Neurotransmitter Transporter Uptake Assay Kit
Until recently, radioactively labeled compounds were used to measure serotonin, norepinephrine and dopamine transporter uptake. With the introduction of the Neurotransmitter Transporter Uptake Assay Kit, researchers now have a tool to screen for live-cell kinetic uptake for these three key neurotransmitters.
- Homogeneous, live-cell fluorescent assay replaces radioactive assays, reducing disposal costs and eliminating safety risks associated with radiolabeled assays
- One-step, homogeneous assay format is amenable to automation and high-throughput screening
- Two assay setup options offer greater flexibility: endpoint mode for high-throughput applications, or real-time kinetic mode for mechanistic studies
Technology of Neurotransmitter Transporter Uptake Assay Kit
The Neurotransmitter Transporter Uptake Assay Kit uses a fluorescent substrate that mimics the biogenic amine neurotransmitters and is taken into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity that is monitored in real time using a bottom-reading microplate reader. In this convenient no-wash procedure, cells are incubated with the kit reagent and transferred to the plate reader for evaluation. The assay is ideal for use in academic, biotech, and pharmaceutical environments for research, assay development, and screening.
For more details, please refer to the Neurotransmitter Transporter Uptake Assay Kit product insert, which may be accessed through the Molecular Devices Knowledge Base.
Resources of Neurotransmitter Transporter Uptake Assay Kit
Read this PDF to know how fluorescence-based assay kit is useful for the measurement of neurotransmitter transporter uptake activity in cell lines stably expressing the human DAT, NET,…
Here we present a homogeneous assay of norepinephrine, dopamine, and serotonin transporter activity based on cellular uptake of a fluorescent dye coupled with a proprietary masking dye.…
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