Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures

complex event analysis


We describe a method for the complex analysis of calcium oscillations using the FLIPR® Penta High-Throughput Cellular Screening System that allows detection and multi-parametric characterization of oscillation peaks. ScreenWorks® Peak Pro 2 analysis software analyzes calcium oscillation rate, peak width and amplitude, characterization of secondary peaks, waveform irregularities, and several other read-outs.

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