput hybridoma cloning and screening for antibody discovery provided by Molecular Devices. The solution is also being compared to…
Hybridomas cultured in semi-solid media are immobilized allowing them to grow and form distinct clonal colonies. This improves laboratory efficiency by making it possible to seed and sc…
Videos and Demos
Hybridoma selection using HAT medium
Preparing and Plating Cells in Semi-Solid Medium
XP Media and CloneMedia for Mouse Hybridoma Generation
Compatible Products & Services of XP Media and CloneMedia for Mouse Hybridoma Generation
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