(A) Untreated cells (left panel) are mostly alive, with red fluorescent nuclei. At an intermediate concentration of compound (center panel), there is a mixture of live and dead cells. At high compound concentration (right panel), most cells are dead, with nuclei labeled both red and green. Images were acquired on the SpectraMax® MiniMax™ 300 Imaging Cytometer. (B) Cells were identified as live (red masks) or dead (blue masks) using the Classification feature in SoftMax® Pro Software.