CatchPoint cGMP Fluorescent Assay Kit

The CatchPoint® cGMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cGMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.

Benefits of CatchPoint cGMP Fluorescent Assay Kit:

  • High affinity reagents are optimized for sensitivity when cGMP levels are low, ensuring results when other assays fail.
  • Robust format resists interference from colored or fluorescent compounds, improving reliability of results.
  • Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.
  • Quantitative method allows accurate determination of cGMP in samples.

Data of CatchPoint cGMP Fluorescent Assay Kit

  • CatchPoint-cGMP_fig1

    cGMP Calibration Curve

    Calibration curve, 96-well assay format. Calibration curve for the CatchPoint cyclic-GMP Kit. Data was taken 60 minutes after addition of Stoplight Red substrate. The EC50 of the cGMP calibration curve was 2.2 nM (264 fmol). Minimum detectable concentration was 0.2 nM (24 fmol).

  • Atrial Natriuretic Peptide Dose Response

    Atrial Natriuretic peptide dose response in RFL-6 cells, 96-well assay format. A confluent monolayer of RFL-6 cells in a 12-well plate was pre-incubated for 10 minutes in Krebs Ringer bicarbonate buffer containing the non-selective phosphodiesterase inhibitor IBMX. Cells were subsequently stimulated with ANP for 15 minutes, lysed, and analyzed using the cGMP Fluorescent Assay Kit.

    CatchPoint-cGMP_fig2

Technology of CatchPoint cGMP Fluorescent Assay Kit

  • CatchPoint cGMP Assay Principle

    CatchPoint cGMP Assay Principle

    The cGMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cGMP conjugate for binding sites on the anti-cGMP antibodies. In the absence of cGMP, most of the HRP-cGMP conjugate is bound to the antibody. Increasing concentrations of cGMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity

Ordering Options of CatchPoint cGMP Fluorescent Assay Kit

CatchPoint cGMP Fluorescent Assay Kit

Explorer Kit (96-Wells)

  • 2 96-well black-bottom plates
  • 100 mL ELISA Assay Buffer
  • 1 vial Rabbit Anti-cGMP Antibody
  • 1 vial cGMP Calibrator
  • 1 vial HRP-cGMP Conjugate
  • 100mL, 10X Wash Solution Concentrate
  • 50 mL Cell Lysis Buffer
  • 1 vial 100X Substrate
  • 100 mL Substrate Buffer

#R8074

$2,069.00 USD

1

Bulk Kit (96-Wells)

  • 10 96-well black-bottom plates
  • 950 mL ELISA Assay Buffer
  • 2 vials Rabbit Anti-cAMP Antibody
  • 2 vials cAMP Calibrator
  • 2 vials HRP-cAMP Conjugate
  • 950mL, 10X Wash Solution Concentrate
  • 450 mL Cell Lysis Buffer
  • 2 x 900 µL 100X Substrate
  • 950 mL Substrate Buffer

#R8075

$3,594.00 USD

1

Explorer Kit (384-Wells)

  • 2 384-well black-bottom plates
  • 100 mL ELISA Assay Buffer
  • 2 vials Rabbit Anti-cGMP Antibody
  • 2 vials cGMP Calibrator
  • 2 vials HRP-cGMP Conjugate
  • 100mL, 10X Wash Solution Concentrate
  • 50 mL Cell Lysis Buffer
  • 1 vial 100X Substrate
  • 100 mL Substrate Buffer

#R8065

$2,286.00 USD

1

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.