Overview of SpectraMax DuoLuc Reporter Assay Kit
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price.
- Flash-type luminescence with excellent sensitivity
- Expression measurement in as few as ten cells per well
- Improved accuracy of results by normalizing reporter expression to a control
- Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors
- Simplified data acquisition and analysis with preconfigured protocol in SoftMax® Pro Software
- Support for your complete solution of reader, software, and assay
Latest Resources
Data of SpectraMax DuoLuc Reporter Assay Kit
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accurate and sensitive measurement of dual luciferase expression over a wide dynamic range
Dilution series of purified firefly (red plot) and Renilla (blue plot) luciferases.
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Linear correlation to transfected HeLa cell counts
Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases.
Technology of SpectraMax DuoLuc Reporter Assay Kit
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Assay Kit Mechanism
Reporter gene assays are an important tool for monitoring cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP. Renilla luciferase catalyzes coelenterazine oxidation to produce light. Due to the difference in substrate requirements, it is possible to measure the activity of both luciferases in a single sample through sequential addition of the substrates. The result is a convenient assay with two readouts that can be normalized to each other to provide more comprehensive information about a cellular pathway or event.
Assay Kit Protocol
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SpectraMax DuoLuc assay with 96-well microplate
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Assay Kit Workflow
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Representative experimental workflow for dual luciferase assay.
Resources of SpectraMax DuoLuc Reporter Assay Kit
Publications
The Best Microplate Readers According to our Product Reviewers
The Best Microplate Readers According to our Product Reviewers
According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. While they play an important role in generating data from a wide range o…
Product Insert
SpectraMax DuoLuc Reporter Assay Kit
SpectraMax DuoLuc Reporter Assay Kit
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells. Serial injection of two optimized detecti…
Brochure
Optimized assay kits for life science research and drug discovery
Optimized assay kits for life science research and drug discovery
The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Tailored to your different needs, these kits ar…
Data Sheet
SpectraMax DuoLuc Reporter Assay Kit
SpectraMax DuoLuc Reporter Assay Kit
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells. Serial injection of two optimized detecti…
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Application Note
Detect dual luciferase expression on the FlexStation 3 microplate reader
Detect dual luciferase expression on the FlexStation 3 microplate reader
Reporter gene assays are important tools for studying gene expression associated with the activation of cellular pathways. Cells are transfected with a plasmid containing the reporter…
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Application Note
Highly sensitive dual luciferase detection with the SpectraMax DuoLuc Reporter Assay Kit
Highly sensitive dual luciferase detection with the SpectraMax DuoLuc Reporter Assay Kit
Firefly luciferase is a widely used reporter to study gene regulation and function. It is a very sensitive reporter due to the lack of any endogenous luciferase activity in mammalian…
Compatible Products & Services of SpectraMax DuoLuc Reporter Assay Kit
Featured Applications
Assay Kit Mechanism
Reporter gene assays are an important tool for monitoring cellular events associated with gene…
Assay Kit Protocol
Please click here to enlarge image. SpectraMax DuoLuc assay with 96-well microplate
Assay Kit Workflow
Please click here to enlarge image. Representative experimental workflow for dual luciferase assay.
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