Overview of SpectraMax DuoLuc Reporter Assay Kit

The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price.

  • Flash-type luminescence with excellent sensitivity
  • Expression measurement in as few as ten cells per well
  • Improved accuracy of results by normalizing reporter expression to a control
  • Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors
  • Simplified data acquisition and analysis with preconfigured protocol in SoftMax® Pro Software
  • Support for your complete solution of reader, software, and assay

Technology of SpectraMax DuoLuc Reporter Assay Kit

  • Principle of SpectraMax DuoLuc Assay Kit

    Assay Kit Mechanism

    Reporter gene assays are an important tool for monitoring cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP. Renilla luciferase catalyzes coelenterazine oxidation to produce light. Due to the difference in substrate requirements, it is possible to measure the activity of both luciferases in a single sample through sequential addition of the substrates. The result is a convenient assay with two readouts that can be normalized to each other to provide more comprehensive information about a cellular pathway or event.

  • Experimental Workflow for Dual Luciferase Assay

    Assay Kit Workflow

    Representative experimental workflow for dual luciferase assay.

  • SpectraMax DuoLuc assay with 96-well Microplate

    Assay Kit Protocol

    SpectraMax DuoLuc assay with 96-well microplate

Ordering Options of SpectraMax DuoLuc Reporter Assay Kit

SpectraMax® DuoLuc™ Reporter Assay Kit

Evaluation Kit (50 reactions)

#R8360

$129.00 USD

1

Explorer Kit (200 reactions)

#R8361

$299.00 USD

1

Bulk Kit (1000 reactions)

#R8362

$1,099.00 USD

1

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.