High throughput Glycan Screening of Crude and Purified Samples

GlyS

Figure 1: Example assay workflow for human IgG or human Fc-fusion proteins. Selective amplification of signal is from the protein of interest and not from host-cell protein (HCP).

Glycosylation is considered among the most important post-translational modifications when developing new biologics. Having a significant impact on product performance and variability, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy. Protein glycosylation can affect isolation and purification steps (process consistency), pharmacokinetics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

ForteBio’s Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined with titer data using Octet Data Analysis HT software version 11.1 or higher to funnel through samples that are high producers and have desirable sialic acid content.

Assay Parameter Octet RED96e System, Sidekick and GlyS Kit Octet RED384 System, Sidekick and GlyS Kit Octet HTX System and GlyS Kit
Time to Results <2.5 hr/96 samples ˜1.5 hr/96 samples <1 hr/96 samples
Sample Type Crude cell culture supernatant Crude cell culture supernatant Crude cell culture supernatant

Table 1: Comparison of GlyS throughput on various Octet systems.

Ordering Options of Sialic Acid (GlyS) Kit

Sialic Acid (GlyS) Kit

Sialic Acid (GlyS) Kit

#18-5135

$1,250.00 USD

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Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Maximize flexibility in application workflow and experimental design

Contact our specialist today to discuss how to implement a solution that works for you