Overview of SpectraMax DuoLuc Reporter Assay Kit
The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price.
- Flash-type luminescence with excellent sensitivity
- Expression measurement in as few as ten cells per well
- Improved accuracy of results by normalizing reporter expression to a control
- Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors
- Simplified data acquisition and analysis with preconfigured protocol in SoftMax® Pro Software
- Support for your complete solution of reader, software, and assay
Technology of SpectraMax DuoLuc Reporter Assay Kit
Assay Kit Mechanism
Reporter gene assays are an important tool for monitoring cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP. Renilla luciferase catalyzes coelenterazine oxidation to produce light. Due to the difference in substrate requirements, it is possible to measure the activity of both luciferases in a single sample through sequential addition of the substrates. The result is a convenient assay with two readouts that can be normalized to each other to provide more comprehensive information about a cellular pathway or event.
Assay Kit Workflow
Representative experimental workflow for dual luciferase assay.
Assay Kit Protocol
SpectraMax DuoLuc assay with 96-well microplate
Resources of SpectraMax DuoLuc Reporter Assay Kit
Firefly luciferase is a widely used reporter to study gene regulation and function. It is a very sensitive reporter due to the lack of any endogenous luciferase activity in mammalian…
Reporter gene assays are important tools for studying gene expression associated with the activation of cellular pathways. Cells are transfected with a plasmid containing the reporter…
The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Tailored to your different needs, these kits are c…
Compatible Products & Services of SpectraMax DuoLuc Reporter Assay Kit
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