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CatchPoint cAMP Fluorescent Assay Kit
Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.
CatchPoint cAMP Fluorescent Assay Kit
cAMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cAMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.
Benefits of CatchPoint cAMP Fluorescent Assay Kit:
- High affinity reagents are optimized for sensitivity when cAMP levels are low, ensuring results when other assays fail.
- Robust format resists interference from colored or fluorescent compounds, improving reliability of results.
- Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.
- Quantitative method allows accurate determination of cAMP in samples.
Latest Resources
Data of CatchPoint cAMP Fluorescent Assay Kit
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cAMP Calibration Curve
The calibration curve for the CatchPoint® cAMP Assay Kit was generated on a SpectraMax® M5 Multi-Mode Microplate Reader. Data were taken 24 hours after addition of Stoplight Red substrate. The EC50of the cAMP calibration curve was 5.1 nM.
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Forskolin Dose Response in HEK293 Cells
10,000 cells were stimulated in a volume of 20 μL for 15 minutes, then lysed and analyzed using the CatchPoint cAMP Fluorescent Assay Kit. Data were generated on a SpectraMax M5 Multi-Mode Microplate Reader.
Technology of CatchPoint cAMP Fluorescent Assay Kit
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CatchPoint cAMP Assay Principle
The cAMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cAMP conjugate for binding sites on the anti-cAMP antibodies. In the absence of cAMP, most of the HRP-cAMP conjugate is bound to the antibody. Increasing concentrations of cAMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity.
Ordering Options of CatchPoint cAMP Fluorescent Assay Kit
CatchPoint cAMP Fluorescent Assay Kit
Explorer Kit (96-Wells) - PN: R8088
- Two (2) 96-well black well/ clear bottom plates
- 100 mL ELISA Assay Buffer
- 1 vial Rabbit Anti-cAMP Antibody
- 1 vial cAMP Calibrator
- 1 vial HRP-cAMP Conjugate
- 100mL, 10X Wash Solution Concentrate
- 50 mL Cell Lysis Buffer
- 1 vial 100X Substrate
- 100 mL Substrate Buffer
#R8088
Bulk Kit (96-Wells) - PN: R8089
- Ten (10) 96-well black well/ clear bottom plates
- 950 mL ELISA Assay Buffer
- 2 vials Rabbit Anti-cAMP Antibody
- 2 vials cAMP Calibrator
- 2 vials HRP-cAMP Conjugate
- 950mL, 10X Wash Solution Concentrate
- 450 mL Cell Lysis Buffer
- 2 x 900 µL 100X Substrate
- 950 mL Substrate Buffer
#R8089
Explorer Kit (384-Wells) - PN: R8044
- Two (2) 384-well black well/ clear bottom plates
- 100 mL ELISA Assay Buffer
- 2 vials Rabbit Anti-cAMP Antibody
- 2 vials cAMP Calibrator
- 2 vials HRP-cAMP Conjugate
- 100mL, 10X Wash Solution Concentrate
- 25 mL Cell Lysis Buffer
- 1 vial 100X Substrate
- 100 mL Substrate Buffer
#R8044
Resources of CatchPoint cAMP Fluorescent Assay Kit
Application Note
Complete cAMP workflow solution using the CatchPoint cAMP Fluorescent Assay Kit
Complete cAMP workflow solution using the CatchPoint cAMP Fluorescent Assay Kit
G-Protein Coupled Receptors (GPCRs) are important transmembrane proteins that translate extracellular signals into intracellular responses. These intracellular responses are comprised…
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
Data Sheet
CatchPoint cAMP Fluorescent Assay Kit
CatchPoint cAMP Fluorescent Assay Kit
The CatchPoint® Cyclic-AMP Assay Kit measures cAMP levels and adenylate cyclase activity via a competitive immunoassay format. The assay’s high affinity reagents are optimized for se…
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Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
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How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…