Eyewitness proof of monoclonality

The CloneSelect™ Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge. Sort cells using high-res imaging in either bright field or optional fluorescence and capture 5 images per each single cell deposit. Prove monoclonality, improve your efficiency, maintain and enrich your viability, and prevent cross-contamination.

  • Image

    Cell isolation proof captured

    Document a sequence of five images as the cell is dispensed, providing direct image evidence of monoclonality in 96- or 384-well plate formats.

  • Efficient

    Clonal outgrowth improved

    Achieve up to 8X improvement in clonal outgrowth efficiency over traditional methods. Deposit single cells with high efficiency into 96- or 384-well plates. It takes as little as ~5 min to dispense into a 96-well plate.

  • Select

    Cells stay healthy and clean

    Maintain cell viability with gentle sorting as seen with outgrowth assays, and prevent cross contamination with sterile, disposable one-way flow cartridge.

CloneSelect Single-Cell Printer

Features

  • Image-Gn

    Image-based proof

    Five images are captured each time a single-cell is dispensed, providing direct, image-based evidence of monoclonality that can be used in regulatory fillings.

  • Image-Gn

    Fluorescence imaging and sorting

    Sort viable cells based on live-cell fluorescent dye, enrich cell populations for high producers with fluorescent reporter, or isolate desired fluorescent CRISPR clones.

  • Accuracy-Gn

    Microfluidics technology

    A patented, silicon microfluidic chip within the sterile cartridge generates pico-liter droplets that encapsulate cells. Only high-value droplets are seeded into plates.

  • Sterility-Gn

    Label-free imaging and sorting

    Sort cells based on size and morphology from a heterogenous cell population and select to deposit from 1 up to 100 cells per well.

  • Dispensing a wide variety of cell types

    A wide range of cell types such as CHO, HEK293, L929, t-cells and b-cells can be dispensed. Cells can range in size from 5 and 40 um.

  • Sterile Cartridge

    Individually-wrapped, sterile cartridges were designed so that cell samples never contact the instrument. A single cartridge can be used to print several microplates.

Applications of CloneSelect Single-Cell Printer Series

  • Cell Line Development for Recombinant Proteins

    Cell Line Development

    Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.

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  • single-cell-genomics

    Single-Cell Genomics

    The isolation of single cells remains a challenging task in single-cell genomics. Current methods lack the evidence that only a single-cell has been isolated in the analysis vessel. It is important that the integrity of the cells is maintained prior to their lysis in order to preserve their DNA.

    Furthermore, cells should undergo as little stress as possible prior to their lysis in order to preserve their RNA and its expression level. The CloneSelect Single-Cell Printer Series deposits single cells in a very gentle manner, guaranteeing high purity and high viability.

    This provides optimal basis for downstream single-cell genomic analysis.

  • Cell Line Development and Monoclonality Assurance

    Monoclonality Assurance

    Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.

    Read Application Note 

  • Single Cell Sorting with CloneSelect Single Cell Printer

    Single-Cell Sorting

    Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability, but is time consuming. The CloneSelect Single-Cell Printer enables the gentle isolation of single cells in a manner that maximizes cell viability, as well as provides direct evidence of clonality via a series of five images captured for each dispense of a single cell.

Specifications & Options of CloneSelect Single-Cell Printer Series

 

** Can address a cell culture 96-well plate with a suspension of 10 μm beads in sterile filtered water (concentration about 5x105 to 1x106 beads/ml) in <5 min in brightfield and <10 min in fluorescent sorting (f.sight only) by using optimal parameters for fast sorting by the user.

Resources of CloneSelect Single-Cell Printer Series

Videos and Demos

CloneSelect Single-Cell Printer

Microfluidics Based Single Cell Isolation Workflow for Assurance of Monoclonality

A Microfluidics-Based Single Cell Isolation Workflow Optimized for Efficiency, Viability, and Assurance of Monoclonality

CloneSelect Single-Cell Printer Series

 

Available from Molecular Devices in North America, for all other countries please contact Cytena directly info@cytena.com

 

Product Part Number
CloneSelect Single-Cell Printer f.sight Instrument CLONESELECT-F.SIGHT
CloneSelect Single-Cell Printer c.sight Instrument CLONESELECT-C.SIGHT
CloneSelect Single-Cell Printer SCP Instrument CLONESELECT-SCP

Related Products & Services of CloneSelect Single-Cell Printer Series

Featured Applications

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Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.