Overview of SpectraMax Quant dsDNA Assay Kits

Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next-generation sequencing. Compared to spectrophotometric DNA quantification, the fluorometric method provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use.

  • Three kits covering a wide range of dsDNA concentrations
  • Highly selective for dsDNA over ssDNA or RNA
  • High tolerance for contaminants
  • Optimized for fluorescence microplate readers
  • Simplified data acquisition and analysis with preconfigured protocols in SoftMax Pro Software
Assay Kit

Linear detection range (per well)

Fluorescence reader setting
Excitation/Emission

SpectraMax® Quant™ AccuBlue™ Pico dsDNA Assay Kit 5 pg – 3 ng 468 /507 nm
SpectraMax® Quant™ AccuClear™ Nano dsDNA Assay Kit 30 pg – 250 ng  468 /507 nm
SpectraMax® Quant™ AccuBlue™ HiRange dsDNA Assay Kit 2 ng – 2,000 ng 350 /460 nm

Data of SpectraMax Quant dsDNA Assay Kits

  • SpectraMax Quant_Optimal Data Quality

    Standard curves

    Standard curve for the three SpectraMax Quant dsDNA Assay kits.(A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C) AccuBlue HiRange kit.

  • dsDNA selectivity

    Selectivity for dsDNA for three SpectraMax Quant dsDNA Assay kits. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C) AccuBlue HiRange kit.

    SpectraMax Quant_High Selectivity for dsDNA

Technology of SpectraMax Quant dsDNA Assay Kits

  • SpectraMax Quant dsDNA Assay Kit Mechanism

    Assay Kit Mechanism

    The SpectraMax Quant dsDNA Assay Kits consist of unique dyes that selectively bind to dsDNA and yield bright fluorescent signals. An enhancer is also included to further reduce the background fluorescence and increase the dynamic range of detection. The three specially formulated kits enable accurate and precise measurement of dsDNA from picogram (pg) to thousands of nanogram (ng).

  •  SpectraMax Quant dsDNA Assay kit workflow

    Assay Kit Workflow

    SpectraMax Quant dsDNA Assay kit workflow.

Ordering Options of SpectraMax Quant dsDNA Assay Kits

SpectraMax® Quant™ AccuClear™ Nano dsDNA Assay Kit

Explorer Kit

Fluorescence-based DNA assay kit that detects dsDNA from 30 pg to 250 ng.
Sufficient for (2) 96-well plates.

#R8356

$100.00 USD

1

Bulk Kit

Fluorescence-based DNA assay kit that detects dsDNA from 30 pg to 250 ng.
Sufficient for (10) 96-well plates.

#R8357

$320.00 USD

1

SpectraMax® Quant™ AccuBlue™ HiRange dsDNA Assay Kit

Explorer Kit

Fluorescence-based DNA assay kit that detects dsDNA from 2 ng to 2,000 ng.
Sufficient for (2) 96-well plates.

#R8358

$110.00 USD

1

Bulk Kit

Fluorescence-based DNA assay kit that detects dsDNA from 2 ng to 2,000 ng.
Sufficient for (10) 96-well plates.

#R8359

$330.00 USD

1

SpectraMax® Quant™ AccuBlue™ Pico dsDNA Assay Kit

Explorer Kit

Fluorescence-based DNA assay kit that detects dsDNA from 5 pg to 3 ng.
Sufficient for (2) 96-well plates.

#R8354

$130.00 USD

1

Bulk Kit

Fluorescence-based DNA assay kit that detects dsDNA from 5 pg to 3 ng.
Sufficient for (10) 96-well plates.

#R8355

$390.00 USD

1

Resources of SpectraMax Quant dsDNA Assay Kits

Compatible Products & Services of SpectraMax Quant dsDNA Assay Kits

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.