## Life Sciences Solutions ![Scientist using SpectraMax iD3s/iD5e Microplate Rreader](https://www.moleculardevices.com/media_19ab0db8903379155be955f9aaf708a8a346e28d2.png?width=750&format=png&optimize=medium) ![CellXpress.ai System](https://www.moleculardevices.com/media_1abc692a373219773cd4c045acfb8daff6e2ce892.jpg?width=750&format=jpg&optimize=medium) [![ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/media_117b5f76343ad362b4891db7b30ff7861ec0cfee2.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai") ### Dive Deeper: **ImageXpress HCS.ai High-Content Screening System** with AI-powered Insights _[Deeper insights for every lab](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "Deeper insights for every lab")_ [![QPix FLEX Microbial Colony Picking System](https://www.moleculardevices.com/media_161f952326374aece2821250eb880bf5075877429.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system "https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system") ### See the difference. Pick the best. **QPix FLEX Microbial Colony Picking System** _[See the difference](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system "See the difference")_ [![SpectraMax® iD3s & iD5e Multi-Mode Microplate Readers](https://www.moleculardevices.com/media_1c3d682bf05193400ab5a7c7bb251505974537568.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers "https://main--moleculardevices--hlxsites.aem.page/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers") ### Next-Gen Readers. Limitless Discovery. **SpectraMax® iD3s & iD5e Multi-Mode Microplate Readers** _[Explore your next-gen reader](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers "Explore your next-gen reader")_ [![ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/media_117b5f76343ad362b4891db7b30ff7861ec0cfee2.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai") ### Dive Deeper: **ImageXpress HCS.ai High-Content Screening System** with AI-powered Insights _[Deeper insights for every lab](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "Deeper insights for every lab")_ [![QPix FLEX Microbial Colony Picking System](https://www.moleculardevices.com/media_161f952326374aece2821250eb880bf5075877429.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system "https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system") ### See the difference. Pick the best. **QPix FLEX Microbial Colony Picking System** _[See the difference](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system "See the difference")_ [![SpectraMax® iD3s & iD5e Multi-Mode Microplate Readers](https://www.moleculardevices.com/media_1c3d682bf05193400ab5a7c7bb251505974537568.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers "https://main--moleculardevices--hlxsites.aem.page/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers") ### Next-Gen Readers. Limitless Discovery. **SpectraMax® iD3s & iD5e Multi-Mode Microplate Readers** _[Explore your next-gen reader](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers "Explore your next-gen reader")_ [![ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/media_117b5f76343ad362b4891db7b30ff7861ec0cfee2.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai") ### Dive Deeper: **ImageXpress HCS.ai High-Content Screening System** with AI-powered Insights _[Deeper insights for every lab](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-hcs-ai "Deeper insights for every lab")_ ## A life science company ### We journey alongside researchers, combining next-gen technology and deep expertise to spark bold breakthroughs that push research boundaries and transform lives worldwide. [![Molecular Devices Unveils Next-Generation SpectraMax Multi-Mode Microplate Readers](https://www.moleculardevices.com/newsroom/news/media_1b06dabd8057efd3de8a505143418b04f2cb9926a.jpg?width=800&format=jpg&optimize=medium)\\ \\ Jul 15, 2025\\ \\ Molecular Devices Unveils Next-Generation SpectraMax Multi-Mode Microplate Readers](https://www.moleculardevices.com/newsroom/news/next-gen-spectramax-multi-mode-microplate-readers) [![Molecular Devices Launches Automated QPix FLEX Microbial Colony Picking System](https://www.moleculardevices.com/newsroom/news/media_1c2d8925f4cbbbb385759670d22b0c59c97424709.jpg?width=800&format=jpg&optimize=medium)\\ \\ May 06, 2025\\ \\ Molecular Devices Launches Automated QPix FLEX Microbial Colony Picking System](https://www.moleculardevices.com/newsroom/news/qpix-flex-microbial-colony-picking-system-launch) [![Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/newsroom/news/media_1e10a63a7266b3a8a7f6725d51bf0cbf8dc9dcae9.jpg?width=800&format=jpg&optimize=medium)\\ \\ Jan 16, 2025\\ \\ Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) [![Molecular Devices Launches AI-Powered QPix Insights App for Microbial Colony Picking](https://www.moleculardevices.com/newsroom/news/media_176745c8d38d5f3d987383a219b4bde1f6c1e57fc.jpg?width=800&format=jpg&optimize=medium)\\ \\ Aug 05, 2024\\ \\ Molecular Devices Launches AI-Powered QPix Insights App for Microbial Colony Picking](https://www.moleculardevices.com/newsroom/news/molecular-devices-launches-ai-powered-qpix-insights-app-for-microbial-colony-picking) [![Nobel Laureate Professor Sir Martin Evans opens Molecular Devices’ Cardiff facility to manufacture patient-derived organoids for research and drug discovery](https://www.moleculardevices.com/newsroom/news/media_1d5c9fed69d94baf40aa0e3453af3d343e5395bfa.jpeg?width=800&format=jpeg&optimize=medium)\\ \\ Jun 05, 2024\\ \\ Nobel Laureate Professor Sir Martin Evans opens Molecular Devices’ Cardiff facility to manufacture patient-derived organoids for research and drug discovery](https://www.moleculardevices.com/newsroom/news/molecular-devices-cardiff-facility-manufacture-patient-derived-organoids-for-research-and-drug-discovery) ## Advancing scientific discovery ### Unlocking the full potential of complex biology, human-relevant models, AI-driven discovery, and next-generation therapeutics is key to navigating what's next in life sciences. We partner with you to pave the way. ![BG Image](https://www.moleculardevices.com/media_1af3857c75e9fe4e17bd5581ccf9c77838493ad18.png?width=750&format=png&optimize=medium) - [For What’s Next](https://www.moleculardevices.com/for-whats-next) - [Exploring Complex Biology Together](https://www.moleculardevices.com/for-whats-next/exploring-complex-biology) - [Shifting Paradigms Together](https://www.moleculardevices.com/for-whats-next/shifting-paradigms-together) - [Transforming Science Together](https://www.moleculardevices.com/for-whats-next/transforming-science) - [Validating Next-Gen Therapeutics Together](https://www.moleculardevices.com/for-whats-next/validating-next-gen-therapeutics) ![for what’s next](https://www.moleculardevices.com/media_123b30c9f7750cfdd692b4fc1059b9dbffa526ca8.png?width=750&format=png&optimize=medium) For What’s Next ### We believe in the power of collaboration. Today’s scientific breakthroughs demand more than just advanced tools. They require partnerships built on understanding, commitment, and trust. Collaboration in life sciences plays a critical role in fueling advancements and unlocking new frontiers in discovery. At Molecular Devices, we stand shoulder to shoulder with customers as indispensable partners. Whether enabling the adoption of complex biology, supporting human-relevant model research, integrating AI and machine learning, or validating the next generation of therapeutic modalities, we empower transformational breakthroughs. _[Are you ready for what’s next?](https://www.moleculardevices.com/for-whats-next "Are you ready for what’s next?")_ ![Exploring Complex Biology](https://www.moleculardevices.com/media_1886ce581f379f345d0ce8be1139cf1bff993ac9b.png?width=750&format=png&optimize=medium) Exploring Complex Biology Together ### Unlocking the full potential of complex biology to revolutionize research. Our industry expertise and advanced technologies are unlocking the potential for complex biology to revolutionize scientific research. We partner with customers to create tailored, automated workflows that streamline complexity, increase throughput, and accelerate breakthroughs with confidence. _[Lab Automation](https://www.moleculardevices.com/service-support/lab-automation-solutions "Lab Automation")_ _[Organoid Innovation Center](https://www.moleculardevices.com/applications/organoid-innovation-center "Organoid Innovation Center")_ ![Making Human-relevant Model-based Research](https://www.moleculardevices.com/media_1f61fff07932c2bf9d5c5aa34fc372ea00e3f9ffd.png?width=750&format=png&optimize=medium) Shifting Paradigms Together ### Making human-relevant model-based research more accessible. Complex cell models mirroring human biology are key to reducing the persistently high attrition rate of drug candidates in clinical trials. From automated cell culture systems and screening solutions to an abundance of ready-for-use organoids from our lines or yours, we enable researchers to reliably assess the safety and efficacy of therapies based on human-relevant models from the outset. _[3D Ready Organoids](https://www.moleculardevices.com/products/3d-biology#3d-ready-organoids-and-organoid-expansion-service-1 "3D Ready Organoids")_ _[Automated Cell Culture System](https://www.moleculardevices.com/products/3d-biology/cellxpress-ai-automated-cell-culture-system "Automated Cell Culture System")_ _[Clone Screening](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-clone-screening "Clone Screening")_ ![Transforming Science -Power of AI and Machine](https://www.moleculardevices.com/media_1b536deefef313e5d0d08b29ee2cb10a37542b190.png?width=750&format=png&optimize=medium) Transforming Science Together ### Unleashing the power of AI and machine learning to advance discovery. Harnessing AI and machine learning puts scientists in the strongest ever position to industrialize and interrogate complex biology. From cell culturing to smart imaging to advanced data analysis, our automated, AI-enabled, end-to-end solutions free researchers from making regular, mundane checks so they can invest their time on transformative science. _[High Content Imaging](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging "High Content Imaging")_ _[High Content Analysis](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis "High Content Analysis")_ ![Validating Next-Gen Therapeutics](https://www.moleculardevices.com/media_160d692962c7eb1fe4a41fa11a63a6a939d804ee6.png?width=750&format=png&optimize=medium) Validating Next-Gen Therapeutics Together ### Assuring validation to accelerate time to market for new therapeutics If new therapeutic modalities are to realize their promise, traceable audit-ready software and hardware must evolve alongside them. Our solutions offer the data integrity needed to accelerate next-generation therapies. _[Microplate Readers](https://www.moleculardevices.com/products/microplate-readers "Microplate Readers")_ _[SoftMax Pro GxP Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software "SoftMax Pro GxP Software")_ _[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")_ ## Your indispensable partner in complex biology ### Our advanced instruments and automated workflows—built alongside you—unlock new possibilities, streamlining complexity so you can focus on discovery. ![SpectraMax Mini and Microplate reader MultiWash Bundle](https://www.moleculardevices.com/media_1e462b817a5fb520c79ae9c0fe93b169b6e28283d.png?width=750&format=png&optimize=medium) ### Save on a bundle: Microplate reader and washer Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate reader and washer solution: **[SpectraMax Mini + MultiWash Bundle](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-mini-reader#save-time-and-money-with-a-microplate-reader-and-washer-bundle "SpectraMax Mini + MultiWash Bundle")** _[SpectraMax iD3s/iD5e + AquaMax Bundle](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers#save-time-and-money-with-a-microplate-reader--washer-bundle "SpectraMax iD3s/iD5e + AquaMax Bundle")_ ![Lab automation for high-throughput plate-based assays](https://www.moleculardevices.com/media_1049fa031e6db1bb23f5d6202f14bc542f70c0e84.png?width=750&format=png&optimize=medium) ### Lab automation for high-throughput plate-based assays Explore our fully-integrated, automated workflow solutions for cell and biochemical assays. Our scalable ELISA Workcells provide walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. **[ELISA Workcells](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-plate-based-assays "ELISA Workcells")** ![SpectraMax Microplate Readers and SoftMax Pro Software](https://www.moleculardevices.com/media_1428274838b802784ee49399414f0de21f85f9916.png?width=750&format=png&optimize=medium) ### Easy-to-use, intuitive, configurable microplate readers For over 40 years, we’ve partnered with scientists to expand the boundaries of their research. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking barriers to novel, landmark discoveries. **[SpectraMax Microplate Readers](https://www.moleculardevices.com/products/microplate-readers "SpectraMax Microplate Readers")** _[SoftMax Pro Software](https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-software/softmax-pro-software "SoftMax Pro Software")_ ![GxP compliance journey for GMP/GLP labs](https://www.moleculardevices.com/media_131656c4dab4e645d3476d93f53728167ceb7a35e.png?width=750&format=png&optimize=medium) ### Streamline your GxP compliance journey for GMP/GLP labs A leader in comprehensive GxP compliance solutions, we combine our SpectraMax® microplate readers, SoftMax® Pro GxP software, SpectraTest® Validation Plates and expert IQ/OQ/PM services to help laboratories operating under GMP and GLP regulations follow FDA or regional regulatory guidelines with confidence. **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _[SoftMax Pro GxP Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software#kickstart-your-compliance-journey-with-our-gxp-starter-bundle "SoftMax Pro GxP Software")_ ![SpectraMax Mini and Microplate reader MultiWash Bundle](https://www.moleculardevices.com/media_1e462b817a5fb520c79ae9c0fe93b169b6e28283d.png?width=750&format=png&optimize=medium) ### Save on a bundle: Microplate reader and washer Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate reader and washer solution: **[SpectraMax Mini + MultiWash Bundle](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-mini-reader#save-time-and-money-with-a-microplate-reader-and-washer-bundle "SpectraMax Mini + MultiWash Bundle")** _[SpectraMax iD3s/iD5e + AquaMax Bundle](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-id3s-id5e-readers#save-time-and-money-with-a-microplate-reader--washer-bundle "SpectraMax iD3s/iD5e + AquaMax Bundle")_ ![Lab automation for high-throughput plate-based assays](https://www.moleculardevices.com/media_1049fa031e6db1bb23f5d6202f14bc542f70c0e84.png?width=750&format=png&optimize=medium) ### Lab automation for high-throughput plate-based assays Explore our fully-integrated, automated workflow solutions for cell and biochemical assays. Our scalable ELISA Workcells provide walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. **[ELISA Workcells](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-plate-based-assays "ELISA Workcells")** ![SpectraMax Microplate Readers and SoftMax Pro Software](https://www.moleculardevices.com/media_1428274838b802784ee49399414f0de21f85f9916.png?width=750&format=png&optimize=medium) ### Easy-to-use, intuitive, configurable microplate readers For over 40 years, we’ve partnered with scientists to expand the boundaries of their research. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking barriers to novel, landmark discoveries. **[SpectraMax Microplate Readers](https://www.moleculardevices.com/products/microplate-readers "SpectraMax Microplate Readers")** _[SoftMax Pro Software](https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-software/softmax-pro-software "SoftMax Pro Software")_ ![GxP compliance journey for GMP/GLP labs](https://www.moleculardevices.com/media_131656c4dab4e645d3476d93f53728167ceb7a35e.png?width=750&format=png&optimize=medium) ### Streamline your GxP compliance journey for GMP/GLP labs A leader in comprehensive GxP compliance solutions, we combine our SpectraMax® microplate readers, SoftMax® Pro GxP software, SpectraTest® Validation Plates and expert IQ/OQ/PM services to help laboratories operating under GMP and GLP regulations follow FDA or regional regulatory guidelines with confidence. **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _[SoftMax Pro GxP Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software#kickstart-your-compliance-journey-with-our-gxp-starter-bundle "SoftMax Pro GxP Software")_ ![GxP Compliance journey for GMP/GLP labs](https://www.moleculardevices.com/media_131656c4dab4e645d3476d93f53728167ceb7a35e.png?width=750&format=png&optimize=medium) ### Streamline your GxP compliance journey for GMP/GLP labs A leader in comprehensive GxP compliance solutions, we combine our SpectraMax® microplate readers, SoftMax® Pro GxP software, SpectraTest® Validation Plates and expert IQ/OQ/PM services to help laboratories operating under GMP and GLP regulations follow FDA or regional regulatory guidelines with confidence. **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _[GxP Starter Bundle](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software#kickstart-your-compliance-journey-with-our-gxp-starter-bundle "GxP Starter Bundle")_ ![Lab automation for high-throughput plate-based assays](https://www.moleculardevices.com/media_1049fa031e6db1bb23f5d6202f14bc542f70c0e84.png?width=750&format=png&optimize=medium) ### Lab automation for high-throughput plate-based assays Explore our fully-integrated, automated workflow solutions for cell and biochemical assays. Our scalable ELISA Workcells provide walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. **[ELISA Workcells](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-plate-based-assays "ELISA Workcells")** ![SpectraMax Microplate Readers and SoftMax Pro Software](https://www.moleculardevices.com/media_1f436dde71b00094d1593978e20135e2dae97846b.png?width=750&format=png&optimize=medium) ### Easy-to-use, intuitive, configurable microplate readers For over 40 years, we’ve partnered with scientists to expand the boundaries of their research. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking barriers to novel, landmark discoveries. **[SpectraMax Microplate Readers](https://www.moleculardevices.com/products/microplate-readers "SpectraMax Microplate Readers")** _[SoftMax Pro Software](https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-software/softmax-pro-software "SoftMax Pro Software")_ ![GxP Compliance journey for GMP/GLP labs](https://www.moleculardevices.com/media_131656c4dab4e645d3476d93f53728167ceb7a35e.png?width=750&format=png&optimize=medium) ### Streamline your GxP compliance journey for GMP/GLP labs A leader in comprehensive GxP compliance solutions, we combine our SpectraMax® microplate readers, SoftMax® Pro GxP software, SpectraTest® Validation Plates and expert IQ/OQ/PM services to help laboratories operating under GMP and GLP regulations follow FDA or regional regulatory guidelines with confidence. **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _[GxP Starter Bundle](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software#kickstart-your-compliance-journey-with-our-gxp-starter-bundle "GxP Starter Bundle")_ ![Lab automation for high-throughput plate-based assays](https://www.moleculardevices.com/media_1049fa031e6db1bb23f5d6202f14bc542f70c0e84.png?width=750&format=png&optimize=medium) ### Lab automation for high-throughput plate-based assays Explore our fully-integrated, automated workflow solutions for cell and biochemical assays. Our scalable ELISA Workcells provide walkaway time, increasing throughput, effectiveness and efficiency of the assay procedure, and reproducibility. **[ELISA Workcells](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-plate-based-assays "ELISA Workcells")** ![SpectraMax Microplate Readers and SoftMax Pro Software](https://www.moleculardevices.com/media_1f436dde71b00094d1593978e20135e2dae97846b.png?width=750&format=png&optimize=medium) ### Easy-to-use, intuitive, configurable microplate readers For over 40 years, we’ve partnered with scientists to expand the boundaries of their research. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking barriers to novel, landmark discoveries. **[SpectraMax Microplate Readers](https://www.moleculardevices.com/products/microplate-readers "SpectraMax Microplate Readers")** _[SoftMax Pro Software](https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-software/softmax-pro-software "SoftMax Pro Software")_ ![GxP Compliance journey for GMP/GLP labs](https://www.moleculardevices.com/media_131656c4dab4e645d3476d93f53728167ceb7a35e.png?width=750&format=png&optimize=medium) ### Streamline your GxP compliance journey for GMP/GLP labs A leader in comprehensive GxP compliance solutions, we combine our SpectraMax® microplate readers, SoftMax® Pro GxP software, SpectraTest® Validation Plates and expert IQ/OQ/PM services to help laboratories operating under GMP and GLP regulations follow FDA or regional regulatory guidelines with confidence. **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _[GxP Starter Bundle](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software#kickstart-your-compliance-journey-with-our-gxp-starter-bundle "GxP Starter Bundle")_ ![CellXpress.ai® Automated Cell Culture System](https://www.moleculardevices.com/media_1a57bf20efcba7990c821cbe961d8b4775e885f92.png?width=750&format=png&optimize=medium) ![CellXpress.ai® Automated Cell Culture System](https://www.moleculardevices.com/media_104e5da8437539e4202f78524fc5e9d78ccf18834.png?width=750&format=png&optimize=medium) ### Introducing the revolutionary CellXpress.ai® Automated Cell Culture System An Al-driven cell culture innovation hub, the CellXpress.ai system automates processes, improves workflows, and makes assays more reliable and reproducible with machine learning-assisted monitoring, feeding, imaging and scheduling. **[See the future of cell culture](https://www.moleculardevices.com/products/3d-biology/cellxpress-ai-automated-cell-culture-system "See the future of cell culture")** ![3D Ready™ Organoid](https://www.moleculardevices.com/media_15d8100d4478809065a5266f3d458edeeb6cd9383.png?width=750&format=png&optimize=medium) ![3D Ready™ Organoid](https://www.moleculardevices.com/media_1cea19723f5601df8fbc2579e635c04e0f164c3b1.png?width=750&format=png&optimize=medium) ### 3D Ready Organoids and Organoid Expansion Services Quality-controlled organoids are manufactured at scale for high-throughput screening, leveraging proprietary bioreactor and bioprocess technology to produce reliable and predictive patient-derived organoids (PDOs). **[Assay-ready organoid vials](https://www.moleculardevices.com/products/3d-biology/3d-ready-organoids "Assay-ready organoid vials")** **[Custom organoid 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Save.")** ![QPix FLEX Picking System](https://www.moleculardevices.com/media_1b945277ebe4a4f75cde698cea83bf199a9a7e7d9.png?width=750&format=png&optimize=medium) ### The QPix® FLEX™ Microbial Colony Picking System for precision, flexibility, and space-limited environments Plating, streaking, picking, and liquid handling, all in one compact device? The QPix FLEX Microbial Colony Picker makes it look easy. With advanced color imaging, it helps you spot the right colonies faster. And yes, it fits on your bench or in a hypoxic chamber – because why wouldn’t it? The QPix FLEX system replaces the clutter with one smart, streamlined system built to move your research forward. **[Learn more](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers#new-qpix-flex-microbial-colony-picking-system "Learn more")** ![Clone Screening Systems for Colony Picking and Single-cell Isolation](https://www.moleculardevices.com/media_18c8d4e78bfcb449b722f08f3c24480a58491736e.png?width=750&format=png&optimize=medium) ### Clone screening systems for colony picking and single-cell isolation The QPix® 400 Microbial Colony Picker, ClonePix® 2 Mammalian Colony Picker, and CloneSelect® FL Imager increase throughput and consistency across cell line development, monoclonal antibody discovery, and synthetic biology workflows. **[Learn more](https://www.moleculardevices.com/products/clone-screening "Learn more")** ![Clone Screening Systems for Colony Picking and Single-cell Isolation](https://www.moleculardevices.com/media_147878830d366fe2bf065f782c473fb377c49c33b.png?width=750&format=png&optimize=medium) ### Clone screening systems for colony picking and single-cell isolation The QPix® 400 Microbial Colony Picker, ClonePix® 2 Mammalian Colony Picker, and CloneSelect® FL Imager increase throughput and consistency across cell line development, monoclonal antibody discovery, and synthetic biology workflows. **[Learn more](https://www.moleculardevices.com/products/clone-screening "Learn more")** ![Automated Solutions for High-Throughput Clone Screening](https://www.moleculardevices.com/media_19de3234d106f597642de7d7b5a49db28ce9d5e52.png?width=750&format=png&optimize=medium) ### Automated solutions for high-throughput clone screening Fully-integrated, lab automation solution for molecular cloning, antibody discovery, and monoclonality. Our automated clone screening workflows integrate laboratory devices to increase your throughput and efficiency while reducing human interaction. **[Learn more](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-clone-screening "Learn more")** ![Clone Screening Systems for Colony Picking and Single-cell Isolation](https://www.moleculardevices.com/media_147878830d366fe2bf065f782c473fb377c49c33b.png?width=750&format=png&optimize=medium) ### Clone screening systems for colony picking and single-cell isolation The QPix® 400 Microbial Colony Picker, ClonePix® 2 Mammalian Colony Picker, and CloneSelect® FL Imager increase throughput and consistency across cell line development, monoclonal antibody discovery, and synthetic biology workflows. **[Learn more](https://www.moleculardevices.com/products/clone-screening "Learn more")** ![Automated Solutions for High-Throughput Clone Screening](https://www.moleculardevices.com/media_19de3234d106f597642de7d7b5a49db28ce9d5e52.png?width=750&format=png&optimize=medium) ### Automated solutions for high-throughput clone screening Fully-integrated, lab automation solution for molecular cloning, antibody discovery, and monoclonality. Our automated clone screening workflows integrate laboratory devices to increase your throughput and efficiency while reducing human interaction. **[Learn more](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-clone-screening "Learn more")** ![Clone Screening Systems for Colony Picking and Single-cell Isolation](https://www.moleculardevices.com/media_147878830d366fe2bf065f782c473fb377c49c33b.png?width=750&format=png&optimize=medium) ### Clone screening systems for colony picking and single-cell isolation The QPix® 400 Microbial Colony Picker, ClonePix® 2 Mammalian Colony Picker, and CloneSelect® FL Imager increase throughput and consistency across cell line development, monoclonal antibody discovery, and synthetic biology workflows. **[Learn more](https://www.moleculardevices.com/products/clone-screening "Learn more")** ![Automated Solutions for High-Throughput Clone Screening](https://www.moleculardevices.com/media_19de3234d106f597642de7d7b5a49db28ce9d5e52.png?width=750&format=png&optimize=medium) ### Automated solutions for high-throughput clone screening Fully-integrated, lab automation solution for molecular cloning, antibody discovery, and monoclonality. Our automated clone screening workflows integrate laboratory devices to increase your throughput and efficiency while reducing human interaction. **[Learn more](https://www.moleculardevices.com/service-support/lab-automation-solutions/lab-automation-for-high-throughput-clone-screening "Learn more")** ## We’re scientists supporting scientists ### Our experts help researchers move beyond traditional testing with complex, human-relevant models—unlocking faster, smarter breakthroughs that truly translate. ![Drug Discovery](https://www.moleculardevices.com/media_121c89801ab5cfefef6b1940e22d67a7209396c23.png?width=750&format=png&optimize=medium) - [Next-gen Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) - [Drug Discovery & Development](https://www.moleculardevices.com/applications/drug-discovery-and-development) - [3D Cell Models](https://www.moleculardevices.com/applications/3d-cell-models) - [Cell Line Development](https://www.moleculardevices.com/applications/cell-line-development) - [Drug Screening](https://www.moleculardevices.com/applications/toxicology) ![3D Biology in Human](https://www.moleculardevices.com/media_1e6a4c3a3ad572f163b545d4e1d7127828007e1f3.png?width=750&format=png&optimize=medium) Next-gen Drug Discovery ### 3D Biology: The paradigm shift in next-gen drug discovery In the midst of an evolving drug discovery paradigm, researchers worldwide are transitioning their compound screens away from 2D cell cultures and animal models to more complex, human-relevant 3D systems like organoids. Experience our interactive infographic as it takes you deeper into why the industry is embracing this next generation of drug discovery and the innovations supporting scientists in their 3D biology journey. _[View infographic](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery "View infographic")_ ![3D Cell Models Drug Discovery](https://www.moleculardevices.com/media_10ae9642ccd6ce212003666b8e0d224095260b5db.png?width=750&format=png&optimize=medium) Drug Discovery & Development ### Drug Discovery & Development The drug discovery landscape is shifting, with more scientists centering cell line development, disease models, and high-throughput screening methods around physiologically-relevant 3D cell models. The reason for this is clear: Using cellular model systems in research that closely mimic patient disease states or human organs can bring life-saving therapeutics to market – faster. _[Advance Drug Discovery](https://www.moleculardevices.com/applications/drug-discovery-and-development "Advance Drug Discovery")_ ![3D Cell Models](https://www.moleculardevices.com/media_1530535824757a2fcf210883885038429083f6d4a.png?width=750&format=png&optimize=medium) 3D Cell Models ### 3D Cell Models With our automated 3D cell culture and bioimaging screening solutions, we are helping reshape the future of drug discovery. Supported by our technology and organoid development protocols, researchers can now advance and scale screening methods for physiologically-relevant 3D models that more closely mimic patient disease states and human organs, leading to faster drug development and approval. _[Spheroids](https://www.moleculardevices.com/applications/3d-cell-models/spheroids "Spheroids")_ _[Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids "Organoids")_ _[Organ-on-a-Chip](https://www.moleculardevices.com/applications/3d-cell-models/organ-on-a-chip "Organ-on-a-Chip")_ ![Cell Line Development](https://www.moleculardevices.com/media_13791897763943f4d403966b83720f0ef48f16d61.png?width=750&format=png&optimize=medium) Cell Line Development ### Cell Line Development Our cell line development customers accelerate time to market for life saving monoclonal antibodies (mAbs) and genomic medicine, as well as perform groundbreaking research in cell and gene therapies, genetic engineering, personalized and precision medicine, synthetic biology, RNA and DNA-based vaccines, and so much more – made possible by our innovative technology and rich expertise. _[Monoclonal Antibodies (mAbs)](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs "Monoclonal Antibodies (mAbs)")_ _[Synthetic Biology](https://www.moleculardevices.com/applications/synthetic-biology "Synthetic Biology")_ _[Cell & Gene Therapy](https://www.moleculardevices.com/applications/gene-editing-with-crispr-engineering "Cell & Gene Therapy")_ ![Drug Screening](https://www.moleculardevices.com/media_1f437c3dc2c6207229f5b4a688029cdd4c7df0532.png?width=750&format=png&optimize=medium) Drug Screening ### Drug Screening For every drug that makes it to the finish line, another nine don’t succeed. This alarming failure rate can be traced to reliance on 2D cell cultures that don’t closely mimic complex human biology, often leading to inaccurate predictions of a drug’s potential and extended drug development timelines. A critical step in the drug discovery process, drug screening and toxicity assessment uncover the effects of potentially life-saving compounds. Shifting to cell-based testing allows multiple chemicals to be tested rapidly and better represents human biology. _[Toxicity Assessment](https://www.moleculardevices.com/applications/toxicology "Toxicity Assessment")_ ## Bridging human ingenuity with smart technology ### [Recent announcements](https://www.moleculardevices.com/newsroom "Recent announcements") showcase collaborations, next-gen technologies, and expert perspectives on industry trends inspiring better science. [![Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/media_121aa45ae22a2c05644f2baea406aa309aca5a5aa.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) [Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/newsroom/in-the-news/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery "Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery") \| Biocompare [![The case for switching from animal models to organoids in preclinical testing](https://www.moleculardevices.com/media_11efc51475821487f157dade3501ef2989d68024f.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/switch-from-animal-models-to-organoids) [The case for switching from animal models to organoids in preclinical testing](https://www.moleculardevices.com/newsroom/in-the-news/switch-from-animal-models-to-organoids "The case for switching from animal models to organoids in preclinical testing") \| Manufacturing Chemist [![3D Organoids vs 2D Cell Lines in Drug Discovery and Disease Modeling](https://www.moleculardevices.com/media_1a2f659184ab6488e3355314df94b549633f53a87.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/3d-organoids-vs-2d-cell-lines-drug-discovery) [3D Organoids vs 2D Cell Lines in Drug Discovery and Disease Modeling](https://www.moleculardevices.com/newsroom/in-the-news/3d-organoids-vs-2d-cell-lines-drug-discovery "3D Organoids vs 2D Cell Lines in Drug Discovery and Disease Modeling") \| Biocompare [![New Preclinical Testing Tools Help Researchers Tailor Treatment but Can They Fully Replace Mice?](https://www.moleculardevices.com/media_11b4dd76d6ef7f8b1341366141a08f5dbbe775880.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/can-preclinical-testing-tools-replace-mice) [New Preclinical Testing Tools Help Researchers Tailor Treatment but Can They Fully Replace Mice?](https://www.moleculardevices.com/newsroom/in-the-news/can-preclinical-testing-tools-replace-mice "New Preclinical Testing Tools Help Researchers Tailor Treatment but Can They Fully Replace Mice?") \| Precision Medicine Online [![Transforming Access to Human-Relevant Models in Complex Biology Workflows](https://www.moleculardevices.com/media_123feb0c5a2f78eab18d755ac77089ba34066f3cc.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/transforming-access-to-human-relevant-models) [Transforming Access to Human-Relevant Models: Automation and Innovation in Complex Biology Workflows](https://www.moleculardevices.com/lab-notes/3d-biology/transforming-access-to-human-relevant-models "Transforming Access to Human-Relevant Models: Automation and Innovation in Complex Biology Workflows") [![Empower Research with Advanced High-Content Screening Technology](https://www.moleculardevices.com/media_1e8de6bcde950183ed16e252b08c25066961a4bdf.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/empowering-researchers-high-content-screening-technology) [Empowering Researchers with Advanced High-Content Screening Technology](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/empowering-researchers-high-content-screening-technology "Empowering Researchers with Advanced High-Content Screening Technology") [![Assay-ready Human Models for Intestinal Biology Research](https://www.moleculardevices.com/media_1ec4c620e12c23d9baef47a6d0576c2b070e71867.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/redefining-research-with-human-relevant-models) [Redefining Research with Assay-Ready Human Relevant Models](https://www.moleculardevices.com/lab-notes/3d-biology/redefining-research-with-human-relevant-models "Redefining Research with Assay-Ready Human Relevant Models") [![The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/media_106121f0c133a1470db5190bd909756473b454fba.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) [The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics "The Transformative Power of AI-Enabled Data Analytics") [![Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/media_121aa45ae22a2c05644f2baea406aa309aca5a5aa.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) [Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/newsroom/in-the-news/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery "Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery") \| Biocompare [![The case for switching from animal models to organoids in preclinical testing](https://www.moleculardevices.com/media_11efc51475821487f157dade3501ef2989d68024f.jpg?width=750&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/switch-from-animal-models-to-organoids) [The case for switching from 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Using [synthetic biology](https://www.moleculardevices.com/applications/synthetic-biology "synthetic biology") techniques like strain engineering, scientists can engineer microbial cells as cellular factories for various organic molecules, most commonly proteins. In the food supply chain, precision fermentation is used to create everything from plant-based proteins from yeast cells to dairy- and animal-free whey protein from fungal strains. For strain engineering to be the most efficient, automation can replace manual colony screening and selection processes in the workflow. Selecting large numbers of high performing microbial colonies is essential to scale up a food product created with precision fermentation. ![Precision fermentation of food](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1f8d86704eff3cab2c767b80092912cdc0d37582f.png?width=750&format=png&optimize=medium) ## Automate synthetic biology workflow for strain engineering Due to the high throughput nature of microbial strain engineering, molecular cloning workflows are prone to human error, labor-intensive, and time consuming. These challenges create large bottlenecks resulting in low productivity. Strain engineering can benefit greatly from the increased productivity of an automated colony picker such as [Molecular Devices QPix® 400 Series Microbial Colony Picker](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers "Molecular Devices QPix® 400 Series Microbial Colony Picker"), which automatically picks up to 3000 colonies per hour or 30,000 colonies per day with automatic pick run data tracking and database management. For higher throughput and more walkaway time, there are custom design options for an automated work-cell that integrates user desired instrumentation, and robotic control with the QPix colony picker to improve productivity and eliminate bottlenecks. ![Automate Synthetic Biology Workflow for Strain Engineering](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1cbfbe6896913a791b68079afdf1a589196a558f3.png?width=750&format=png&optimize=medium) ## Learn more about our research methods Here, we highlight various applications and research methods using our QPIX Microbial Colony Picker and SpectraMax® microplate reader to help scale your synthetic biology workflow. [![Lab Automation 101: Inside Access From Our Subject Matter Expert](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1fce7f853ddc70dcb071ac4772628f1e602b71cd3.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) Blog Nov 02, 2021 ### [Lab Automation 101: Inside Access From Our Subject Matter Expert](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) Get insider insights on lab automation from our subject matter expert.… [Learn more](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) [![Tips for Molecular Cloning & Strain Engineering Efficiency | Molecular 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for the… [Learn more](https://www.moleculardevices.com/en/assets/app-note/br/detect-endotoxin-with-the-kinetic-qcl-kinetic-chromogenic-lal-assay) [![Synthetic Biology Automation: Five Tips to Improve Your Molecular Cloning Process](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1b87dc4f50d709316268c9e7e6844db268948a1b0.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/clone-screening/synthetic-biology-automation-five-tips-to-improve-your-molecular-cloning-process) Blog May 11, 2022 ### [Synthetic Biology Automation: Five Tips to Improve Your Molecular Cloning Process](https://www.moleculardevices.com/lab-notes/clone-screening/synthetic-biology-automation-five-tips-to-improve-your-molecular-cloning-process) Optimize your molecular cloning process with our five essential tips for… [Learn more](https://www.moleculardevices.com/lab-notes/clone-screening/synthetic-biology-automation-five-tips-to-improve-your-molecular-cloning-process) [![Lab Automation 101: Inside Access From Our Subject Matter Expert](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1fce7f853ddc70dcb071ac4772628f1e602b71cd3.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) Blog Nov 02, 2021 ### [Lab Automation 101: Inside Access From Our Subject Matter Expert](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) Get insider insights on lab automation from our subject matter expert.… [Learn more](https://www.moleculardevices.com/lab-notes/general/lab-automation-inside-access-from-our-subject-matter-expert) [![Tips for Molecular Cloning & Strain Engineering Efficiency | Molecular 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Processes](https://www.moleculardevices.com/sites/default/files/2023-10/grow-your-synthetic-biology-processes.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/bpd/grow-your-synthetic-biology-processes) eBook Nov 22, 2023 ### [Grow Your Synthetic Biology Processes](https://www.moleculardevices.com/en/assets/ebook/bpd/grow-your-synthetic-biology-processes) This Synthetic biology E-book explores a fusion of multiple scientific… [Learn more](https://www.moleculardevices.com/en/assets/ebook/bpd/grow-your-synthetic-biology-processes) [![Fermentalg® uses QPix® 420 colony picker to leverage microalgae metabolism to produce natural food dyes](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/media_1595b6a8b2f2a6f9ba9b41d6b1996b62bd906b0fe.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/customer-breakthrough/bpd/fermentalg) Customer Breakthrough Jul 18, 2023 ### [Fermentalg® uses QPix® 420 colony picker to leverage microalgae metabolism to produce natural food dyes](https://www.moleculardevices.com/en/assets/customer-breakthrough/bpd/fermentalg) Fermentalg® studies microalgae for sustainable fish oils, proteins, and… [Learn more](https://www.moleculardevices.com/en/assets/customer-breakthrough/bpd/fermentalg) **[View all Resources](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/precision-fermentation#resources "View all Resources")** ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=precision-fermentation "Speak to a specialist")** ## Resources for Precision Fermentation [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products and Services [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## ClonePix Systems Guide # ClonePixTM Systems APPLICATION GUIDE FOR NEW USERS ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/dd44a6e1a3e85914265cb17013208592cee491568d80db7cacd9cbbeda2449d3.jpg) Contents Table of Figures .... 4 Introduction ..... 6 Setting up mammalian cell line selection experiments. Principles of cell line selection using ClonePix FL ............................... 7 Cell line selection... Semi-solid media .... ...... 9 Selecting cell lines on ClonePix FL ... 11 Monitoring clones post selection. .... 12 Stages of optimization .. 13 1. Understanding the characteristics of the cell line . 13 Cell growth and selection.... ....13 Complexity of the cell population .. 14 2. Defining the objectives of the project .. . 15 3. Semi-solid culture. 15 Seeding cells.... ....15 Addition of fluorescent detection agent... ....15 Monitoring clone outgrowth... ....15 Determining the picking window .. ...15 Determining the optimum density of clones.... .....15 Key points for obtaining good colony growth . 15 4. Imaging Clones on ClonePix FL 15 Filters and exposure times .. 15 Colony detection..... ....15 Groups . 15 5. Optimizing conditions for post-pick outgrowth . 15 6. Defining parameters to find the best clones. 15 Example experiment . ....15 7. Analyzing stability and sub-cloning.... 15 Stability analysis.... ...15 Sub-cloning.. 15 Specific protocols. .15 Reducing timelines for DHFR / Methotrexate selection on ClonePix FL 15 Introduction .... ...15 An alternative process on ClonePix FL .15 Recommended DHFR work flow using ClonePix FL .15 Compatible media. ..15 Protocol guidelines.... ...15 Conclusions... ..15 Using CloneMedia-CHO for selection of DG44 cells.. . 15 Introduction. .15 Materials .15 Methods ... 15 Results ... 15 Conclusions.. .15 Supplementary information. .15 Growth of CHOK1SV in semi-solid media and selection of high expressers......... 15 Introduction .. .15 Methods and Workflow ... .15 Troubleshooting... .15 requently Asked Questions ................. .15 Growing colonies . 15 Fluorescent detection in semi-solid media . 15 Imaging and picking on ClonePix FL 15 Post-pick growth.. 15 Reagents and Supplies... 15 Detection reagents. 15 CloneMatrix. 15 CloneMedia / XPMedia . 15 PetriWell cell culture plates . 15 Other 15 lossary of Terms ...... .15 Contact Details. 15 # Table of Figures Figure 1: Typical workflows for limiting dilution and ClonePix FL.. .. 8 Figure 2: ClonePix FL technology..... .. 9 Figure 3: Detection of high value clones on ClonePix FL . .. 11 Figure 4: Monitoring of the growth of cells in a 96 well plate .. . 12 Figure 5: Correlation between ClonePix FL fluorescence and ELISA productivity with and without confluence correction. ........... ........ 12 Figure 6: Monitoring cell number and viability of a cell population during standard passaging ... . 13 Figure 7: Monitoring cell number of a cell population during overgrow .................. 14 Figure 8: Examples of clones grown in semi-solid media... . 15 Figure 9: Monitoring clone growth.. 15 Figure 10: Colonies detected on ClonePix FL .. ... 15 Figure 11: Verification of good outgrowth after picking on ClonePix FL. ... 15 Figure 12: Composite image from ClonePix FL showing the diversity of colony characteristics ... .. 15 Figure 13: Rank plot showing the \[FITC\] Exterior Mean Intensity values for clones within a batch... .. 15 Figure 14: Productivity results of clones picked by different selection parameters 15 Figure 15: Results of refining criteria to enhance selection of best clones. ... ... 15 Figure 16: Screening for clone instability on ClonePix FL.. .. 15 Figure 17: A mixed cell population of transfected CHO DG44 in HT- selection only. 15 Figure 18: Rank Plot from ClonePix FL software showing the order of detected colonies from high to low fluorescence.. 15 Figure 19: An image of the same cell line shown in Figure 18 showing detected colonies and groups used to analyze colonies............ Figure 20: Images of DG44 colony growth in CloneMedia-CHO at day 6 with or without HT and MTX ... 15 Figure 21: Images of a DG44 non-clonal population of cells forming colonies in CloneMedia-CHO... . 15 Figure 22: A CHOK1SV transfected cell line plated 14 days post transfection in $\\mathsf { \\Pi } \_ { 5 0 \| \| \\boldsymbol { \\mathsf { M } } }$ MSX ... . 15 Figure 23: Workflow for transfected CHOK1SV cells in serum free or chemically defined media.. . 15 Figure 24: A CHOK1SV stable cell line plated into CloneMedia with CloneDetect .... 15 Figure 25: Image of loose or migrating colonies taken with the CloneSelect Imager 15 Figure 26: Fluorescent microscope images of high IgG expressing PER.C6 colonies grown in semi-solid media with CloneDetect.. . 15 Figure 27: Multiplex imaging of hybridoma colonies on ClonePix FL showing minimal bleed-through.. . 15 Figure 28: Graph to show area of the colonies picked and resulting growth rate of the clones.... . 15 # Introduction # Setting up mammalian cell line selection experiments This manual is designed to assist new ClonePix™ systems users and more experienced users set up different applications. There are a broad range of applications which can be performed using ClonePix systems and each user may have slightly different objectives. For this reason it is difficult to compile a complete step by step protocol. Instead, this guide explains the science behind the technology and lays out the key parameters to be aware of when optimizing an experiment or standardizing projects. The experience of Molecular Devices scientists and application specialists has been combined here to give useful tips and techniques to optimize ClonePix systems technology and get the best out of your cells. It is important to understand the material in the first half of the manual before setting up projects. The protocols are general so that they are applicable to different research groups. Understanding the technology will help the user to refine these protocols and tailor them to their own needs. Media-specific, cell-specific and application-specific protocols are presented in the second half of the manual. ClonePix systems and their associated reagents have been used successfully to select clones from a wide range of cell types and applications. This guide is not exhaustive but should be used as a starting point. For each of the products mentioned in this manual, please refer to the instruction leaflet provided with each product for further specific information. Please read the relevant ClonePix FL or ClonePix 2 Software Applications Manual before using either of the ClonePix systems. # Principles of cell line selection using ClonePix Systems The following section is divided into 4 parts: $$ Cell line selection $$ Semi-solid media $$ Selecting cell lines on ClonePix systems $$ Monitoring clones post-selection # Cell line selection When selecting cells from a mixed population it is important to be able to quickly isolate those that are important and discard the rest. Although applications may be very different, there are some general principles which apply in all situations: 1) Screening cells when the population has greatest diversity will give the highest probability of finding the exceptional candidates, and 2) Screening more cells will increase the likelihood of finding rare-event clones up to a limit that is not well-defined and is likely to be different for each parental cell type. Traditional methods for cell screening include limiting dilution and Fluorescence Activated Cell Sorting (FACS). Using limiting dilution, a cell population is diluted down to a statistical value of between 0.1 and 1 cell per well of a 96-well plate. Since not all cells plated will grow successfully, a large number of plates is needed. Each well must be checked by microscope to ensure the presence of only a single cell. Wells with multiple cells cannot be considered clonal. After the cells have grown to a suitable density, all wells containing cells must then be screened for positives and scaled up for further analysis. FACS involves taking a population of cells and incubating with a fluorescent marker e.g. a FITC tagged anti-IgG. Cells are separated and screened via a laser. Positives are directed down one route based on electrostatic charge while negatives are discarded. In principle, the technology works well when the marker to be detected is on the cell surface, but is weaker for secreted proteins. Cells are then typically cloned using one or more rounds of limiting dilution, or individual cells can be collected using a single cell sorter. The physical stresses involved in cell sorting are detrimental to the cells and even an experienced user may expect to loose $5 0 %$ of the cells in the process. ClonePix systems technology bypasses the time and handling constraints of these methods by screening high numbers of colonies of cells in situ, and is particularly effective for secreted proteins. Only clones that are positive for the protein of interest are isolated to a 96 well plate and taken on. It is a much more productive approach and is far quicker in workflow. Figure 1 shows the comparison in workflow between limiting dilution and ClonePix systems. # 1000 clones,2 months ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/ef66608292e63e7fe7547394025012371faac5f04052ec42ffe55df765af33a8.jpg) Figure 1: Typical workflows for limiting dilution and ClonePix systems There is a large difference in the number of clones it is possible to screen between the two methods due to manual labor time, incubator space and cost. The culture method and screening time also has a large bearing on the total time to isolated, high value clones. # Semi-solid media Semi-solid media can be used to grow discrete colonies from single cells, isolated from each other in space but within the same plate. The cells are seeded at low density into a semi-solid medium containing all the components needed for growth. The viscous property of the medium is achieved using methylcellulose, an inert substance derived from plant cellulose. At the correct concentration and molecular complexity, the cells are prevented from movement while nutrients are free to diffuse. The benefit of cloning in semi-solid medium is that each clone is screened as a colony so it has already been shown to grow well, and will have high probability of survival after picking. The process of using semi-solid medium for mammalian cell cloning is well established and was first described in 1982 (Davis, J.M. et al. J. Immun. Methods 50, 161-171). The authors described the method as being “…easier to plate out large numbers of cells and to recover many independent hybridoma clones”. The ClonePix systems technology extends the use of semi-solid media by visualizing and quantifying in situ the specificity or productivity of a target protein produced by the cells. The target protein can be secreted, expressed on the cell surface, or an intrinsically expressed fusion protein (with GFP for example). For secretion assays, the secreted protein is trapped in the vicinity of the colony by a molecule present in excess in the medium that diffuses freely through the medium until it recognizes and complexes with the secreted protein. In the simplest scenario, this molecule is fluorescently conjugated such that the amount of fluorescence accumulating around a colony is proportional to the amount of target protein secreted by the colony. The CloneDetect range of products have been developed and optimized for detection of IgG or other immunoglobulins. An additional benefit of screening fluorescently in semi-solid medium is that the secreted protein is accumulated over time enabling differences between clones to be multiplied over the time, and eliminating cell-cycle effects. This is a much more effective method of determining secretion than measuring a secreted protein at the surface of a single cell as is the case with FACS. After a period of culture at $3 7 ^ { \\circ } \\mathsf C ,$ which can vary from 5-14 days depending on the cell type, clonal colonies can be screened on the ClonePix systems and the positives automatically isolated and transferred back to liquid culture media in a 96-well plate. The process is illustrated in Figure 2. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/8d300f4781d5b58a8e4c076378eaab6e9ec776d01070818eab42cd8761517b9f.jpg) Figure 2: The ClonePix systems technology Molecular Devices semi-solid media products are available in two forms: CloneMediaTM and CloneMatrixTM. These products are based on a form of methylcellulose that is optimal for robust growth of round, suspended clonal colonies, for good formation of the fluorescent secretion complex, and for successful automated picking of clones. The CloneMedia products are complete, ready to use cell line specific semi-solid media. Each bottle contains sufficient for 100mls of medium in 90mls volume thus permitting cells and any preferred supplements to be added directly to the bottle. CloneMedia are designed for maximal cell growth from low cell numbers, and provide ample nutrients for the colonies throughout their culture time. CloneMedia products for CHO cell lines are animal derived component free. CloneMedia products for hybridomas contain fetal bovine serum (FBS) but not hypoxanthine, aminopterin and thymidine (HAT). The XP Media products are liquid versions of their respective CloneMedia products and are designed for optimal cell survival and expansion post-picking. CloneMatrix is a 2.5X concentrated solution of pure methylcellulose for the addition of userdefined liquid medium. Each bottle contains sufficient methylcellulose for 100mls of medium in 40mls volume thus permitting liquid medium and cells to be added directly to the bottle. The liquid medium must be at least 2X concentrated to attain the working concentration after mixing with CloneMatrix and other components. CloneMatrix-CHO is a variant designed specifically for CHO cell lines that contains a chemically-defined supplement essential for good growth in semi-solid media. For full details on the range of reagents and consumables, please see Page 49. # Selecting cell lines on ClonePix systems Batches of up to twelve PetriWell-1 or PetriWell-6 plates with grown colonies are placed into a cassette and then loaded into the source stacker (back left slot). Plates are fed through for imaging automatically under software control. Once the batch has been imaged, the software processes the data and allows the user to create one or more groups of colonies to pick. The software automatically takes into account clonality features such as size, shape and proximity to nearest neighbor. Once a “pick list” is generated by the software, and the cassette of imaged plates has been manually transferred back to the left side of the source stacker (if using ‘Batch mode’), the ClonePix systems automatically pick the identified colonies plate by plate into one or more 96-well destination plates. The illumination system for imaging the plates is non-laser based so there is minimal photobleaching. The picking is carried out under HEPA-filtered laminar flow and the picking pins go through a sanitization cycle after each pick. Only the positive clones are picked and all others are left in the plates. Figure 3 shows images of one area of a plate captured by ClonePix FL where a single clone shows intense fluorescent precipitation around it confirming that it is a high secretor. Only this clone would be automatically picked from this area of the plate. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/90550f7930a2f66ff34d7febb1fc1b41b8b332cc8edccb13903eedd87c561238.jpg) igure 3: Detection of high value clones on ClonePix FL ClonePix FL captures images by white light (left panel) and by fluorescence (right panel). The white light image is used to detect colonies of cells, and the fluorescent image is used to visualize protein of interest secreted from each colony and trapped by the fluorescent detection probe. In this example, CloneDetect™ FITC was used to find highest IgG secretors. # Monitoring clones post selection The optimal size of colonies for picking is between 32 to 200 cells, although the pickable range is much wider. The process of automated picking from semi-solid medium is very gentle such that each colony is transferred with little or no loss of viability. Once the colony is transferred, the ClonePix systems have an option for dispersing the cells so that they are more evenly spread across the well thus encouraging faster growth. The number of picked clones growing out successfully is very much dependent on the composition of the media. In some cases, for example when picking small CHOK1SV colonies, the outgrowth rate may be higher if the colonies are left undispersed. Cells should be monitored for growth post-picking so that a suitable scale up time can be determined, and to eliminate poor growing clones. Molecular Devices recommends CloneSelect™ Imager, a separate imaging system that rapidly scans microplates, records microscope quality images and detects cells to generate a measure of confluence. By capturing images daily, growth rates can be determined for every clone. Figure 4 shows an example of cells picked into a 96-well plate using ClonePix FL. The green overlay is the software detection of the cells. Having the recorded images and analysis takes the manual guesswork out of the equation and means processes can be established for scaling up at a particular confluence, giving more consistency between projects. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/d3adfd4f79cfe13e52e749acc711908cd8cb34f6b8ab6f0fc1e098ccfba94343.jpg) Figure 4: Monitoring of the growth of cells in a 96 well plate During the post-pick phase, it is also recommended that early productivity measurements should be made such as by ELISA. These measurements can easily be corrected for confluence by using the data generated by CloneSelect Imager. Figure 5 shows how data normalized in this way provides a more reliable measure of productivity. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/43acb4ab256c663fdf8918d2aecc55dc8d6800a53b5db8c685ce2724c3fc5f36.jpg) One quarter of the well is shown in the image above, taken from CloneSelect Imager system (shown above). Cells start at day 0, just after picking, evenly dispersed. They start to divide over the next few days to form small clusters which link up to form a confluent well. Figure 5: Correlation between ClonePix FL fluorescence and ELISA productivity with and without confluence correction. Normalizing 96-well stage productivity data for confluence (right panel) shows better correlation than uncorrected data (left panel). # Stages of optimization 1. Understanding the characteristics of the cell line 2. Defining the objectives of the project 3. Semi-solid culture 4. Imaging clones on the ClonePix systems 5. Optimizing conditions for post-pick outgrowth 6. Defining parameters to find the best clones 7. Analyzing stability and sub-cloning # 1\. Understanding the characteristics of the cell line # Cell growth and selection Cell lines will behave differently from each other but there are general characteristics that define each type or sub-type. It is important to be familiar with the specific needs of the cells. If the cell line has been established in other related projects there will probably already be optimized media for the transfection or hybridoma fusion process. Certain supplements may have been evaluated to support good cell growth, survival or high productivity. Most likely, a selective agent will be required to discourage growth of transient expressers or unfused myelomas. Where available, refer to the suppliers instructions on handling the cells and the transfection / fusion process. Semi-solid medium is an inert tool for cloning cells and should not normally require any adaption to the transfection or fusion protocol. The viability and cell number of a fresh transfection, fusion or bulk selected pool should be monitored over a period of 2-3 weeks in liquid culture (see Figure 6). This will demonstrate the effects of the selection agent and the transfection / fusion method on the growth of the population over time. Initially the cell number may drop or remain static as the growth of selected and non-selected cells balances out. Monitoring provides valuable information on how the cells behave just after transfection / fusion as well as during subsequent cloning stages. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/f06957a50870c74ea9a08b90c52920ad6391c3d477625518aeeb89701243b00d.jpg) Figure 6: Monitoring cell number and viability of a cell population during standard passaging Cell counts and viability of an established pool of CHOK1SV cells expressing a therapeutic antibody were taken on consecutive days during a standard passage routine. The cells were grown in an Erlenmeyer flask on a shaking platform at $3 7 ^ { \\circ } \\mathsf C$ , $5 %$ ${ \\mathsf { C O } } \_ { 2 }$ . The growth profile for the same CHOK1SV population during overgrow in an Erlenmeyer flask is shown in Figure 7. Both sets of data provide useful information that can be used to decide the optimal point to seed the cells in semi-solid medium as well as when to pick them. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/960baa9cc21cf6957645256071650733fb732302305bac7ed64e39070a474bb6.jpg) Figure 7: Monitoring cell number of a cell population during overgrow Cell counts taken on consecutive days from an overgrow flask seeded from the same population as in Fig 6 showing the full growth profile. # Complexity of the cell population As well as understanding the effects of cell growth and selective agents, it is important to have knowledge of the transfection / fusion efficiency and the likely complexity of the population. In other words, the expected size of the transfection / fusion population and how many desirable events might be present in the population. This will determine the number of cells that need to be screened to find the required clones. The ClonePix systems can generally screen up to 10,000 colonies in a single run of 8-10 plates in 2 - 2.5 hours, but is very flexible depending on the number of plates that need to be processed. If the transfection or fusion efficiency is low, a high proportion of the cells will be killed or remain static depending on the nature of the selective or metabolic agent applied. A larger number of cells may need to be seeded to obtain an appropriate number of colonies per well (see section 2). This may also affect the way the detection agent is applied since the presence of a large number of dead and dying cells may accumulate the fluorescent detection complex around non-viable cells in the media. Application by atomizer 48h before picking may be advisable in such cases. A low complexity (low number of different genetic events) in the population may mean that screening a large number of colonies may not result in a better clone than would be obtained by screening a small number. For example, if a pool of cells has been bulk selected, e.g. DG44 in a high level of methotrexate without cloning, the cells with low DHFR will die and the population will consist of more genetically similar cells (high redundancy). The same is also true of a cloned cell line which theoretically has no genetic variation and therefore has very low complexity. In reality, established cell lines will gain genetic diversity (complexity) with time, and hence the ClonePix systems can still be used very effectively to re-clone a cell line or even recover a badly deteriorated one with rare high producers within the population. It should not normally, however, be expected that the ClonePix systems will significantly improve a cloned cell line, or find a good clone from a poor transfection or fusion. The technology can only work within the limits of the cell line and elements in the method used. The ClonePix systems technology is most powerful where complexity of the cell population is high and where high value clone frequency is low. # 2\. Defining the objectives of the project Use the following questions as a guide to determining the aims of each project. # How many clones need to be screened? This relates to transfection / fusion efficiency, the number of independent events and whether it is important to screen the whole population or a representative sample. How many clones would you like to have after ClonePix system screening? This may be dependent on throughput of clones in later liquid handling or screening steps. $$ Is the protein you are looking for inside the cell, on the cell surface or secreted? The location of the protein will affect the detection agent used, the time of application and the statistical parameters to be used on the ClonePix systems. # $$ Are you doing a primary screen or subcloning? This may influence the plating size and density as well as the stringency of the colony groups such as exclusion by Proximity. In general, subcloning should be undertaken with highest stringency, e.g. Proximity $\\mathbf { \\bar { \\rho } }$ 1mm. For primary screening many users prefer to plate at higher density and use lower exclusion stringency to screen the largest possible experimental space, with acceptance that a round of subcloning will be necessary to be assured of clonality. # Are you screening for highest producers or antigen specificity or both? This will determine if you need to set up a secretion assay for immunoglobulin quantification (e.g. using CloneDetect) or an antigen-specificity assay for finding antigen positives (tagged / conjugated antigen $^ +$ Complex Initiation Factor (CIF)) or a combination of both to find the best positives (tagged / conjugated antigen $^ +$ CloneDetect). # Do you have an appropriate fluorescent detection agent? CloneDetect fluorescent detection agents are specific to an immunoglobulin or part of an immunoglobulin. It is essential to be sure that you use CloneDetect with the correct species specificity and that the conjugated fluorophore is compatible with the ClonePix systems filter sets that you are licensed to use. For detection of any other secreted protein, it is essential to understand that the detection agent must generate a large precipitation complex with the secreted protein. Simple complexes such as a fluorescent monoclonal antibody probe bound to a maximum of two secreted monomeric protein molecules is not sufficiently complex to trap it in the vicinity of the secreting colony, so it will diffuse through the semi-solid medium. A monomeric secreted protein can be probed with a polyclonal or a mix of two or more complementary monoclonals, while a multimeric protein should be detectable with one monoclonal probe. Care should be taken if it is necessary to use a fluorescent secondary probe, i.e. when the primary probe cannot be fluorescently conjugated, as the two may precipitate together in the background of the semi-solid medium. Ideally therefore, secondary probes should be monovalent such as Fab fragments. # Do you need to multiplex more than one fluorophore? ClonePix systems can multiplex using a maximum of 3 different fluorescent channels: 1. CFP or FITC/EGFP 2. Rhodamine 3. Cy5 Examples where more than one fluorophore may be required are: 1. Antigen $^ +$ immunoglobulin for antigen-specificity assay 2. Detection of multiple antigens after immunizations with multiple antigens 3. Quantification of IgG production and a post-translational modification # Are any other factors, such as growth important? The size of the clonal colony is recorded on the ClonePix systems. The downstream use of the clone may determine the relative growth rate required to work well. For example, a transfected clone being used for large scale manufacture of a biotherapeutic molecule will need to grow at a reasonable rate without growing too slowly and not producing enough protein or too fast and causing a faster decline of the viability of the population. Although growth rate is not measured directly, the size of the clonal colony is a good indication. # 3\. Semi-solid culture Seeding cells In initial experiments, an established transfected or hybridoma cell line should be used to practice growing cells in the appropriate CloneMedia. A higher seeding density will be needed for a non-selected population at an earlier stage after transfection or fusion but these initial experiments will confirm that the media conditions are correct and give the operator some experience in handling the media. Cells should be seeded into CloneMedia or CloneMatrix-based media as they enter exponential growth – usually 48 hours post split. If the cells cannot not be split because the whole population must be kept together, fresh liquid medium should be added 24 – 48 hours before. A freshly transfected population should generally be left for 48 hours to recover before counting and seeding into semi-solid media, although some users prefer to bulk-select the population for a week or more before seeding. Hybridoma fusions should be left to recover for at least 18-24 hours. It is crucial to determine the optimized seeding density empirically for each cell line. The following provides starting guidelines: Stable serum free cell line: 250-500cells/ml Stable serum containing cell line: 50-200cells/ml Serum free transfection: 1000-2500cells/ml Serum containing transfection: 500-2000cells/ml Hybridoma Fusion: 105 – 106 cells/ml During the initial stages of optimization, a variety of cell densities should be set up spanning at least the range recommended above. CloneMedia should be used because it is a complete ready-to-use semi-solid medium, and there are different compositions optimized for specific cell types. As an alternative, CloneMatrix concentrate can be used with a high quality cloning media that will support low cell numbers and continued growth. This may mean mixing media or adding single cell cloning supplements. Refer to the relevant product booklets for dedicated handling instructions. In initial experiments, it is a good idea to test CloneMatrix with a variety of media compositions as well as CloneMedia to rapidly ascertain what optimal conditions for your cell type. CloneMedia and CloneMatrix are conveniently provided in bottles that provide an appropriate amount for one screen. If using less than a whole bottle of CloneMatrix, any common components should be added to the bottle to decrease the viscosity prior to aliquoting. Alternatively, CloneMatrix can be aliquoted using a sterile 10ml syringe to insure maximum transfer of the viscous concentrated product. # Addition of fluorescent detection agent # At time of plating The CloneDetect fluorescent detection agents are optimized for use on the ClonePix systems and are designed for ease of use. In most cases, any detection agents such as CloneDetect or a tagged/conjugated antigen should be added to the semi-solid medium immediately prior to adding the cells. This requires minimal processing and gives the best signal to background ratio because all components have maximum time to diffuse through the medium and generate the fluorescent precipitation complexes around the colonies. # By atomizer An alternative method for addition of detection agent is via an atomizer at least 48 hours before picking. As this requires an extra manual plate handling step, it is only recommended in a few select situations where: 1) transient expressers are abundant in the semi-solid medium. These tend to form small, very bright fluorescent precipitation complexes and can give a false positive signal, even if they are no longer viable by the time picking is initiated; 2) the detection agent is likely to deteriorate during the incubation period such as a labile antigen; and 3) a number of experimental media conditions or plating conditions are being established. It may be advisable to wait until the conditions for successful growth are identified before adding detection agent to confirm expression of the target protein. # Monitoring clone outgrowth The plating of cells to semi-solid medium is effectively the single cell cloning stage where each cell is held away from all others. For this reason, cells disturbed during the very early adaption and growth phase may fail to form colonies. After plating out, the plates should ideally be placed to the back of an incubator that is not used for everyday cell culture work as this can cause significant vibration and fluctuation in ${ \\mathsf { C O } } \_ { 2 }$ levels. The plates should be left undisturbed for at least four days. If it is necessary to observe colonies as they grow, a monitoring plate can be set aside from a batch of identical plates that can be imaged without disturbing the rest of the plates. By day 5, hybridoma colonies should be starting to become evident against the fusion background. For cells grown in chemically defined media, the cells may have only just started dividing (i.e. expect the initial growth rate to be slower than in liquid culture). If none of the cells has started dividing at this stage, it is unlikely that they will start. In this case, the experiment should be repeated trying new media conditions or higher seeding density. If advice is needed, contact Customer Support. When transferring plates always be sure to carry them flat – DO NOT tilt. Avoid touching the base of the plates as much as possible as this may affect image quality. For most cell types grown in chemically defined media, the colonies will not be visible to the naked eye until day 7. The quickest way to check for growth is to hold a plate up to the light and look from below. Colonies should be visible as small dots of white in the clear media. If there are fluorescent reagents in the media, avoid overexposure to the light (more than a few minutes). If plates need to be placed on the bench for any length of time it is advisable to place a sheet of aluminum foil over them to protect the fluorophore from photo-degradation. Figure 8 shows examples of clones grown in semi-solid media. Further examples are available in the CloneMatrix and CloneMedia protocol guides. Repeated imaging of one plate from a batch allows monitoring of colony growth and physiology. Figure 9 shows an example of where tracking of clone growth is useful. It can be used to show continued growth of colonies and helps determine the optimum time for picking or with media optimization – for example if cells start to divide but growth arrests or the majority of cells fail to divide. The appropriate changes can be made to the media formulation to correct for this. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/cb9b328370c611d5b641d2a0cf864e440a8b40fb6a65c80be0148ee5f24b0d89.jpg) Figure 8: Examples of clones grown in semi-solid media A: CHO DG44 cell line in chemically defined media, day 9. B: Freshly transfected CHOK1SV nonselected cell population at day 10 showing small clones growing from a background of debris. The debris is a result of the selective agent. C: An IgG producing clone from a mouse hybridoma fusion after 10days in semi-solid medium. The precipitation complex between CloneDetect and the secreted IgG is just visible around the clone by white light. Images captured on CloneSelect Imager. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/3b1a6735ee6002a400b51318ec62c125250ada2369f44556e9b8090676b88c1d.jpg) Figure 9: Monitoring clone growth A high expressing cell line $( > 2 9 / 1 ~ \\mathrm { I g G } )$ was plated into chemically defined conditions in semi-solid medium. The cells were not large enough to pick at day 12 (A) so were left for another four days (B) by which time growth had picked up significantly and clones still appeared healthy. The cell line continued to grow well. It was discarded at day 20. Images captured on CloneSelect Imager. # Determining the picking window The optimal size of colonies for picking in terms of growth and productivity is 32 - 256 cells (5-9 cell divisions). The ideal day for picking will vary depending on the cell line used and it’s doubling time which may be different in semi-solid medium compared to liquid culture. Within a population there may be very small colonies (less than 32 cells) and very large colonies (greater than 256 cells). Very small or very large colonies may be undesirable to pick because of their extrapolated growth rates. A colony of less than 32 cells at the day of picking may have growth issues as a clone and may struggle to adapt to liquid media afterwards or be too slow to be of practical use. A very large colony greater than 500 cells may turn necrotic in the centre and is probably dividing too fast to be producing the protein of interest in sufficient quantity. The following is a guideline for the best picking window based on experience at Molecular Devices: Hybridoma/ transfected myeloma stable cell lines in serum: day 5-7 Hybridoma fusion: day 7-10 CHO DG44/ CHO-S in serum: day 7-10 CHO DG44/ CHO-S serum free or chemically defined: day 9-12 CHOK1SV chemically defined, serum free or serum containing: day 14-16 CHO fresh transfectants: day 12-16 PerC6 serum free: day 12-14 Other cell types should be judged based on the above guidelines and observations of size and quality of the clonal colonies. In general, serum-containing cultures will grow quicker than serum-free cultures, which will grow quicker than chemically defined cultures. Transfected cells will grow slower than their non-transfected parents. Very high levels of expression may result in a lower growth rate. Cell lines which are designed to grow in culture at high density and with high expression may need a higher level of supplementation to allow them to be cultured from single cells in the semi-solid media. They may undergo a lag period without division when first seeded into semi-solid media and again once picked, before adjusting to their normal growth rate. # Determining the optimum density of clones The optimum number of clones per well depends on the assay and cell type. A balance is needed between screening the appropriate number of colonies and sufficient confidence of clonality. For fresh transfections or hybridoma fusion screens, it may be necessary to screen a very large number of clones in order to screen the whole population at once. One strategy would be to seed a high density of cells to generate greater than 200 clones in a well of a PetriWell-6 plate or 1000 in a PetriWell-1 plate. To pick rare high expressers or antigen positive cells, it will be necessary to drop the Proximity group cut-off to $0 . 4 \\mathsf { m m }$ or less, and the Irregular group parameters (Roundness & Axis Ratio) to 0.3-0.5. As the probability of clonality is reduced in this situation, the best clones may need to be re-cloned. Where clonality must be achieved in a one-step process only, colony number should not exceed 50 per well in a PetriWell-6 plate or 250 in a PetriWell-1 plate. Proximity should be kept at 1mm and Irregular kept at 0.6 or greater to give a high degree of confidence in clonality. There is no benefit of setting Proximity higher than 1mm. Another factor to consider is the morphology of clones formed in semi-solid media, which is cell type dependent. Cells in chemically defined or serum-free conditions tend to be small and compact so can be plated at higher density. It is possible to have up to 200 clones per well of a 6-well plate without a significant loss of pickable clones to proximity parameters. Cells such as hybridomas and some adherent CHO may form large colonies of loosely packed cells and so may need to be plated at lower density such as 50 clones per well. Initial experiments plating cells at different densities can easily be analyzed using the Imaging Run process on the ClonePix systems (see below) to determine how many colonies fall into the Accept and Proximity groups for each seeding density. Seeding densities of future experiments can then be adjusted accordingly. # Key points for obtaining good colony growth $$ Ensure viability is high $( > 9 5 % )$ and that cells are in the exponential phase of growth. The health of the cells and the stage of their growth prior to plating in the semi-solid media is the key to reliable colony formation. With most cells, passaging ${ \\sim } 4 8$ hrs prior to plating will give the best results, though this will vary with different cell types and growth conditions. If cells are not healthy before plating, they will not be healthy after plating! $$ Bring all media components to room temperature before plating. Do not heat/thaw to $3 7 ^ { \\circ } C$ . It is essential that CloneMatrix/CloneMedia is not heated above $3 0 ^ { \\circ } \\mathsf { C }$ prior to plating. Heating above this affects the physical properties of the media. CloneMatrix/CloneMedia will thaw at room temperature in ${ \\sim } 3$ hours. $$ Mix CloneMatrix $+ \ 2 x$ media (or CloneMedia) and add any supplements required. Add ddH2O, if required, to bring the final volume up to $1 0 0 ~ \\mathsf { m l }$ . Shake bottle to mix. Add $1 %$ Clone Detect. Mix by rolling/inverting bottle. Add the appropriate amount of cells. Mix gently but thoroughly (it is best to turn the bottle upside down a few times). Leave to stand for $\\mathord { \\sim } 5$ minutes to allow any large bubbles to rise to the surface. This makes plating far easier and far more efficient. Plate as need be ( $\\cdot \\sim 2 \\mathsf { m l }$ /well of a 6 well plate or $\\sim 9 \\mathsf { m } \\mathsf { l }$ l/well of a 1 well plate). It’s easiest to use a $\\mathsf { 1 0 m l }$ or $2 5 ~ \\mathsf { m l }$ pipette for this (draw up an extra $2 \\mathsf { m } \|$ into the pipette in order to minimize any air bubbles getting into the wells). Tilt the plate to distribute the media. Pop any large air bubbles with a sterile pipette tip or move them to the edge. Add a couple of mls of sterile ddH2O or PBS to the outside reservoirs. Without this, the semi-solid media will dry out and the cells will not survive. Incubate undisturbed for at least 4 days. # 4\. Imaging Clones on ClonePix systems New users should refer to the relevant ClonePix system Quick Set-Up Instructions for an overview of how to use ClonePix FL or ClonePix 2, and to the Software Applications Manual for detailed instructions. To ensure meaningful statistics are obtained so that the best clones are picked, it is vital to determine and apply the correct imaging and picking parameters. The Imaging Run process allows you to image a plate or a batch without committing to a picking run. The aim of this is to determine the range of sizes of colonies and determine the ideal picking day. It should save time on the day of picking because the optimal fluorescence and size settings will already be defined. # Filters and exposure times The correct filters and exposure times need to be set up in the Acquisition tab, and should always include a White Light (Trans) setting. Exposure time and LED intensity should be set low for white light – approximately 150ms and LED intensity of 3. The image should display the colonies clearly without overexposure and saturation of the pixels (indicated by patches of red on the image). The LED intensity for a fluorescent wavelength should normally be set at the maximum of 128. The exposure time should generally be between 500-2000ms. For very high expressers it can be lowered for better discrimination between the clones. For cases where the protein of interest is expressed at low levels, exposure can be raised to 10 seconds, although his may significantly increase the run time. A control plate with a cell line not reactive to the fluorescent detection agent should be used to determine the level of cellular auto fluorescence. # Colony detection Once the acquisition options are set, the colonies need to be detected by selecting a Colony Detection Algorithm and the appropriate Average Colony Diameter in the Detection tab. Getting the correct detection settings prior to a picking run is critical to achieving good results. It is imperative that the software detects all colonies (otherwise good ones may be missed) but that the software is not mistaking non-cell background particles as colonies as this will limit the number of colonies that can be picked due to proximity. There are four different algorithms for determining the outline of the colonies. These are Global Threshold, Local Threshold, Edge Detect and Flatten Detection. Full descriptions of each of these can be found in the relevant ClonePix system Software Applications Manual. The most appropriate algorithm for optimal colony detection is dependent on cell type, colony growth, media/plates used, and the application. For well defined, clear and discrete colonies with a clean background, Global or Local Threshold options are likely to give the best results. Edge Detect is highly sensitive and primarily designed for detection of adherent monolayers. To set up the Average Colony Diameter, move the slide bar on the Detection tab until you find the setting that best covers your range of colony sizes. Expect this to be toward the left side of the slide bar. An average colony diameter of $0 . 3 \\mathsf { m m }$ is normal for CHO colonies (Figure 10) and 0.5mm for larger colonies such as hybridomas. If using Edge Detect, the Average Colony Diameter setting may have to be set higher to eliminate detection of non-cell background objects. The diameter of individual colonies can be measured using the ruler icon in the bottom left of the screen. If the plate shows large numbers of single cells or small, failed colonies, the detection should not include these. These cells should not be able to grow on so should not present a problem with clonality. If in doubt, the best clones can be put through a second round of screening in semi-solid media. Always ensure that plates are kept clean and smudge free. Clean the underside of plates with $70 %$ ethanol on a non-abrasive cloth or wipe before imaging if required. If using serum, ensure it is filtered before plating in order to remove precipitates. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/f57a7280aced2c30f24015f2193e136a4686f170524e9f7c99f3fb1a947fa6c8.jpg) Figure 10: Colonies detected on ClonePix FL The colonies in this test experiment were quite small. Local threshold with an average colony diameter of $0 . 3 \\mathsf { m m }$ gave the best fit. # Groups Once the whole plate or batch has been imaged and the colonies automatically detected, the Groups tab can be set up. Getting the correct settings for groups allows the user to ensure the resulting clones are monoclonal (by using the settings for colony shape). In addition, it enables the user to influence the growth rates of the resulting clones (by using the settings for colony size). Perhaps most importantly, the user can ensure that only the very highest expressing colonies are picked (by using the fluorescent settings and colony size settings). The Rank Plot and Scatter Plot can be used to analyze different statistic features across the population, aiding the set-up of groups to include or exclude certain types of colonies. The default group values provide a good starting point: # Too big This parameter excludes unwanted objects (e.g. bubbles) and colonies that are too big (as colonies growing too quickly are unlikely to be high producers). Keep this set to the default value of $\\mathsf { \\bar { \\rho } } > 0 . 7 \\mathsf { \\bar { m } } \\mathsf { m } ^ { 2 }$ . # Too small The recommended minimum colony area is $0 . 1 ~ \\mathsf { m m } ^ { 2 }$ but it is feasible to go as low as 0.05 $\\mathsf { m m } ^ { 2 }$ with minimal loss, or even $0 . { \\dot { 0 } } 3 { \\mathsf { m m } } ^ { 2 }$ with some loss of outgrowth success. Lower settings will result in very poor outgrowth as the limited number of cells will be unlikely to survive the low plating density. A small colony is also indicative of a slow growth rate. # Irregular 1 - Compactness This measures the compactness of a colony. A perfectly circular colony would have a value of 1. A colony with a perimeter twice as long as it would be if it were a perfect circle would have a value of 0.5, and so on. # Irregular 2 - Axis Ratio This measures the ratio of the axes of a colony. A perfectly circular colony would have a value of 1. A colony with the shortest axis half the length of the longest axis would have a value of 0.5, and so on. In both case, a value of less than 0.6 is not advisable as picked colonies are likely to have derived from more than 1 cell, and will therefore not result in a monoclonal population. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/de4160bd155a6da59b1639c312d424569d2d78daff1b9bee16a84ade7460bd05.jpg) # Proximity How close the colony is to its nearest neighbor. 1mm ( ${ \\sim } 3 0$ pixels) is considered a safe setting for clonality work. This may be reduced down to 0.8 or $0 . 7 \\mathsf { m m }$ without significant increase in risk, or can be reduced substantially to 0.3 or $0 . 2 \\mathsf { m m }$ if the user intends to re-clone. # 5\. Optimizing conditions for post-pick outgrowth Optimal cell growth after picking is key to the overall success of the cloning experiment. Molecular Devices’ range of XP Media liquid expansion media are specifically designed for this purpose and provide continuity in formulation when used in conjunction with the respective CloneMedia semi-solid media product. Superior outgrowth rates will generally be achieved if picked clones are initially collected into XP Media and then moved to your preferred growth medium than to pick directly into the growth medium. If you choose to develop your own post-pick outgrowth media, it should be formulated to support low cell numbers. It is recommended to pre-test a number of different outgrowth media variants with cells seeded at low density (50-200 cells/well), and select the medium that provides healthy cells with the best outgrowth rate. A good outgrowth medium should reach $> 7 0 %$ confluence within 14 days as measured by CloneSelect Imager. Cells may undergo some stress as a consequence of being moved to liquid conditions from a semi-solid environment, but the picking of a colony of clonal cells greatly improves the chances of successful outgrowth. Other ways to maximize the outgrowth success rate are 1) pick colonies into the destination well without dispersal so that they remain in close proximity to each other for the first few days, and 2) start with a low initial volume in the destination well (for example ${ \\bf 1 0 0 \\mu l \\updownarrow }$ ) to increase the rate that the cells condition the medium, and then supplement with further medium after a few days. Where selective pressure is high it may be beneficial to lower the concentration of the selective agent or remove it completely for at least the first few days after picking. A representative sample of clones should be picked from the test plates into the chosen outgrowth media to verify survival. If survival is low, the media may require additional supplementation or the colonies may need to be grown further before picking. CloneSelect Imager allows monitoring of growth post-picking and the confluence data may be used to normalize early ELISA data (Figure 11). ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/d5d30111bd1574fef39e02593c9619be466c7def453ab547fbfd73fbadceccc1.jpg) Figure 11: Verification of good outgrowth after picking on ClonePix FL Example of good outgrowth of clones picked from a transfected CHO-S population under serum free conditions. Confluence measurements and growth curves produced on CloneSelect Imager. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/4423480e9d76f98dce19a136cc578012cc134ec3401c80517ef8b96e5d9c6cd0.jpg) # 6\. Defining parameters to find the best clones A small improvement in productivity can make a huge difference to cost when it comes to producing a biotherapeutic on an industrial scale. Colonies grown in semi-solid medium from a heterogeneous population will often show diverse physical characteristics (Figure 12). Understanding what physical characteristics identify a clone as a good producer enables ClonePix FL to work most efficiently at picking the best. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/031237e1fb5e899a48ca88ebca5add7476f6d8f9231173f8352172b2c1443a28.jpg) Figure 12: Composite image from ClonePix FL showing the diversity of colony characteristics A transfected CHO cell population showing a variety of clones with different size and expression profiles. Using the statistical features on ClonePix FL, these can be divided into separate groups for exclusion or picking. Once good semi-solid media conditions have been established, it is recommended that an experiment be carried out to determine the parameters that will best identify your high value clones. A large number of identical test plates should be set up so that each parameter can be analyzed separately. Four plates for each parameter should be sufficient to provide good data confidence, although fewer can be used for a small test experiment. Each batch should be picked from separately, keeping all settings identical except the parameter used to identify the best clones. The parameters most likely to successfully and efficiently detect the highest producers are the fluorescence measurements of Sum Total Intensity, Exterior Mean Intensity, Interior Mean Intensity and Normalized Intensity. In the software they will be prefixed by the filter used e.g. \[FITC\] Exterior Mean Intensity. # Example experiment # Aim To take a mixed population of transfected CHO cells and identify the best parameters to select the highest expressers. # Materials $$ A bulk selected population of IgG-secreting CHO DG44 cells selected in 250nM methotrexate $$ CloneMedia-CHO (K8710) $$ CloneDetect FITC anti-human (K8200) $$ 0.1mM stock Methotrexate $$ Dialyzed FBS to a final volume of $5 %$ $$ XP Media-CHO (K8750) $$ PetriWell-1 plates non-TC treated (W1055) $$ PetriWell-96 plates non-TC treated (W1555) # Method A mixed population of DG44 cells was seeded into CloneMedia to give $2 0 \\times 1$ -well plates. CloneDetect was added to the media and cells at Day 0. At Day 10, the plates were divided into 4 batches to be picked using the 4 main fluorescence parameters. $$ Group A: Exterior Mean Fluorescence Intensity $$ Group B: Normalized Fluorescence Intensity $$ Group C: Interior Mean Fluorescence Intensity $$ Group D: Sum Total Fluorescence Intensity Figure 13 shows the Rank Plot for \[FITC\] Exterior Mean Intensity. The threshold was set so that any clone with medium or high Exterior Mean Intensity was included in the Accept group. A group was set up so that any clone with low Exterior Mean Intensity was gated off. Only cells which passes the clonality aspects of the groups filter, and which were over a threshold value of fluorescence were picked. The same was done for each of the other batches. 96 clones (or as close to that as possible without picking clones of low fluorescence) were picked from each batch. These were then grown in 96 well plates for 7 days while monitoring for growth using CloneSelect Imager. The media was then changed and the cells left for another 5 days before supernatant samples were taken for IgG quantification by ELISA. ELISA values were compared across the groups to determine which statistical feature gave the best clones. The best clones were then compared back to the fluorescence values to determine where they were placed in the ranking and to look for correlation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/e1b1ef40d229b88b16acaf3cfe147de659225f29f72d12b5d28acaadfd5ca4e0.jpg) Figure 13: Rank plot showing the \[FITC\] Exterior Mean Intensity values for clones within a batch The threshold should not be set below the level where the curve flattens out if picking for high expression. The low Exterior Mean Intensity clones (green) are excluded from picking by adding another group to discriminate between clones. In the example above, any clones with a FITC exterior mean intensity value lower than 100 would not form part of the Accept group and so would not be picked. # Results The results of the experiment are shown in Figure 14. In this example \[FITC\] Exterior Mean Intensity gave the best result with Sum Total Intensity a close second. Figure 14: Productivity results of clones picked by different selection parameters | | | | | | --- | --- | --- | --- | | | n | Average productivity (ug/ml) | Productivity corrected to 10o% confluence (ug/ml) | | A: Exterior Mean | 57 | 34.91 | 54.28 | | B: Normalized | 22 | 23.22 | 35.31 | | C: Interior Mean | 23 | 21.05 | 29.33 | | D: Sum Total | 40 | 24.80 | 47.79 | ${ \\mathfrak { n } } =$ number of clones grown successfully post-picking. Only clones showing growth were used in the average productivity calculation. Productivity corrected for $1 0 0 %$ confluence uses the percentage confluence measured at the time of supernatant harvest. IgG concentration measured by ELISA was divided by the confluence and multiplied by 100. In most cases this is only required minor adjustments to normalize the data for cell number. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/6aa9f32cecf800e4dd5b1f1d687e3f372dfe0faa685748d530378f360a6a2ff6.jpg) Average Productivity of Groups A-D # Refining the criteria In order to refine the criteria for selecting the best clones, the data for the high FITC Exterior Mean Intensity group was re-analyzed by removing data points below 400U, increasing the minimum colony size to $0 . 0 5 \\mathsf { m m } ^ { 2 }$ and decreasing the maximum size to $0 . 2 \\mathsf { m m } ^ { 2 }$ then $\\bar { 0 . 1 } \\mathsf { m m } ^ { 2 }$ . The results of this re-analysis are shown in Figure 15. Tightening the fluorescence and size criteria together doubled the average productivity of the included clones. | | | | | | --- | --- | --- | --- | | | n | Average productivity (ug/ml) | Productivity corrected to 100% confluence (μg/ml) | | Original Group A | 57 | 34.00 | 54.3 | | <0.2mm2 | 32 | 55.25 | 86.3 | | <0.1mm2 | 20 | 78.45 | 124.6 | ${ \\mathfrak { n } } =$ number of clones grown successfully post-picking that fell into the specified cut-off group. Second group criteria: \[FITC\] exterior mean ${ < } 4 0 0 \\cup$ and colonies $\\mathsf { \\bar { 0 } } . 0 5 \\mathsf { m m } ^ { 2 }$ to $0 . 2 \\mathsf { m m } ^ { 2 }$ . Third group criteria: \[FITC\] exterior mean ${ < } 4 0 0 \\cup$ and colonies $0 . 0 5 \\mathsf { m m } ^ { 2 }$ to $0 . 1 \\mathsf { m m } ^ { 2 }$ . ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/64034be630e14cefbc11269014ee2743cd53e6ca0e123f598ecf25579fdeeaae.jpg) Figure 15: Results of refining criteria to enhance selection of best clones. # Conclusions and application to other projects In this example, Exterior Mean Intensity was found to be the statistical feature which best selected the clones with highest productivity. Figure 11 showed that only clones with very low fluorescence had been excluded from picking. Refining the criteria to analyze the group with fluorescence over 400U instead of the threshold of 100U set in the original experiment eliminated a number of low productivity clones. When analyzing the data, it was clear that the size of the clone was a factor in predicting productivity and that the larger clones are undesirable. Large clones can give a falsely high value for Exterior Mean Intensity because it will be producing a large quantity of antibody overall (giving a high mean intensity reading immediately around the clone) even if the same is not true on a per cell basis. The two refinements of the picking criteria eliminated large colonies by taking the maximum size down from $0 . 7 \\mathsf { m m } ^ { 2 }$ to $0 . 2 \\bar { \\mathsf { m m } } ^ { 2 }$ then $0 . 1 \\mathsf { m m } ^ { 2 }$ . The decrease of maximum size from $0 . 2 \\mathsf { m m } ^ { 2 }$ to $0 . 1 \\mathsf { m m } ^ { 2 }$ notably improved prediction of the better-producing clones showing that, with this data set, clones larger than $0 . 1 \\mathsf { m m } ^ { 2 }$ were not high producers. The results are not entirely unexpected because this cell line is secreting the IgG in large quantities beyond the colony perimeter so an exterior measurement of fluorescence would be expected to be predictive of productivity. The high productivity levels of the best clones in this population suggest that the higher secretors do not have sufficient resources for high growth rate, and conversely that fast growers are not going to be the highest producers. Selecting clones by Sum Total Intensity was the second-best predictor of high productivity. This feature takes the total fluorescence associated with the colony and its exterior area into account. It might be expected that Sum Total Intensity will not be as strong a predictor as Exterior Mean Intensity because a large colony with a high intensity fluorescent area would be ranked superior to a small colony with a high intensity fluorescent area, whereas by Exterior Mean Intensity the effect of the colony is excluded. Interior Mean Intensity is primarily designed for measuring fluorescence closely associated with the colony (e.g. internal GFP expression or probe detection of surface proteins) and so takes no account of secretion. It would thus not be expected to be as predictive where secretion from colonies is evident. Normalized Intensity takes the Sum Total Intensity value and corrects for the size of the colony. This might be expected to be the best predictor of specific production rate but unfortunately tends to bias towards the smallest clones (for further information please refer to Appendix C of the Software Manual). In some cases, users have reported that Normalized Intensity can be a good predictor so long as minimum colony size is kept high enough to reduce the effect of the bias. The above example only provides guidelines on how to test for best parameters for your cells in your working environment. With any cell line it is important to consider the location of the fluorescence and apply this knowledge to determine which of the features should be predictive of productivity. Intracellular or cell-surface fluorescence may be best represented by Interior Mean Intensity. Isolating hybridomas from fusions where expression of IgG may be low and there may be much background interference will most likely be best isolated by Sum Total Intensity. Re-analysis of statistical data generated by the ClonePix systems can provide vital information for improving the result of a picking run. # 7\. Analyzing stability and sub-cloning Stability analysis Ensuring genetic stability in a clonal population of cells is a crucial aspect in cell line selection. A cell line in which a percentage of the cells become unstable will show a drop in production rate leading to a potentially serious loss of product generation. The quest to find the highest possible producers means that clones are often selected that are inputting so much energy into production that growth rate is limited. As a consequence, if a single cell mutates such that it bypasses production of the protein of interest, it can then grow substantially faster leading to a rapid loss of productivity as the mutant strain takes over the population. Therefore, it is important to verify stability of high producing cell lines soon after initial selection and advisable to repeat this at multiple points through the expansion process. The ClonePix systems provide a simple and powerful way to screen for early instability by allowing the user to observe daughter clones within a cell line that are failing to secrete the protein of interest (Figure 16). # Method Label up sufficient PetriWell-6 plates for the number of clones to be analyzed for stability. Up to 60 can be handled fairly easily in a batch. Dilute appropriate CloneMedia to final working volume including the fluorescent detection probe of interest. For CloneDetect use $1 0 0 \\mu \\mathrm { l }$ per 10mls of CloneMedia. Mix thoroughly by gentle rotation. Do not add cells. Pipette 2mls per well into PetriWell-6 plates. Pipette approximately 500-2000 cells from the first clone into the CloneMedia in the first well while stirring around, and then keep stirring to spread the cells. A typical transfer volume would be $2 0 \\AA \\mathrm { - } 5 0 \\mu \\mathrm { l }$ . Repeat for all other clones. Incubate for 7 days. Inspect for stability by carrying out an Imaging Run on the ClonePix system. For each well, a large number of small clones in close proximity should be observed (note: proximity is unimportant here as this is simply an offline visual check). The best way to check the stability of each clone is to toggle between white light and fluorescent images, and any that are not stable should be obvious. There may be minor differences in size and fluorescence but there should not be any low or non-expressers in the population. Any cell lines that show heterogeneity should be discarded. However, some users have reported that re-cloning unstable clones by picking high producing daughter clones can recover cell lines and make them stable. Unst ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/e51ba2c0836fe1fe0404ca67bc30297fd025cb88929849ca09bad8375eaf48fe.jpg) Figure 16: Screening for clone instability on ClonePix FL A stable clone (left panel) is identified by all daughter clones showing fluorescence and all at a similar level. An unstable clone (right panel) is identified by a heterogeneous mix of fluorescence levels. In this case, about a third of the daughter clones are not expressing at all. # Sub-cloning In a similar manner to the above stability analysis, the ClonePix systems provide a robust way to do sub-cloning or cell line rescue. This method is very effective and widely used. Because discrete clones will be picked, it is important that the cell population is plated at lower density, and that the distribution of cells is optimal. It requires more handling than the stability analysis method. # Method $$ Label up sufficient PetriWell-6 plates for the number of best candidates clones to be subcloned. A maximum of 24 clones is suggested and it is best to assign 2 wells to each clone. $$ Dilute appropriate CloneMedia to final working volume including the fluorescent detection probe of interest. For CloneDetect use $1 0 0 \\mu \\mathrm { l }$ per 10mls of CloneMedia. $$ Mix thoroughly by gentle rotation. Do not add cells. $$ Aliquot the CloneMedia into $1 5 \\mathsf { m l }$ tubes with $4 - 5 m =$ in each. $$ Determine viable cell counts for each clone. $$ To the $1 5 \\mathsf { m l }$ tubes, pipette an appropriate number of cells from each clone to generate $\\sim 5 0$ colonies per well. The number required will depend on your cell type. For guidelines, aim for the lower end of recommended seeding densities (see page 15). $$ Mix tubes well to distribute cells evenly. $$ Pipette 2mls per well into PetriWell-6 plates using a $\\mathsf { 1 0 m l }$ disposable pipette. Some users prefer to pipette 1ml to the middle of the well and then pipette a further 1ml of CloneMedia $^ +$ CloneDetect without cells around the perimeter to maintain the cells in the center of the well. $$ Incubate for the required culture time (see page 18). $$ Pick sub-clones using the ClonePix systems. # Specific protocols # Reducing timelines for DHFR / Methotrexate selection on ClonePix FL # Introduction Dihydrofolate reductase (DHFR) catalyses the reduction of folate to tetrahydrofolate and so is part of the biosynthesis pathway for purine, thymidylate and glycine. The cell lines CHO DG44 and DUXB11 have mutant alleles of dhfr and cannot survive in the absence of hypoxanthine and thymidine (HT) which enable use of a salvage pathway. Cells are transfected with dhfr and the gene of interest (on 1 or 2 co-transfected vectors), and selected for ability to grow in HT minus (HT-) media. If two vectors are co-transfected, a second selectable marker such as neomycin resistance is normally used. Cells may be cloned out at this stage and analyzed for productivity of the protein of interest, or they may be subject to methotrexate (MTX) selection to increase productivity. MTX is a folate analogue which binds DHFR. The concentration of the drug is increased in a stepwise manner to encourage amplification of the dhfr locus in response. Only cells which produce DHFR at a high enough level will survive. A large region of the surrounding DNA is amplified at the same time, so the gene of interest should also be present at high copy number. At each round of amplification, it is necessary to split the population into separate flasks and expose to varying concentrations of MTX. The flask which shows about $10 %$ survival will be selected, stabilized, split and exposed to the next range of concentrations. Attaining the maximum level of expression may require raising the level of MTX up to $2 \\mu \ M$ . Reports suggest that there is no benefit from using greater concentrations. The resulting cell lines can show very high levels of productivity in the multiple grams per liter range. The whole process of amplification and selection routinely takes 6 months. There is a high cost in terms of timescales and manual input. # An alternative process on ClonePix FL After selection in HT- media, the population is diverse in expression of DHFR and the protein of interest. ClonePix FL can be used at this stage to screen and pick out those clones with highest expression of the protein of interest. Figure 17 shows that a minority of clones demonstrate high secretion at this stage, which may represent an early increase in copy number and thus a potentially higher propensity for amplification. If higher expression is needed the best picked clones can be exposed directly to high levels of MTX, thus shortcutting the process of selection. This can be done using separate clones, or by pooling the best clones together prior to MTX escalation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/f36458f99e35a461db21b77693e5d4df9004bf4bebfddd7916901ad63d52fdce.jpg) Figure 17: A mixed cell population of transfected CHO DG44 in HT- selection only DG44 cells were transfected to express human IgG and then bulk selected in the absence of hypoxanthine and thymidine (HT-) prior to being plated in CloneMedia-CHO under HT- conditions in the presence of CloneDetect FITC anti-human IgG (B). The high expressing clones are circled in red on image A. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/a362466b0c3bb45cb4366ddee1777dc9686310dd418147df8000fc547fee3d1d.jpg) Recommended DHFR work flow using ClonePix FL # Compatible media The following products are recommended for DHFR / MTX selection. $$ Semi-solid media: CloneMedia-CHO (K8710) $$ Expansion media: XP Media-CHO (K8750) $$ Methylcellulose concentrate: CloneMatrix optimized for CHO (K8530) $$ Antibody detection: CloneDetect anti-human IgG (K8200) CloneMedia-CHO and XP Media-CHO have been shown to support good growth of dhfr $^ +$ cells but not dhfr- cells. All media products are chemically defined but can be supplemented with serum if required. Refer to the product documentation for specific instructions on use. # Protocol guidelines Selection of DG44 cells by ClonePix FL is most effective early in the process during HTselection or at low MTX levels. The power of ClonePix FL at high levels of MTX should be expected to be much less due to the reduced heterogeneity of secretion in the population. However, it may be useful to analyze the stability of the highly amplified population or to reclone using ClonePix FL. Cells must be left for a short recovery period post-transfection to improve viability and to allow the initial selection of transfected cells to take place. 48 hours is sufficient. When plating cells within the first two weeks after transfection, a high seeding density is required. 1000-2000cells/ml is sufficient. A mixed population established for more than two weeks without MTX selection or in low concentrations (up to 500nM MTX) can be plated at 350-500 cells/ml. A cell line or mixed population in high MTX (greater than $\\mathsf { 1 } \\mu \\mathsf { M }$ ) will be under significant pressure and so the seeding density will need to be increased up to 2000 cells/ml. Supplementation such as glutamine (if making up own media from CloneMatrix, CloneMedia products contain 8mM glutamine), insulin or other supplements may help cell viability. The recommended density for viable, established (at least four cell divisions) colonies is 100-200 per well of a 6-well plate or 500-1000 clones per 1-well plate. Incubate the cells in semi-solid media for 10-12 days. Image and pick cells on ClonePix FL, selecting the top $5 \\text{‰}$ of expressers based on high Exterior Mean Fluorescence Intensity or Sum Total Fluorescence Intensity. Colonies should be between 0.05 and $0 . 2 \\mathsf { m m } ^ { 2 }$ for the best result. Monitor the outgrowth of colonies and do an ELISA screen after 7-10 days when the clones have reached $4 0 \\mathrm { - } 7 0 %$ confluence. If using CloneSelect Imager, the growth rate and final confluence may be taken into account when assessing the highest producers on a per cell basis. Once the best clones are established, they may be scaled up and characterized or pooled and amplified. Rejecting the poor expressers early on in the process will leave an enriched pool of higher quality clones to amplify where expression levels are already good. Stability analysis can be done at any stage to assess the variability within the population. A few thousand cells from a clone are seeded into an aliquot of semi-solid media in one or more wells in a 6-well plate. The plates should be incubated for 7 days and analyzed using ClonePix FL software. The rank plot and scatter plot can be used to view the relevant statistical features of fluorescence and size. The Rank Plot should show a slight slope to the left hand side, a leveling off and then a slightly steeper curve (S-shape). Figure 18 shows a cell line demonstrating this effect. If there is a very steep drop off from the left of the graph and a significant number of low producers then the cell line is not stable or clonal. It can be discarded in favor of better clones or re-cloned. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/de70ca68b39582b204c1c297e54b99f0f4a3ee5fd53a2f06856166036d9e9a85.jpg) # Figure 18: Rank Plot from ClonePix FL software showing the order of detected colonies from high to low fluorescence Figure 19 shows a fluorescent image of the same cell line. Although some clones show a greater exterior fluorescence area the intensity of the clones is very similar. In this case small differences are due to colony size. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/df3ef78d08a65cc4632e58619885cd7655e9ea4c07897b05772a4e76f5df0f6b.jpg) Figure 19: An image of the same cell line shown in Figure 18 showing detected colonies and groups used to analyze colonies The image was captured at 500ms FITC exposure. # Conclusions Production of therapeutic or research antibodies in DG44 cells by MTX amplification can result in very high levels of protein of interest but requires up to 6 months to complete the process. ClonePix FL offers an alternative process of early screening to identify and pick medium to high expressing clones that are better candidates for rapid amplification. Both the time line and quality of the cell lines produced can be improved using this method. # Using CloneMedia-CHO for selection of DG44 cells # Introduction The cell lines CHO DG44 and DUXB11 have mutant alleles of the dhfr gene and cannot survive in the absence of hypoxanthine, thymidine and glycine which enable use of a salvage pathway. CloneMedia-CHO is a complete semi-solid media product compatible with a broad range of CHO cell types. The optimal composition of CloneMedia-CHO means that it is free of hypoxanthine or glycine, but contains a trace amount of thymidine at a concentration 8-fold lower than that necessary for rescue of dhfr-- cell lines. The equivalent liquid media for cell line expansion, XP Media-CHO, also contains the trace amount of thymidine. The semi-solid concentrate for CHO cells, CloneMatrix-CHO, is provided with a vial of CloneXL that contains a $5 0 \\times$ concentrate of thymidine to provide the same working concentration in final CloneMatrixbased media. The following study investigates the whether this level of thymidine is sufficient to support growth of dhfr- cells, and if this is affected by the presence of methotrexate (MTX). # Materials $$ CHO/dhfr- cells (ATCC CRL-9096) $$ CloneMedia-CHO (K8710) $$ Dialyzed FBS (dFBS) (Gibco 26400-044) $$ MTX (Sigma M9929) $$ 100x Hypoxanthine, Thymidine (HT) solution (Sigma 11067-030) $$ PetriWell-6 plates, non TC treated (W1155) # Methods CHO DG44 dhfr- cells were grown in static culture in alpha MEM media minus nucleosides, $5 %$ dFBS and 1xHT solution. Cells were trypsinized, counted and seeded into aliquots of CloneMedia-CHO supplemented with $5 %$ dFBS at 500cells/ml. The following conditions were set up: A. No HT or MTX B. 1x HT C. 100nM MTX D. 100nM MTX and 1x HT All reagents were brought to room temperature before mixing, and the cells were $9 8 %$ viable before plating. Each condition was prepared to a volume of 15mls in a $1 5 \\mathsf { m l }$ Falcon tube, and then one 6-well plate was plated at 2mls per well for each condition. Plated cells were incubated undisturbed for 4 days and then imaged at days 4, 5 and 6 on CloneSelect Imager. # Results Images of colony growth for each condition at day 6 are shown in Figure 20. The results show that CloneMedia-CHO without HT supplementation is not sufficient to support the growth of dhfr- cells (A and C). The addition of 1X HT is required to rescue the cells whether in the absence or presence of 100nM MTX (B and D). B. 1x HT ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/24cedddfef290f06c21730aa1b61eb0be3275f88f84e52e2c0e312f21675e079.jpg) A. No HT or MTX # Figure 20: Images of DG44 colony growth in CloneMedia-CHO at day 6 with or without HT and MTX Colony growth was evident only in the presence of $\\mathsf { 1 } \\times \\mathsf { H T }$ irrespective of the absence or presence of MTX. A Single cells are visible but no cell division can be seen. B Compact colonies of about 32 cells are clearly visible and cells are showing expected level of growth. C Only single cells can be seen with no evidence of cell division. D Cells have undergone at least 5 cell divisions and are looking very healthy. Figure 21 shows good selective growth of a non-clonal pool of DG44 cells under MTX selection in CloneMedia-CHO. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/e7368b2d5a2586b5c3b69f942f94328b4c3dc5f81e64627bf10c36200c0ec6f4.jpg) Figure 21: Images of a DG44 non-clonal population of cells forming colonies in CloneMedia-CHO Cells expressing a human IgG were incubated with FITC anti-human CloneDetect (K8200). The image on the left is a 90ms white light exposure taken on ClonePix FL. The image on the right is of the same population taken with a 500ms FITC exposure, showing the comparative expression levels of the colonies. # Conclusions The cells had a high viability before plating and all the plates were identical except for the supplementation with HT and/or the selection agent, MTX. The seeding density used in the experiment was 500 cells/ml $\_ { \\cdot = 1 0 0 0 }$ cells/well). This is about twice as high as would be needed for a cloning experiment and the colonies can be seen quite close to each other in the conditions that grew well. A high seeding density was necessary to increase the number of cells screened in this experiment and give the cells every chance of growing through. In a dhfr- cell line, the cells will not be able to use the primary pathway for purine and pyrimidine biosynthesis involving reduction of folate to tetrahydrofolate. The alternative salvage pathway minimally requires the presence of hypoxanthine and thymidine. The addition of MTX should not additionally affect dhfr- cell lines because they are already using the salvage pathway but it will suppress any dhfr $^ +$ revertants. In this study, there were no revertants, as evidenced by the lack of any colony growth in CloneMedia-CHO only (condition A). This study demonstrates that the level of thymidine at 8-fold lower than the amount required for mutation rescue is not sufficient to support growth of dhfr- cells. CloneMedia-CHO and XP Media-CHO are therefore suitable for selection of dhfr $^ +$ cells whether in the absence or presence of MTX. # Supplementary information Levels of thymidine, hypoxanthine and glycine required to rescue and culture dhfr- cells: $$ Hypoxanthine 6.8mg/L (0.05mM) $$ Glycine 20mg/L (0.26mM) $$ Thymidine 1.94mg/L (0.008mM) Final working concentrations in CloneMedia-CHO and XP Media-CHO: $$ Hypoxanthine nil $$ Glycine nil $$ Thymidine 0.24mg/L (0.0010mM) # Growth of CHOK1SV in semi-solid media and selection of high expressers # Introduction CHO cells use glutamine synthase (GS) to catalyze the synthesis of glutamine from glutamate and ammonia. Methionine sulfoximine (MSX) blocks endogenous GS activity, allowing MSX to be used as a selectable marker. A plasmid containing GS and the gene of interest is transfected into CHOK1SV cells and the population is then selected for growth in glutamine free media. The CHOK1SV line is adapted for good growth and expression at high density in a bioreactor but this makes them difficult to clone as single cells. Cloning of transfected cells is traditionally done using limiting dilution at 4000 cells/well in a 96 well plate. Because of low transfection efficiency and survivability of the cells, only about 15 clones will grow across each plate. ClonePix FL bypasses this by screening the whole population of cells after the initial effect of the selective agent. This gives better cloning efficiency and fewer transients. The stability of these cells can be an issue. ClonePix FL allows re-screening of cells within three weeks of isolation. A few thousand cells are plated back into a small volume (2ml) of semi-solid media and analyzed for divergence of the population. # Methods and Workflow The guidelines given here are specifically for CHOK1SV cells in serum free or chemically defined media and under MSX selection. # Transfected cells Transfected cells are grown and bulk selected in media containing 25 or $5 0 \\mu \ M$ MSX for 14 days prior to seeding into semi-solid media. Cells should be kept in static culture at about $2 { \\times } \\dot { 1 } 0 ^ { 5 }$ viable cells/ml in Gibco CD CHO or other similar media as recommended by Lonza. Experiments at Molecular Devices have shown that the total cell number changes little during this period as non-transfected cells die and are replaced by the expanding transfected population. At day 14, cell viability will start to pick up and this is the best time to plate cells. Cells should be seeded into a $\\mathsf { 1 0 0 m l }$ bottle of CloneMedia CHOK1SV (K8725) at 2000 cells/ml, although optimal plating density will be dependent on the transfection efficiency and cell handling practices of each laboratory and may need to be determined empirically. MSX can be supplemented into the semi-solid media but if cell growth problems are encountered it may be necessary to not add selective pressure during the semi-solid media phase. By separating the bulk selected cells from each other in semi-solid media and then screening for high antibody expression, the most productive clonal colonies can be isolated efficiently without the need for MSX. The isolated clones can be returned to MSX selection shortly after picking. CloneDetect anti-human detection agent (K8200) can be added to the semi-solid media at the point of cell plating. However, to prevent transient expressers that remain viable after bulk selection from being visualized as IgG-positive clones, it is recommended that CloneDetect should be sprayed on via atomizer applicator (K8201) ten days after plating. At day 14, the clones should be imaged on ClonePix FL (Figure 22). # White light 90ms exposure ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/a41af6a14a1e0bce308d9f61007e9fa455252aa3d6e9d714ddc4c1d454ab6b0e.jpg) Figure 22: A CHOK1SV transfected cell line plated 14 days post transfection in $5 0 \\mu m$ MSX ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/f16a06329e1599f30a79dc3a92e4ca9e9301bde410801c5f73dde99128d44391.jpg) FITC 500ms exposure CHOK1SV cells were transfected to express a human IgG, bulk selected for 14 days in MSX and then plated to CloneMedia-CHOK1SV. CloneDetect anti-human in an atomizer applicator (K8201) was added by spraying on at day 10 to avoid detecting transient expressers. Images taken on ClonePix FL at day 14. Only clones larger than $0 . 0 7 \\mathsf { m m } ^ { 2 }$ and with a high Exterior Mean Intensity should be picked into 96-well plates containing $1 5 0 \\mu \\mu$ of standard culture media (recommend Gibco CD-CHO) without MSX. This will maximize the chances of clones growing out. The isolated clones can be supplemented with MSX after 48 hours. From this point onwards use standard cell culture procedures. Only transfer clones up when the confluence is greater than $40 %$ to maintain good viability. CHOK1SV cells grow best at high density. Figure 23 shows a workflow outline for transfected CHOK1SV cells in serum free or chemically defined media. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/89b03097dda2581296ef8be3c98a477267faae82431a2f8e5c6db37c033cb200.jpg) Figure 23: Workflow for transfected CHOK1SV cells in serum free or chemically defined media # Established cell lines Established cell lines should be cultured in Gibco CD CHO or similar media as per standard protocol. 48 hours after passage, cells should be plated at 500-1000 cells/ml into CloneMedia-CHOK1SV. The protocol for transfected cells can then be followed with the exception that cells can be seeded in the presence of CloneDetect (K8200) since there is no risk of detecting false positives from transient expressers. Figure 24 shows a stable high expressing CHOK1SV cell line seeded into CloneMedia with CloneDetect. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/c9290a7aff4aec9c0ff31238a99b63cec32ee1fd2b4b98bd819baa50b984b164.jpg) Figure 24: A CHOK1SV stable cell line plated into CloneMedia with CloneDetect A human IgG expressing CHOK1SV cell line selected on ClonePix FL and grown to 24 well stage was reseeded into CloneMedia-CHOK1SV to assess clone stability. CloneDetect anti-human (K8200) was added prior to plating. Images taken on ClonePix FL at day 10. # Stability analysis and sub-cloning of high expressers Once clones are established in 24 well plates, the highest expressers can be counted and a small quantity re-seeded into semi-solid media. It is sufficient to seed into one well of a 6- well plate. Use densities and conditions recommended for a stable cell line. Between days 7- 10 depending on growth, the cells can be re-screened on ClonePix FL. The clones should be detected on ClonePix FL using the Local Threshold and 0.2mm Average Colony Diameter settings. Data can be analyzed on ClonePix FL or exported for further analysis. Even within a highly cloned cell line there will be some variation in growth rate and productivity. A cell line with a normal distribution of fluorescence intensities as detected by ClonePix FL can be considered stable. If the distribution is skewed towards low fluorescing clones then the cell line may have diversified. In this case, the cell line in question should recloned to see if it can be recovered. The unstable phenotype does not appear to be heritable. # Troubleshooting Plating conditions should be established with a high viability stable cell line. Several practice attempts at plating are advised if the user is unfamiliar with semi-solid media. CHOK1SV cells grow slowly in semi-solid media. After 4 days, small clusters of 2-8 cells should be expected. If no growth is seen in the plates at this time, the plating conditions are not sufficient for cell growth. The plating should be repeated after reviewing plating conditions. Ensure the plates are kept at high humidity by adding sterile PBS or water to the reservoirs around the wells of the PetriWell-6 PetrWell-1 plates. If the incubator used is not giving high enough humidity then cells may need to be isolated in a sterilized plastic container within the incubator with a cover placed loosely over the top. The two time points when the cells are at low density in their immediate environment are when initially adding to semi-solid media and immediately after picking in the 96 well plate. The semi-solid media is supplemented with chemically defined components to support low cell numbers. If problems with growth are seen post-picking, MSX could be removed from the post-pick media until cell number is higher. Alternatively the volume of the media could be dropped from $1 5 0 \\mu \\mu$ to 100 or $7 5 \\mu \\mu$ . This speeds up the rate of conditioning of the medium by the cells. Pre-conditioned media up to $10 %$ can also be used if available. Poor viability can be an issue when transferring cells from semi-solid media to 96-well plates in the same way as it can when transferring cells at each scale up stage. This is not directly due to the transfer method but is mostly due to CHOK1SV not coping well with low cell density. This effect can be minimized by transferring larger cell numbers – colonies larger than $0 . 0 7 \\mathsf { m m } ^ { 2 }$ in semi-solid media and wells above $40 %$ during routine cell culture. # Frequently Asked Questions # Growing colonies Semi-solid media # Can cells be cultured for over 14 days in semi-solid media? Culture periods of 14 days would be considered uncommonly long. Any decline in colony viability is NOT specific to semi-solid media. Beyond this length of incubation, media is likely to show signs of nutrient depletion and desiccation, both of which will affect colony viability. Furthermore, any very large colonies may start showing signs of necrosis in the colony centre due to insufficient diffusion of oxygen and nutrients. This will hamper downstream steps of colony outgrowth and cell line expansion after picking. # Can agarose or our own methylcellulose be used as an alternative to CloneMatrix? The use of agarose is not recommended as an alternative to methylcellulose as this does not give the best conditions for growing colonies of mammalian cells or formation of the secretion complex. ClonePix FL is able to collect agarose plugs but this is primarily for its application in picking plaques of baculovirus-infected insect cells. CloneMatrix has been formulated such that the concentration and chain length of the methylcellulose is optimal for formation and automated picking of mammalian cell clones. Meticulous optimization will need to be performed if using your own methylcellulose. # The CloneMedia protocol does not recommend a serum concentration more than 1%. Why is this? What is the risk of increasing serum concentration in CloneMedia? It is possible to use concentrations of serum over $1 %$ but this may decrease the number of pickable colonies. Innately adherent cell types such as CHO, even after adaption to serum free conditions, will display varying degrees of adherence when grown in the presence of serum, and this becomes more of an issue at higher serum concentrations. The ideal scenario is for cells to form suspended colonies or tightly packed adherent colonies. High levels of serum may result in more dispersed colonies, making them harder to detect on ClonePix FL. Dispersed colonies also require more space and thus need to be seeded at lower seeding densities to ensure monoclonality. # Colonies are not tightly-packed and the cells have migrated away from the bulk of the colony. How can this be avoided? A case of loose or migrating colonies is shown in Figure 25. This is most likely to occur with adherent cells particularly when the serum concentration is high (see above), if plates have been moved, or if the wrong plate type has been used. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/0c51280a5c13db4aebad7bc719019dcc904c3acc7a7472fa01aa8d41080d36ef.jpg) Figure 25: Image of loose or migrating colonies taken with the CloneSelect Imager To maximize the formation of tightly-packed colonies: $$ Reduce the serum concentration where using serum. $$ Leave plates undisturbed for at least 4 days post-plating. This includes frequent opening of the incubator door. $$ Do not use tissue culture treated plates as this will encourage colonies to grow along the plate surface. Use non-TC treated plates such as PetriWell-1 (W1055) or PetriWell-6 (W1155). $$ The compactness of the colonies can be increased by reducing the seeding density. At very high seeding densities, cells may migrate towards conditioning growth factors produced by nearby cells. The timing of imaging and picking in situations such as this can be crucial. Colonies that show this behavior often grow as discrete colonies, before abruptly disintegrating. It is therefore worth monitoring the growth of colonies (e.g. repeated imaging of a test plate on CloneSelect Imager) to determine the best time to pick. It is preferable to pick earlier using a smaller Average Colony Diameter than to pick less compact colonies. Dispersed colonies (such as those in the diagram above) are difficult to accurately image, define and group and are therefore unlikely to produce monoclonal populations. Some users have reported that their particular cell type benefits from being plated to a different medium viscosity. Modulation of the methylcellulose concentration should only be necessary in very exceptional circumstances. Contact discovery [support@moldev.com](mailto:support@moldev.com) for advice. # Why is the number of colony outgrowths so low? Even with high measured viability at plating, not all cells will form colonies. This is primarily because each cell is effectively being plated singly in semi-solid medium. Cell populations that have recently undergone a major treatment such as hybridoma fusion or transfection will need to be plated at high seeding density. Newly transfected cells (4-10 days post transfection) can be plated as high as $1 \\dot { \\times } 1 0 ^ { 6 } \\mathsf { c e l l s / m l }$ . When starting optimization with a new cell type, it is advisable to test a range of cell densities. A second consideration is the composition of the semi-solid medium. The CloneMedia range provides a good base for many cells but it may be necessary to add supplements such as those that that your cells may need in liquid conditions. A third possibility is if the plated have been moved or subjected to vibration or knocks within the first few days. The cells are particularly sensitive at this time. # Why does the semi-solid medium appear cloudy as if contaminated, despite no other indicators of contamination? The secretion complex formed between CloneDetect and the secreted protein is often mistaken for contamination. When viewed by eye or by brightfield microscopy, the precipitation complex can appear as a cloud around each colony, or as a speckled mass of tiny spots. This is normal. If viewed with a fluorescent microscope, the secretion complex can be seen to fluoresce (Figure 26). ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/a99785238f50f08dc13654c5497455f92cfe50c92cc17bfbf98db234169785c3.jpg) Figure 26: Fluorescent microscope images of high IgG expressing PER.C6 colonies grown in semi-solid media with CloneDetect In addition, some turbidity of the semi-solid media itself is normal. FBS will often give a cloudy appearance to semi-solid media and should be filtered before use. Media turbidity can be minimized by bringing all components of the semi-solid media to room temperature before mixing. If any components are cold they may cause precipitation of the methylcellulose and this will not re-dissolve once plated. This will give a cloudy appearance and won’t encourage cell growth. It is important that the semi-solid medium is thawed at $4 ^ { \\circ } C$ or room temperature and is not heated. # Why has the semi-solid medium dried out during incubation? Assess the humidity control capacity of the incubator being used. Incubators that are continually accessed may have a limited capacity to maintain humidity. In such cases, it can be beneficial to store the plates in sealable boxes (such as sandwich boxes) in order to maintain humidity locally. This is more important for colonies that require $^ { 1 2 + }$ days to form. Please contact [discovery.support@moldev.com](mailto:discovery.support@moldev.com) for assistance. Check that sterile $H \_ { 2 } O$ or PBS is added to the PetriWell plate reservoirs when plating. # CloneXL CloneXL is an integral component of CloneMedia-CHO and XP Media-CHO, and is provided as a $5 0 \\times$ concentrate in a small vial with each bottle of CloneMatrix-CHO. It is a chemically defined supplement designed to enhance cell growth at low seeding densities. It is used predominantly with CHO cells in serum free or chemically defined media. If you have serum in the media or a cell line which grows well anyway the difference plus and minus will be minimal. CloneXL is suitable for use with all CHO selection systems e.g. glutamine synthase, DHFR selection, G418 etc. # What is CloneXL? A $5 0 \\times$ concentrate of amino acids and nucleotides. # A precipitate is visible on thawing - is this normal? Formation of a precipitate on thawing is normal and does not affect product quality. However, it may affect product efficacy in semi-solid culture and so must be completely resuspended. Any precipitate occurring during defrost should dissolve upon mixing at room temperature. Re-suspension can be aided by aspirating and dispensing the product several times with a 1ml pipette. # Fluorescent detection in semi-solid media CloneDetect # CloneDetect protocol states the concentration in Units/ml. What is the actual concentration in mg/ml? The CloneDetect protein amount varies between products with a typical concentration of $7 5 0 \\mu \ g / \\mho !$ (for a $7 . 5 \\mu \ g / \ m \\up$ final concentration). The exact concentration is specified on the Certificate of Analysis (CofA) issued with each batch. # Should I modulate the concentration of CloneDetect to match the expected secretion rate? The recommended CloneDetect working concentration of 100 Units/ml ( $1 0 \\mu \\mu$ per ml of semisolid media) has been extensively optimized for a many cell types with a wide range of expression levels and should not need adjusting. Modulation of the CloneDetect concentration should only be necessary in very exceptional circumstances. Please contact [discovery.support@moldev.com](mailto:discovery.support@moldev.com) for assistance. # Will the CloneDetect signal be affected if the cells are repeatedly brought out of the incubator? It is good practice to ensure that plates are covered (with aluminum foil or similar) if they are to be exposed to bright light for longer than a few minutes. It is recommended that lights in tissue culture hoods are turned off when handling CloneDetect. # What is CIF? Complex Initiation Factor. CIF is unconjugated CloneDetect. The primary use is where it is desirable to create a precipitation complex around each IgG-secreting clone but without visualizing by fluorescence. For example, when also probing with FITC-conjugated antigen so that any fluorescent colony must be an antigen-specific IgG secretor. A CloneDetect with a different fluorescent wavelength to the antigen could equally be used. A case where CIF would be obligatory would be where 3 different antigens are probed simultaneously with 3 different fluorophores thus using all available multiplex channels. # Probing with antigen # What antigen concentration should be added to hybridomas plated in semi-solid medium? A suitable working concentration for antigens is $5 \| \| { \\mathfrak { g } } / { \\mathfrak { m } } \|$ . If the antigen is small, it is advisable to reduce the mass to maintain a good molar ratio otherwise there is a risk of a high background of fluorescent antigen which may mask the weak positive colonies. Recommended antigen concentrations are: $ 5 0 - 1 5 0 ~ \\mathsf { k D a }$ proteins: 5 to $1 0 ~ \\mu { \\sf g } / \\mathsf { m l }$ $ ~ 1 0 { - } 5 0 ~ \\mathsf { k D a }$ proteins: 1 to $5 ~ { \\mu \ g } / { \ m \\mu }$ $ \ 2 - 3 \ \\mathsf { k D a }$ peptides: 0.1 to $0 . 2 ~ \| \\mathsf { u g / m l }$ Antigen adsorption: If peptides are small, it might be worth considering conjugating them to a carrier protein. Some customers have found that this helps to prevent non-specific adsorption of the fluorescent antigen to proteins in the medium. # How can I get my antigen probe fluorescently conjugated? There are many commercially available kits for conjugation of proteins and antibodies. Prior to using on cells in semi-solid medium, it is important to validate that the conjugated antigen is still active, i.e. comparable with unconjugated antigen. Molecular Devices only offers a conjugation service for large scale studies. # Can I avoid fluorescently conjugating my antigen probe? The best scenario is always for the primary probe to be directly conjugated. However, it is possible to use a fluorescent secondary probe but it is essential to validate beforehand that there will be no cross-reaction that would complex the primary and secondary probes together in the medium thus stopping them migrating to the positive clones. # Imaging and picking on ClonePix FL Multiplexing # Can multiple fluorescent probes be used in the same experiment? Yes. ClonePix FL has 5 fluorescent filter sets. Up to three of these sets can be used simultaneously with minimal bleed-through. These are: $$ CFP or EGFP/FITC $$ RHODAMINE/DS-RED $$ Cy5 # Will a strong FITC signal interfere with a weak rhodamine label? The EGFP/FITC and RHODAMINE/DS-RED filter sets are discrete channels. However, very strong FITC fluorescence can show some bleed-through into the rhodamine channel. Rhodamine fluorescence has not been observed to bleed into the FITC channel. A “true” rhodamine signal should be clearly distinguishable from a “false” signal leaking through from the FITC channel (see Figure 27). Adjustments to the exposure times and LED intensities (in the Acquisition tab) should be made until the best images are obtained. If one probe is expected to show a stronger signal than the others, then it is advisable for it to be conjugated with the fluorophore with longer wavelength. For example, CloneDetect549 or CloneDetect649 should be used in conjunction with a FITC-conjugated antigen. ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/75c9e9ab3810f86c725d7e765294a9b63a730314a91e52447469987e8de245e1.jpg) # Figure 27: Multiplex imaging of hybridoma colonies on ClonePix FL showing minima bleed-through Two Hybridoma populations: one expressing IgG1, the other expressing IgG2A. Colonies were grown in semi-sold media containing AlexaFluor488 anti-Ig $\\mathsf { G } \_ { 1 }$ (visible through the FITC filter) and AlexaFluor546 anti- $\\cdot \\mathsf { I } \\mathsf { \\mathsf { g } } \\mathsf { G } \_ { 1 }$ (visible through the rhodamine filter). Plates were imaged with white light, FITC 300 ms exposure and rhodamine 2000 ms exposure. # Autofluorescence # Colony fluorescence matches the shape of my colonies. Is this really secreted protein? This is likely to be cellular autofluorescence. It is not an issue for small, healthy colonies but can become significant when colonies are large ( $5 0 0 +$ cells), and especially when cells are dying or dead. When colonies are small it is reasonable to assume there is no autofluorescence (e.g. when using Fluorescent Sum Total Intensity). However, when colonies are large, it is best to use ‘External’ parameters to exclude any contribution by autofluorescence (e.g. Exterior Mean Intensity). The main characteristic of autofluorescence is that it appears as the same shape as the colony, whereas fluorescence related to secreted product is more diffuse and often much larger than the colony. If high autofluorescence is observed, another option may be to run ClonePix FL assays at an earlier timepoint (e.g. day 10 rather than 12). This may improve the quantification of the halo (making it smaller and brighter) as well as substantially reducing autofluorescence. If possible, use CloneDetect 649, as cells show less autofluorescence at longer wavelengths. # Post-pick growth # The survival of cells after picking is very low. Is this normal? Under optimal conditions, ${ > } 9 0 %$ of the picked clones are expected to survive and grow after picking. The physical act of picking the cells does not harm them. It is low cell density that is the primary cause of failure of clones to grow out. For particularly delicate cells, the dispersal settings may be reduced or not used at all. If the survival of cells after picking is low, it can be due to one or more of the following: $$ Poor selection parameters (e.g. picking clones that are too small). $$ Unsuitable outgrowth media $$ Incorrect picking settings (e.g. pin height, dispersal) # Size of clones The survival and growth rate of a clone after picking is highly dependent on the size of the colony when picked (Figure 28). ![](https://www.moleculardevices.com/sites/default/files/en/assets/training-material/bpd/images/d85b28d7b1934fa7fe653136d222e9f10cdd4673425c080e4d9465c778649a81.jpg) Figure 28: Graph to show area of the colonies picked and resulting growth rate of the clones. If a colony is small, it should not be picked because: $$ That particular clone has a slow growth rate $$ The colony may no longer be viable $$ There is a risk of transferring an insufficient number of cells to the destination plate for the clone to survive. It is recommended to set the “Too Small” group to include colonies $< 0 . 1 ~ \\mathsf { m m } ^ { 2 }$ , though it may be reduced to as low as $0 . 0 3 ~ \\mathsf { m m } ^ { 2 }$ and still achieve good results. # Outgrowth media The destination plate should also be checked immediately after picking in order to verify that picking was successful. If picking efficiency is low (i.e. $< 9 0 %$ of wells have cells in them immediately after picking) the user should check that the correct pins are being used and check that the pin height and camera alignment are set correctly (see ClonePix FL Software Applications Manual). # Reagents and Supplies Please refer to the Molecular Devices website for the latest reagents & supplies, replacement parts and optional extras . [www.moleculardevices.com/genetix](http://www.moleculardevices.com/genetix). # Detection reagents | | | | | --- | --- | --- | | Cat No. | Description | Pack Size | | K8290 | CloneDetect-Rec. Recombinant anti-human detection agent. FITC label. Sterile，azidefree. | 5000U/ 0.5ml | | K8200 | CloneDetect anti-human detection agent. FITC label. Sterile,azide free. | 10000U / 1ml | | K8201 | CloneDetect anti-human detection agent. FITC label. Sterile,azide free.In Atomizerapplicator | 10000U / 5ml | | K8210 | CloneDetect anti-human detection agent.549 label.Sterile,azide free. | 10000U/ 1ml | | K8211 | CloneDetect anti-human detection agent. 549 label. Sterile,azide free. In Atomizer applicator | 10000U / 5ml | | K8212 | CloneDetect anti-human detection agent. 649 label. Sterile,azide free. | 10000U / 1ml | | K8213 | CloneDetect anti-human detection agent. 649 label. Sterile,azide free.In Atomizer applicator | 10000U / 5ml | | K8215 | CloneDetect anti-human CIF (Complex Initiation Factor) | 10000U / 1ml | | K8205 | CloneDetect anti-human detection agent. Gamma chain specific. FITC label. Sterile, azide free. | 10000U/ 1ml | | K8206 | CloneDetect anti-human detection agent. Gamma chain specific. FITC label. Sterile, azide free. In Atomizer applicator | 10000U/ 5ml | | K8252 | CloneDetect anti-human detection agent. Kappa chain specific. FITC label. Sterile, azide-free. | 10000U / 1ml | | K8250 | CloneDetect anti-human detection agent. Kappa chain specific. 549 label. Sterile, azide-free. | 10000U / 1ml | | K8251 | CloneDetect anti-human detection agent. Kappa chain specific. 649 label. Sterile, azide-free. | 10000U / 1ml | | K8255 | CloneDetect anti-human detection agent. IgM specific. FITC label. Sterile,azide- free. | 10000U / 1ml | | K8253 | CloneDetect anti-human detection agent. IgM specific. 549 label. Sterile,azide- free. | 10000U / 1ml | | K8254 | CloneDetect anti-human detection agent.IgM specific. 649 label. Sterile,azide- free. | 10000U / 1ml | | K8220 | CloneDetect anti-mouse detection agent. FITC label. Sterile,azide free. | 10000U / 1ml | | K8221 | CloneDetect anti-mouse detection agent. FITC label. Sterile,azide free.In Atomizerapplicator | 10000U / 5ml | | K8230 | CloneDetect anti-mouse detection agent. 549 label. Sterile,azide free. | 10000U / 1ml | | K8231 | CloneDetect anti-mouse detection agent. 549 label. Sterile,azide free.In Atomizer applicator | 10000U/ 5ml | | K8232 | CloneDetect anti-mouse detection agent. 649 label. Sterile,azide free. | 10000U / | | K8233 | CloneDetect anti-mouse detection agent. 649 label. Sterile,azide free. In Atomizer | 1ml 10000U / | | | | | | --- | --- | --- | | | applicator | 5ml | | K8225 | CloneDetect anti-mouse detection agent. Gamma chain specific. FITC label. Sterile, azide free. | 10000U / 1ml | | K8226 | CloneDetect anti-mouse detection agent. Gamma chain specific.FITC label. Sterile, azide free.In Atomizerapplicator | 10000U / 5ml | | K8235 | CloneDetect anti-mouse CIF (Complex Initiation Factor) | 10000U / 1ml | | K8240 | CloneDetect anti-rat detection agent.FITC label | 10000U/ 1ml | | K8300 | LiveDetect Green (494nm Ex/ 517nm Em). Sterile.In Atomizer applicator | 50ul (for 5ml) | # CloneMatrix | | | | | --- | --- | --- | | Cat No. | Description | Pack Size | | K8510 | CloneMatrix | 1 × 40ml (100ml final media volume) | | K8500 | CloneMatrix | 6 x 40ml (6 x 100ml final media volume) | | K8530 | CloneMatrix: optimized for CHO cells | 1 x 40ml (100ml final media volume) | | K8520 | CloneMatrix:optimized for CHO cells | 6 x 40ml (6 x 100ml final media volume) | # CloneMedia / XPMedia | | | | | --- | --- | --- | | Cat No. | Description | Pack Size | | K8610 | CloneMedia (semi-solid media for hybridomas/myelomas) | 1 × 90ml | | K8600 | CloneMedia (semi-solid media for hybridomas/myelomas) | 6 × 90ml | | K8640 | CloneMedia-G (glutamine free semi-solid media for hybridomas/myelomas) | 1 × 90ml | | K8630 | CloneMedia-G (glutamine free semi-solid media for hybridomas/myelomas) | 6 × 90ml | | K8685 | CloneMedia-HEK (semi-solid media for serum-free HEK 293 cells) | 1 × 90ml | | K8680 | CloneMedia-HEK (semi-solid media for serum-free HEK 293 cells) | 6 x 90ml | | K8710 | CloneMedia-CHO (semi-solid media for CHO-s,CHOK1 and DG44 cells) | 1 × 90ml | | K8700 | CloneMedia-CHO (semi-solid media for CHO-s,CHOK1 and DG44 cells) | 6 × 90ml | | K8740 | CloneMedia-CHO-G (glutamine free semi-solid media for CHO-s,CHOK1 and DG44 cells) | 1 x 90ml | | K8730 | CloneMedia-CHO-G (glutamine free semi-solid media for CHO-s,CHOK1 and DG44 cells) | 6 × 90ml | | K8725 | CloneMedia-CHOK1SV (semi-solid media for CHOK1SV cells; glutamine-free) | 1 × 90ml | | K8720 | CloneMedia-CHOK1SV (semi-solid media for CHOK1SV cells; glutamine-free) | 6 x 90ml | | K8650 | XP Media (liquid media for cell line expansion of hybridoma/myeloma cells) | 500ml | | K8660 | XP Media-G (Glutamine-free liquid media for cell line expansion of hybridoma/myeloma cells) | 500ml | | K8690 | XP Media-HEK (liquid media for cell line expansion of suspension HEK cells) | 500ml | | K8750 | XP Media-CHO (liquid media for cell line expansion of CHO-S cells) | 500ml | | K8760 | XP Media-CHO-G (Glutamine-free liquid media for cell line expansion of CHO-S cells) | 500ml | # PetriWell cell culture plates | | | | | --- | --- | --- | | Cat No. | Description | Pack Size | | W1000 | PetriWell-1 Plate.Black with clear base.Polystyrene.TC treated | 1/40 | | W1005 | PetriWell-1 Plate.Black with clear base.Polystyrene.Non TC treated | 1/40 | | W1007 | PetriWell-1 Plate.Black with clear base.Polystyrene.Collagen coated | 1/40 | | W1009 | PetriWell-1 Plate.Black with clear base.Polystyrene.Poly-D-Lysine coated | 1/40 | | W1050 | PetriWell-1 Plate.Black with clear base.EquiGlass.TC treated | 1/40 | | W1055 | PetriWell-1 Plate.Black with clear base.EquiGlass.Non TC treated | 1/40 | | W1055BC | PetriWell-1 Plate.Black with clear base.EquiGlass.Non TC treated.Bar-coded. | 1/40 | | W1057 | PetriWell-1 Plate.Blackwith clear base.EquiGlass.Collagen coated | 1/40 | | W1059 | PetriWell-1 Plate.Black with clear base.EquiGlass.Poly-D-Lysine coated | 1/40 | | W1100 | PetriWell-6 Plate.Black with clear base.Polystyrene.TC treated | 1/40 | | W1105 | PetriWell-6 Plate.Black with clear base.Polystyrene. Non TC treated | 1/40 | | W1107 | PetriWell-6 Plate.Black with clear base.Polystyrene.Collagen coated | 1/40 | | W1109 | PetriWell-6 Plate.Black with clear base.Polystyrene.Poly-D-Lysine coated | 1/40 | | W1150 | PetriWell-6 Plate.Black with clear base.EquiGlass.TC treated | 1/40 | | W1155 | PetriWell-6 Plate.Black with clear base.EquiGlass. Non TC treated | 1/40 | | W1155BC | PetriWell-6 Plate.Black with clear base.EquiGlass. Non TC treated.Bar-coded. | 1/40 | | W1157 | PetriWell-6 Plate.Black with clear base.EquiGlass.Collagen coated | 1/40 | | W1159 | PetriWell-6 Plate.Black with clear base.EquiGlass.Poly-D-Lysine coated | 1/40 | | W1500 | PetriWell-96 Plate.Black with clear base.Polystyrene.TC treated | 1/40 | | W1505 | PetriWell-96 Plate.Black with clear base.Polystyrene. Non TC treated | 1/40 | | W1507 | PetriWell-96 Plate.Black with clear base.Polystyrene.Collagen coated | 1/40 | | W1509 | PetriWell-96 Plate.Black with clear base.Polystyrene.Poly-D-Lysine coated | 1/40 | | W1510 | PetriWell-96 Plate.Clear. Polystyrene.TC treated | 1/40 | | W1515 | PetriWell-96 Plate.Clear.Polystyrene.Non TC treated | 1/40 | | W1515BC | PetriWell-96 Plate.Clear. Polystyrene. Non TC treated.Bar-coded. | 1/40 | | W1517 | PetriWell-96 Plate.Clear.Polystyrene.Collagen coated | 1/40 | | W1519 | PetriWell-96 Plate.Clear.Polystyrene.Poly-D-Lysine coated | 1/40 | | W1550 | PetriWell-96 Plate.Black with clear base.EquiGlass.TC treated | 1/40 | | W1555 | PetriWell-96 Plate.Black with clear base.EquiGlass. Non TC treated | 1/40 | | W1557 | PetriWell-96 Plate.Black with clear base.EquiGlass.Collagen coated | 1/40 | | W1559 | PetriWell-96 Plate.Black with clear base.EquiGlass. Poly-D-Lysine coated | 1/40 | Other bar-coded plates are available. Please enquire. # Other | | | | | --- | --- | --- | | Cat No. | Description | Pack Size | | K2505 | aQu Clean pin cleaning solution | 1L | | K8080 | Sterilizing Agent (1L per Sachet) | 10 | | K8085 | Sterilizing Agent (1L per Sachet) | 50 | | K8150 | CaliBeads:Fluorescent beads for ClonePix FL.200um diameter. Pan- wavelength. | 100ml | | K8010 | Adherent Cell Picking Reagent-Type A | 1 × 500ml | | K8020 | Adherent Cell Picking Reagent - Type B | 1 × 500ml | | K8030 | Adherent Cell Picking Reagent - Type C | 1 × 500ml | | K8040 | Adherent Cell Picking Reagent - Type D | 1 × 500ml | | K8005 | Adherent Cell Picking Reagent Test Kit Types A- D | 4 × 250ml | | K8100 | ProbeClean: Fluorescently labeled Antibody Clean-up Columns | 50 | # Glossary of Terms # Animal component free media A media free from any animal derived components such as serum or BSA. Plant derived components may be used. # Antibiotic selection An antibiotic resistance gene is often included on the plasmid as a selectable marker. Neomycin and puromycin are the most common examples of this. Selection using this method is rapid, usually being effective in just 48 hours. Transient expressers may still be present at this stage and antibiotic resistance does not select for the location of integration or the copy number present. # Biotherapeutic molecule or biologic A biological product produced from a cell culture whether mammalian or bacterial. The product will generally have some therapeutic use e.g. monoclonal antibody or clotting factor. The product is purified from the cells, generally having been produced on a large scale (50L plus). Culture conditions may be strict to prevent contact with animal based products or contamination with viruses. # Cell line An established population of cells which has come from one progenitor cell. # Chemically defined media A media containing synthesized components rather than those that have come from a biological background. These media tend to be nutrient poor since they do not contain the complexity of factors found in animal derived products. # CHO cell An immortal cell line derived from Chinese Hamster ovary tissue. The cell line is frequently used for production of biotherapeutic molecules. The cell line in its native form is referred to as CHOK1. Modifications to the cell line have led to the development of CHO-s, CHOK1SV, CHO DG44 and CHO DXB11. # Clone A population of cells arising from a single progenitor. # Colony A cluster of cells in contact with each other that originate from one cell. These occur when cells are seeded at low density in semi-solid media or in tissue culture plates as adherent colonies. # Complex Initiation Factor Used to precipitate a secreted IgG around the colony it was secreted from. Another fluorescent detection molecule needs to be applied to detect the secretion complex, e.g. an antigen. # Conditioned media Media which has been incubated with viable cells growing in log phase for 48 hours. The cells release growth factors into the media which conditions it. The cells are removed by filtering the media through a $0 . 2 \\mu \\mathrm { m }$ filter. They do not need to be spun down. This should be made fresh if possible and is usually used to improve the growth of cells grown at a low density. Between $1 0 - 2 0 %$ conditioned media should be sufficient to improve growth. # Confluence The percentage coverage of a well plate or tissue culture flask with cells. # Detection agent Usually an antibody used to signal the presence of a compound of interest (primary detection) e.g. CloneDetect used to signal the presence of secreted antibody from a colony. The detection agent may bind to an unlabelled capture probe designed to precipitate the secreted product (secondary detection). # DHFR Dihydrofolate reductase. Involved in nucleotide synthesis. DG44 cells are knocked out for this gene. # DHFR selection CHO cells deficient in dihydrofolate reductase (DHFR) require hypoxanthine, thymidine and glycine to allow nucleotide production via the salvage pathway. DHFR is re-introduced on a plasmid. When hypoxanthine and thymidine are withdrawn from the media, only transfected cells can survive. Methotrexate can be used as an inhibitor of DHFR activity so that only cells with a high level of DHFR expression can survive. # Dialyzed FBS Commercially available version of Fetal Bovine Serum which has been dialyzed to remove certain products, among them glucose, thymidine and hypoxanthine, each of which plays a part in two of the main selection systems for transfected CHO cells. # ELISA Enzyme Linked Immunosorbant Assay. A method of detecting the concentration of an antigen, usually a secreted antibody or other protein. The most common use of this technique is to coat a microtiter plate with a capture antibody, then add the sample. A detection antibody attached to a horseradish peroxidase enzyme is added. When the substrate for the enzyme is added, the resulting colorimetric change can be used to determine the amount of the specific protein in the sample. # Expansion of clones Culture of cells from a low volume to a high volume. Generally cells should be between 40- $70 %$ confluent before being transferred up to the next volume of media. This is a gradual process designed to keep cells within the log phase throughout the process. # FACS Fluorescence Activated Cell Sorting. Cells are labeled with a fluorescent marker and passed as single cells through a laser which excites the fluorophore. The signal is recorded and the cells are sorted into groups. # FBS Fetal Bovine Serum. Commercially available. Used to supplement cells used for research use but is usually omitted for any product which may be used in therapeutic applications due to the difficulties of ensuring purification from viruses. # Fed batch Similar to an overgrow culture but the culture is fed with fresh nutrients while toxic elements are removed. This is more similar to conditions in a bioreactor. # FITC Fluorescein isothiocyanate. This is the most commonly used fluorophore in bio-therapeutic production. It is usually attached through an amine group. # Gene of interest The recombinant gene introduced into a cell, e.g. a monoclonal antibody or other secreted biologic which the cell is being used to produce for harvest. # GS selection Endogenous glutamine synthase (GS) activity is blocked by the drug methionine sulfoximine (MSX) and cells can only survive if they have additional glutamine synthase introduced on a plasmid. Selection pressure in the form of MSX is maintained throughout culture. # HAT Hypoxanthine, Aminopterin and Thymidine used in combination for the selection of fused hybridoma cells from unfused myeloma fusion partners. # Hybridoma A cell resulting from the fusion of a myeloma and B cell creating an immortal cell line expressing a monoclonal antibody. # Hybridoma fusion The fusion of B cells derived from the spleen of a host animal (usually a mouse) that has been repeatedly immunized against a target molecule. The B cells express the animals’ natural antibody repertoire with antibodies against the target molecule present in high numbers. The cells are fused via electrofusion or PEG to myeloma cells, commonly SP2/0, to immortalize them. # Limiting dilution A cloning technique which works on the principle of diluting cells down to a statistical value of 0.25 cells per well. This should result in 1 in 4 wells containing 1 cell. Wells must be checked for the presence of a single cell only. Wells containing multiple cells cannot be considered clonal. The cells are then grown in the plates until there are sufficient numbers for testing. # Liquid handling The transfer of cells between well plates and flasks. Usually done manually in culture and subculture of cells but can be automated. # Metabolic selection An essential metabolite is withdrawn or an inhibitor applied so that a cell can only survive if it has taken up and integrated a selectable gene on the plasmid. # Methylcellulose A plant based thickening agent used to convert cell culture media to a more viscous form which can allow the formation of colonies and secretion complex in three dimensions. # Mixed population An established population of cells which originates from more than one progenitor cell or has diversified substantially over time creating sub-populations. One sub-population may come to dominate the population due to a higher growth rate. # Monoclonal antibody An antibody that has been derived from a clonal population expressing the same antibody. # MSX Methionine sulfoximine. A drug used to inhibit glutamine synthase to knock down endogenous glutamine production. # MTX Methotrexate. A folate analogue which inhibits the activity of dihydrofolate reductase, preventing nucleotide synthesis. It is used in cancer treatment and to apply selection pressure to transfected DG44 cells. # Multiplexing (on ClonePix FL) Using multiple fluorophores to visualize two different factors e.g. antigen recognition and antibody isotype. Colorized images can be overlaid on the white light background to show colonies positive for one or the other fluorophore or both. # Passage Also called splitting or subculture. This involves maintaining the cells in their log phase of growth by taking a small volume of the growing culture and adding it to fresh media to create a new, lower density culture. # Pick list (ClonePix FL) The list of colonies which fit the criteria specified to the software in terms of size, shape, proximity, fluorescence and order of priority. # Polyclonal antibody An antibody that has been derived from a mixed population of cells expressing antibodies to the same target but with different epitopes and affinities. These can be derived from the sera of an immunized animal or from multiple monoclonal cell lines pooled together. # Population complexity The number of independent chromosomal integration sites represented within the population. This will affect the diversity of the population and the potential for isolating highly expressing cells. This is a function of the transfection efficiency, any targeting of specific locations by the plasmid, time after transfection and any selection conditions used. # Post-pick media The media used to pick the colonies in to. The composition of this media is a crucial factor in re-adaption to liquid media and good growth/productivity. # Productivity The amount of protein produced per cell. A cell line has high productivity if each cell is producing a high level of antibody. This is unrelated to growth rate. # Outgrowth The continued growth of cells from a low number. This is generally used to describe the growth of a confluent well from a colony transferred from semi-solid media back to liquid media (in a 96 well plate). # Overgrow flask Seeding a starting culture at a normal passage density and allowing the culture to reach peak cell density then drop in viability. This is a useful tool for characterization of a cell line by monitoring cell numbers and titer over time. # Seeding cells Diluting cells to a defined number and culturing them in media whether semi-solid or liquid. For colony formation, the seeding density must be low in order to prevent the formation of an undefined lawn of cells. # Selectable marker Usually a gene introduced on the plasmid which confers resistance or metabolic advantage over non-transfected cells, e.g. antibiotic resistance, glutamine synthase or dihydrofolate reductase. An external stimulus is applied to the cells such as a drug to kill non-transfectants or withdrawal of an essential metabolite. # Selection of cells The process of isolating cells or colonies selectively based on one or several parameters e.g. growth, fluorescence, responsiveness to a drug or lack of an essential metabolite. # Semi-solid media A complete media containing all components needed for cell growth but made sufficiently viscous with methylcellulose to allow colony formation in three dimensions, e.g. CloneMedia. # Serum containing medium A medium containing animal derived serum e.g. fetal bovine serum. This is a nutrient rich medium but may be difficult to purify sufficiently to be used in therapeutic use. If the product is for research purposes only then serum containing media provides excellent growth conditions for the cells. # Serum free media A media which lacks animal derived serum e.g. fetal bovine serum. The media is less nutrient rich but also has a lower risk of virus contamination of the product. Other animal components such as BSA may be present. # Shake flask $3 0 \\mathsf { m l }$ Erlenmeyer suspension cultures incubated at $3 7 ^ { \\circ } \\mathsf C \_ { \\iota }$ , $5 %$ ${ \\mathsf { C O } } \_ { 2 }$ on a shaking platform. # Stable cell line An established population of cells which has come from one progenitor cell and shows repeatable growth and productivity over successive generations. # Sub-clone A cell line or colony arising from one daughter cell of a parent cell line. The new cell line has been twice cloned. # Titer The amount of target protein produced by a population of cells. This is a result of the productivity and growth rate of the population. For example a slow growing, high productivity cell line and a fast growing, low productivity cell line may produce comparable titers. # Transfectant A cell line which has taken up the plasmid DNA either stably or transiently. # Transfection The process of introducing DNA into cells via electroporation or chemical agent. # Transfection efficiency The number of cells or percentage of cells which have stably integrated the DNA introduced. This should be determined by the growth of the cells under selective conditions. # Viability The percentage viable cells over dead cells typically measured by Trypan Blue staining and counting on a hemocytometer. # Contact Details # Molecular Devices Queensway, New Milton Hampshire BH25 5NN, UK Tel: $+ 4 4$ (0) 1425 624 600 Fax: $+ 4 4$ (0) 1425 624 700 Web: [www.moleculardevices.com/genetix](http://www.moleculardevices.com/genetix) For all technical queries please contact your nearest Customer Support group. Visit [www.moleculardevices.com/genetix](http://www.moleculardevices.com/genetix) for latest contact details. # Trademarks ClonePix™, CloneSelect™, CellReporter™, HalfBD®, 'NRich®, SlidePath™, Data Arena™ and Image Arena™ are trademarks of Molecular Devices (New Milton) Ltd. Copyright $\\circledcirc$ 2011 by Molecular Devices (New Milton) Ltd All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form by any means, electronic, mechanical, by photocopying, recording, or otherwise, without the prior written permission of Molecular Devices (New Milton) Ltd. Information furnished by Molecular Devices (New Milton) Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Molecular Devices (New Milton) Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use. No license is granted by implication or otherwise under any patent rights of Molecular Devices (New Milton) Ltd. Revised August 2011 ## Live Cell Imaging ![Live Cell Imaging](https://www.moleculardevices.com/applications/media_184a5727c89d66dc52c0f3d8b626e7c2c63b5555c.png?width=1200&format=png&optimize=medium) # Live Cell Imaging Develop assays that can best mimic the in vivo nature of cells **[Download eBook](https://www.moleculardevices.com/en/assets/ebook/dd/img/cellular-imaging-insights "Download eBook")** - [Overview](https://www.moleculardevices.com/applications/live-cell-imaging#overview) - [Workflow](https://www.moleculardevices.com/applications/live-cell-imaging#workflow) - [Resources](https://www.moleculardevices.com/applications/live-cell-imaging#resources) - [Related Products & Services](https://www.moleculardevices.com/applications/live-cell-imaging#related-products-services) ## What is live cell imaging? Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. The ability to study cellular and subcellular structure, function, and organization in living systems aids in the development of assays that are more biologically relevant and that can better predict the human response to new drug candidates. Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells. ![video thumbnail](https://play.vidyard.com/Z9cCK1AnrUBwKxR7drNNXy.jpg) ![](https://play.vidyard.com/Z9cCK1AnrUBwKxR7drNNXy.jpg) Time-lapse video of fibroblast cell division. Time-lapse imaging was performed for a total time of 18 hours. Fibroblasts stably expressed mCherry-H2B (red, nuclei) and GFP-β-tubulin (green, tubulin) ## Benefits of live cell research Live cell imaging is a fundamental research tool in cell biology labs and in a wide variety of industries, and it has led to the discovery of drug targets and candidates as well as the molecular mechanisms involved in diseases. Critical for these researchers, is the ability to develop assays that can best mimic the _in vivo_ nature of cells. Benefits of imaging live cells include: - Monitoring live cells over time provides more than a snapshot of what is occurring in the cells while allowing for the visualization of transient events that may be missed in end-point assays and revealing optimal time-points for end-point assays. - Tight regulation of environmental conditions ensures that the cellular structures and processes being studied are in their native physiological state - Imaging live cells in their natural state minimizes artifacts that can arise from other imaging techniques, such as cell fixation and immunostaining. - The localization and transport of cellular biomolecules along with the progression of multiple pathways can be investigated simultaneously in real-time. - Acquiring images laterally, axially, and temporally enables the generation of 4D images and data - Live cell imaging of molecular dynamics generates qualitative and quantitative data that can’t be gained from other biochemical methods. ## Cellular imaging and analysis workflow Protocols for culturing, plating, and maintaining live cells will vary between different cell models however, the steps below outlines a generalized workflow for [live cell assays.](https://www.moleculardevices.com/applications/cell-imaging "live cell assays.") [![Cellular imaging and analysis workflow](https://www.moleculardevices.com/applications/media_1da8912c8be938eb44a92620ba5308374ad1ef7a5.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/applications/cell-imaging "Cellular imaging and analysis workflow") **[See detailed workflow](https://www.moleculardevices.com/applications/live-cell-imaging#workflow "See detailed workflow")** 1. **Plate cells** into labware of choice (chamber slides, dishes, microplates) 2. **Treat cells** – Perform cell treatments with desired compounds, RNAi, etc. if part of the assay workflow. 3. **Stain for markers** – If required, label cells with the desired fluorophores (fluorescent dyes, fluorescent protein – peptide fusions, etc. 4. **Environmental controls** – If the acquisition will take a long time or if the assay is a live cell time-lapse experiment (continuous or discontinuous), then enable full environmental control including gas, temperature, and humidity. 5. **Acquire cell image** – Place the plate into the [automated imaging system](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-pico "automated imaging system") and start acquiring your live cell images 6. **Analyze cell image** – Use cellular imaging analysis software to run quantitative analysis of live cell images ## Live cell imaging system Sophisticated [live cell imaging systems](https://www.moleculardevices.com/products/cellular-imaging-systems#High-Content-Imaging "live cell imaging systems") provide the ability to regulate environmental conditions to maintain the health and viability of cells while monitoring molecular and cellular dynamics from the single cell to organismal level. From the study of fast-kinetic events to running long-term, time-lapse assays, automated live cell imaging provides the direct observation of dynamic biological processes and the ability to generate a wealth of cellular information - Label-free, brightfield imaging to multi-color fluorescence imaging capabilities provide the flexibility necessary for the multitude of live cell imaging applications - High-acquisition speeds allow for the examination of fast-kinetic events, such as calcium oscillation in cardiomyocytes. - Robust focusing capabilities of automated live cell imaging instruments ensure the maintenance of optimum focus through the entirety of the acquisition, which is crucial for generating accurate live cell imaging data. ## Applications and assays Advancements in automated microscopes with integrated environmental control chambers as well as in fluorescent protein and synthetic fluorophore technology have expanded the capabilities of researchers investigating biological processes in living cells. Our systems for high-content imaging and analysis provide flexible scalability making it easy to evolve your system alongside your research. They feature options and modules to address your specific research needs including objectives, filters, imaging modes, and environmental control. Here we have a collection of applications and assays that utilize these key features for your live cell imaging research: - [3D Live Cell Assays](https://www.moleculardevices.com/applications/live-cell-imaging#3d-live-cell-assays) - [Cell Migration Assays](https://www.moleculardevices.com/applications/live-cell-imaging#cell-migration-assays) - [Cytotoxicity Assays](https://www.moleculardevices.com/applications/live-cell-imaging#cytotoxicity-assays) - [Label-Free Live Cell Assays](https://www.moleculardevices.com/applications/live-cell-imaging#label-free-live-cell-assays) - [Oncology - Cancer Research](https://www.moleculardevices.com/applications/live-cell-imaging#oncology---cancer-research) - [Organoids](https://www.moleculardevices.com/applications/live-cell-imaging#organoids) - [Perform Functional Assays with Living Cells](https://www.moleculardevices.com/applications/live-cell-imaging#perform-functional-assays-with-living-cells) - [Stem Cell Research](https://www.moleculardevices.com/applications/live-cell-imaging#stem-cell-research) - [Subcellular Studies](https://www.moleculardevices.com/applications/live-cell-imaging#subcellular-studies) - [Time-Lapse Imaging](https://www.moleculardevices.com/applications/live-cell-imaging#time-lapse-imaging) ### 3D Live Cell Assays ![3D Live Cell Assays](https://www.moleculardevices.com/applications/media_102fdfc2b411108b3f001876a686434b43538f9b6.png?width=750&format=png&optimize=medium) The integration of three-dimensional (3D) assay models is becoming more widespread to drive translational biology. This has led to significant progress in the development of higher complexity and biologically relevant [3D cell models](https://www.moleculardevices.com/applications/3d-cell-models) to better mimic _in vivo_ environments and responses to drug treatments. Recently, simple, automated workflows, suitable for high-content imaging have become popular. Acquiring measurements from these 3D structures involve acquiring images from different depths (z-planes) and analyzing them in 3D or collapsing the images into a single 2D stack before analysis. - [Acquire data 10X faster from 3D spheroids cultured using Symphony® and VersaGel®](https://www.moleculardevices.com/en/assets/app-note/dd/img/acquire-data-10x-faster-from-3d-spheroids-using-symphony-and-versagel) - [High-Throughput Confocal Imaging of Spheroids for Screening Cancer Therapeutics](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-throughput-confocal-imaging-of-spheroids-for-screening-cancer-therapeutics) - [3D analysis and morphometric characterization of compound effects on cancer spheroid cultures](https://www.moleculardevices.com/en/assets/app-note/dd/img/3d-analysis-and-morphometric-characterization-of-compound-effects-on-cancer-spheroid-cultures) - [High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-content-3d-toxicity-assay-using-ipsc-derived-hepatocyte-spheroids) ### Cell Migration Assays ![Cell Migration Assays](https://www.moleculardevices.com/applications/media_19996188f1ad852cd11ee845a893b0e4c52196f9b.png?width=750&format=png&optimize=medium) The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell time lapse imaging. A "wound" in a confluent monolayer of cells growing in a microplate is created, either by manually creating a scratch or by utilizing special microplates that provide a uniform and reproducible cell-free zone. Monitor cell proliferation, wound healing, migration, and spreading using transmitted light or live cell-compatible fluorescence. These medium to high throughput assays may be used to compare the migration between cells treated with either inhibitory or stimulatory compounds. - [Measure cell migration with discontinuous time-lapse live cell imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/measure-cell-migration-using-discontinuous-time-lapse-live-cell-imaging) - [Evaluate cell migration with FluoroBlok inserts on the SpectraMax MiniMax cytometer](https://www.moleculardevices.com/en/assets/app-note/br/evaluate-cell-migration-with-fluoroblok-inserts-on-the-spectramax-minimax-cytometer) - [Measure cell migration using a simple scratch assay with timelapse live-cell imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/measure-cell-migration-using-simple-scratch-assay-with-live-cell-imaging) ### Cytotoxicity Assays ![Cytotoxicity Assays](https://www.moleculardevices.com/applications/media_16c5773e72c24635bb8fc5a1d7b6aa1c54dcc0fad.png?width=750&format=png&optimize=medium) Development of predictive in vitro assays suitable for safety and efficacy testing is of high interest for improving the drug development process and reducing drug attrition. There has been great interest in using stem cells as tools for screening compounds during early drug development. In this scientific poster, we present results obtained from one of our multi-mode plate readers for performing toxicity assessment of various compounds using stem cell-derived cell models in a high throughput manner: - [High Throughput Cell Based Assays for Toxicity Assessment using SpectraMax® Paradigm Plate Reader](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/br/high-throughput-cell-based-toxicity-assessment-using-spectramax-paradigm-plate-reader.pdf) ### Label-Free Live Cell Assays ![Label-Free Live Cell Assays](https://www.moleculardevices.com/applications/media_101753bf20d663942ee9cfd5f3933bf6db012dca5.png?width=750&format=png&optimize=medium) Label-free imaging and analysis is a requirement for a multitude of live cell biological applications that monitor cell number, confluency, cytotoxicity, development, growth, or proliferation. High contrast brightfield and phase contrast imaging along with robust analysis capabilities provide precise segmentation for the quantification of cells and the assessment of a variety of cellular responses and morphologies. - [Counting cells without cell staining](https://www.moleculardevices.com/applications/cell-counting/counting-cells-without-cell-staining) - [Count cells with or without fluorescent labels using automated imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/count-cells-with-or-without-fluorescent-labels-using-automated-imaging) - [Alternatives to DAPI staining: imaging and counting live cells](https://www.moleculardevices.com/en/assets/app-note/br/alternatives-to-dapi-staining-imaging-and-counting-live-cells) ### Oncology - Cancer Research ![Oncology - Cancer Research](https://www.moleculardevices.com/applications/media_133eafed6bd11826e11f637ad8986815fbafccd1e.jpeg?width=750&format=jpeg&optimize=medium) Cancer researchers need tools that enable them to more easily study the complex and often poorly understood interactions between cancerous cells and their environment, and to identify points of therapeutic intervention. Learn about instrumentation and software that facilitate cancer research using, in many cases, biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the _in vivo_ environment of a tumor or organ. [View Cancer Research](https://www.moleculardevices.com/applications/cancer-research) ### Organoids ![Organoids (3D) multi-cellular microtissues](https://www.moleculardevices.com/applications/media_10fe12d247f46e108eb561412fa3bcbdd6ade65f9.jpg?width=750&format=jpg&optimize=medium) [Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids) are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures. - [Brain Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/brain-organoids) - [Breast Cancer Tumoroids](https://www.moleculardevices.com/applications/3d-cell-models/organoids#breast-cancer-organoids) - [Cardiac Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids#cardiac-organoids) - [Colorectal Cancer (CRC) Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/colorectal-cancer-crc-organoids) - [Intestinal Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/intestinal-organoids) - [Patient-derived organoids (Tumoroids)](https://www.moleculardevices.com/applications/3d-cell-models/organoids/patient-derived-organoids) - [Pulmonary (Lung) Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/pulmonary-lung-organoids) ### Perform Functional Assays with Living Cells ![Perform Functional Assays with Living Cells](https://www.moleculardevices.com/applications/media_12a74f37ced1608a1adf02413c590ef753fb6db4d.png?width=750&format=png&optimize=medium) It is critical for drug screening programs to gain a global view of the cytotoxic effects of potential drug candidates. In combination with traditional cytotoxicity assays, functional assays provide insight into the effects of drugs and compounds on normal cellular function. Sensitive functional assays like calcium flux assays, which utilize a fluorescent dye to monitor changes in intracellular calcium, aid in understanding the full scope of cytotoxicity. Calcium flux assays have been utilized to investigate the effects of compounds on cardiomyocyte pacing and neuron electrophysiology. Maintaining physiological environmental conditions and being able to perform fast kinetic imaging is ideal for running functional assays. - [High-throughput screening of single-cell contractile force using FLECS Technology](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-throughput-screening-of-single-cell-contractile-force-using-flecs-technology) - [Explore cardiac function by measuring calcium oscillation or contraction patterns](https://www.moleculardevices.com/en/assets/app-note/dd/img/explore-cardiac-function-by-measuring-calcium-oscillation-or-contraction-patterns) - [Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids](https://www.moleculardevices.com/en/assets/app-note/dd/img/assessment-of-drug-effects-on-cardiomyocyte-physiology-using-human-ipsc-derived-cardiac-spheroids) ### Stem Cell Research ![Pluripotent stem cells](https://www.moleculardevices.com/applications/media_1a2ea9f9d0bbca920f2ce9acc926e39519683ed42.png?width=750&format=png&optimize=medium) Pluripotent [stem cells](https://www.moleculardevices.com/applications/stem-cell-research) can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The [ImageXpress system](https://www.moleculardevices.com/products/cellular-imaging-systems) has utility in all parts of the stem cell researcher’s workflow, from tracking differentiation, to quality control, to measuring functionality of specific cell types. [Learn more about stem cell research](https://www.moleculardevices.com/applications/stem-cell-research) ### Subcellular Studies ![Subcellular Studies](https://www.moleculardevices.com/applications/media_128016042b9961153ddab201fa991de26d743b67b.png?width=750&format=png&optimize=medium) Live cell imaging permits investigation into a variety of cellular processes involving subcellular compartments and molecules. This includes the monitoring of kinetic processes in real time, such as autophagy, cell signaling, protein trafficking, receptor internalization, endocytosis, and translocation to name a few. Advancements in automated imaging systems as well as in fluorescent protein and synthetic fluorophore technology has expanded the capabilities of researchers to study the dynamic function and structure of organelles and proteins. - [Live cell kinetics assay utilizing the ImageXpress Micro System](https://www.moleculardevices.com/en/assets/app-note/dd/img/live-cell-kinetics-assay-utilizing-the-imagexpress-micro-system) - [Visualize subcellular vesicles to quantitate autophagy in neuronal cells](https://www.moleculardevices.com/en/assets/app-note/dd/img/visualize-subcellular-vesicles-to-quantitate-autophagy-in-neuronal-cells) - [Measuring mitochondrial shape and distribution using the ImageXpress Micro High-Content Imaging System](https://www.moleculardevices.com/en/assets/app-note/dd/img/measuring-mitochondrial-shape-and-distribution-using-the-imagexpress-micro) - [Detection of autophagy using automated imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/detection-of-autophagy-using-automated-imaging) ### Time-Lapse Imaging ![Time-Lapse Imaging](https://www.moleculardevices.com/applications/media_1f6992e56634f668ca9f1130c4a6a2ee84aac7c6c.png?width=750&format=png&optimize=medium) Many live cell imaging applications require maintenance of native cellular environmental conditions and the ability to perform time-lapse imaging. This includes the ability to regulate temperature, pH, gas (O2, CO2), and humidity. Being able to maintain these critical components allows for the preservation of cell health during long-term, multi-day assays. The ImageXpress suite of imaging systems provide the seamless integration of environmental control, robust imaging, and powerful analysis enabling the acquisition and visualization of kinetic images and data. - [Monitor multiple stages of apoptosis with live cell kinetic imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/monitor-multiple-stages-of-apoptosis-with-live-cell-kinetic-imaging) - [Monitor cell proliferation and cell cycle in real time](https://www.moleculardevices.com/en/assets/app-note/dd/img/monitor-cell-proliferation-and-cell-cycle-in-real-time) - [Evaluating cell cycle inhibitors using a live cell assay](https://www.moleculardevices.com/en/assets/app-note/dd/img/evaluating-cell-cycle-inhibitors-using-live-cell-assay) - [Intelligent time-lapse imaging to enhance live cell assay development](https://www.moleculardevices.com/en/assets/app-note/dd/img/intelligent-time-lapse-imaging-to-enhance-live-cell-assay-development) ## Latest Resources [![3D Biology: The paradigm shift in next-generation drug discovery [Interactive infographic]](https://www.moleculardevices.com/applications/media_1c761770b532a641e8ebb023c3aebc9edda9ae595.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Blog Jan 07, 2025 ### [3D Biology: The Paradigm Shift in Next-Gen Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Discover how 3D biology is revolutionizing drug discovery by providing… [Learn more](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) [![T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/2024/t-cell-induced-morphological-analysis-of-colorectal-cancer-organoids-using-ai.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) Scientific Poster Nov 20, 2024 ### [T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) To overcome the challenges of using PDOs in large-scale applications, we… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) [![High-speed Phenotypic Profiling using Imaging with AI Image Analysis](https://www.moleculardevices.com/applications/media_17e80e29479c2f02b0253dc50615e29328c86d21c.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) Application Note Jul 30, 2025 ### [High-speed phenotypic profiling using a next generation high-content imaging platform with AI-enabled image analysis](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) Learn how our next-gen imager and software with AI Imaging platform enable… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) [![Transforming High-Content Screening with Intelligent Imaging & AI Analysis](https://www.moleculardevices.com/applications/media_15049d0d1dbe419d6c35797cd87e83d58ab471b4e.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) Videos and Webinars Jul 17, 2025 ### [Transforming high-content screening with intelligent imaging and AI analysis](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) Discover how our AI-powered analysis platform enhances high-content… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) [![From bottlenecks to breakthroughs](https://www.moleculardevices.com/sites/default/files/2025/automating-cell-culture-scalable-research.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/dd/3d-biology/automating-cell-culture-scalable-research) eBook Apr 28, 2025 ### [From bottlenecks to breakthroughs](https://www.moleculardevices.com/en/assets/ebook/dd/3d-biology/automating-cell-culture-scalable-research) Discover how automating cell culture boosts research reliability, reduces… [Learn more](https://www.moleculardevices.com/en/assets/ebook/dd/3d-biology/automating-cell-culture-scalable-research) [![Machine learning-assisted automation of complex 2D and 3D cell culture and assay models](https://www.moleculardevices.com/sites/default/files/2025/machine-learning-assisted-automation-of-2d-3d-cell-culture.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/machine-learning-assisted-automation-of-2d-3d-cell-culture.pdf) Scientific Poster Mar 31, 2025 ### [Machine learning-assisted automation of complex 2D and 3D cell culture and assay models](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/machine-learning-assisted-automation-of-2d-3d-cell-culture.pdf) Discover automate cell culture for standard and complex protocols,… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/machine-learning-assisted-automation-of-2d-3d-cell-culture.pdf) [![Novel Workflow to Quantify Interaction between T-Cell and Patient-Derived Organoids](https://www.moleculardevices.com/applications/media_158bb9eb5178f1a23f2a4c0dfee3a8ed7066fc1b5.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/novel-workflow-interaction-between-t-cells-and-patient-derived-organoids) Application Note Mar 18, 2025 ### [A novel workflow to quantify the interaction between T-cells and patient-derived organoids](https://www.moleculardevices.com/en/assets/app-note/dd/img/novel-workflow-interaction-between-t-cells-and-patient-derived-organoids) Explore a novel workflow to quantify T-cell interaction with… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/novel-workflow-interaction-between-t-cells-and-patient-derived-organoids) [![Evaluating compound toxicity effects on healthy intestinal organoids using high-content imaging](https://www.moleculardevices.com/applications/media_18f661d8613f97e969478d678f74d9e9c949d03f2.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/evaluation-compound-toxicity-effects-on-healthy-intestinal-organoids-using-imaging) Application Note Feb 04, 2025 ### [Evaluating compound toxicity effects on healthy intestinal organoids using high-content imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/evaluation-compound-toxicity-effects-on-healthy-intestinal-organoids-using-imaging) We present a method to evaluate toxicity using 3D mouse intestinal… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/evaluation-compound-toxicity-effects-on-healthy-intestinal-organoids-using-imaging) [![Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/applications/media_1e10a63a7266b3a8a7f6725d51bf0cbf8dc9dcae9.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) News Jan 15, 2025 ### [Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) Molecular Devices Introduces the ImageXpress HCS.ai High-Content Screening… [Learn more](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) [![3D Biology: The paradigm shift in next-generation drug discovery [Interactive infographic]](https://www.moleculardevices.com/applications/media_1c761770b532a641e8ebb023c3aebc9edda9ae595.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Blog Jan 07, 2025 ### [3D Biology: The Paradigm Shift in Next-Gen Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Discover how 3D biology is revolutionizing drug discovery by providing… [Learn more](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) [![T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/2024/t-cell-induced-morphological-analysis-of-colorectal-cancer-organoids-using-ai.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) Scientific Poster Nov 20, 2024 ### [T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) To overcome the challenges of using PDOs in large-scale applications, we… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) [![High-speed Phenotypic Profiling using Imaging with AI Image Analysis](https://www.moleculardevices.com/applications/media_17e80e29479c2f02b0253dc50615e29328c86d21c.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) Application Note Jul 30, 2025 ### [High-speed phenotypic profiling using a next generation high-content imaging platform with AI-enabled image analysis](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) Learn how our next-gen imager and software with AI Imaging platform enable… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/high-speed-phenotypic-profiling-using-imaging-platform-with-ai-analysis) [![Transforming High-Content Screening with Intelligent Imaging & AI Analysis](https://www.moleculardevices.com/applications/media_15049d0d1dbe419d6c35797cd87e83d58ab471b4e.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) Videos and Webinars Jul 17, 2025 ### [Transforming high-content screening with intelligent imaging and AI analysis](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) Discover how our AI-powered analysis platform enhances high-content… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/dd/img/transforming-high-content-screening-with-imaging-ai-analysis) **[View all Resources](https://www.moleculardevices.com/applications/live-cell-imaging#resources "View all Resources")** ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=live-cell-imaging "Speak to a specialist")** ## Live Cell Imaging and Analysis Workflow The described live cell imaging workflow is a generalized example of a continuous long-term time-lapse live cell assay. This workflow breaks down the steps from cell plating to image analysis and highlights the materials and instrumentation necessary to run a live cell assay including an automated high-content imaging system and cellular imaging and analysis software. [Live Cell Imaging & Analysis Workflow](https://www.moleculardevices.com/fragments/applications/live-cell-imaging-workflow.html "Live Cell Imaging & Analysis Workflow") [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Resources for Live Cell Imaging [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products & Services [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Cell Plating Tutorial ![Blue Image](https://www.moleculardevices.com/en/assets/tutorials-videos/reagents/media_1b94b5d556ad08f0d9695d60f6bf50c340929476b.jpeg?width=1200&format=jpeg&optimize=medium) Videos & Webinars # Preparing and Plating Cells in Semi-Solid Medium Plating cells in the viscous semi-solid medium may be challenging to first-time users. Watch our 4-minute tutorial to learn some tips and tricks on how to plate cells in this medium more efficiently. Our suite of semi-solid media includes: - [CloneMedia CHO Growth A](https://www.moleculardevices.com/products/culture-media-reagents/clonemedia-cho-growth-a "CloneMedia CHO Growth A") - [CloneMedia for mouse hybridoma generation](https://www.moleculardevices.com/products/culture-media-reagents "CloneMedia for mouse hybridoma generation") - [Other CloneMedia](https://www.moleculardevices.com/products/culture-media-reagents "Other CloneMedia") ![video thumbnail](https://play.vidyard.com/myX6vBrg7s99h8TXHXUAfs.jpg) ![](https://play.vidyard.com/myX6vBrg7s99h8TXHXUAfs.jpg) ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Website Terms of Use ![Blue Image](https://www.moleculardevices.com/media_1b94b5d556ad08f0d9695d60f6bf50c340929476b.jpeg?width=1200&format=jpeg&optimize=medium) # Online Terms of Use #### Molecular Devices Online Terms of Use These Terms of Use apply to this website (the “Site”) that is operated by Molecular Devices, LLC. and its affiliates (“Molecular Devices”). Throughout the Site, the terms “we,” “us” and “our” refer to Molecular Devices. Molecular Devices offers the Site, including all information, tools and services available from the Site, to you, the user, conditioned upon your acceptance of all the terms, conditions, policies and notices stated here. Your use of the Site constitutes your agreement to these Terms of Use. #### Accuracy, Completeness and Timeliness of Information on the Site Molecular Devices is not responsible if information made available on the Site is not accurate, complete or current. The material on the Site is provided for general information only and should not be relied upon or used as the sole basis for making decisions without consulting primary, more accurate, more complete or more timely sources of information. Any reliance on the material on the Site is at your own risk. The Site may contain certain historical information. 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THIS DISCLAIMER APPLIES, WITHOUT LIMITATION, TO ANY DAMAGES OR INJURY ARISING FROM ANY FAILURE OF PERFORMANCE, ERROR, OMISSION, INTERRUPTION, DELETION, DEFECTS, DELAY IN OPERATION OR TRANSMISSION, COMPUTER VIRUSES, FILE CORRUPTION, COMMUNICATION-LINE FAILURE, NETWORK OR SYSTEM OUTAGE, YOUR LOSS OF PROFITS, OR THEFT, DESTRUCTION, UNAUTHORIZED ACCESS TO, ALTERATION OF, LOSS OR USE OF ANY RECORD OR DATA, AND ANY OTHER TANGIBLE OR INTANGIBLE LOSS. YOU SPECIFICALLY ACKNOWLEDGE AND AGREE THAT NEITHER MOLECULAR DEVICES NOR ITS SUPPLIERS SHALL BE LIABLE FOR ANY DEFAMATORY, OFFENSIVE OR ILLEGAL CONDUCT OF ANY USER OF THE SITE. YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY OF THE ABOVE CLAIMS OR ANY DISPUTE WITH MOLECULAR DEVICES IS TO DISCONTINUE YOUR USE OF THE SITE. YOU AND MOLECULAR DEVICES AGREE THAT ANY CAUSE OF ACTION ARISING OUT OF OR RELATED TO THE SITE MUST COMMENCE WITHIN ONE (1) YEAR AFTER THE CAUSE OF ACTION ACCRUES OR THE CAUSE OF ACTION IS PERMANENTLY BARRED. 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Molecular Devices reserves the right, at its own expense, to assume exclusive defense and control of any matter otherwise subject to indemnification by you and, in such case, you agree to cooperate with Molecular Devices in the defense of such matter. #### Jurisdiction and Applicable Law The laws of the State of California govern these Terms of Use and your use of the Site, and you irrevocably consent to the jurisdiction of the courts located in the State of California for any action arising out of or relating to these Terms of Use. We recognize that it is possible for you to obtain access to the Site from any jurisdiction in the world, but we have no practical ability to prevent such access. The Site has been designed to comply with the laws of the State of California and of the United States. If any material on the Site, or your use of the Site, is contrary to the laws of the place where you are when you access it, the Site is not intended for you, and we ask you not to use the Site. You are responsible for informing yourself of the laws of your jurisdiction and complying with them. #### Changes to These Terms of Use Molecular Devices reserves the right, at its sole discretion, to change, modify, add or remove any portion of these Terms of Use, in whole or in part, at any time, by posting revised terms on the Site. It is your responsibility to check periodically for any changes we make to the Terms of Use. Your continued use of the Site after any changes to the Terms of Use or other policies means you accept the changes. In the event we make material changes to the Terms of Use, notice of the changes will be posted on the homepage of the Site and the revised terms will take effect thirty days after their publication on the Site. **Entire Agreement and Admissibility** This agreement and any policies or operating rules posted on the Site constitute the entire agreement and understanding between you and Molecular Devices with respect to the subject matter thereof and supersede all prior or contemporaneous communications and proposals, whether oral or written, between the parties with respect to such subject matter. A printed version of these Terms of Use shall be admissible in judicial or administrative proceedings based on or relating to use of the Site to the same extent and subject to the same conditions as other business documents and records originally generated and maintained in printed form. #### Severability If any provision of this agreement is unlawful, void or unenforceable, the remaining provisions of the agreement will remain in place. #### How to Contact Us If you have any questions or comments about these Terms of Use or the Site, please contact us by e-mail at [info@moldev.com](mailto:info@moldev.com "info@moldev.com") or write to us at: **3860 N First Street** **San Jose, CA 95134** **USA** Thank you for visiting our Site. ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## SaaS Agreement Overview ![Blue Image](https://www.moleculardevices.com/media_1b94b5d556ad08f0d9695d60f6bf50c340929476b.jpeg?width=1200&format=jpeg&optimize=medium) # Software as a Service (SaaS) Agreement ### Software as a Service (SaaS) Agreement This Software as a Service Agreement (“Agreement”) is by and between the Molecular Devices entity identified on the applicable Order Form (“MolDev”) and the Customer (as defined below). #### DEFINITIONS AND INTERPRETATION 01. **Definitions.** 02. **Affiliate:** Any entity that controls, is controlled by, or is under common control with the subject entity. "Control," for purposes of this definition, shall mean the power to direct or cause the direction of the management and policies of an entity, whether through the ownership of voting stock, by contract or otherwise. 03. **Customer:** the entity purchasing the Services from MolDev as identified in the applicable Order Form. 04. **Customer Data:** All electronic data and information submitted, entered, uploaded or retrieved in any form by or for Customer to the Services. For the avoidance of doubt, Customer Data does not include Usage Data. 05. **Customer Support:** standard technical support, for query and problem resolution, in relation to the Services. 06. **Documentation:** technical literature, specifications and user guide for the Services, as updated from time to time, accessible or supplied with the Services, in printed and/or electronic format, provided that updates to the Documentation will not result in a material reduction in the level of performance or availability of the Services for the duration of the applicable Subscription Term. 07. **Fees:** The fees and charges as set forth in the applicable Order Form and this Agreement that are payable by Customer to MolDev under this Agreement. 08. **Intellectual Property:** Patents, utility models, rights to inventions, copyright and related rights, trademarks and service marks, trade names and domain names, rights in designs, rights in computer software, database rights, trade secrets, and any other intellectual property rights, including all applications for (and rights to apply for and be granted), renewals or extensions of, and rights to claim priority from, such rights and all similar or equivalent rights or forms of protection which subsist or will subsist, now or in the future, in any part of the world. 09. **Order Form:** A MolDev order form, proposal, or other ordering document in the name of and executed by Customer and accepted by MolDev that specifies the Services to be provided by MolDev under this Agreement, or a purchase order issued by Customer and accepted by MolDev for the Services identified in MolDev’s corresponding quotation, which Order Form is incorporated herein by reference. 10. **Party:** In the singular, either Customer or MolDev as the context may dictate, or in the plural (“ **Parties**”), both Customer and MolDev. 11. **Personal Data:** Customer Data relating to an identified or identifiable natural person. 12. **Services:** the software-as-a-service (SaaS) services that are ordered by Customer as set forth in an Order Form and made available online by MolDev to Customer, including Updates, associated modules, plug-ins, and offline components, as the context requires. 13. **Subscription Term** has the meaning set forth in Sec. 12.2 (Term of User Subscriptions) of this Agreement. 14. **Updates:** Versions of the Services or components thereof, released by MolDev to its customers from time to time under MolDev’s support/update/development plans, which may remedy or provide a workaround to instances of non-conformance of the Services to the Documentation or provide general improvement or enhancement to the Services. 15. **Usage Data:** Information generated by the Services pertaining to the Customer’s use of the Services, used to monitor and continually improve the Services, which does not include within it any element of Customer Data. 16. **User:** A named person who is authorized by Customer to use the Services. Each User accessing the Services will be identified by a unique username and password. 17. **User Subscription** has the meaning set forth in Sec. 2.1 (User Subscriptions) of this Agreement. 18. This Agreement is comprised of: 19. (a) the terms and conditions of this Software as a Service Agreement, 20. (b) the Order Form(s), and 21. (c) the documents expressly incorporated into the above or by written agreement between the parties, attached as exhibits, schedules or appendices to this Agreement, or as expressly referenced within this Agreement. 22. In the case of conflict or ambiguity between any provision contained in the body of this Software as a Service Agreement and any provision contained in the Order Form, exhibits, except for contractual clauses relating to the transfer of Personal Data in Exhibit C, schedules or appendices, the provision in the body of this Master Subscription Services Agreement shall take precedence unless such other document identifies the provision of the Software as a Service Agreement to be modified, specifically describes the modification and is executed by authorized representatives of both Parties. The foregoing notwithstanding, any pre-printed terms in any invoices or Customer’s purchase orders that are different from or in addition to those in this Agreement are hereby expressly rejected and void and shall not apply to any order for or provisioning of Services. #### PROVISION OF SERVICES 1. **User Subscriptions.** Unless otherwise specified in the applicable Order Form, Customer purchases Services as a subscription to use the Services (“ **User Subscription**”) for a defined **Subscription Term**. Fees for User Subscriptions are due annually in advance before the Subscription Term begins. Additional User Subscriptions for the Services may be added during a Subscription Term either at the then current MolDev list prices or as otherwise agreed to by the Parties in an Order Form. User Subscriptions added during a Subscription Term will be billable upon receipt of an Order Form for such additional User Subscriptions and will terminate on the same date as the termination date of the current Subscription Term. 2. **Provision of Services.** Pursuant to the terms and conditions of this Agreement, MolDev will make the Services available to Customer and its Users by granting access to a cloud-based version of the software hosted on behalf of MolDev for the duration of the Subscription Term. The software shall reside on server(s) operated on behalf of MolDev. Users shall have access to the software solely via the Internet, and Customer shall not receive any software code. MolDev may apply Updates from time to time in its sole discretion, and in accordance with this Agreement, as part of its on-going intent to improve the Services and Customer’s use of the Services. 3. **Personal Data.** To the extent that Customer Data comprises Personal Data, the terms of the Data Protection Addendum attached hereto as **Exhibit C**, which is incorporated into this Agreement, shall apply. 4. **Subcontractors.** Customer acknowledges that MolDev uses the services of third-party subcontractors, including but not limited to, third-party datacenter, for the provisioning of the Services and Customer Support under this Agreement. MolDev shall be responsible for any act or omission by its third-party subcontractors. #### INFORMATION AND SYSTEM SECURITY 1. MolDev’s obligations for information and system security measures in relation to the Services are set forth in MolDev’s Product Security Features Description attached to Customer’s first Order Form which is incorporated into this Agreement by reference. A copy of the Product Security Features Description is also available to Customer upon request. 2. MOLDEV DISCLAIMS ANY LIABILITY FOR UNAUTHORIZED ACCESS, USE OR RELEASE OF ANY CUSTOMER DATA, UNLESS SUCH ACCESS, USE OR RELEASE RESULTS FROM MOLDEV’S FAILURE TO MEET ITS SECURITY OBLIGATIONS SET FORTH IN THIS SECTION 3. 3. MolDev will back up Customer Data as provided in the Product Security Features Description. Without prejudice to the above, Customer is also responsible for retaining backup copies of all Customer Data, and for implementing its own disaster-recovery plan. Customer will use reasonable security precautions, in accordance with its industry standards, in light of its use of the Services. #### CUSTOMER RESPONSIBILITIES 1. **Usage Limits.** 1. Services may not be accessed by more Users than the number of User Subscriptions specified for the Services in the applicable Order Form. Each User accessing the Services will be identified by a unique username and password. User Subscriptions are strictly for the individual designated Users only and may not be shared. User Subscriptions may, however, be reassigned to a new individual to replace an existing User who no longer requires use of, or access to, the Services. Customer will: (a) use commercially reasonable efforts to prevent unauthorized access to, or use of, the Services; (b) notify MolDev promptly if it becomes aware of any such unauthorized access or use; and (c) use the Services solely for its business as contemplated by this Agreement. 2. Unless otherwise agreed in an executed Order Form , (a) MolDev will allocate the specified amount of usage time, data transfer and data storage as set forth in the applicable Order Form to Customer’s use of the Services and (b) MolDev reserves the right, with prior written notice to Customer, to charge additional fees for Customer’s use that is in excess of such allocated usage limits at its then-current list prices, provided that MolDev also notifies the Customer in writing if the Customer approaches the allocated usage limits prior to the end of the applicable Subscription Term. 2. **Customer Responsibilities Regarding Customer Data.** Customer shall have sole responsibility for the accuracy, quality, legality, reliability and appropriateness of all Customer Data, and the means by which Customer Data was acquired. Except for the pseudonymized patient identification number, Customer shall not provide any “special categories of personal data” or health care provider data to MolDev and shall not use Services to process such data. For the purposes of this Section, “special categories of personal data” shall have the meaning as set forth in the EU General Data Protection Regulation, such data is also known as “sensitive data” under other laws and regulations regarding the processing of Personal Data. 3. **Terms of Use.** Customer agrees to and shall comply with the Terms of Use set forth in **Exhibit A** attached to and incorporated into this Agreement. 4. **Suspension.** If any User: (a) violates this Agreement; (b) is using the Service in a manner that MolDev reasonably believes may cause a security risk, a disruption to others’ use of the Services, or liability for MolDev, MolDev may, upon written notice to Customer specifying the non-compliance in reasonable detail: (i) suspend the account of such User; and (ii) require the Customer to remove the non-compliant Customer Data from the Services immediately . If the Customer fails to do so, MolDev may, at its own option, delete the non-compliant Customer Data from the Services or terminate access to such Customer Data. #### CUSTOMER SUPPORT 1. Standard Customer Support for query and issue resolution in relation to the Services will be provided to Customer in accordance with Customer Support Description attached hereto as **Exhibit B** and incorporated into this Agreement. #### FEES AND PAYMENT 1. **Fees.** Customer shall pay all Fees specified in the applicable Order Form. Customer acknowledges that: (a) Fees are based on Services purchased and not actual usage; (b) payment obligations are non-cancellable, and any Fees paid are non-refundable, unless otherwise specified in this Agreement; and (c) the number of User Subscriptions purchased in the applicable Order Form cannot be decreased during the Subscription Term unless otherwise agreed in an Order Form executed by both Parties. 2. **Invoicing and Payment.** MolDev shall issue invoices in accordance with this Agreement and the applicable Order Form. Customer is responsible for providing MolDev with complete and accurate invoicing and contact information and promptly notifying MolDev of any changes to such information. All non-disputed invoices issued by MolDev to Customer are due net thirty (30) days from the invoice date. All levies, duties or similar governmental assessments of any nature, including, for example, value-added, sales, use or withholding taxes (collectively, “Taxes”) due on Fees will be applied to a Customer invoice as required by law. 3. **Overdue Amounts.** If any undisputed invoiced amount is not received by MolDev by the due date, then without limiting its rights or remedies, MolDev may: (a) invoice Customer for the late payment interest at the rate of 1.5% of the outstanding balance per month, or the maximum rate permitted by law, whichever is lower; and (b) upon written notice, suspend Customer’s access to the Services until payment is received in full. #### PROPRIETARY RIGHTS 1. **MolDev Rights in the Services.** Subject to the limited rights expressly granted hereunder, MolDev and its licensors reserve all rights, title and interest in and to the Services and Documentation, including all related Intellectual Property rights. No rights are granted to Customer hereunder other than as expressly set forth herein. 2. **MolDev License.** Subject to the terms and conditions of this Agreement, MolDev hereby grants to Customer and its Users a non-exclusive, fee-bearing, non-sublicensable, non-transferable (except as set forth in Section 14.5, Assignment) license to use the Services and Documentation for Customer’s internal business operations during the specified Subscription Term. Subject to the limited license granted in this Section 7.2, Customer acquires no right, title or interest from MolDev under this Agreement in or to the Services. 3. **Limited License to Use Customer Data.** Customer hereby grants to MolDev a limited, non-exclusive and non-transferable (except as provided in Section 14.5, Assignment) license to access, use, copy, reproduce, process, transmit and display Customer Data submitted to the Services by Customer Users, or provided by Customer to MolDev, for the limited purpose of: (a) providing the Services and Customer Support and displaying the Customer Data to Users for the duration of this Agreement; and (b) generating Usage Data. 4. **Right to Ideas.** MolDev shall have a royalty-free, worldwide, transferable, sub-licensable, irrevocable, perpetual license to use or incorporate into the Services, Documentation and Customer Support, any suggestions, ideas, enhancement requests, recommendations or other feedback (collectively, the “ **Feedback**”) provided by Customer or its Users relating to the operation and use of the Services. #### CONFIDENTIALITY 1. **Confidential Information.** For the purposes of this Agreement, “ **Confidential Information**” means all information disclosed by a Party or its Affiliates (“ **Disclosing Party**”) to the other Party (“ **Receiving Party**”), whether orally or in writing, that is designated as confidential or that reasonably should be understood to be confidential given the nature of the information and the circumstances of disclosure. Confidential Information does not include any information that: (a) is or becomes generally known to the public without breach of any obligation owed to the Disclosing Party; (b) was known to the Receiving Party prior to its disclosure by the Disclosing Party without breach of any obligation owed to the Disclosing Party; (c) is received from a third party without restriction and without breach of any obligation owed to the Disclosing Party; or (d) was independently developed by the Receiving Party without use of the Disclosing Party’s Confidential Information. 2. **Protection of Confidentiality.** Each Party agrees to protect the confidentiality of the Confidential Information of the other Party in the same manner that it protects the confidentiality of its own proprietary and confidential information, using at least reasonable care. The Receiving Party shall not disclose or use any Confidential Information of the Disclosing Party for any purpose outside the scope of this Agreement, except with the Disclosing Party’s prior written permission. Notwithstanding the foregoing, each Party shall have the right to disclose the other Party’s Confidential Information to such of its Affiliates, directors, officers, employees, contractors, accountants, financial advisors and legal counsel having a need to know the Confidential Information (each a “ **Representative**”) for the purpose of this Agreement, provided that, prior to any such disclosure to a Representative, the Representative has agreed in writing to be bound by obligations of confidentiality and non-use on terms at least as restrictive as the terms contained herein. In addition, each Party shall be responsible for any breach of this Agreement by its Representatives. 3. **Legally Required Disclosure.** The Receiving Party may disclose Confidential Information of the Disclosing Party to the extent compelled by law, regulation, regulatory authority, or order of a governmental agency, or court of competent jurisdiction to do so, and as required for the purposes of regulatory filings, prosecuting or defending litigation, or complying with applicable law (including regulations promulgated by securities exchanges), provided the Receiving Party gives the Disclosing Party prior written notice of such disclosure (to the extent legally permitted). 4. **Injunctive Relief.** Each party agrees that any actual or threatened disclosure or misappropriation of Confidential Information would constitute immediate, irreparable harm to the other party for which monetary damages may be an inadequate remedy. Therefore, in addition to any other rights and remedies that may be available to the parties at law or in equity, each party may seek injunctive relief for any such actual or threatened disclosure without the posting of a bond. 5. **Survival.** The obligations set forth in this Section 8 shall survive until five (5) years from the termination or expiration of this Agreement. #### REPRESENTATIONS/WARRANTIES AND DISCLAIMERS 1. **Mutual Warranties.** Each Party represents and warrants that: (a) it has validly entered into this Agreement and has the legal power to do so, and (b) it will comply with all applicable international, national, state, regional and local laws and regulations applicable to such Party’s activities hereunder, including the laws, rules, and regulations of the United States which restrict the export and re-export of commodities and technical data of United States origin. 2. **MolDev Warranties.** MolDev warrants that: (a) it will provide the Services, and Customer Support in a professional manner consistent with industry standards; and (b) the Services shall perform in accordance with the Documentation during the applicable Subscription Term. 3. **Customer Warranties.** Customer represents and warrants on behalf of itself and its Users that it: (a) has all necessary rights to use the Customer Data and to permit MolDev to use Customer Data in connection with the Services without violating any fiduciary duty, duty of confidentiality, contractual obligation or applicable laws; (b) will not use, or permit the use of, the Services for illegal, fraudulent, or unethical purposes or otherwise in a manner that is likely to give rise to civil or criminal liability of MolDev; and (c) will not interfere with or impede the ability of others to use the Services. Customer shall verify that it and its Users comply with the provisions of the Agreement applicable to use of the Services and shall be liable for any and all use of and access to the Services by any Users. 4. **Mutual Disclaimer.** EXCEPT AS EXPRESSLY SET FORTH IN THIS AGREEMENT, NEITHER PARTY MAKES ANY OTHER WARRANTY, REPRESENTATION OR OTHER COMMITMENT OF ANY KIND, WHETHER EXPRESS OR IMPLIED, STATUTORY OR OTHERWISE, AND EACH PARTY SPECIFICALLY DISCLAIMS ALL IMPLIED WARRANTIES, INCLUDING, WITHOUT LIMITATION, ANY WARRANTIES, REPRESENTATIONS OR OTHER COMMITMENTS OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR OF SATISFACTORY QUALITY, TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW. #### INDEMNIFICATION 1. **Indemnification by MolDev.** If a third party makes a claim against Customer, its officers, directors or employees alleging that Customer’s use of the Services for its intended normal purposes as contemplated hereunder infringes the Intellectual Property rights of a third party, MolDev shall: (a) pay all reasonable costs to defend Customer; and (b) pay any damages assessed against Customer in a final judgement by a court of competent jurisdiction or any settlement that MolDev has agreed upon with such third party, subject to Section 10.4, provided; however, that the indemnification obligations set forth in this Section 10.1 shall not apply (i) with respect to any claim of infringement based upon any modifications to the Services made by Customer or a third party not authorized by MolDev, or (ii) to the extent the claims arise from Customer’s negligence or wilful misconduct. 2. **Indemnification by Customer.** To the extent permitted by law, if a third party makes a claim against MolDev, its officers, directors or employees alleging that the Customer Data or Customer’s use of the Services, except as contemplated hereunder, infringes the Intellectual Property rights of a third party, Customer shall: (a) pay all reasonable costs to defend MolDev; and (b) pay any damages assessed against MolDev in a final judgment by a court of competent jurisdiction or any settlement that Customer has agreed upon with such third party, subject to Section 10.4; provided, however, that the indemnification obligations set forth in this Section 10.2 shall not apply to the extent the claims arise from MolDev’s’s negligence or wilful misconduct. 3. **Remedy.** MolDev may, at its option and expense, attempt to resolve any Claim described in Section 10.1 by: (a) modifying the Services to avoid the alleged infringement, without materially altering the Services; (b) obtaining a license to permit Customer’s use of the Services as contemplated by this Agreement; or (c) terminate the license grant set forth in this Agreement with respect to the Services and entitle Customer to receive a refund for any amounts paid in advance that are attributable to the unused portion of the Subscription Term. 4. SECTIONS 10.1 and 10.3 STATE CUSTOMER’S SOLE REMEDY AND MOLDEV’SS SOLE OBLIGATION WITH RESPECT TO INFRINGEMENT CLAIMS. 5. **Notice and Control.** The indemnification obligations set forth in Sec. 10.1 and 10.2 are contingent upon the indemnified party: (a) promptly giving written notice of the claim to the indemnifying party, (b) giving the indemnifying party sole control over the defence and settlement of the claim and (c) providing to the indemnifying party, on its request and at the indemnifying party’s cost, all reasonable assistance. The indemnifying party shall not enter into any settlement of any claim under Section 10 that imposes any obligation on the indemnified party or admits that the indemnified party has any liability for such claim without the indemnified party’s prior written consent. #### LIMITATION OF LIABILITY 1. **Limitation of Liability.** EXCEPT FOR EITHER PARTY’S INDEMNIFICATION OBLIGATIONS, OR CLAIMS ARISING FROM EITHER PARTY’S GROSS NEGLIGENCE, WILFUL MISCONDUCT OR FRAUD, NEITHER PARTY’S LIABILITY ARISING OUT OF OR RELATED TO THIS AGREEMENT WILL EXCEED THE AMOUNT PAID BY THE CUSTOMER IN FEES IN THE 12 MONTHS PRECEDING THE INCIDENT THAT GAVE RISE TO SUCH CLAIM. 2. **Exclusions.** EXCEPT FOR CLAIMS ARISING FROM EITHER PARTY’S GROSS NEGLIGENCE, WILFUL MISCONDUCT, OR FRAUD, IN NO EVENT WILL EITHER PARTY HAVE ANY LIABILITY TO THE OTHER PARTY FOR ANY LOSS OF PROFITS, REVENUES OR INDIRECT, SPECIAL, INCIDENTAL, CONSEQUENTIAL, COVER OR PUNITIVE DAMAGES, WHETHER AN ACTION IS IN CONTRACT OR TORT AND REGARDLESS OF THE THEORY OF LIABILITY, EVEN IF A PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. THE FOREGOING DISCLAIMER WILL NOT APPLY TO THE EXTENT PROHIBITED BY LAW. #### TERM AND TERMINATION 1. **Term of Agreement.** This Agreement commences on the date the Customer’s first Order Form is accepted by MolDev and continues until all User Subscriptions granted in accordance with this Agreement have expired or have been terminated. Except as otherwise provided herein, neither Party may terminate this Agreement or any User Subscriptions without cause. 2. **Term of User Subscriptions.** User Subscriptions start on the date specified in the applicable Order Form and continue for the subscription term specified therein (“ **Subscription Term**”). If no commencement date is specified in the Order Form the User Subscription commencement date shall be five (5) business days from the date of acceptance of the applicable Order Form by MolDev, or, if Customer purchases Services with a MolDev instrument, five (5) business days after: (a) the date of installation of such instrument, if installed by MolDev; or (b) delivery of the instrument to Customer. Unless otherwise specified in the applicable Order Form, at the end of the initial Subscription Term, User Subscriptions shall automatically renew for additional twelve (12) month periods at MolDev’s then current list price, unless either Party gives written notice of non-renewal at least sixty (60) days prior to the end of the then current Subscription Term. If the per unit User Subscription pricing for renewal is not specified on the applicable Order Form, then the per unit User Subscription pricing will be MolDev’s then current list price unless otherwise agreed to by the Parties in writing. 3. **Termination for Cause.** A Party may terminate this Agreement for cause: (a) upon thirty (30) days’ written notice to the other Party of a material breach if such breach remains uncured at the expiration of such period; or (b) if the other Party becomes the subject of a petition in bankruptcy or any other proceeding relating to insolvency, receivership, liquidation or assignment for the benefit of creditors. 4. **Refund or Payment upon Termination.** If Customer terminates this Agreement for cause in accordance with Section 12.3 above, MolDev will refund Customer any prepaid Fees covering the remainder of the applicable Subscription Term after the date of termination. If MolDev terminates this Agreement for cause in accordance with Section 12.3, Customer will pay all Fees otherwise payable to MolDev through the end of the Subscription Term. 5. **Return of Customer Data upon Termination.** Upon the effective date of any expiration or termination of this Agreement by either Party: (a) all rights and licenses granted to either Party will terminate; and (b) except as otherwise stated herein, each Party will immediately, in accordance with the other Party’s instructions, either destroy and certify destruction or return to the other Party all Confidential Information of the other Party (and in the case of MolDev also Customer Data), in its possession, custody or control in whichever form held, unless such Confidential Information or Customer Data is required to be held by a Party for regulatory, audit or legal reasons. Upon Customer’s written request, within thirty (30) days of expiration or termination of the Agreement, MolDev will make available to Customer for download a file of Customer Data in a commonly readable format. #### GENERAL 01. **Waiver.** No failure or delay by a Party to exercise any right or remedy provided under this Agreement or by law shall constitute a waiver of that or any other right or remedy. 02. **Entire agreement.** This Agreement, the Order Form(s) and the documents annexed as appendices to this Agreement or otherwise referred to herein, contain the whole agreement between the parties relating to the subject matter hereof and supersede all prior agreements, arrangements and understandings between the parties relating to that subject matter. 03. **Variation.** No variation of this Agreement shall be effective unless it is in writing and signed by the Parties’ authorised representatives. 04. **Severance.** If any court or competent authority finds that any provision of this Agreement (or part of any provision) is invalid, illegal or unenforceable, that provision or part-provision shall, to the extent required, be deemed to be deleted, and the validity and enforceability of the other provisions of this Agreement shall not be affected. 05. **Assignment.** This Agreement is not assignable by either Party, whether by operation of law or otherwise, without the prior written consent of the other Party (which shall not be unreasonably withheld or delayed); provided, however, that either Party may assign this Agreement as part of a transfer of all or substantially all of the assets of the transferring Party to any third-party (including by operation of a merger, acquisition, or other combination), by providing written notice of such assignment to the other Party. Any purported assignment in violation of this provision shall be void and of no effect. 06. **Third-party rights.** The Parties confirm their intent not to confer any rights on any third parties by virtue of this Agreement. 07. **No partnership or agency.** Nothing in this Agreement is intended to, or shall be deemed to, establish any partnership or joint venture between any of the Parties, constitute any Party the agent of another Party, or authorise any Party to make or enter into any commitments for or on behalf of any other Party. 08. **Force majeure.** Each Party shall be excused performance of its obligations under or pursuant to this Agreement if and to the extent that its performance is delayed, hindered or prevented by any circumstances beyond its reasonable control including (but not limited to) acts of God or the public enemy, war, riots, fire, explosion, terrorist acts, natural disasters, floods, government action, telecommunications failures, Internet service provider or hosting facility failure, and denial of service attacks, strikes and seriously adverse weather conditions. Either Party may terminate this Agreement upon written notice to the other Party if the force majeure event continues for more than thirty (60) days. 09. **Notices.** All Notices shall be in writing and sent to the other Party marked for the attention of the person at the address set forth below or as otherwise notified in writing from time to time. Any notice or other document correctly addressed shall be deemed delivered in three business days following the date of dispatch by first-class mail (in the U.S.), on the date of confirmed delivery by a nationally recognized courier service, with all postage or delivery charges prepaid (outside of the U.S.) or on the date of delivery as confirmed by the signature of the recipient for special or signed for delivery or delivery by hand. Notices to Customer shall be delivered to the attention of the Customer’s representative at the address indicated on the applicable Order Form. Notices to MolDev shall be delivered to the attention of MolDev’s VP of Sales at the address indicated on the applicable Order Form with a copy to the Legal Department at Molecular Devices, 3860 N. First Street, San Jose, CA 95134. 10. **Governing law and jurisdiction.** This Agreement and any dispute or claim arising out of or in connection with it or its subject matter or formation shall be governed by the laws of the country and state where MolDev is located, without reference to its conflicts of law principles. 11. **Survival.** The following provisions of this Agreement and any other provisions that by their nature are intended to survive termination of this Agreement, shall survive any termination or expiration of this Agreement: Sections 1, 7, 8, 9, 10, 11, 12 and 13. #### Exhibit A #### Terms of Use **Use of the Services.** In these Terms of Use, “ **you**” means Customer. If you have a valid verification code received from your company or login details received from Core Life Analytics B.V. and/or its affiliates (“Core Life Analytics”) or its authorized reseller Molecular Devices, provided that you comply with these Terms of Use and the Agreement, Molecular Devices grants you a personal, non-exclusive, non-transferable, limited right to enter and use the Services during the applicable Subscription Term. Unless otherwise specified, the Services are for research use only which expressly excludes any use for medical or diagnostic purposes. You will not use the Services for any purpose that is unlawful or prohibited by the Terms of Use. You may not use the Services in any manner that could damage, disable, overburden, or impair any technology used in providing the Services (such as its servers, network(s) or other related technology to the Services). You may not attempt to gain unauthorized access to the Services, other accounts, computer systems or networks connected to any of the Services, through hacking, password mining or any other means. You may not obtain or attempt to obtain any materials or information through any means not intentionally made available to you through the Services. By way of example, and not as a limitation, you agree that when using the Services, you will not: a. Copy, modify, distribute, sell, or lease any part of the Services. b. Reverse engineer or attempt to extract any of our source code unless you have our written permission or applicable law lets you do so. c. Upload, or otherwise make available, files that contain images, photographs, software or other material protected by intellectual property laws, including, by way of example, and not as limitation, copyright or trademark laws (or by rights of privacy or publicity) unless you own or control the rights thereto or have received all necessary consent to do the same. d. Use any material or information, including images or photographs, which are made available through the Services in any manner that infringes any copyright, trademark, patent, trade secret, or other proprietary right of any party. e. Upload files that contain viruses, Trojan horses, worms, time bombs, cancelbots, corrupted files, or any other similar software or programs that may damage the operation of another's computer or property of another. f. Falsify or delete any copyright management information, such as author attributions, legal or other proper notices or proprietary designations or labels of the origin or source of software or other material contained in a file that is uploaded. g. Restrict or inhibit any other user from using the Services. h. Violate any applicable laws or regulations. MolDev may deny you access to the Services at any time if you breach any obligation arising from these Terms of Use. **Member Account, Password, and Security** If any of the Services requires you to open an account, you must complete the registration process by providing your current, complete and accurate information as prompted by the applicable registration form. You will also choose a password and a username. You are entirely responsible for maintaining the confidentiality of your password and account. Furthermore, you are entirely responsible for any and all activities that occur under your account. You agree to notify MolDev immediately of any unauthorized use of your account or any other actual or suspected breach of security that you become aware of. You may not use anyone else’s account at any time. **Materials provided to the Service** MolDev and its contractors involved in providing the Services do not claim ownership of the materials you provide to the Services. By uploading, inputting, providing or submitting your materials you represent and warrant that you own or otherwise control all of the rights to your materials as described in these Terms of Use including, without limitation, all the rights necessary for you to provide, upload, input or submit the materials. **Intellectual property** All rights, title and interest in and to the Services including patents, copyrights, trademarks, trade names, service marks, designs, media, trade secrets and other intellectual property rights, and any goodwill associated therewith, are owned by, or licensed to Core Life Analytics. #### Exhibit B #### Customer Support Description 1. **Problem Resolution.** Customer will promptly report to MolDev any problems arising in the Services or Documentation and will provide assistance or information as may reasonably be required by MolDev in relation to the diagnosis of such problems. When a problem is an instance of non-conformance of the Services to the Documentation it will be rated with a priority level in accordance with the MolDev standard operating procedures for customer support and responded to in accordance with the timescales and with the resources allocated for the assigned priority level. 2. **Services Updates.** MolDev may, from time to time, during the Subscription Term, provide Updates to the Services, and make them available to Customer. Where possible, MolDev shall inform Customer of any Update at least 24 hours prior to when an Update is to be applied to the Services. 3. **Customer Support Contacts and Hours.** MolDev will respond to Customer’s requests related to reported problems via telephone or email between the hours of 8:00 a.m. and 5:00 p.m. (local time of the MolDev location set forth on the applicable Order Form) Monday through Friday, excluding holidays. A list of standard holidays will be provided to Customer upon request. Customer may contact MolDev’s Customer Support team by phone or email designated by MolDev which shall be provided to Customer upon Services purchase or activation. 4. **Preventive Maintenance, Scheduled Downtime and Service Availability.** MolDev will deliver on a regular basis new patches, bug fixes and updates for the Services (Preventive Maintenance). MolDev will inform the Customers about the regular Preventive Maintenance hours if MolDev is of the opinion that it may interrupt the use of the Services. Scheduled downtime will be scheduled to occur on weekends and holidays whenever possible. The total amount of scheduled downtime will not exceed twenty-four (24) hours during any year and otherwise availability of the Services shall be as set forth in the Product Security Features Description. No individual period of scheduled downtime shall exceed four (4) hours. MolDev will use commercially reasonable efforts to provide Customer with immediate e-mail notification to the point of contact set forth in the electronic record of User registration for the Services as soon as it becomes aware of any actual or potential unscheduled downtime of the Services that is expected to last more than two (2) hours, as well as continual periodic updates during the unscheduled downtime regarding MolDev’s progress in remedying the unavailability and the estimated time that the Services will next be available. 5. **Exclusions.** MolDev shall have no obligation to provide support or remedy Services performance issues if the issue arises from: (a) factors outside MolDev’ direct and reasonable control, including any force majeure event; (c) Customer’s failure to maintain the necessary environmental conditions for use of the Services as provided in the Documentation; (d) Customer’s use of the Services in combination with any equipment or product not provided by MolDev or not designated by MolDev in writing for use with the Services, or any fault in any such non-MolDev equipment or product; (e) any breach of the Customer's obligations under this Agreement howsoever arising; or (f) User error. #### Exhibit C #### Data Protection Addendum (DPA) 01. **Personal Data.** If the Customer submits any Customer Data that comprises Personal Data to the Services, Customer agrees that it acts as Data Controller under applicable Data Protection Laws and Regulations and MolDev and/or its Affiliates act as individual Data Processors on behalf of Customer. 02. **Data Protection.** Each party shall comply with Data Protection Laws and Regulations in connection with its obligations under this Data Protection Addendum (DPA). 03. **Description of processing.** The processing of Personal Data shall be for the duration of the Agreement, for the purpose of providing the Services, and include categories of personal data consisting of basic contact information of Customer’s representatives such as first name, last name and email address. 04. **Processing Terms.** When processing personal data on behalf of Customer, MolDev shall: 05. **4.01** only process personal data on Customer’s written instructions, unless required otherwise by applicable Data Protection Laws and Regulations; 06. **4.02** ensure that all MolDev personnel, who have access to personal data, are subject to suitable confidentiality obligations; 07. **4.03** implement and maintain reasonable technical and organizational measures designed to prevent personal data breach, and if it becomes aware of personal data breach MolDev shall notify Customer without undue delay; 08. **4.04** be generally authorized to use subprocessors, provided that MolDev shall remain fully liable for any of its subprocessors, and, in addition, shall use reasonable endeavors to notify Customer of any changes to its subprocessors, and take into account any reasonable objections of Customer in relation to such changes. Upon Customer’s request, MolDev shall provide Customer with the list of subcontractors authorized to access Personal Data in connection with the Agreement. The parties acknowledge that MolDev engages subcontractors qualified to support offerings that are made generally available to MolDev customers, including the Services, and that selection of subcontractors is not a customizable part of MolDev’s offerings. 09. **4.05** taking into account the nature of the processing, at Customer’s cost and expense, assist Customer by appropriate technical and organizational measures, insofar as this is possible, provide such assistance as Customer may reasonably request to meet its obligations under Data Protection Laws and Regulations in responding to requests from data subjects exercising their rights under Data Protection Laws and Regulations; 10. **4.06** taking into account the nature of the processing and information available to MolDev, at Customer’s cost and expense, provide reasonable assistance to Customer in meeting its obligations under Data Protection Laws and Regulations relating to security, personal data breach notification, data protection impact assessments and prior consultation with supervisory authorities; 11. **4.07** at the choice of Customer, delete or return all Personal Data on Customer’s request or the termination of the Agreement unless required otherwise by applicable Data Protection Laws and Regulations; and 12. **4.08** upon request and at Customer’s cost and expense, make available to Customer, for inspection on MolDev’s premises only, the information necessary to demonstrate compliance with the obligations set out in this DPA and allow for and contribute to audits conducted by Customer or another auditor mandated by Customer and approved by MolDev, provided that Customer gives MolDev at least thirty (30) days’ prior written notice of its intention to carry out an audit. This notice shall include a detailed work plan for the audit. Any third party involved in the audit must agree to MolDev’s confidentiality undertakings. All reports, materials or other information produced by MolDev or a subprocessor during the course of the audit or otherwise resulting from the audit, will be the Confidential Information of MolDev and subject to the provisions of the Agreement. #### International Transfers. 1. MolDev shall be entitled to transfer Personal Data from one country to another country in connection with providing the Services provided there is a lawful basis to do so. If requested by either Party, the Parties shall do all things reasonably necessary to give effect to such transfer, including updating the DPA or putting in place another data transfer mechanism appropriate to comply with Data Protection Laws and Regulations. 2. Any transfer of Personal Data subject to the General Data Protection Regulation of the European Union to a jurisdiction that is not within the European Economic Area will be governed by the terms of the Standard Contractual Clauses for processors approved by the European Commission which are incorporated herein by reference. 3. Any transfer of Personal Data subject to the United Kingdom GDPR to another jurisdiction will be governed by the applicable contractual clauses most recently approved by the UK privacy regulator for transfer of Personal Data out of the UK, unless another legal mechanism for the transfer applies. 4. Any other international transfer of Personal Data requiring a data transfer agreement containing specific terms under Data Protection Laws and Regulations will be governed by such terms. #### Definitions. 13. “Data Controller” means a natural or legal person, public authority, agency or any other body who alone or jointly with others determines the purposes and means of the processing of Personal Data. 14. “Data Processor” means A natural or legal person, public authority, agency or any other body who processes Personal Data on behalf of the Data Controller. 15. “Data Protection Laws and Regulations” means all applicable laws and regulations for a particular country/ jurisdiction regarding the processing of Personal Data as may be revised or updated from time to time. 16. “data subject” means any natural person and/ or juristic person (i.e. a body of persons, a corporation, a partnership, or other legal entity that is recognized under applicable Data Protection Law as the subject of rights and duties) whose personal data is being collected, held or processed; 17. “processing” means any operation or set of operations performed on Personal Data, 18. “personal data breach” means a breach of security leading to the accidental or unlawful destruction, loss, alteration, unauthorized disclosure of , or access to, personal data. ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Hybridoma Antibody Production ![Hybridoma Workflow for Antibody Discovery](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1475c17e55c9597ee0a6a9dd9167ea67c763a4bf2.jpeg?width=1200&format=jpeg&optimize=medium) # Hybridoma Workflow for Antibody Discovery - [Overview](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/hybridoma#overview) - [Resources](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/hybridoma#resources) - [Related Products & Services](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/hybridoma#related-products-services) ## Hybridoma generation and screening of large antibody libraries Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique [monoclonal antibodies](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs "monoclonal antibodies") at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease. [In this video](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/hybridoma-workflow "In this video"), Justin Dranschak, manager for BioPharma platforms, presents our solution for a hybridoma workflow and references the systems to aid in your research. ![video thumbnail](https://play.vidyard.com/XT7k1HMuRMAyrUUh5S75A7.jpg) ![](https://play.vidyard.com/XT7k1HMuRMAyrUUh5S75A7.jpg) ## Hybridoma workflow ![Hybridoma workflow : Generation and screening of antibody libraries using hybridomas](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_12a708b6b1997cf4e2e98e59ef26b18d9989ba28b.png?width=750&format=png&optimize=medium) #### Step 1: Fusion The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma. #### Step 2: Clone screening The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies. #### Step 3: Cell growth Cell growth is determined by monitoring cell divisions over a given period of time using label-free imaging. #### Step 4: Specificity and cross reactivity The process of analyzing the ability of an antibody to bind to only one target antigen. #### Step 5: Binding affinity and internalization Binding affinity is the strength of the binding interaction between a single biomolecule (e.g. protein or DNA) to its ligand/binding partner (e.g. drug or inhibitor). Internalization is the process of monitoring the ability of a given particle to enter into the cell. **[Speak to a specialist](https://www.moleculardevices.com/quote-request?cid=984&pid=hybridoma-workflow-for-antibody-discovery "Speak to a specialist")** ## COVID-19 related research solutions ![COVID-19 research](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1a1258349613250125288add6b185c5aab035aed6.jpeg?width=750&format=jpeg&optimize=medium) ### Accelerate your COVID-19 research Molecular Devices is committed to supporting scientists that are researching COVID-19 cellular response and vaccine development by offering technology and solutions that you can deploy rapidly. [Go to COVID-19 Research](https://www.moleculardevices.com/applications/coronavirus-research "Go to COVID-19 Research") ![Workflow solutions for vaccine development](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_10ec83e80906a76971edbd4b6a9b56a3312b9ed7e.jpeg?width=750&format=jpeg&optimize=medium) ### Workflow solutions for vaccine development View a variety of virus-related workflows – from antigent/immunogen and antibody discovery to stable cell line development. [Go to Vaccine Development Workflows](https://www.moleculardevices.com/applications/coronavirus-research/vaccine-development "Go to Vaccine Development Workflows") ![COVID-19 Applications for critical research](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1ec800878ffd48ae811462be78d8c74a7e0de0c54.jpeg?width=750&format=jpeg&optimize=medium) ### Applications for critical research Learn more about how our technology can help your research of COVID-19 and other infectious diseases for vaccine development, therapeutics and diagnostics. [Go to Coronavirus Vaccine Research](https://www.moleculardevices.com/applications/coronavirus-vaccine-research "Go to Coronavirus Vaccine Research") [![ClonePix FL and Intracellular Staining for High Producer Cells Lines for Monoclonal Antibody Manufacturing](https://www.moleculardevices.com/default-meta-image.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Publication Nov 23, 2020 ### [Sequential screening by ClonePix FL and intracellular staining facilitate isolation of high producer cell lines for monoclonal antibody manufacturing](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Discover how ClonePix FL and intracellular staining to isolate high… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) [![Cloning Methods vs. CloneSelect Single-Cell Printer f.sight using CHO Cell Lines used for Monoclonal Antibody](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_143099d09b27f9453c10fc8601c62aec9c58c437f.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) Application Note Jul 19, 2020 ### [Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) Explore the comparison of cloning methods vs. CloneSelect Single-Cell… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) [![The role of Monoclonal Antibodies against COVID-19](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_111c6f6e3dec93fe1bebeb8f6ddac17412d7cba4d.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) Blog Apr 13, 2022 ### [The role of Monoclonal Antibodies against COVID-19](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) Explore the critical role of monoclonal antibodies against COVID-19. See… [Learn more](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) [![COVID-19: Diagnostics, Vaccines, and Therapeutic Antibody Development](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1d50cbc872e3406366c0090cde5b4dd7cfe3c6bf7.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) Blog Feb 22, 2022 ### [COVID-19 Timeline: Diagnostics, Vaccines, and Therapeutic Antibody Development](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) Explore the COVID-19 timeline as we outline workflow solutions for… [Learn more](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) [![Qualitative Measurement of SARS-CoV-2 IgG Antibodies in Serum Samples](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_179cc19856ed8e0c0a5804787453f4ee1a9461331.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/br/qualitative-measurement-of-sars-cov-2-spike-and-nucleocapsid-igg-antibodies-in-serum-samples) Application Note May 30, 2021 ### [Qualitative measurement of SARS-CoV-2 spike and nucleocapsid IgG antibodies in serum samples](https://www.moleculardevices.com/en/assets/app-note/br/qualitative-measurement-of-sars-cov-2-spike-and-nucleocapsid-igg-antibodies-in-serum-samples) Learn how to qualitatively measure SARS-CoV-2 spike and nucleocapsid IgG… [Learn more](https://www.moleculardevices.com/en/assets/app-note/br/qualitative-measurement-of-sars-cov-2-spike-and-nucleocapsid-igg-antibodies-in-serum-samples) [![Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays](https://www.moleculardevices.com/images/citation-card-thumbnail.webp?width=800&format=webp&optimize=medium)](https://www.nature.com/articles/s41596-021-00536-y) Citation Apr 22, 2021 ### [Citations : Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays](https://www.nature.com/articles/s41596-021-00536-y) Virus neutralization assays measure neutralizing antibodies in serum and… [Learn more](https://www.nature.com/articles/s41596-021-00536-y) [![Hybridoma Workflow | Molecular Devices](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1faf93fe75f2cbecf6ba8181eeb70e0669e754c28.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/hybridoma-workflow) Videos and Webinars Dec 01, 2020 ### [Hybridoma Workflow](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/hybridoma-workflow) In this video, Justin Dranschak present hybridoma workflow solution for… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/hybridoma-workflow) [![Importance of Clonality in Biopharmaceutical Cell Lines | Molecular Devices](https://www.moleculardevices.com/default-meta-image.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/industry-view-on-relative-importance-of-clonality-of-biopharmaceutical-producing-cell-lines) Publication Nov 23, 2020 ### [Industry view on the relative importance of clonality of biopharmaceutical-producing cell lines](https://www.moleculardevices.com/newsroom/in-the-news/industry-view-on-relative-importance-of-clonality-of-biopharmaceutical-producing-cell-lines) Understand industry views on the significance of clonality in the… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/industry-view-on-relative-importance-of-clonality-of-biopharmaceutical-producing-cell-lines) [![ClonePix FL and Intracellular Staining for Monoclonal Antibody](https://www.moleculardevices.com/default-meta-image.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Publication Nov 23, 2020 ### [ClonePix FL and intracellular staining facilitate isolation of high producer cell lines for monoclonal antibody manufacturing](https://www.moleculardevices.com/newsroom/in-the-news/clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Learn about critical parameters and calculations for ClonePix FL colony… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) [![ClonePix FL and Intracellular Staining for High Producer Cells Lines for Monoclonal Antibody Manufacturing](https://www.moleculardevices.com/default-meta-image.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Publication Nov 23, 2020 ### [Sequential screening by ClonePix FL and intracellular staining facilitate isolation of high producer cell lines for monoclonal antibody manufacturing](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) Discover how ClonePix FL and intracellular staining to isolate high… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing) [![Cloning Methods vs. CloneSelect Single-Cell Printer f.sight using CHO Cell Lines used for Monoclonal Antibody](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_143099d09b27f9453c10fc8601c62aec9c58c437f.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) Application Note Jul 19, 2020 ### [Comparison of traditional cloning methods vs. CloneSelect Single-Cell Printer f.sight using CHO cell lines commonly used for monoclonal antibody production](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) Explore the comparison of cloning methods vs. CloneSelect Single-Cell… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/comparison-of-traditional-cloning-methods-vs-cloneselect-single-cell-printer-fsight-using-cho-cell-lines) [![The role of Monoclonal Antibodies against COVID-19](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_111c6f6e3dec93fe1bebeb8f6ddac17412d7cba4d.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) Blog Apr 13, 2022 ### [The role of Monoclonal Antibodies against COVID-19](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) Explore the critical role of monoclonal antibodies against COVID-19. See… [Learn more](https://www.moleculardevices.com/lab-notes/clone-screening/the-role-of-monoclonal-antibodies-against-covid-19) [![COVID-19: Diagnostics, Vaccines, and Therapeutic Antibody Development](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/media_1d50cbc872e3406366c0090cde5b4dd7cfe3c6bf7.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) Blog Feb 22, 2022 ### [COVID-19 Timeline: Diagnostics, Vaccines, and Therapeutic Antibody Development](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) Explore the COVID-19 timeline as we outline workflow solutions for… [Learn more](https://www.moleculardevices.com/lab-notes/clone-screening/covid-19-timeline-diagnostics-vaccines-antibody-discovery) **[View all Resources](https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/hybridoma#resources "View all Resources")** ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=hybridoma-workflow-for-antibody-discovery "Speak to a specialist")** ## Resources related to COVID-19 cellular responses and vaccine development [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Systems to accelerate your COVID-19 cellular response and vaccine development We have validated and compliant laboratory solutions including microplate readers, microplate washers, biopharma and cellular imaging systems to meet your research needs. 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Often scientists use the HAT (hypoxanthine-aminopterin-thymidine) method to accomplish this task. During the fusion process, three types of cells are present: (1) unfused myeloma cells that are deficient in an enzyme called HGPRT, (2) unfused spleen cells, and (3) fused hybridoma cells. - Unfused spleen cells are easily selected against since they do not replicate in culture.\ - Unfused myelomas can be selected against using media containing HAT. The aminopterin found in the medium blocks the _de novo_ DNA nucleotide synthesis pathway. Typically when the _de novo_ pathway is blocked, cells will then utilize the salvage pathway as an alternative means to replicate (only if hypoxanthine and thymidine are present). However, these myelomas are unable to do so since they are deficient in an enzyme called HGPRT, which is required for the salvage pathway. Hence, myelomas are unable to replicate in culture. \ - Only hybridomas survive. Hybridomas inherit a functioning HGPRT enzyme from the spleen cells, so even though the _de novo_ pathway is blocked, they can still use the salvage pathway to replicate. Please watch our tutorial video for more details. ![video thumbnail](https://play.vidyard.com/XWD9vBtYzixFLHzuDoWPLj.jpg) ![](https://play.vidyard.com/XWD9vBtYzixFLHzuDoWPLj.jpg) ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## IQ/OQ/PM Services ![IQ/OQ/PM Services](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_15d27e7c1a16f5ace8b4a621284429bfcf9401184.jpeg?width=1200&format=jpeg&optimize=medium) # IQ/OQ/PM Services ![IQ/OQ/PM services](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_1a99cdd8a2a2b4573d89efd08e0118476ce08d7a1.jpeg?width=750&format=jpeg&optimize=medium) Installation qualification, operational qualification, preventive maintenance, and repair coverage for microplate readers and washers - [Overview](https://www.moleculardevices.com/products/gxp-compliance-solutions/iq-oq-pm-services#overview) - [Resources](https://www.moleculardevices.com/products/gxp-compliance-solutions/iq-oq-pm-services#resources) - [Related Products & Services](https://www.moleculardevices.com/products/gxp-compliance-solutions/iq-oq-pm-services#related-products-services) ## Preserve instrument documentation in a digital and compliant format IQ/OQ/PM services preserve instrument documentation in a digital and compliant format. Ensure ongoing compliance of your Molecular Devices instruments and be audit ready with comprehensive validation, maintenance, and repair documentation. ![Simplify instrument documentation storage with a digital format](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_1334a02c89b9ae1e2a0364bcb402b3eae509a3f49.png?width=750&format=png&optimize=medium) ### Simplify instrument documentation storage with a digital format Digital laboratory records for instrument inspection, maintenance, testing, and calibration provide a convenient document storage method aligned with electronic recordkeeping ![Satisfy multiple regulatory guidelines](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_101544db59fdd1fa67e29dbdf518cc762f245cd0e.png?width=750&format=png&optimize=medium) ### Satisfy multiple regulatory guidelines for instrument validation and maintenance Standardized IQ/OQ/PM services performed by certified field service engineers satisfy FDA 21 CFR and EudraLex Volume 4 guidelines for instrument validation and maintenance. Molecular Devices only certifies operational qualification on instruments when performed by a Molecular Devices certified engineer ![video thumbnail](https://play.vidyard.com/HDy2TDw4esBC141RtXTUNT.jpg) ![](https://play.vidyard.com/HDy2TDw4esBC141RtXTUNT.jpg) GMP-IQ/OQ PMOQ for microplate readers and washers ## Features ![Installation Qualification (IQ)](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_12b071e593632eea06f52ea914b73ab1b4ed92763.png?width=750&format=png&optimize=medium) ### Installation Qualification (IQ) Verifies and documents that all necessary components required for operation are received and properly installed in accordance with Molecular Devices installation protocols. ![Operational Qualification (OQ)](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_19ef51f2d0b1c4853e3862de15269638ccca26e9c.png?width=750&format=png&optimize=medium) ### Operational Qualification (OQ) Tests every subsystem of the reader or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. ![Preventive Maintenance (PM)](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_12794b07662cce7ad0f4092ec44166c892a17537d.png?width=750&format=png&optimize=medium) ### Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. Our comprehensive multipoint inspection and testing ensures each microplate reader or washer meets our product operational specifications. ## GxP Compliance Solutions – Assure data integrity and compliance for GMP/GLP laboratories. Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory. - Best-in-class **SpectraMax Microplate Readers and Washers** - Industry-leading [SoftMax Pro GxP Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software "SoftMax Pro GxP Software") – our most secure software to achieve full FDA 21 CFR Part 11 compliance - **IQ/OQ/PM Services** preserve instrument documentation in a digital and compliant format - **Software Installation & Qualification Services** verify and document that required components are installed to operational specification - **SpectraTest Validation Plates and Recertification Services** tests the performance of your microplate reader to ISO 17025 standards **[GxP Compliance Solutions](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP Compliance Solutions")** _![](https://play.vidyard.com/uUqe3yFG3vCLcasHfCPznR.jpg)_ ## Latest Resources [![Tech Blast | Quality assurance in drug discovery](https://www.moleculardevices.com/newsroom/in-the-news/media_1afafa5f83011a22a46b14db9009918587e3a82e1.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) Publication Dec 15, 2021 ### [Tech Blast \| Quality assurance in drug discovery](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) In this Tech Blast episode, Tim Bolus from Molecular Devices explores the… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) [![FDA 21 CFR Part 11: Ensuring Regulatory Compliance in GMP & GLP Labs](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_184e0cde7872849688a9c5ccef93ca80c21c136c5.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) Blog May 09, 2021 ### [FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) Know the importance of FDA 21 CFR Part 11 compliance in GMP and GLP labs.… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) [![FUJIFILM Bacterial Endotoxin Assays on SpectraMax Readers](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_124bed76591c3553e70912a62d119fd06b1228334.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) Application Note Jan 07, 2025 ### [FUJIFILM bacterial endotoxin assays on SpectraMax readers](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) Discover how FUJIFILM bacterial endotoxin assays on SpectraMax readers… [Learn more](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) [![Software Validation Test Suite: Operational Qualification (OQ) & Performance Qualification (PQ)](https://www.moleculardevices.com/images/default-card-thumbnail.webp?width=800&format=webp&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) Brochure Sep 17, 2024 ### [Software Validation Test Suite: Operational Qualification (OQ) & Performance Qualification (PQ)](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) Molecular Devices provides validation services for SoftMax® Pro GxP Data… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) [![Automated Testing Tool For Functional Testing of SoftMax Pro GxP Software Suite](https://www.moleculardevices.com/sites/default/files/2024/media_10ce0ed9affc07c94a77ec6f154fb575bd63b125d.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/automated-testing-tool-for-functional-testing-of-softmax-pro-gxp.pdf) Flyer Mar 15, 2024 ### [Automated Testing Tool For Functional Testing of SoftMax Pro GxP Software Suite](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/automated-testing-tool-for-functional-testing-of-softmax-pro-gxp.pdf) Our newest service innovation for software functional testing features an… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/automated-testing-tool-for-functional-testing-of-softmax-pro-gxp.pdf) [![Ensuring Regulatory Compliance: The Role of Validation in Pharmaceutical and Biotech Industries](https://www.moleculardevices.com/newsroom/in-the-news/media_131b1074acce1365c376e82a0c145217e0612fbaf.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/ensuring-regulatory-compliance-role-of-validation-in-pharmaceutical-and-biotech-industries) Publication Dec 10, 2023 ### [Ensuring Regulatory Compliance: The Role of Validation in Pharmaceutical and Biotech Industries](https://www.moleculardevices.com/newsroom/in-the-news/ensuring-regulatory-compliance-role-of-validation-in-pharmaceutical-and-biotech-industries) In this article, we discuss validation in the pharmaceutical and… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/ensuring-regulatory-compliance-role-of-validation-in-pharmaceutical-and-biotech-industries) [![GxP-Regulated Industry Assessments of Microplate Readers](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_1f309391753e8b132a531691fb798906a0ccc3e66.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-microplate-readers) Blog Jul 16, 2023 ### [GxP Regulated Industry Assessments of Microplate Readers](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-microplate-readers) This document details the references to 21 CFR Parts 58, 211, and 820,… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-microplate-readers) [![GxP-Regulated Industry Assessments of SoftMax Pro Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_19da1e5e31552e4124a39e364d8148ab926a835b0.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-softmax-pro-software) Blog Jul 16, 2023 ### [GxP regulated industry assessments of SoftMax Pro Software](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-softmax-pro-software) Discover how SoftMax Pro GxP Software meets FDA 21 CFR Part 11 and… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/gxp-regulated-industry-assessments-of-softmax-pro-software) [![FDA Fines & Warning Letters: How GxP Software Avoid Them](https://www.moleculardevices.com/newsroom/in-the-news/media_1a39daf9093236b5bed007525ed93f5747f1bc45d.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/millions-in-fda-fines-and-thousands-of-warning-letters-how-gxp-compliance-software-can-help) Publication Dec 18, 2022 ### [Millions in FDA Fines and Thousands of Warning Letters: How GxP Compliance Software Can Help Avoid Them](https://www.moleculardevices.com/newsroom/in-the-news/millions-in-fda-fines-and-thousands-of-warning-letters-how-gxp-compliance-software-can-help) Tim Bolus of Molecular Devices discusses how GxP compliance software… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/millions-in-fda-fines-and-thousands-of-warning-letters-how-gxp-compliance-software-can-help) [![Tech Blast | Quality assurance in drug discovery](https://www.moleculardevices.com/newsroom/in-the-news/media_1afafa5f83011a22a46b14db9009918587e3a82e1.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) Publication Dec 15, 2021 ### [Tech Blast \| Quality assurance in drug discovery](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) In this Tech Blast episode, Tim Bolus from Molecular Devices explores the… [Learn more](https://www.moleculardevices.com/newsroom/in-the-news/quality-assurance-in-drug-discovery) [![FDA 21 CFR Part 11: Ensuring Regulatory Compliance in GMP & GLP Labs](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_184e0cde7872849688a9c5ccef93ca80c21c136c5.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) Blog May 09, 2021 ### [FDA 21 CFR Part 11 and the importance of regulatory compliance in GMP and GLP labs](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) Know the importance of FDA 21 CFR Part 11 compliance in GMP and GLP labs.… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/fda-21-cfr-part-11-and-importance-of-regulatory-compliance-in-gmp-glp-labs) [![FUJIFILM Bacterial Endotoxin Assays on SpectraMax Readers](https://www.moleculardevices.com/products/gxp-compliance-solutions/media_124bed76591c3553e70912a62d119fd06b1228334.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) Application Note Jan 07, 2025 ### [FUJIFILM bacterial endotoxin assays on SpectraMax readers](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) Discover how FUJIFILM bacterial endotoxin assays on SpectraMax readers… [Learn more](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) [![Software Validation Test Suite: Operational Qualification (OQ) & Performance Qualification (PQ)](https://www.moleculardevices.com/images/default-card-thumbnail.webp?width=800&format=webp&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) Brochure Sep 17, 2024 ### [Software Validation Test Suite: Operational Qualification (OQ) & Performance Qualification (PQ)](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) Molecular Devices provides validation services for SoftMax® Pro GxP Data… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/software-validation-test-suite-oq-and-pq.pdf) **[View all Resources](https://www.moleculardevices.com/products/gxp-compliance-solutions/iq-oq-pm-services#resources "View all Resources")** ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=iq-oq-pm-services "Speak to a specialist")** ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Resources of IQ/OQ/PM Services [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products & Services of IQ/OQ/PM Services [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## 3D Cell Models in Drug Discovery ![3D Cell Models Will Shape the Future of Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/media_15c5efcd4dc74ffcccd8ef7729912e7ac33884bb2.jpeg?width=1200&format=jpeg&optimize=medium) # How 3D Cell Models Will Shape the Future of Drug Discovery March 07, 2023 Guest bloggers: Kenneth Pryde, Florian Fuchs, and Shan Dhamija Target discovery and drug development rely heavily on 2D cell and animal models to decipher efficacy and toxic effect of drug candidates. Yet, 90% of candidates fail to make it past phase III clinical trials (1). This is often due to complications that preclinical models could not capture. Upgrading these models can circumvent the risk of clinical failure and prevent potential delays or termination of drug development projects. Replacing 2D cell models with self-organizing 3D models is one step towards this goal. However, this is not a simple process, and it introduces as many challenges as added benefits. Drug Target Review discusses the past, present, and future of 3D cell models with [Kenneth Pryde](https://www.linkedin.com/in/kenneth-pryde/ "Kenneth Pryde"), Associate Director of Oncology Safety Sciences at AstraZeneca, [Florian Fuchs](https://www.linkedin.com/in/florianfuchs01/ "Florian Fuchs"), Chief Technology Officer at HeartBeat.bio, and [Shan Dhamija,](https://www.linkedin.com/in/shan-d-1965982/ "Shan Dhamija,") Vice President of Strategy and Innovation at Molecular Devices. _How 3D biology is shaping the future of drug discovery_ _Play Video_ _![](https://play.vidyard.com/kfyFLgxVTWuQbgiTnZN2x4.jpg)_ _In this roundtable discussion, leading experts from the industry discuss the promise of 3D biology and organoids for advancing drug discovery. They examine how to overcome challenges involved in 3D biology and what the future of organoid research will look like._ ### Are 2D cell cultures and animal models ideal during drug development? Animal models are widely used for human biology studies and drug development. From a regulatory perspective and safety perspective, it is necessary for a drug molecule to undergo preclinical animal trials before phase 1 clinical trials. However, these preclinical observations have not always translated to clinical performance, due to limited assessment of pharmacokinetics and pharmacodynamics in animals as compared to humans. Dr. Pryde emphasizes the need for in vitro models: “Additional in vitro models are required to complement those animal models to help decrease the risk of attrition and safety issues manifesting in the clinic, which the animal models were not able to identify.” "Additional in vitro models are required to complement those animal models to help decrease the risk of attrition and safety issues manifesting in the clinic, which the animal models were not able to identify." Although 2D cellular models complement animal studies and improve preclinical assessment accuracy, their limitations surface with increasingly complex disease models and targeted drug discovery. Traditional 2D cell models and corresponding assays fail to recapitulate physiological events, such as cell-cell communication, cell-extracellular matrix (ECM) interactions, and physical constraints. According to Mr. Dhamija, a focus on new technologies is disproportionate: “While new tools allow precise and nuanced discovery at greater biological depth unless we change the model systems, we will only get better at finding the wrong answers.” "While new tools allow precise and nuanced discovery at greater biological depth unless we change the model systems, we will only get better at finding the wrong answers." ### How do 3D cell models prevail over 2D cell models? One of the main reasons [3D cell models](https://www.moleculardevices.com/applications/3d-cell-models "3D cell models") stand out is that in contrast to 2D cell models, they can better represent the in vivo environment, making them more predictive of the potential effects of a compound. This also helps them perform better in toxicology studies, as they can preclinically predict potential risks. From this perspective, 3D cell models mitigate the risks associated with false negatives or positives in animal studies. Since animal models are still indispensable in drug development and testing, 3D cell models can also be utilized to assess their relevance to human physiology. Cell models representing different animal tissues can help elucidate the correlation between animal and human cellular dynamics affecting immunogenic responses and functional alterations. The combination of types of models results in a more systemic view of the tissue or organ studied while minimizing the risk of data misinterpretation. The combination of types of models results in a more systemic view of the tissue or organ studied while minimizing the risk of data misinterpretation. ### Different types of 3D cell models and their advantages We can categorize 3D cell models into four main groups - spheroids, 3D bioprinted models, organoids, and organ-on-a-chip. Spheroids consist of a group of cells coalesced together to form spheres. 3D bioprinted models are a step above, as they introduce spatial complexity or extracellular matrices in addition to the cells. Organoids are tiny, self-organized three-dimensional tissue cultures that are derived from stem cells. The final category is organ-on-a-chip which implements microfluidics to simulate circulation, mechanical stressors, and the impact of interactions between different tissues. ![Examples of 3D cell models - Spheroids, patient-derived organoids, cardioid (heart organoid), and lung organoids](https://www.moleculardevices.com/lab-notes/3d-biology/media_1fef0df219ccebb213e1845a3f0e14c558d8cec66.jpeg?width=750&format=jpeg&optimize=medium) _Examples of 3D cell models from the top left - Spheroids, patient-derived organoids (or tumoroids), cardioid (heart organoid), and lung organoids._ Each type of 3D model has its own advantages and disadvantages. However, organoids are widely used for a number of reasons. Dr. Fuchs favors organoids over other 3D models because “they have intrinsic capabilities for amplification, allowing the modeling of all developmental steps in a tissue”. However, he does acknowledge the limitations, such as the lack of an immunogenic component. Another highlight of organoids and spheroids, as emphasized by Dr. Pryde, is their scalability and high-throughput capability, as both are feasible for 384-well plates. This allows them to be regularly employed in high-throughput drug screening for efficacy and toxicology studies. One of the main advantages of organoids is their versatility. One can tweak the organoid design to render a specific function through a huge repertoire of tools. Pluripotent stem cells are the primary source in organoid studies, as they can directly differentiate into tissue-specific cells. This makes it more biologically relevant and better at recapitulating the constituent cell types and extracellular matrix. Furthermore, the fact that these can be genetically altered enables the acquisition of deeper insights when studying disease mechanisms, drug action, drug resistance, and toxicology. ![Mimeta’s organ-on-a-chip technology, OrganoPlate](https://www.moleculardevices.com/lab-notes/3d-biology/media_10a3d015c065b4f673c161cfb7f78d1272706585d.png?width=750&format=png&optimize=medium) _Mimeta’s organ-on-a-chip technology, OrganoPlate®_ That said, other models like organ-on-an-a-chip can recapitulate more sophisticated systems despite a lower throughput. At that point, it is up to the researchers to decide the relative importance of complexity over high throughput to select the optimum model for their specific research. ### Future of organoid research The emergence of biologically relevant organoids and technological refinements in organoid workflows create space for exciting research opportunities. Dr. Fuchs emphasizes the possible use of synthetic embryos to mimic embryo development, which could drive a better understanding of human development. He also addresses the need to transition to a completely animal-free preclinical testing routine, calling on regulatory parties to readjust their criteria. “In fact, animal models may not even be useful in testing biologics, such as vaccines for antibodies that are so humanized that they are weakly immunogenic in animal models. So, it does not make sense for us to use animals knowing that we will not be observing the impact of the therapeutic.” "In fact, animal models may not even be useful in testing biologics, such as vaccines for antibodies that are so humanized that they are weakly immunogenic in animal models. So, it does not make sense for us to use animals knowing that we will not be observing the impact of the therapeutic." Dr. Pryde anticipates that organoid research can immensely contribute to personalized medicine. “3D models create this new avenue in the therapeutic space around accessing biological material from patients to identify potential drug sensitivity or resistance. The results of this analysis can inform clinicians to decide whether a particular drug is beneficial and suitable for a patient or not”. According to Mr. Dhamija, machine learning is likely to become a more integral part of 3D models, especially when derisking model systems. “While in academia, the incentive is for pursuing increased complexity, establishing robust 3D model research protocols is just as important for the industry. With machine learning, novel models can be made more reproducible, scalable, and consistent, which mitigates risk of data discrepancies that would cause significant delays in delivery to market and patients." With machine learning, novel models can be made more reproducible, scalable, and consistent, which mitigates risk of data discrepancies that would cause significant delays in delivery to market and patients. Nevertheless, [organoid research](https://www.moleculardevices.com/applications/organoid-innovation-center "organoid research") has a long path to perfection. Of course, one of the challenges is gaining access to large patient samples or finding sufficient numbers of stem cell donors. Currently, drug screening and [toxicology](https://www.moleculardevices.com/applications/toxicology "toxicology") studies fail to translate to clinical studies because of patient variability. That’s why access to patient-derived material needs to be broadened to account for these variations in preclinical settings. Furthermore, to improve the credibility of predictions, researchers can complement _in vitro_ models with computational models, which also brings a more holistic outlook to the disease of interest. 1. Mullard, Asher. "Parsing clinical success rates." Nature Reviews Drug Discovery 15.7 (2016): 447-448. \\* end \* ### Take a peek into where our research happens! Tour our [Organoid Innovation Center](https://www.moleculardevices.com/applications/organoid-innovation-center?cmp=7014u000001RIOWAA4&utm_source=internal&utm_medium=email&utm_campaign=md-na-cc-eb-internal-20220602-isscr2022 "Organoid Innovation Center") where we showcase cutting-edge instruments that work harmoniously together for autonomous, long-term, live cell 2D and 3D cell culture growth and monitoring with intelligent label-free imaging. This integrated workflow provides quality control alerts and readiness, 3D organoid screening, and deep-learning image analysis that reveals hidden patterns other technologies miss. #### Recent posts [![A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/3d-biology/media_135347bfb9e0627730d814fb2b96129223194220b.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) ### [A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) Explore the origins of patch-clamp electrophysiology—foundational… [Create history with AXON](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) [![The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/lab-notes/3d-biology/media_1fae38c1022f68e210e4bf36e29c7c1b648734fcc.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) ### [The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) Dr. Misha Bashkurov shares insights on how AI is transforming life science… [Read the blog](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) [![3D Organoids vs 2D Cell Lines in Drug Discovery & Disease Modeling](https://www.moleculardevices.com/lab-notes/3d-biology/media_1d395cfab0af9a8962c32d1f3331c0e00ae261c50.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) ### [3D Organoids vs 2D Cell Lines in Drug Discovery and Disease Modeling](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) Explore the benefits and challenges of 3D organoids vs 2D cell lines in… [Read the blog](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) [![Organoids Accelerate Parkinson’s and Alzheimer’s Research & Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/media_169de53d0a9446dbfd6eff54bd7a2b801ba916f4d.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) ### [Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) Explore how 3D brain organoids from hiPSCs transform neurodegenerative… [Read the blog](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) [![A Brief History of Patch-Clamping — Part 2: Building the Instrumentation](https://www.moleculardevices.com/lab-notes/3d-biology/media_19218865f6633d4c73216a35bae100c778620bfa0.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-2) ### [A Brief History of Patch-Clamping — Part 2: Building the Instrumentation](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-2) Explore the evolution of patch-clamp electrophysiology from manual… [Create history with AXON](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-2) [![Compliance Made Simple: How GxP Solutions Are Changing Drug Development](https://www.moleculardevices.com/lab-notes/3d-biology/media_16b177185577a837ff4463907ed88773498089d3e.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/how-gxp-solutions-are-changing-drug-development) ### [Compliance Made Simple: How GxP Solutions Are Changing Drug Development](https://www.moleculardevices.com/lab-notes/microplate-readers/how-gxp-solutions-are-changing-drug-development) This Insider Insights series explores how GxP solutions simplify… [Read the blog](https://www.moleculardevices.com/lab-notes/microplate-readers/how-gxp-solutions-are-changing-drug-development) [![A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/3d-biology/media_135347bfb9e0627730d814fb2b96129223194220b.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) ### [A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) Explore the origins of patch-clamp electrophysiology—foundational… [Create history with AXON](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) [![The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/lab-notes/3d-biology/media_1fae38c1022f68e210e4bf36e29c7c1b648734fcc.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) ### [The Transformative Power of AI-Enabled Data Analytics](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) Dr. Misha Bashkurov shares insights on how AI is transforming life science… [Read the blog](https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/transformative-power-of-ai-enabled-data-analytics) [![3D Organoids vs 2D Cell Lines in Drug Discovery & Disease Modeling](https://www.moleculardevices.com/lab-notes/3d-biology/media_1d395cfab0af9a8962c32d1f3331c0e00ae261c50.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) ### [3D Organoids vs 2D Cell Lines in Drug Discovery and Disease Modeling](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) Explore the benefits and challenges of 3D organoids vs 2D cell lines in… [Read the blog](https://www.moleculardevices.com/lab-notes/3d-biology/3d-organoids-vs-2d-cell-lines-drug-discovery) [![Organoids Accelerate Parkinson’s and Alzheimer’s Research & Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/media_169de53d0a9446dbfd6eff54bd7a2b801ba916f4d.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) ### [Organoids Promise to Accelerate Parkinson's and Alzheimer’s Research and Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) Explore how 3D brain organoids from hiPSCs transform neurodegenerative… [Read the blog](https://www.moleculardevices.com/lab-notes/3d-biology/organoids-accelerate-parkinsons-and-alzheimers-research-and-drug-discovery) ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## ImageXpress Micro Confocal ![ImageXpress Micro Confocal](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1874f056e22b41d21b6173821d5655abd5803e8a9.jpg?width=1200&format=jpg&optimize=medium) # ImageXpress Micro Confocal High-Content Imaging System ![ImageXpress Micro Confocal High-Content Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_14a93d9df01a4205e88cec164fd810cd1754fe95e.png?width=750&format=png&optimize=medium) Unique confocal imaging solution capable of imaging more than a million wells a week **[View Cell Gallery](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal# "View Cell Gallery")** _[Download eBook](https://www.moleculardevices.com/en/assets/ebook/dd/img/3d-cellular-imaging "Download eBook")_ ![](https://www.moleculardevices.com/images/play_icon.png) - [Overview](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#overview) - [Options](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#options) - [Applications](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) - [Specifications & Options](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#specifications-options) - [Resources](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#resources) - [Citations](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#citations) - [Related Products & Services](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#related-products-services) ## High-content confocal imaging solution with water objective options The ImageXpress® Micro Confocal system is a high-content solution that can switch between widefield and confocal imaging of fixed and live cells. It can capture high quality images of whole organisms, thick tissues, 2D and 3D models, and cellular or intracellular events. The spinning disk confocal and sCMOS camera enable imaging of fast and rare events like cardiac cell beating and stem cell differentiation. With [MetaXpress](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/metaxpress "MetaXpress") software and flexible options like water immersion objectives to choose from, the system enables many confocal imaging applications from 3D assay development to screening. ![Images Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d18598231e64c1edc1e6abaac9eaaf12cf09e6a9.png?width=750&format=png&optimize=medium) ### Acquire higher quality images Capture excellent contrast, high resolution images with our proprietary AgileOptix™ spinning disk confocal technology, wide field of view, and bright light source. ![Customizable Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1fe5bdcf153d4321fd43b86bc823b8fb1d1e0a5bd.png?width=750&format=png&optimize=medium) ### Customize image acquisition and analysis Take ultimate control over acquisition and analysis parameters, which enables many applications from 3D structure analysis to target imaging of specific objects within an organism or cell populations. Run more applications with standard options like water immersion objectives, environmental control, or any of our high-performance customizations. ![Analysis Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1af4b258e3eb2fdd828db8a53de70ba185fc96342.png?width=750&format=png&optimize=medium) ### Analyze more data in less time MetaXpress® PowerCore™ software accelerates analysis speed in a high-throughput environment. The software distributes image processing jobs to a multi-CPU environment. ![](https://play.vidyard.com/24FeCTPpMYqMJUCLauSFTD.jpg) ImageXpress Micro Confocal Virtual Tour ## Features ![Breadth Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d79aa39e841abb3316eee06494a1a6249e478050.png?width=750&format=png&optimize=medium) ### Wide dynamic range Quantify low and high intensity signals in a single image with >3 log dynamic range intensity detection. ![Optics Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d46908a4d9a7b3f59bb353c7c9a6941e048e2488.png?width=750&format=png&optimize=medium) ### Exclusive AgileOptix spinning disk confocal The technology provides increased sensitivity with specially designed optics, high-powered, solid state light engines, and sCMOS sensor. Swappable disc geometries provide flexibility between speed and resolution. ![Accuracy Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1ac46e976bc1583af36e051690cd93abd163f8733.png?width=750&format=png&optimize=medium) ### Wide field of view Wide field of view enables whole-well confocal imaging and eliminates missed targets. ![Automation Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_156d62195b24e34de4cd0c2506768b3fab13a5980.png?width=750&format=png&optimize=medium) ### Optional on-board robotic fluidics For assays that involve compound addition, well washing, and media exchange, optional on-board robotic fluidics are available. ![Measure Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_150ce21c654344a33ffe48acc035b49d76ca3071d.png?width=750&format=png&optimize=medium) ### Accurate 3D measurements MetaXpress 3D analysis module is optimized for confocal imaging, enabling 3D measurements of volume and distance. ![Expandable Icon](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1124a9c8fc6f2d78b1ee36fff51fdaa77c8d64c5b.png?width=750&format=png&optimize=medium) ### Multiple imaging modes The system offers phase contrast and brightfield label-free imaging, fluorescence, widefield, colorimetric, and confocal imaging, as well as, water immersion imaging as a standard option. ## AgileOptix™ technology Our proprietary AgileOptix technology enables the ImageXpress Micro Confocal system to deliver the sensitivity and throughput needed for demanding applications. AgileOptix is the combination of a powerful solid state, light engine, specially designed optics, scientific CMOS sensor, and the ability to change disk geometries. ![AgileOptix Technology for ImageXpress Micro Confocal](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_16f2ea1276b9261656c44fb578f4c37245f358b4f.jpg?width=750&format=jpg&optimize=medium) **[View options](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#options "View options")** ## MetaXpress software for ImageXpress high-content imaging systems #### Multi-level analysis tools for a wide range of applications [![MetaXpress software high-content imaging systems](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_156eb21094c33c9152de24291b0ba89bb09fdd02d.png?width=750&format=png&optimize=medium)](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/metaxpress "https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/metaxpress") - Meet high throughput requirements with a scalable, streamlined workflow - Adapt your analysis tools to tackle your toughest problems, including 3D analysis - Schedule automatic data transfer between third-party hardware sources and secure database - Set up hundreds of routinely used HCS assays using MetaXpress software modules - Export data to IN Carta software, leveraging intuitive, modern machine learning _[View Modules](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/metaxpress#Applications "View Modules")_ **[Learn more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/metaxpress "Learn more")** ## Cellular Image Gallery ![Micro Confocal High-Content Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_12b290d3a4281c7fe9cb9fd08c4f12d89a4e73b49.jpg?width=750&format=jpg&optimize=medium) ![ImageXpress High-content imaging solution](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_143f7c0fc62caadf133fee3f285191d4847219e3b.jpg?width=750&format=jpg&optimize=medium) ![ImageXpress Micro Confocal Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_11fe35582bbb4c4469b8529a5f992572b61718992.jpg?width=750&format=jpg&optimize=medium) ![ImageXpress Micro Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_135c5c453978cd35d964e462fe4162e404b1678db.jpg?width=750&format=jpg&optimize=medium) ![Micro Confocal Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1cfa97d1ec40d36f2ef337b8f2f0d3c7c1bf317e9.jpg?width=750&format=jpg&optimize=medium) ###### **†** Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. ## Latest Resources [![AI-enabled hit selection of drug screening on human pancreatic cancer organoids](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1354ca4d8fff7ae09015059583d513264d5c785ec.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) Application Note Nov 19, 2024 ### [AI-enabled hit selection of drug screening on human pancreatic cancer organoids](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) We exposed human pancreatic cancer PDOs to anti-cancer compounds at… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) [![A microwell platform to standardize human rectal organoid cultures for high-content imaging and phenotypic analyses](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1938aad75df0577f5dd25f56d025598e765832300.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) Application Note Nov 18, 2024 ### [A microwell platform to standardize human rectal organoid cultures for high-content imaging and phenotypic analyses](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) Learn how Gri3D technology helps overcome challenges in human rectal… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) [![Automated Culture Analysis of Compound Responses in Human iPSC-Derived 3D tri-culture Cardiac Spheroids](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1c41fcaeff155b5292cd158e1f56db80c5c01f303.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) Application Note Apr 10, 2025 ### [Automated culture and functional analysis of compound responses in human iPSC-derived 3D tri-culture cardiac spheroids](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) Learn how an automated workflow using CellXpress.ai® and FLIPR® Penta… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) [![Photoreceptor Functional Activity in Retinal Organoids using Calcium Imaging](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_135e8deb095ee95959b7d17063357d6fd67dcf11a.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) Application Note Apr 03, 2025 ### [Assessment of photoreceptor functional activity in retinal organoids using calcium imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) Discover a high-content imaging method to assess photoreceptor… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) [![Functional analysis of spontaneous calcium oscillations of iPSC-derived 3D neural organoids and responses to neuroactive compounds](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d8e2d37088f59c9d9736740e315e4230a23a783d.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/flipr/functional-analysis-of-spontaneous-calcium-oscillations-of-ipsc-derived-3d-neural-organoids-and-neuroactive-compounds) Application Note Feb 06, 2025 ### [Functional analysis of spontaneous calcium oscillations of iPSC-derived 3D neural organoids and evaluation of responses to neuroactive compounds](https://www.moleculardevices.com/en/assets/app-note/dd/flipr/functional-analysis-of-spontaneous-calcium-oscillations-of-ipsc-derived-3d-neural-organoids-and-neuroactive-compounds) This app note highlights the use of 3D neural organoids from human… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/flipr/functional-analysis-of-spontaneous-calcium-oscillations-of-ipsc-derived-3d-neural-organoids-and-neuroactive-compounds) [![Assay Kits, Reagents, and Consumables](https://www.moleculardevices.com/sites/default/files/2025/assay-kits-reagents-and-consumables.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/reagents/assay-kits-reagents-and-consumables.pdf) Brochure Feb 04, 2025 ### [Assay Kits, Reagents, and Consumables](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/reagents/assay-kits-reagents-and-consumables.pdf) Unlock the full potential of your research with our optimized and… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/reagents/assay-kits-reagents-and-consumables.pdf) [![Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1e10a63a7266b3a8a7f6725d51bf0cbf8dc9dcae9.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) News Jan 15, 2025 ### [Molecular Devices Fast-Tracks High-Quality Imaging and Analysis with New ImageXpress HCS.ai High-Content Screening System](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) Molecular Devices Introduces the ImageXpress HCS.ai High-Content Screening… [Learn more](https://www.moleculardevices.com/newsroom/news/high-quality-imaging-and-analysis-with-imagexpress-hcs-ai-system) [![3D Biology: The paradigm shift in next-generation drug discovery [Interactive infographic]](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1c761770b532a641e8ebb023c3aebc9edda9ae595.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Blog Jan 07, 2025 ### [3D Biology: The Paradigm Shift in Next-Gen Drug Discovery](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) Discover how 3D biology is revolutionizing drug discovery by providing… [Learn more](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery) [![T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/2024/t-cell-induced-morphological-analysis-of-colorectal-cancer-organoids-using-ai.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) Scientific Poster Nov 20, 2024 ### [T cell-induced morphological change analysis of colorectal cancer organoids using AI](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) To overcome the challenges of using PDOs in large-scale applications, we… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/dd/img/t-cell-induced-morphological-change-analysis-of-colorectal-cancer-organoids-using-ai.pdf) [![AI-enabled hit selection of drug screening on human pancreatic cancer organoids](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1354ca4d8fff7ae09015059583d513264d5c785ec.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) Application Note Nov 19, 2024 ### [AI-enabled hit selection of drug screening on human pancreatic cancer organoids](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) We exposed human pancreatic cancer PDOs to anti-cancer compounds at… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/ai-enabled-drug-screening-on-human-pancreatic-cancer-organoids) [![A microwell platform to standardize human rectal organoid cultures for high-content imaging and phenotypic analyses](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1938aad75df0577f5dd25f56d025598e765832300.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) Application Note Nov 18, 2024 ### [A microwell platform to standardize human rectal organoid cultures for high-content imaging and phenotypic analyses](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) Learn how Gri3D technology helps overcome challenges in human rectal… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/microwell-platform-to-standardize-human-rectal-organoid-cultures-for-imaging-and-phenotypic-analyses) [![Automated Culture Analysis of Compound Responses in Human iPSC-Derived 3D tri-culture Cardiac Spheroids](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1c41fcaeff155b5292cd158e1f56db80c5c01f303.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) Application Note Apr 10, 2025 ### [Automated culture and functional analysis of compound responses in human iPSC-derived 3D tri-culture cardiac spheroids](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) Learn how an automated workflow using CellXpress.ai® and FLIPR® Penta… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/automated-culture-analysis-of-human-ipsc-derived-3d-tri-culture-cardiac-spheroids) [![Photoreceptor Functional Activity in Retinal Organoids using Calcium Imaging](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_135e8deb095ee95959b7d17063357d6fd67dcf11a.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) Application Note Apr 03, 2025 ### [Assessment of photoreceptor functional activity in retinal organoids using calcium imaging](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) Discover a high-content imaging method to assess photoreceptor… [Learn more](https://www.moleculardevices.com/en/assets/app-note/dd/img/photoreceptor-functional-activity-in-retinal-organoids-using-calcium-imaging) **[View all Resources](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#resources "View all Resources")** ## Featured Applications ![Pluripotent stem cells](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1a2ea9f9d0bbca920f2ce9acc926e39519683ed42.png?width=750&format=png&optimize=medium) ### Stem Cell Research Pluripotent stem cells can be used for studies in developmental biology or… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Toxicology](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1e152cdc56207076284ab30a2a9be334b108a627d.jpeg?width=750&format=jpeg&optimize=medium) ### Toxicology Toxicology is the study of adverse effects of natural or man-made… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![3D Cell Imaging and Analysis](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_102fdfc2b411108b3f001876a686434b43538f9b6.png?width=750&format=png&optimize=medium) ### 3D Cell Imaging and Analysis Three-dimensional (3D) cell models are physiologically relevant and more… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![SARS-CoV-2 virus](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1c32fa83ec1c63dacfa00123484a483a698092148.jpeg?width=750&format=jpeg&optimize=medium) ### COVID-19 and Infectious Disease Research Here we've addressed common applications in infectious disease research… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Cell Counting](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_13505877bf1900eb74e1de71aa3f2759a48095e4b.jpeg?width=750&format=jpeg&optimize=medium) ### Cell Counting Cell counting is fundamental and critical to numerous biological… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Cell Migration Assays](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_19996188f1ad852cd11ee845a893b0e4c52196f9b.png?width=750&format=png&optimize=medium) ### Cell Migration Assays The movement or migration of cells is often measured in vitro to elucidate… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Cells are grown in an extracellular matrix (ECM) to mimic an in vivo environment](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1e7c6a6c1bdaba7f2cb70e6456a9140236fcf90b2.jpeg?width=750&format=jpeg&optimize=medium) ### Cells in Extracellular Matrices One common way of culturing cells in three dimensional space is through… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Disease Modeling](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_14643b36b5b24607050217e08b5d72ef3881017e2.jpeg?width=750&format=jpeg&optimize=medium) ### Disease Modeling Disease model systems range in complexity and scale from simple 2D cell… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Drug Discovery & Development Process](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1fea24937c7c28125b621f35b26badeb34d6d9ddc.jpeg?width=750&format=jpeg&optimize=medium) ### Drug Discovery & Development For every drug that makes it to the finish line, another nine don’t… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Live cell imaging](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_14cae6986a10eb5c77271e69e00322de4d377f9ba.png?width=750&format=png&optimize=medium) ### Live cell imaging Live cell imaging is the study of cellular structure and function in… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Neurite Outgrowth / Neurite Tracing](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1800096c2c7be51a59fa3956f5e9f426699aa60ff.jpeg?width=750&format=jpeg&optimize=medium) ### Neurite Outgrowth / Neurite Tracing Neurons create connections via extensions of their cellular body called… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Oncology - Cancer Research](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_133eafed6bd11826e11f637ad8986815fbafccd1e.jpeg?width=750&format=jpeg&optimize=medium) ### Oncology - Cancer Research Cancer researchers need tools that enable them to more easily study the… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Organ-on-a-Chip](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_13da64333f0c556103f80e00f863a6396051f6141.jpeg?width=750&format=jpeg&optimize=medium) ### Organ-on-a-Chip Organ-on-a-chip (OoC) is a technology that uses microfabrication… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Spheroids](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1dcd04ebd4aff3533213b39380908f9e0121cf061.jpeg?width=750&format=jpeg&optimize=medium) ### Spheroids Spheroids are multi-cellular 3D structures that mimic in vivo cell… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Pluripotent stem cells](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1a2ea9f9d0bbca920f2ce9acc926e39519683ed42.png?width=750&format=png&optimize=medium) ### Stem Cell Research Pluripotent stem cells can be used for studies in developmental biology or… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![Toxicology](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1e152cdc56207076284ab30a2a9be334b108a627d.jpeg?width=750&format=jpeg&optimize=medium) ### Toxicology Toxicology is the study of adverse effects of natural or man-made… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![3D Cell Imaging and Analysis](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_102fdfc2b411108b3f001876a686434b43538f9b6.png?width=750&format=png&optimize=medium) ### 3D Cell Imaging and Analysis Three-dimensional (3D) cell models are physiologically relevant and more… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ![SARS-CoV-2 virus](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1c32fa83ec1c63dacfa00123484a483a698092148.jpeg?width=750&format=jpeg&optimize=medium) ### COVID-19 and Infectious Disease Research Here we've addressed common applications in infectious disease research… [Read more](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/imagexpress-micro-confocal#applications) ## Customer Breakthrough ![NAFLD disease progression using ImageXpress Micro Confocal](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d7dc85ee87650cb9fdc0a2d02e3a22d557b0d4cc.jpg?width=750&format=jpg&optimize=medium) SUCCESS STORY ### Visikol team develops 3D cell culture models to simulate the NASH and NAFLD disease progression process using ImageXpress Micro Confocal High-Content Imaging System and MetaXpress software [Continue Reading](https://www.moleculardevices.com/en/assets/customer-breakthrough/dd/img/visikol "Continue Reading") ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=imagexpress-micro-confocal-high-content-imaging-system "Speak to a specialist")** ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Expand your research using flexible, high-performance imaging solutions Molecular Devices provides flexible options for the ImageXpress Micro Confocal High-Content Imaging System to meet your research needs and to easily capture images from different sample formats, including hanging drops and in round or flat bottom plates, for monitoring cell health kinetics under environmental control, and more. With over 30+ years of imaging expertise, we can help you select the right options to ensure the best images for your assay. ## Standard hardware options ![Water immersion objectives](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_12632add7bbcc144bd652cfaa2c83179521a1a50c.png?width=750&format=png&optimize=medium) ### Water immersion objectives 20X, 40X, and 60X [water immersion objectives](https://www.moleculardevices.com/technology/water-immersion-objectives "water immersion objectives") improve the geometric accuracy during acquisition and reduce light refraction for brighter intensity at lower exposure times. ![Phrase Contrast](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1b5a7034fc436aee407e064db17681c8d8865c3b6.png?width=750&format=png&optimize=medium) ### Transmitted light tower Our transmitted light tower enables acquisition of high contrast images for unstained cells which can be easily viewed or separated from background. ![urnkey, high-throughput long term kinetics](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_144510756fb1c5f9d3086aeb0598bbed5cfb80b82.png?width=750&format=png&optimize=medium) ### Environmental Control Environmental control maintains temperature and humidity levels while minimizing evaporation for multi-day, live cell, time-lapse imaging. ![Robotic Automation](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_107c497da7dde99ccc12aa19c117c24c34bf65dbb.png?width=750&format=png&optimize=medium) ### On-board robotic fluidics Integrated fluidics automates assay workflows which require compound addition, well washing, and media exchange. ## Customization options Molecular Devices can successfully tailor the ImageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described below, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a [fully automated workcell](https://www.moleculardevices.com/service-support/lab-automation-solutions "fully automated workcell"). With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support. Sale is subject to our Custom Product Purchase Terms available at [www.moleculardevices.com/custom-products-purchase-terms](https://www.moleculardevices.com/custom-products-purchase-terms "www.moleculardevices.com/custom-products-purchase-terms") ![High-Intensity lasers](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_102e220f01f2c58a86a170e232372ec6f0e6eac23.png?width=750&format=png&optimize=medium) ### High-Intensity lasers Expand experiment capabilities with 5- or 7-channel high-intensity lasers. ![Automatic Pipettor](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_130d3a1ee343d406e5f18f8e39b1b3b05a1b30408.png?width=750&format=png&optimize=medium) ### Real-time dose response Automatic pipettor enables compound addition while simultaneously live streaming at >100 frames per second. ![Confocal Disk Module](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_13245411d825dbc4d4497b5ec5b26c9a3fa2909a1.png?width=750&format=png&optimize=medium) ### Deep tissue penetrating confocal disk module The deep tissue penetrating, confocal disk module reduces crosstalk to improve out-of-focus light suppression and penetrate deeper into tissue. ![Turnkey, high-throughput long term kinetics](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_181523807fcf040e56b27583025eba7645a46788a.png?width=750&format=png&optimize=medium) ### Turnkey, High-throughput long term kinetics Schedule and image multiple plates over long periods of time while keeping consistent temperature, O2(Hypoxia), CO2, and humidity conditions. Expand live cell walk-away capacity to 200+ plates. ![Robotic Automation](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_150a0b710ca6d55dfc0c86bf57e03986460cb06bf.png?width=750&format=png&optimize=medium) ### Scale up robotic automation Increase throughput, eliminate human errors, maintain sterility, and achieve consistent sample handling. Modular automation design—components can be added in modules and are upgradeable. **[Download brochure](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/high-performance-customizations.pdf "Download brochure")** ![High Intensity Lasers](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_102e220f01f2c58a86a170e232372ec6f0e6eac23.png?width=750&format=png&optimize=medium) ## High-intensity Lasers High output laser excitation can reduce exposure times by up to 75%.† The laser light source is available as either a 5-channel or 7-channel light source with outputs of 400 —1,000 mW/channel. The 7-channel laser light source Includes near IR and is ideal for customers with increased multiplexing requirements. - Obtain sharper images with higher signal-to-noise - Generate up to a 2X† boost in scan speed attributed to significantly reduced exposure times - Run FRET experiments using lasers for CFP and YFP **[Learn more](https://www.moleculardevices.com/technology/confocal-microscopy-lasers "Learn more")** Standard Laser ![Standard Intensity Image](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1d9f724cb4e5a4c5c1cdb97ef60c58ff661be31c8.png?width=750&format=png&optimize=medium) ![High-Intensity Lasers Image](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_12e0b1b724755c32afee20f985c7c5065e32f1476.png?width=750&format=png&optimize=medium) **Images taken at the same exposure.** ![Confocal Disk Module](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_13245411d825dbc4d4497b5ec5b26c9a3fa2909a1.png?width=750&format=png&optimize=medium) ## Deep Tissue Penetrating, Confocal Disk Module Specialized deep tissue penetrating, confocal disk module, combined with a laser light source, improves light penetrance for deeper tissue penetration, resulting in sharper images with improved resolution when imaging thick tissue samples†. - Improve suppression of out-of-focus light - Reduce haze (pinhole crosstalk) - Penetrate deeper into thick tissue samples for sharper images **[Learn more](https://www.moleculardevices.com/technology/agileoptix-spinning-disk-confocal "Learn more")** Standard spinning disk Deep tissue penetrating, confocal disk module ![Standard Spinning Disc](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_186daa41eb06bf113e92b7d2a3e56dd16e49e8818.png?width=750&format=png&optimize=medium) ![Deep Tissue Penetrating with Confocal Disk](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_145b8a69f22a442c7ed271b3d6b9f9391ca76460c.png?width=750&format=png&optimize=medium) **Images taken at the same exposure.** ###### †Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Applications of ImageXpress Micro Confocal High-Content Imaging System [3D Cell Imaging and Analysis](https://www.moleculardevices.com/fragments/applications/3d-cell-imaging-and-analysis "3D Cell Imaging and Analysis") [Cancer Research](https://www.moleculardevices.com/fragments/applications/cancer-research "Cancer Research") [Cell Counting](https://www.moleculardevices.com/fragments/applications/cell-counting "Cell Counting") [Cell Migration Assays](https://www.moleculardevices.com/fragments/applications/cell-migration-assays "Cell Migration Assays") [Cells in Extracellular Matrices](https://www.moleculardevices.com/fragments/applications/cells-in-extracellular-matrices "Cells in Extracellular Matrices") [COVID-19 and Infectious Disease Research](https://www.moleculardevices.com/fragments/applications/covid-19-and-infectious-disease-research "COVID-19 and Infectious Disease Research") [Drug Discovery & Development](https://www.moleculardevices.com/fragments/applications/drug-discovery-and-development "Drug Discovery & Development") [Live cell imaging](https://www.moleculardevices.com/fragments/applications/live-cell-imaging "Live cell imaging") [Neurite Outgrowth / Neurite Tracing](https://www.moleculardevices.com/fragments/applications/neurite-outgrowth-neurite-tracing "Neurite Outgrowth / Neurite Tracing") [Organ-on-a-Chip](https://www.moleculardevices.com/fragments/applications/organ-on-a-chip "Organ-on-a-Chip") [Spheroids](https://www.moleculardevices.com/fragments/applications/spheroids "Spheroids") [Stem Cell Research](https://www.moleculardevices.com/fragments/applications/stem-cell-research "Stem Cell Research") [Toxicology](https://www.moleculardevices.com/fragments/applications/toxicology "Toxicology") [Disease Modeling](https://www.moleculardevices.com/fragments/applications/disease-modeling-2 "Disease Modeling") [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Specifications & Options of ImageXpress Micro Confocal High-Content Imaging System [Specifications & Options of ImageXpress Micro Confocal High-Content Imaging System](https://www.moleculardevices.com/products/specifications/imagexpress-micro-confocal.json "Specifications & Options of ImageXpress Micro Confocal High-Content Imaging System") ### [Download Brochure](https://chat.moleculardevices.com/imaging-solutions-for-your-biological-questions "Download Brochure") ![download pdf](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_114e4e5ccf8efcad1115a9dc371921ff8e7dfae10.png?width=750&format=png&optimize=medium) [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Resources of ImageXpress Micro Confocal High-Content Imaging System [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products & Services of ImageXpress Micro Confocal High-Content Imaging System [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ![Micro Confocal High-Content Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_12b290d3a4281c7fe9cb9fd08c4f12d89a4e73b49.jpg?width=750&format=jpg&optimize=medium) Micro Confocal High-Content Imaging System ![ImageXpress High-content imaging solution](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_143f7c0fc62caadf133fee3f285191d4847219e3b.jpg?width=750&format=jpg&optimize=medium) ImageXpress High-content imaging solution ![ImageXpress Micro Confocal Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_11fe35582bbb4c4469b8529a5f992572b61718992.jpg?width=750&format=jpg&optimize=medium) ImageXpress Micro Confocal Imaging System ![ImageXpress Micro Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_135c5c453978cd35d964e462fe4162e404b1678db.jpg?width=750&format=jpg&optimize=medium) ImageXpress Micro Imaging System ![Micro Confocal Imaging System](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging/media_1cfa97d1ec40d36f2ef337b8f2f0d3c7c1bf317e9.jpg?width=750&format=jpg&optimize=medium) Micro Confocal Imaging System ## Consumer Rights Notice ![](https://www.moleculardevices.com/media_1b94b5d556ad08f0d9695d60f6bf50c340929476b.jpeg?width=1200&format=jpeg&optimize=medium) # California Consumer Rights Notice ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## 3D Biology Revolution ![3D Biology: next-generation drug discovery](https://www.moleculardevices.com/lab-notes/3d-biology/media_1eafa92e34441e4fc5cd3bc54c9cd05caa96c23e6.jpg?width=1200&format=jpg&optimize=medium) # 3D Biology: The paradigm shift in next-generation drug discovery \[Interactive infographic\] January 08, 2025 3D Biology Team _Originally Published: April 19, 2023_ ### The drug discovery landscape is changing Bringing a drug to market has historically been time-consuming and costly, with many candidates failing in the first phase of clinical trials. Growing evidence suggests that screening compounds early in the process using traditional models – like animals and cell cultures propagated in 2D – may be a contributing factor. Researchers are now navigating toward complex, human-relevant 3D systems like [organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids "organoids"), as they are believed to better represent how the body might respond to treatment. Experience our interactive infographic as it takes you deeper into why the industry is embracing this next generation of drug discovery and the innovations supporting scientists in their 3D biology journey. DRUG DISCOVERY PARADIGM   [Powered by Ceros](http://www3.ceros.com/poweredbyceros?utm_source=M-W-CerosPower&account=molecular-devices&experience=drug-discovery-paradigm) Close modal _[Access PDF to download](https://www.moleculardevices.com/en/assets/brochures/dd/img/3d-biology-drug-discovery-paradigm "Access PDF to download")_ ### What is the Drug Discovery Paradigm? 3D biology is the next-generation of drug discovery – a transition away from 2D cell cultures and animal models to more biologically relevant 3D cell models and human systems. Begin your journey from 2D to 3D biology within our latest blog as we show you what it takes to enter into this new drug discovery paradigm and how researchers worldwide are transitioning their compound screens away from 2D cell cultures and animal models to more complex, human-relevant 3D systems like organoids. - [The revolutionary promise of 3D biology](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#the-revolutionary-promise-of-3d-biology) - [The expensive and lengthy path of drug development](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#the-expensive-and-lengthy-path-of-drug-development) - [Limitations of conventional cell models](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#limitations-of-conventional-cell-models) - [Regulatory boost for organoids](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#regulatory-boost-for-organoids) - [Organoid technology](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#organoid-technology) - [3 key organoid use cases](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#key-organoid-use-cases) - [The challenges with organoids](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#the-challenges-with-organoids) - [Radical innovation required](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#radical-innovation-required) - [Organoid screening workflow](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#organoid-screening-workflow) - [Visit the Organoid Innovation Center](https://www.moleculardevices.com/lab-notes/3d-biology/3d-biology-paradigm-shift-for-next-gen-drug-discovery#visit-the-organoid-innovation-center) ### The revolutionary promise of 3D biology Human-relevant models provide early insights into drug interactions with human cells, detecting efficacy and [toxicity](https://www.moleculardevices.com/applications/toxicology "toxicity") concerns pre-clinically. The emerging field of 3D biology is revolutionizing drug screening and disease understanding, offering a unique advantage in predicting human responses to new treatments. 3D cell models like organoids have a unique makeup that offer a step-change in predicting human responses to novel treatments. Their increased physiological relevance leads to more accurate indications of a therapeutic’s efficacy in the pre-clinical phase. This allows for a weeding out of toxic, ineffective compounds to make space for those with healing power earlier in the drug discovery process. ![Molecular Devices 3D biology Experts](https://www.moleculardevices.com/lab-notes/3d-biology/media_186027fd918f3dfb2884210aac30354acc1d3bc9f.png?width=750&format=png&optimize=medium) We believe in the revolutionary promise of 3D biology, where organoids accurately predict real-world patient responses to novel treatments, offering: - Less animal testing - Safer clinical trials - And ultimately, radically improved human health In this piece, " [Expert Perspectives on 3D Biology](https://www.moleculardevices.com/lab-notes/3d-biology/expert-perspectives-on-3d-biology "Expert Perspectives on 3D Biology")," we’ve curated insights from our in-house experts on the role 3D biology plays in making next-gen drug discovery a reality. ### The expensive and lengthy path of drug development Despite billion-dollar investments, getting new drugs approved is still a lengthy and costly process, mainly due to high attrition rates. Traditional 2D cell cultures and animal models often fail to predict how drugs will perform in humans, leading to many potential drugs falling short during development. ![Drug Discovery Triel](https://www.moleculardevices.com/lab-notes/3d-biology/media_1c9499d862f02f6fd06b39ede9f3931ca99dba0c9.png?width=750&format=png&optimize=medium) A staggering 75% of research and development costs come from failures—funds spent on projects that initially seem promising but are later found ineffective, unsafe, or commercially unviable during human trials. It's clear that the drug development process needs a revolution, and organoids might be the key to achieving it. ### Limitations of conventional cell models Ethical consideration aside, traditional cell models in drug research – whether 2D cell lines, small animal models, or non-human primates – face translatability challenges that hinder their success in moving therapeutics from bench to bedside. ![conventional cell models](https://www.moleculardevices.com/lab-notes/3d-biology/media_17e13f1cb35d8e619855ab2a69093ba18bca34df8.png?width=750&format=png&optimize=medium) To address this, the Drug Discovery Paradigm leverages human-relevant model systems throughout drug discovery to improve clinical success rates and reduce dependency on animal models. Organoids are reducing the translatability gap by accurately predicting drug outcomes. Studies indicate that drugs failing on patient organoids in pre-clinical trials will likely fail in actual patients, while a high success rate will translate to effective patient outcomes. Despite their potential, growing organoids can be labor-intensive and time-consuming, affecting scalability. However, [automated cell culture](https://www.moleculardevices.com/products/3d-biology/cellxpress-ai-automated-cell-culture-system "automated cell culture") technologies and [assay-ready organoids](https://www.moleculardevices.com/products/3d-biology#3d-ready-organoids-and-organoid-expansion-service-1 "assay-ready organoids") are enhancing scalability to make their adoption more feasible. ### Regulatory boost for organoids ![FDA Modernization Act 2.0](https://www.moleculardevices.com/lab-notes/3d-biology/media_11cfa92078f210137f09c4c5918d81a781b02f4f3.png?width=750&format=png&optimize=medium) The FDA has demonstrated increased support for organoid and other complex in vitro model use in research. This was evidenced in 2022 when Congress passed the [FDA Modernization Act 2.0](https://www.moleculardevices.com/newsroom/in-the-news/what-transition-away-from-animal-testing-could-mean-for-drug-discovery "FDA Modernization Act 2.0"), allowing new drugs to be approved using methods other than animal testing for the first time. A shift set to make drug discovery more ethical and potentially more effective and efficient. ### Organoid technology These miniature versions of organs are revolutionizing medical research with their unique capabilities. Brain organoids, for instance, provide invaluable insights into human brain development and neurological diseases, making them ideal for testing pharmaceutical drugs and studying genetic mutations. Intestinal organoids, with their accurate replication of the intestinal epithelium, are crucial for understanding cell biology, regeneration, and disease phenotypes related to the gut. Tumoroids, derived from patient tumors, are game-changers in cancer research, enabling personalized medicine by allowing researchers to analyze tumor biomarkers and screen for the most effective anti-cancer drugs. Lung organoids replicate the complex structure and function of the airway, making them perfect for drug screening and disease modeling in respiratory conditions. ![Organoid technology](https://www.moleculardevices.com/lab-notes/3d-biology/media_1c0855d8a6ba520fb8b9a1a13d626cd9493b98835.png?width=750&format=png&optimize=medium) Collectively, these diverse organoid types are paving the way for groundbreaking advancements in medical research and therapeutic development. - [Brain Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/brain-organoids "Brain Organoids") - [Breast Cancer Tumoroids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/breast-cancer-organoids "Breast Cancer Tumoroids") - [Cardiac Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids#cardiac-organoids "Cardiac Organoids") - [Colorectal Cancer (CRC) Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/colorectal-cancer-crc-organoids "Colorectal Cancer (CRC) Organoids") - [Intestinal Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/intestinal-organoids "Intestinal Organoids") - [Patient-derived organoids (Tumoroids)](https://www.moleculardevices.com/applications/3d-cell-models/organoids/patient-derived-organoids "Patient-derived organoids (Tumoroids)") - [Pulmonary (Lung) Organoids](https://www.moleculardevices.com/applications/3d-cell-models/organoids/pulmonary-lung-organoids "Pulmonary (Lung) Organoids") ### 3 key organoid use cases Because organoids so closely resemble their internal organ counterparts, they have proven to be an accurate 3D cell model for studying human disease, screening drugs, and testing potential therapeutics. They’re also an especially useful tool for precision medicine. For example, patient derived organoids can be generated from individuals with specific ailments and used to better understand disease, develop drugs, and customize personalized therapies. ![organoid use cases](https://www.moleculardevices.com/lab-notes/3d-biology/media_1f8d6f1d4ce0aec47baffa0a642f2ce16cb0ed2fd.png?width=750&format=png&optimize=medium) **DISEASE MODELING** [Disease modeling](https://www.moleculardevices.com/applications/disease-modeling "Disease modeling") is a cornerstone of biomedical research, offering systems that mimic the behavior of diseases in controlled environments to uncover their underlying mechanisms and test potential therapies. While traditional models like 2D cell cultures and animals have limitations, organoids offer a reliable alternative by replicating human tissue complexity. This makes them ideal for studying diseases such as Alzheimer's, heart failure, and cancer. Organoids could also provide a more accessible solution for advancing rare disease research, potentially unearthing treatment for the [7,000 rare diseases affecting 400 million people](https://pmc.ncbi.nlm.nih.gov/articles/PMC8378213/ "7,000 rare diseases affecting 400 million people") globally, of which only [500 are actively studied](https://www.nih.gov/about-nih/what-we-do/nih-turning-discovery-into-health/promise-precision-medicine/rare-diseases "500 are actively studied"). **DRUG SCREENING** [High-throughput drug screening](https://www.moleculardevices.com/applications/drug-discovery-and-development "High-throughput drug screening") (HTS) revolutionized the 1990s by enabling rapid testing of multi-million-compound libraries using multi-well plates. The approach aimed to test every compound in an extensive library to find a few active ones, but the process was cumbersome and often failed to produce clinically relevant results. Today, the shift from 2D models to advanced 3D biological models like organoids – combined with automation and AI technology – is enhancing the precision and relevance of HTS, making it possible to pinpoint the most promising drug candidates faster. **PDOs** [Patient-derived organoids](https://www.moleculardevices.com/newsroom/in-the-news/patient-derived-organoids-in-disease-modelling "Patient-derived organoids") (PDOs), or tumoroids, are revolutionizing drug discovery and cancer research by offering human relevant models derived from individual patients' tumors. Generated from tumor biopsies or surgical samples, these 3D cultures maintain the molecular characteristics of the original tumors and exhibit cancer stem cell-like properties. This makes them invaluable for understanding patient-specific drug responses, analyzing tumor biomarkers, and screening for the most effective anti-cancer drugs. In challenging cancers like breast and colorectal cancer, PDOs have shown superior potential in precision medicine and high-throughput drug discovery applications, particularly when supported by automation, high-content imaging, and AI data analysis. ### The challenges with organoids Adopting 3D biology can present significant barriers, despite the promise of organoids offering greater predictability and more biologically relevant data than 2D cell models. However, understanding these challenges is the first step toward finding solutions to streamline processes and deliver safer, much-needed therapies to patients faster. ![challenges with organoids](https://www.moleculardevices.com/lab-notes/3d-biology/media_193b1b59d59a73c05476b1df401134f71365148e4.png?width=750&format=png&optimize=medium) ### Radical innovation required Embracing innovation in organoid technology is essential to revolutionizing drug discovery. By advancing protocols, leveraging automation, and incorporating the power of AI, we can improve accuracy, reduce costs, and accelerate drug discovery timelines. ![Radical innovation in organoid technology](https://www.moleculardevices.com/lab-notes/3d-biology/media_1baa2c1ac73da6f2b44f880dc1b9a90a7a959d7b5.png?width=750&format=png&optimize=medium) **Assay-ready Organoids** Through our [3D Ready™ Organoids and Organoid Expansion Service](https://www.moleculardevices.com/products/3d-biology#3d-ready-organoids-and-organoid-expansion-service-1 "3D Ready™ Organoids and Organoid Expansion Service"), we use proprietary bioreactor and bioprocess technology to produce high-quality, reliable PDOs at scale. This includes both assay-ready organoids from curated lines and the ability to expand proprietary organoid lines to meet specific research needs, providing consistent and high-quality human-relevant models for a variety of applications. **High-throughput, High-content Imaging** High-performance imagers play a crucial role in scientific research by providing the detailed, high-resolution images necessary for accurate data analysis and discovery. Our growing line of [ImageXpress® high-content imaging systems](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-imaging "ImageXpress® high-content imaging systems") offer impeccable image quality, speed, data richness, and analytical accuracy, so researchers can clearly visualize complex biological processes for greater insights. **Artificial Intelligence** [IN Carta® Image Analysis Software](https://www.moleculardevices.com/products/cellular-imaging-systems/high-content-analysis/in-carta-image-analysis-software "IN Carta® Image Analysis Software") is a powerful AI/ML software that revolutionizes image analysis and workflow efficiency for scientific research. Integrated into our automated cell culture system, it simplifies decision-making and enhances experiment accuracy by using images to ultimately inform consistent workflow automation, optimal cell culture feeding, and timely passaging events. The analysis capability also transforms complex images into easily interpretable results, inviting researchers to explore data, identify phenotypic changes, and uncover novel insights effortlessly without the need for deep data science expertise. **Automated Cell Culture** The [CellXpress.ai® Automated Cell Culture System](https://www.moleculardevices.com/products/3d-biology/cellxpress-ai-automated-cell-culture-system "CellXpress.ai® Automated Cell Culture System") alleviates repetitive tasks for organoid growth by integrating machine learning to manage complex feeding and passaging schedules. By combining advanced hardware and software technologies, this system automates and standardizes 2D and 3D cell culture processes, supporting scientists at every stage of organoid research and guiding them through workflows for iPSC, tumoroid, and adult stem cell-derived organoids. Its AI-powered software, efficient protocol development, and comprehensive event logging deliver reliable and reproducible results at scale. [https://share.vidyard.com/watch/xgTqMVm4uWCZ4sfkPxycyE](https://share.vidyard.com/watch/xgTqMVm4uWCZ4sfkPxycyE) ### Organoid screening workflow Implementing an effective, automated end-to-end workflow for advanced drug discovery depends on its ability to standardize and streamline the organoid development process, enhancing both throughput and accuracy. By integrating proven hardware and software solutions, an automated workflow can facilitate everything from 2D pre-culture of cells to high-content confocal imaging, and AI-enabled data analysis. ![Organoid screening workflow](https://www.moleculardevices.com/lab-notes/3d-biology/media_122ef524c0a955209c6ca369c11bc4ee484ac9ebd.png?width=750&format=png&optimize=medium) Our automated high-throughput screening solution offers protocols to manage cell plating, culture monitoring, media exchange, and compound treatment, covering both matrix-based and matrix-free organoid cultures. Comprehensive automation not only improves the precision of organoid assays but also allows for complex, quantitative analysis of cellular content and phenotypic changes, making it invaluable for drug screening and disease modeling. ### Visit the Organoid Innovation Center The [Organoid Innovation Center](https://www.moleculardevices.com/applications/organoid-innovation-center "Organoid Innovation Center") demonstrates a fully-integrated solution that addresses the challenges associated with every step in the sample prep-to-report pipeline for assays performed on complex 3D biological models. An end-to-end solution standardizes the organoid development process with cell culture, treatment, and incubation, through to imaging, analysis, and data processing, delivering consistent, unbiased, and biologically-relevant results at scale. ![Organoid Innovation Center](https://www.moleculardevices.com/lab-notes/3d-biology/media_1d5f98231375517529bdd95ea8960048bdc252457.png?width=750&format=png&optimize=medium) Has this inspired you to explore a transition from 2D to 3D biology? To find solutions for your organoid challenges? Or to scale the work you’re already doing? Our experts can help. Reach out to us today to get started. **[Inquire about 3D Biology](https://www.moleculardevices.com/quote-request?pid=3d-biology "Inquire about 3D Biology")** _[Explore 3D Biology Solutions](https://www.moleculardevices.com/products/3d-biology "Explore 3D Biology Solutions")_ #### Recent posts [![A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/3d-biology/media_135347bfb9e0627730d814fb2b96129223194220b.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) ### [A Brief History of Patch-Clamping — Part 1: Laying the Groundwork for Electrophysiology](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) Explore the origins of patch-clamp electrophysiology—foundational… [Create history with AXON](https://www.moleculardevices.com/lab-notes/axon-patch-clamp/history-of-patch-clamp-part-1) [![The 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Subscribe ![Close Video](https://www.moleculardevices.com/icons/close-video.svg?width=750&format=svg&optimize=medium) ## SpectraMax M Series Readers ![SpectraMax M Series Multi-Mode Microplate Readers](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_11e468ddf27f690fc4468853162827ecefd6da632.jpg?width=1200&format=jpg&optimize=medium) # SpectraMax M Series Multi-Mode Microplate Readers ![SpectraMax M Series Multi Mode Microplate Readers](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_18644ad9a37995fd877f2ba1f673732ea5953e457.png?width=750&format=png&optimize=medium) Configurable readers with triple-mode cuvette ports, validation tools, and compliance software **[Talk to an expert](https://www.moleculardevices.com/quote-request?request_type=Call&pid=spectramax-m-series-multi-mode-microplate-readers "Talk to an expert")** _[Download brochure](https://chat.moleculardevices.com/spectramax-mseries-multi-mode-microplate-readers "Download brochure")_ ![](https://www.moleculardevices.com/images/play_icon.png) - [Overview](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#overview) - [Applications](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) - [Specifications & Options](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#specifications-options) - [Resources](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#resources) - [Citations](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#citations) - [Order](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#order) - [Related Products & Services](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#related-products-services) ## Dual monochromators increase performance and assay flexibility The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance. ![Assay flexibility Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_158b710520270c41d863c0e0abf36f5f602f70880.png?width=750&format=png&optimize=medium) ### Assay flexibility The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. ![Accuracy Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_12070f39735e9ac43337aa427d1d95470163a502d.png?width=750&format=png&optimize=medium) ### Better absorbance accuracy Measure sample depth with no temperature dependency using the PathCheck Sensor technology. It automatically normalizes absorbance readings to 1 cm, eliminating the need for standard curves. ![Comprehensive Reader Validation Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1112196b0dbd2245fbc510713bff8a58a99a05e2a.png?width=750&format=png&optimize=medium) ### Increased throughput Integrate easily with our StakMax® Microplate Stacker for walk-away automation. The stacker can hold up to 50 plates and can be configured to include a barcode reader. ![](https://play.vidyard.com/37tardjo4nnG-x5fiYuWjw.jpg) SpectraMax M5e Multi-Mode Microplate Reader ## Features ![Expandable Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1124a9c8fc6f2d78b1ee36fff51fdaa77c8d64c5b.png?width=750&format=png&optimize=medium) ### Patented optimization The [patented](https://www.moleculardevices.com/patents "patented") AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. ![Protocols Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1734bd2f949b3086a61ee3a40747f27ae3a0f1a8a.png?width=750&format=png&optimize=medium) ### IQ/OQ/PM Services Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely. ![Validation Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_195fee8cd23affc8e112a74455c62f0bf23bc2a34.png?width=750&format=png&optimize=medium) ### Validation tools Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data. ![Compliance Software Icon](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_19933413a8d5a06fa4f43c1973eb3189a71f246e6.png?width=750&format=png&optimize=medium) ### Compliance software [SoftMax Pro GxP Software](https://www.moleculardevices.com/products/gxp-compliance-solutions/softmax-pro-gxp-software "SoftMax Pro GxP Software") extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records. ## Kickstart your compliance journey with our _“GxP Starter Kit”_ ![SpectraMax M Series with GxP Starter Kit](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_10a394a0104a2e15004d3cea7306e574bc1fd19ed.png?width=750&format=png&optimize=medium) For researchers working in GLP or GMP laboratories, achieve full [GxP compliance](https://www.moleculardevices.com/products/gxp-compliance-solutions "GxP compliance") with our turnkey solution: - **SpectraMax M-Series Microplate Reader** are modular and upgradeable with a wide range of high performance capabilities. - **SoftMax® Pro 7.2 GxP Software –** The latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. - **SoftMax® Pro Validation Package –** Provides the most comprehensive documentation and tools available to validate GxP administrator features, software operation, and analysis functions for microplate reader instrumentation. - **Software 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solution for microplate data… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/importance-of-softmax-pro-software-in-microplate-data-analysis) [![The Essentials of Parallel Line Analysis in Bioassays: A Comprehensive Guide to PLA Methods and Software Tools](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1af935e1c7f561c7f60fd7687cf59fded0a259129.jpeg?width=800&format=jpeg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/essentials-of-parallel-line-analysis-in-bioassays) Blog Jul 18, 2024 ### [The Essentials of Parallel Line Analysis in Bioassays: A Comprehensive Guide to PLA Methods and Software Tools](https://www.moleculardevices.com/lab-notes/microplate-readers/essentials-of-parallel-line-analysis-in-bioassays) Discover the importance of Parallel Line Analysis (PLA) in pharmaceutical… [Learn 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more](https://www.moleculardevices.com/en/assets/app-note/br/fujifilm-bacterial-endotoxin-assays-on-spectramax-readers) [![SoftMax Pro Software: User-Friendly Software in Microplate Data Analysis](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_168fb196940f8f4f7531c2185edee7bb23d233cbb.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/lab-notes/microplate-readers/importance-of-softmax-pro-software-in-microplate-data-analysis) Blog Oct 10, 2024 ### [SoftMax Pro Software: The Importance of User-Friendly Software in Microplate Data Analysis](https://www.moleculardevices.com/lab-notes/microplate-readers/importance-of-softmax-pro-software-in-microplate-data-analysis) Explore SoftMax Pro Software, the leading solution for microplate data… [Learn more](https://www.moleculardevices.com/lab-notes/microplate-readers/importance-of-softmax-pro-software-in-microplate-data-analysis) [![The Essentials of Parallel Line Analysis in 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Devices](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_104e9f191a6b839012e7b0cdc5a423d3445264619.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autosave-option-softmax-pro-gxp-7) Videos and Webinars Jul 10, 2025 ### [Using AutoSave Option in SoftMax Pro GxP Software 7](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autosave-option-softmax-pro-gxp-7) Learn how to use the AutoSave feature in SoftMax Pro GxP Software 7 to… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autosave-option-softmax-pro-gxp-7) [![AutoExport Option in SoftMax Pro GxP Software 7 | Molecular Devices](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1b6635a08e06dbed848a297c9fc3d8330dba11429.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autoexport-option-softmax-pro-gxp-7) Videos and Webinars Jul 10, 2025 ### [Using AutoExport Option in SoftMax Pro GxP Software 7](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autoexport-option-softmax-pro-gxp-7) Learn how to use the AutoExport feature in SoftMax Pro GxP Software 7 to… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/br/using-autoexport-option-softmax-pro-gxp-7) **[View all Resources](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#resources "View all Resources")** ## Featured Applications ![SNP genotyping a process for analyzing genetic differences among individuals](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_10a2068c158ebfe5009d63f5eeb006b05f430e756.jpeg?width=750&format=jpeg&optimize=medium) ### SNP genotyping Genotyping is a process for analyzing genetic differences among… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![TRF, TR-FRET & HTRF](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1e8c3c7d4ba89e8bf010c2dbb74a5acc19472b24f.jpeg?width=750&format=jpeg&optimize=medium) ### TRF, TR-FRET & HTRF Learn all about time-resolved fluorescence – TRF and TR-FRET including… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Absorbance](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_18f5f01d8e53a55e60e563e7ea4e6496b3d03270b.jpeg?width=750&format=jpeg&optimize=medium) ### Absorbance Learn all about absorbance detection – how it works, how it’s measured,… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Cell Health](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1ae4fee1cc81124f1c7b085a97f920673463c6f6e.jpeg?width=750&format=jpeg&optimize=medium) ### Cell Health Cell viability refers to the number of healthy cells in a population and… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Cell viability](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1e905c40a49d2f038a97d0bbff8dbc7bafdee5b00.jpeg?width=750&format=jpeg&optimize=medium) ### Cell viability Cell line development requires the discovery of single cell-derived clones… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Cellular Signaling](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_160ca8123aba6a6c2214856b22fdd5c598869525e.jpeg?width=750&format=jpeg&optimize=medium) ### Cellular Signaling Cellular signaling allows cells to respond to their environment as well as… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Drug Discovery & Development Process](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1fea24937c7c28125b621f35b26badeb34d6d9ddc.jpeg?width=750&format=jpeg&optimize=medium) ### Drug Discovery & Development For every drug that makes it to the finish line, another nine don’t… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![ELISA (Enzyme-linked immunosorbent assays)](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1b958e2b9394e69c23a38b69c3ee3abb3700e06de.jpeg?width=750&format=jpeg&optimize=medium) ### ELISA Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Fluorescence](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_159d95ecea3465d266fc4b17ba8b269687473802a.jpeg?width=750&format=jpeg&optimize=medium) ### Fluorescence Learn all about fluorescence detection – what it is, how it works, and the… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Fluorescence Polarization (FP)](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_182ab87664be83b32d28e5137a80fcf421f8e043d.jpeg?width=750&format=jpeg&optimize=medium) ### Fluorescence Polarization (FP) Fluorescence polarization (FP) is a technique that is widely used to… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Fluorescent Protein Detection](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_19ccc335884b0340cf44b5945265e7c1e00693ba6.jpeg?width=750&format=jpeg&optimize=medium) ### Fluorescent Protein Detection Fluorescent proteins have become enormously popular as tools for… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Immunoglobulin G (IgG) Quantification](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1e12098a62a649f8232cab732c30e91f7df135e00.jpeg?width=750&format=jpeg&optimize=medium) ### IgG Quantification In therapeutic protein engineering and cell line development, IgG… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Luminescence](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1459bb4a035d40fbd257311ac36e4dade691c8a9b.jpeg?width=750&format=jpeg&optimize=medium) ### Luminescence Learn about luminescence detection – what it is, how it works, and the… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Microbiology and Contaminant](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1243e6fa7f74d8af6d2969300e5ca2ded46eb014d.png?width=750&format=png&optimize=medium) ### Microbiology and Contaminant Microbes, including bacteria, have been estimated to make up about 15… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Nucleic Acid (DNA/RNA) Quantitation and Analysis](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_11039eb2957f59ce1ab0307c46a489fe010a4bc79.jpeg?width=750&format=jpeg&optimize=medium) ### Nucleic Acid (DNA/RNA) Quantitation and Analysis Nucleic acids are large biomolecules common to all known life forms.… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Protein Detection, Quantitation and Analysis](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1d20ed6efefb779ac67d867cca5259aa709798cef.jpeg?width=750&format=jpeg&optimize=medium) ### Protein Detection, Quantitation and Analysis Protein detection, quantitation, and analysis are central to the… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![SNP genotyping a process for analyzing genetic differences among individuals](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_10a2068c158ebfe5009d63f5eeb006b05f430e756.jpeg?width=750&format=jpeg&optimize=medium) ### SNP genotyping Genotyping is a process for analyzing genetic differences among… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![TRF, TR-FRET & HTRF](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1e8c3c7d4ba89e8bf010c2dbb74a5acc19472b24f.jpeg?width=750&format=jpeg&optimize=medium) ### TRF, TR-FRET & HTRF Learn all about time-resolved fluorescence – TRF and TR-FRET including… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Absorbance](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_18f5f01d8e53a55e60e563e7ea4e6496b3d03270b.jpeg?width=750&format=jpeg&optimize=medium) ### Absorbance Learn all about absorbance detection – how it works, how it’s measured,… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ![Cell Health](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1ae4fee1cc81124f1c7b085a97f920673463c6f6e.jpeg?width=750&format=jpeg&optimize=medium) ### Cell Health Cell viability refers to the number of healthy cells in a population and… [Read more](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers#applications) ## Customer Breakthrough ![American Heritage School uses SpectraMax M5e Reader to conduct Scientific Research](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_1f008c9b14e2eef4808c0ce5976e1ece6821e56bd.png?width=750&format=png&optimize=medium) SUCCESS STORY ### American Heritage School uses the SpectraMax M5e reader to conduct scientific research [Continue Reading](https://www.moleculardevices.com/en/assets/customer-breakthrough/br/american-heritage-school "Continue Reading") ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=spectramax-m-series-multi-mode-microplate-readers "Speak to a specialist")** ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Applications of SpectraMax M Series Multi-Mode Microplate Readers [Absorbance](https://www.moleculardevices.com/fragments/applications/absorbance "Absorbance") [Cell Health](https://www.moleculardevices.com/fragments/applications/cell-health "Cell Health") [Cell viability](https://www.moleculardevices.com/fragments/applications/cell-viability "Cell viability") [Cellular Signaling](https://www.moleculardevices.com/fragments/applications/cellular-signaling "Cellular Signaling") [Drug Discovery & Development](https://www.moleculardevices.com/fragments/applications/drug-discovery-and-development "Drug Discovery & Development") [ELISA](https://www.moleculardevices.com/fragments/applications/elisa "ELISA") [Fluorescence](https://www.moleculardevices.com/fragments/applications/fluorescence "Fluorescence") [Fluorescence Polarization (FP)](https://www.moleculardevices.com/fragments/applications/fluorescence-polarization-fp "Fluorescence Polarization (FP)") [Fluorescent Protein Detection](https://www.moleculardevices.com/fragments/applications/fluorescent-protein-detection "Fluorescent Protein Detection") [IgG Quantification](https://www.moleculardevices.com/fragments/applications/igg-quantification "IgG Quantification") [Luminescence](https://www.moleculardevices.com/fragments/applications/luminescence "Luminescence") [Microbiology and Contaminant](https://www.moleculardevices.com/fragments/applications/microbiology-and-contaminant "Microbiology and Contaminant") [Nucleic Acid (DNA/RNA) Quantitation and Analysis](https://www.moleculardevices.com/fragments/applications/nucleic-acid-dna-rna-quantitation-and-analysis "Nucleic Acid (DNA/RNA) Quantitation and Analysis") [Protein Detection, Quantitation and Analysis](https://www.moleculardevices.com/fragments/applications/protein-detection-quantitation-and-analysis "Protein Detection, Quantitation and Analysis") [SNP genotyping](https://www.moleculardevices.com/fragments/applications/snp-genotyping "SNP genotyping") [TRF, TR-FRET & HTRF](https://www.moleculardevices.com/fragments/applications/trf-tr-fret-htrf "TRF, TR-FRET & HTRF") [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers [Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers](https://www.moleculardevices.com/products/specifications/spectramax-m-series-readers.json "Specifications & Options of SpectraMax M Series Multi-Mode Microplate Readers") _\*Using lowest settings and speed read when available._ ### [Download Brochure](https://chat.moleculardevices.com/spectramax-mseries-multi-mode-microplate-readers "Download Brochure") ![download pdf](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/media_114e4e5ccf8efcad1115a9dc371921ff8e7dfae10.png?width=750&format=png&optimize=medium) [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Resources of SpectraMax M Series Multi-Mode Microplate Readers [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## SpectraMax® M Series Multi-Mode Microplate Readers **Product** **Part Number** SpectraMax M2 Multi-Mode Microplate Reader M2 SpectraMax M2E Multi-Mode Microplate Reader M2E SpectraMax M3 Multi-Mode Microplate Reader M3 SpectraMax M4 Multi-Mode Microplate Reader M4 SpectraMax M5 Multi-Mode Microplate Reader M5 SpectraMax M5E Multi-Mode Microplate Reader M5E SpectraTest ABS2 Absorbance Validation Plate 0200-6191 SpectraTest FL1 Fluorescence Validation Plate 0200-5060 SpectraTest LM1 Luminescence Validation Plate 0200-6186 SpectraDrop™ Micro-Volume Starter Kit 0200-6262 SpectraDrop™ Micro-Volume HTS Kit 0200-6267 StakMax Microplate Handling Stacker StakMax Reader Shelf 9000-0756 [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products & Services of SpectraMax M Series Multi-Mode Microplate Readers [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Synthetic Biology Innovations ![Synthetic Biology](https://www.moleculardevices.com/applications/media_1ad97a9fca97b6620136ebbcab10f380edb1479c0.jpg?width=1200&format=jpg&optimize=medium) # Synthetic Biology Re-engineer biology to solve some of the world’s most pressing problems. **[Download Synthetic Biology eBook](https://www.moleculardevices.com/en/assets/ebook/bpd/synthetic-biology-for-sustainable-world "Download Synthetic Biology eBook")** - [Overview](https://www.moleculardevices.com/applications/synthetic-biology#overview) - [Resources](https://www.moleculardevices.com/applications/synthetic-biology#resources) - [Related Products & Services](https://www.moleculardevices.com/applications/synthetic-biology#related-products-services) ## What is synthetic biology? Synthetic biology is a broad term referring to the manipulation of metabolic pathways to coax microbes to manufacture proteins in a highly predictable manner through the use of engineering principles, like the Design-Build-Test-Learn Approach (DBTL). Sometimes individual genes are modified and sometimes entire genomes are edited for desired characteristics. Synthetic biology is an interdisciplinary field that combines principles from biology, engineering, and computer science to design and construct biological parts, devices, and systems with novel functions or capabilities. It has the potential to impact academic and industrial applications, including the creation of novel therapeutics and vaccines, plant science, and biofuels. The focus is often on generating, characterizing, and isolating parts of natural biological systems before using them as components of an engineered biological pathway. ## Benefits of synthetic biology The concept of a bio-based economy is central to the vision of a sustainable future powered by synthetic biology. This economy relies on renewable biological resources to produce food, materials, and energy, reducing dependence on fossil fuels and minimizing environmental impact. By transforming how we produce and consume resources, synthetic biology has the potential to create more sustainable and resilient economies. Synthetic biology represents a transformative approach to solving some of the world's most pressing challenges. By leveraging the principles of genetic engineering and biology, this field offers innovative solutions that can drive progress in healthcare, food production, and environmental sustainability. ![Fermentalg® uses QPix® 420 colony picker to leverage microalgae metabolism to produce natural food dyes](https://www.moleculardevices.com/applications/media_101b37055dc41bda24e6b424e39b38773f9d31d17.jpg?width=750&format=jpg&optimize=medium) _Customer Breakthrough: [Fermentalg® uses QPix® 420 colony picker to leverage microalgae metabolism to produce natural food dyes](https://www.moleculardevices.com/en/assets/customer-breakthrough/bpd/fermentalg "Fermentalg® uses QPix® 420 colony picker to leverage microalgae metabolism to produce natural food dyes")_ ## What is synthetic biology used for? The essence of synthetic biology lies in its ability to engineer life at its most fundamental level, allowing us to reimagine and recreate biological systems with unprecedented precision and purpose. From the rapid development of vaccines that can halt pandemics to the creation of cultured meat that reduces the environmental toll of traditional agriculture, synthetic biology is revolutionizing how we approach problems and develop solutions. ![CRISPR-Cas9 in disease research and treatment](https://www.moleculardevices.com/applications/media_182d8a14e9bfbe7a8ddcb7eea89e0721482c90906.jpg?width=750&format=jpg&optimize=medium) _For a deeper dive into the latest advancements and applications of CRISPR-Cas9 in disease research and treatment, [read the blog](https://www.moleculardevices.com/lab-notes/clone-screening/crispr-cas9-gene-editing "read the blog") and tune into the recent episode of the Drug Target Review podcast from Molecular Devices, featuring Dr. Pietro De Angeli from the Institute for Ophthalmic Research at the University Hospital Tübingen, and Dr. Maarten from the Princess Maxima Center._ ### **Healthcare:** Synthetic biology is revolutionizing healthcare by enabling the development of new therapies and vaccines. For instance, engineered bacteria and viruses can be used to deliver therapeutic genes to patients, while synthetic biology techniques are being employed to produce complex biologic drugs more efficiently. In addition to vaccines, synthetic biology is also being used to develop new therapies for genetic disorders. For example, [CRISPR-Cas9 technology](https://www.moleculardevices.com/applications/gene-editing-with-crispr-engineering "CRISPR-Cas9 technology") has shown promise in correcting genetic mutations that cause diseases such as sickle cell anemia and cystic fibrosis. By precisely editing the DNA of affected cells, researchers can potentially cure these diseases at their genetic root. This not only improves the quality of life for individuals affected by these conditions but also reduces the long-term healthcare costs associated with managing chronic diseases. ### **Sustainable Food Production:** By engineering microorganisms to produce food ingredients, synthetic biology can help address food scarcity and reduce the environmental impact of traditional agriculture. [Cultured meat](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/cell-cultured-meat "Cultured meat"), produced by growing animal cells in a lab, is one promising application that could significantly decrease the need for livestock farming and its associated environmental burdens. By growing animal cells in a lab environment, scientists can produce meat without the need for raising and slaughtering livestock. This approach significantly reduces greenhouse gas emissions, land use, and water consumption associated with conventional meat production. In addition to cultured meat, synthetic biology is being used to engineer microorganisms to produce food ingredients such as proteins, flavors, and vitamins. These bio-based ingredients can be produced more sustainably and with fewer resources than traditional agricultural methods. For example, [precision fermentation](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/precision-fermentation "precision fermentation") can produce proteins and other nutrients at scale, contributing to a more resilient and sustainable food system. **[Download the Cultured Meat eBook](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table "Download the Cultured Meat eBook")** ![Watch the on-demand webinar, From Lab to Table: Advancing Global Food Sustainability](https://www.moleculardevices.com/applications/media_13f8436143feec15c29f105c64bc6f886a56fa52b.png?width=750&format=png&optimize=medium) _Watch the on-demand webinar, [From Lab to Table: Advancing Global Food Sustainability](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/advancing-global-food-sustainability-with-molecular-devices "From Lab to Table: Advancing Global Food Sustainability"), to learn more about sustainable food production._ ### **Environmental Management:** Synthetic biology can also contribute to environmental sustainability through the development of bio-based materials and the bioremediation of pollutants. Engineered microorganisms can be designed to break down environmental toxins or produce biodegradable plastics, offering greener alternatives to conventional industrial processes. ## Synthetic Biology Automation ![Synthetic Biology Automation](https://www.moleculardevices.com/applications/media_1a95509e16595dec493ed1692a5e68e07bb907334.png?width=750&format=png&optimize=medium) ### THE DESIGN-BUILD-TEST-LEARN (DBTL) APPROACH One of the foundational concepts in synthetic biology is the idea of "biobricks," standardized DNA sequences that can be assembled in various combinations to build genetic constructs. Following the approach of the DBTL cycle (design-build-test-learn), synthetic biology allows for the systematic and predictable engineering of biological systems, similar to how electronic components are used to construct complex circuits **[Find out more](https://www.moleculardevices.com/applications/synthetic-biology/dbtl-approach "Find out more")** ![Automating Synthetic Biology Workflow](https://www.moleculardevices.com/applications/media_11e0fc263f70cbe309a5825052ab351cf314c9922.png?width=750&format=png&optimize=medium) ### AUTOMATING YOUR SYNTHETIC BIOLOGY WORKFLOW High-throughput synthetic biology applications benefit greatly from the increased productivity of a robot-controlled microbial colony picker such as our [QPix Microbial Colony Picker](https://www.moleculardevices.com/products/clone-screening/microbial-screening/qpix-400-series-microbial-colony-pickers "QPix Microbial Colony Picker"), which picks up to 30,000 colonies per day with automatic pick run data tracking and database management. It can be integrated into an end-to-end molecular workflow, allowing users higher throughput and more walkaway time. **[View workflow](https://www.moleculardevices.com/applications/synthetic-biology/automating-synthetic-biology-workflow "View workflow")** ## Synthetic Biology Applications and Resources Synthetic biology enables the design and construction of bespoke biological systems with desired functionalities. - [Cell cultured meat for scalable production](https://www.moleculardevices.com/applications/synthetic-biology#cell-cultured-meat-for-scalable-production) - [Cellular agriculture and sustainable food systems](https://www.moleculardevices.com/applications/synthetic-biology#cellular-agriculture-and-sustainable-food-systems) - [Plasmid engineering in synthetic biology](https://www.moleculardevices.com/applications/synthetic-biology#plasmid-engineering-in-synthetic-biology) - [Precision fermentation for next-gen biomanufacturing](https://www.moleculardevices.com/applications/synthetic-biology#precision-fermentation-for-next-gen-biomanufacturing) - [Protein Engineering](https://www.moleculardevices.com/applications/synthetic-biology#protein-engineering) - [The use of synthetic biology in CRISPR technologies](https://www.moleculardevices.com/applications/synthetic-biology#the-use-of-synthetic-biology-in-crispr-technologies) ### Cell cultured meat for scalable production ![Cell Cultured Meat](https://www.moleculardevices.com/applications/media_1e2c41fc506bea18e6474a8d9941ee59ad4d54407.jpeg?width=750&format=jpeg&optimize=medium) Cell cultured meat is a product of cellular agriculture that uses biopsied animal cells to grow sustainable, humane meat as an alternative to the traditional farming industry. Because cell cultured meat is made with mammalian cells, many of the processes in developing and scaling a cell cultured meat product will mirror those in in biologics production, specifically cell line development. [Learn More](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/cell-cultured-meats) ### Cellular agriculture and sustainable food systems ![Cellular agriculture is the process of utilizing synthetic biology](https://www.moleculardevices.com/applications/media_18342110899689af1dbbf788d223eac3b5af51bec.jpeg?width=750&format=jpeg&optimize=medium) Cellular agriculture is the process of utilizing synthetic biology, cell line development, and protein engineering techniques to produce products that are traditionally harvested via farming, most notably proteins and fats for food products. [Learn More](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture) ### Plasmid engineering in synthetic biology ![Plasmid Production](https://www.moleculardevices.com/applications/media_18fed21ccd61a195049cdadf20f73fa03e0cb1fc9.jpeg?width=750&format=jpeg&optimize=medium) Plasmids are small, circular DNA molecules that operate independently from chromosomal DNA. Central to molecular biology, they enable the study, manipulation, and production of genes, playing a vital role in cloning, gene therapies, and vaccine development. [Learn more about plasmid production](https://www.moleculardevices.com/applications/plasmid) ### Precision fermentation for next-gen biomanufacturing ![Microbial Precision Fermentation](https://www.moleculardevices.com/applications/media_1707368c8a717a45ae137600caed9f0d96a720db0.jpeg?width=750&format=jpeg&optimize=medium) Cellular agriculture allows us to produce genuine animal proteins through microbial precision fermentation. Using synthetic biology techniques like strain engineering, scientists can engineer microbial cells as cellular factories for various organic molecules, most commonly proteins. In the food supply chain, precision fermentation is used to create everything from plant-based proteins from yeast cells to dairy- and animal-free whey protein from fungal strains. [Learn More](https://www.moleculardevices.com/applications/food-and-beverage/cellular-agriculture/precision-fermentation) ### Protein Engineering ![Protein Engineering](https://www.moleculardevices.com/applications/media_15cfbb317633b8ab8564a8220460408c5eeb02b71.jpeg?width=750&format=jpeg&optimize=medium) Protein engineering is a vital process in synthetic biology that enables the design and modification of proteins to enhance or introduce new functions. It plays a crucial role in accelerating advancements in fields like drug discovery, biotechnology, and industrial applications. By engineering proteins with specific traits, scientists can develop more efficient enzymes, therapeutic antibodies, and novel biomolecules. ### The use of synthetic biology in CRISPR technologies ![Gene editing is a genetic manipulation in living organism’s genomic DNA](https://www.moleculardevices.com/applications/media_12626e552e52a53f41d976487a00d0972343b32de.jpeg?width=750&format=jpeg&optimize=medium) Gene editing is a genetic manipulation in which a living organism’s genomic DNA is deleted, inserted, replaced, or modified. Gene editing is a site-specific targeting to create breaks in DNA through various techniques and does not always involve repair mechanisms. It consists of two techniques – inactivation and correction. Learn More about [Gene Editing](https://www.moleculardevices.com/applications/gene-editing-with-crispr-engineering) ## Latest Resources [![From Lab To Table](https://www.moleculardevices.com/sites/default/files/2024/from-lab-to-table.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) eBook Aug 01, 2024 ### [From Lab To Table](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) This eBook delves into one of the most groundbreaking advancements in food… [Learn more](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) [![A greener lab with QPix](https://www.moleculardevices.com/sites/default/files/2024/greener-lab-with-qpix.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) Infographic Jun 25, 2024 ### [A greener lab with QPix](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) Discover how QPix ensures lab sterility, reduces environmental impact, and… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) [![Automating Microbial Plating & Streaking with QPix FLEX System](https://www.moleculardevices.com/applications/media_119688ae63e427ef4ba1be9e310a9a8776cde6ce9.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) Application Note Jul 31, 2025 ### [Automating Microbial Plating and Streaking: Advancing Research with the QPix FLEX System](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) This study demonstrates the precision, reliability, and efficiency of the… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) [![The QPix FLEX Microbial Colony Picker ensures colony picking accuracy and efficiency in a contamination-free environment, preventing microbial cross-transfer](https://www.moleculardevices.com/applications/media_1d326d836512ecccfb7cd6db12d9126eb5c9e1de0.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) Application Note Jul 23, 2025 ### [The QPix FLEX Microbial Colony Picker ensures colony picking accuracy and efficiency in a contamination-free environment, preventing microbial cross-transfer](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) We show how the QPix FLEX Picker ensures colony picking accuracy and… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) [![Sustainable CRISPR-Based Genome Editing Therapies](https://www.moleculardevices.com/applications/media_1cb3c9895772872abbecffdc8e1130a4edad4c1e0.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/sustainable-crispr-genome-editing-therapies) Videos and Webinars Jun 04, 2025 ### [Establishing a Sustainable Platform for CRISPR-Based Genome Editing Therapies](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/sustainable-crispr-genome-editing-therapies) Watch how a CRISPR-based genome editing therapies platform accelerates… [Learn more](https://www.moleculardevices.com/en/assets/tutorials-videos/bpd/sustainable-crispr-genome-editing-therapies) [![QPix FLEX: Automated microbial plating, picking, and liquid handling for streamlined screening and hit selection](https://www.moleculardevices.com/sites/default/files/2025/qpix-flex-automated-microbial-plating-screening-hit-selection.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-flex-automated-microbial-plating-screening-hit-selection.pdf) Scientific Poster May 15, 2025 ### [QPix FLEX: Automated microbial plating, picking, and liquid handling for streamlined screening and hit selection](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-flex-automated-microbial-plating-screening-hit-selection.pdf) Explore how automated microbial colony picking with QPix FLEX improves… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-flex-automated-microbial-plating-screening-hit-selection.pdf) [![Engineering the Future: Synthetic Biology for a Sustainable World](https://www.moleculardevices.com/sites/default/files/2024/synthetic-biology-for-sustainable-world.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/bpd/synthetic-biology-for-sustainable-world) eBook Sep 18, 2024 ### [Engineering the Future: Synthetic Biology for a Sustainable World](https://www.moleculardevices.com/en/assets/ebook/bpd/synthetic-biology-for-sustainable-world) The ebook Engineering the Future: Synthetic Biology for a Sustainable… [Learn more](https://www.moleculardevices.com/en/assets/ebook/bpd/synthetic-biology-for-sustainable-world) [![CRISPR CAS 9 GENE Editing: The Art of DNA Design](https://www.moleculardevices.com/sites/default/files/2024/crispr-cas-9-gene-editing.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/bpd/crispr-cas-9-gene-editing) eBook Aug 22, 2024 ### [CRISPR CAS 9 GENE Editing: The Art of DNA Design](https://www.moleculardevices.com/en/assets/ebook/bpd/crispr-cas-9-gene-editing) This eBook will guide you through the complexities and possibilities of… [Learn more](https://www.moleculardevices.com/en/assets/ebook/bpd/crispr-cas-9-gene-editing) [![QPix Microbial Colony Pickers ensure sterility by preventing the risk of cross-contamination](https://www.moleculardevices.com/sites/default/files/2024/qpix-microbial-colony-pickers-ensure-sterility-by-preventing-risk-of-cross-contamination.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-microbial-colony-pickers-ensure-sterility-by-preventing-risk-of-cross-contamination.pdf) Scientific Poster Aug 20, 2024 ### [QPix Microbial Colony Pickers ensure sterility by preventing the risk of cross-contamination](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-microbial-colony-pickers-ensure-sterility-by-preventing-risk-of-cross-contamination.pdf) This study aims to demonstrate sterility during automated colony picking… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/scientific-posters/bpd/qpix-microbial-colony-pickers-ensure-sterility-by-preventing-risk-of-cross-contamination.pdf) [![From Lab To Table](https://www.moleculardevices.com/sites/default/files/2024/from-lab-to-table.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) eBook Aug 01, 2024 ### [From Lab To Table](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) This eBook delves into one of the most groundbreaking advancements in food… [Learn more](https://www.moleculardevices.com/en/assets/ebook/bpd/from-lab-to-table) [![A greener lab with QPix](https://www.moleculardevices.com/sites/default/files/2024/greener-lab-with-qpix.jpg?width=800&format=jpg&optimize=medium)](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) Infographic Jun 25, 2024 ### [A greener lab with QPix](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) Discover how QPix ensures lab sterility, reduces environmental impact, and… [Learn more](https://www.moleculardevices.com/sites/default/files/en/assets/infographics/bpd/greener-lab-with-qpix.pdf) [![Automating Microbial Plating & Streaking with QPix FLEX System](https://www.moleculardevices.com/applications/media_119688ae63e427ef4ba1be9e310a9a8776cde6ce9.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) Application Note Jul 31, 2025 ### [Automating Microbial Plating and Streaking: Advancing Research with the QPix FLEX System](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) This study demonstrates the precision, reliability, and efficiency of the… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/automated-microbial-plating-streaking-qpix-flex) [![The QPix FLEX Microbial Colony Picker ensures colony picking accuracy and efficiency in a contamination-free environment, preventing microbial cross-transfer](https://www.moleculardevices.com/applications/media_1d326d836512ecccfb7cd6db12d9126eb5c9e1de0.png?width=800&format=png&optimize=medium)](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) Application Note Jul 23, 2025 ### [The QPix FLEX Microbial Colony Picker ensures colony picking accuracy and efficiency in a contamination-free environment, preventing microbial cross-transfer](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) We show how the QPix FLEX Picker ensures colony picking accuracy and… [Learn more](https://www.moleculardevices.com/en/assets/app-note/bpd/qpix-flex-ensures-colony-picking-accuracy-efficiency) **[View all Resources](https://www.moleculardevices.com/applications/synthetic-biology#resources "View all Resources")** ## How can we help advance your next big discovery? Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today? I’d like to… **[Speak to a specialist](https://www.moleculardevices.com/quote-request?request_type=Call&pid=synthetic-biology "Speak to a specialist")** ## Resources for Synthetic Biology [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## Related Products And Services [How can we help advance your next big discovery?](https://www.moleculardevices.com/fragments/next-big-discovery "How can we help advance your next big discovery?") ## AI in Drug Screening # Revolutionizing High-Throughput Drug Screening With AI and Organoids Dec 11, 2023 ![Revolutionizing High-Throughput Drug Screening With AI and Organoids](https://www.moleculardevices.com/newsroom/in-the-news/media_1a056cd78757c5a72cab6d0122efc838044749d0f.jpg?width=750&format=jpg&optimize=medium) In this Technology Networks article, Molecular Devices’ Automation and Customization Commercial Manager Daniel DiSepio, PhD, discusses the evolution of high-throughput drug screening (HTS) on organoids in drug discovery, highlighting the shift from traditional methods to a more intelligent, computational approach facilitated by artificial intelligence (AI). [Go to article](https://www.technologynetworks.com/drug-discovery/articles/revolutionizing-high-throughput-drug-screening-with-ai-and-organoids-381697 "Go to article") Share this news ![wave](https://www.moleculardevices.com/images/wave-footer-bg-top.png?width=900&format=png&optimize=medium) ## Live Cell Imaging System ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/21d99e9a402a40622c864f3a15b6d7ece2117a0e308f244c865c1b53ef978110.jpg) Perform extended time-lapse acquisitions on living samples while maintaining optimal conditions # Enable live cell imaging and real-time environmental control Environmental control mimics the cell environment and enables you to run multi-day studies,time-lapse,and live cell assays.With CellReporterXpress? software, the ImageXpress? Pico Automated Cel Imaging System provides visibility for environmental control during acquisition to ensure that the system is running at peak performance during your assay. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/e0ae9ec4bf3f1f6be96a689b01d5a8ec680f9c2fbd54cdfaf2d6da73492a12d5.jpg) Reproduceand maintain environmental conditions ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/df924be927d605676210ff495dfc437a9abab7ac137054a36580b92d58d400bc.jpg) Timecourse for cell proliferation Time (24 hours) Monitor and measure changes in cell proliferation and cell phenotype over time with reliable timelapse imaging andon-the-flyimageanalysis. # Key features · Enable live cell imaging and 3D acquisition in an easy-to-use system to better identify phenotypic changes · Generate sharp images with a greater depth of field · Minimize evaporation with humidity controls · Maintain healthy cell growth environment with user-defined ${ \\mathsf { C O } } \_ { 2 }$ settings · Store the results of control parametrics with data for review even after the experiment ends · Study the effects of hypoxic conditions over time · Precise temperature regulation to mitigate thermal Z-drift throughout the entirety of the time-lapse acquisition · Ensure optimal environmental conditions at the plate level without the need to flood the entire system The external humidity chamber integratesvia the rearof the ImageXpress Pico system to maintain a small surface area for small lab spaces. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/93e9f36ed307ddf7628e3c2b56f603ba0010da04c843b04d05b72b44fa18694c.jpg) Microplates are placed in anenvironmental control cassette which introduces and maintains gas and humidity while reducing gas usage as compared to flooding the entire system. Cassettes can also be sterilized through UV. # Environmental control settings Setings can be configured through CellReporterXpress software and monitored during acquisition ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/4573b7c01f7d07b133d45653f40ba99340d8512e5c5aadcf2262d9b804638bb6.jpg) # Environmental control specifications | | | | --- | --- | | Temperature control | Ambient +8C to 40°C | | Temperature control homogeneity | 37C± 0.5°C at 23C ambient | | Gas control | O2 control,1-15% and ambient, CO2 control, ambient to 15% | | Humidity control | Active humidity control. Sample compartment controlled to 85% nominal humidity. | # For more information, please visit moleculardevices.com/pico The ImageXpress Pico system features optics by Leica Microsystems. | | | | | | --- | --- | --- | --- | | Contact Us | Regional Offices | | | | Phone: +1.800.635.5577 | USA and Canada +1.800.635.5577 | | Taiwan/Hong Kong +886.2.2656.7585 | | Web: www.moleculardevices.com | United Kingdom +44.118.944.8000 | Japan | +81.3.6362.9109 | | Email:info@moldev.com | Europe\* | 00800.665.32860 | South Korea +82.2.3471.9531 | | Check our website fora current | China | +86.4008203586 India | +91.73.8661.1198 | | listing of worldwide distributors. | \*Austria,Belgium,enmark,inand,France,Germanyreland,etherlands,pin,wedenndi | ## AccuClear Nano Assay Kit # SpectraMax® Quant™ # AccuClear™ Nano dsDNA Assay Kit # Quick Start Protocol for a 96-Well Microplate 1. Warm assay components to room temperature. 2. Prepare the working solution by diluting the AccuClear Nano dye (1:100) in 1X AccuClear Nano buffer and mix. 3. Pipette 200 µL of the working solution into each microplate well. 4. Pipette $1 0 ~ \\mu \\iota$ of the SpectraMax Quant AccuClear Nano dsDNA standards into the microplate wells and mix. 5. Pipette $1 0 ~ \\mu \\iota$ of each unknown DNA sample into separate microplate wells and mix. 6. Incubate the microplate at room temperature for 5 to 10 minutes in the dark. 7. Measure fluorescence in a fluorescence microplate reader $E X = 4 6 8$ nm, $E M = 5 0 7$ nm). Table 1-1: Preparation of dsDNA Standards | | | | | | --- | --- | --- | --- | | Std. | Final Conc. | Std. Volume | 1X AccuClear Nano Buffer Volume | | A | 25 ng/μL | 100 μL of 25 ng/μL AccuClear Nano Standard | None | | B | 10 ng/μL | 40 μL of 25 ng/μLAccuClear Nano Standard | 60 μL | | C | 3 ng/μL | 12 μL of 25 ng/μL AccuClear Nano Standard | 88 μL | | D | 1 ng/μL | 10 μL of 10 ng/μL of B | 90 μL | | E | 0.3 ng/μL | 10 μLof 3 ng/μL of C | 90 μL | | F | 0.1 ng/μL | 10 μLof 1 ng/μL of D | 90 μL | | G | 0.03 ng/μL | 10 μL of 0.3 ng/μL of E | 90 μL | | H | 0.01 ng/μL | 10 μL of 0.1 ng/μL of F | 90 μL | | | 0.003 ng/μL | 10 μL of 0.03 ng/μL of G | 90 μL | | J | 0 ng/μL | None | 100 μL | Complete instructions for using the SpectraMax Quant AccuClear Nano dsDNA Assay Kit can be downloaded from the Knowledge Base on the Molecular Devices support web site: [www.moleculardevices.com/support](http://www.moleculardevices.com/support) # Contact Us Phone: +1-800-635-5577 Web: Email: Visit our website for a current listing of worldwide distributors. ## AccuBlue Pico Assay Kit # SpectraMax® Quant™ # AccuBlue™ Pico dsDNA Assay Kit # Quick Start Protocol for a 96-Well Microplate 1. Warm assay components to room temperature. 2. Prepare the working solution by diluting the AccuBlue Pico dye (1:400) and AccuBlue Pico Enhancer (1:100) in 1X AccuBlue Pico buffer and mix. 3. Pipette $2 0 0 ~ \\mu \\iota$ of the working solution into each microplate well. 4. Pipette $1 0 \\mu \\iota$ of the SpectraMax Quant AccuBlue Pico dsDNA standards into the microplate wells and mix. 5. Pipette $1 0 \\mu \\iota$ of each unknown DNA sample into separate microplate wells and mix. 6. Incubate the microplate at room temperature for 5 to 10 minutes in the dark. 7. Measure fluorescence in a fluorescence microplate reader $E X = 4 6 8$ nm, $E M = 5 0 7$ nm). Table 1-1: Preparation of dsDNA Standards | | | | | | --- | --- | --- | --- | | Standard | Final Concentration | | Standard Volume 1X AccuBlue Pico Buffer Volume | | A | 300 pg/μL | AccuBlue Pico Standard | None | | B | 50 pg/μL | 10μLofA | 50 μL | | C | 10 pg/μL | 12 μLofB | 48 μL | | D | 2 pg/μL | 12 μL of C | 48 μL | | E | 0.5 pg/μL | 15 μL of D | 45 μL | | F | 0 pg/μL | None | 60 μL | Complete instructions for using the SpectraMax Quant AccuBlue Pico dsDNA Assay Kit can be downloaded from the Knowledge Base on the Molecular Devices support web site: [www.moleculardevices.com/support](http://www.moleculardevices.com/support) Click Knowledge Base, and then click Manuals and search for SpectraMax Quant AccuBlue Pico dsDNA. # Contact Us Phone: +1-800-635-5577 Web: Email: ## ScanLater Western Blot Assay ScanLater™WesternBlotAssayKit AssayKitExplorerKitBulkKit Eu-LabeledGoatAnti-MouseScanLater™WesternBlotAssayKitR8201R8205 Eu-LabeledGoatAnti-RabbitScanLater™WesternBlotAssayKitR8202R8204 Eu-LabeledDonkeyAnti-GoatScanLater™WesternBlotAssayKitR8224R8227 Eu-LabeledDonkeyAnti-RatScanLater™WesternBlotAssayKitR8225R8228 Eu-LabeledStreptavidinScanLater™WesternBlotAssayKitR8200R8203 Table1-1:AvailableKits QuickStartProtocol 1\. Dilutethebuffersolutionsandreconstitutetheantibodyorstreptavidinprovidedintheassaykit. 2\. Transferthegeltotheblottingmembraneasrequiredbyyourapplication. 3\. Soakthemembranein1Xwashingbufferforaminimumof1 minute. 4\. Blockthemembranein1Xblockingbufferfor1 hour. 5\. Addyourprimaryantibodydirectlyintotheblockingbufferatthecorrectdilutionforyourapplication andincubatefrom90 minutesto4 hours. 6\. Washthemembrane3timesin1Xwashbufferfor5 minuteseachtime. 7\. Dilutethepreviouslyreconstitutedantibodyorstreptavidinin1Xblockingbuffer. FortheExplorerKit,therecommendeddilutionis1:500. FortheBulkKit,therecommendeddilutionis1:5000. 8\. Addthedilutedantibodyorstreptavidintothemembraneandincubatefor60 minutes. 9\. Washthemembrane3timesin1Xwashbufferfor5 minuteseachtime. 10\. Rinsethemembranewithwaterfor15 secondsandletitdrybeforescanning.Themembranecanbe scannedwetrightafterthewashstep,butadrymembranehasahighersignalcomparedtoits background. 11\. ScanthemembraneinaSpectraMax ParadigmorSpectraMax i3Instrument. DownloadInstructions CompleteinstructionsforusingtheScanLater WesternBlotAssayKitcanbedownloadedfromthe KnowledgeBaseontheMolecularDevicessupportwebsite:www.moleculardevices.com/support.html ClickKnowledgeBase,andthenclickManualsandsearchforScanLaterorWesternBlot. www.moleculardevices.com R3613D November2014 ScanLaterWesternBlotAssayKit 2R3613D KitComponents ItemExplorerKitBulkKit ScanLater10XWashingBuffer100 mL,1 bottle 300 mL,3 bottlesat100 mLeach ScanLater5XBlockingBuffer40 mL,1 bottle 120 mL,1 bottle ScanLaterEu-LabeledAntibodyorStreptavidin20 μg,1 vial 60 μg,1 vial Table1-2:ComponentsoftheScanLaterWesternBlotAssayKits Beforeusingthecomponentsoftheassaykit,dilutethebuffersolutionsandreconstituteandaliquottheEu- labeledantibodyorstreptavidin. IfyouareusingtheExplorerKit,donotaliquotorfreezethereconstitutedantibodyorstreptavidin.Protect thesolutionfromlightandstoreitat4°Cforuptoone(1)week. TheExplorerKitandtheBulkKitdifferintheirdilutionrequirements. ItemExplorerKitBulkKit ReconstituteEu-labeledantibodyorstreptavidin 200 μLof1x blockingbuffer 60 μLofreagent-gradewater Recommendeddilutionofreconstitutedantibody orstreptavidinfortheassay 1:5001:5000 Table1-3:DilutionDifferencesintheScanLaterWesternBlotAssayKits ItemVolumePart Number ScanLater10X WashingBuffer100 mL bottleR8206 ScanLater5X BlockingBuffer120 mL bottleR8207 ScanLaterEu-LabeledGoatAnti-MouseIgG60 μg vialR8208 ScanLaterEu-LabeledGoatAnti-RabbitIgG60 μg vialR8209 ScanLaterEu-LabeledDonkeyAnti-GoatIgG60 μg vialR8226 ScanLaterEu-LabeledDonkeyAnti-RatIgG60 μg vialR8229 ScanLaterEu-LabeledStreptavidin60 μg vialR8212 Table1-4:SeparatelyOrderableComponents Forresearchuseonly.Notforuseindiagnosticprocedures. ThetrademarksmentionedhereinarethepropertyofMolecularDevices,LLCortheirrespectiveowners.Thesetrademarksmaynot beusedinanytypeofpromotionoradvertisingwithoutthepriorwrittenpermissionofMolecularDevices,LLC. Patents: http://www.moleculardevices.com/productpatents ProductmanufacturedbyMolecularDevices,LLC. 1311OrleansDrive,Sunnyvale,California,UnitedStatesofAmerica94089. MolecularDevices,LLCisISO9001registered. ©2014MolecularDevices,LLC. Allrightsreserved. ## AccuBlue HiRange Assay Kit # SpectraMax® Quant™ # AccuBlue™ HiRange dsDNA Assay Kit # Quick Start Protocol for a 96-Well Microplate 1. Warm assay components to room temperature. 2. Prepare the working solution by diluting the AccuBlue HiRange dye (1:100) and AccuBlue HiRange Enhancer (1:100) in 1X AccuBlue HiRange buffer and mix. 3. Pipette 200 µL of the working solution into each microplate well. 4. Pipette $1 0 ~ \\mu \\iota$ of the SpectraMax Quant AccuBlue HiRange dsDNA standards into the microplate wells and mix. 5. Pipette $1 0 ~ \\mu \\iota$ of each unknown DNA sample into separate microplate wells and mix. 6. Incubate the microplate at room temperature for 5 to 10 minutes in the dark. 7. Measure fluorescence in a fluorescence microplate reader $E X = 3 5 0$ nm, $E M = 4 6 0$ nm). Table 1-1: Preparation of dsDNA Standards | | | | | | --- | --- | --- | --- | | Std. | Final Conc. | Std. Volume | AccuBlue HiRange Buffer Volume | | A | 200 ng/μL | 100 μL of 200 ng/μL AccuBlue HiRange Standard | None | | B | 150 ng/μL | 75\_μL of 200 ng/μL AccuBlue HiRange Standard | 25μL | | C | 100 ng/μL | 100 μL of 200 ng/μL AccuBlue HiRange Standard | 100 μL | | D | 50 ng/μL | 100 μL of 100 ng/μL of C | 100 μL | | E | 25 ng/μl | 100 μLof 50 ng/μL of D | 100 μL | | F | 12.5 ng/μL | 100 μLof 25 ng/μL of E | 100 μL | | G | 6.25 ng/μL | 100 μLof 12.5 ng/μLof F | 100 μL | | H | 2 ng/μL | 32 μL of 12.5 ng/μL of G | 68μL | | 1 | 0.2 ng/μL | 10 μLof 2 ng/μLof H | 90 μL | | 」 | 0 ng/μL | None | 100 μL | Complete instructions for using the SpectraMax Quant AccuBlue HiRange dsDNA Assay Kit can be downloaded from the Knowledge Base on the Molecular Devices support web site: [www.moleculardevices.com/support](http://www.moleculardevices.com/support) # Contact Us Phone: +1-800-635-5577 Web: Email: Visit our website for a current listing of worldwide distributors. ## Assay Kits Overview ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/7172b19268e7659bee68e1145441bb62a58765938e297ea33a4fb2f2cc40c903.jpg) # Assay kits for the ImageXpress Pico system Easy-to-use, reliable EarlyTox™ Cell Viability Assay Kits for ImageXpress® Pico Automated Cell Imaging System These assay kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and apoptosis events using mammalian cells. The kits employ a no-wash, homogenous assay protocol that enables characterization of a full concentration-response profile of test compounds. | | | | | | | --- | --- | --- | --- | --- | | Assay kit | Application | Biological marker | Key component | Part number (\*Explorer/Bulk) | | EarlyTox Live/Dead Assay kit | Live/Dead cell assay | Plasma membrane integrity | Calcein AM, Ethidium Homodimer-ll | R8340/R8341 | | EarlyTox Live Cell Assay kit | Live cell/cell proliferation assay | Plasma membrane integrity | Calcein AM | R8342/R8343 | | erlyoxCaspase37Nucvie488 | Cpoptsi 3/7 ay | Caspase 3/7 activity | NubVviewe in PCaspase-3 Masking reagent | R8350/R8351 | | EarlyTox Caspase-3/7-D NucView 488 Assay kit | Caspase 3/7 apoptosis assay | Caspase 3/7activity | NucView 488 Caspase-3 Substrate in DMSO; Masking reagent | R8348 | \*Explorer kit is sufficient for two 96-well plates and bulk kit is enough for ten 96-well plates. # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ## Digital Confocal Imaging ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/b2d32261f5805aad3b3ac96c54560c9dfba4aed0b44574ae89d78d8522414aa5.jpg) # For the ImageXpress Pico System CellReporterXpress Image Acquisition and Analysis Software Designed for te ImageXpress@ Pico Automated Cel Imaging System,and utilizing CellReporterXpress@ Image Acquisition and Analysis Software,the Digital Confocal\* Option provides 2D on-the-fly deconvolution and helps to restore the image by mathematical debluring of the image by reversing the point spread function. The Digital Confocal Option for CelReporterXpress enhances contrast of images during acquisition,allowing you to decrease exposure time more than two-fold while maintaining assay quality.Alternatively,assays with low $z '$ -factors can be improved more than $2 5 %$ ： ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/89d166d3d372b7b338608d7208259a2251b7349c259313c80c4a318cc4d52ae7.jpg) The speed and convenience offered by the new module delivers a real advantage for scientists as it provides the user with on-the-fly deconvolution without the need to post-process the images. Simply adjust the slider bar to get the desired image restoration. The numerous optical parameters required for the calculation are pre-enterd into the module. # Benefits · Enhance image contrast on-the-fly during acquisition · Decrease exposure time while maintainingassay quality · Improve object segmentation ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/0c2ed13eed71affba067638c1ac874947c187ffc2732c4fee1275bf36aaebeb6.jpg) # Mitochondrial granule count as a function of acquisition settings The Digital Confocal Option allows you to maintain assay quality while reducingacquisition exposure times. No Digital Confocal ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/28c39f4e6e766b71279cf378b5b6b459e61e6b6f12c1798687429e6ef9ede1d6.jpg) With Digital Confocal Images with Digital Confocal Option enabled during acquisition (right) show cell structures more clearly than without (left),enabling more robust image analysis. # Segmentationmask # FITC 300 ms ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/b26d6e5afa37b042ccda3015c92763d248607158c608414f2fe0da4ed4092600.jpg) Mitochondria fluorescent channel With the baseline condition of Digital Confocal off, a mitochondrial granularity count was performed with a 300 ms exposure in the FITC channel. # FITC 150 ms For sake of comparison, the exposure time was cut in half resulting in a significant loss in the mitochondrial granule count. # FITC 150 ms By turning on the Digital Confocal feature and keeping the shorter exposure time, the mitochondrial granule count isrestored. \*ImageXpress Pico Digital Confocal uses AutoQuant 2D Real Time Deconvolution # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Blgim,nmrk,iad,race,ermnyrend,therlad,pin,wededid ## Automated Cell Imaging System # Automated Cell Imaging with the lmageXpress Pico System High-throughput, remote access capable imaging for increased productivity and reduced cost # Tailored laboratory automation solutions withrobotics, incubators,and software Molecular Devices provides full-service,customization, and automation to meet your research needs.Our team includes scientists and engineers who can customize our instruments,aswellas automate entire workflows to meet the specific needs of your assay,method,or protocol.We take a consultative approach to understand your application requirementsand recommend labware,automated robotics, and software solutions that best match the unique needs of yourapplication and workflow. The research environment is constantly changing and today's scientists require simplified remote access and enhanced laboratoryautomation.Increased productivity,reduced costs, and consistent performance are all available when combining the ImageXpress? Pico Automated Cell Imaging System with the S-LAB plate handler from PAA,and the SCILA incubator from Inheco\*. The ImageXpress Pico System offers laboratories increased throughput while combining high-resolution imaging with powerful data analysis.This robust high-content imager contains over 25 comprehensive preconfigured analysis protocols,enabling you to run fluorescence imaging or brightfield assay samples,and allows you to go from sample to results in minutes.Fully integrated with an Inheco SCILA 4-Position random access ${ \\mathsf { C O } } \_ { 2 }$ incubator, you can expand the boundaries of your research to include multi-plate live-cell assays including kinetic assays. # KEY FEATURES ·Affordable, customizable solution to meet your highthroughput, remoteaccess capable,research needs · Automated plate handler increases productivity ·Quick recovery incubator to maintain optimal environmental conditions ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/85c3e689e88839cda3674c603893ceb0891aba3a205d33967222427f063b6492.jpg) The lmageXpress Pico System shown with the S-LAB automated platehandler from PAA,and the SCILA4-Position incubator from Inheco\*.Additional configurations available. # Automated plate handling Increase the capabilities of the ImageXpress Pico System by pairing it with the S-LAB plate handling system. The S-LAB plate handling system is an easy-to-use compact automation solution designed for ambient and incubated cellular assays. The S-LAB plate handler integrates seamlessly with the ImageXpress Pico System, offering unattended loading and unloading of plates. Equipped with a barcode reader and delidding functionality as standard, the S-LAB plate handler offers a reliable,flexible,and easy-to-use automation solution with full plate tracking and LIMS integration. Assay Managerm is the intuitive easy-to-use software solution for running multiple kinetic assays. It enables multiple users to queue their samples for processing, even when there are batches of plates being processed. Reads will be scheduled to run even when the system is unattended. Simply answer a few questions about your assay, load the plates, hit‘go' and walk away. Assay Manager turns operating an automated imaging workcell into a simple procedure,requiring minimal training. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/1016f764883bf4eeb8cb8d6d7c43245396a0321ee77b23e2cf437a3240ac8f39.jpg) # Automated cell culture applications The ImageXpress Pico System integrates with the SCILA 4-Position ${ \\mathsf { C O } } \_ { 2 }$ incubator from Inheco allowing you to accommodate extended live-cell experiments. Temperatureaccuracyof $+ / .$ $\\Im \\mathsf { C }$ at $3 7 ^ { \\circ } \\mathsf { C }$ and uniformity from drawer1to 4 creates a humidity ventilation circle, eliminating the need for a fan ensuring quiet continuous operation. Fast random access ensures quick recovery of the incubation conditions to ensure optimal environmental conditions for humidity, temperature,and gas atmosphere. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/7994c87f774d7e15b55fa194c91a7c48ad237ef5bde663ddb652124610114acc.jpg) # Customization and automation We areradyto help you solve yourtough research chalenges.Our proven solutionsand highly qualifiedteams across the globe can help advance your next big discovery. Please contact us to discuss your research needs. \*CustomolutdleissbecturtdctcTrsbtoeuladeoutprducts Highligteduitiildts cause adjustment to standard performance. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listingofworldwidedistributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,irkdsn ## Water Immersion Objectives ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/8575e267407842940f2d035174b620641fb1d8ff312fff98d4a170aafd3bef40.jpg) Gain the sensitivity to capture more phenotypic data at greater depths. Our high-content imagers and expert team are here to help your lab simplify imaging and analysis of complex, 3D assay workflows and fast-track your discoveries. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/077ae2133e9557309c3333b4301495d664580b143610c2e9e8ced8c1d1b50f9c.jpg) # Gain up to 4 times more signal at depth Improve z-resolutionand decrease optical aberrations formoreaccurate reconstruction of 3D samples. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/1ca180873b30becb86a609a38f83892e0c5ee9f7e6ba36921a7079b202541eee.jpg) # Gain sharper, crisper images Better light collection results in images with brighter intensity at lower exposure times,contributing to better quality of data. # Benefits · Increase in penetration depths dependent on sample · Improve z-resolution and decrease optical aberrations · Gain sharper, crisper images · Acquire 3D samples at high throughput · Shorten image acquisition time ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/c652e59be44eed749c199eab87ea2ef5bf277b9a51751aaf014403815cb52d1d.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/6c30a16c691439568343048db389d413c5f5e8d9d6912b2ae932ea3a6a9329b1.jpg) # Streamline set up to get started more quickly Water immersion objectives are field upgradeableand can be implemented with minimal change to current workflows. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/de8a18e7b0be33cbf6d7d18ae38d6e03ee3c56094a05030f2b821ee0f5404bd4.jpg) # Run your samples with confidence MetaXpress software includes integrated, user-intuitive sensors and alerts to indicate the system health of the water controller system to give you peace of mind when running your samples. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/5cfd8abd444222f0273ca9ca6ab05c3a56d11181cdb506e0d5aab20040470d1a.jpg) # Acquire 3D samples at high throughput The ImageXpress Micro Confocal system with water immersion objectives automatically replenishes the water duringacquisition, so the entire microplate sample can be acquired at high throughput. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/459f8323c07359dc5805576fbe17f0e93e1c6f66d34aa6c4af60e14f2689dab3.jpg) # Simplify viewing and quantitation of 3D structures Analyze spheroids,microtissues,cells ina 3D matrix,and small organisms that are acquired as a stack of z-planes. Utilize the MetaXpress 3D Analysis Module to evaluate volume, $x \\mathsf { Y } Z$ location,distance to neighboring objects, diameter, depth,various intensity measurements, texture,or number of objects. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/ead231d71438a583cd961283d384f13b7816deeb0af0d5966568982be929d478.jpg) # Shorten image acquisition time QuicklD targeted acquisition images at low magnification to find objects of interest or rareevents, then,automaticallyacquires high-magnification images with the required wavelengths,z-stack,or time points.This analysis process shortens acquisition time and reduces imaging storage requirements by acquiring selected targets at high magnification. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/00d62568ca609588e3199460a88602ea5e798d5ec924c4533e1d3f7188f40eb6.jpg) | | | | --- | --- | | Specifications | | Available water objectives | 20X,40X,and 60X (up to 1.2 NA) | | Signal (Brightness/intensity) | Increase up to 4 times | | Auto-water replenishment | Yes | | Automation-friendly | Yes | | Additional options | Transmitted Light Tower, Environmental Control | | | | | --- | --- | | Available high-performance customizations+ | Laser Light Source, Deep Tissue Penetrating Confocal Spinning Disk Module | | Field upgradeable | Yes | | Compatible systems | ImageXpress? Micro Confocal High-Content Imaging System | +For terms and conditions, please visit moleculardevices.com/ixmc#options # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan (Osaka) +81.6.7174.8331 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 Japan (Tokyo) +81.3.6362.5260 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 South Korea +82.2.3471.9531 \*Austria,Belgim,mrk,id,rae,Germny,eld,thers,pinwdendi ?2020 Molecular Devices, LLC 2/202303B Printed in USA ## ImageXpress Nano System ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/ea34bafea4d24adb9d5f5d8107903557fad80eef5b0545e7a440458cdeb38a56.jpg) # ImageXpress Nano Automated Imaging System # BENEFITS • Integration into MDCStore Data Management tool for easy access to needed files • Bidirectional image viewer facilitates data quality control by connecting images to numerical data • Multi-parametric analysis and visualization • Dose response analysis and curve fitting with over 30 different predefined functions # Acquire Widefield screening with automated microscopy allows researchers to scale up their experiments to multi-slide or microplate-level evaluations. The ImageXpress® Nano Automated Imaging System features a long life, solid state, light engine, and optics to reliably deliver the right assay sensitivity. # Analyze Expedite image analysis and data processing with application modules that allow you to quickly and easily analyze your data. Our modular toolbox set up hundreds of routinely used HCS assays whether you’re a novice or an expert. MetaXpress® software application modules give you an interactive environment to quantify 2D and 3D measurements including size, intensities, and distance. # Intelligent segmentation The MetaXpress software application modules are equipped with a proprietary adaptive background correction algorithm, which adjusts image segmentation to the local intensity ranges and shape features within and between cells. This capability gives you the most robust segmentation available in an image-based screening system and improves detection on both phase contrast and fluorescent labeled images. # Software features ntegration into MDCStore™ Data Management tool for easy access to needed files Bidirectional image viewer that facilitates data quality control by connecting images to numerical data • Multi-parametric analysis and visualization Dose response analysis and curve fitting with over 30 different predefined functions ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/a8381cb55f43aec3b37516dc5c800bd293acca335b2044ecc3dea367de273375.jpg) Autophagy ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/d6e9649fdff8c2e09b119c896335bf32373c0d5794642e17a9c688b7bf56d80d.jpg) 3 color toxicity # System features Widefield imaging • L arge field of view (A single $4 \\times$ image captures whole 384 well) Solid state light engine with expected lifetime of 20,000 hours Acquire from a variety of multi-well plate formats and large format samples (e.g. slides) 4 position software-controlled objective changer • 5 position software-controlled filter cube changer • High speed laser autofocus technology • Fully automated X, Y, and Z stages with resolution better than $2 5 { \\mathsf { n m } }$ ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/3332720087bfabce0d2d90c9b038478a276c64531e320629326fb5bf434374f6.jpg) Intracellular vesicle mask ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/ace3d50e9e06befd4174220179e2eeefb01a6afccc3521c67771d34a6ea56d58.jpg) Neurite tracing # Options ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/9eaba043dd78dd864107cc71506d5f442350d03b640a164a6451fa10ed430b8b.jpg) Nuclei and cytoplasm mask • Transmitted light for label free brightfield imaging Cell incubation (temperature, humidity, ${ \\mathrm { C O } } \_ { 2 } ^ { \\prime }$ ) with environmental control feature Desktop computer with pre-installed software • Objectives: $2 \\times$ to $6 0 \\times$ magnification • Select from a variety of filter cubes ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/51638299fff6dcb4f3de18a73f5c3c0ad265b4210c50b6f195ed69e41e430dc2.jpg) Transfection efficiency with overlay # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/2be00abf7267e649f6e6b2750c9ac9b2edf910ad269e83541e3e420c0dc90434.jpg) ## ImageXpress Micro Confocal ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/01bc2282d40b10b557bbfa557d9f5b7b9ec05b413bde79a4db331d669ab77d2c.jpg) # ImageXpress Micro Confocal High-Content Imaging System # The confocal solution for your complex biology # Higher quality images, faster throughput, and more powerful analysis The lmageXpress@ Micro Confocal High-Content Imaging System provides improved quantification for live or fixed cell assays.This versatile imaging system features a unique confocal technology which allows you to explore more physiologically relevant, complex 3D models including spheroids, tissues,and whole organisms and to generate publication quality images at high throughput for samples in slides or one to 1536-well microplates. For researchers looking to expand their laboratory's capabilities,the ImageXpress Micro Confocal system leverages large field-of-view optics to map macrostructures with minimal tiling.In addition, querying of large cell populations is accelerated,speeding up the characterization of highly heterogeneous samples or identification of rare subpopulations. # Key features · Acquire statistically relevant data quickly with an advanced scientific CMOS detector, enabling $^ { > 3 }$ log dynamic range · Improve visualization and quantitation with 3D assay models · Achieve excellent image quality without sacrificing throughput using our unique optical path technology · Expand your research capabilities with water immersion objectives, transmitted light, phase contrast optics, on-board liquid handling, and environmental control options ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/0e361dd3d1567ce0ed6c895b9c77605e4c2aa447cec78846baf601c5efc22e7e.jpg) Combined with MetaXpress? High-Content Image Acquisitionand AnalysisSoftware,the ImageXpressMicro Confocal systemisacomplete solution that enables you to interpret your images, understand your data, andexplore new ideas-in both widefield and confocal modes. # Specifications # System ·High-speed laser autofocus with integrated image autofocus option ·Linear encoded voice coil driven X,Y,and Z stages with better than 25 nm resolution · 4-position automated objective changer\* ·5-position software selectable dichroic filter wheel\* ·8-position software selectable emission filter wheel\* ·Sample compatibility: slides and one to 1536-well microplates, round or flat bottom, low to high profile,and Transwell? plates # AgileOptix optical path ·AgileOptix" technology enables the ImageXpress Micro Confocal system to deliver the sensitivity and throughput needed for demanding applications by combining a powerful solid-state light engine, high-quantum efficiency 16-bit, ${ > } 4$ megapixel scientific CMOS sensor, and selectable unique confocal geometries · $^ { > 3 }$ log dynamic range is available in both widefield and confocal modes ·Large fieldof view ( $1 . 9 6 ~ \\mathsf { m m } ^ { 2 }$ at $1 0 \\times )$ imaging maximizes collection of publication quality images and statistically relevant data \*User changeable ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/17b21ea0c1259259fac93853f654ec675cb5b6127c6c904ffe79ccceb1c2a556.jpg) Acquire greater than 2oo,ooo wells per day at 4X magnification with the system's unique multi-well crop feature. | | | | --- | --- | | Option Feature | | | Water Immersion Objectives | ·20X,40X,and 60X (up to 1.2 NA) ·Increase signal up to 4 times for brighter intensity at lower exposure times ·Increase in penetration depths dependent on sample ·Improve z-resolution and decrease optical aberrations ·Auto water replenishment enables screening or imagingacrossa plate | | Environmental Control | ·Multi-day,live cell time-lapse imaging ·Provides appropriate atmospheric conditions (e.g.5% or 10% CO2) ·Mimics physiological environment (30-40C±0.5C) ·Controls humidity and minimizes evaporation (0.5 μL/well/hour for 96- or 384-well formats) | | Phase Contrast | are easily viewed or separated from background (4X-60X) ·Ideal for non-fluorescent histochemically stained samples ·Nikon 1OoW Pillar Diascopic Illuminator with TE-C ELWD Condenser · 0.3 NA with 65 mm WD and PhL, Ph1,and Ph2 selectable phase rings ·Fluorophore-independent morphology visualization with fluorescent imaging overlay | | On-board Fluidics | ·Single-channel pipettor ·Dispense volumes from 3 μL to 20O μL (±1 μL; ±5%) ·Compatible with 96- or 384-well format FLIPR System pipette tips ·Holds two plates for compound addition or media exchange ·Optional plate heating | Note:all options,filters,and objectives are available at point of sale or as after market upgrades. Configurations shown herein do not encompass all configurations available. Contact your sales and support team today to identify the system configuration most suitable for your applications. # Implement a solution that works for you Molecular Devices can successfully tailor the lmageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described herein,aswell as integrationof other lab components such as incubators,liquid handlers,and robotics for a fully automated workcell.With over 3O years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support. Sale is subject to our Custom Product Purchase Terms available at [www.moleculardevices.com/custom-products-purchase-terms](http://www.moleculardevices.com/custom-products-purchase-terms). # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan (Osaka) +81.6.7174.8331 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 Japan (Tokyo) +81.3.6362.5260 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 South Korea +82.2.3471.9531 \*Austria,Belgim,mrk,id,rae,Germny,eld,thers,pinwdendi ## MDCare Support Plans ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/80ee813a4af214a0a3f09fbdfc5d8b3cc89d1ab416e0b3b70ce5bbbc01772ac2.jpg) # MDCare Support Plans # For high-content imaging systems Withthe MDCare"Support Plan,you willhave priority access toour experienced ImageXpress@/MetaXpress? Technical Support Team.Aone-year subscription toour MDCare PhD Plan is icluded in te purchase price of allhigh-content imaging instruments.The MDCare PhDplan provides assurance thatwe willbe here to help you when it's most important. Year 1 Instrument warranty and MDCare PhD Plan included in year 1 Years 2-3 Extended technical support can be purchased through any of our MDCare Plans | | | | | --- | --- | --- | | MDCare PhD Plan | MDCare Tailored | MDCare One | | Key benefits include: | Initial consultation included with MDCare | Single incident coverage that includes: | | · Up to 20 hours/year of live support (90 minutes per incident): | PhD Plan: · One-hour consultation about your specific assay | · Priority response | | · Software installation | ·Estimate on costs to implement solution | · Up to 90 minutes of PhD qualified active support ·Assay-specific analysis support | | ·Data acquisition | Essential development: | · Software installation | | · Autofocus optimization | · Tailored solution designed for your assay | ·100% of value can be credited towards MDCare | | · Assay-specific analysis support, and more | · Custom acquisition protocol | PhD Plan within 15 days | | · Priority response | · Tailored analysis routine | | | · Access to a highly qualified team specializing in | ·Image and data management | | | solving advanced imaging problems · Dedicated support to optimize your imaging | ·Confidently move from assay development to screening | | # Contact Molecular Devices Technical Support at 1.800.635.5577 (option 3) or [support@moldev.com](mailto:support@moldev.com) for more information and program details. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria,BikdedIlytnd ## Digital Confocal Option ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/cd2d740292046e0a9177b730b4a08dc665d9cee49a3fa91c72a143f9b3a67d7c.jpg) # Digital Confocal Option # For MetaXpress High Content Image Acquisition and Analysis Software Designed for use as part of MetaXpress? software version 4 and greater, the Digital Confocal+ Option significantly increases throughput while maintaining assay quality. The Digital Confocal Option for the ImageXpress? Micro imaging systems enhances contrast of images during acquisition,allowing you to decrease exposure time more than two-fold and increase throughput while maintaining assay quality. Alternatively, assays with low $\\vec { z } ^ { , }$ -factors can be improved more than $2 5 %$ .This intelligent analysis enables researchers to increase their throughput, allowing acquisition and analysis of more than 1O million cells per hour. The speed and accuracy offered by the new module deliver a real advantage for scientists working in high-content screening, where significant resource is dedicated to screening millions of samples for rare new drug or gene therapy candidates. The integration of ImageXpress Micro systems with the Digital Confocal Option for MetaXpress software provides unrivaled performance for ease-of-use,while providing high speed and quality images researchers can be confident with. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/2117794f7d7b25f754c3fb6e1194dd8c64a8ed7efb2394ebe8ad7f68027cb5b7.jpg) # Assay $z '$ -factor as a function of acquisition settings The Digital Confocal Option for MetaXpress software allows you to maintain high Z'-factors even while significantly speeding up your acquisition. # Benefits · Enhance image contrast on-the-fly during acquisition · Improve object segmentation · Decrease exposure time while maintaining assay quality ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/cf0a7f0a0fc2a0cd41d8238f3a11a0b5f76b21d291527cc1fd2d1e575460baff.jpg) Images of U2OS cells stained for tubulin were acquired without (left) and with (right) the Digital Confocal Option enabled.Analysis of digital confocal images allow bettercharacterization of microtubules. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/d8e8397a98dfdc965573f99c3cc53d2f0be78e0da4a1b47072467f3bd6a17f54.jpg) The overall file size of the digital confocal images (right) is no different from images saved with standard widefield settings (left). # AutoQuant +Using AutoQuant 2D Real Time Deconvolution integrated innovation Images of HEK 293 cells containing paraspeckles were acquired with the ImageXpress Micro system without (top) and with Digital Confocal Option (K value 0.063) (bottom). The histograms of a line scan through representative images shows how the Digital Confocal Option can sharpen the intensity peaks of the fluorescent objects.A more accurate count of the paraspeckles wasachieved when analyzing the digital confocal images with the Granularity Application Module for MetaXpress software (right). # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listingofworldwidedistributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Bgimrkid,rae,ryed,trsindd ## Organoid Innovation Center ORGANOIDINNOVATION CENTER # Quickly adopt innovative 3D biological methods and technologies for drug discovery The Organoid Innovation Center showcases cutting-edge instruments that work harmoniously together for autonomous,long-term,3D and 2D live cell culture growth and monitoring with intelligent imaging. This integrated workflow provides quality control alerts and readiness,3D organoid screening,and deep learning image analysis that reveals hidden patterns other technologies miss. The end-to-end solution standardizes the organoid development process by growing complex 3D cell cultures (incubation,treatment, passaging,and monitoring)and the downstream analysis capabilities utilizing confocal imaging,analysis,and data processing. The Organoid Innovation Center delivers consistent,unbiased, and biologically-relevant results at scale. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/bfc0de0b0193421b19d0e750da5623dfc3f768873ad4051897a0a8396b74ee70.jpg) # See the power and flexibility of an automated and customizable high-throughput screening solution ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/3540d54235825c73815a86b6f134d9959d303ac402b87d7d9638fbc461b5ab3c.jpg) Automated CO2 incubator (LiCONiC Wave STX44) Want to know more about automating your organoid screening workflow? Visit moleculardevices.com/organoid-innovation-center # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/d07bb1c4acf7330e63c08bd7fd10848f15fed997807aaa4c9e754966058dbfd2.jpg) USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China +86.4008203586 Taiwan/Hong Kong +886.2.2656.7585 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 India +91.73.8661.1198 ## MetaXpress 6 Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/8796e7dbcf4b269219caa49753fcc0002843547f3fd43258c06de5f1bf382294.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/5d88ff72b1a04f5aabbc111534820b88d29247dc19059f079429b92366238927.jpg) # MetaXpress 6 Software Transform your high-content imaging workflow # BENEFITS • Integrated Z-stack acquisition and analysis • Faster-than-acquisition 2D Z analysis enabled by MetaXpress PowerCore software • 3D analysis module simplifies 3D data quantification and throughput • New peak analysis for cardiac beating assays and curve fitting # Simplify workflow Reimagine your current workflow with MetaXpress® High-Content Image Acquisition and Analysis Software. The latest layout simplifies plate acquisition setup by consolidating key functions in one place. Always visible and always accessible plate layout views make acquiring your data easy and quick including the capability of appending time points to already acquired plates for asynchronous kinetic experiments. # Intuitively set up 3D acquisition Easy-to-use graphical interface enables you to rapidly set up 3D imaging. Interactive tools help you determine optimal settings for Z acquisition. Save images from each and every plane or use best focus, maximum, minimum, or sum software algorithms to generate and save only a single 2D projection image. # Customize analysis with ease Extend your assay capabilities as your needs grow and change using Custom Module Editor. Quickly render and record 3D visualization of raw data and segmentation as well as quantify your 3D structures with measurements such as volume, intensity, distance and more. # Strong foundation, great new features | | | | | | --- | --- | --- | --- | | Key features | Versions 3& 4 | Version 5 | Versions 6 & 6.5 | | MetaXpress? Acquisition and Offline Analysis Software | | Time-series acquisition and analysis | With Journals | Integrated with Acquisition and Analysis Wizards | Integrated with Acquisition and Analysis Wizards | | Z-series acquisition | With Journals | With Journals | Integrated with Acquisition Wizard | | Z-series analysis | With Journals | With Journals | ·Integrated into workflow ·Compatible with MetaXpress PowerCore software | | Customized analysis 3D analysis | With Journals N/A | External Custom Module Editor N/A | Integrated Custom Module Editor Available within Custom Module Editor | | Transmitted light segmentation | With Journals | With Journals | Simple step cards available with Custom | | Asynchronous kinetics | N/A | N/A | Module Editor (6.5) Integrated with Acquisition Wizard (6.5) | | Basic curve fitting | With AcuityXpress software | With AcuityXpress software | Available with MetaXpress software (6.5) | | Peak analysis | N/A | N/A | Available with MetaXpress software (6.5) | | Windows compatibility | Windows XP | Windows 7acquisition and analysis | ·Windows 7 acquisition and analysis ·Windows 8 analysis ·Windows 10 acquisition and analysis | | MetaXpress? PowerCore" Software | | Scale analysis to be faster than acquisition | Single time point and plane | Including time-lapse | Including time-lapse, multiple z planes,and | | Run multi-core analysis on acquisition computer | No | No | custom modules Yes | Windows 10 acquisition for ImageXpress Micro XLS, ImageXpress Micro 4 and ImageXpress Confocal Systems. Windows 10 acquisition computer must be provided by Molecular Devices LLC. # Application modules We address hundreds of image-based analysis scenarios. Our turnkey application modules tackle your basic tasks. With MetaXpress Custom Module Editor, you are empowered to tailor image analysis to your research needs. | | | | | | --- | --- | --- | --- | | Application modules | Part number | Applicationmodules | Part number | | Custom Module Editor | 9500-0158 | Mitotic index | 9500-0036 | | 3D analysis (requires Custom Module Editor) | 5047663 | Micronuclei | 9500-0045 | | Curve fitting | 5058772 | Monopole detection | 9500-0039 | | rai | 5058774 | | 950004 | | Angiogenesis tube formation | | | | | Cell cycle | 9500-0016 9500-0040 | Neurite outgrowth | 9500-0015 | | Cell health | | Transfluor@ assay | 9500-0033 | | Cell scoring | 9500-0035 9500-0037 | Translocation (includes Translocation-Enhanced) | 9500-0014 | | Count nuclei | 9500-0017 | Transfluor HT assay | 9500-0043 | | Granularity | 9500-0032 | Nuclear translocation HT | 9500-0041 | | Live/dead | 9500-0034 | Cell proliferation HT All MetaXpress software application modules | 9500-0042 5062036 | # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ## Neurite Outgrowth Module ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/0a2b1e4a4999f99bf90d9f1156892c3fdf44800236d07028a59e0ad50734a590.jpg) # Neurite Outgrowth Application Module For MetaXpress High-Content Image Acquisition and Analysis Software # BENEFITS • Works with or without fluorescence staining • Superior segmentation algorithms and adaptive background correction • Accelerate analysis with MetaXpress PowerCore HighContent Distributed Image Analysis Software Run module over timelapse images Neurite outgrowth is the extension of axonal processes from the cell body and a natural part of development. Inhibition or stimulation of neurite outgrowth is implicated in a broad range of CNS disorders or injuries including stroke, Parkinson’s disease, Alzheimer’s disease and spinal cord injuries. The Neurite Outgrowth Application Module for MetaXpress® software is designed for the analysis of neurite outgrowth assays. The module helps standardize results compared to traditional methods. Using a nuclear stain is beneficial to identify cell bodies in some cell types. With the flexibility of MetaXpress software, researchers are able to choose whether or not to use nuclear stains. The use of a nuclear stain adds extra assurance in achieving a one-to-one correlation between nuclei and cell bodies. The module utilizes adaptive background correction, which adapts the detection of features to the local intensity ranges and shape features within and between cells to provide robust segmentation available in an image-based screening system. A simple interface minimizes setup efforts. Analysis settings can be configured once and saved for future use or customized to fit a specific experiment. # Easy configuration for analysis 1. ``` Select the neurite image of interest ``` 2. ``` Specify the illumination (transmitted light or fluorescence) ``` 3. ``` Set the classification limits for cell bodies ``` 4. ``` Select classification limits for optional nuclear stain if desired ``` 5. Choose desired parameters to report # Interactive data display Once the analysis is run, the results table allows you to interactively view individual cells’ data. Clicking a cell in the image highlights the data for the selected cell in the results table. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/15764481fafd2da76f2ee2ccdee3813e68fbea17842c9065259cae5d0ca0c738.jpg) Image segmentation. Untreated neurons (top) have many long outgrowths compared to $1 0 \\mu \\mu$ Staurosporine-treated (bottom) after overnight acquisition on ImageXpress® Micro system using transmitted light. Multi-colored mask shows image segmentation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/58135b3ae93e6b7d2237f18cef5d680f855e89f49cc2083640e0c12a7085d9fc.jpg) Time vs. well heatmap. Change in neurite outgrowth visualized through heat map over 36 time points. A05 and A06 wells contain neurons treated with staurosporine. Other wells contain untreated cells or cells treated with a growth factor. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/210e5d484f479917639016f400de7ac320574201318fa58587e3b2c53bb10092.jpg) Data visualization and analysis. Data visualization with AcuityXpress High-Content Informatics Software. A higher percentage of cells had significant outgrowths on untreated vs. Staurosporine-treated cells. # Contact Us Phone: $+ 1$ -800-635-5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. # Multi-parameter analysis Field measurements include, but are not limited to: • Total cells, outgrowth, processes, branches, and cell body area • Mean outgrowth, processes, branches, and cell body area • Straightness of processes • Significant growth Cell-by-cell measurements include, but are not limited to: • Outgrowth • Processes • Mean, median and maximum process length • Mean outgrowth intensity • Branches • Straightness of processes • Cell body area # Time-lapse analysis The Neurite Outgrowth Application Module for MetaXpress software can be applied to analyze images acquired over a time course. Simply setup segmentation parameters and begin analysis on select wells of a plate or batches of multiple plates. The Time vs. Well Data View allows the user to scroll through data per time point per well in a heat map format. # Further customization through macros MetaXpress software and the Neurite Outgrowth Application Module are seamlessly integrated with the flexibility of MetaMorph® software and its advanced automation macros. These powerful macros record and perform a series of tasks without the user having to know a programming language. # The fastest workflow from acquisition to hit selection Image acquisition with ImageXpress automated imaging systems provides the fastest read times for large libraries and great flexibility in acquisition setup for research through screening. On-the-fly cell counting ensures a predefined number of cells is captured and decreases image acquisition times. Image analysis can be distributed to the high-throughput image analysis platform, MetaXpress PowerCore™ software, providing unparalleled performance in the race to identify “hits.” Increasing image analysis speed by 10- to 30-fold virtually eliminates image analysis bottlenecks and enables multiple ImageXpress systems to run in parallel. High-end hit selection and quality control is available through AcuityXpress™ software, the data analysis software integrated in our complete imaging solution. AcuityXpress software features an interactive drill down for bidirectional interaction between images and numerical data. ## ImageXpress Pico System # MOLECULAR DEVICES # ImageXpress Pico Automated Cell Imaging System Compact system with intelligent image acquisition and analysis # Automated acquisition and analysis of cell images Replace tedious manual microscope manipulations with the fully automated ImageXpress@ Pico Automated Cell Imaging System.Simply place samples into the system and follow the icon-driven,step-by-step workflow to capture and analyze images.The system software features over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing,removing the guesswork from optimizing parameters.Results can be visualized in various formats including heat maps,scatter plots,tables,barcharts,andmovies. Place sample into the system ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/3cff7b8b71d60f714c3dd3be346ce38ad2a4d6d2b762a8011c30f368acafcf2d.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/f751237a78efa42280960b2e5e68f830266ceb49ad44245f50eb8fddc23eb946.jpg) # Automatically capture and analyze images ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/6553fce4e5626ca94607a5cd5def2d5409f17ac5f29f46ad60771b014adbaf81.jpg) Segment cells/objects ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/a70a1d7341f0f8c5a5a23bbc8ddc03009671e3f397e7581185505811fddf588d.jpg) Review thumbnail images ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/3a6a9f1ea7d2b18b9636ad53594d662850e5aa0d4a9aab0254cfa78b46958361.jpg) Scatter plots # Key features · Minimize experiment set-up time using optimized,preset acquisition and analysis modules for fast and accurate assay results · Easily generate heatmaps,scater plots,and bar charts from analyzed image data in just a few clicks ·Enhance contrast of images during acquisition with the Digital Confocal\* 2D on-the-fly deconvolution option, increasing resolution and improving assay quality · Perform live cel experiments while maintaining optimal conditions with environmental control · Generate sharper images for more accurate segmentation using z-stack acquisition # Significantly increase resolution Significantly increase resolution and assay quality with Digital Confocal\* 2D on-the-fly deconvolution.The Digital Confocal Option restores light to its original point of origin,allowing you to decrease exposure time and improve statistical significance of your observations.Digital Confocal is seamlessly integrated into the ImageXpress Pico's fluorescent image acquisition workflow allowing you to capture images with higher signal-to-noise data for more precise segmentation and analysis. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/e0b78bdd36336bfcfacab7b931ba85874dbf80d07a4de3e0ae8267fb6f7b1213.jpg) Specifications | | | | --- | --- | | Modes of operation | White light/brightfield, colorimetric,fluorescence, Live Preview | | Objectives | 6 position automated turret with user- exchangeableobjectives.OpticsbyLeica Microsystems: 2.5X N Plan/NA 0.07, FLUOTAR 4x/NA 0.13,10x/NA 0.32,20x/NA 0.40,40x/NA 0.60,63x/NA 0.70 | | Channels | Cy5, TRITC, FITC, DAPI, Texas Red, CFP, white light, and RGB | | Imaging method | Single color, multi-color, time-lapse, and z-stacking, digital confocal 2D on-the-fly deconvolution\* | | Autofocus method | Hardware or Image with hardware assist | | Supported labware | 6- to 384-well plates, slides,and 35 mm culture dishes | | Supported operating systems | Windows 10 (main computer), Windows 10 and macOS (clients) | | Dimensions (cm) | 45.3 (H)× 55.1(W)× 43.5 (D) | | Weight (kg) | 38 kg including options | | Temperature control | Ambient+8C to 40C | | Temperature control homogeneity | 37℃± 0.5C at23C ambient | | Gas control | O, control,1-15% and ambient, CO, cotrol, ambient to 15% | | Humidity control | Active humidity control. Sample compartment controlled to 85% nominal humidity. | The ImageXpress Pico system features optics byLeica Microsystems. \*ImageXpress Pico Digital Confocal uses AutoQuant 2D Real Time Deconvolution # For more information, please visit moleculardevices.com/pico # Contact Us # Regional Offices # Configurable options support a wide range of assays With a software-selectable range of objectives and filters, integrated white light and colorimetric imaging,and over 25 preconfigured protocol templates available,the ImageXpress Pico system has the flexibility to support a wide range of assays. Capable of generating publication-ready data in just a few clicks of the mouse, the system is an intelligent addition to any lab. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/img/images/79870c01fb1b4879249cb5d87ee1b83a1ad2d604aad0932eae4b42b44e25964f.jpg) | | | | | | | --- | --- | --- | --- | --- | | none: +1.800.635.5577 | USA and Canada +1.800.635.5577 | | Taiwan/Hong Kong +886.2.2656.7585 | | | eb: www.moleculardevices.com | United Kingdom +44.118.944.8000 | | Japan | +81.3.6362.9109 | | mail:info@moldev.com | Europe\* | 00800.665.32860 | South Korea | +82.2.3471.9531 | | heckourwebsite foracurrent | China | +86.4008203586 | India | +91.73.8661.1198 | | ting of worldwide distributors. | \*Austria,Belgium,emark,nand,Frnce,Germny,eland,Italyetherlands,Portugal,pin,de | ## High-Performance Imaging Solutions Scalable, high-performance, high-content screening solutions with the ImageXpress® Micro Confocal system Capture more data at greater depths for large 3D samples • Water Immersion objectives • 5- and 7-channel high-intensity lasers • Deep tissue penetrating, confocal disk module • Turnkey high-throughput, long-term kinetics • Real-time dose response pipettor Maximize flexibility in application workflow and experimental design Get optimized imaging capabilities for live cell imaging, hypoxia studies, thick cell samples, and multiplexed assays. SCIENTIFIC CMOS SENSOR POWERFUL SOLID STATE, LIGHT ENGINE LARGE FIELD OF VIEW OPTICS (AIR, WATER, OIL) DUAL SPINNING DISK (SLIT & PINHOLE) Expand experiment capabilities with 5- or 7-channel high-intensity lasers\*. The deep tissue penetrating, confocal disk module\* reduces crosstalk to improve out-of-focus light suppression and penetrates deeper into tissue. 20X, 40X, and 60X water immersion objectives improve the geometric accuracy during acquisition and increase numerical aperture (NA) for brighter intensity at lower exposure times. Water immersion objectives Water immersion objectives can offer signal increases of up to 4X† which can help scientists see deeper into 3D and thick tissue samples at lower exposure times. • Increase penetration depths into samples • Improve z-resolution and spherical aberrations • Gain sharper, crisper images • Access more data than ever before † Data and images were acquired during development using customer samples. Results may vary. 20X Plan Fluor Air 0.45 NA 20X Plan Apo Air 0.75 NA 20X Plan Apo Water 0.95 NA Image 0 5000 10000 15000 air Fluorair Apowater Ave Intensity Average intensity Increased average intensity in 3D assay using water immersion objective. Nuclei of spheroids at 50ms exposure, maximum projection using confocal imaging. Mask Image AirWater 0 500 1000 1500 2000 2500 cell count watercell count air Cell count max projection Cell count Increased cell count in 3D spheroid using water immersion objective. Max projection using confocal imaging. 0 500 1000 1500 2000 2500 cell count watercell count air Cell count max projection 0 500 1000 1500 2000 2500 cell count watercell count air Cell count max projection AirWater Plan Fluor Air Plan Apo Water Plan Apo Air Standard spinning disk Deep tissue penetrating, confocal disk module Images taken at the same exposure. Deep tissue penetrating, confocal disk module An upgrade to the deep tissue penetrating, confocal disk module, combined with a laser light source, can improve resolution when imaging thick tissue samples†. • Improve suppression of out-of-focus light • Reduce haze (pinhole crosstalk) • Penetrate deeper into thick tissue samples for sharper images \*High-performance customizations Molecular Devices can successfully tailor the ImageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described below, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated work-cell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support. Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms. StandardLaser Images taken at the same exposure. High-intensity lasers High output laser excitation can reduce exposure times by up to 75%.† The laser light source is available as either a 5- or 7-channel light source with outputs of 400–1,000 mW/channel. The 7-channel laser light source includes near IR and is ideal for customers with increased multiplexing requirements. • Obtain sharper images with higher signal-to-noise • Generate up to a 2X† boost in scan speed attributed to significantly reduced exposure times • Run FRET experiments using lasers for CFP and YFP † Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ©2020 Molecular Devices, LLC 11/20 2266D Printed in USA Phone: +1.800.635.5577 Web: www.moleculardevices.com Email: info@moldev.com Check our website for a current listing of worldwide distributors. \\* Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland Regional OfficesContact Us USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.3971.3530 Scale-up automation Increase throughput, eliminate human errors, maintain sterility, and achieve consistent sample handling. Modular automation design—components can be added in modules and are upgradeable. Customized light sources Improve speed and image quality with multiple laser upgrade options. Real-time dose response Automatic pipettor enables compound addition while simultaneously live streaming at >100 frames per second. Turnkey high-throughput long-term kinetics Schedule and image multiple plates over long periods of time while keeping consistent temperature, O 2 (Hypoxia), CO 2 , and humidity conditions. Expand live cell walk-away capacity to 200+ plates. \*Additional high-performance customizations Implement a solution that works for you Molecular Devices can successfully tailor the ImageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described above, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated work-cell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support. Sale is subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms. ## IonWorks Barracuda Overview ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/009fa1fdbea5660f923928a044a274dc6f5198a29c010cab026bd51115bd3b17.jpg) This document is provided to customers who have purchased Molecular Devices, Inc. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify, or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose names are registered as trademarks and/or function as trademarks of their respective owners. Any such usage is intended only to designate those manufacturers' products as supplied by Molecular Devices for incorporation into its equipment and does not imply any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks. Molecular Devices makes no warranties or representations as to the fitness of this equipment for any particular purpose and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use to which the purchaser may put the equipment described herein, or for any adverse circumstances arising therefrom. For research use only. Not for use in diagnostic procedures. CE # IonWorks Barracuda™ Automated Patch Clamp System # Daily Operation Overview 5005925 A November 2010 # IonWorks Barracuda™ System Daily Operation Overview This daily operation overview provides a summary of the basic steps required to use the IonWorks Barracuda™ instrument for a set of experiments. For specific instructions on performing each of these steps, consult the software Help or the User Guide and follow the detailed instructions carefully. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/f37741d9fed300e052b15379d130a535a33975f3f05e17ad1d063c6a20fdfa54.jpg) # Prepare the Instrument 1. Turn the system on and start the IonWorks Barracuda™ Software. 2. Prepare the system fluids. Empty waste carboys in the system cabinet below the process deck. Fill source bottles. Each experiment run uses approximately $3 0 ~ \\mathsf { m L }$ of external buffer, $5 0 ~ \\mathrm { m L }$ of internal buffer, $2 0 \\dot { \\mathsf { m } } \\dot { \\mathsf { L } }$ of cell perforation agent. Adjust the final volume based on the total number of intended experimental runs. Remember to account for the deadvolume in each bottle: 15 mL for 250 mL bottles, $3 0 ~ \\mathsf { m L }$ for $5 0 0 ~ \\mathrm { m L }$ bottles, and $6 0 ~ \\mathsf { m L }$ for $1 0 0 0 ~ \\mathsf { m L }$ bottles. Fill the alcohol (EtOH) bottle with a minimum volume of $1 0 0 ~ \\mathsf { m L }$ for the Flush and Rinse. Fill wash station Source A and Source B carboys. 3. Make sure a used, undamaged PatchPlate and a new CellPettor pipette are in place. Run the Start of Day Flush and Rinse procedure to eliminate alcohol (EtOH) from the system and prime with saline solutions. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/9ac62ca3f1097244cbeef29f6e5f98a5d62091be16d8a2a1bf4d008031d113bd.jpg) 4. Load the required labware onto the process deck. Load a clean buffer boat at the external buffer station and fill it with 25 mL to 125 mL of external buffer. Load a new PatchPlate at the patch plate station. Load a clean cell boat at the cell boat station. If you are using the CellPettor, make sure you are using a Molecular Devices standard cell boat. If applicable, load the tip rack station with a tray of disposable pipettor tips. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/54416694517c28d029234d216f238c121f0e1ab57524560df3adfb80c104ad2e.jpg) # Prepare Compounds and Cells 1. Prepare a compound plate and load it at the applicable compound station. 2. Prepare the cell suspension, resuspend the cells in a $1 5 ~ \\mathsf { m L }$ conical tube, and load the tube into the cell tube holder. If you are not using the CellPettor, manually pipette the cell suspension to the cell boat. # Select Setup, Channel, and Cleanup Protocols Choose a Setup Protocol, a Channel Protocol, and a Cleanup Protocol from the list of saved protocols. The Setup Protocol performs cell preparation steps including plate setup, vacuum checks, electrical checks, plenum filling, hole test, seal test, and perforation of the cell membrane. The purpose of this protocol is to select the PatchPlate type, run a hole test and a seal test, and obtain cell access before the Channel Protocol starts. The Channel Protocol performs the compound addition, voltage application, and data acquisition steps in the experiment. The purpose of this protocol is to measure ionic currents from the cells in the PatchPlate. The Cleanup Protocol performs wash and draining operations after an experiment is complete. The purpose of this protocol is to ensure that all instrument components are clean and ready for the next experiment. Edit parameters for each protocol, if applicable. If you are developing a new protocol, select Assay Development Mode to pause the experiment after the Channel protocol runs. # Run the Experiment ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/58ff48e01f0987a04701d976d3b0f00746e25f907609cdf5a6bfc09096e4e0aa.jpg) Screening Mode: If you are running an experiment in Screening Mode, all three protocols run without interruption. As the data is acquired, review results in the Data Acquisition and Review workspace for the hole test, the seal test, and the progressive results of the voltage-gated or ligand-gated experiment scans. Assay Development Mode: If you are running an experiment in Assay Development Mode, the instrument pauses after the Channel Protocol. At the pause, edit the Channel Protocol parameters, or load other Channel Protocols, and then run the new protocol. Continue to edit the parameters and run iterations of the Channel Protocol until you have an optimized assay. Rename and save the Channel Protocol to use later in Screening Mode. To complete the experiment, click the option to run the Cleanup Protocol. # Analyze the Data After Acquisition ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/a66313703c2bcc7ac4673f1f563bde801f96e5e52467140135f70720ef91f4ea.jpg) After the experiment completes and the data is acquired, select the Data Analysis workspace. Select the experiment data to review. Zoom any well trace to inspect results more closely. Edit and apply metrics to reduce data to numeric values. Apply filters to remove the less useful data from the results. Apply summary criteria to refine hit data. # Export Data ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/3ddf8f349074e0ac38262a7e4ccb3a274d1f16c2968bd50eaa484756e51bc7c1.jpg) Open the Metric Export dialog to select the scans and User Metrics to include in the exported data. Name the exported data file and set the folder location for data export. # Cleanup ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/d7466f39f70b6b98f50a8fd358ab70f3590095b1736f387cc4b6d5eaa12f73bd.jpg) At the end of the day: 1. Remove all labware from the instrument except the PatchPlate and the CellPettor pipette. Provided it is in good condition with no damage, the used PatchPlate can remain in place for the Flush and Rinse procedure. 2. Run the End-of-Day Flush and Rinse procedure to flush and clean the instrument with alcohol (EtOH). 3. Turn the system off. ## PM and IQOQ Services # Preventive Maintenance (PM) Compliance Assurance Digital Installation Qualification (IQ) Operational Qualification (OQ) Services PM Service confirms that your Molecular Devices instrument meets operational specifications. # Service Description A factory trained and qualified field service engineer (FSE) performs multi-point inspection procedure on your instrument to verify the instrument meets operational specifications. The rigor of the multi-point inspection procedure allows for proactive identification of replacement parts (as needed) to ensure your instrument is maintained at peak operational performance. At the completion of the PM service, the FSE presents a PM checklist which documents the completion of the multi-point inspection procedure. IQOQ and PMOQ Services certify that your Molecular Devices instrument meets operational specifications. # Service Description The IQ section verifies and documents that all necessary components required for operation are received and properly installed in accordance with Molecular Devices specifications. The OQ sections tests the mechanical, electrical, and optical components of each instrument to verify proper operating functionality in accordance with Molecular Devices specifications. The IQOQ service is performed for new instrument installations or for instrument relocations. The PMOQ service is performed on instruments that have been installed for six months or longer and includes preventive maintenance. A factory trained and qualified FSE performs operational verification procedure on your instrument, and runs proprietary verification scripts to certify your instrument meets original manufacturer specifications. At the completion of the qualification services, the FSE presents a document which includes specifications testing results and certifies that your Molecular Devices instrument meets original manufacturer operational specifications. | | | | | --- | --- | --- | | | PM Service | PMOQ/IQOQ Service | | Documentation | PM Checklist | Comprehensive digital orprinted report, including qualification results and validation certificate to ensure you are audit ready | | Execution | Factory trained and qualified FSE | Factory trained and qualified FSE | | Fulfillment | FSE confirms that customer's instrument meets operational | FSE confirms that customer's instrument meets operational | | Molecular Devices Certification | specifications No | specifications Yes | | PM Procedure | Yes | Yes + qualification results andvalidationcertificate | | Instrument Operational Procedure | Yes | Yes + qualification results and validation certificate | | Proprietary Verification Scripts | Yes | Yes+ Performance certification values | | Operational Parameter Recording | No | Yes + Performance certification values | | FSETraining Record | No | Yes | ## Organism-Specific Picking Pins # Organism-specific picking pins of the QPix 400 series ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/750ea40c6576883b708c3769d36d2dd3392c8fbc03328580f223eb0a116f7b0e.jpg) For a diverse range of high-throughput microbial workflows The QPix"400 Series of automated microbial colony pickers is designed to meet research needs across a diverse range of microbial workflows.Powerfuland versatilecolonydetectionalgorithmscombined with specializedpins are speificaly tailoredtopick,plate,andreplicate bacteria,fungi,algae,phage,andyeastcels.Pickupto3oooclonesper hourwitha typical efficiency of ${ > } 9 8 %$ , significantly increasing speed, throughput and walk-away time.The innovative, high-throughput technology platform significantlyspeeds up biologics screening by automating repetitive tasks,reducing errors and improving flexibility. # Agar height sensor and the right pin optimize colony transfer efficiency Repetitive failure of a colony transfer can result in project delays,wasted biomaterials,or the loss of valuable clones.To ensure optimal colony transfer fora diverse range of microorganisms,Molecular Devices offersa unique portfolio of picking pins that come in a variety of shapes, sizes,and textures.Moreover, a proprietary agar height sensor automatically determines the optimal picking height on-the-fly, thus enabling optimal transfer and outgrowth of biological materials.Together, proper pin selection and the agar height sensor enhance microbial colony transfer effciency by as much as $40 %$ as shown in the example dataset in Figure 1. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/113bba5e26f18cd9d6be1e267a39a586e5bd9a48ce57efcd97f17c9e7650eb6d.jpg) Figure1.Colonytransferefficienciescanvarywidelyaccordingto thepairings betweenmicroorganisms and colony-picking pins.A representativedatasetisshown forE.coli,Saccaromycescaervisiae, and S.venezuelae. # Pin features · Range of organism-specific pins · Extends 8 proprietary spreading pins simultaneously · Five pin shapes for picking loaded into fully automated pneumatic, 96-pin picking head · Customer-interchangeable heads for otherapplications · Automatically sterilized between runs and reusable · Proper pin selection and agar height sensor enhance microbial colony transfer efficiency # Pick colonies with a typical efficiency of >98% ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/8ceddd3015dfcf3830275f78b9b0ebbb5c0c892c07aeaad93822a96bc6493431.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/28652517b06e572505b114b5f93fdbc8dbc20fc9a68d7cefee6da642f09a76df.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/ff79ae2e83925915532c8e98f4b2fcd0d32a0753f59063587c784f2ef6c10281.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/8a466514f71238d266828adef0ca5568ecb17be3440ff80ca9c47aada32b9947.jpg) Sensors detect agar height, helping high-precision robotics pick single colonies gently. Organism-specific pin heads are tailor-made to ensure maximum transfer of material Proven pin sterilization process is suitable forany organism. Barcode reader tracks sample plates,wells and picked clones forperfect record keeping. # Reutilize picking pins with the built-in washing bath Many colony pickers rely on either pipette tips or a feed of polymer reels to pick microbial communities. Not only does the QPix 40o series employ a range of different pins,but the built-in washing system means these pins can be indefinitely reused.The three-bath system,typically filled with bleach, sterile water and ethanol,also includes a halogen lamp.This system ensures the pins are washed, disinfected,dried and ready to reuse in a matter of minutes.Programming flexibility also allows complete customization of the washing technique to suit individual research needs. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/4f0f98007f35d5e6ea46a62b83fa96af62e564a645339dd06a013e6908ddf5b1.jpg) # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. # Regional Offices USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Belgiu,rk,d,re,ermyd,thrs,indd ## High-Throughput Stem Cell Screening ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/78453780af61b3dda13bb57f3c099d3f763460d02c6ed81be19011d585a74ff2.jpg) # High-throughput stem cell screening Regenerative medicine has the potentialto provide cures forsome of the world's most devastating diseases.However, many roadblocks remain when it comes to moving these therapies from the benchto the bedside.A major remaining roadblockis thelaborious nature ofmanually screening through large populations ofcels.Automating this process allows agreater numberofcoloniestobe screened-increasing boththenumberof coloniesabletobescreened,aswellas time spentonmore technical parts ofthe discovery processThe abiltyto adapt high-throughput screening technologies to stemcell workflows willallowscientists toaccelerate theirdiscovery processesand,inturnmove theirnovel therapeutics down the pipeline more quickly. Here,we highlight a general workflow and systems for increasing the throughput of stem celland regenerative medicine workflows. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/1f0fd3fc7a8302820617fbe697ae8ef11dbe97f0a85285fd64a107f75fb264be.jpg) # Colony formation Individual stem cells plated at a low density in 6-well plates allows them to divide and develop into colonies.Plating density is kept low to ensure that colonies are derived from a single parent cell. # 2 Clone screening Clonally-derived stem cell coloniesare identified by their desirable morphological characteristicsand are picked from the low density 6-well plates into higher density 96-well plates to carry forward with the screening.The ClonePix@ 2 Mammalian Colony Picker can be customized with high resolution optics and stem cell specific pins,allowing this established technology to be leveraged in stem cell workflows. # 3 Cell growth Cell growth is determined by monitoring cell division over a given period of time using label-free imaging. # 4 Functional screening In addition to monitoring growth, functional cell based assays can be performed.This can include assays assessing differentiation potential, pluripotency,ability to form 3D organoids,and other desirable traits. # Technology highlight ClonePix system with stem cell picking # Screen more clones in less time ClonePix 2 system customized for stem cell picking is faster and more efficient than labor-intensive limiting dilution and FACS. Our sophisticated software and integrated robotics enable a picking speed of $>$ 10,000 clones per day. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/4aaa5ff740f22f5ca598cd64a524460ff03b782b98e13483d226880d3d0fd3eb.jpg) # Selectcells with desirable attributes Easily screen and select clones based on cell morphology, cell viability, orany marker that is required in yourworkflow. # Pick colonies with accuracy Robotic picking reduces risk of colony disturbance.Images of picked clones are stored with data. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/f3aa391ca5e27a8a3ce9612ffec9ba7f5a3d101bac7acf5ef35fd648fddb6aba.jpg) # To learn more, please contact your regional Molecular Devices technical sales specialist, visit [www.moleculardevices.com](http://www.moleculardevices.com/) or call +1.877.589.2214. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Belgrk,d,re,ryed,thsindd ## PatchPlate Handling Guide # Premium PatchPlate Handling Instructions The Premium PatchPlate™ is available for single-use in the IonWorks Barracuda™ Automated Patch Clamp System for ion channel measurements. # Storage Precautions Keep the Premium PatchPlate frozen until ready to use. Keep the Premium PatchPlate in the factory packaging until ready to use. Keep the Premium PatchPlate in a vibration-free, and shock-free location. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/35e8cbdd955268a4c2a039258cf8f3ad85dd168ad5b063120889573fba73b225.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/d83c4dd4f92b24ad8b7e38f514694b38122884279b6c0d14bd56e7c64754e971.jpg) # Use Precautions Do not use the Premium PatchPlate if it was dropped during handling because it may be damaged. Do not use the Premium PatchPlate if the low temperature cryopreserving causes plate brittleness and deterioration. Dispose of the used Premium PatchPlate according to your company’s disposal protocol. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/32c51f49f9d626af4b45f3ea40ae0d49c93ef21654b6f686a77e04d467efbe07.jpg) # Storage Temperature and Shelf Life | | | | --- | --- | | Item | Specifications | | Storage Temperature | -70°C or colder | | Shelf Life | 3 Months or less from the date on the package. | | Note: store in factory packaging in \_a vibration-free and shock-free location. | | OperatingTemperature | Humidity:90% RH max. No condensation Room temperature +20°C to +25C | # Thaw Protocol Take the package out of the freezer and leave it in the unopened packaging for 5 to 10 minutes until it reaches operating temperature. After the Premium PatchPlate reaches room temperature, remove it from the packaging, place it in the instrument, and immediately start the measurement. CAUTION! Never touch the Premium PatchPlate with bare hands. # For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC. Patent Pending. Product manufactured by Molecular Devices, LLC. 1311 Orleans Drive, Sunnyvale, California, United States of America 94089. Molecular Devices, LLC is ISO 9001 registered. $\\circledcirc$ 2011 Molecular Devices, LLC. All rights reserved. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/dd5a56b2a59af7cc3cf22a6cd932a09208a963f46c489da985cdd29cde0faaa3.jpg) ## REGE Installation Guide # lonWorks Barracuda? Replaceable Ground Electrodes (REGE) Installationand Removal Quick Reference Guide This guide details the following Replaceable Ground Electrodes (REGEs) Plenum maintenance procedures (Figure 1-1): Installing REGEs in the Plenum on page 1 Removing REGEs from the Plenum on page 4 For instrument maintenance purposes,Molecular Devices recommends removing and conditioning each installed REGE set nightly. Referto your lonWorks Barracuda Automated Patch Clamp System User Guide for additional information and procedures. # Installing REGEs in the Plenum Only install REGE sets that have been properly conditioned. Refer to your lonWorks BarracudaAutomated Patch Clamp System UserGuide forprocedures. CAUTION! Always wear gloves when working within the lonWorks Barracuda instrument and handling the REGE parts.Never touch the top surface of the ground electrodes with your bare hands,because it results ina build-up of oils on the electrodes that impairs the functionality. To install the REGEs in the plenum: 1.Verify that the O-ring and spring are correctly positioned,and correct as needed. The O-ring should be seated in the designated O-ring counter-bore,and the spring should be over the plug in the center of the socket with the narrow diameter of the spring at the top (Figure 1-1, Figure 1-2). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/507cee8dcdc8c45a3c0aace25f079004c4afd3386c4af3bac55132271b8dcc45.jpg) Figure 1-1:Correctly Positioned O-Ring and Spring in Empty Plenum Socket ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/f39022ecaf4eb641abb24b7cc76b748d422df4c2dbfa76a1c032727890332b4a.jpg) Figure 1-2: Incorrectly Positioned O-Ring in Empty Plenum Socket 2Verify that the plenum socket is dry. Depending on the amount of liquid in the socket,use a cotton swab or wipe cloth (Figure 1-3). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/9aba2d1d3cd63aabf72dea3fddb64b447617ec0ecd30ddc8d9b84afb66d6c4df.jpg) Figure 1-3:REGE Plenum Socket Drying 3.Wet the REGE housing screw threads with $1 0 \\mu \\mu \\up$ to $2 0 \\mu \\mu \\mu$ distilled (Dl) water for lubrication.The amount of Dl water depends on how long your REGE set has been dry. If you are installing immediately after conditioning,when drying the housing, leave $2 0 \\mu \\mu \\up$ of the Dl water rinse on the threads. If you are installing when the REGE set is completely dry, lubricate the housing threads with $1 0 \\mu \\mu \\up$ of Dl water, rotate the housing180 degrees,and apply $1 \\bar { 0 } \\mu \\mu \|$ of Dl water to the threads again (Figure 1-4). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/6d3a70d9a8485dba04f95e78c57926a93da3a39255b53a84f4aa63f598000b81.jpg) Figure 1-4:REGE Housing Threads Lubrication with $2 0 \\mu \\mu \\mu$ Distilled Water 4.Insert the lubricated REGE loosely into an empty plenum electrode socket (Figure 1-5). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/f7fefad83fc0b2b9c5a756ff1e4757eb8d3a30b3339e6ae26afbdc23ade3dc0a.jpg) Figure 1-5:REGE Placement ina Plenum Socket Note:Try not to dislodge the spring and O-ring inside the plenum socket. If the spring and O-ring inside the socket become dislodged,put them back in place. 5.Using the provided REGE service tool,seat the tool prongs into the holes in the electrode housing for installation (Figure 1-6). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/7b6699464a98a0cce1b038dd85a7810fb8f9abe89eef89b25cde3b297ddaecca.jpg) Figure 1-6:REGE Service Tool Prongs Alignwith Holes in the Electrode Housing 6. Turn counterclockwise a /4 turn to align the male and female threads and reduce the risk of cross-threading. 7.Place your index finger on one hand on the magnet on the top of the service tool and apply downward pressure while using your other hand to turn the service tool clockwise to keep the service tool pins in the electrode housing service tool holes (Figure 1-7). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/7c7617c20d8cead7051b6a0e687b441798bbbeda67ba7de16b121b6d3619753b.jpg) Figure1-7:Two Hand Usage of the REGE Service Tool 8.Turn until the electrode housing seats in the plenum socket flush with the plenum top. CAUTION! Avoid over tightening the REGEs.Excessive tightening force can break the REGE housing and can crack the plenum. 9.Run a gloved finger along the seam between the REGE and plenum to confirm a flush installation. The two surfaces should be smooth and bump-free (Figure 1-8, Figure 1-9,and Figure 1-10). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/a00cb6c28b8c5d4679fd3447cebfd7d9d23fa405f4bb4bebbbdbe12c68b9f6e4.jpg) Figure 1-8:Gloved Finger Flush Installation Test ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/ef0780f7514b92746b68b10a84656283c39ee08867dac615ddb4ef310b9d82d0.jpg) Figure 1-9:Correct Flush REGE Installation ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/93ff1628b80c053dc61022337e5f67e40920a7733faee676af8092d5af0950c6.jpg) Figure 1-1O: Incorrect Raised REGE Installation 10. Repeat steps 1-7 until all four new ground electrodes have been added to the plenum. 11.After allfour REGEs are properly installed in the plenum,you can begin your assays. CAUTION! Immediately before you start your assays,when the REGEs are dry, make sure no other fluid touches the REGEs.Dry REGEs rapidly absorb approximately $2 0 0 ~ \\mu \\iota$ of the liquid that first wets them,and it takes about 90 minutes for the REGEs to equilibrate to a different liquid. # Removing REGEs from the Plenum Remove and condition each REGE set nightly after use.You reuse the REGEs that you remove,so remove them carefully.Always wear gloves when working on the lonWorks Barracuda instrument and handling the REGE parts. CAUTION! Never touch the ground electrode top surface with your bare hands,because it results in a build-up of oils on the electrodes that impairs the functionality. # To remove REGEs: 1. Using the provided REGE service tool, seat the tool prongs into the holes in the sides of the electrode housing for removal (Figure 1-6 on page 2). 2. To keep the service tool pins in the electrode housing service tool holes,place your index finger on one hand on the magnet on the top of the service tool,and apply downward pressure while using your other hand to turn the service tool counterclockwise until the REGE housing unscrews and is loose in the plenum socket (Figure 1-7 on page 2). 3.Lift the REGE housing out of the plenum socket and carefully set it aside for conditioning (Figure 1-5 on page 2). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/f7978b53c7398d2d0447721c51b896448300cc9d340406a0da6e15bbd2999dc2.jpg) Note:Try not to dislodge the spring and O-ring inside the plenum socket. If the spring and O-ring inside the socket become dislodged,put them back in place. 4.Dry the plenum socket (Figure 1-3 on page 1). 5.Repeat steps 1-4 until allfour REGEs have been removed from the four plenum sockets. 6.Condition all four REGEs to continue using them in the lonWorks Barracuda system.Referto lonWorks Barracuda Automated Patch Clamp System User Guide for procedures.. Note: Proper conditioning takes up to 15 hours. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/ebfe62e9504c60413d11b3ad139fc8da00fb55a1ba55355673d1de64fc270fb7.jpg) 7.To continue using the lonWorks Barracuda system while the recently removed REGE set conditions over night, install the provided second REGE set. See Installing REGEs in the Plenum on page 1,otherwise,place a written note over the plenum that reads something like “Electrodes Have Been Removed"as a precautionary reminder to keep the plenum dry. For research use only. Not for use in diagnostic procedures. ThetrademarksmentionedhereinarethepropertyofMolecularDvices,LCortheirespectiveowners.hesetrademarksmayote used in any type of promotion oradvertising without the prior written permission of Molecular Devices, LLC. # Patents: [http://www.moleculardevices.com/productpatents](http://www.moleculardevices.com/productpatents) ## Streamline Synthetic Biology ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/817fad43295541805009803058b3d941e976058d5a0736d899f2d76fbcf5529e.jpg) # Streamline your synthetic biology workflow with solutions from Beckman Coulter Life Sciences and Molecular Devices Synthetic biology is an interdisciplinary science with the potential to impact academic and industrial applications including the creation of novel therapeutics and vaccines, plant science and biofuels. The focus is often on generating, characterizing, and isolating parts of natural biological systems before using them as components of an engineered biological pathway. A trademark of synthetic biology is the application of rational principles to the design and assembly of these biological components. Even with rational design, the impact of introducing foreign DNA into a cell can be difficult to predict. This creates the need to test multiple permutations to obtain the desired outcome. An emphasis on the modular design of DNA parts enables the assembly of a greater variety of potential constructs by interchanging the individual components. Automation of the assembly process reduces the time, labor, and cost of generating multiple constructs to allow for an increase of throughput with an overall shortened development cycle. Double-stranded DNA fragments are designed for easy gene construction. The assembled constructs are typically cloned into an expression vector and verified with colony qPCR or Next-Generation Sequencing (NGS). However, in some high-throughput workflows this verification step can be optional. The synthetic constructs are then analyzed in variety of functional assays. After learning from the assay results, the constructs can then be modified or refined. This design, build, test, learn cycle is repeated until a DNA construct is obtained that produces the desired function. # Key benefits • The solutions offered by Beckman Coulter Life Sciences and Molecular Devices, both part of Danaher Corporation, are essential in automating the main steps of these high-throughput synthetic biology workflows. • Reduced reaction volumes offer significant reagent cost and time-savings while improving reaction efficiency. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/4e17098f3127fb0e39a2d55219e88dd936e84fb6cac915d555271580bd8842b1.jpg) Gene assembly workflow # Design process The in-silico tools to design the high-throughput gene assembly experiments. # 2 Ordering gene fragments and vectors Gene fragments and vectors from a variety of vendors, such as Integrated DNA Technologies (IDT), can be directly ordered in Echo source plates at the ready-to-use concentrations. # 3 Fragment pooling Echo acoustic liquid handling technology enables highly accurate, fully automated, non-contact dispensing of fluids in nL to μL volumes. By reducing reaction volumes the Echo offers significant reagent cost and time savings while improving reaction efficiency. # 4 Gene assembly A thermocycler or heating block for heating of the reaction mixtures and formation of the assemblies. # 5 Transformation The assembled genes are transformed into competent cells. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/7fd2f25068398d4c8bd737a7bc92b240f395395f16dc494c747b1c97fab97ba5.jpg) # 6 Colony arraying Echo acoustic liquid handling technology enables high-density and high-throughput fully automated colony arraying onto media/ agar plates. Hundreds of Petri dishes can be replaced with one densely spotted plate (up to 1536 spots). # 7 Colony selection Automated colony picking allows scientists to focus on discovery, instead of performing repetitive tasks. The QPix microbial picker uses precision robotics to quickly and accurately pick 3,000 colonies per hour. # 8 Plasmid prep Automated plasmid prep on Biomek workstations reduces hands-on time and pipetting errors associated with manual processing. Ready-to-implement demonstrated methods provide quick implementation. # 9 QC (optional) and functional testing An Echo or Biomek i-Series can be used for setting up an optional QC step to select for successful gene assemblies. A variety of methods, such as NGS or colony qPCR can be used for the QC step; or instead a functional assay will be immediately pursued to select for the successful clones. # 10 Learning The learning from the round of experiments will be applied to the future in-silico design steps. A 1536 pattern of bacterial colonies being arrayed by the Echo 525. Image credit: George McArthur, Ansa Biotechnologies # BECKMAN # Life Sciences ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/c677f42d9a6391d234d6fd5e002b183169dc487daed2c0813a090a9036e98b0c.jpg) # Echo 525/650 Series Liquid Handlers Up to $\\sim 7 5 %$ faster gene assemblies and up to $\\sim 8 5 %$ more efficient assemblies at reduced volumes, to reduce assembly cost by up to $\\sim 9 8$ Reduced cost of QC (up to $90 %$ ) using colony qPCR/NGS due to accurate and precise low-volume reaction setup Up to $9 7 %$ faster normalization and pooling of NGS libraries Automated system for high-throughput and high-density colony arraying (up to 1536 colonies per plate) • Consistent cell transfer ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/fc03217932155c72f7149c5e667ac75456507f70f984d58348fa9e2c9abeb114.jpg) # MOLECULAR DEVICES # QPix™ Microbial Colony Picking Series • Fully automate synthetic biology workflows for DNA assembly, antibody discovery and protein engineering with library management • Streamline your workflow with scalable automation – pick up to 30,000 colonies per day • Automate several sample prep and plate handling processes such as transfer of bacterial liquid culture and plating on agar • Electronic data tracking for well-documented data control # BECKMAN COULTER # Life Sciences ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/92a0ce95aef5b7bef3ed828d590dbae947342f51fcd4707c89f3bc3634a9bd75.jpg) # Biomek i-Series Automated Workstations • $0 . 5 \\mu \\mathrm { L } - 5 \ m L$ pipetting capability to automate a wide range of workflows including extraction, plasmid-prep, NGS library prep, cell culture and functional assays • On-deck devices (e.g. orbital shakers, peltiers) and integrated devices (e.g. plate washers, incubators) to control sample processing and minimize hands-on time • Track sample and plate data with DART (Data Acquisition and Reporting Tool) • Mid-throughput (i5) and high-throughput (i7) workstations to suit your workflow needs ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/a9156623c08bb8bfb3314497413abb9cfe97957d440ec9d51a856a18125e2409.jpg) # Life Sciences # CosMCPrep Plasmid Purification Kit • SPRI paramagnetic bead-based purification system to purify a variety of high and low copy number template types # Beckman Coulter Life Sciences Customer Breakthrough “The Echo 525 automates complex liquid aliquot rearray at high speeds and at miniaturized scales, saving significant time and money. It has proven to be extremely versatile and robust; my teams have used it to set up PCRs, cell-free protein synthesis reactions and biochemical assays, and even to array colonies of transformed bacteria on solid media. At Ansa, the Echo 525 is the workhorse for our automated DNA assembly platform.” —George McArthur, PhD, Head of Product, Ansa Biotechnologies ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/76e8eb1b017e46a15b0dc4f1057e2b15f7acd60374d15fd14728e216dc52a7d8.jpg) # Molecular Devices Customer Breakthrough Zymergen uses the QPix colony pickers to make better microbes in industrial fermentation. Zymergen’s growing fleet of Molecular Devices QPix™ 420 Systems allows them to identify and pick large numbers of colonies quickly, accurately, and reliably. The QPix units are a critical link in their microbe engineering pipeline, integrating seamlessly with their state-of-the-art laboratory information management system (LIMS). To find out more about the systems, contact your Beckman Coulter Life Sciences, IDT or Molecular Devices sales team or use the contact information below. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/d5376abe3b5e6898718232e0668d1818c9950dbfb1a5e170921f1d31bd81732e.jpg) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ## Phage Display Screening Innovation # Advances in phage display screening with technology innovation # Overview Phage display was first described in 1985,when George P. Smith demonstrated a way to insert an exogenous gene for a protein of interest into a bacteriophage coat protein gene.This caused the phage virus to display the protein on the phage surface,resulting ina connection between genotype and phenotype.The importance of phage display was recognized in 2o18,when half of the Nobel Prize in Chemistry was shared between George P. Smith and Gregory P.Winter for their work developing the technique.Phage display was used to develop Humira (adalimumab), first approved in 2oo2,and countless other biological therapeutics. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/67fc889d59a826454027446c0b8a67c95e7eb3be518cdddb3d6ac64fe0cd6b9b.jpg) More and more industrial applications from biotech to synthetic biology are using phage display because of its high throughput capabilities for protein interaction determinationand protein engineering.However, in order to select high-affinity binders,or ligands,which can recognize a naive target froma phage library $( ^ { \\sim } 1 0 ^ { 7 } – 1 0 ^ { 1 2 } )$ non-binding bacteriophages need to be washed away, and phages that bind specifically with target molecules are eluted and harvested through 3-5 rounds of panning. Using conventional methods,this can be laborious to perform when screening multiple selections with different antigens simultaneously. To speed up their screening, scientists are incorporating new technologies like the ${ \\mathsf { Q P i x } } ^ { \\mathsf { \\pi } }$ Microbial Colony Picking System into their workflow to speed up their phage display screening (Figure 1). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/61b3c8971e15ff4d6a1c9d4026036725ff579ade6ebe368417d7fe0f1454cd2b.jpg) Figure1.Typical phage display workflow showcasing where the QPix systemcan be used. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/094b269986a8f9659554e07a8fc206cc0e5120a6c8edfca6d02403ff1aabf236.jpg) # QPix system workflow Pistette # The QPix 400 Series The QPix 40o Series of Microbial Colony Pickers alow youto simultaneously detect colonies and quantifyfluorescent markers when pre-screening colonies before picking.Objective,quantitative identification combined with high accuracy picking ensures therightcolonyis picked every time.This automated microbial screen systemcan pick upto3000 clones per hour with a typical efficiency of ${ > } 9 8 %$ ,significantly increasing speed, throughput and walk-away time. Additionally, clones can be screened in whitelightorfluorescence,and the systemcanselectclones based on user-defined parameters suchascompactness,axis ratio,size,proximityand fluorescence level(Figure 2).This innovative high-throughput technology platform significantlyspeeds up phage display screening byautomating repetitivetasks,reducing errors, and improving flexibility. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/f79617d172327c93839cdab83abbfc692000f794332f6c672e0790494496b2da.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/91760ea3b8127da53a6de5aa8b8f80d426e1308638ed46b4dc74a4eb18ec3f6d.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/bpd/images/2aa330a8004a640099ec66b02616e547c40836805cbfa8ada891bf7ccb0487dd.jpg) # Image analysis software identifies individual colonies in white light. Colonies selected according to user-defined parameters: compactness, axis ratio,size,and proximity. # Optional pre-screening provides unique information to identify the best colonies. Colonies selectedaccording to user-defined parameters:compactness,axis ratio, size,proximity,and fluorescent level. # Predefinednumberofcolonies selected per region for picking. Yellow:Colonies to be picked based on selection criteria. Green: Desired number of colonies met.Red:Desired number ofcoloniesnotmet. Figure2.TheQPixsystemssimultaneouslydetectcoloniesandquantifyfluorescentmarkerswhenprescreeningcoloniesbeforepicking # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing ofworldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,eli,k,dre,yd,thsindd ## REGE Kit Conditioning Guide # lonWorks Barracuda? Replaceable Ground Electrodes (REGE) Kit Conditioning Guide This kit is for use with the replaceable ground electrodes (REGEs) plenum for your lonWorks Barracuda?Automated Patch Clamp System. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/b7e9ca54ee5ec90285a1273133e6522858525549a42090d4c6a3408fa75f2abb.jpg) Figure 1-1:The PatchPlate Station Replaceable Ground Electrodes Plenum | | | | --- | --- | | Position Component | | 1 | Replaceable ground electrodes | | 2 | Amplifier touch pads | | 3 | Plenum O-ring (rubber gasket) | Each REGE set is serialized.On the bottom of each housing within a set is a serial number and a letter (Figure 1-2). Each set should have the same serial number along with the letter A,B, C,or D so you can track each REGE location within the plenum slots,as needed.Molecular Devices recommends using the REGEs as complete sets. Avoid mixing multiple sets. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/2acb27c1aac2a6559991bae4227e8ea0800a3f473ee174b2114a14c63a0cbef9.jpg) Figure1-2: REGE Set Serialization For instrument maintenance purposes,Molecular Devices recommends removing and conditioning each installed REGE set nightly. For proper usage and maintenance,this guide provides the process for Conditioning a REGE Set. Refer to your lonWorks Barracuda Automated Patch Clamp System User Guide for additional information and procedures. # Conditioning a REGE Set The purpose of the conditioning process is to equilibrate the REGE set to the internal buffer that you will use in your next assay. Before installation and use,the REGE set must be conditioned in the correct internal buffer. For ideal equipment maintenance purposes,Molecular Devices recommends also conditioning each REGE set at the end of the usage day using fresh internal buffer overnight. After conditioning,the REGEs go back in to the lonWorks Barracuda PatchPlate station REGE plenum sockets to continue running assays,or should be stored dry in the original packaging. REGEs stay conditioned as long as they are unused, untouched by bare hands,and properlyequilibrated. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/dbb1b5bf55791d75a35dc54e0b47b0e1a0106037de8b47d45307da412057eab2.jpg) CAUTloN! Never touch the top surface of the ground electrodes with your bare hands,because it results in a build-up of oils on the electrodes that impairs the functionality. To condition a REGE set: 1. Fill the included deep buffer boat with the same kind of internal buffer that you will use for your next assay. 2. Place the four REGEs upsidedown in the buffer boatwith the electrode heads on the bottom of the buffer boat. 3. Add enough additional internal buffer fluid,as needed,until the electrodes are completely submerged (Figure 1-3). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/3c270359411878b3869c85b8cadce3be6e5c6810a2bd99622a6ee7e7ad6d9283.jpg) Figure 1-3: REGEs Soaking in Condition Solution 4.Remove air bubbles trapped against the electrodes by gently tapping the submerged housings. 5.Soak overnight.Molecular Devices recommends between 13 to 15 hours. 6.After soaking the electrodes overnight,wearing gloves,remove the REGEs from the buffer boat,and rinse each briefly over a sink with distilled water. 7.Dry the REGE housing,especially the underside (Figure 1-4). ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/afcce5c1c31d6e9dcc9e619e9e3176b7ae680f4114704fb97cae3ca8eaa3085b.jpg) CAUTIoN！ If not removed from the underside of the REGE housing, the salt in the internal bufferaccumulates in the REGE plenum socket,which can cause an open circuit when the salt dries. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/dd/cns/images/18b4e1041ee531f6ed7fba48970c1eee9ad31f4035488744215cbe16868d954e.jpg) Figure 1-4: REGE Housing Underside Drying 8.Install the REGEs in the lonWorks Barracuda instrument plenum to continue runningassays.Refer to your lonWorks Barracuda Automated Patch Clamp System User Guide for installationdetails. 9\. Afterconditioning,unused REGEs should be stored dry in the original packaging with the lid closed. CAUTIoN! Once your REGEs are dry, if you are going to immediately use them to run assays,do not rinse the electrode heads with distilled water,because your assays can experience voltage drift while the chloride concentration in the electrodes equilibrates to the chloride in the internal buffer. When completely dry, the REGEs rapidly absorb approximately $2 0 0 ~ \\mu \\iota$ of the liquid that first wets them,and it takes about 9O minutes for the REGEs to equilibrate to a different buffer. ## Colony Picking Solutions Build more, screen more, increase your colony picking capabilities 10x! Scale is becoming an increasingly important factor in biology. The ability to scale up a process increases the chances that one is able to more readily and efficiently identify better hits earlier in the process. Library screening is a critical step in many workflows, ranging from synthetic biology to CRISPR and gene editing. With improvements in library synthesis, it is now possible to easily and inexpensively manufacture extremely large and diverse genetic libraries. The increased quality of these genetic libraries, in theory, yields more ideal variants as researchers are able to begin with access to a larger starting pool of possibilities. The ability to manufacture and easily engineer large libraries introduce a new bottleneck into the process— traditional colony screening and picking is slow, laborious, and time-consuming. This leaves groups with two choices: hire additional staff for mindless colony picking sessions, or turn to proven technologies to increase the accuracy and efficiency of repetitive tasks, freeing scientists to analyze data. The QPix™ Microbial Colony Picker leverages best-in-class colony picking technology to alleviate this bottleneck to quickly, accurately, and efficiently screen through massive genetic libraries. The easy-to-use, intuitive software guides users through setting up colony picking runs where precision robotics pick the right colonies every time. Data is automatically recorded into the machine’s database, providing users with a complete audit trail and sample tracking, ensuring that no data is ever lost. Our modular, scalable series of colony pickers allows groups of all sizes to increase the accuracy and throughput of their workflow, while still allowing for future throughput growth. 0 500 1000 1500 2000 2500 3000 Throughput QPix Select HT QPix 420 Manual Labor distribution ■ Walkaway time ■ Hands-on time Colonies/hour Minutes QPix Select HT QPix 420 Manual QPix Select HT QPix 420 Manual Walkaway time Colonies/hour 0 500 1000 1500 2000 2500 3000 0 10 20 30 40 50 60 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ©2021 Molecular Devices, LLC 1/21 2374A Printed in USA Phone: +1.800.635.5577 Web: www.moleculardevices.com Email: info@moldev.com Check our website for a current listing of worldwide distributors. \\* Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland Regional OfficesContact Us USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.3971.3530 To learn more, please contact your regional Molecular Devices technical sales specialist, visit www.moleculardevices.com or call +1.800.635.5577. Throughput 3,000 colonies/hour QPix 420 system The QPix 420 system is a great entry level unit for groups looking to increase the throughput and efficiency of their colony screening. With the ability to pick up to 12 96-well plates before needing manual intervention, this will increase your walkaway time greatly. Additional plating and streaking and library management features with complete audit trail and sample tracking allows it to become an entire library management system—taking the guesswork out of sample tracking. Hands-on time 90 seconds to set up Walkaway time 25 minutes at a time – only return to swap destination plates after 12 are full Throughput 3,000 colonies/hour QPix Select HT system For groups looking to maximize the throughput and efficiency of their colony screening and picking process, the QPix Select HT system combines the precision and accuracy of the QPix colony picker with state-of-the-art laboratory integration that can be run 24/7 without manual intervention or supervision. Hands-on time 90 seconds to set up Walkaway time Entire duration of run Manual picking Highly subjective and prone to human error, manual picking is a slow, laborious, and time-consuming process. Throughput 300 colonies/hour Hands-on time 60 minutes Walkaway time 0 minutes ## SoftMax Pro GxP Compliance ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/b757373bad913f5c0ad8a22e09c0a28941265d415f1436738d8b61ac84b97551.jpg) # Become compliant with SoftMax Pro GxP Software SoftMax Pro $\\mathsf { G x P }$ Software is the latest, most secure software to achieve fullFDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflowsto ensure data integrity.Every step is optimized to simplify analysis and reporting to support ourmicroplate readers. Below are key guidelines that regulated labs are required to follow to become compliant. # User administration Regulatory guidelines require that only authorized individuals have access to the system.SoftMax? Pro $G \\times \\mathsf { P }$ Softwareallowsuseradministration either via the $\\mathsf { G x P }$ Admin Software,or users can connect to their company's Windows Active Directory (starting at version 7.1.1) system.This simplifies defining password criteria,reset,and change periods. $G \\times \\mathsf { P }$ Admin Software also comes with three predefined factory roles; Scientist, Lab Manager,and Lab Technician roles,to accommodate the document release workflow.Permissions are defined ona per role basis and assigned accordingly to a user within a project. Users can have different roles in different projects.Granular permission setting options areavailable to create custom roles based on your needs. # System audit trail Regulatory guidelines require the use of secure,computer-generated audit trails that record date and time stamps of end user actions.Each SoftMax Pro $\\mathsf { G x P }$ Software data document file has its own audit trail. The $\\mathsf { G x P }$ Admin Portal software maintains filterable systemaudit trail information that reports end user activities within the software and database. # eSignatures and time stamps Regulatory guidelines require that the electronic signatures be used to hold individuals accountable and responsible for actions.Electronic signatures should employ at least two distinct identification components such as an identification code and password.SoftMax Pro $G \\times \\mathsf { P }$ Software is designed where electronic signatures are linked directly to their respective electronic records and cannot be decoupled from the records itself. Electronic signature functionality requires the entry of both username and password;and both must be re-entered for each subsequent application of an electronic signature. # Validation package Regulatory guidelines require validation of the system to ensure accuracy, reliability,and consistent intended performance and the ability to discern invalid oraltered records.The Molecular Devices Professional Services team of experts provide validation services to help end users achieve FDA 21 CFR Part 11 and EudraLex Annex 11 compliance. | | | | | | | --- | --- | --- | --- | --- | | Contact Us | Regional Offices | | Phone: +1.800.635.5577 | USA and Canada +1.800.635.5577 | | Taiwan/Hong Kong +886.2.2656.7585 | | | Web: www.moleculardevices.com | United Kingdom +44.118.944.8000 | | Japan | +81.3.6362.9109 | | Email:info@moldev.com | Europe\* | 00800.665.32860 | South Korea | +82.2.3471.9531 | | Check our website for a current | China | +86.4008203586 | India | +91.73.8661.1198 | | listingofworldwidedistributors. | Austria,k | ## Upgrade SpectraMax i3x # How to upgrade your SpectraMax® i3x Multi-Mode Microplate Reader with additional detection modes | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | | Cartridge Description | Part number | Specifications | Optimized sensitivity | Guaranteed sensitivity | Slots used | | Dual Auto Injector | · SpectraMax i3x Injector Cartridge with SmartInject" Technology ·Expand your research capabilities to include flash-based applications,including dual luciferase and ATP assays. | 0200-7029 | Wavelength range (LUM): Visible to 650 nm Dead volume: <10 μL with Reverse Prime function | 20 amol ATP (“flash" luminescence using Promega Enliten Assay System) | 50 amol ATP (250 fM @ 0.2mL/well,“Flash" luminescence using Promega Enliten Assay System) 3 fmol ATP (15 pM @ 0.2mL/well,“Glow" luminescence using PerkinElmer ATPlite 1step Luminescence | 2 | | ScanLater WB | ·Western blot detection using ScanLater Western Blot Assay Kit ·TRF-based with 340/80 nm EX and 616/10 nm EM | 0200-7027 | EX range: 340/80 nm EM range: 616/10 nm | High fg levels of Streptavidin | Assay System) High fg levels of Streptavidin | 2 | | AlphaScreen | ·AlphaScreen and AlphaLISA detection using 1W 680 nm EX laser diode and a 570 nm (100) EM filter | 0200-7017 | Alpha 384 STD 96- and 384-well plates | | <100 amol (384-well) | 1 | | | ·Pick best speed,sensitivity,and price for your needs ·Guaranteed sensitivity: <1oo amol of biotinylated-LCK-P peptide, | 0200-7018 | Alpha 384 HTS 96- and 384-well plates | | <100 amol (384-well) | 1 | | 25 μL/well in a 384-well plate,using AlphaScreen Phosphotyrosine (PT66) Assay Kit | 0200-7019 | Alpha 1536 HTS 96,384,and 1536-well plates | | <100 amol (384-well) | 1 | | HTRF htrf TRF | ·Cisbio HTRF detection with optimized Xenon light source and 616,665 nm EM filters ·LED light source | 0200-7011 | 6- to 1536-well plates | Exceeds Cisbio certification requirements | 96-well: 0.1 pM | 1 2 | | TRF FP | EX:370/80 nm EM: 616/10 nm,642/10 nm ·Suitable for assays using europium and samarium chelate labels ·Fluorescence Polarization | 0200-7008 | 6- to 1536-well plates | 96-well: 0.03 pM 384-well: 0.03 pM 1536-well: 0.125 pM \*Specifications listed are for europium | 384-well: 0.1 pM 1536-well: 0.375 pM | | | | detection for fluorescein- or rhodamine-like labels,using specific LED and EX/EM filters ·6- to 1536-well plates | 0200-7009 | Fluorescein FP EX 485 nm, EM 535P and 535S nm | 96-well: 1.0 mP 384-well: 1.5 mP 1536-well: 2.0 mP | 96-well: 3 mP 384-well:4 mP 1536-well: 6 mP | 1 | | 0200-7010 | Rhodamine FP EX 535 nm, EM 595P and 595S nm | | | | # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | | Cartridge | Description | Part number | Specifications | Optimized sensitivity | Guaranteed sensitivity | Slots used | | Fluorescence Intensity (FI) FI | ·Fluorescence Intensity (Fl) detection for coumarin-, fluorescein-,or rhodamine-like labels,and FRET ·Ultra-high-power LEDs for the excitation of various | 0200-7002 | EX:360/35 nm EM1: 465/35 nm EM2: 535/25 nm | 384-well plate (75 uL): 10 fmol/well 1536-well plate (8 uL): 3 fmol/well | | 1 | | Glow Luminescence (LUM) Detection Cartridge LUM | ·Glow Luminescence (LUM) Detection | 0200-7012 | 96-,384- and 1536-well plates Wavelength range: Visible to 650 nm | 96-well (200 μL) 3pM 384-well (50 μL) 7 pM 1536-wel (8 μL) 20 pM \*Specifications | 96-well (200 μL) 15 pM 384-well (50 μL) 30 pM 1536-well(8 μL) 60 pM | 1 | | | | 0200-7015 | 384-well plates Wavelength range: Visible to 650 nm | 96-well (200 μL) 2 pM 384-wel (50 μL) 3 pM | 96-well (200 L) 10 pM 384-well (50 μL) 15pM | 1 | | | | 0200-7014 | 96-well plates Wavelength range: Visible to 650 nm | 96-well(200 μL) 2 pM | 96-well (200 μL) 8 pM | 1 | | Dual Color Luminescence (LUM) (BRET2) Detection Cartridge | ·Dual Color Luminescence (LUM) (BRET2) ·Detection Cartridge that isolates each well to provide optimal performance for chemiluminescence applications | 0200-7016 | EM1: 410/35 nm EM2:515/35 nm | | | 1 | | Transmitted Light (TL) Detection Cartridge (for use with the SpectraMax MiniMax 300 Imaging Cytometer) | ·Transmitted Light (TL) Detection provides white LED illumination for transmitted-light (brightfield) imaging with the SpectraMax MiniMax 30o Imaging Cytometer | 5022671 | Proprietary solid- state illumination, white LED | | | 1 | | Custom Solutions cs | ·Custom cartridges are available and designed to meet your specific research needs | Ask your local sales representative for more information | \* Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## GxP Support Plans # SMPCare GxP Support Plans Getremote PhD level technical support from the experts that know our microplate readers and SoftMax@ Pro $\\mathsf { G x P }$ Software best. Only Molecular Devices SMPCare provides one-stop support from experts who can resolve most issues in a single call.Additionaly, we have consultative services to help with protocol and SOP development for your organization. # Keybenefits ·Specialized support specifically for compliant labs ·Access to a team of subject mater experts for help with advanced software acquisition and analysis settings ·Access to software update installers released during your active plan term SoftMax Pro 7.1.x GxP and higher versions | | | | | | --- | --- | --- | --- | | Coveragefor Softmax Pro GxP Software Scope | No Plan Plan | | Software activations | Additional SoftMax Pro GxP Software activations\* per user per year | 0 | 2 | | Plate reader connectivity | Assistance with plate reader connectivity troubleshooting | · | | | Software function | ·Assistance with software functionality troubleshooting (<5 minutes) ·Remote screen sharing | | | | Software application support | ·Assistance and guidance on feature implementation and use (5+ minutes) ·Direct access to subject matter expert | | | | ·Access to software & document updates via SpectraNet Access to update installers released during active plan term | | | | Continuity maintenance | Access to service packs released during active plan term | | | | GxP Admin 3.x/7.2 Portal support · User/role management | Support for: ·Password reset account recovery | | | Starting from SoftMaxPro 7.1.1 ${ \\mathsf { G } } { \\times } { \\mathsf { P } }$ Software \* Applies to Multi Computer set up only \*\* Software updates may address potential cybersecurity issues.SMPCARE $\\mathsf { G x P }$ support does not include Validation services. # Coverage forSoftMax SoftMax Pro 7.1.x GxP and higher versions | | | | | | --- | --- | --- | --- | | Pro GxP Software | Scope | No Plan | Plan | | Validation support | Support and guidance for: ·Software Validation Package | | | | · Deviation and risk management Post-deployment guidance on installation of SQL database and | | | | Installation support Advanced formula | server questions Software support for data analysis functionality in existing protocols | | | | assistance Perfective maintenance | and formulas Optimize software performance per recommended server | | | | Adaptive maintenance | computer specifications Guidance for adapting our GxP software to changing operating systems | | | | Priority SoftMax Pro | | | | | INSIDER² Software Maintenance Plan | Priority notification of new alpha and beta testing programs | | | | | | Annual maintenance and technical support plan for SoftMax Pro 7.1.x GxP and higher | | Software Validation Service On-site software validation for SoftMax Pro GxP 7.1.1 Software or higher | | | \*Austria,eirkd,d,d,lyg, Portugal, Spain,Sweden,Switzerlandand United Kingdom 2Requires enrollment in SoftMax Pro INSIDER program # Additional paid support and services Product training Protocol building and support Custom coding/scripts Systemarchitecture consultation or specification System installation/upgradeassistance Data migration assistance # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## Automation Solutions for Assays ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/04782213013abec0298c8b2a446f34c1bf5a2cbf75028f585a7949379010634f.jpg) # Automation solutions for high-throughput plate-based assays :xplore our fully-integrated,automated workflow solutions for celland biochemical assays As timelines compress and the amount of data required for IND submissions and publications increases, laboratories are pressed to do more with les.The introduction of automated work cels can help relieve this burden by allowing scientists to optimize their time in the lab and obtain data more efficiently. We can build flexible,scalable,future-proof workflows backed by decades of consultative expertise to help you design and implement work cells that incorporate industry-leading technology to achieve your goals. # ELISA Workcell Walk awaysolution for ELISA's, cell based workandmore # ELISAAdvancedWorkcell Compact and flexible solution for a wide range of HTS work ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/deb9917ee8c3f18633a461c8f8d22d01f4eb50031f6c5b373b833de9a445b78b.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/be778002237df3f49d803dd2ae79a1c5243457b4738406cbacb22eccfa3cccd6.jpg) | | | | --- | --- | | PlateReader | SpectraMax iD5 multi-mode system | | PlateWasher | AquaMax4OooMicroplate Washer | | Robot | Peak Robotics S-LAB Plate Handler | | Plate Storage | Plate Hotels- 40 microtiter plates | | Controlling Software | Peak Robotics S-LAB Software | # Learnmore Learnmore | | | | --- | --- | | PlateReader | SpectraMax iD5 multi-mode system | | Plate Washer | AquaMax4OooMicroplate Washer | | Robot | PreciseFlex 400 Sample Handler | | PlateStorage | Plate Hotels- 4O microtiter plates | | Controlling Software | Genera Control Software | # ELISA Advanced EC Workcell # ELISA HTS EC Workcell Incubatorincludedto expand yourcapability Great for HTS assay work covering sample prep to analysis ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/7ad504db36bec696fb34c81620b66347cdede1c7e067968bb73f823a6695b3a8.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/948ab5b34ab92820800006c58f0160eee789115130d6420fe1c747fa8dcca361.jpg) # Learnmore Learn more | | | | --- | --- | | PlateReader | SpectraMax iD5 multi-mode system | | Plate Washer | AquaMax40ooMicroplateWasher | | Robot | PreciseFlex40o Sample Handler | | Plate Storage | Plate Hotels- 40 microtiter plates | | Controlling Software | Genera Control Software | | Incubation | LiCONiC STX44 Automated CO Incubator | | | | | --- | --- | | Plate Reader | SpectraMax iD5 multi-mode system | | Plate Washer | AquaMax40oo Microplate Washer | | Incubation | LiCONiC STX44 Automated CO, Incubator | | Liquid Handler | Microlab STAR Automated Liquid Handling | # ELISA HTSx Workcell Flexible and fast.No limit to throughput or capability ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/9feb6a60f49d6cbc9083e5af52fe4a49df6fff8d8d812e59d07d93d02df6a937.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/61082e7a11d8297c57e4815c3c03c75f5efb5b081cc49e09ebf93af6f2c1cad5.jpg) Learnmore | | | | --- | --- | | PlateReader | SpectraMax iD5 multi-mode system | | Plate Washer | AquaMax 4Ooo Microplate Washer | | Robot | PreciseFlex 40o Sample Handler | | Plate Storage | Plate Hotels - 4O microtiter plates | | Controlling Software | Genera Control Software | | Incubation | LiCONiC STX44 Automated COz Incubator | | Liquid Handler | Microlab STAR Automated Liquid Handling | # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check ourwebsite fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 sr ## Next-Gen SpectraTest ABS2 # Upgrade to the next generationSpectraTestABS2 AbsorbanceValidationPlate Validating the optical performance of your plate reader has never been easier. The new SpectraTest@ ABS2 Absorbance Validation Plate simplifies the certificate entry process to reduce human error and expands the testing capabilities to include more wavelengths and an extended linear dynamic range.Enhanced test methods and vendor recommended acceptance criteria ensure your readeris thoroughly tested against specifications we consider critical to achieving quality results. # Feature ABS1ValidationPlate ABS2 Validation Plate | | | | | --- | --- | --- | | Linear dynamic range (photometric accuracy and precision tests) | Up to 1.7 OD: all supported readers | Up to 3.0 OD: SpectraMax? iD3, iD5,ABS, ABS Plus readers Up to 2.0 OD: SpectraMax i3/i3x, M Series, Plus 384,and FlexStation@ 3 readers | | Wavelengths tested for photometric accuracy and precision (NIST and NMI traceable) | 440,465,546,590,635 nm | 405, 440, 465, 490, 546, 590, 635,650,750 nm | | Wavelength accuracy test range | 330\*-680 nm | 330\*-775 nm | | Certificate of calibration | Paper-based | Digital and paper-based | | ISO 17025 accredited recertification service | · | · | | 8+ tests to validate optical performance | · | · | | 8 replicates of each filter type tested | · | · | | Simple to understand test calculations and results | | · | | Improved test methods and statistical significance of results | | · | | Overall acceptable/out of specification result Automated EZinCert certificate value entry | | · · | | tomatiicopltainsswith | | · | | Updated packaging for easier shipping and handling | | | | ABS2 validation plate | Part number | | ABS2 validation plate (requires SoftMax@ Pro Software version 6.5.1 or higher) | 0200-6191 | | ABS2 recertification service | S9200-0186 | | Gray replacement case | 5074718 | \*Validated wavelengths are dependent on the reader model. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,eli,k,dre,yd,thsindd ## Recertify Your Validation Plate ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/40f267dc816da68cf4652c50e05d35dfde1c5b0a88466168861d3fa59643cd2b.jpg) # Why recertify your validation plate? Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Only Molecular Devices has the necessary knowledge and equipment to recertify SpectraTest? Validation Plates. ·Molecular Devicesis ISO17025accredited+ byAmericanAssociationof LaboratoryAcreditation (A2LA),whichrequires rigorous outside auditing onaregularbasis so thatyoucan beassured ofthe accuracyofyourcertficate with respect to NIST and NMl traceable standards. ·Gold Standard and Reference Standard Molecular Devices microplate readersare used forrecertification so that your certificate values are traceable. ·If validationplaterepairisneeded,onlyfactory-approvedpartsthatmeetourinternalspecificationsfortheseplatesareusec · Rapid turnaround time of five business days or less.Expedited service is available as needed. +View our ISO 17025 certificate of accreditation. # Benefits · Ensure data accuracy of your reader ·Monitorreader performance ondemand toensure dataqualityis notcompromised between PM/OQ maintenance services · Minimize downtime by quickly being able to distinguish between reader and assay issues · Maintain conformance to your validation protocols Ve developed your validation plate一you can trust us to provide you with high quality service at the best value. # Email us to schedule your plate recertification. North America: [Customer.Relations@moldev.com](mailto:Customer.Relations@moldev.com) Europe: [Service.EU@moldev.com](mailto:Service.EU@moldev.com) China: [Support.China@moldev.com](mailto:Support.China@moldev.com) Rest of World: Please contact your local sales representative. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Blgim,rk,id,re,ermnyed,therla,pindedid ## COVID-19 Research Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/f2f6fd1f7300b0780d00ee39417a32bcf0fa7770bf0b94764770be0c87fa8777.jpg) A flexible and reliable high-performance microplate reader for your virus research. # Nucleic acid quantification # Assays to quantify viruses and study viral processes # Cell signaling assays Measure nucleic acid concentration directly using absorbance;or use fluorescence for greater sensitivity Assess cellular response toviral infection Measure viral replication,virus neutralization # Cell viability Assess cell viability by measuring ATP levels or cellular enzyme activity # Biomolecular binding Study viral assembly,other viral processes; detect viral components,viral inhibition # Detection and quantification of specific proteins Detect and measure viral antigens,or immunoglobulin produced in response to virus exposure # Total protein quantification (non-specific) Measure protein (e.g., viral protein) concentration directly using absorbance; or use a colorimetric method for greater sensitivity Click here to contact your local sales representative to find out more. Committed to supporting scientists in their research of COvID-19 cellular response and vaccine development. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,eliu,rk,dre,rmyd,thr,indd ## SpectraMax ABS Microplate Reader ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/da3c5f723fa612db3ec5c563b8c80b7c0ae4a974882c76c96b282805523bb3c0.jpg) # SpectraMaxABSand ABSPlus Microplate Readers Small but mighty absorbance plate reader ·Affordable monochromator-based plate readerwith linearity of up to 3 OD ·Works with 96- and 384-well plates as well as cuvettes (ABS Plus) · Option to run samples as low as $2 \\mu \ L$ with the SpectraDrop" Micro-Volume Microplate ·Runs various assays within the Vis/UV-Vis range such as ELISA,Bradford,BCA,microbial growth,protein quantitation and more 19,400+ Citations on Google Scholar for Molecular DevicesAbsorbance Microplate Readers 10,700 Citations on Google Scholar for SoftMax Pro Software # Key features · Convenience: Flexibility to choose your wavelength Elimination of wel-to-well pipetting differences with Patented PathCheck@ Sensor · Ease-of-use: Preconfigured protocols such as ELISA and Bradford in SoftMax@ Pro Software Easy and fast assay setup and data analysis · All-around support Benefit from nearly 20,000 citations\* Get help with your work from our team of highly rated Ph.D. level scientists # SpectraMax ABS and ABS Plus Application Capabilities # Data analysis made easy ·Quickly export to Microsoft Excel or utilize SoftMax $\\mathsf { P r o } ^ { \\otimes }$ Software (4 licenses included) · $1 6 0 +$ preconfigured protocols (including those listed in the above table) ·Optimized analysis algorithms · $^ { 2 1 + }$ different curve fit options ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/5c40f53d70ccc92b2b556c035bce9ac3988d890adc8fba92e1b5493a0afbce57.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/c74e28e98ee0b07b19c3188bb39803d528283a7632ec0be51534016c8896cded.jpg) | | | | | | | --- | --- | --- | --- | --- | | Capabilities | SpectraMax ABS | SpectraMax ABS Plus | Wavelength | Pre-configured Protocols | | ELISA | · | · | Variable | · | | Bradford, BCA Protein Assay | · | · | 540, 590, 595 | · | | NAD/NADP Conversion | | · | 340 | · | | Alkaline Phosphatase (AP) ELISA | · | · | 405 | · | | β-galactosidase (Beta GAL) | · | · | 405 | · | | DNA/RNA Quantification | | · | 260,280 | · | | HrP)ELis Peroxidase | | | 492 | | | MTT & XTT Cell Viability Assay | · | · | 570 | · | | Lowry | · | · | 650 | · | | Diphenylamine DNA (DISHE) | · | · | 595 | · | ri # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check ourwebsite fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## COVID-19 Research Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/d56ccda1ad14ee32a2c0570961292b17d16f9fd76cc5e45523bb50d088855c00.jpg) A flexible and reliable high-performance microplate reader for your virus research. # Nucleic acid quantification # Assays to quantify viruses and study viral processes # Cell signaling assays Measure nucleic acid concentration directly using absorbance;or use fluorescence for greater sensitivity Assess cellular response toviral infection Measure viral replication,virus neutralization # Cell viability Assess cell viability by measuring ATP levels or cellular enzyme activity # Biomolecular binding Study viral assembly,other viral processes; detect viral components,viral inhibition # Detection and quantification of specific proteins Detect and measure viral antigens,or immunoglobulin produced in response to virus exposure # Total protein quantification (non-specific) Measure protein (e.g., viral protein) concentration directly using absorbance; or use a colorimetric method for greater sensitivity Click here to contact your local sales representative to find out more. Committed to supporting scientists in their research of COvID-19 cellular response and vaccine development. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,eliu,rk,dre,rmyd,thr,indd ## SpectraMax Mini Reader ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/34f658deb9cdb6f79f68f703af2cb6460f9a033a0245b333a58c6a740b6b8acc.jpg) # SpectraMax Mini Multi-Mode Microplate Reader Affordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expressionwithauser-friendly reader Withthree modes of detection forfullspectrumabsorbance,fluorescenceand luminescence,the SpectraMax@ Mini reader is userupgradeable—from 2to 3modes,providing researchers withaversatile instrumentthatdoes not compromise on sensitivityorperformance.Included SoftMax@ Pro Software enables rapiddata generationandanalysis helping you achieve more in less time. # Fluorescent DNA quantitation Determine DNA concentrations preciselyand accurately overa wide dynamic range # Protein quantitation Determine protein concentrations in cell lysatesand more ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/36a80aa9e8b486d3cc277186f41775712436685259393af200d1941e5f3ec22d.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/76ad322742c65ca2ab7469a815c75f6572e4bc759649afb3f8aead995e09148f.jpg) # Reporter gene assays Assess fireflyand Renilla luciferase activity overa broad range of cell numbersand expression levels ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/ffdc4f638d66008bd5ef520a8f79f0cc8514dae5071d3c37de0a966bc38e1082.jpg) # Cell viability Easily measure cell viability witha luminescencereadout ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/f2ce2ff21deb20617fcce2acbb06fb420f1aa82e83008579df023f8f047e4f15.jpg) # General specifications # Absorbancephotometricperformance # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. | | | | | | --- | --- | --- | --- | | Dimensions (in.) | 15.75 (L) x 12.60 (W) x 13.78 (H) | Wavelength range | 200-1000 nm | | Dimensions (cm) | 40 (L) x 32 (W) × 35 (H) | Wavelength selection | 1.0 nm increments | | Weight | ≤ 40 Ibs (18 kg) External 24V DC power supply compatible | Wavelength bandwidth | ≤5 nm full width half maximum | | Power requirement | with 100-240V AC @50-60 Hz 160W maximum consumption | Wavelength accuracy Wavelength precision | ± 2 nm across wavelength range ± 0.2 nm | | Robotic compatible | Yes | (repeatability) Photometric range | 0-4.0 OD | | General performance | | Photometric resolution | 0.001OD | | Plate formats | 6,12,24,4896,8a | Photomericacuracy. | < ±0.006 0D ±1.0%, 0-3 OD | | Light source | Two high-energy xenon flash lamps | Photometric precision | < ±0.003 0D ±1.0%, 0-3 OD | | Reading capabilities | Microplates,cuvettes (via adapter) | (repeatability) Stray light | ≤ 0.05% at 230 nm | | Detectors | Ultra-cooled Photomultiplier Tube and Silicon photodiode | Fluorescence intensity performance | | | Shaking | Linear, orbital, and double orbital | Wavelength range | 250-850 nm | | Temperature control | 4C above ambient to 45C | Dynamic range | \> 6 decades | | Temperature uniformity | ±1C at 37C | Sensitivity (fluorescein) | 1pM | | Temperature accuracy | ±2°C at 37C | Luminescence performance | | Spectral scanning | Abs | Wavelength range | 300-650 nm | | Endpoint reading | Abs, FI, Lum | Dynamic range | >7 decades | | Kinetic reading | Abs, FI, Lum | Sensitivity (ATP) | 2 pM | | Well scanning | Over 20 by 20 | Cross-talk | <0.1% (white 96-well half-area microplate) | ri USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## Legacy GxP File Import ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/32597ccf598eec25dbb2ae2af022bfa0f61bd30c48bb4d8bfa8cd12c18dbb17b.jpg) # File conversion and import function of legacy GxP software files To comply with regulated data retention requirements, management of all SoftMax® Pro Software (SMP) files is easier with SoftMax Pro $7 . 1 . 2 \\mathsf { G x P }$ Software edition which allows for importing legacy files into access-controlled folders into a secure SQL database (introduced as a data integrity enhancement in the 7.1.1 version). Legacy File Import allows single or multiple files (in folder) into database folders you can design for Data Archival. • SMP 4.0 – 5.4 Protocol files: (\*.ppr) • SMP 4.0 – 5.4 Data files: (\*.pda) • SMP 6 & 7 Protocol files: (\*.spr) • SMP 6 & 7 Data files: (\*.sda) • SMP GXP 4.3 – 5.4 GxP Protocol files: (\*.epr) • SMP GXP 4.3 – 5.4 GxP Data: (\*.eda) • SMP GXP 6.0 – 7.0.3 GxP Protocol files: (\*.sprx) Files not yet imported into the database • SMP GXP 6.0 – 7.0.3 GxP Data files: (\*.sdax) Files not yet imported into the database File Conversion into the latest software version file format occur during the import function: • SMP GXP 6 & 7 ${ \\mathsf { G } } { \\times } { \\mathsf { P } }$ Protocol files: (\*.sprx) • SMP GXP 6 & 7 $\\mathsf { G x P }$ Data files: (\*.sdax) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/e15c7cb9ddace1fd5b9c96701e21aef8ab8bee2109980490223a75bb378497a5.jpg) # Key features • Ongoing data retention requirement is maintained • Legacy file formats are converted into the most current format • Regulated data are imported into a secure database • Document audit trails are imported ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/46e5d4bed3d046a2b5b755dfc7223ac417ef24b91d4078057bb25b3c625c66a1.jpg) Original PDA file when opened in SoftMax Pro 5.4 GxP Software # Converted into SDAX file when opened in SoftMax Pro 7.1.2 GxP Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/5210cd57d6915d4e7f49f2339df686f9f1db55675a4f7455c3380b880f4a3ae9.jpg) Curve Fit Results Curve Fit:4-Parameter Logistic y=D+ $y = \\textit { \\textbf { D } } + \\frac { \\textit { \\textbf { A } } - \\textit { \\textbf { D } } } { 1 + \\left( \\frac { x } { C } \\right) ^ { \\frac { \\beta } { B } } }$ | | | | | | | --- | --- | --- | --- | --- | | | Parameter | Estimated Value | Std. Error | Confidence Interval | | Std R² = 0.999 | A | 9202 | 69.49 | \[9055,9350\] | | EC50 = 0.594 | b | 2.222 | 0.077 | \[2.058, 2.387\] | | | c | 0.594 | 0.010 | \[0.574, 0.615\] | | | D | 469.1 | 65.27 | \[330.8, 607.5\] | Starting with SoftMax Pro $\\mathsf { G x P }$ Software version 6.0, the calculations done by the software on real numbers use 64-bit, double-precision $1 5 +$ digits of precision) as defined by the Institute of Electrical and Electronics Engineers standard for floating point arithmetic (IEEE 754). Computations always use the maximum available precision. Rounding occurs only as required for display formatting purposes, or in the evaluation of a formula in which the round operator is used. Note: In SoftMax Pro Software versions prior to version 6.0, the software used a text-conversion algorithm with a slight upward bias. This can result in a mismatch between the rounded values in the newer version and the older version. Example: When rounding the raw value 14.849999… to one decimal place, versions 6.0 and newer display 14.8, while older versions display 14.9. This does not affect the accuracy of the calculations, since rounding occurs only as required for display formatting purposes, or in the evaluation of a formula in which you use the round operator. Computations always use the maximum available precision. Starting with SoftMax Pro Software version 7.1, the Legacy Rounding option improves consistency with the SoftMax Pro Software version 5 which introduces a small factor to force rounding upward. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/153398ca06dca1709b0346ef842f8cfd00693dfd0e60f8241dab5900540785e6.jpg) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ## Secure Data Sharing ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/18f2a53c4d705fc97cec9e1b7c5bcc3427a0ad861c77bb1148ad1889560abe90.jpg) # Sharing data between users has never been more secure ·Are bottlenecks an issue when multiple users in your lab try to access secure data from a singlecomputer? ·Is data integrity a concern but you have limited knowledge or resources to achieve FDA 21 CFR Part 11 or EU Annex 11 compliance? ·Are you spending valuable time trying to set up a secure environment instead of doing science? # If you answered yes, you are not alone. Many labs grapple with these questions daily. In a quest to become compliant, labs depend on their IT team for setting upa secure networked environment. Our SoftMax? Pro ${ \\mathsf { G x P } }$ Software Suite can scale up from a single computer to a global networked environment.Our Professional Services team will partner with your IT team to planand implementa secure networked SoftMax Pro ${ \\mathsf { G } } { \\times } { \\mathsf { P } }$ Software application suite according to your requirements. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/695d0f50f2303e30c6d01916467f9786c2d57c5f0d1f7a0caa03dbddf6f4529b.jpg) # Benefits of a secure networked environment · Secure,reliable,centralized data repository for easy data sharing一protectsagainst data lossand minimizes failure compared to desktop hardware · Reduced user management tasks一can control projects, user permissions,and accounts from a single location · Consolidated, comprehensive system audit trails help prevent audit complications · Valuable time freed up to focus on research instead of server installation ormaintenance · IT department not required to be a part of the data access processafter installation | | | | --- | --- | | Installation type | Multi computer | | Number of networked computers | Multiple | | Number ofmicroplate readers | Multiple | | Configuration | SoftMax Pro GxP Admin and database can be installed on different computers | | Database | Microsoft SQL Express (SQL Server Standard or Enterprise edition not included) | | File sharing | Yes.Files can be shared with any computer connected to the database.The system audit trail remains intact. | | User ID and password control | Active Directory Windows Credentials or GxP Admin software | | Installer | Script based installation by our Professional Services team Client computer · Windows 10 Pro/Enterprise 64-bit | | Computer requirements | ·Windows PowerShell 5.1 Server computer ·Windows Server 2016 or 2019 · Intel Core i5 or higher with 8 cores · 16 GB RAM · 50 GB of hard drive space ·Microsoft SQL Express or SQL Server 2016 or 2017 ·Windows PowerShell 5.1 Configured DNS active for all connected computers · Firewals and routers configured to allow data transfer using ports 8210,821,1433,and 1434 | | Software part number Software remote installation service part number | SMP712GXP-SVR-LIC | | Customized installation service | SMPGXP-INSTALLSVR-OS | | | SMPGXP-INSTALLADVSVR-OS | | Additional client computer installation service | SMPGXP-INSTALLCLT-OS | | Additional user license part number | SMP GXP SVR ADD | | Software maintenance | ·SMPCARE-GXP-ESSENTIAL SMPCARE-GXP-PREFERRED | The MultiComputer InstalltionServiceincludesaconsultationcallwithour ProfessionalServicesteam toensurethe softwareand installtion meet yourIT infrastructure needs. Please begin to discuss the following topics with your IT support team. · Contact information for IT group if they will be involved with the installation ·Server type:bare metal or virtualized (Hyper-Vor VMWare) ·Internet access for remote installation ·Server information ·SQL database may be colocalized on the same server as the ${ \\mathsf { G x P } }$ Admin software or on separate servers ·SQL database information ·Single site,multisite,or global database access ·Active directory integration · Installation service package type ·Standard oradvanced # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 Austriaedd ## Maximize Your Productivity SERVICEPLANS # Maximize your productivity # with our expert engineers and flexible service plans Each instrument's functional level will evolve over its operational life cycle一changing with the laboratory environment,assay conditions,usage,role in experimental workflow, compliance requirements,repair,and maintenance needs. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/00771ed9a4b3d0018409e7336d46473131584b9863cee086e77b2f5ca42c1ef5.jpg) \[oaddress these evolving functionallevels,weoferseveralflexible service plans that provide comprehensive support or your microplate readers and washers backed bya global network of our certified expert engineers.\ \ | | | | | |\ | --- | --- | --- | --- | --- |\ | Service Plans\* | Compliance Assurance Plans (incl. PM/OQ)\*\* | Performance Assurance Plans (incl. PM) | Plans (No PM) | Essential Protection |\ | On-site orMaintenance& Repair Center (MRC) Service Depot | On-site | On-site | MRC | On-site MRC |\ | Unlimited service calls\*\*\* | Yes | Yes | Yes Yes | Yes |\ | Factory-approved parts included | Yes | Yes | Yes Yes | Yes |\ | Service Engineer labor hours | Yes | Yes | Yes Yes | Yes |\ | Preventive Maintenance (PM) | Yes | Yes | Yes 二 | 二 |\ | Operational Qualification (OQ) | Yes | 二 | 二 二 | 二 |\ | OQ following corrective maintenance | Yes | 1 | 1 1 | 一 |\ | FieldService Engineer travel | Yes | Yes | N/A Yes | N/A |\ | Shipping costs to Maintenance & Repair Center (MRC) Service Depot | N/A | N/A | Yes N/A | Yes |\ \ \*Custom service plansare also available. \*\*Recommended for GxP labs. \*\*Teservicepansresbjecttoteolelarevicessevetsalablere:htps://wmoleclardevcesom/termsanddiio\ \ Priorityrespoeariesbyegiandspeifieiceagreemntaseosulturegioseiceandsupportentefodtioalfoatio 2 For selected instruments only and subject to availabity.\ \ # Choose the right service plan for your specific laboratory needs.\ \ # Compliance Assurance Plans (PM/OQ)\ \ Unlimited service by our trained and qualified field service engineers\ \ ·Molecular Devices factory-approved parts\ \ ·Parts,labor and travel for on-site service covered at $100 %$\ \ ·Available for on-site coverage only\ \ ·Priority response1\ \ ·Annual Operational Qualificationwith Preventive Maintenance service per readerorwasher\ \ # Performance Assurance Plans (PM)\ \ Unlimited service by our trained and qualified field service engineers\ \ ·Molecular Devices factory-approved parts\ \ Parts,labor,and travel covered at $100 %$ for on-site coverage\ \ Parts,labor,and shipping covered at $100 %$ for MRC coverage\ \ ·Priorityresponse1\ \ Annual preventive maintenance Loaner unit²\ \ # Essential Protection Plans\ \ ：Unlimited service by our trained and qualified field service engineers\ \ ·Molecular Devices factory-approved parts\ \ ·Parts, labor,and travel covered at $100 %$ for on-site coverage\ \ ，Parts,labor,and shipping covered at $100 %$ for MRC coverage\ \ Priority response1\ \ ·Loaner unit²\ \ # Contact [serviceplans@moldev.com](mailto:serviceplans@moldev.com) for more information.\ \ # Contact Us\ \ # Regional Offices\ \ Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors.\ \ USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,Belgium,ark,d,rce,ermnyed,etherlad,pinwededid ## Luminescence Sensitivity Comparison # SpectraMax Microplate Readers Comparing luminescence sensitivity of SpectraMax microplate readers using a homogeneous ATP assay # Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. \#6016736) ATP standards ranging from $1 \\mu \\mathrm { M }$ to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). 50 µL of standard was pipetted into the wells of a white 96-well half-area microplate (Corning cat. $# 3 6 9 3$ ; ${ \\mathsf n } = 4$ for each standard). Blank wells containing 50 µL culture medium only $( \\mathsf { n } = 8 )$ ) were also prepared. $5 0 ~ \\mu \ L$ of ATPlite 1step reagent was added to each standard and blank well. Contents of wells were mixed for 2 minutes using a plate shaker. Luminescence was then measured using the microplate readers listed. An integration time of 1 second was used for each reader. Standard curves were plotted using SoftMax® Pro Software (Figure 1). To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing $5 0 ~ \\mathsf { n M }$ ATP (sample). The lower limit of detection (LLD) for ATP was estimated for each microplate reader using the following formula: LLD $= 3 ^ { \\ast } \\mathsf { S D } \_ { \\mathsf { b l a n k } } ^ { \\ast }$ Concentration/(RLUsample-RLUblank). Results are shown in Table 1. # Instruments compared • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/9004d6b70688d301fd750110c8ccaab67bf0b989fffff66c5400c64ead2dcf7a.jpg) Figure 1. ATP standard curves read on SpectraMax microplate readers. Table 1. Lower limits of detection for ATP determined using the ATPlite 1step assay with SpectraMax microplate readers. | | | | | --- | --- | --- | | Instrument | ATP LLD (pM) | ATP LLD (moles) | | SpectraMax i3x,luminescence detection cartridge LUM96 | 59.2 | 5.9 × 10-15 | | SpectraMaxi3x, onboard luminescence | 121.3 | 1.2 × 10-14 | | SpectraMax iD5 | 81.3 | 8.1 × 10-15 | | SpectraMax L | 36.5 | 3.6 × 10-15 | | SpectraMaxM5e | 118.1 | 1.2 × 10-14 | | SpectraMax Mini | 17.8 | 1.8 × 10-15 | Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current list­ ing of worldwide distributors. USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China +86.4008203586 Taiwan/Hong Kong +886.2.2656.7585 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 India +91.73.8661.1198 ## Software Validation Services Our SoftMax Pro GxP Software Validation Test Suite provides the most comprehensive documentation available to validate GxP functionality that ensures data integrity and compliance to FDA 21 CFR Part 11 and EudraLex Annex 11 and Annex 15. Each step in the process will be carefully planned and executed. Software Validation Test Suite: SoftMax® Pro Data Acquisition & Analysis Software – GxP Edition Key benefits • Minimize downtime - leave the validation to us and focus on your research • Gain access to a pre-service consultation led by our Professional Services team • Get comprehensive software validation testing by our certified Field Service Engineer (FSE) • Streamline the IQ and OQ validation activities to one day with our automated service tools • Receive a complete data package of audit-ready documents after completion of all tests: • Electronically signed, digitized copy of SoftMax Pro GxP validation document \[IQ, PQ\] • Automated OQ: Results template with select screenshots at each test case • Copy of the FSE Training Certificate Validation Test Suite Whether you decide to validate for yourself or purchase our software validation services, our Validation Test Suite provides step-by-step instructions with complete and accurate “soft copies” of required IQ/OQ/PQ records can be printed to document activities for your regulated environment. Visit Spectranet, our Customer Portal for the current version of the SoftMax Pro Software Validation Test Suite. • Installation Qualification \[IQ\] worksheets contain instructions for installing the SoftMax Pro GxP Software Suite in stand-alone (single PC) or single server (multi PC) environments • Operational Qualification \[OQ\] worksheets contain a comprehensive suite of functional testing of all configurable permissions in the GxP Admin Portal and their behavior in SoftMax Pro GxP Software • Performance Qualification \[PQ\] worksheets contain a suite of performance tests for connectivity, curve fitting, and graphing interpolation using sample data sets in SoftMax Pro GxP Software The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ©2024 Molecular Devices, LLC 3/24 2316B Printed in USA Phone: +1.800.635.5577 Web: www.moleculardevices.com Email: info@moldev.com Check our website for a current listing of worldwide distributors. \\* Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom Regional OfficesContact Us USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China +86.4008203586 Taiwan/Hong Kong +886.2.2656.7585 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 India +91.73.8661.1198 ## SoftMax Pro Tools ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/c79f53857f8a258c468d4e5d5ab7fb941e542b3609aa8ecd8d7f00484b874a8d.jpg) # SoftMax Pro Software Import and Export Tools The import and export functionality of SoftMax® Pro Software allows for importing plate templates as xls files which are saved in a .txt format. Plate templates can be exported in a text or directly in a xls format. Plate data can be imported in two different ways: first by manually pasting the data into a Excel template file (or using a macro), second by saving it as .txt file and importing it into the software. The template file can be found in the software directory. Alternatively a programmer can write a script that formats the data according to the .xsd import scheme and generates an xml file. This file can then be imported into SoftMax Pro Software. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/8a36dbc418a7f1dbe21f5e4635e61fdbeba8f79f7c5bffa4ab08497ed471b7db.jpg) 1. ``` Can be generated by exporting a plate template from SoftMax Pro ``` 2. ``` C:\Program Files (x86)\Molecular Devices\SoftMax Pro X.X.X. $_ { \mathsf { G x } }$ P\Plugins\Import-PlateFormat\Templates ``` 3. ``` C:\Program Files (x86)\Molecular Devices\SoftMax Pro X.X.X. $\mathsf { G x }$ P\Plugins\Import-XML\Schema ``` # Import your plate templates as well as data from third party devices. # Plate template import and export For plate templates, SoftMax Pro Software provides an inbuild template importer and exporter. The table below shows the general format of a plate template table. Plate template import and export format. | | | | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | | Well location | Group name | Group type | Sample name | Descriptor name (1) | Descriptor value (1) | Descriptor units (1) | Descriptor name (2) | Descriptor value (2) | Descriptor units (2) | | A01 | PBlank | | | | | | | | | | A02 | Standards | Standards | 1 | Concentration | 1 | mg/mL | | | | | A03 | Standards | Standards | 1 | Concentration | 1 | mg/mL | | | | | A04 | Unknown | Unknown | 1 | Dilution Factor | 1 | | | | | Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland # Data import and export Plate data import plugins for Excel or XML based data are separately available modules for SoftMax Pro Software. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/52a3a823776bdbee3bf0fb0e369278bd3f399ad1a8bb791974ea366c7cce6c43.jpg) # Excel-Based Import XML-Based Import Simply cut and paste your data into the provided Excel template and click to import. Automate the process using Excel import macros and scripting. Icons for plate template import in SoftMax Pro Software. | | | | | | --- | --- | --- | --- | | Product | Description | Perpetual (non expiring) | Subscription (annual) | | SoftMax Pro Importer XLS | Import of data into SoftMax Pro from an Excel-based template. | | SMP.IMPORT.XLS (Can be installed on 1 computer) | | SoftMax Pro Importer XLS and XML | Import of data into SoftMax Pro from an Excel-based template or XML file. | SMP.IMPORT.XLS.XML.NONEXP (Can be installed on 1 computer) | SMP.IMPORT.XLS.AND.XML (Can be installed on 1 computer) | Have your computer programmer write an application to convert your data into the provided $\\mathsf { \\times M L }$ schema for automated import. Custom Import Module Our experienced software developers will create a custom import module to meet your data handling needs. Fully automate your data import and analysis process with custom protocols. # Plate data import can be found in the plate menu. # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. Regional Offices USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.3971.3530 Japan (Osaka) +81.6.7174.8331 Japan (Tokyo) +81.3.6362.5260 South Korea +82.2.3471.9531 $\\circledcirc$ 2018 Molecular Devices, LLC 5/18 2188A Printed in USA ## Instrument Validation Services # Instrument validation services ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/f8c56d58db66b3193b9e8514fa8ee332ae1210ca91265e59ff64722996899595.jpg) # IQ/OQ and PM/OQ services Installation, operational qualification, preventive maintenance Assure ongoing compliance of your Molecular Devices microplate readers and washers and be audit ready with comprehensive validation and maintenance documentation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/bddaf306fc1db30e470418cc2c9a988d6a0d9eeef8eb4e417bc027b41d9d1d6b.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/2826888e85a523dc5fa05c3df84af2060aaa4c2773787de1ee3183111080f179.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/875081245b219a806f625fc6c4b6cd2eda702c14bf857a892414c9fff1984259.jpg) # Installation Qualification (IQ) Preventive Maintenance (PM) Verifies and documents that all necessary components required for operation are received and properly installed in accordance with Molecular Devices installation protocols. # Operational Qualification (OQ) Tests every subsystem of the reader orwasher including mechanical, electrical,and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Eachplate reader orwasher is calibrated,inspected,and lubricatedany potential issues are proactively addressed.Our comprehensive multipoint inspection and testing ensures each microplate readerorwashermeets our product operational specifications. # Operational Qualification (OQ) Tests every subsystem of the reader orwasher including mechanical, electrical,and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. # UV/OQ Service Ask about our enhanced qualification capabilities that now cover UV wavelengths from $2 2 0 ~ \\mathsf { n m }$ to $3 5 0 ~ \\mathsf { n m }$ The service validates and confirms: ·Photometric accuracy, precision and linearity ·Wavelength accuracy · Stray light # Key features · Automated validated calculations for accurate and faster results · Electronic signature with date/time stamp for compliance assurance · Secure archiving of IQ/OQ and PM/OQ report in digital and compliant format for on-demand access # Comprehensive digital data package Upon service completion,a comprehensive digital report is generated and provided.The report is also securely stored in a central location at Molecular Devices. Each report contains: ·Fully electronic document with eSignature,date,and time stamp that meets SAFE-BioPharma standards ·Operational Qualification results ·SoftMax Pro instrument data files · Instrument service report · Calibration certificates for validation plate and certified tools · Training certificate for your specific Service Engineer # Why we are your preferred service provider ·Fully trained and certified Field Service Engineers: ·Use proprietary software, tools,and OEM parts ·Provide comprehensive multipoint inspection and testing ·Maintain active certifications through continuous training · Access to expert technical support and a comprehensive knowledgebase ·Single provider for your service,applications,and sales needs # Ordering information Microplate reader services Part number | | | | --- | --- | | IQ/OQ service | Ending in “-IQOQSVC-OS" | | IQ/OQ service + UV Wavelength Operational Qualification | Ending in “/UV-IQOQSVC-OS" | | PM/OQ service + UV Wavelength Operational Qualification | Ending in“/UV-PMOQSVC-OS" | | Upgrade to perform IQ/OQ or PM/OQ on the customer computer instead of the Field Service Engineer computer | Ending in “-CUSTCPUOQSVC-OS" | | As-found service | Ending in “-ASFOUND-OS" | | PM/OQ service | Ending in “-PMOQSVC-OS" | | Additional preventive maintenance services | Ending in “-PMSVC-OS" | # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing ofworldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria,eli,k,dre,yd,thsindd ## SoftMax Pro GxP Guide ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/e54ed3161338f7f61a81a63d2f65cf021c28a6598795a93dc0c6a6ce20d437d2.jpg) PURCHASE GUIDE # SoftMaxPro GxP Software Suite The SoftMax@ Pro GxP Software Suite can scale from a single computer to a networked environment of multiple computers. Consult with your Molecular Devices representative and your network administrators to plan and scale your implementation according to your requirements. # Single Computer Setup ·Best for small labs with all users sharing one microplate reader ·All software components are installed on a single computer,which is used by all users for data acquisition, analysis,and user administration ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/727dff8324cd7f7e3ec57e28691302692427265619907fc0f0116f13fd3e054e.jpg) # Multi Computer Setup ·Best for labs with all users sharing multiple microplate readers through a server ona corporate network ·Data acquisition,analysis,and user administration may occur on multiple computers,which all connect to a database hosted ona central server ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/7d0673221c941a599ac484ddf0025a0ebd5d96fc99a97df1255db97e90634029.jpg) # SoftMax Pro GxP Software Suite components · $\\mathsf { G x P }$ Admin Software—Database and securitybackend application that youinstallon a dedicated,centrallylocated server with a static IP address. This server must be accessible to allcomputers that run the $\\mathsf { G x P }$ Admin Portal Software and the SoftMax Pro ${ \\mathsf { G } } { \\times } { \\mathsf { P } }$ Software. · $\\mathsf { G x P }$ Admin Portal Software-The user interface that you use to interact with the ${ \\mathsf { G } } { \\times } { \\mathsf { P } }$ Admin Software. Install the $\\mathsf { G x P }$ Admin Portal Software on a computer or computers that have access to the dedicated server on which you installthe $\\mathsf { G x P }$ Admin Software. · SoftMax Pro $\\mathsf { G x P }$ Software-Controls the microplate reader for data acquisition and statistical data analysis.These computers must have access to the dedicated server on which you install the ${ \\mathsf { G x P } }$ Admin Software. | | | | | --- | --- | --- | | Installationtype Single computer | Multi computer | | Concurrent users | One | Multiple | | Number of readers | One | Multiple | | Configuration | SoftMax Pro GxP, Admin and database installed on the same computer | SoftMax Pro GxP, Admin and database installed on different computers | | Database | Microsoft SQL Express with 10 GB limit | Microsoft SQL (not included) | | File sharing | No | Yes. Files can be shared with any computer connected to the database. The system audit trail remains intact. | | Database backup | Tool supplied | Not included | | User ID and password control | GxP Admin software | Active Directory Windows Credentials or GxP Admin software | | Installer Computer requirements | Simple wizard ·Windows 10/11 Enterprise or Windows 10/11 Pro (all 64-bit) · Intel Core i5 or higher with 8 cores (minimum 4 cores | Script based installation Client computer ·Windows 10/11 Enterprise or Windows 10/11/Professional Windows Server 2016 or 2019 (all 64-bit) | | GxP Software | Latest version of SoftMax Pro 7 GxP Software Suite includes: 1software activation for each userlicense,GxP Admin Software,self-guideddocumentationforsoftware lQ/OQvalidationpackage,userlicensecertificate,compliancecertiate, | Server computer ·Windows Server 2019 or 2022 (all 64-bit) · Intel Core i5 or higher with 8 cores (minimum 4 cores to install) · 16 GB RAM · 50 GB of hard drive space ·Microsoft SQL Express or SQL Server 2019 or 2022 ·Windows PowerShell 5.1 must be 64-bit. Must allow script execution with the execution policy set to at least Remote Signed Configured DNS must be active for all connected computers | | Software installation | Windows 11 compatible Standard server installation (Applies to initial purchase only) | | service Customized installation | Custom server installation for multi computer setup only | | service Additional user license | Additional user license and software activation purchases | | Software maintenance | Annual maintenanceand technical support plan for SoftMax Pro 7.1GxP Software or higher (Required forfirst year of new license purchase) | | Software validation service | Onsite software validation for SoftMax Pro GxP 7.1.1 or higher | "eMltiComallliofsutwial T infrastructure needs. Please begin to discuss the following topics with your IT support team. ·Contact information for IT group if they will be involved with the installation ·Server type: bare metal or virtualized ·Internet access required for remote installation by Teams,Webex, or Zoom ·Server information SQL database may be colocated on the sameserveras the $\\mathsf { G x P }$ Adminsoftware oronseparateservers SQLdatabase information Single site,multisite,or global database access ·Active directory integration · Installation service package type ·Standard or advanced # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check ourwebsite fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria,Bgium,mrk,d,rce,Grmyd,d,alyemourg,t Portugal,Spain,Sweden,Switzerland and UnitedKingdom ## SoftMax Pro GxP Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/1919a490d674a73eaaeff8a1d134cf387e2ec8a437f7a8bb93851f559b6935be.jpg) # Ensure data integrity,compliance,and audit readiness 一 in line with the latest FDA Guidance ;oftMax@ Pro ${ \\mathsf { G x P } }$ Software is the latest, most secure software to help you achieve fullFDA 21 CFR Part 11compliance with itreamlined workflows to ensure data integrity. Every step is optimized to simplify analysis and reporting. Our expert team will partner with you to support your GMP/GLP regulated work. # Benefits across your organization ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/1f0aecc50f026c0b37a7368115f8e1b89901f6037eee36f8e02e097919125b9c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/9e6b42d1d3ab9a9a72684d4e33edc4588cd364f757e2435f51b8681bbc838ddf.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/65ae6c82075f0c99371f56f7966e1287acdaa680624ca746a49d62507c511952.jpg) # IT ·Compatible with Windows Active Directory (server) · Single portal for user admin,including: ·Creating project folders with custom permissions ·Enterprise-level document sharing ·Data generated ina software validated for Windows 10/11 # Quality assurance and compliance ·System audit trail tracksand records all user actions in the database ·Documentaudit trail tracksand records all useractions in the SMP data document ·Enforces e-signature at each stage of the document workflow ·Featuresanewhistorical rawdata feature that tracks overwritten data ·Improved auto-save functionality ·Ensures that the most up-to-date protocols are always in use # Scientist ·Easy to use, protocol-based software for reproducible results ·Powerful data analysis options to create or import validated protocols from collaborators ·Each user only sees the functions they are permitted Reassurance you are using the most published microplate readercontrolanddataanalysis software in a regulated,secure environment ·Technical Support # ailure to meet the 2016 update to FDA Guidance may impact your ability to pass External or Customer audits. re you or your QA Manager looking forimprovements in your curent microplate validation or data management system? ontact us today to learn how we can help! # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 r ## MDCare Support Plans ![](https://www.moleculardevices.com/sites/default/files/en/assets/flyer/br/images/654fa89f894717b4ac5c10c1c40923e9a73a0b14a97ff3235b9406fb4d32daf4.jpg) # MDCare Support Plans # for microplate readers,Washers,and liquid handling systems MDCare"Support Plans provide you peace-of-mind priority accessto our qualified Technical Supportteam,help with using featuresofyour microplate reader,washer,liquid handling system,and more.Includedin the purchase priceofall microplatereadersisaone-yearlimited warrantyandourMDCare Ph.D.Plan.Aditionalplanoptionsare available shouldyou ned extended coverage and/orsupport after thefirst year.Each planoptionprovides assurance that we willbe available to help you whenit's most important.Simply reviewthe chart belowand pick the levelofsupport that suits your needs. # Years 2-3 Year 1 Instrument warrantyand MDCare Ph.D.Plan included inyear 1 Extended technical support can be purchased through any of our MDCare Plans | | | | | --- | --- | --- | | MDCare Ph.D. Plan | MDCare GxP Plan | MDCare One | | Includes all of the MDCare Support Plan features PLUS: ·Advanced analysis support (core features) · Up to 2 days of lab support (Molecular Devices site only) | Includes all of the MDCare Ph.D. Plan features PLUS: ·Advanced support specifically for compliant labs with validation questions on microplate readersand washers | Single incident coverage that includes: · Up to 90 minutes of Ph.D. qualified active support · Data and instrument setting reviews ·100% of value can be credited towards | # Contact Molecular Devices Technical Support at 1.800.635.5577 for more information and program details. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria,Belgium,enmark,Finand,France,Germany,Ieland,Netherlands,SinSwedennditzrd ## ClonePix 2 System ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/4ff43addc8bb26f54414d13f6669d8ababc8859eb1228ea9acd8c06ff152227a.jpg) # ClonePix 2 # Mammalian Colony Picker enhanced for monoclonality verification Tailored to your fit your workflow needs # Same ClonePix workflow, now enhanced with high resolution single cell imaging capability on day 0 The enhanced ClonePix 2 system can automatically screen and pick clones that are both high producing and monoclonal—all in one system\* Obtain a comprehensive view of isolated single cells both in XY and Z dimensions on day 0 with the rapid Z-stack acquisition feature, which takes ${ 2 , 4 0 0 + }$ high-resolution, Z-plane images per microwell and rapidly compiles them into an image stack. On the day of picking, colonies of interest are identified based on productivity data generated from the enhanced ClonePix™ system. These colonies’ origins can be traced back to single-cell images from day 0, allowing seamless assessment of a colony’s monoclonality and productivity prior to picking. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/85e9e8eea304801fc4039a4d89aa74eaebda471b081db4d9a0f1b29919706f14.jpg) Day 0 # Day of picking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/0303d3a24a8545630678915b000871c736705ad53adc451b4689de41f37e4829.jpg) The enhanced ClonePix system links high-value colony with its day 0 image for automated assessment of single-cell derived clones. # Key benefits • Reduce screening time from two rounds to one by providing image-based evidence of clonality • Rapid Z-stack acquisition feature allows detection of single cells throughout the medium volume, not just a single focal plane, on day 0 • Simplified workflow from single cell identification and productivity screening with the all-in-one system # Automated determination and picking of single-cell derived clones using the enhanced ClonePix 2 system # Day 0 imaging # Day 1–14 imaging # Day of picking Plate cells at low density into semi-solid medium Z-stack imaging on day 0 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7bce72aacdd7db8b737d0aa4a62755147d3ab5cb6021df693ac96ecce51d090f.jpg) Single cells are compartmentalized by semi-solid medium ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/f708892aeb901e4439853510f6939da4b67e63f5a3d789804843c3916e85d9ee.jpg) User can select specific locations or whole well to capture Z-stack ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9f3e6d750e11dc99283dcaa0b66616ef3e2396184e012f307fa1139d99554039.jpg) Z-stack acquisition at a single field-of-view Let colonies grow for a number of days; optional imaging on subsequent days during this period ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fadce95d1a5f971af2356af8d0784b71dc22f0a7cc18ff55196d9a0002bf710c.jpg) Clone productivity can be observed based on fluorescence intensity Screen and pick high-value clones based on fluorescence ranking and single-cell origin ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a8882c65796f147c1eb3c43b7bf5a2a135891874101008765ca4e6c6ca2fdb18.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ea39f3a705069f94dbdbac424e0edb5f4bf1310224715125277667d3c4548b7b.jpg) Determining single-cell derived colonies ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/1be9d51d4ea2467cd5e16baf45c359fcfde15f6d0f7f4b6934f26732c44899a1.jpg) Fusion software associates colony with day 0 images for automated assessment of singlecell derived clones. User selects the clones that will be picked. # Picking single-cell derived clones made easier Associating single-cell image data acquired on day 0 to the colony growth (yellow outline) allows evaluation of a clone’s origin prior to picking. An algorithm assigns a monoclonality confidence score to all high-value clones. Users can pick clones that are monoclonally derived with confidence. This enhanced ClonePix approach can reduce the timelines by half as the need for a second round of cloning to demonstrate clonality is no longer necessary. # Monoclonal Not monoclonal ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/daed2267863ea73871705b6624d87059ff344fa49916dd954c782c8d444e3a39.jpg) Certainty score $= 0 . 9 2$ ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fc2093eb8bccb6e1a8aa47dc83d379643cdc721d25554952546037dc077aa467.jpg) Certainty score $= 0 . 1 1$ Certainty score $= 0 . 0 6$ Fusion software assigns a certainty score to each colony based on picking day colony location and single cell Z-stack images at day 0. The threshold of determining monoclonality from the certainty score is variable based on user-defined inputs. # Accelerate cell line development timelines by integrating multiple steps into a single step | | | | | | | --- | --- | --- | --- | --- | | | | Stable transfection | Pool enrichment | Single-cell isolation | growth, productivity, screening | Titer and CQA characterization Scale up | | | | | | | | | | | | | # Explore modifications to fit your needs The Molecular Devices team has successfully tailored the ClonePix 2 systems for customers on request to include customized software and hardware\*. # Turnkey service Our team works with you to match our service offerings with your needs. Our solutions are tested and validated in-house before being installed in your lab. \* Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. Custom solutions are subject to Molecular Devices Custom Products Purchase Terms available at [www.moleculardevices.com/custom-products-purchase-terms](http://www.moleculardevices.com/custom-products-purchase-terms). ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9adc00f72212dff24b678a8d4e52ea85de62d294c7fec8dbe0af5b12de74339b.jpg) # Software customization We can match specific API needs, enable unique workflow capability, integrate into LIMS or data management systems, customize a UI, and/or control any custom designed hardware. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ef218e7dd1236163fc01aea2f2d86bd18f1e3e635cdb77eb8f0907ef9af5df48.jpg) # Hardware customization From custom labware to special handling requirements, we’ll work to find a solution and prove it through validation. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ## Accelerate Cell Line Development # Accelerate cell line development Get to your high value hits faster # Transform your workflows to elevate productivity # Fast screening and selection of secretory cell lines0wit 1h th2e Cl3onePix™ 2 Mammalian Colony Picker Screen 10X more clones in weeks, not months. Productivity comparison: ClonePix: 10,000 clones in three weeks, Limiting dilution: 1000 clones in two months. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/4094eea76363e2c57587609a0653268b6693cd07769418846b1c983016d5b6b6.jpg) # Select and pick with more accuracy and confidence Cells plated into semi-solid medium # User selects colonies based on system’s analysis and ranking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/18c95f7dfbf9ba055ca4e88590a9e9de109894d3e1c4d3c05b6bb58dcd44a442.jpg) # Advance to automated screening with intelligent imaging and quantitative analysis # Assessment of colony outgrowth with CloneSelect Imager™ replaces subjective, time-consuming manual screening Following screening and selection of clones, the ultra-fast CloneSelect Imager provides objective assessment of cell growth. Track and quantify cell growth over time automatically. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7665d48c12218c9d993e970ae28f77246d9c2ec10b8bda14d65bfadc46fdd3a4.jpg) # Analyze cell confluence and cell number in every well to verify monoclonality ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9f9e614cab9ebaf308375030245612a0cf8d9342c00ce5591738743f0d72b59a.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9dcff3d4c0a885152be06fc41ce8b168a0d49a963f2bf60ea2b650d0434ff8fb.jpg) # Verification of monoclonality — viewing the origin of a colony The growth (image) history of each well can be tracked back to its starting point—providing evidence of monoclonality. # Develop stable cell lines # Typical cell line workflow Cell line development is the process of establishing a clonally-derived cell population which has been genetically engineered to express a desirable phenotype (such as producing large amounts of recombinant protein) for a stable period of time. Single cells proliferate to form colonies that can then be assessed for the desirable characteristic. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/76843ae74e344981110bf0c8cc69b43877f3f676b2d25ce193cf8d7b5b03294b.jpg) # Accelerate cell line development with a range of Molecular Devices platforms # ClonePix 2 Mammalian Colony Picker Automatically screen more clones in less time than conventional techniques, select cells with optimal expression levels, and pick colonies with accuracy. ClonePix systems are now used in over 100 laboratories around the world to increase workflow productivity, leaving more time to better characterize target proteins and run new projects. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9f164a018911116592386928820dce45564144413cccddf58c02812185f94d79.jpg) # DispenCell™ Single-Cell Dispenser The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument developed for fast, easy and gentle single-cell isolation. Designed by scientists for scientists, DispenCell has been designed to integrate seamlessly into your lab routine, with a plug-and-play approach. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9d704bd180dec2d244ebfc4edee5d56f3cc26f44ea49b0a1fdd532f4921b6794.jpg) # CloneSelect Imager – standard and fluorescence ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/abf5233c36d3048b1bf12041c992cee898d0cbdafc49e93e5a54e240744b63e2.jpg) Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom With high quality imaging and intelligent image analysis, CloneSelect Imager allows you to assess cell confluence objectively and quantitatively. Cell growth is viewed and tracked in every well in every plate. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ©2023 Molecular Devices, LLC 2/23 1966D Printed in USA ## QPix Colony Picking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/93433f135fc49a71d59170ddc84f34da4fc1dbfc6b1a8bf3a5fd384568a33278.jpg) # QPix colony picking series High-throughput, automated colony picking and screening with data management # Colony pickers that do more ${ \\mathsf { Q P i x } } ^ { \\otimes }$ systems earned a well-deserved reputation for performance and reliability during the race to sequence the human genome with the Human Genome Project, and continues to support important areas of research. Discover why research institutes, sequencing facilities, biotech, and pharmaceutical companies worldwide use over 600 QPix systems every day. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/cc3d4b9255f342f4769625e2ed17c85148d2716a36ba3adde97d18024d3d9ce6.jpg) Get the highest level of flexibility in application and experimental design with the QPix HT System. # Key benefits of automated colony picking • Screen and pick microbial colonies • Pick 3,000 colonies per hour • Get up and running quickly with easy-to-use, application driven software interface • Future-proof your investment with a modular design that’s automation compatible • Track comprehensive data from start to finish • Do more than just colony picking—plate replication, cherry picking, plating and streaking # Accelerate objective, quantitative colony selection # Develop constructs or target proteins for protein expression more efficiently • Prescreen to pick the right colonies • Identify colonies that have desirable morphological and spectral characteristics Automate and track the entire workflow from spreading of transformed cells to colony picking and library management ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/bc2a3d19f419244bd519298b76b8ffa2c50cd9f9018cb1f8a8ea26dd67cefd6f.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3a9b1f9ebb4dcf617156c8943e203a46f00bc48f16bd1f194eb88b16d22024ce.jpg) All colonies detected in white light ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b727b231e3ee52ac51d0d7d39ef0fba04e1cdc4a88d7c3b79122c329ad75ec61.jpg) Colonies expressing required level of target protein identified in fluorescent channel (optional) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2513736a0074aae5e2f71e826416e13b6f36745413f0aaf17f95e80dfed1389f.jpg) Selected colonies ready for picking # Zymergen uses the QPix systems to make better microbes in industrial fermentation The Challenge: Zymergen is a technology company using high-throughput biology to drive the next industrial revolution. Their method of designing, building, and testing microbes rely on advanced molecular biology tools, robotic automation, and proprietary computational and analytical methods. The Solution: This capability allows Zymergen to generate novel chemicals, advanced materials, and pharmaceuticals much faster and at a lower cost than current approaches. Zymergen’s growing fleet of Molecular Devices QPix® 420 systems allows them to identify and pick large numbers of colonies quickly, accurately, and reliably. The QPix systems are a critical link in their microbe engineering pipeline, integrating seamlessly with their state-of-the-art laboratory information management system (LIMS). ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e09429b91cca9487e5dbfe9e8e64f588b95a36ec121a8f2e6387616f3f4606e3.jpg) # Key applications # For use in synthetic biology, biotechnology, biofuels, agriculture, microbiome, environmental science, food and beverage, academia, and government research # Protein engineering Us e the Q Pix system for: Colony picking Library management Protein engineering is an iterative two-step process that involves generating libraries of protein mutants and then screening them to select for desired traits. It can be used for a wide range of applications including antibody discovery, enzyme evolution, and structural biology. The QPix system can be used to increase the throughput of variants to be screened. Its accurate, high-speed picking protocols allow users to screen through a richer genetic library, increasing the chances of a desirable hit. Additionally, library management features provide robust sample tracking. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ebeddfb7fe419097c6dc111590ae34d705ffa61b88d654e01d445e6c8457d76f.jpg) # Molecular cloning Us e the Q Pix system for: Plating and streaking Colony picking Library management Molecular cloning is the assembly of DNA into a vector, leveraging E. coli as a host organism to produce plasmids. Using the QPix system to automate the laborious process of plating and streaking, as well as colony picking, frees scientists to analyze data, not perform repetitive, time-consuming tasks. Increase throughput and speed while also increasing accuracy with complete audit trail and sample tracking. # Microbial strain engineering Us e the Q Pix system for: Plating and streaking Phenotypic selection Colony picking Library management Strain engineering is a broad term that refers to the manipulation of genetic pathways to harness the power of existing biological systems in novel ways (often to manufacture molecules or proteins). The QPix system allows identification and selection of variants with desired characteristics in a high-throughput manner. Optional fluorescence imaging can be leveraged to prescreen colonies and automatically image and select clones of engineered microorganisms. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/30765c3ba10df86fcda26cbf44407553664afead6243c0c41cce3f54f1dd911a.jpg) # Gene editing/CRISPR in microbes Us e the Q Pix system for: Library management Pooled library picking Gene editing techniques, such as CRISPR-Cas9, have improved the ability to create precise mutant libraries, allowing scientists to explore the genome more accurately. The QPix system makes it possible to screen a larger library than what could otherwise be done manually. # Phage display Us e the Q Pix system for: Colony picking Library management ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/5d6fba16957b7b2da7f5c8b8ef9971270f6410a9360547206ba431b0c03c7447.jpg) Phage display is commonly used to find high affinity interactions between antibodies and antigens, which play a critical role in viral pathogenesis, vaccines, and other treatments. The QPix system can be used to increase the throughput of colonies screened, increasing the chances that you are able to identify a high affinity binder. # Plate replication and rearraying—liquid to liquid # Automate the process of creating sample banks while eliminating errors • Create duplicate and sub-libraries to maintain clone viability • Streamline downstream assays and sample management • Identical replicates (96- or 384-well) • Compression $4 \\times 9 6$ into 1x384) or expansion (1x384 into $4 \\times 9 6$ ) • Hit pick/cherry pick selected clones into new destination microplate with high accuracy ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/95592758e42bb4217b1e76b3bf61e73f74976b5b74e13b7a41395221ebdc25eb.jpg) Replicate (96x96 or 384x384) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9b27f4c5d1a485d22bcdc63c0473d050418f04fa7eb5f40568374b7fcfa78cde.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ab42e9335cc12786a4a37b510ddc2ee80d14f458db99d07f9f51aa604c46ad41.jpg) Cherry pick best clones # Inscripta enables scientists to perform digital genome editing with their Onyx system integrated in a fully automated workflow that includes the QPix system The Challenge: Inscripta’s vision is to democratize scalable genome editing to the world by offering a holistic platform consisting of software, instrument, reagents, and consumables to enable forward engineering solutions that are faster, easier, and more cost-effective for their customers. The output of the Inscripta Onyx genome editing system is a pool of cells containing up to 10,000 unique edits that necessitate picking large numbers of colonies (e.g. 1,000 – 10,000) into high-throughput microtiter plate-based screening workflows. The Solution: The QPix and Onyx systems are synergistic tools that allow customers to effectively generate and screen phenotypic diversity to enable successful bioeconomy solutions for the next generation of synthetic biology companies. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6294deab12706ad8ac06390252eb438a2f73c67b3ba7e9e8a9bfdfffc3d92787.jpg) # QPix Insights # Powered by Polar.ai # Optimize colony picking strategies with advanced AI/ML analysis Microbial screening can be labor-intensive. Our QPix Microbial Colony Pickers automate colony selection and various sample preparation and plate handling processes. But how do we analyze the large volumes of multi-dimensional data generated? With QPix Insights software, powered by Polar.ai – an AI/ML and advanced analytics & reporting tools – you can identify the best colonies aligned to optimal selection criteria with greater confidence in a shorter time. User-Friendly Interface: An intuitive interface simplifies complex data analysis, making it accessible to all levels of scientific personnel AI-Powered Precision: Reduce errors and increase reliability Streamlined Data Integration: Seamlessly integrate data from various sources for a comprehensive overview and simplified analysis process. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ee859a914942947f1e3360413740cd3fc34d95e2d6aab2687b584f7e9fdf2fac.jpg) • Cost and Time-Savings: Pick the right colony faster, leading to fewer reruns, less waste, and more time for value-adding work. Optimized Colony Selection: The software’s intelligent algorithms analyze colony parameters and analytical outputs to recommend the best selection criteria # QPix® FLEX™ Microbial Colony Picker Plating, streaking, picking, and liquid handling, all in one compact device? The QPix® FLEX™ Microbial Colony Picker makes it look easy. With advanced color imaging, it helps you spot the right colonies faster. And yes, it fits on your bench or in a hypoxic chamber – because why wouldn’t it? QPix FLEX replaces the clutter with one smart, streamlined system built to move your research forward. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/393a28ca1818daa5ea77b485c3a631c06808f0fadfeb08b8f5f231b7f3dd1a71.jpg) • High Resolution Color Camera: The QPix FLEX system classifies and selects colonies based on morphology and color – ensuring you pick the most relevant and viable colonies every time. • Hypoxic and Anaerobic Chamber-Ready: With its small footprint, the QPix FLEX system fits directly into hypoxic and anaerobic chambers, making advanced automation accessible in environments where space and sterility are critical. • Ultimate Flexibility: Switch between eco-friendly sterilizable tips and disposable plastic tips without changing hardware, giving you flexible control over sterility, sustainability, and cost. • Automate Every Step of Your Workflow: From plating and streaking to colony picking, liquid handling, and cherry picking, the QPix FLEX system automates every step in your microbial screening workflow – saving time, reducing error, and streamlining operations. • Automate Liquid Handling: Automate complex liquid handling tasks like media dispensing, glycerol addition, plating, and hit picking without needing additional instruments, thanks to the versatile 4-channel pipettor. • 2D Barcode Scanning: Maintain full traceability across every plate and pick with automated 2D barcode scanning, supporting reproducibility, regulatory compliance, and high-integrity data. # Tailored solutions for every application and workflow # Get the highest level of flexibility in application and experimental design Our expert team of engineers and application scientists can tailor the $\\mathsf { Q P i x } ^ { \\otimes }$ systems to meet your workflow demands for automated microbial colony picking and plating. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e3a7b727c351316e1babc7ddcef5aecbc1cc432121c2f8b92f85034ac11b8275.jpg) # Scalable automation Modular design allows you to future proof your system by allowing you to add additional pieces of instrumentation down the line. As your workflow changes and adapts, our systems scale with you. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b154234196f8aeeba7d88fd9029f3de6f2377840c73e46db17e64c454a503187.jpg) # Biological validation We work with you to understand your process and biological requirements so we can reproduce and test the actual performance in-house prior to shipment. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/77a097fb0e09374d3760e2db380d8a8b5af78513332539c017d5b620deb274c1.jpg) # Customized solutions Offer services from consultation through implementation. Includes throughput analysis, software and hardware customization, factory acceptance, site acceptance, and instrument validation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/cadbb7d245eddf23990703c56575070e7caa210de89d0d103d8765d543c63e0f.jpg) # Easy integration The QPix system can be integrated with other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell. We can tailor QPix systems and deploy the integration, or provide an open API and software support for the integration process. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c67ded414892f40959cee6439f4c801b56eda2653d49a117b9d99286368be16a.jpg) # QPix XE system Compact automated colony picker The QPIX XE is a compact microbial colony picker ideal for labs that have reduced space, but still need efficient colony picking throughput. The QPIX XE is a great entry level system to replace highly subjective and error prone manual picking with automated colony picking. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b97f0814cec56d70a6d083e52fd31c55bbe4a293c125b61ab9f636a24c3fb7df.jpg) # QPix 420 system Automated colony picker The QPix 420 system is ideal for groups looking to increase the throughput and efficiency of their colony screening. The system increases walk away time with the ability to pick up to 12 96-well plates before needing manual intervention, at a throughput of 3000 colonies/hour. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/5c7c90df6516b00c7a9bccc0ebf32203e3206b6cfcfc6d5ebe0ee73982b0a9c2.jpg) # QPix 450/460 systems Plating to picking Increase throughput with this QPix system, which handles up to 210 destination plates in three stacker lanes. Optional fluidics for plating and streaking allows you to plate samples, as well as pick them. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b73cf6acc39bd8a16e78ee6812d21420b2cddfb014cd4a71453953cf9223bd88.jpg) # QPix HT system # Flexible, modular, fully automated colony picking and library management system With a fully automated workflow, this QPix system provides the greatest flexibility in application and experimental design including sample plating and streaking. The system comes ready for integration with robotics for maximum throughput and walkaway time. “The QPix 450 system outclasses rival systems, handling fluorescent picking with an unrivaled level of ease and accuracy.” —Marc McCarthy, High Throughput Robotics Specialist, Alimentary Pharmabiotic Centre, University College Cork, Ireland # QPix system specifications # Instrumentation | | | | --- | --- | | Source plate capacity | QPix 420 system: Without manualintervention:1x15cm petri dish; 5×9cm petri dishes; 2 x OmniTrays;1× 22 cm QTrays | | Picking destination plate type | Various, 24-, 48-, 96-, or 384-wellincluding deep well | | Picking height | Integrated ultrasonic agar height sensor to set agar height per plate for accurate picking | | Picking head | Full pneumatic, 96 pin picking head. Interchangeable heads for other applications | | Picking pin types | Range of organism-specific pins | | Picking capacity | 3000 colonies per hour in white light, 2Ooo colonies per hour in fluorescent light | | Fluorescent picking | Colonies imaged in white light for location identification and fluorescence for data analysis. WL and FL image multiplexed. | | Fluorescence data | Multiple parameters available e.g. interior mean. Fluorescent intensity recorded for picked colonies. | | Wash bath | 3 x static wash baths | | Pin drying | Proprietary halogen pin drying station | | Dimensions | QPix 420 system (without table): 1460 mm (width)x 770 mm (depth)x 750 mm (height) | | Compressed air specifications | | Air | | | Minimum operating pressure | Clean, oil-free with sub-micron filtration 6 bar (~90psi) | | Minimum operating volume | 80L/min | | Imaging | | | | | Camera (white light only system) White light imaging | CCD Camera, image resolution: 22 pixels /mm. Field of view: 62 x 46 mm | | Camera (white light and | Trans-illumination | | fluorescence system) | CCD Camera, image resolution 22 pixels /mm. Field of view: 32 x 24 mm Epifluorescence ilumination, five standard wavelengths included: | | Fluorescent imaging (optional) | Ex/Em: 377/447 nm for DAPl /Hoechst Ex/Em: 457/536 nm for FITC/GFP Ex/Em: 531/593 nm for Cy3 /DS Red Ex/Em: 628/692 nm for Cy5 | | Colony screening parameters | Ex/Em: 531/624 nm for Rhodamine /Texas Red Colonies selectable based on size, proximity,roundness. Selection performed on whole tray image. | | Tracking | 1 × barcode reader for tracking of source and destination plates. Data tracked through allapplications for plates with same ID. | | Software Plate replication, re-arraying | | | and gridding | Software license and additional picking heads | | Zone of inhibition detection | Software license | | Colorimetric colony selection | Software licenseand filters | | Regulatory approval | | | Compliance | | | Quality | ISO9001:2008 certified | Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +1.800.266.5338 \* Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom ## Monoclonality Report Feature ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/174e9bae7f56830a3063e62341b3d08f1110ddad5baf9c267beecc403eeb2cb2.jpg) Providing image evidence of monoclonality in the celline development process is not as simple as exporting an image of a single cell.For example,high-resolution images of the entire well should also be inspected to ensure the absence of a second cell. With a few simple clicks, the Monoclonality Report feature on the CloneSelect? Imager (CSl) objectively organizes the supporting image evidence needed to establish clonality into an easily shareable report, saving researchers hours typically required to do the same process manually. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/aacae422a3ac22b5ed36b6fcddfadaeff9666e24f5d05bc906408e7a9c888ab1.jpg) Example pages from the monoclonality report. # Key features · Easily identify and select single-cell and artifact regions to include in report · Export high-resolution images of single cells,artifacts, and entire well(optional) · Automatically identify single cells with fluorescence version of CloneSelect Imager · Publish monoclonality report in PDF or Word Format # 'he reporting feature automatically generates the following data in a presentable format: # Monitorcell linedevelopmentovertime To characterizethe growth froma singlecelltoa colony,cellregions canbe designated and adjustedforeachtime point inaseries. # Monoclonal # Not Monoclonal ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2ce8283c96acbb986cc1785c17ada48d5e61eb728a91cdfef8e267dc45ed0e38.jpg) A single cell on Day O and two cells on Day 1 confirm monoclonality. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6c005b7e4086b0fde55e581b0023b50957a80af119950c54e4becdc552780798.jpg) Two cells are visible on Day O,confirming that the cell line is not monoclonal. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/1a675328ec613ffacf7016a74c1fef2d97921dba324c3f7806fe8372ae95aafa.jpg) Fourcells have developed on Day1, confirming that the cell line is not monoclonal. # View entire well at final time point Quickly determine clonality of a cell line by visually inspecting the presence of multiple colonies in a single well. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2505e26657b00d145afdb37589a8bb94b3c9949ca539262723d50e2ccf640102.jpg) # Divide anentire well into individual images Wellscaptured on Day10.The well on the left shows one colony while the well on the right shows two colonies. Export an entire well into 81 separate images to objectively confirm the absence of another cell. # Highlight regions to reviewnon-cellobjects ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/199ce98443672a0e07d270aca1cc87a34a0c755d339c2828bfd309f435fa5556.jpg) An image of a well is divided into regions, and a selected region is displayed over time. Selectivelyhighlightpartsofa well to differentiate cells from ambiguous objects. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/1ef977e7ffe21dcde1f9f533b326c2920fd7226711fb5ef5c86d7a9f9fec3b0e.jpg) A selected artifact region and its corresponding images over time isshown here. # Additional data included in report ·Report Date ·Well Name ·Date Imaged ·Region Locations ·Operator Name· $%$ Single Colony Wells ·Barcodes ·Experimental Notes # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check ourwebsite fora current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria,eirkd,d,d,lyg, Portugal, Spain,Sweden,Switzerlandand United Kingdom ## QPix XE Microbial Picker ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/05727b6f5a032f84512f735ecc04d9f1a1c61fe0e7efbedb19c56f037e416b70.jpg) # QPix XE Microbial Colony Picker With the all-new QPix™ XE, focus on discovery instead of repetitive, error-prone tasks ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/5aa712428316ff743ff7ac3bd740ddc631f678a8d5f2bc77d653fa363ada1af1.jpg) # Accuracy, precision, and quality – all in a smaller package Although smaller than other colony pickers on the market, don’t let its size fool you – it doesn’t compromise on results. The QPix XE streamlines your colony picking process, allowing you to focus on discovery and not repetitive, error-prone tasks. # So why not choose the smarter option? Choose the QPix XE Microbial Colony Picker today and see the difference it can make in your research. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a47e406a6b90285fabe54875672615e77d3b66142f1d34d6e7840cf59223d618.jpg) Modular and scalable design for future throughput growth or walk-away time ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ad9ccf50ccc4ef51014712e2991d879d7e4330a2bc31ae1f7ebf854ef537df11.jpg) Easy to use, intuitive, process- driven software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/195d5d5fce50f47fb08bfcad34f2a2d23f6e035676817b66e8f856982451d4db.jpg) Compact size saves high-value lab space without compromising efficiency ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6291e269e3bccfcf8cf86d6e43a6510d394371fd4cab5547da95215fe9ef75ad.jpg) High picking efficiency of 1500 colonies per hour ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/f71677c6f7e07bf5d0b8df23561fa167fb00cebfb235294df97ee083958d332d.jpg) Screen and pick single colonies based on morphological characterization ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/cd1418a5a1cd4feed25bad337b7197f45df674b87de3fabd8ab6ccceb1fd27bd.jpg) Automatic sample tracking and data storage # Key benefits of automated colony picking • Screen and pick microbial colonies • Pick 1,500 colonies per hour • Get up and running quickly with easy-to-use, application driven software interface • Future-proof your investment with a modular design that’s automation compatible • Track comprehensive data from start to finish • Do more than just colony picking—plate replication and cherry picking # Key applications For use in synthetic biology, biotechnology, biofuels, agriculture, microbiome, environmental science, food and beverage, academia, and government research # Protein engineering # Molecular cloning # Colony picking \| Library management Protein engineering is an iterative two-step process that involves generating libraries of protein mutants and then screening them to select for desired traits. It can be used for a wide range of applications including antibody discovery, enzyme evolution, and structural biology. The QPix XE can be used to increase the throughput of variants to be screened. Its accurate, high-speed picking protocols allow users to screen through a richer genetic library, increasing the chances of a desirable hit. Additionally, library management features provide robust sample tracking. # Colony picking \| Library management Molecular cloning is the assembly of DNA into a vector, leveraging E. coli as a host organism to produce plasmids. Using the QPix $\\mathsf { \\times E }$ to automate the laborious process of colony picking frees scientists to analyze data, not perform repetitive, time-consuming tasks. Increase throughput and speed while also increasing accuracy with complete audit trail and sample tracking. # Microbial strain engineering Phenotypic selection \| Colony picking Library management Strain engineering is a broad term that refers to the manipulation of genetic pathways to harness the power of existing biological systems in novel ways (often to manufacture molecules or proteins). The QPix XE allows identification and selection of variants with desired characteristics in a high-throughput manner. Optional fluorescence imaging can be leveraged to prescreen colonies and automatically image and select clones of engineered microorganisms. # Gene editing/CRISPR in microbes Library management \| Pooled library picking Gene editing techniques, such as CRISPR-Cas9, have improved the ability to create precise mutant libraries, allowing scientists to explore the genome more accurately. The QPix XE makes it possible to screen a larger library than what could otherwise be done manually. # Phage display # Colony picking \| Library management Phage display is commonly used to find high affinity interactions between antibodies and antigens, which play a critical role in viral pathogenesis, vaccines, and other treatments. The ${ \\mathsf { Q P i x } }$ XE can be used to increase the throughput of colonies screened, increasing the chances that you are able to identify a high affinity binder. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/58e92ab76da014c3ed1e211eaa4c8eb6b4ff6931bc2b279793c4510f4529e76c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/22234ddbfc6b5ba193a9a63e766951252ae13bea8f96d8bdc6930988bf7ebafd.jpg) # QPix XE system specifications # Processes and software licenses Instrumentation | | | | | | --- | --- | --- | --- | | Model | 110V / 220V | Colorimetric colony selection (Blue/ | Software license and fiters | | Dimensions | | white colonies) Controlplate | | | Gas requirement | | Software license | | Type Specification | Compressed air Clean oil-free submicron filtration | Picking Processes | | Minimum operating | 6 bars (~90 psi) | Picking head | | | pressure Minimum operating | | Picking height | Integrated ultrasonic agar height sensor to set agar height per plate for accurate picking | | volume Accessories | 80L/min | Picking pin types | Range of organism-specific pins | | | | Picking throughput | 1500 colonies/hr | | Picking heads | i | Sourcepate | Wtpee | | Picking pins | Range of organism specific picking pins (optional) | Source/picking plate compatibility | · Omnitrays & divided Omnitrays · QTrays & divided QTrays | | Imaging | | ·Petri Dishes | | White light | · Epifluorescence with five standard wavelengths | Destinatio plate | Pi | | WL+ fluorescence modules (optional) Camera | · Ex /Em: 377 /447 nm for DAPl/Hoechst ·Ex /Em: 475 /536 nm for FITC/GFP ·Ex /Em: 531/593 nm for Cy3/DS Red · Ex /Em: 628 /692 nm for Cy5 · Ex /Em: 531/624 nm for Rhodamine/Texas Red | Destination plate compatibility | (including both source and destination) ·96/384 well plates (Standard) | | · Deep well plates · 24 well plates (Not Standard) | | ·Ability to change plate types Ce | | White Light only | · CCD camera · Image resolution: 22 pixels/mm | Fiutreset gicking Data | WL and FL multiplexed | | White light and | · Field of View: 62x46mm · CCD Camera | Colony screning (WL/White light) | Colonies selectable based on size, proximity, roundness. Selection performed on whole tray images | | Fluorescence · Image resolution:22 pixels/mm | | modules Sterilization | · Field of View: 32X24mm | Colony screening parameters (FL/Fluorescence) | Multiple parameters available e.g., interior mean. | | Wash bath | 3X static wash baths | | Fluorescent intensity recorded for picked colonies. 1x barcode reader for tracking of source and destination | | Pin drying | Proprietary halogen pin drying station | Tracking Automation | plates. Data tracked through all applications for plates with same ID | | Processes and software licenses | | | Picking Standard | Software license | Regulatory approval | Available as Custom Solution | | Regional picking | Software license and additional head | Compliance | CE | | Pltrearlaiann | | | | Quality | ISO90001: | | Zone finhibtion | Software license | | Custom solutions are subject to Molecular Devices Custom Products Purchase Terms | | Contact Us | Regional Offices | available at http://www.moleculardevices.com/custom-products-purchase-terms | | | Phone: +1.800.635.5577 | USA and Canada +1.800.635.5577 | Taiwan/Hong Kong +886.2.2656.7585 | | | Web: www.moleculardevices.com | United Kingdom +44.118.944.8000 | Japan | +81.3.6362.9109 | | Email: info@moldev.com | Europe\* 00800.665.32860 | South Korea | +82.2.3471.9531 | | Check our website for a current | China +86.4008203586 | India | +91.73.8661.1198 | | listing of worldwide distributors. | usti | | | ## Advanced Workflow Solutions Advanced Workflow Engineering Solutions Tailor our instruments. Transform your research. www.moleculardevices.com 2 BENEFITS • Purpose-built solutions for your specific application need • Tested for your biological applications • Shorter development cycle for novel applications • Global service and support – from consultation and feasibility testing through implementation of your solution When standard doesn’t fit, customize our instruments — not your research. Advanced Workflow Engineering Solutions (AWES) is the Molecular Devices service team of in-house experts who can skillfully customize our instruments to optimize workflows for your specific needs. Our Advanced Workflow Engineering Solutions (AWES) team provides: • Comprehensive package of services from consultation through implementation •Complete setup and support for your fully integrated workflow •Validation testing with actual biological samples •In-house expertise for both application needs and instrument design • Global service and support with over 30 years of experience in life science research Advanced Workflow Engineering Solutions 3 QPix™ Microbial Colony Pickers & ClonePix 2 Mammalian Colony Pickers • Hardware customization – from custom labware to unique transfer pins to enhanced optical systems • Software customization – modify software to match specific API needs, enable unique workflow capability, integrate into LIMS/ data management systems, customize UI, control custom designed hardware or modify imaging analysis criteria • Biological validation – we’ll reproduce and test in-house with actual biological samples The Molecular Devices Advanced Workflow Engineering Solution (AWES) team has successfully tailored QPix and ClonePix 2 colony pickers for some customers to integrate with other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell. Microplate Readers • SoftMax® Pro Software customization – customize data transfer import/export, customize for GXP , create unique protocols, and much more • Hardware customization – plate loading and special plate handling requirements, custom cartridges The Molecular Devices Advanced Workflow Engineering Solution (AWES) team has successfully tailored microplate readers for some customers to customize software capabilities and to integrate with other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell. ImageXpress® High-Content & Cellular Imaging Systems • Software customization – modify software to match specific API needs, enable unique workflow capability, integrate into LIMS/data management systems, customize UI, control custom designed hardware • Biological validation – we’ll reproduce and test in-house with actual biological samples The Molecular Devices Advanced Workflow Engineering Solution (AWES) team has successfully tailored the ImageXpress Micro system for some customers to include a variety of light engines to address ultra violet (UV) to near infrared (NIR) applications, environmental control with gas mixers for CO 2 and Hypoxia, fluidics with simultaneous imaging and dispensing as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell. CloneSelect™ Imager • Software customization – leverage fluorescence imaging to automatically identify single cells • Hardware customization – modify hardware to enable fluorescence imaging capabilities and to handle custom labware The Molecular Devices Advanced Workflow Engineering Solutions (AWES) team has successfully tailored the CloneSelect Imager for some customers to include fluorescent detection for expanding monoclonality verification and cell-based assay workflow options. Solutions provided by AWES are subject to Molecular Devices Custom Products Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms. Explore modifications with AWES The AWES team is available to you to explore these modifications. Price, time to deliver, and specifications will vary based upon mutually agreed requirements. Solution requirements may cause adjustment to standard performance. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ©2018 Molecular Devices, LLC 1/18 2168A Printed in USA Regional Offices USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.2248.6000 Japan (Osaka) +81.6.7174.8331 Japan (Tokyo) +81.3.6362.5260 South Korea +82.2.3471.9531 Contact Us Phone: +1.800.635.5577 Web: www.moleculardevices.com Email: info@moldev.com Check our website for a current listing of worldwide distributors. \\* Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland Turnkey service Our AWES team works with you to understand your needs and challenges. We share our application and instrumentation expertise and propose solutions. We then test the chosen solution in-house to ensure it works for your application. Upon validation, we install the system in your lab and follow through onsite to support you as you advance your research. Scale-up automation We adapt your system to seamlessly integrate into larger automation workstations. AWES can design and test the whole workstation from scratch, incorporate it into an existing automated solution, or deliver a customized, automation-ready system for future integration. Hardware customization From custom labware to unique transfer pins to special handling requirements, we’ll work to find a solution and prove it through validation. Software customization We can modify the software to match specific Application Programming Interface (API) needs, enable unique workflow capability, integrate into LIMS or data management systems, customize a User Interface (UI), and of course control any custom designed hardware. Biological validation Our application scientists work with you to understand your process and biological requirements, so that we can reproduce and test the actual performance in-house prior to shipment. Global service and support With over 30 years of experience in the life science industry, you can count on Molecular Devices to deliver quality products and worldwide support. Solutions provided by AWES are subject to Molecular Devices Custom Products Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms. ## DispenCell Single-Cell Dispenser ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ad08886a9cc2d0456bb2ea8888e42927a5a7bec3e061cb95220032d764ac3fc5.jpg) # DispenCell Single-Cell Dispenser Immediate and traceable proof of clonality Empowers scientists to isolate single cells more efficiently and reliably. # Fast, easy, and gentle single-cell isolation ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/eefccc23189229bb840c1cd4f295956128ebaace24d67e9c232a791815f3d727.jpg) # DispenCell # A simple single-cell dispenser DispenCell™ is an automated laboratory instrument developed for fast, easy and gentle single-cell isolation. Designed by scientists for scientists, DispenCell has been designed to integrate seamlessly into your lab routine, with a plug-and-play approach. # Traceability # Single-cell mapping Single-cell analysis software provides a map for immediate and traceable proof of clonality. DispenCell’s single-cell dispensing unit is fitted with a sensing tip that detects the passage of the cells. As each cell advances, a unique electrical signal is triggered. This unique electrical trace is immediately recorded, allowing the user to check for proof of clonality immediately after the cells are dispensed. The full set of data is stored in a proof of clonality report. DispenCell’s technology is patented. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/36a2c12183c22dd59f6d46005bbed60cad5d1c163ee00a1a6f098766f24bef0f.jpg) | | | | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | | SEED·Biosciences | | PlateN°02 96wells Results Data | | | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 10 | | A | | √ | √ | | √ | | √ | √ | | | B | √ | √ | √ | √ | √ | √ | | X | √ | | C | L | × | | | X | √ | √ | √ √ | √ | | D | √ | | | √ | √ | | √ | X √ | √ | | E | √ | | | | | X | √ | X √ | | | F | √ | √ | | | √ | √ | √ | √ | | | G | X | √ | | | | | √ | √ | X | | H | | | | | | | | √ | X | | Singlecell | XDiscarded well | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/0ca0159c78c10c48d2a66d7c8b7fc970f68d899e27fbfa9134240bfa8f91918d.jpg) # DispenCell is fitted with a sensing tip that acts as a Coulter counter As a single cell passes through the Coulter aperture to flow into the well, it leaves an electrical signature that appears as a unique peak, whereas multiple peaks result from doublets or multiple cells. # Benefits # An enhanced user experience for faster and better results ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7ebe6ec70eabc93489ee8fd974df75fec0058644faae22654070fa8eab34831b.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/de035039d0b67ca60f96d8329d0dd064b32efff6c0ee059bbbd17182bc726d3f.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a93a4da5aaef57f713f72d24578fa0fe8247a29fffdd4a18e3c481ada327798a.jpg) # Easy to use Intuitive with a simple interface and easy to set up. No cleaning or calibration required. # Contamination-free A patented disposable tip ensures clean isolation of single cells and no crosscontamination. Certified free from animal products and cytotoxic material. # Proof of clonality A single-cell analysis software tool provides a traceable proof of clonality report instantly. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/46209cc03268208d040c4d458d188e95352a29a0fa29d1c5ee692f812483bdfc.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/cdaf37a61f313189a4822846d1001b7de73269113369218c23a76f0550641fc1.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6b1ae33c75d62f0b68c7843aa8c46464d721fb1ee2968f2e88d3d4e28968cf08.jpg) # High cloning efficiency Its unique design ensures gentle dispensing for better viability and cloning efficiency. # Compact Benchtop-sized, DispenCell fits perfectly under a hood to work under sterile conditions, on a bench top for routine seeding or in a pre-existing automated workflow. # Gentle Extremely gentle handling of cell samples, comparable to manual pipetting (less than 0.1 psi), preserving cell viability and outgrowth. # Suitable applications # The right instrument to optimize your workflow Single-cell isolation and proof of clonality are essential to multiple applications, including cell line development, CRISPR-mediated gene editing, rare cell isolation, monoclonal antibodies screening and single-cell genomics. Application notes are available to guarantee an optimal user experience. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ff6d152b5543499140a116ffc6929a9e933d96749ae5581d52c7b305e10711a1.jpg) “ The DispenCell is not rocket science and that’s exactly why we like it. User adoption is easy, it is gentle with cells and it provides monoclonality assurance. Those are key features that we need in a robust iPSC line development factory.” —HANS WEBER Automation Lead, Century Therapeutics # Cell-friendliness # As gentle as manual pipetting DispenCell’s unique technology allows extremely gentle handling of the cell sample, comparable to manual pipetting (less than 0.1 psi), yet more efficient. Consequently, cell viability and outgrowth are preserved. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/36162c1baec13f242afe605d4bdd3eb7433305c42c6cea4092e7fb789f4e05ed.jpg) Clonal outgrowth of a single CHO cell dispensed with DispenCell. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e58d9cddd59841dab8501c13d20dd5b7aaf6bc62669bc054292ea2d115611525.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a4ac57cca13f7c9bc0e38e0a97c9fbc6c0d27a59be2629d3094817e4119ccdc8.jpg) # Technical specifications # DispenCell | | | | --- | --- | | Pressure | Less than O.1 psi | | Plate holder | 2 plates (96 or 384 wells) | | Minimal cell number | 100 cells | | Calibration | No need | | Cleaning | No need | | Sample prep. | 10 min | | Dispensing | 96 well plate in 5 min | | Cell parameters | Size, doublets | # References Bonzon, D. et al. (2020) ‘Impedance-Based Single-Cell Pipetting’, SLAS TECHNOLOGY: Translating Life Sciences Innovation, 25(3), pp. 222–233. doi: 10.1177/2472630320911636. Muller, G. et al. (2020) ‘Traceable Impedance-Based Dispensing and Cloning of Living Single Cells’, SLAS TECHNOLOGY: Translating Life Science nnovation, $2 5 ( 3 )$ , pp. 215–221. doi: 10.1177/2472630320905574. Hannart, H. et al. (2021) ‘Traceable Impedance-based single cell pipetting: from a research set-up to a robust and fast automated robot’, SLAS TECHNOLOGY: Translating Life Sciences Innovation. doi: [https://doi.org/10.1016/j.slast.2021.12.003](https://doi.org/10.1016/j.slast.2021.12.003) Ben Khelil, M et al. (2021) ‘A new workflow combining magnetic cell separation and impedance-based cell dispensing for gentle, simple and reliable cloning of specific ${ \\mathsf { C D 8 } } +$ T cells’, SLAS TECHNOLOGY: Translating Life Sciences Innovation. doi: [https://doi.org/10.1016/j.slast.2021.11.001](https://doi.org/10.1016/j.slast.2021.11.001) # Verify monoclonality confidently at day zero with CloneSelect Imager FL and DispenCell bundle ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/92f1eebad043c8f46d6b58a14eaafe3846ac636503192ba248bc1505811326f6.jpg) # Streamline your workflow when you pair the DispenCell with the CloneSelect Imager FL The all-new CloneSelect Imager FL adds high contrast multichannel fluorescent technology in addition to the standard white light imaging that allows for accurate singlecell detection and proof of monoclonality at day 0. Identify and verify gene edits with comparative confluence assays. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/348ce074e7ee0d93a3ce4f9ab37ec69cc0fd7c0e516802514c0339d3cd1ac4c6.jpg) \*Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom • Document evidence of single cells and confluency digitally for auditing and submission to regulatory authorities • Image cells non invasively at multiple time points to monitor colony formation Screen using high resolution white light imaging • Deliver real-time results with on-the-fly analysis • Automation and integration ready # Get started. Explore single-cell dispensing with us. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## Clone Screening Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/19a5edebbaa7405088993fcb6cfe63fab552ba2deba85e11c841d178caa7a886.jpg) # Clone screening solutions Advance your microbial and mammalian clone screening with proven, automated technologies # QPix 400 Series Microbial Colony Pickers Automated microbial clone screening and library management system ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c68d405097eb4467295206dc04e3f2fcb64b318baef764c4fa799490053c6048.jpg) Microbial screening colony picker with sample tracking and plate handling. Fully automate synthetic biology workflows for DNA assembly, antibody discovery and protein engineering. The ${ \\mathsf { Q P i x } } ^ { \\otimes }$ 400 Series Microbial Colony Pickers combine intelligent image analysis with precise automation for fast and efficient screening of large libraries. With a variety of data tracking and assay tools, the QPix Software streamlines the control and management of complex and iterative processes. • Use the QPix system for synthetic biology, DNA assembly, antibody discovery, protein engineering, and phage display workflows • Streamline your workflow with scalable automation – pick up to 30,000 colonies per day • Electronic data tracking for well-documented data control • Sterile environment with customizable HEPA filtration options ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/411b8ab13e9875f358ffac338bbeebc3e5d8ebad9e211c1862ecb47ba456dcd6.jpg) Build more, screen more, increase your colony picking capabilities # Key features and benefits # Identify colonies with a desired phenotype # Select colonies efficiently ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/320cd570bb75589904a745dc54a0008adcd4337ef5e498fd8016c9623b76a60e.jpg) # Maintain sterility and eliminate cross contamination ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e34c68357e94e7ba644681b1b1169969d3b5c123e3561e73e0c70beab33dcb24.jpg) The QPix colony pickers support a wide variety of microorganisms and multiple selection modalities including fluorescence intensity, blue/white selection, size and proximity, and zone of inhibition. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9d6e15fbba8658f975b359f4c4751133771042e1bcc464ddb3c984313ebdfcfd.jpg) A suite of organism-specific pin and agar sensor ensure efficient picking. The system delivers a picking efficiency of >98%, allowing you to walkaway with confidence. A host of sterility features are available including a UV light for sanitizing the interior of the instrument, customizable pin washing, and halogen pin drying. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/1f961b30ff323ea84b186a6971747da09c8d73d2cc7392e8e68651fd9d61511c.jpg) Plating and spreading ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/8b305ac3792b37f21553720a095fb8c9c0bc386589e489d62c8a80d26137b1ce.jpg) Replication and hit picking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/712c6b4b85c83821fe011e9e30ae08573d60018638a91db336fdfc119a643212.jpg) Multiple imaging modes ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7741856ce520cc9685352fb009cbf1583f0cd50d370756e86f758e850975f98e.jpg) Sustainable components ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3da92d4bea17d6094dd37b5a0d135a6ccd9d3249a3aec96175b4e0d0cc5d22aa.jpg) Scalable automation options\* ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3df1522b1150c11d8c3d535b36cc779c165682c454ccab2f6287128fee3fec1e.jpg) Organism-specific pins Price, time to deliver and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b9c729ff372cafc4b46d3a93ef4a2b93828133f28901a9b5ba1ab250bb74b333.jpg) # Molecular cloning Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector—a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. This is a very laborious process when done manually requiring hundreds of plates and kits. There are several areas where errors or contamination can occur. Automating the process can dramatically reduce labor while increasing throughput and efficiency twofold. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/572065a6165851f7b3220b6d0c84cb0cb2eab67620ed104b99212def02de0326.jpg) # ClonePix 2 Mammalian Colony Picker Cell line development solutions with automated clone screening # Automate antibody discovery and cell line development workflows Screen more clones in less time with monoclonal verification on day zero, then screen and identify for highest producers in weeks, not months. • Screen 10X more clones than limiting dilution • Increase probability of identifying high-value clones • Condense the workflow into a single solution • Eliminate or recover unstable clones early ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e6b7ad8d161aeed3e4c543cfcd48db5440b1b6846dfc188ece7b7771847b9134.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/52c8c1ee7aae32f3f1c17a4c825c4c1d0cb357394cac95f1313dce0673bd2700.jpg) # Reduce cost by finding your highest producers with fewer reagents This image represents the number of plates needed to screen 10,000 clones via limiting dilution (the boxes he’s sitting on) vs. the ClonePix® 2 Mammalian Colony Picker (plates he is holding in his hand). How many plate does it take to screen 10,000 clones? • ClonePix system: 10 plates • Limiting dilution: 1,000 plates ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9d6644534c51145fcdc694f32897344a87bfceb22cb87c918917fd81233292d9.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7349b0eadab479f0dc5de84684788010b8600b13322fbfd5e001b0a8a67638b9.jpg) Day 0 # Monoclonal # Day of picking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/7329002a6b9fe105b748bfcb646b9e858dac603b6b4cc8e26b3a7f16d61eebec.jpg) Certainty score = 0.92 # Not monoclonal ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a971ffff5ddac4f1088dfdee7e7621e7d8ca44a0bd0a0c07fac0b4ce3bac2fa2.jpg) Automatically associates highvalue colonies to day 0 image data Colonies are assessed for productivity in situ using fluorescence ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ea85811ad431ff3587809bae6a871baa21e3f6e2282eba7e66aa200e0d32818f.jpg) Certainty score $\\mathbf { \\Sigma } = \\mathbf { \\Sigma }$ 0.11 Certainty score $= 0 . 0 6$ ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a59fb28a2e23fad642434c223801bc70717416bfa5a24d81e7a6def5254f204b.jpg) Outline of colony is compared to day 0 image The enhanced ClonePix system links high-value colonies with its day 0 image for automated assessment of single-cell derived clones. Fusion software assigns a certainty score to each colony based on picking day, colony location, and single cell Z-stack images at day 0. The threshold of determining monoclonality from the certainty score is variable based on user-defined inputs. # Accelerate cell line development timelines by integrating multiple steps into a single step # Key features and benefits # Select cells with desirable attributes ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3971ae3a79f99841c8a700297f3c891a156ed41246e24cc71ba579746b78f741.jpg) # Pick colonies with accuracy ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/81e877ac53fea365a32191e49c2d81a08b1dfc41a20463ed2a276313fa03619a.jpg) Screen more clones in less time ClonePix 2 system is 10X faster than labor-intensive limiting dilution and FACS. Our sophisticated software and integrated robotics enable a screening speed of > 10,000 clones per day. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/31821e18b7e52e54a2599be9a0c1beef507c41dffe0269cb0422cd04eb814a63.jpg) Easily screen and select clones based on protein productivity, antigen-specificity, cell viability, and expression levels of tagged recombinant proteins. Picking accuracy < 1 mm. Robotic picking reduces risk of colony disturbance. Images of picked clones are stored with data. Automated plate handling ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/66d4240ecc462aca1ea5237e92c6a0c9c3aab418758af2e7207838fb1c9712d7.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/03d4f09bd03a91e2a0e4c3ee7d69a9dcd5dba9d0aa0066196dea6c9e46e1c5a0.jpg) Multiple detection methods ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c6f6843bddbc8599741a5888a123f604d22ae225ebaf433a55ff5d927465139e.jpg) Custom automation options\* ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fe8fdd2254cddf31b63da700790d1463be936930f577bc9ff357e8b3d28fb283.jpg) Maintain sterility and eliminate cross contamination # CloneSelect Imager FL High-speed fluorescence and white light imaging, intelligent data analysis and monoclonality report generation # Your next-generation fluorescence imaging solution ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/35f35f077a750a369ae7523a66b235016e89851878aa52bcd8bbb794f04d87df.jpg) The CloneSelect® Imager FL is a high throughput imaging solution that can help you track the formation of a colony from a single cell effortlessly through barcoded plates tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results. # Streamlined workflow • Demonstrated IND success. Monoclonality Reports are automatically generated based on parameters you select. • Multichannel fluorescence for evaluation of GFP/RFP expression system and day zero assurance of monoclonality. • Multichannel confluence and growth rate measurements for further cell characterization. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/70eed3c2d9461b009cb455ac1303bc66d6572d1c852ff86325e6506a49a56ff5.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d178c6c03d2157e5717816b91e7e8276054140c7c1b85cee2a87b022e7ca22f5.jpg) # Image High-speed, high resolution multichannel fluorescent and white light imaging • Optimize clonal outgrowth—particularly useful for optimizing clonal outgrowth strategies when platform approaches are not suitable e.g. when investigating new cell lines or variants. • Diverse cell types—Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/92f53c06453db65e99d9d16b2a7449660d1f79e10283d065acc89cd8f42db648.jpg) # Multichannel fluorescent imaging ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2b8121845abcf70429cbf1722c0c293ac5ec42acc03545f742c6a53ea0bad04e.jpg) Identify engineered cells with multichannel fluorescence CRISPR or other genetic editing assays Selection markers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/68685112869b5150fd67b4471d45e87c0c849870f8aba30e55888a5aaca6307e.jpg) Run hit picking assays with multichannel fluorescence Screen for productivity ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/4a9c85798502f7ffdbe4e5ae10823f48cdea00fa6b510af1fd4440f874ba1da8.jpg) Run comparative confluence assays Red vs green fluorescent channels ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/213e5079f7d5019bd03bdf03d1f30436b08bdff421cfaf59aed11008ab913997.jpg) Run cytotoxicity assays with fluorescence or white light Compare growth rates, colony size increase/decrease well to well • Track changes in cell density in response to various cellular manipulations or treatments to calculate dose response curves and IC50 Label free detection, no staining required. Avoid costly colorimetric kits # CloneSelect Imager Objective, quantitative assessment of cell growth ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/dbc783514aff980ad460e38caacbcbd29a8ead1fd0e8056df501070228bee287.jpg) # Verify monoclonality confidently The CloneSelect® Imager can help you meet regulatory demands of single cell verification with its automated analysis of cells in the white light channel. The system also enables concurrent confluence and monoclonality studies. • Document evidence of single cells and confluency digitally for auditing and submission to regulatory authorities • Image cells non invasively at multiple time points to monitor colony formation • Screen using high resolution white light imaging • Deliver real-time results with on-the-fly analysis • Automation and integration ready “CloneSelect Imager has become an essential system for verification of monoclonality within our cell line development workflow.” Dr. Howard Clarke Senior Staff Scientist in Process Development, CMC ICOS Biologics Inc., USA ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ce0ebf0a096daa1211dcfadbcf3b8662fd7893584bcc7a0e4e52c31bcbcd99a7.jpg) # Analyze # Report • Cell confluence and cell number estimation displayed for each well • Growth curves calculated and displayed ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/54ab737734f3bffd707d7c17adb17cce75de794ef6d53286d6fc60f468bebe40.jpg) • Make confident, image-driven decisions throughout plate history • Track and view growth of every cell line ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6d9e3749b07eeafec964617add2244a90ba2291c1f2d422a6d3095c231eb6036.jpg) # Growth curves calculated and displayed Electronically track and store plate data: cell confluence, cell number estimation, and growth curve ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2fdcbb44802c5d65d2e8ea323fd3eedbee874d36df0b2424649c476191d629be.jpg) 3% Day 1 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ec0003bd63a65c855dbb7c131f6ba8387a4e84d3d827dc1c5a450f3b55af6a84.jpg) 5% Day 2 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6faa6ee1bddadf9332463ea00759ab1f71ce87ec12b958a7e44751aeb80fdd25.jpg) 15% Day 3 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/958c63eade81fe1f4778be13f6f42143776acc61ff85c8bde1af1edf683ed8b7.jpg) 55% Day 7 # View every growth curve in every well # Key features and benefits ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e866a8bc7dd180a7ea757b37e28800045b5b47d152d488b1163ba33ed795903c.jpg) # Verify monoclonality easily The Monoclonality Report feature streamlines the creation of supporting documentation for regulatory agencies. Reports are automatically generated based on parameters you select. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/55180fde8b5f93a854584d5eb1fc9da3c7dd1771e7389cf26cd1b2140952162c.jpg) # Detect cells accurately Algorithms are optimized for accurate cell detection and address varying cell types and conditions. High resolution imaging provides accuracy and assurance of monoclonality. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/89e6e61194604749951901b033cac27e516ca4e0f7b0a1db6a923dc2165c132d.jpg) # Screen more clones in less time The imager delivers industry leading acquisition times, allowing for imaging a 96-well plate in as little at 90 seconds. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/1bd29658011138996c7b6f167d05c0f4b00ebc4c7780ead6df65b25e3876b450.jpg) Variety of plate formats and cell types ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/f7d0ec77dbbb36799fbde42ed1b1272dcf55e79fdd81e0f6188769d22651f029.jpg) Choice of imaging modes ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/078fc302ea00d72f119c4bd3ee0cd44a724f017c4cb24aae2b60e89d32a9156b.jpg) Autogenerated data and tools ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e05f6a134e22e3b1a3b26bcdcf6d374967d57faadf6afc44d48f5bf05bb17b69.jpg) Clear, crisp images ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/09d28a5a99a59c489c4b29b0c5d4e759edc4bc07af3a398cdafb40f50cc1f751.jpg) Intelligent analysis ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/585517b284d58a8b49a0632553bd4cb7e40d184a767487ed2407e7ac9d25b38f.jpg) Custom automation options\* # DispenCell Single-Cell Dispenser Single-cell cloning using image-based cell sorting # Automation for fast, easy and gentle single-cell isolation DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b1c419f478362ce0aca9db68f6b88778a52cb14303074777bcc5529dbf7bb87b.jpg) • Confidently isolate single cells and provide consistent, high-quality evidence of monoclonality to the FDA • Highly efficient single-cell sorting with an average monoclonality reliability of $> 9 0 %$ observed • Extremely gentle handling of the cell sample (less than 0.1 si), while preserving cell viability and outgrowth • Minimize cross contamination through disposable cell dispensing cartridges • Fast turnaround time—prints a 96-well plate in less than 10 minutes (384-well plate in less than 40 minutes) • Sort wide range of cell lines: CHO, HEK, SF9, iPSC, primary cells • Compact footprint takes up a small amount of valuable bench space # Proof of monoclonality DispenSoft, the single-cell analysis software included with DispenCell, provides immediate and traceable proof of clonality. The instrument is fitted with a sensing tip that detects the passage of cells, allowing users to check for proof of clonality immediately after the cells are dispensed. All dispensing data is automatically stored, allowing for easy generation of a clonality report. # Key features and benefits # Proof of clonality # High cloning efficiency ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/5aae5a406bb48cec632125d8bd4b7b552d0abeb1db63a67ad2315fb5c189e0eb.jpg) # Contamination-free ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/b574cbc3bb0002b8d4b839500635170ae7fbf559c3015b4ab9c3cf501f8feca7.jpg) A single-cell analysis software tool provides a traceable proof of clonality report instantly. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/5487033ea7074bfee4a54a430c183ddc986c9821ce2f0aad20bb19fa2f4f5ec7.jpg) Unique design ensures gentle dispensing for better viability and cloning efficiency. A patented disposable tip ensures clean isolation of single cells and no cross contamination. Certified free from animal products and cytotoxic material. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/be480833500cdc473da344fb87ff0bb83ac8cc530d51737f76ce110b0a69bd7d.jpg) # As gentle as manual pipetting DispenCell has unique technology which allows for extremely gentle handling of the cell sample. Cells are exposed to no more pressure than with manual pipetting (less than 0.1 psi). This allows the instrument to preserve cell viability and outgrowth while simultaneously increasing cell deposition efficiency. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6290b238a158c34be94dc5f08511eb710c06540013be0e3bf1988f877203a6bb.jpg) # Compact The small footprint of the DispenCell allows it to fit perfectly into any laboratory setting: under a hood to work under sterile conditions, on a bench top for routine seeding, or in a preexisting automated workflow. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/9f2c1e11d628851be9b21f0cb162cb75776fa93b2eed81b2510db2b4c0d597a9.jpg) # Easy to use Easy to set up, and intuitive with a simple interface. No cleaning or calibration required. # Clone screening systems # Dedicated solutions for clone screening and single cell isolation # Verify monoclonality easily Objective selection, imaging, and data collection streamlines tracking of colony formation from a single cell. # Sort viable single cells efficiently High accuracy robotics combined with gentle fluidics-based systems establish viable clones with much higher efficiency. # Streamline workflows Automation with sample tracking increases throughput, allows for more walk-away time, and provides consistent results. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/f41d6046f4d77c70a1b04928feee7435f7dcc83ec98d958bcb1230c57557536c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fd9f79d65bfccf7bb87e83d563f352b5b3c773f76e68fcccebec216e8e4b0ab5.jpg) # Develop stable cell lines Typical cell line workflow Cell line development is the process of establishing a clonally-derived cell population which has been genetically engineered to express a desirable phenotype (such as producing large amounts of recombinant protein) for a stable period of time. Single cells proliferate to form colonies that can then be assessed for the desirable characteristic. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/33fd0f53f600a7a634bfe9e30e77dc828c9c9e022e1fbf19596a1e5c69ced62b.jpg) \*Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## CloneSelect Imager System CloneSelect Imager CloneSelect Imager FL High-speed fluorescence and white light imaging, intelligent data analysis, and monoclonality report generation Scan every well in every plate Key benefits • Assess cell confluence objectively and quantitatively • Streamline workflow: image, analyze, report • Image every well anytime to track colony formation and verify monoclonality A label-free imaging solution for the assurance of monoclonality and automated confluence across diverse cell types CloneSelect® Imager estimates cell confluence and cell number. •Automatically scans every well in every plate •Generates growth curves for each well CloneSelect Imager: objective, automatic Quantitative, whole well cell confluence for every well Conventional technique: subjective, time-consuming Inconsistent results: cannot determine whole well confluence – well after well Sample by eye within wells across each plate •Enables viewing and tracking of every well in every plate • Reveals additional information on cellular morphology and an understanding of growth characteristics CloneSelect Imager FL White lightGreen fluorescent protein (GFP) Red fluorescent protein (RFP) CloneSelect Imager White light Streamlined workflow ImageAnalyzeReport 123 1 Image Seed one cell per well and image at any point. • Optimize clonal outgrowth—The system is particularly useful for optimizing clonal outgrowth strategies when platform approaches are not suitable, e.g. when investigating new cell lines or variants • Diverse cell types—Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types “CloneSelect Imager has become an essential system for verification of monoclonality within our cell line development workflow.” —Dr. Howard Clarke, Senior Staff Scientist in Process Development, CMC ICOS Biologics Inc., USA 1 2Analyze Track colony growth over time. • Fluorescence applications A 01 02 03 04 05 06 B C D E F G H A 01 02 03 04 05 06 B C D E F G H 363831291718 433929301919 433836311718 443929261717 443525271717 444025251516 483829271716 453425261617 B1: 24390B1: 43% A 01 02 03 04 05 06 B C D E F G H A 01 02 03 04 05 06 B C D E F G H 363831291718 433929301919 433836311718 443929261717 443525271717 444025251516 483829271716 453425261617 B1: 24390B1: 43% Cell distribution is highlighted by software overlay Cell confluence & cell number estimation for each well displayed Repeated over several days Well selection 3% Day 15% Day 215% Day 355% Day 7 Growth curves calculated and displayed Electronically track and store plate data: cell confluence, cell number estimation, and growth curve View every growth curve in every well Assess cell growth objectively and quantitatively Rapid determination of the growth of cell lines is important for a number of processes, such as optimization of cell culture conditions and verification of monoclonality. However, conventional techniques are time-consuming, subjective, and may risk interference with cell growth: Tracking cell growth in 96-well plates is challenging and labor-intensive. Produce consistent results – in less time Save time and produce objective, quantitative, and consistent results by using the CloneSelect Imager to overcome the challenges associated with conventional techniques. • Growth rates accurately determined in every well of a single-well or multi-well plate • Cell confluence and cell number estimation displayed for each well • Growth curves calculated and displayed Fluorescence applications with the CloneSelect Imager FL Perform comparative confluence assays (red vs green) to identify and verify CRISPR edits. NEW! Assess cell viability Replace cumbersome colorimetric MTT assays with a non-invasive technique that enables monitoring over time.\* • Compare growth rates and colony size increase/decrease well to well • Track changes in cell density in response to various cellular manipulations or treatments to calculate dose response curves and IC 50 • Screen one microplate within three minutes • Label-free detection, no staining required. Avoid costly colorimetric kits \\* Accurate non-invasive image-based cytotoxicity assays for cultured cells, Marques-Gallego et al., BMC Biotechnology 2010, 10:43 Identify multiple nucleation points versus “edge only” growthIdentify sub-optimal environmental conditions or “edge-effects” Optimize cell culture conditions CloneSelect Imager has been used to rapidly screen culture variables to identify optimal culture conditions for low density or clonal outgrowth. Base-case – Day 7 data Additional information gained on cellular morphology and understanding of growth characteristics. 3Report Make confident, image-driven decisions throughout plate history Verify monoclonality After initial seeding, CloneSelect Imager can image every well, at any time point, using a ‘loci of growth’ functionality to highlight those wells that contain a single colony. • Focus on wells with a single loci of growth and view image history to verify monoclonality with the CloneSelect Imager using white light •Automatically verify monoclonality from day zero with fluorescense on the CloneSelect Imager FL Day 0Day 3Day 9Day 11 Single loci Yes! View image history of colony development Is colony monoclonal? White light Fluorescense Demonstrated IND success With a few simple clicks, the Monoclonality Report feature on the CloneSelect Imager objectively organizes the supporting image evidence needed to establish clonality into an easily shareable report, saving researchers hours typically required to do the same process manually. The Monoclonality Report is an audit-ready document that supports filing for an Investigational New Drug (IND) Application with the FDA (21 CFR Part 312). Example pages from the Monoclonality Report • Easily identify and select single-cell and artifact regions to include in report • Export high-resolution images of single cells, artifacts, and entire well (optional) • Automatically identify single cells with the CloneSelect Imager FL on day zero • Publish Monoclonality Report in PDF or Word format Wells captured on Day 10. The well on the left shows one colony while the well on the right shows two colonies. View entire well at final time point Quickly determine clonality of a cell line by visually inspecting the presence of multiple colonies in a single well. The reporting feature automatically generates the following data in a presentable format MonoclonalNot Monoclonal Monitor cell line development over time To characterize the growth from a single cell to a colony, cell regions can be designated and adjusted for each time point in a series. Monoclonal Day 0Day 1Day 2 A single cell on Day 0 and two cells on Day 1 confirm monoclonality. Not Monoclonal Day 0Day 1Day 2 Two cells are visible on Day 0, confirming that the cell line is not monoclonal. Not Monoclonal Day 0Day 1Day 2 Four cells have developed on Day 1, confirming that the cell line is not monoclonal. All Region ExportDay 0Day 1Day 2 Divide an entire well into individual images Export an entire well into 81 separate images to objectively confirm the absence of another cell. An image of a well is divided into regions, and a selected region is displayed over time. Highlight regions to review non-cell objects Selectively highlight parts of a well to differentiate cells from ambiguous objects. Day 1Day 2 Artifact Region ExportDay 0 A selected artifact region and its corresponding images over time is shown here. “Maximize success rate for serum-free colony outgrowth in chemically-defined media by prior optimization of growth conditions.” —Ben Hughes, Senior Bioprocess Engineer, NCRIS Biologics Facility, Australian Institute for Bioengineering & Nanotechnology (AIBN), University of Queensland Accelerate cell line development with a range of Molecular Devices platforms ClonePix 2 Mammlian Colony Picker Automatically screen more clones in less time than conventional techniques, select cells with optimal expression levels, and pick colonies with accuracy with the ClonePix™ 2 System. ClonePix systems are now used in over 100 laboratories around the world to increase workflow productivity, leaving more time to better characterize target proteins and run new projects. QPix 400 Series Microbial Colony Picker The QPix™ 400 series of microbial colony pickers offer you the unique option to simultaneously detect colonies and quantify fluorescent markers in a prescreening step before picking. QPix systems are used worldwide in over 600 installations in research institutes, biotech, and pharmaceutical companies. QPix robotics developed a famous reputation for reliability and accuracy in sequencing centers during the Human Genome project. SpectraMax i3x Multi-Mode Microplate Reader The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. CloneSelect Imager integrated with robotics from Beckman Coulter Photo courtesy of Beckman Coulter Corp., shows first generation CloneSelect Imager Process up to 75 lidded plates in a single run automate-it scara robot is recommended and supplied through Molecular Devices – optimized for CloneSelect Imager Automate with robotic solutions Electronic data tracking ensures control of high-throughput processes Unrivalled solutions based on exceptional imaging and intelligent image analysis CloneSelect Imager specifications ImagingCloneSelect ImagerCloneSelect Imager FL Software Software dedicated imaging software preinstalled on a high specification PC, Microsoft Windows 10 Software dedicated imaging software preinstalled on a high specification PC, Microsoft Windows 10 Light source White light imaging, trans-illumination Source: Xenon flash lamp (5 Watts) White light imaging, trans-illumination Source: Xenon flash lamp (5 Watts) Fluorescence: No fluorescence Multichannel fluorescence (GFP/RFP) Source: AURAIII light engine (LED) CameraHigh-resolution CMOS cameraHigh-resolution CMOS camera Imaging speed96-well microplate: 90 sec White light: 96-well/ 384 well microplate: <2 min Fluorescence: 96-well/ 384 well microplate: <8 min Objective4X4X ResolutionStandard: 3.6 micron High resolution: 1.8 micron Standard: 3.6 micron Instrumentation Source plate type Range of 6, 12, 24, 96 & 384-well SBS microplates Range of 96, 384-well SBS microplates Source plate capacity1 x plates1 x plates Instrument dimensions (height x width x depth) 45.4 cm (17.9 in) X 57.4 cm (22.6 in) x 72.4 cm (28.5 in) 45.4 cm (17.9 in) x 57.4 cm (22.6 in) x 72.4 cm (28.5 in) External light source: 16.3 cm (6.4 in) x 12.5 cm (4.9 in) x 26.3 cm (10.8 in) Instrument weight45 kg ( 99 lbs) 45 k g (99 lbs) External light source is an additional 3.6 kg (8 lbs) Regulatory approval ComplianceCECE QualityISO9001:2008 certifiedISO9001:2008 certified Automation compatibility API suite available for robotic integration. Please contact us for details. Our products offer scientists unrivaled solutions that utilize imaging and intelligent image analysis to support basic research, pharmaceutical, and biotherapeutic development. The systems continue to establish industry standards in areas such as picking microbial colonies for genomic studies or screening, and selection of mammalian cell lines. Other systems use imaging platforms to monitor cell growth, evaluate cellular responses, and quantify protein production. Through our expertise in robotics, cell and molecular biology, image analysis and interpretation, and supported by a strong IP portfolio, we are committed to the continual development of innovative solutions for life science applications. For more information, visit www.moleculardevices.com. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ©2022 Molecular Devices, LLC 1/22 1941F Printed in USA Phone: +1.800.635.5577 Web: www.moleculardevices.com Email: info@moldev.com Check our website for a current listing of worldwide distributors. \\* Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Italy, Netherlands, Portugal, Spain, Sweden and Switzerland Regional OfficesContact Us USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China +86.4008203586 Taiwan/Hong Kong +886.2.2656.7585 Japan +81.3.6362.9109 South Korea +82.2.3471.9531 India +91.73.8661.1198 ## Biopharma Reagents ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c1d41ae20531865dcb1c0deb448f15083d8d59c5590a6d8c28c1cfad6ce5d927.jpg) # Reagents and Supplies Get high-value clones faster and advance your BioPharma research with complete reagents and supplies ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/60f1cd456e0fc20c835c69f10f6001a65bcc870e2050e7884eb3c15b52bc2143.jpg) # Introduction # Unlock excellence in biopharmaceutical research. Welcome to the forefront of biopharmaceutical research and development with Molecular Devices. This guide showcases an exceptional range of reagents meticulously designed to streamline and enhance your biopharmaceutical workflow. In the dynamic realm of biopharmaceutical production, the right clone selection can significantly elevate results. With instruments, reagents, and supplies for microbial and mammalian cell users, you can retain the integrity of growing cell colonies, pave the way for automated identification and extraction of clonal cell lines, and accelerate the journey from concept to market. Embark on a path of excellence and innovation, where every product is a step towards reshaping the future of biopharmaceuticals. # Table of contents # 01 Cell biology reagents. CloneMedia CHO Growth A. 3 Recombinant CloneDetect. 3 CloneDetect.. 4 CloneMatrix and CloneXL. 4 Cleaning solutions... 5 Calibeads.. 5 02 QPix® Chroma Filters.. 5 QPix Chroma Filters. 5 03 Adjustable petri dish and microplate holders. 6 04 Bioassay trays.. 6 05 Microplates.. 7 Low profile microplates... 7 384-well high sample recovery plate.. 7 06 Deep well plates and lids. 8 Deep well plates. 8 Lids.. 8 07 Adhesive and heat seals. 9 Breathe seal. 9 08 Replicators.. 9 QReps polypropylene replicators. 9 09 Accessories.. 10 10 ClonePix Welcome Kits. 11 ClonePix Welcome Kit A. 11 ClonePix Welcome Kit B. 11 # 01 Cell biology reagents Cell culture media, supplements, and antibody-based reagents for use in cell line development of hybridomas, CHO and HEK cells # CloneMedia CHO Growth A The CloneMedia CHO Growth A semi-solid media portfolio provides a fast, simple, and comprehensive solution for CHO cell line development. Each batch of K8495 is subjected to rigorous safety assessment, with mycoplasma, virus and endotoxin full certification. Optimized for recombinant protein production. Free of animal components. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | CloneMedia CHO Growth A with L-Gln | 6 × 90 mL | K8800 | ClonePix | | CloneMedia CHO Growth A with L-Gln | 1× 90 mL | K8810 | | CloneMedia CHO Growth A without L-Gln | 6 × 90 mL | K8830 | | CloneMedia CHO Growth A without L-Gln | 1× 90 mL | K8840 | # Recombinant CloneDetect Newest detection agent free of animal origin for visualizing secreted antibodies. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | CloneDetect Human IgG (Fc) Specific, Recombinant, Fluorescein | 10,000U/1mL | K8495 | ClonePix, CloneSelect Imager FL | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6e7c2eed762c9760a7a0013af4a7bf39af3ecbfb36d9e84df750b7f16bfc3ba3.jpg) # 01 Cell biology reagents # CloneDetect Detection agents for visualizing secreted antibodies. All products are animal-derived, sterile, and azide-free. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | Human | | CloneDetect Human IgG (H+L) Specific, Fluorescein | 10,000U /1 mL | K8400 | ClonePix, CloneSelect | | CloneDetect Human lgG (H+L) Specific, Fluorescein, BSA Free | 10,000U /1 mL | K8202 | | CloneDetect Human IgG (Fc) Specific, Fluorescein | 10,000U /1 mL | K8205 | | Mouse | | CloneDetect Mouse IgG (H+L) Specific, Fluorescein | 10,000U/1mL | K8420 | ClonePix, CloneSelect Imager FL | | CloneDetect Mouse IgG (Fc) Specific, Fluorescein | 10,000U/1mL | K8425 | # CloneMatrix A $2 . 5 \\times$ concentrate that serves as a matrix to produce semi-solid media from $2 \\times$ concentrated liquid media provided by user Proven for hybridoma, myeloma, CHO, HEK and mESC. Supplied ready to dilute in $1 0 0 ~ \\mathrm { { m L } }$ bottles for convenience. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Partnumber | Compatibility | | CloneMatrix, makes 6 × 10O mL final media volume | 6 × 40 mL | K8500 | | | CloneMatrix, makes 100 mL final media volume | 1 × 40 mL | K8510 | | CloneMatrix-CHO: optimized for CHO cells,makes 6 × 100 mL final media volume; contains 6 vials of 2ml CloneXL and 6 botles of 40ml CloneMatrix | 6 × 40 mL | K8520 ClonePix | | CloneXL reagent: 5Ox concentrated animal component-free cell culture supplement converts CloneMatrix to CloneMatrix-CHO | 5 ×2 mL | K8525 | | | CloneMatrix-CHO: optimized for CHO cells,makes 100 mL final media volume; contains 1vial of 2ml CloneXL and 1bottle of 40ml CloneMatrix | 1 × 40 mL | K8530 | # 01 Cell biology reagents # Cleaning solutions Ideally suited to clean instruments and accessories. Removes proteins and biological materials from most metals, glass, plastic, rubber, fabric surface, and is safe to use with ultrasonic cleaners/washers. Environmentally friendly — contains no phosphates, chlorofluorocarbons (CFCs) or borates. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | aQuieandonentrtegdpinleaningsolution | 1L | K2505 | QPix400 Series | # Calibeads A must-have tool to calibrate, optimize, and keep your ClonePix instrument with optimal performance. This ready-to-use product is made from the mixture of fluorescent microspheres with 4 different wavelengths in a semi-solid substrate format. Microspheres fluoresce at four distinct wavelengths corresponding to the CFP, FITC / GFP, Rhodamine / PE and $C y 5 ^ { \\prime \\prime \\prime }$ filter sets of ClonePix systems. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | Calibeads: Fluorescent calibration beads for ClonePix. 2Oo μm diameter. Pan wavelength.Provided ready-to-plate (in 100 mL of semi-solid substrate, ca. 15-25 beads / mL) | 100 mL | K8150 | ClonePix | # 02 QPix Chroma Filter # QPix Chroma Filters ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/aec1e4ae86af3e25d9537def3a5a6e9466a4b0f0b14306c18b7190113d6f3cd0.jpg) Thin film optical filters for selection of bacterial colonies with multiple ranges of color intensities. Interchangeable filters last up to 10 uses and improve recognition of colored colonies for accurate picking of desired clones. Compatible with QPix Software Version 2.0 and higher. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Partnumber | Compatibility | | QPix Chroma Filter; Thin film optical filter for Blue/White colony selection | 1 filter | X1101 | QPix 400 Series | | QPix Chroma Filter; Thin film optical filters for Blue/White colony selection | 25 filters | X1102 | | QPix Chroma Colorimetric Colony Selection Software Kit; Blue/White Colony Selection Software Kit for QPix 400 Series. Includes software license and 2 QPix? Chroma Filters (X1101) | 1 license, 25 filters | X1103 | | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/656e8bb26ffa105d01888be17d883d121ad00898c96bc81e78d06eea9e8f0f66.jpg) # 03 Petri dishes and plate holders # Adjustable petri dish and microplate holders Spring-loaded holders automatically adjust to the perimeter of the plates to ensure secure fit of the plastic ware with a greater size variations. Black finish enhances optical imaging and reduces light scattering. Compatible with QPix Software Version 2.0 and higher. | | | | | | --- | --- | --- | --- | | Product name and description | Plate diameter | Part number | Compatibility | | 1-Way Adjustable Petri Dish Holder | 138-141 mm | X9401 | QPix 400 Series | | 2-Way Adjustable Petri Dish Holder | Any ANSI/SLAS 1-2004 microplates | X9402 | | 4-Way Adjustable Petri Dish Holder | 97-100.5 mm | X9403 | | 5-Way Adjustable Petri Dish Holder | 87- 90.5 mm | X9404 | # 04 Bioassay trays # QTrays Ideal for large scale culture and screening of bacterial colonies, regional picking and high throughput protein expression. The vented lid aids removal of condensation. Can be supplied with a 48-well divider to provide a cost effective alternative to petri dishes. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | Vented QTray with cover, polystyrene,240 × 240 × 20 mm, sterile, individually wrapped | 20 per case | X6023 | QPix 400 Series | | Vented Qtray with cover & 48-Well Divider, polystyrene, 242 × 240 × 20 mm,sterile, individually wrapped | 20 per case | X6029 | | # 05 Microplates A comprehensive range of 96- and 384-well microplates in a variety of profiles and materials. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/31ee2bd1402af02680a0d723cc95113839abc5a4af9f3c9ffcd2f1d005d6fac2.jpg) # Low profile microplates Low profile design maximizes robotic plate stacker volume and storage. Dimensions: $1 2 8 \\times 8 6 \\times 1 0 \\mathrm { m m }$ . | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | 384-Well Low Profile Microplate, round well, flat bottom, sterile, with cover, working volume 5O μL, packaged in sleeves of 10 | 160 per case | X6001 | QPix 400 Series | | 384-Well Low Profile Microplate, round well, V-bottom, sterile, with cover. Individually wrapped | 160 per case | X6004 | | 96-Well Low Profile Microplate, polystyrene, round well,flat bottom, sterile, with cover, working volume 18O μL, packaged in sleeves of 10 | 160 per case | X6011 | | 384-Well Low Profile, Large Volume Microplate, round well, flat bottom, sterile,with cover, working volume 6O μL, packaged in sleeves of 10 | 160 per case | X7001 | # 384-well high sample recovery plate Microplates for applications that require high sample recovery rates. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as $5 \\mu \ L$ . The low profile increases robotic stack capacity and storage. Dimensions: $1 2 8 \\times 8 6 \\times 1 0 \\mathrm { m m }$ . Working volume: $5 \\mathrm { - } 6 5 ~ \\mu \\mathrm { L }$ . | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | 384-Well High Sample Recovery Plate,polypropylene,without cover, working volume 5-65 μL,packaged in sleeves of 10 | 200 per case | X7020 | QPix 400 Series | | 384-Well High Sample Recovery Plate Plate, polypropylene, with cover, working volume 5-65 μL, packaged in sleeves of 10 | 200 per case | X7022 | # 06 Deep well plates and lids # Deep well plates Polypropylene deep well plates with elevated well walls for easier sealing. Conform to SBS standards. Non-pyrogenic, free of detectable DNase, RNase, human DNA. Ideal for storing sample materials, cell stock or cultivating bacterial, phage, yeast, suspension mammalian cell culture etc. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | 96 Deep Square Well Plate, round bottom, working volume 2O0O μL sterile, packed in sleeves of 6 | 24 plates per case | S1205 | QPix 400 Series | | 384 Deep Square Well Plate,round bottom, working volume 400 μL, packed in sleeves of 6 | 24 plates per case | S1207 | | 24 Deep Square Well Plate, conical bottom, working volume 10 mL sterile, individually wrapped | 25 plates per case | X7060 | # Lids QLid is a rigid polystyrene, close fitting lid. It is automation compatible, prevents cross-contamination among samples and educes excessive evaporation. | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | QLid, sterile, packed in sleeves of 10: fits S1205, S1207 and X7020 | 240 lids per case | X5101 | QPix 400 Series | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/8e135f9715dd2ff873142af718cacd9f18663a2749244a35e7adb531911b3fee.jpg) # 07 Adhesive and heat seals # Breathe Seal Porous film for sealing culture plates where air and gas exchange is necessary. Fits any ANSI/SLAS 1-2004 microplates. The $1 1 4 \\mu \\mathrm { m }$ thick film is easily pierced by pipette tips. Recommended for temperatures between -20 to $+ 8 0 ^ { \\circ } \\mathsf C$ . ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/120b95815aa92c60730d74bf7c7880a8c691d63da0dd25c7b18defdc890d1470.jpg) | | | | | --- | --- | --- | | Product nameand description | Quantity/pack size | Part number | | Breathe Seal for cell and tissue culture, non-sterile | 100 seals per pack | E1004 | | Breathe Seal for cell and tissue culture, sterile, packed in tamper proof bags of 25 | 50 seals per pack | E1005 | # 08 Replicators # Sterile QReps polypropylene replicators Increase processing speed by eliminating flaming/autoclaving, washing and using these pre-sterilized chemically resistant replicators. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d5c7690112716de2843b49ddb3ce6252cc20cf5c0eff75a38bb6f0a430da645f.jpg) | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | QRep 384 Pin Replicators: polypropylene,10 mm length (low profile plates), 1 mm diameter | 2 ×64 | X5050 | QPix 400 Series | | QRep 96 Pin Replicators: polypropylene,10 mm length (low profile plates), 1 mm diameter | 2 × 64 | X5051 | | QRep 96 Pin Replicators: polypropylene,10 mm length (low profile plates), 2 mm diameter | 2 × 64 | X5052 | | QRep 384 Pin Replicators: polypropylene,15 mm length (standard profile plates), 1mm diameter | 2 ×56 | X5053 | | QRep 96 Pin Replicators: polypropylene,15 mm length (standard profile plates),1mm diameter | 2 × 56 | X5054 | | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d4bc06d3e2b1e17b20cfc6062c564319ab8889f1b0feb85b1da4c18e2f31b661.jpg) # 09 Accessories | | | | | | --- | --- | --- | --- | | Product name and description | Quantity/pack size | Part number | Compatibility | | 130 μL ART pipette tips, filtered, sterile | 96 tips per pack (10 packs total 960 tips) | T0130 | QPix 400 Series | | DISPENKIT all-in-one pack for single-cell dispensing workflow, sterile | 4 sensing tips, 4 cell strainers,4 reservoirs, 5 vials of optimized medium,1box of 96-wide bore tips | 5306092 | DispenCell Single-Cell Dispenser | | DISPENSTAR pack for single-cell dispensing workflow, sterile | 4 sensing tips, 4 cell strainers,4 reservoirs, 5 vials of optimized media with beads,1box of 96-wide bore tips | 5307040 | # 10 ClonePix Welcome Kits # ClonePix Welcome Kit A (for CHO Users) Are you already a CHO Cell user? The CHO Growth A media portfolio provides a fast, simple, and comprehensive solution for CHO cell line development. Combined with the CloneDetect detection agent, CloneSelect® Imager, and ClonePix® 2 System, researchers can more efficiently develop new protein-producing CHO cell lines, thus accelerating time-to-market. • Optimized for high recombinant protein production • Ready-to-use culture media and supplements are free of animal components • Enhanced optical quality for colony imaging • One-step fast screening and selection of high-value clones # Kit (PN\# K9000) includes: ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/cb969b5bfd2b1709855c4acdc08aaa0dc9e5f04e36e728f89502871541fa7fd2.jpg) • 1 bottle of CHO growth A without L-Glutamine • 1 vial of Human CloneDetect • 1 vial of Mouse CloneDetect • 1 vial of CloneXL $5 0 \\times$ Concentrated Animal Component-Free Supplement • 1 bottle of ClonePix Calibeads # ClonePix Welcome Kit B (for non-CHO Users) CloneMatrix™ is an easy-to-use product that converts existing mammalian cell biology media to semi-solid form. Semi-solid media maintains the positional integrity of growing cell colonies, thus allowing the automated identification and picking of clonal cell lines. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/96c61dec082e353a3c5b72961a6dc52d3d3c182058f594fae10e291db9304fb3.jpg) • The use of semi-solid media combined with the unique fluorescent screening and picking technology of the ClonePix System will revolutionize clonal cell line development in biopharmaceuticals. • CloneMatrix is a unique methylcellulose concentrate that allows users to add their own media formulation to produce an optimized semisolid media reagent. • CloneMatrix is conveniently supplied as $4 0 ~ \\mathsf { m L }$ in a $1 0 0 ~ \\mathrm { { m L } }$ bottle, reducing the risk of contamination as there is no requirement to aliquot the concentrate. Supplied as a $2 . 5 \\times$ concentrate, simply add optimized media $2 \\times$ concentration) selection and supplements as required. • CloneXL is a $5 0 \\times$ -concentrated cell growth supplement proven to improve mammalian cell survival and support CHO, HEK293, stem cell clone screening, and single-cell cloning applications. It contains amino acids and thymidine family members with patented formulation. # Kit (PN\# K9001) includes: • 1 bottle of CloneMatrix • 1 vial of Human CloneDetect • 1 vial of Mouse CloneDetect • 1 vial of CloneXL $5 0 \\times$ Concentrated Animal Component-Free Supplement • 1 bottle of ClonePix Calibeads ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/de89df411037a474270f271f647f30fbdc4e17805dbcc55c83269711e253fc4f.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c7dba122e5c71da903cd55ab929d68d56609e7d89bb398b2ab019effba3cf819.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3de57b995b0072a34d1aac2bd2c2c167b2e40c6c88a81f16e16c3ce3e8a6b824.jpg) # ClonePix® systems Screening and selection of mammalian clones # QPix® systems Sets the industry standard in accurate colony picking # DispenCell™ Single-Cell Dispenser A simple single-cell dispenser for proof of monoclonality ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/bfc2c082416dff70b85a7234841d4d9819980976f5e482b2acb135c7f216e186.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/8829879c4503b805c6f1afff13661e4fa2fd8152a84bf42d9f894090b8fe2f49.jpg) # CloneSelect® Imager ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/4096f3879d23002c3960aac51af80e5e69dbe1c1a2b4bbd723de75896c468f81.jpg) \*Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom Objective, quantitative assessment of cell growth # CloneSelect® Imager FL Next-generation fluorescent imaging solution for the assurance of monoclonality # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## Custom Microbial Pickers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/dad57cc2e710f0650ebdef1d0ee87fe5e937255f49997691b92cb8133100f291.jpg) # QPix Custom Microbial Colony Pickers Tailored by Advanced Workflow Engineering Solutions (AWES) # KEY BENEFITS OF CUSTOMIZATION • A wide range of hardware and software customization possibilities to fit your unique workflow needs The AWES team provides comprehensive services from consultation through implementation • Automation features can be added in modules, so system capabilities can be upgraded through AWES at any time ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2aaa669d4d09511906636a6ddcda835a2f6a2c0e92d4e24aaf2e3fc6fe5bbf9b.jpg) # Get the highest level of flexibility in application and experimental design Our expert team of engineers and application scientists can tailor the QPix™ systems to meet your workflow demands for automated microbial colony picking and plating. # Reduce hands-on FTE time with robotic operation Add in robotic automation to increase throughput, eliminate human errors, and achieve consistent sample handling. • Modular automation design—components can be added in modules and are upgradable Keep samples & people safe and clean Minimize contamination with optional additions of HEPA filters, interlocked safety guards, and more. # Suit your unique picking needs with custom picking and plating Configure picking heads, plate holders, plating and streaking patterns, and software tools for those non-conventional microbes. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/73b2940caa29b5c8577f5dae91c99520a324354b2e26279118a5c063eda65e4f.jpg) Accommodate a wide range of labware Modify the QPix decks to be compatible with a variety of standard and non-standard labware. Screen colonies based on attributes such as size, color, and fluorescence Customize cameras, filters, and detection algorithms to see and pick more. • Modes include red/white and blue/white, fluorescence Be more efficient managing data Simplify workflow, audit trails, and file import and export with software customization. • I ntegrate with LIMS and data management systems through API # Examples of AWES projects # PROJECT 1 # ntegrating QPix system into a fully automated, high-throughput DNA manufacturing workflow The Edinburgh Genome Foundry (EGF) is dedicated to automated design and assembly of large DNA constructs using a fully automated robotic platform. They needed an automated colony picker that can be integrated into their existing DNA assembly workflow, which included 3 robot arms, 2 liquid handlers, incubators, and PCR machines. The AWES solution: • Modified the QPix system structural design to allow for robotic arm access • Developed a custom picking head for the spreading of 8 samples onto a single, undivided agar tray • Provided open API and software support for the integration process # Results • The modified Qpix system provided the necessary high throughput sample handling (3,000 colonies/hr) • I ntegrated, reliable sample tracking for effective sample data management • Decreased FTE labor and reduced operational costs ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d4535663e4e5a88325f14723e36e123ef3d0e24b90d3a8cc7308e717ddf28ab1.jpg) Modified QPix system. The back of the instrument has been customized to allow robotic arm access. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/2c1c04d65932cfd68c7773dae29805b8fd5189f454198ebbfcb9b953a3286803.jpg) Part of EGF’s fully automated setup. The modified QPix system (red arrow) is in the upper left, behind the robotic arm. A second robotic arm is also visible, approaching a plate hotel. # PROJECT 2 # Modifying QPix system for high-throughput screening in protein evolution studies In this project, Customer $\\mathsf { X }$ studies directed evolution of proteins. An essential step in their workflow is screening libraries of proteins and antibodies in bacteria, a task that involves that requires colony picking and inoculation. They required a system that can pick and plate over thirty 96-well standard and deepwell plates per day. The AWES solution: • Modifies the structural design to allow for robotic access • I ncludes an automation platform powered by Green Button Go software, Preciseflex PF3400 sample mover, ambient plate and QTray storage (88 plates), and clean air HEPA enclosure. CAD drawing of the QPix setup. (1) $\\mathsf { Q P i x }$ system modified to allow robot access. (2) Plate storage. (3) Preciseflex PF3400 sample mover. (4) HEPA enclosure. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/14990b03ec13f9ddb9aac6f09f1d5da1f8349fe33f64136ddb8e4936db9bfdf2.jpg) # Goals • The modified Qpix system is intended to provide the necessary high throughput sample handling (3,000 colonies/hr) while simultaneously processing over 80 plates per run • Decrease FTE labor, increase throughput, and reduce operational costs ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d786fea81f05e78780959525c53125ea115367ed48a38c1538b4ee9ae7d048f4.jpg) # Turnkey service Our AWES team works with you to match our service offerings with your needs. Our solutions are tested and validated in-house before being installed in your lab. # Scale-up automation We can deliver a customized, automation-ready QPix system for future integration; adapt it to integrate into an existing automated solution; or design and test an entire workstation from scratch. # Hardware customization From custom labware, to unique transfer pins, to special handling requirements, we’ll work to find a solution and prove it through validation. # Explore modifications with AWES The Molecular Devices Advanced Workflow Engineering Solution team has successfully tailored QPix colony pickers for some customers to include customized software and hardware as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell.\* \*Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance. # Software customization We can match specific API needs, enable unique workflow capability, integrate into LIMS or data management systems, customize a UI, and/or control any custom designed hardware. # Biological validation Our application scientists work with you to understand your process and biological requirements, to reproduce and test the actual performance in-house prior to shipment. # Global service and support With over 30 years of experience in the life science industry, you can count on Molecular Devices to deliver quality products and worldwide support. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/3e3d589e2718272580d4dc74b2a97325d34f9bafa083ca2774b5cfbf816d65f7.jpg) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada China (Beijing) Japan (Osaka) +1.800.635.5577 +86.10.6410.8669 +81.6.7174.8331 United Kingdom China (Shanghai) Japan (Tokyo) +44.118.944.8000 +86.21.3372.1088 +81.3.6362.5260 Europe\* Hong Kong South Korea 00800.665.32860 +852.3971.3530 +82.2.3471.9531 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ©2018 Molecular Devices, LLC 7/18 2156B Printed in USA ## Molecular Cloning Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/ab5a408c46d4a7087304ee9bdbce6013aef431694666c0111845abfb4a8cf400.jpg) # Molecular Cloning Systems designed for efficiency, ease of use, and consistent results ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/e2d77553c1d620246d13a9bf57513f604c21f436fd1363f6472c269ba48f700a.jpg) # Introduction Our solutions for antibody discovery and cell line development provide dedicated, scalable, and easy-to-use products for establishing clonal populations. The systems feature a selection of options and models to address your specific research including multiple imaging modes, biology-specific pins, fluidics, and environmental control. These solutions combine intelligent imaging with analysis and automation to increase throughput, consistency, and provide image-based documentation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/238342e40debe0cb16f99bb42390c4be789e85e11573ae6eaadc179e6b7f88b4.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/4d0159193ecc521c77a20da56478d01290af433190bd6b1e5929d41ac1298c0f.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fcad1fe65886eabfcecec0a573b077104ea1cd47470f6072abbddfca12634927.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/17f81f8a4b05298f1fb6b75c6553cbe1f302d9e1d0a09c9261f5754bec77313c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d45ae36cae73fdf33db70c19e08fcdf9259246eda8332ba9697dfed2c0f92ab6.jpg) # Verify monoclonality easily Sort viable single cells efficiently Streamline workflows # Improved probability of finding rare clones of interest # QPix® 400 Series Microbial Colony Pickers # Featuring the all-new QPix XE The QPix XE is a compact microbial colony picker ideal for labs with reduced space, but need efficient colony picking through put. The QPix XE is a great entry level system to replace highly subjective and error prone manual picking with automated colony picking. # Automated microbial clone screening and library management system The QPix 400 Series Microbial Colony Pickers combine intelligent image analysis with precise automation for fast and efficient screening of large libraries. With a variety of data tracking and assay tools, the QPix Software streamlines the control and management of complex and iterative processes. • Use the QPix system for synthetic biology, DNA assembly, antibody discovery, protein engineering, and phage display workflows • Streamline your workflow with scalable-automation – pick up to 30,000 colonies per day • Electronic data tracking for well-documented data control ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/c59dce3ada9068e703d1349835dafc85dbd8ac77a76b3f75dc903dc03d761c00.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/09794f1eb2950df218df721a6da5a85148480b5cae3c2621235387e96d67354d.jpg) QPix 420 system ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/56e2ff3c3910b29a39b5a111b180573dfec282f91cba97cb738a7ef71d602ff6.jpg) QPix 450/460 systems ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/6bc27313d933f460cc4747c36e1cc591ede81fb1b3c9ba064bc4ed05681a3339.jpg) QPix HT system ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/83fa408ab62dae0acfbbbbfd15821c1961e39d98ba8920baa03c8e36af1587ac.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/fde7468aa1cfa4be9c5555d9be810f4d15abace5b2400eafb35a5ef54a8e9f0f.jpg) # SpectraMax® iD5 Multi-Mode Microplate Readers Microplate readers are commonly used to measure the quantity and quality of DNA during DNA isolation and purification protocols. By using a microplate reader to measure DNA concentration and purity, scientists can ensure that their DNA isolation and purification protocols are working correctly, and that they are obtaining high-quality DNA for downstream applications. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/d3bf97426fe38c51e6478cb6da37b820b20c0899d6d5a62fcc77ec59307c1009.jpg) # To use a microplate reader for DNA isolation validation, the following steps can be taken: 1. ``` First, the DNA samples are prepared and added to a UV transparent 96-well plate in appropriate dilutions or concentrations. ``` 2. ``` Next, the microplate reader is programmed to measure the absorbance of the DNA samples at specific wavelengths, usually at $2 6 0 \mathsf { n m }$ and 280 nm. The absorbance values at these wavelengths can be used to calculate the concentration and purity of the DNA. ``` 3. ``` The software in the microplate reader can then be used to generate a standard curve to convert the absorbance values to DNA concentration. ``` 4. ``` Once the standard curve is generated, the absorbance values of the DNA samples can be measured and the DNA concentration can be calculated using the standard curve. ``` 5. ``` The 260/280 absorbance ratio is also calculated to determine the purity of the DNA samples. A ratio of 1.8 is considered to be indicative of pure DNA. ``` 6. ``` Plate reader also can be used to run spectrum scan (230–320nm). This will show if there are any contaminants. ``` ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a77fbf4621380bb416c82743de42ca45eb830aede05fa98dab889550d54ccb4a.jpg) # DispenCell™ Single-Cell Dispenser # Automation for fast, easy and gentle single-cell isolation DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop. • Confidently isolate single cells and provide consistent, high-quality evidence of monoclonality to the FDA • Highly efficient single-cell sorting with an average monoclonality reliability of $> 9 0 %$ observed • Extremely gentle handling of the cell sample (less than 0.1 si), while preserving cell viability and outgrowth • Minimize cross contamination through disposable cell dispensing cartridges • Fast turnaround time—prints a 96-well plate in less than 10 minutes (384-well plate in less than 40 minutes) • Sort wide range of cell lines: CHO, HEK, SF9, iPSC, primary cells • Compact footprint takes up a small amount of valuable bench space # Verify monoclonality confidently at day zero with a CloneSelect Imager FL and DispenCell bundle # Streamline your workflow when you pair the DispenCell with the CloneSelect Imager FL The all-new CloneSelect Imager FL adds high contrast multichannel fluorescent technology in addition to the standard white light imaging that allows for accurate single-cell detection and proof of monoclonality at day 0. Identify and verify gene edits with comparative confluence assays. • Document evidence of single cells and confluency digitally for auditing and submission to regulatory authorities • I mage cells non invasively at multiple time points to monitor colony formation • Screen using high resolution white light imaging • Deliver real-time results with on-the-fly analysis • Automation and integration ready ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/bpd/images/a5e21e96be8ae309fcf783c4cfcb2f6ff09380357541b780b68160716bf31ed5.jpg) # Get started. Explore single-cell dispensing with us. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 ## SpectraMax i3 Platform ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a9b826bb4cb3ac207aa7773014205d095653530029457b3fb4fbf6eacf141847.jpg) # Key Features • User-upgradeable application cartridges and imaging cytometer option • Sensitivity across spectrum with Spectral Fusion Illumination • Expanded dynamic range • Control and analytics provided by SoftMax Pro Software # SpectraMax i3 Multi-Mode Platform # A flexible, user-upgradeable microplate detection system The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. # Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. # Superior optics Monochromator optics support Absorbance, Fluorescence, and Luminescence, while user-exchangeable cartridges expand the system’s detection to Time Resolved Fluorescence, HTRF, Fluorescence Polarization, AlphaScreen, and Western Blot detection modes. Combined with the SpectraMax MiniMax Imaging Cytometer option, this platform is the most versatile microplate detection system available on the market. The SpectraMax i3 System not only offers unlimited flexibility with our patented cartridge architecture, it also uses advanced Spectral Fusion™ Illumination as the excitation source and provides extended dynamic range with a patent pending design. Spectral Fusion Illumination is a combination of a flash lamp and powerful LEDs, producing a powerful light source, ultimately increasing the sensitivity of the system across the full spectrum. The dynamic range extension uses a combination of optical and electronic components to not only provide optimal sensitivity, but also maximize the signal range. # Superior software Supported by industry-recognized SoftMax® Pro Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. # Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate • SpectraTest® Validation Packages (ABS1, FL1, LM1) • SoftMax Pro ${ \\mathsf { G } } { \\mathsf { { x } } } { \\mathsf { P } }$ Microplate Data Compliance Software • IQ/OQ Protocols Adding modes and functionality is minutes away. Insert a cartridge to perform new applications. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/507c5f97affaf79de3c4cfda6eaf309ee348a2b006269ef6f1fb2530d11b6c88.jpg) New applications in minutes ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c1b4e4aba1d8287d4b03dca9b04ed1157b29979a7552e5ebe30e9254a7b12524.jpg) Spectral Fusion Illumination A powerful combination of Xenon flash lamp and light emitting diodes (LEDs) provides unmatched signal strength and superior sensitivity across the spectrum. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/53729dd04696b28b5f7872312c4a5403ad7c8aa3e101dd0302f457e875b687fc.jpg) Live cell imaging Top: The SpectraMax MiniMax Imaging Cytometer option allows for live cell images and analysis. Bottom: The ScanLater Western Blot Detection Cartridge enables protein detection. | | | | | | | --- | --- | --- | --- | --- | | General specifications | Absorbance photometric performance | | Dimensions (in.) | 12.63 (H) × 15.38 (W) × 23.38 (D) | Wavelength range | | Dimensions (cm) | 42.23 (H) × 39.05 (W) × 59.37 (D) | Wavelength bandwidth | 230-1000 nm 4.0 nm | | Weight | 68.3 Ibs. (31.0 kg) | Wavelength accuracy | ±2.0 nm | | Power consumption | < 200 watts | Wavelength repeatability | ± 1.0 nm | | Power source | 100-240 VAc, 2 A, 50/60 Hz | Photometric range | 0-4.0 OD | | Robotic-compatible | Yes | Photometric resolution | 0.001 0D | | General photometric performance | Photometric accuracy | < ±0.010 0D ±1.0%, 0-2 0D | | Plate formats | 6 to 1536 wellss | Photometric precision | < ±0.003 0D ±1.0%,0-2 0D | | | Spectral Fusion llumination | Stray light | | < 0.05% @ 230 nm | | Light source | (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges) | Fluorescence intensity performance | | Reading capabilities | Microplates, cuvettes (via adapter) | Wavelength range 250-850 nm | | Detectors | PMT and/or photodiode | Wavelength selection | 1.0 nm increments Adjustable | | Shaking | Linear and orbital | Bandwidth (EX/EM) | EX 9/15 nm EM15/25 nm | | Injectors | Option available | Dynamic range | \> 6 logs | | Temp.control Temp. uniformity | 4°C above ambient to 45C | Top sensitivity (fluorescein) | Optimized Guaranteed\*\* | | Temp. accuracy | ± 0.75°℃ | 96 wells | 0.5 pM | | Environmental control | ±1C at 37C set point | 384 wells | 1 pM | 3 pM 4 pM | | Spectral scanning | Gas quick connect Abs, FI, Lum | Bottom sensitivity | | | | Endpoint reading | All modes | (fluorescein) | Optimized | Guaranteed\*\* | | Kinetic reading | All modes | 96 wells | 5pM | 10pM 20 pM | | Well scanning | Over 20 by 20 in all modes | 384 wells | 5pM | | Wavelength selection | 1.0 nm increments | | Luminescence performance | | Standard read times (minutes:seconds\]\* | Wavelength range | 300-850 nm | | | 384 wells | Wavelength selection | waveci | | Absorbance | 96 wells 0:30 | 1:40 | | | Fluorescence intensity | 0:25 | Dynamic range | \> 6 decades | | Luminescence | 1:25 1:15 | Cross-talk Sensitivity (ATP-Glow) | < 0.3% in white 96- and 384-well microplates Optimized | Guaranteed\*\* | \\*\\* For properly functioning, operating, and maintained equipment Additional cartridges | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | | | | Cartridge | Description | Name | Specifications | Sptimivity | Gunratited | Slots | | ScanLater | Western blot detection using ScanLater Western Blot Assay Kit TRF-based with 340/80 nm EX and 616/10 nm EM | 0200-7027 | EX range: 340/80 nm EM range: 616/10 nm | High fg levels of Streptavidin | High fg levels of Streptavidin | 2 | | AlphaScreen | E (100) EM filter Pickia Guaranteed sensitivity: < 100 amol phosphorylates biotin-peptide in 25 μL assay volume in a 384-well plate | 0200-7017P0S | 96anda4Wel pates | | < 100 amol (384-well) | | | 0200-7018P0S | 96andhaelplates | | < 100 amol (384-well) | 1 | | 0200-7019P0S | Alpha 1536 HTS 96, 384,and 1536-well plates | | < 100 amol (384-well) | 1 | | HTRF htrf | Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters Measures both emissions simultaneously | 0200-7011P0S | 6- to 1536-well plates | Exceeds Cisbio certification requirements | 2 | | TRF TRF | LED light source and Europium EX and EM filters (370-616 nm) Suitabheii Includes 642 nm EM filter for TR-FRET assays with Samarium labels | 0200-7008P0S | 6- to 1536-well plates | 96-0-0.01.M 1536-0.08 pM | 394-well:065PM 1536-well: 0.19 pM | | | FP | Fluorescence Polarization detection for 6- to 1536-well plates | 0200-7010POS | Fluorescein FP EX 485 nm, EM 535Pan Ss EX 535 nm, EM 595P and 595S nm | | | 1 | | LUM LUMI | Glow luminescence detection for different plates | 0200-7014P0S | Glow for 96-well plates | 96-well: 1 pM | 96-well: 1.5 pM | 1 | | 0200-7015P0S | Glow-wel p-ad | 96-wl:12 0M | 36-wel:24PM | 1 | | 0200-7012P0S | Glow for 96-,384-,and 1536-well plates | 96-wl:1.5 0M 1536-well: 19 pM | 96-wel:2.0m 1536-well: 25 pM | 1 | | LUM Dual-Color LUMI | Dual-color luminescence detection for 6- to 384-well plates | 0200-7016P0S | 410 and 5152mi EM ites | No certification specifications available | 1 | | 0200-7013P0S | Chroma-Glo luminescense assay with No certification specifications available 510 and 610 nm EM filters | 1 | | FI | Fluorescence cartridges with specific LED EX light source and EM filters | 0200-7002P0S | ExCoumminM465/535nmm | | 154-wel1-130 | 1 | | 0200-7003P0S | Fluorescein-Rhodamine EX 485 nm, EM 535/595 nm | | 384-well: 1.3 pM 1536-well: 3.75 pM | 1 | | 0200-7004P0S | EX 535nmy3C5/655m | | 1534-wel12.0 | 1 | | 0200-7005P0S | CFP-YFP labels EX 445 nm, EM 485/535 nm | 384-well: 67 pM 1536-well: 625 pM | 1 | | # Visualize cells with your microplate reader ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a243a610828d2d437780cfb21121b3f2a343716ad03e2d668182e5e9c8bdea93.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/90061e0feddb8584991726d12daefb4ae96e1fbce5fdb41370d37e4d386d66b1.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/97d3063fc626859c237fa43d29f6b483eb1421d662d613d2695c03d99eddf4f4.jpg) Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. The plate is set up for reading and images are acquired according to specified parameters. Cells in each image are identified by SoftMax Pro Software and cellby-cell statistics are collected. Data are then analyzed and visualized in different graphical representations. The SpectraMax MiniMax Imaging Cytometer is controlled by the wellestablished SoftMax® Pro Software, and is further enhanced by the analysis capabilities of the MetaMorph® Software backbone. Powerful cell identification and image analysis are supported in fluorescence mode and easily accessible within the software. In the settings interface, users can select between fluorescence or transmitted light, define plate type, read area and number of images per well, plus specify positive/negative wells # Contact Us # Regional Offices Users can also select fluorescent analysis types with corresponding output parameters as follows: | | | | --- | --- | | Analysis types Output parameters | | Cell count | • Cell count ·Average area ·Average intensity · Average integrated intensity | | Cell proliferation | • Covered area | | Marker expression | ·Expression in image | | | | | | | | | --- | --- | --- | --- | --- | --- | | Phone: | +1-800-635-5577 | USA and Canada | +1-800-635-5577 | Japan (Osaka) | +81-6-7174-8831 | | Web: | www.moleculardevices.com | Brazil | +55-11-3616-6607 | Japan (Tokyo) | +81-3-6362-5260 | | Email: | info @ moldev.com | China (Beijing) | +86-10-6410-8669 | South Korea | +82-2-3471-9531 | | Check our website for a current listing of worldwide distributors. | | China (Shanghai) | +86-21-3372-1088 | United Kingdom | +44-118-944-8000 | | | Germany | 00800-665-32860 | | | FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2013 Molecular Devices, LLC • 10/13 $\\cdot$ Printed in USA • PN: 0120-1567.D ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7e40a79003d06ab857088910b92f2eb5d716e93d110b9202611e4ceb85cb174e.jpg) Unstained CHO cells observed under transmitted light using the SpectraMax MiniMax Imaging Cytometer. This upgradable option allows quick visual inspection of cell health prior to plate reading without switching instruments. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f4f043ddf409f61b93d5b30b20ca2d3fcc0bdedd37d0b86452e1a55edc59eab6.jpg) # Molecular Devices ## Automated Drug Discovery Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/0f57781eecd195eb4270852e3101a9a06b9f683cf272bfae09b0636f88cd4707.jpg) # Innovative Solutions for Drug Discovery and Life Sciences Research Advancing Discovery ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5ede30d5484635f44352d6a8dcee426830f65ee562c5aed71c73712e5ba7e186.jpg) # The future of cell culture backed by data-driven science. CellXpress.ai®Automated Cell Culture System automates 3D biology, improves workflows, and makes assays more reliable and reproducible. # CellXpress.ai Automated Cell Culture System AI-driven cell culture innovation hub that automates processes, improves workflow, and makes assays more reliable and reproducible with machine learningassisted monitoring, feeding, imaging, and scheduling This revolutionary system gives your team total control over demanding feeding and passaging schedules— eliminating time in the lab while maintaining a 24/7 schedule for growing and scaling multiple stem cell lines, spheroids, or organoids. The CellXpress.ai cell culture system can give your lab the confidence in experimental outcomes to make key decisions sooner, achieve milestones faster, and get to clinic earlier with lower attrition rates. All of it backed with the assurance of a full event log to confirm on-time feedings and critical task execution with complete digital microscopy records. # Key features ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/54f8bba1ca9f2925ba3445d5567609a5f75d33fa428c69037b182c08160059d1.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c54e2324fea6b6edb867179c0c1371661861cb9ee481972f9675d318495e1930.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a231efd7ed7498d456002946fc8a6f5c2438b97c3388af2049dc41f7e9ada134.jpg) # Automate your cell culture processes. Total control over demanding feeding and passaging schedules reduces hands-on time in the lab. # Improve your screening workflows. 24/7 operation maximizes productivity for growing and scaling multiple stem cell lines, spheroids, or organoids. # Develop reliable, reproducible assays. A machine learning-assisted solution standardizes the development process to deliver consistent, unbiased, and biologically relevant results at scale. # Key capabilities # Scale up complex cell culture workflows. Actionable imaging and turnkey protocols for reliable media exchange, monitoring, and passaging. Automated cell culture and image analysis workflows run 24/7 – even when your lab is closed. # Evaluate & make decisions earlier. Answer critical questions sooner easily identifying outliers at the well, plate, or experiment level to help detect variability sources. Save reagents by removing these plates or wells from downstream processing early in the drug discovery process. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7d36f5b798e7916dd48798b40de5bab3804826c44144e9d8a962ebc065a3c533.jpg) # Reduce human error. I mprove productivity and optimize hands-on time with image-based, deep-learning decision-making. Remove variability, maintain sterility, and increase confidence in success with automated cell handling. # • Standardized protocols. Reliable and consistent automation speeds the development process. Real-time feedback alerts users to milestones or events and generates automated tasks to resolve them. # Track the complete cell journey over time. • Turn data into decisions. A unified software environment makes it easy to develop traceable and reproducible cell cultures specific to your desired assay endpoint. Solve complex image analysis problems utilizing advanced artificial intelligence (AI) to transform images into results. User-friendly workflows help you get answers faster from 2D, 3D, and time lapse experiments. Designed for continuous operation, the CellXpress.ai Automated Cell Culture System boasts a software environment that will keep you from worrying about missing a feeding or passaging step. Offering a wide range of features to make your cell culture process more efficient, reliable, and reproducible, the CellXpress.ai system brings together single-source initialization, protocol failure protection, and machine learning-enabled decisionmaking to streamline the cell culture process and makes it easier than ever to navigate and monitor even the most complex workflow. # EXPERIMENTAL RESULTS PANEL Flexible ‘widget space’ allows the user to construct their own data view appropriate for their experiment. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/07c84a4427841cec297256dbd664da7f5f868b949ba14586f99ef571bbd46e6e.jpg) # PASSAGING PROTOCOL Easily create protocols with an intuitive interface designed for biologists. Add building blocks for each phase of the workflow, including seeding, feeding, incubation, harvesting, passaging, imaging, and analysis. # CELL JOURNEY Track your cell culture experiments over time and across passage events. # Multi-mode microplate reader portfolio # Key features Improved sensitivity across entire spectrum Super cooled PMT for improved luminescence range ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c0966bc60b2876fd5cda97e6abf325cb4c0bb4f8f063af29e04cfe2a3ca9d958.jpg) Flash assays captured easily using SmartInject™ Technology Library of user-configurable applications to choose from ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/72af4535c90e99a09e3cee39195aaafca0fdfd40f6eb97d6533547cbe69e7f26.jpg) Pipette errors eliminated for true OD measurements with no temperature dependency Automation-compatible options ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/247860de68cc982a889635fdcd4886a77dbba3ae561a1bca7bfe3138c32477e5.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/0f5ecdb88429cdd83283af7f66b876522c315b61513b2e324b4472c03a03361e.jpg) # SpectraMax® iD3/iD5 Unlimited breadth of user upgradeable application modules Multi-user microplate readers with a large touchscreen and NFC functionality • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability imaging, and quantitation of nucleic acids and proteins on a single reader • Optional western blot detection • Built-in near-field communication (NFC) functionality for access to custom protocols and results with a single tap • Enhanced touchscreen workflows for walk-up usability • Ultra-cooled photomultiplier tube (PMT) detector for background noise reduction • QuickSync feature pushes data to any computer within the same network • Flexibility to set up experiments using either filters, monochromators, or a combination of both on the SpectraMax iD5 reader # Multi-mode microplate reader portfolio ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/ed492d18b40a1dc9d07bb0da257f39e237bbbe6dab98fa49c520f503406f0e15.jpg) # SpectraMax® Mini ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e95cb7eeca30212c95dbd714971db49e9b8764ce6e12790dd23d7372b80bb052.jpg) Budget-friendly multi-mode microplate reader with best-in-class data analysis software # SpectraMax® M Series Configurable readers with triple-mode cuvette ports, validation tools, and compliance software • Three modes of detection for UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubes • Xenon flash lamp for detection of a lower concentration of samples over a wide range of wavelengths • Best-in-class SoftMax® Pro software offering pre-configured protocols for most commonly used applications • Choose from varying reader modes (2–5) • Dual monochromator tunability • Automated absorbance pathlength correction • Endpoint, kinetic, spectral and well-scanning • Validation and compliance tools • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7a6c8860594ac0dc88f12ccc4d0eb2a5d7771fc7bf06d9ea96bb7362c64db234.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c8b428116b803cf3a006825cf0b631bffbfda6b46ed19a23cd9ed410706bcaa6.jpg) # FlexStation® 3 High-throughput screening capability on one fast, configurable microplate reader Precise optics, fluidic transfer, and assay flexibility on one integrated microplate reader • Automatic Z-height optimization for fast top- and bottom-reads • Manual gain adjustment eliminated with reads up to seven logs of sample concentration in a single pass of a plate • Multiple calculations performed on one data set • Side-by-side comparison of multiple experiments • Fastest read times for 96–1536 well plates • Robotics compatibility • Five-mode reader with a wide range of applications • User-definable liquid transfer enables multiple live cell kinetic assays • User-defined pipetting simplifies assay optimization • Instrument and software validation • Benchtop FLIPR® system • Robotics compatibility # Absorbance microplate readers # Key features ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/eff0f401640a135fceee3d9a3822e74b417f43b800cd0f487a4109d6a74c2747.jpg) Two-fold improvement in speed Small footprint Automated injector workflows ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/8ca98adc9a252c39857d5838885172be190db8452057256d2cbdca9de6769c7a.jpg) Pipette errors eliminated for true OD measurements with no temperature dependency Spectral resolution ensures accuracy of DNA absorbance measurements DNA, RNA, and protein quantitation Predefined protocols with SoftMax Pro Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4a98417cbea9f04d7eb1aec449db274048809070124262381e028e6a4037d917.jpg) # SpectraMax® ABS/ABS Plus Microplate Readers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/9b17f2364b3e5cf236b55198332148e6000279d93bfdd59d7a855d5972639ad0.jpg) # SpectraMax® QuickDrop Micro-Volume Spectrophotometer Fast absorbance detection for a wide range of assays without the use of filters One-drop for a full-spectrum micro-volume absorbance spectrophotometer • Compact design • 96- or 384-well microplate compatibility • Eight-channel optics for fast reads • Pipetting errors eliminated with patented PathCheck Sensor to measure the optical pathlength of samples in a microplate • Validation and compliance tools • Easy to use • Touchscreen interface with preconfigured analysis methods • Fixed path length requires no calibration • Micro-volume sample port has no moving parts to break or skew results regardless of viscosity • Data can be exported easily to a USB drive for additional analysis • One-swipe cleaning # Fluorescence microplate readers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/be0132131995545bc90e7ac888183ac5abc89d69ee284bc54bb086e2f38db932.jpg) Fluorescence detection without filters Gemini™ XPS/EM Microplate Readers • No filters needed • High level of sensitivity • Validation tools • Robotics compatible # Handlers and accessories ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/40cf5fefd6e2fcc06f0311fda283b6d8656d859636e38f0e280b872b24b95d09.jpg) Simple, economical microplate automation for any lab StakMax® Microplate Handling System • Integrates with SpectraMax readers • Stacks up to 50 plates • Robust and reliable • Easy to set up and use • Can recycle plates for multiple reads • Leverages the power of SoftMax Pro Software # Luminescence microplate reader ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e94328f5ff06536ea0a1ddb913bf54f17575b4a7f60c80cfd1ce073f14c9deae.jpg) SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384- well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4bdf228cfd93a2ba12ba59bd922f5b34ef149419ec99bdea53c370ec50367a79.jpg) Smaller, faster quantitation of DNA, RNA, and protein SpectraDrop Micro-Volume Microplate • Micro-volume detection for 1-64 samples • Elegant simplicity for multiuser labs • User versatility with different volume size and density options • Multi-channel pipettor can be used • Cleaning with hasslefree handling # SoftMax® Pro Software # SoftMax® Pro GxP Software The most published microplate reader control and data analysis software • Software powers discovery on all Molecular Devices readers • Simplified data measurement through library of prewritten protocols • Software validation protocols available for IQ/OQ/PQ of hardware and software • Import and analysis of complex data acquired from any scientific instrument • Over 160 built-in protocols with preconfigured assay parameters for a wide variety of common assays • Discontinuous kinetics feature allows for custom assay workflow and runs multitask kinetics • Complex, customizable curve fittings • Flexible data output formats support research and publications Meet FDA guidelines in GMP/GLP labs with complete validation tools ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/158c41bcb665db2ab2a7789a41913a1f26b5e1e8c26ca98f0649a3cc31953d96.jpg) • System audit trail for tracking and recording all actions and for paperless documentation with eSignatures • Microsoft SQL database for complete control over file access permissions • Document workflow and status system for maintaining data integrity with control over document workflows • Windows Active directory for authenticating and authorizing all users • Signing and approving different steps of a document • Assigning users to different projects with different roles • Auto export of signed XML files ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/52f21d226e5b6733190969c37b62fce122bf1487d3c1ffdb235bf4357476a8ec.jpg) # Validation plates # IQ/OQ/PM services The SpectraTest® Validation Plates provide automated, comprehensive, and traceable validation of optical performance, plus automatic verification of our microplate readers. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/640d2509e473754a555fea403b85ba1150c720c6a8bce7aac21f11e3d5151dbe.jpg) # Automated for ease of use All test measurements and calculations are handled automatically by the SoftMax Pro Software protocols. Should any of the measurement parameters fall outside defined limits, a test failure is reported with the suspect parameters identified. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/18679771ae1b8264afa578921dc331595c2aa774ee13338e36eaa6eca48f3d6c.jpg) # NIST traceability Calibration of the plate’s filter standards is accomplished through the use of an instrument calibrated with primary NIST standards. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/57226e72afdcdcaf0b1b70b771b99d180d8a4b84a41d95a3c4837e98fa9ab012.jpg) # Recertification service To maintain confidence in the standards, we recommend having validation plates recertified at one-year intervals. Validation plates sent to us are disassembled, cleaned, calibrated, recertified according to ISO 17025, and returned with a new certificate of calibration. \[The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers.\] Ensure ongoing compliance for your Molecular Devices microplate readers and be audit ready with our Installation Qualification, Operational Qualification, and Preventive Maintenance (IQ/OQ/PM) services. # Installation Qualification (IQ) Verifies and documents that all necessary components required for operation are received and properly installed in accordance with Molecular Devices operational specifications. # Operational Qualification (OQ) Tests the mechanical, electrical, and optical components of each instrument to verify proper operating functions in accordance with manufacturer specifications. # Preventive Maintenance (PM) Ensures each instrument meets operational specifications through comprehensive, multipoint inspection. The instrument is calibrated, inspected, and lubricated. Potential issues are proactively addressed. | | | | | | --- | --- | --- | --- | | Certificate of Calibration Number: | | XXXX | | ABS Test results | | | | Test Description | Test Specification | Testestsate | Testeste | | Photomtica | | PASSES | PASSES | | | | | | | Photometric Linearity | goiCeriee.obinthe standardrangeof PD20toPD100 | PASSES | PASSES 回 | | Wavelength Accuracy | Measured Peak=Certiicate Peak+/-1nm | PASSES | PASSES | | Stray Lightat220nm | MnimumOD>=2.5 | PASSES | PASSES 回 | Example of document to evaluate if the reader performs within its specifications. # Key features ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/3e2efd3de35064507c04d08002895b6d120486a3f8c4d18e439f515a82040be4.jpg) Easy operation # Microplate washers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4edf3297e6fc157effec4efea65e48f8b64d68222085c83c86367a70f709f73d.jpg) Minimized wash time ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e29f91e5196ace6540bfd32eba505a9821d652d201096f8f9acc59cc315f1728.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/18c73783a7a5479cbaff3ec218b839dbc0910023d034d23b335d215565758d3e.jpg) Reduced residual volume Patented cell wash head to gently wash cells ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f6d300895da6d52d58119dd938be1b29edeefc2e7cea17ff3002471d0fc26456.jpg) AquaMax® Microplate Washers Microplate washers for biochemical and cell-based assays ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/d5d7a677b55a7e7b53361376d2a11f0560f345c9ecd759dd860f26c23f10ba13.jpg) MultiWash™+ Microplate Washer 96- and 384-well automated washer • Interchangeable 96- and 384-well wash heads • Dispense entire plate at once for fast additions • Completely programmable via touchscreen interface • No external pumps or computer • Comprehensive, automated cleaning utilities • Robotics-friendly design • Low residual volume saving precious and expensive buffers • Walk-up usability • Start simply with wash and rinse bottles (included) • Liquid handling one column at a time • Flexible system washes 96- or 384-well plates • Automated rinse reduces clogging • Quiet and efficient • Low residual volume saving precious and expensive buffers # Microarray scanners ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/81fa9804fc1b5e67297dd53ded4c73c5ea0acb24049788b62842db88b0476b80.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/87bc1436f1284c02b049f36e8eeb78bef9627577f1549c87b8eff1cce6a29fb1.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a30b72467bd2a777eeb750413105037315478f9105b89542d3fd36b56926eb2f.jpg) # GenePix® 4300/4400 Microarray Scanner GenePix® 4100A Microarray Scanner # GenePix® Pro Microarray Image Analysis Software High-resolution, high-quality imaging for up to four-color microarrays Affordable, highquality imaging for two-color microarrays The industry standard in microarray image analysis • High-resolution imaging • Ultimate sample compatibility • Houses up to four lasers • 16-position emission filter wheel • Fully integrated with GenePix Pro Software • Compact, affordable, and easy to use • Superior imaging accuracy • Outstanding reproducibility • Flexible fluorophore collection • Fully integrated with GenePix Pro Software • Multiplexed image acquisition • Powerful automated spot-finding algorithms • Walk-away batch analysis • Automated quality control flagging • Import/export of industrystandard file formats • Full control of GenePix SL50 slide loader • Integration with GenePix scanners and Acuity® informatics software # Key features # Imaging systems High quality images ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5810e0597f7a403d934ad1bfbb5d7d4652eb15c1444d26206c8a457b7aec7101.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1edf2616ddbffc318228cd6945c74de5cc0700bb844f85bd20a965f26cb729bf.jpg) Multiple imaging modes 3D imaging and analysis # ImageXpress® HCS.ai High-Content Screening Systems Turnkey applicationmodules The ImageXpress® HCS.ai systems feature a modular design and a wide array of configuration options, allowing for customization to meet your current needs and on-site upgrades as your requirements evolve. Ease of use Environmental control • Upgraded MetaXpress® Acquire Software for easier, faster image acquisition • AI-powered IN Carta® Image Analysis Software seamlessly integrated into your workflow • Exceptional sensitivity with a $9 5 %$ peak quantum efficiency sCMOS camera • Easy switching between widefield mode and up to four confocal disk options with AgileOptix™ Spinning Disk Technology • Six objective turret alongside optional magnification changer giving up to 12 effective levels of magnification • Configurable system with a simple upgrade pathway to match your current and future research needs Automationcompatible ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f3c85f13fee701a49d4b8a02feb590b67bafd4083a147519dc791682e89a5d44.jpg) # Imaging systems ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/865b9fd4ae64d6c2ba43b0ba5d02318dc6e5f47c1fb9ef4d47a34620172e64f1.jpg) ImageXpress® High-Content Imaging Systems High-content imaging solutions for widefield and confocal imaging # ImageXpress Confocal HT.ai Capture large 3D organoid and spheroid images with up to double the speed # ImageXpress Micro Confocal Combines speed, sensitivity, and resolution for confocal imaging # ImageXpress Micro 4 Configurable, high-throughput widefield imaging for fast biological processes • All units can be upgraded with environmental control and on-board fluidics ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5ad8ceba34f580791c9a077d4831e7fb1b10fca9e3b12153173a311413fd99f2.jpg) # ImageXpress® Pico Automated Cell Imaging System Compact system that allows users to go from samples to results in minutes • Ideal for cell counting, transfection efficiency, and cell health assays • $2 5 +$ preconfigured application protocols • 3D z-stack acquisition • On-the-fly analysis • Tablet and touchscreen compatible • Access data from a browser—anytime, anywhere • Leverage IN Carta for complex image analysis problems ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a54017341e13256759da855188aeb92740afd48a2b86e2b2fad2cfb7795a8413.jpg) # Key features # High-throughput cellular screening ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c97872ac2e8a5f053ea67b6f7f8a3272c597bf0aacadea5379c07b3452eac58c.jpg) Complex event analysis with ScreenWorks Peak Pro 2 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f22f991bc4679ae7e2576c26a594ffe8a4d862364b7663b60f5ba21a141ae8c2.jpg) Configurable optics to suit your needs ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5184e05909dcc549c67f1d859c5c44b404d44f13eaa6c9940f300f8806048149.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c792e59d87e32390fbd905c62f7fe8dee8fcdaa4e389f443b70caec44ceb326d.jpg) User-changeable pipette modules to accommodate 96, 384, or 1536 Customizable automation options • Assess toxicity effects—The HS-EMCCD camera option allows for up to 100 measurements per second, providing detailed information about cardiomyocyte and/or neuronal oscillation. Combined with the ScreenWorks® Peak Pro 2 software analysis module, compound-induced pro-arrhythmic effects such as EAD-like events can be easily identified and flagged. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/02f61f83892c8cac872d46f6fdda4018b0a848b1cdfdb2b58f3b39d8b9296ea7.jpg) FLIPR® Penta High-Throughput Cellular Screening System • Identify lead compounds—With seven LED sets, many filter options, and fluorescence or luminescence detection, the FLIPR system supports many assays, including calcium flux, potassium, and membrane potential. High-throughput kinetic screening for toxicology and lead compound identification • Configure to your throughput— The FLIPR Penta system can be configured to match user needs. From manual assays to automated solutions, to measuring 96-, 384-, or 1536-well samples at a time, to using wash or no-wash assay kits in adherent or suspension cell mode, the system can be upgraded as needs change in the future. # Microbial colony picking ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/b973d046e868cab48bdc89b64800c37b4b1c4f0dac0442e937ac562622707518.jpg) QPix® 400 Series Microbial Colony Pickers Automated microbial clone screening and library management system • Use the QPix system for synthetic biology, DNA assembly, antibody discovery, protein engineering, and phage display workflows • Streamline your workflow with scalable automation – pick up to 30,000 colonies per day • Electronic data tracking for well-documented data control • Sterile environment with customizable HEPA filtration options • Available with high-resolution single-cell imaging capability on day zero. Automatically screen and pick clones that are both high producing and monoclonal—all in one system.\* \* Price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause an adjustment to standard performance. Custom solutions are subject to Molecular Devices Custom Products Purchase Terms. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/fcd22aa3a7ba8724774717233016e779b6fd5c5942e29c2e77af64543fefb18c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/cce88d0973a7f09cb5268f441878372ed102035161f5879e54be5ce78cd18b14.jpg) # Key features # Single-cell isolation ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/2d7f188dbf29967524630ffae955444a1e38039c472e115de97308003feb049a.jpg) Proof of clonality High cloning efficiency ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1af38090c205b989ace5fb030c06cd8b12872514c314d2513262866a3458db31.jpg) • High precision and accuracy in single-cell dispensing, adjustable cell concentration and dispensing volume, compatibility with various plate formats, user-friendly software interface, and the ability to handle a large number of cells in a short amount of time. As gentle as manual pipetting Contamination-free DispenCell™ Single-Cell Dispenser • Small footprint fits in the cell culture hood with gentle pressure dispense for cells and software to trace proof of clonality/ monoclonality immediately. • The tool can effectively detect, size, and dispense particles down to the single-particle resolution based on impedance change that depends on particle volume. Compact, automated cell dispenser for fast, easy, and gentle single-cell isolation • It can accommodate a wide range of cell sizes and types, allowing researchers to adjust parameters such as cell concentration and dispensing volume at a gentle pressure of less than 0.1psi to achieve the desired cell density and distribution. # Mammalian screening # Key features ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/bcc8e89e6b8bf0897eeb5140a32c924d1f64696124867f9e47cf15fc9e582e4d.jpg) Verify monoclonality easily: Objective selection, imaging, and data collection streamlines tracking of colony formation from a single cell ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/bf81e1334a47d1b7d389392f0717670fa352cbbb78a896952be3bf82bee113f1.jpg) Sort viable single cells efficiently: High accuracy robotics combined with gentle fluidics-based systems establish viable clones with much higher efficiency ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e521dd9f19f34d008404ff9d932511e92d234eb682b7843f742ad845257c8bd7.jpg) Streamline workflows: Automation with sample tracking increases throughput, allows for more walk-away time, and provides consistent results ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5715bc063dea0c8a9d1bda42f5eae5dced42bd1ac595dc0ce12e5c7f80169313.jpg) Custom automation options\* ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6f12cd9871a626b120af10d876d5dd0527c45ce102f432d135254ef9beeae66c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/eeb529406e93301720e23ed1d99ee547b9511ed9b3d72b82d65551dc0a34acf3.jpg) ClonePix® 2 Mammalian Colony Picker # CloneSelect® Imager and CloneSelect Imager FL Automate antibody discovery and cell line development workflows. Reduce cost by finding your highest producers with fewer reagents High contrast multichannel fluorescent technology that allows for accurate single cell detection and proof of monoclonality at day 0 • Screen 10x more clones than limiting dilution • Increase probability of identifying high-value clones • Condense the workflow into a single solution • Eliminate or recover unstable clones early • Document evidence of single cells and confluency digitally for auditing and submission to regulatory authorities. The Monoclonality Report is an audit-ready document that supports filing for an Investigational New Drug (IND) Application with the FDA. (21 CFR Part 312) • Image cells non-invasively at multiple time points to monitor colony formation • Screen using high resolution white light imaging and multichannel fluorescence • Deliver real-time results with on-the-fly analysis • Automation and integration ready ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/0da3abc8ce55157c4f21090666f7c27bfe293aa5d8375d7c9c8bd651bb663e59.jpg) # 3D biology ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/174ee5ee57e567bded132e643f2550bc2d7fa926aca892a13c7e026b2bf466e8.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/015e97fc328ab08689eab43b0bd11d1fc2ffbf3094c6aa146d862ebe5498bf56.jpg) BioAssemblyBot™ 400 (BAB400) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/25f0147480567cdd798a82db364f7648135c378acfce2c5006a72981bea1dd35.jpg) 3D Ready Organoid Expansion Service 3D Ready Organoids Intelligent robot used to build 3D model systems with increased throughput and precision Obtain large numbers of high quality, lowvariability organoids in a convenient assay-ready format Cryopreserved, highly reproducible patient-derived organoids ready for immediate use • Six-axis robotic arm exceeds human dexterity to bioprint, pick up biology, and complete complex assays • User-friendly interface allows you to use existing protocols or customize your own • Ability to 3D bioprint while controlling temperature, pressure, UV exposure, and more • Automate complex 3D biology workflows by precisely dispensing organoids— or bioprinting cells—and seamlessly integrate with ImageXpress • Save time and resources by entrusting your organoid production to our team of experts • Available in frozen vials, ready for immediate use • Get over 10 million organoids in a single batch, facilitating robust, repeat screening assays • Stringent, customizable quality control • Choose from a wide range of intestinal, colorectal cancer and breast cancer organoid lines • Provided in frozen vials, ready for immediate use • Integrated HUB Organoids license for approved proof-of-concept studies • Full protocols for use supplied with each vial. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/45694472c7a55c92a4b67cf461acfcf849ae21a04800da8a4e715a592b26b0bb.jpg) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \* Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom ## MultiWash+ Microplate Washer ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/ac6bf8d3dd4751ebb3ee691da9a6de1fc1b32e6156aa442ca7a3f205cc99bb52.jpg) # MultiWash+ Microplate Washer A 96- and 384-well automated washer # BENEFITS • Walk-up usability Start simply with wash and rinse bottles (included) Flexible system washes 96- or 384-well plates Automated rinse reduces clogging • Quiet and efficient # Introduction MultiWash™+ washer is an automated microplate washer that provides maximum flexibility with a minimum footprint. The MultiWash+ washer can be configured for both 96- and 384-well plates and comes with four wash/rinse bottles and one waste bottle for out-of-the-box use. # Wash protocols & modes The on-board touch panel comes with the option of 20 different wash protocols, including up to 8 cycles within each protocol for walk-up useability and flexibility. Washing variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times and three modes of shaking are available to mix solutions. # Efficiency Efficient washing is achieved with crosswise aspiration, reducing residual volume within each well. The washer has a small footprint and is vacuum and pressure free, with on-board pumps that provide a quiet wash experience. # Mouse/Rat IL-22 Quantikine ELISA using the MultiWash $^ +$ washer A Mouse/Rat IL-22 Quantikine ELISA from R&D systems was used to evaluate the MultiWash+ washer. An IL-22 standard curve was prepared and a sandwich ELISA was performed using the MultiWash $^ +$ plate washer in strip mode to wash the wells. After reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0.984. A control well was set up to verify the accuracy of the standard curve. The standard was within the range described with the kit (data not shown). ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/b661d343aaaab0fbddc428dd8ea97622fd9f4746546baa1d47ae6bc5636fbd17.jpg) MultiWash+ washer can dispense in ${ \\tt5 0 \\mu \\mathrm { l } }$ increments and tubing can be inserted into a variety of containers since pressurized bottles are not required. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/cbcb6c5a1d3225c0069ab083160b29aba740e01460be6eb1031a7c84454a61a0.jpg) ELISA Assay # Contact Us Phone: +1-800-635-5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. | | | | --- | --- | | Technical specifications | | Plate wash time (300 μl) | ·96-well plate,1wash: 1 min.9 sec. ·96-well plate,3 washes: 3 min. 35 sec. | | Dispense volume | 50 -2000 μL in 50 μL increments | | Residual volume | <1 μL per well | | Dead volume | 15.62 mL | | Precision | <5% at 300 μL across the plate | | User interface | 2 X16 characters backlit display keyboard with 5 function keys | | Shaking | 3 speeds | | Microplates | ·96-well plates flat and round bottom (384-well plates with 16-manifold) · Fits plates with standard ANSl SBS footprint | | Power requirement | 90 - 250B,60V auto-sensing, 50/60 Hz | | Instrument dimensions (WxHxD) | ·21× 21× 44 cm, ·8.3"× 8.3"× 17.3" | | Weight | 8Kg (17.8Ibs) | | | | | --- | --- | | Ordering information | Partnumber | | MultiWash+ washer for 96-well plates:4 liquid lines,8 way manifold and tubing,4 wash/rinse bottles (2L), 1waste bottle (2.5L) | MultiWash+ | | Optional 16-way manifold | 5032338 | | Optional 12-way manifold | 5032336 | | Washer Maintenance Kit:2XT2.5A spare fuses,2 cleaning needles,tubing sets for all bottles,cleaning tool for needles, tubing set for manifold,and spare tubing set for manifold | 5032339 | | External Tubing Kit (4-way) | 5032340 | | Wash bottle (2L) | 5032341 | | Rinse bottle (2L) | 5032342 | ## GxP Compliance Solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6618eaec2ac73cfaf7d05ac8837f68349098a79f74bc1a4909a969dea0f5b890.jpg) # GxP compliance solutions for GMP/GLP labs # Assure data integrity and compliance with confidence Molecular Devices is a leader in comprehensive comOpliance solutions with microplate detection systems and software. Combined with validation services and support, our solutions assure data integrity. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7980036318570e9ce506496334609b0cd53bd5166f77ea0ea7f0a18ac4c28952.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/8074cca6de17c8b17b49fe9cc9b166f4a3a01ff5aeba021619e093487e7e07d8.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5722659ddbca0b7f605cf55ff62030d73cb1036b8199edab707a5ddf8f51e64a.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/8a42ac3ccef628dab40a0a430bfb4c784d788059f8adfeae8fe4f8da1f33b3cf.jpg) # READERS & WASHERS Best-in-clasBsesmt-inc-rcolapslsa tmeicrreoapldatersreandde washers forw asllheyrosuforrasllsyaoyurnaesesdays . # SOSFOTFMTMAAXXPRO GxPSSOFTFWTAWRAERE OurOumr omsotsts eseccurre soffttwaareretot oacahicehvieve fulflulFl FDA 21 CFR Partrt111a1nadnd EuEdurdarLaeLxexAnnex 11ccoomplipalinacne.ce. # VATAILOINDPALTAITOENS PLATES tVioanlidplatieosntepsltatthespterfsotrtmhaencpeeorf ormance of myicoruorp lamtiecreoapdlearteusrinegatdraecreuasbilneg traceable materials for reliable results. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/21844bf115a3abcbc09183984c769eb2f544a020698bceeaf44d207f54890ee7.jpg) # IQ/OQ &SEPRMV/ICOEQS SERVICECoSm # INSSERTVAILCLEASTIONSSEofRtwVaIreCiEnsSt # CRESCERTIFICATION oSnEplRateVsIaCreEcSl ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6684ac06cbcc1e19e4af3d5fac0d420573856bf79ef5fea44b5087c79fcb093b.jpg) ComOpQrehensive OEM operation raelaqduerasliafincdatwioasnhaerns.d repair coverage for microplate readers and washers. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c70c8e78fed5f1da22cf3503df305b51c1de91ee832e40da794b0a40a16783dd.jpg) Software installation services veoripfeyratinodnadloscpuecmifiecnat itohnast. required components are installed to opVeAraLtIiDonAaTlI sOpNecSifiEcRaVtiIoCnEs. Validation plates are cleaned, dctaol iyboruatweitdh, anedwrecertified taecocfocradlibnrga iton.ISO 17025, and returned to you with a new certificate of calibration. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/afc91ec9324474a4d2a12c05a860779ca31c0fd99a57074a831b6434ab9f465f.jpg) # VsAerLviIcDe iAsTcIonOduNc eSdEbRy oVuIrCE On-site software validation service is conducted by our certified professional. # Key benefits • Partner with a trusted vendor for compliance solutions • Simplify the process of your compliance journey • Confidently record and report your secure data • Rely on quality service and support before, during, and after compliance events # Microplate readers Our microplate readers have empowered discoveries across a wide range of life science research and are the most widely cited. They are designed to meet your current and future assay needs and offer an unlimited breadth of application possibilities. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/c8015b04af20101d2a3445a591a43745c3a27990b287228a29141607b372ed5a.jpg) # SpectraMax i3x Multi-Mode Microplate Reader # Unlimited breadth of user upgradeable application modules • Multi-mode microplate reader and cell imaging system • StainFree™ cell counting with MiniMax™ cytometer • Unmatched signal strength and sensitivity with Spectral Fusion™ Illumination • Optional western blot detection and dual injectors • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6dd6e446f69c17e73dd33e4437e5994ec3136f026754e082291c380388e69833.jpg) # FlexStation® 3 Multi-Mode Microplate Reader # Precise optics, fluidic transfer, and assay flexibility • Five-mode reader with a wide range of applications • User-definable liquid transfer enables multiple live cell kinetic assays • User-defined pipetting simplifies assay optimization • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/da3f90dae0a136ee8b12ad02b294d9215073753744ddd7f03cd912c65b38da35.jpg) # SpectraMax ABS/ABS Plus Microplate Readers # Fast tunable absorbance detection • Compact design • 96- or 384-well microplate compatibility • Eight-channel optics for fast reads • Robotics compatibility # Additional options ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/2c43a64f53d4bfd6be7bde2390242a3d9ad6b93f0884d0a461476a0581b48831.jpg) # AquaMax® Microplate Washers Microplate washers for biochemical and cell-based assays • I nterchangeable 96- and 384-well wash heads • Dispense entire plate at once for fast additions • Programmable touchscreen interface with admin controls • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/9777c17bfdaab8d6218d74ef0ef4afe8cb4e3dc3fd3ef3fff59fd29dc43eced4.jpg) # SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Multi-user microplate readers with dual injectors • Permission-controlled touchscreen lock for use in regulated environments • Ultra-cooled photomultiplier tube detector for noise reduction • Hybrid optical system on the SpectraMax iD5 reader • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/28ea97c6ff0fb20c8f7676f841715d2e2a749c8fb04ce838be24bc51f4a88dba.jpg) # SpectraMax® M Series Multi-Mode Microplate Readers # 2–5 read modes and triple-mode cuvette ports • Dual monochromator tunability • Automated absorbance pathlength correction • Endpoint, kinetic, spectral, and well-scanning • Robotics compatibility ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/bb4e0edcc50642ae971d17969908463d0cd2a7001b59b8c0e499998737ab0a04.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1ca70babe122a7a58b85f92d9b621fb01d6895745779dbd5f739ea4a120265ed.jpg) # StakMax® Microplate Handling System # Simple, microplate automation • I ntegrates with our microplate readers and washers • Stacks up to 50 plates • Can recycle plates for multiple reads • Robust and reliable # IQ/OQ services and PM/OQ service plans # Installation, operational qualification, preventive maintenance, and repair coverage for microplate readers and washers IQ/OQ services and PM/OQ service plans preserve instrument documentation in a digital and compliant format. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive validation, maintenance, and repair documentation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/77f9fb28f70156140f2c85fe86539dfed3b953c19c48b71a5c71ddecaded579a.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f81f9f9fd79ac186bda1b98eab9386e588ecf434cb111acbfb128dc08faa6276.jpg) # Compliance Assurance Service Plan # Installation Qualification (IQ) Verifies and documents that all necessary components required for operation are received and properly installed in accordance with Molecular Devices installation protocols # Operational Qualification (OQ) Tests every subsystem of the reader or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications Enquire about our available custom service plans. Billable PM/OQ service also available. # Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. Our comprehensive multipoint inspection and testing ensures each microplate reader or washer meets our product operational specifications. # Operational Qualification (OQ) Tests every subsystem of the reader or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications # Comprehensive coverage • Unlimited service by our trained and qualified field service engineers1 • Molecular Devices factory-approved parts • Parts, labor and travel for on-site service covered at $100 %$ • OQ following corrective maintenance • Priority response2 • Annual Operational Qualification with Preventive Maintenance Service # SpectraTest Validation Plates The SpectraTest® Validation Plates provide automated, comprehensive, and traceable validation of microplate reader performance for absorbance, fluorescence, and luminescence read modes. To maintain confidence in the standards, we recommend having validation plates recertified at one-year intervals. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e48b9369fdc82718abfb9f17efe8a6ee383ce51f5b9e8680200b9aa2f3beb472.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4340188c244fbf69cef926a3f50561e5989441b4c52a48fef08f1b11158b4f38.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/f634024f56f430a1c147af384a4ce7fc8226693107f1d72d57f63a11b5344e84.jpg) # Automated for ease of use All test measurements and calculations are handled automatically by the SoftMax Pro ${ \\mathsf { G x P } }$ Software protocols. Should any of the measurement parameters fall outside defined limits, a test failure is reported with the suspect parameters identified. # NIST and NMIs traceability Our validation plates are traceable to National Institute of Standards and Technology (NIST) and National Metrology Institutes (NMIs). # Recertification service Validation plates sent to us are cleaned, calibrated, and recertified according to ISO 17025 (ABS1, FL1, and LM1 plates), and returned with a new certificate of calibration. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/81ea272c61f7bd0fa67bad8fe89b44a15a739ff65df9b9b857061846f2c3d9ca.jpg) # Software SoftMax® Pro ${ \\mathsf { G x P } }$ Software is our most secure software to help you achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Every step is optimized to simplify analysis and reporting to support our microplate readers. # System audit trail • The system audit trail tracks and records all actions for easy identification • Electronic signatures and date/time stamps provide paperless documentation • Filtering information simplifies quality control and handling during audits making data export unnecessary # Microsoft SQL Express database Enterprise-level document sharing with complete control over file access permissions • Windows folder permission system is no longer needed Can be easily upgraded later to a regular SQL database for larger amounts of data Reduced support required from IT departments to store and protect data files # Document workflow and status system • The paperless, document status system maintains data integrity with control over document workflows • Project teams can track documents while they are moving through development, review, release, and usage in a controlled environment # Projects feature • Can quickly create diverse project teams and increase usability • Project team members only have access to projects they are assigned to, eliminating the need for IT to create separate file folders with custom permissions # Predefined permission schemes ${ \\mathsf { G x P } }$ Admin comes with three predefined factory roles: Scientist, Lab Manager, and Lab Tech to accommodate the document release workflow • Any number of additional roles can be created and customized using over 50 permission setting options to accommodate for individual SOPs • Permissions are defined on a per role basis and then assigned to a user within a project Users can have different roles within different projects # Improved auto-export functionality • Data can be exported to a location outside the database. Various file formats are available to support the import into other data collection and storage applications— specifically LIMS or SDMS (Scientific Data Management System) packages • Signed XML files provide state-of-the-art data integrity • Multiple formats can be exported at once • PDF exports provide a convenient way of archiving data outside the database or to create a snapshot # Software installation services Our software installation services verify and document that required components are installed to operational specifications. SoftMax Pro $\\mathsf { G x P }$ Software can be installed on a single computer or on a multi-computer networked environment by our expert technical support or professional services team via remote access, respectively. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/fd42c0bf13522c5792088fc55a2336978ef909644724b2b4335f093326096f40.jpg) # Software validation service Our on-site SoftMax Pro ${ \\mathsf { G x P } }$ Software validation service supports FDA 21 CFR Part 11 and EudraLex Annex 11 guidelines and is conducted by our certified professional. Each step in the process will be carefully planned and executed. # On-site visit • After completion of all tests, we will provide you with a complete data package: Completed & signed SoftMax Pro $\\mathsf { G x P }$ validation document (hard & scanned copy) Results cover page with electronic signature Results template with screenshots of each step I Q report for SoftMax Pro ${ \\mathsf { G x P } }$ Software Copy of the Training Certificate # Key benefits Receive a pre-service consultation led by our validation experts Get comprehensive software validation testing Receive a complete set of audit-ready documents Minimize downtime—leave the validation to us and focus on your research Save over a week of your valuable time\* ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/ac4c729c9e3ba6114a3492fd9253322554d5f87c71b29a60a48c62c8f0130460.jpg) # Ordering information # Microplate reader services # Software validation service | | | --- | | Compliance Assurance Service Plans (PM/OQ) | | IQ/OQservice | | Upgrade to perform IQ/OQ or PM/OQ on the customer computer instead of the Field Service Engineer computer | | On-site warranty upgrade | | As-found service | | PM/OQ billable service (when not under Compliance Assurance Service Plan) | | Additional preventive maintenance services | | GxP software | | Latest version of SoftMax Pro 7 GxP Software Suite includes:1software activation for each user license,GxP Admin Software,software lQ/ OQ validation package,user license certificate,compliance certificate, | | Windows 10 compatible | | Additional user licenseand software activation purchases Software installation service | | · Standard server installation (Applies to initial purchase only) ·Customserverinstallation | | | | --- | | On-site software validation for SoftMax Pro GxP 7.1.1 or higher | | Validationplates&recertificationservices | | SpectraTest ABS2Absorbance Validation Plate | | SpectraTest FL1Fluorescence Validation Plate | | SpectraTestLM1Luminescence Validation Plate | | Multi-Mode Validation Plate | | Cuvette Validation Set | | Validation plate recertification- FL1 | | Validation plate recertification- ABS2 | | Validation plate recertification - LM1 | | Validationplaterecertification-Multi-Mode | | Software maintenance plan | | Annual maintenance and technical support plan for SoftMax Pro 7.1.1 GxP Software or higher (Required for first year of new license purchase) | Contact our experts at [info@moldev.com](mailto:info@moldev.com) or call 1-800-635-5577 for ordering information. Please visit our contact page for regional phone numbers. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. ## SoftMax Pro Support Plans ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4e070cc1a6a77ee31e8da386e3e6cdd29d170d3f303f2bf88af819b82e4d94db.jpg) # Support Plans for SoftMax Pro GxP Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/47947c6d985b3ee604a18a5a312ecf0ec8868b73a05b0eb0e4625c608c667422.jpg) # Molecular Devices is committed to providing you with the best possible support for our products. To fit your needs, we offer software support and maintenance plan options for SoftMax® Pro GxP Software. # Technical support process Our Customer Support team is the initial point of contact for general questions about our software and reporting of any incidents. Support is delivered by a multi-region team staffed by Technical Support Specialists trained to provide a rapid response to your queries and incidents. All reported issues will be triaged accordingly. | | | | | --- | --- | --- | | Action Action Details | | Support Timeline | | Initial | ·Respond to queries and incidents · Escalate complex issues to next level | Within 48 hours response | | Triage | · Issues not resolved through standard technical support interactions progress to triage | One week | | Solutions | · Provide temporary workaround solutions · Develop corrective patches (for SoftMax Pro 7.1.1 GxP Software and higher) | Depends on complexity | # Software maintenance for SoftMax Pro 7.1.x GxP Software and higher versions # Additional paid support and services Product training Protocol building and support Custom coding/scripts System architecture consultation or specification System installation/upgrade assistance Data migration assistance # Services not supported Software virtualization Network data security configuration SQL Server backup for networked environment SQL server administration LIMS and automation support Post installation server migration SoftMax Pro 7.1.x GxP and higher versions | | | | | | --- | --- | --- | --- | | overageforSoftmax Pro GxP Software | Scope | No Plan | Plan | | Software activations | Additional SoftMax Pro GxP Software activations\* per user per year | 0 | 2 | | Plate reader connectivity | Assistance with plate reader connectivity troubleshooting | | | | Software function | · Assistance with software functionality troubleshooting (<5 minutes) · Remote screen sharing | | | | Software application support | · Assistance and guidance on feature implementation and use (5+ minutes) ·Direct access to subject matter expert · Access to software & document updates via SpectraNet | | | | Software updates1\*\* | Access to update installers released during active plan term | | | | Continuity maintenance | Access to service packs released during active plan term | | | | GxP Admin 3.x/7.2 Portal support | Support for: ·Password reset account recovery · Userrole management ·System audit trail configuration | | | | Validation support | Support and guidance for: · Software Validation Package · Deviation and risk management | | | | Installation support | Post-deployment guidance on installation of SQL database and server questions | | | | Advanced formula assistance | Software support for data analysis functionality in existing protocols and formulas | | | | Perfective maintenance | Optimize software performance per recommended server computer specifications | | | | Adaptive maintenance | Guidance for adapting our GxP software to changing operating systems | | | | Priority SoftMax Pro INSIDER² | Priority notification of new alpha and beta testing programs | | | 1Starting from SoftMax Pro 7.1.1 $\\mathsf { G x P }$ Software 2Requires enrollment in SoftMax Pro INSIDER program \* Applies to Multi Computer set up only \*\* Software updates may address potential cybersecurity issues. SMPCARE $\\mathsf { G x P }$ support does not include Validation services. # Software support for SoftMax Pro 7.0–7.0.3 GxP Software Coverage for SoftMax SoftMax Pro 7.0–7.0.3 GxP Software | | | | | | --- | --- | --- | --- | | Pro GxP Software | Scope | No Plan | Plan | | Software activations | Additional SoftMax Pro GxP Software activations per user per year | 0 | 2 | | Plate reader connectivity | Assistance with plate reader connectivity troubleshooting | | | | Software function | · Assistance with software functionality troubleshooting (<5 minutes) · Remote screen sharing | | | | Software application support | · Assistance and guidance on feature implementation and use (5+ minutes) ·Direct access to subject matter expert | | | | Software updates | ·Access to software & document updates via SpectraNet Access to update installers released during active plan term | N/A | N/A | | Continuity maintenance | Access to service packs released during active plan term | N/A | N/A | | GxP Admin 2.x | Support for: | | | | Portal support | ·Password reset account recovery ·User/role management Support and guidance for: | | | | Validation support | · Software Validation Package ·Deviation and risk management | | | | Advanced formula assistance | Software support for data analysis functionality in existing protocols and formulas | | | | Perfective maintenance | Optimize software performance | N/A | N/A | | Adaptive maintenance | Guidance for adapting our GxP software to changing operating systems | | •1 | | Priority SoftMax Pro INSIDER² | Priority notification of new alpha and beta testing programs | | | 1Commercially reasonable effort approach 2Requires enrollment in SoftMax Pro INSIDER program ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/687d60deedc5fd700d38e0c25d17a9d463ed29e43e627b8ae27c9d996c0215a0.jpg) # Technical Support We currently address cases raised to Technical Support via phone, email, and online Customer Support communities. # North America # Europe | | | | | | --- | --- | --- | --- | | Hours of operation Monday to Friday | | | Hours of operation Monday to Friday | | | 07:00-17:00 Pacific Time | | 07:00-17:00 GMT | | | (excluding observed holidays) | | (excluding observed holidays) | | Telephone | +1800 635 5577 (option 3) | Telephone | +44 118 944 8000 | | Email | support@moldev.com | Email | techsupport.eu@moldev.com | Outside of these hours, case information and knowledge articles can still be accessed via our online Customer Support Community. You can also send us an email and your case will be logged in by our case management system and made ready for investigation at the beginning of the next business day. On receipt of an email, an automatic receipt response will be provided with further contact information and a case reference. You will receive an emailed response from one of our PhD level Technical Support Application Scientist within 48 hours. If you require urgent assistance, please call our Technical Support. # To ensure we deal with your case as efficiently as possible, please provide the following information: Basic details: Your name, telephone, and email • Company and location • SMPCareGxP Support Plan Status • Full description of issue and impact to business process Software environment details: • Software license key • Software release version • Installation environment (Prod, Dev, or Test) Hardware environment details: Instrument serial number Firmware version Relevant raw data, files, spreadsheets Screenshot/snapshots of error messages Log files ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/02e29ef71e3eacee4ca1dbc81e3ad202faac8ec64681788da22deb7bd901e259.jpg) # Other resources # SpectraNet Customer Care Portal In addition to our standard telephone and email support services, we provide an Online Support Community, SpectraNet. Here, you will find additional resources to answer your support queries more quickly including: ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/fe75cd274f2330596df6e64d5d39d41c595654dbdd013996cdde472aefa37f9f.jpg) # spectranet customer care portal Case logging, tracking, and management Product, platform, and support documentation and downloads Answers to common product issues in our knowledge base • Newly released solutions with corresponding Release Notes If you have an active maintenance agreement and require access to the Customer Care Portal, please contact our support team. # Professional services For services outside of the scope of your maintenance agreement, our Professional Services team can offer onsite consulting services including: • Implementation consultancy • Upgrade consultancy # Protocol and SOP services In addition, we have consultative services to help with your business process and compliance needs to include: Protocol development Protocol verification SOP template development # Ordering information # Software Plans | | | --- | | Anual maintenance and technicalsupportplanforone userlicenseofSoftMax Pro7.x GxP Software and higher versions | | Annual technical support plan for one user license of SoftMax Pro 7.0-7.0.3 GxP Software | | Software Services | | On-site software validation for SoftMax Pro GxP 7.1.1 Software or higher | | Remote software installation | | Remote software installation for advanced server setup | # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current list­ ing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \* Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, Switzerland and United Kingdom ## SpectraMax i Series ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7d152064867df5cdeb3dccea941e2b2bf67c7a45358b4f02fbe049d9e7d90181.jpg) # SpectraMax i Series Multi-Mode Microplate Readers ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/3575483283b9cdc9eb2b3ef90bf390c57a88e798a02cba5df51ba798bc05114b.jpg) The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. # Which one will you choose? ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/a6e0ca2ed8709c83aac012f850c6ebc947f744cbcb482e310bcd6d8a90d1060d.jpg) # SoftMax® Pro Software Designed to provide the simplicity, power, and flexibility required for advanced data analysis, the software offers over 160 ready-to-run protocols, powerful data analysis options, and a range of data output options • All of our readers come with the industry leading SoftMax Pro Software – the most published microplate reader control and data analysis software. • For customers working in regulated environments, we also offer comprehensive $\\mathsf { G x P }$ solutions to help you assure data integrity and compliance. # The right SpectraMax i Series reader for your research With over 30 years designing and manufacturing imicroplate readers, we are constantly striving to improve and refine our instruments. The SpectraMax i Series readers have a number of unique features that allow scientists to get more reliable results faster than ever before, allowing them to overcome frequently encountered challenges in the lab. # Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Because a PMT needs to be set to a specified gain to reach a specific sensitivity, signals that are below or above that gain will not be measured properly, limiting the flexibility of your assays. The Solution: The full dynamic range of the PMT will be used to measure the lowest and highest possible concentration in the same run. This will save you from diluting samples or reading your plate over and over again. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1ee1f9895386149e63624cd7eae4b47a56bbe1187ed448fc9c45f790867454e4.jpg) # Spectral Fusion\* Challenge: The Xenon flash lamp is a great light source to measure all wavelengths. However, the intensity of the light is reduced in the range of 430–680nm. The Solution: Spectral Fusion compensates this by adding specific LEDs to increase the light intensity and therefore sensitivity of your assay. Because LED light intensity can be regulated, we can set our detector to the highest sensitivity to ensure both your lowest and highest concentration samples can be detected in one run, without the chance of saturation. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/953f768ad035055c5e2c02779daa03275d621fffe44ab7b92a5483082c9c792c.jpg) # Spectral Optimization Wizard Challenge: Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation and emission. This typically has to be done for every experiment, as the optimal wavelengths vary with the chemical environment and instrument design, among other factors. The Solution: The Spectral Optimization Wizard saves you time by automatically finding the optimal wavelengths with the highes signal-to-noise ratio by scanning every possible excitation and emission wavelength combination. # NFC Personalization\*\* Challenge: A single machine may be shared between multiple users in a lab, each with their own applications and protocols. This can mean time wasted spent setting up the machine anew for each user, as well as the possibility of confusion or insecure data. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/0da5856e87c2df49f62281fa2cdb0a389cac014844298f48b1f8aae27f336fe6.jpg) The Solution: Built in near-field communication (NFC) technology means each user can be given a tag which can bring up their own custom protocols and results with a single tab, saving time and reducing the chance of user-error. \*On the SpectraMax $\\mathsf { i } 3 \\mathsf { x }$ reader only. $^ { \* \* } \\mathrm { O n }$ the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. For customers working in regulated environments, we also offer comprehensive GxP solutions to help you assure data integrity and compliance. Whether you’re performing routine assays or looking to expand your research capabilities, we’ve got a solution for you. Not sure where your research will take you? No problem! The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. | | | | | | --- | --- | --- | --- | | | SpectraMax iD3 Multi-Mode Microplate Reader | SpectraMax iD5 Multi-Mode Microplate Reader | Multi-Mode Microplate Readerwith MiniMax 300 Imaging Cytometer | | UV/Vis Absorbance DNA/RNA/protein quantification, ELISA (HRP, TMB,AP, etc.) | | | | | Fluorescence EarlyTox" Live Cell(viability), Tryptophan detection,CyQUANT, Pico/Ribo/Oli Green, NanoOrange, Calcium assays/Cardiomyocyte | | | | | beating, Fluorescence injector assays Luminescence Cell Titer-Glo (cell viability), Dual-LUC reporterassay,MycoAlert, | | | | | ROS-Glo HO2 assay, Flash injector assay, NanoBRET/BRET\* Fluorescence assays requiring specific filters (NIR) | | | | | Fluorescence Polarization (FP) | | | | | IMAP kinase assays TRF | | | | | DELFIA TR-FRET | | | | | HTRF, Cytokine measurement, LanthaScreen,LANCE Western blot detection | | | | | AlphaScreen | | | | | Cell imaging\* | | | | | StainFreeanalysis, Spheroid analysis, Cytotoxicity, Marker expression | | | | | Absorbance injectorassays | | | | | Stand alone touchscreen operation | | | | | NFC personalization | | | | | Temp up to 66C | | | | | Bottom read luminescence | | | | | Hybrid filter and monochromator Ex/Em | | | | | 1536 well plates with cartridges | | | | | User-upgradeable for additional read modes and capabilities | | | | \*with optional MiniMax 300 Imaging Cytometer # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Europe\* 00800.665.32860 Hong Kong +852.3971.3530 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ## SoftMax Pro 6 Software # SoftMax Pro 6 Microplate Data Acquisition and Analysis Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/7aaef539ec605374b4fae2ec010f67efaa99d4b7e86332b059cbe84f03491867.jpg) Workflow from data acquisition to reporting # KEY FEATURES • Over 140 ready-to-run protocols • Supports traditional and unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4effe1b42f8ca12d2cd4a811413f46265ec2d52e9279343b35aca1747362b35c.jpg) # SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 19 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software. SoftMax Pro is designed to provide both the simplicity needed for new users and the flexibility and power required for advanced data analysis. Every step has been optimized, whether you are acquiring data from a Molecular Devices microplate reader or importing data from another source, to simplify your analysis and reporting. # SoftMax Pro Software simplifies data acquisition SoftMax Pro offers a variety of options to initiate the measurement of a microplate, the first step in data acquisition. Whether it’s the selection of a pre-written protocol with built-in data reduction or simply setting your own parameters, starting a microplate read is just a few clicks away. • Eliminate the need to configure the connection between your instrument and computer by letting SoftMax Pro automatically recognize your instrument • Use over 140 pre-written protocols with pre-configured assay parameters and analysis settings to read, analyze, and report without having to become an expert • Acquire the best data for your assay using several read options including endpoint, kinetic, spectral scan, and well scan • Obtain the best signal window for your fluorescence-based assays with the fluorescence spectral optimization wizard • Normalize OD measurements to a 1 cm path length using SoftMax Pro’s patented PathCheck® Technology to avoid inconsistent readings from fluctuations in pipetting • Pre-select standard data reduction settings as defaults within a protocol • Ensure your results are never lost with automatic data recovery ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/5f2b5c1ca524807ed9efcd28b2f8796cc51daf93cfdc508d06e2382cf65d7665.jpg) # Import plate-based data from any vendor SoftMax Pro enables you to import data from acquisition sources other than a Molecular Devices microplate reader. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. # SIMPLE SETUP FLEXIBLE AND POWERFUL • Prepare data in the provided easy-to use Excel import templates • I mport data into SoftMax Pro Software from any non-Molecular Devices microplate reader • I mport XML-based data directly into SoftMax Pro Software for easy automation ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/e7f652a74f813e349b6c03fc4e20314eb8cd551ce39b6f3aba2101ed1b2d9688.jpg) # Flexible calculations and formulas SoftMax Pro provides the most comprehensive source of microplate data analysis features available in a microplate reader software. Whether your need is simple curve fitting or extensive calculations across multiple plates, SoftMax Pro covers the full range of analysis capabilities. • Utilize one of the 19 different curve fit options to fit and graph your data in the best possible way • Apply standards across multiple plates to simplify cross-plate analysis and interpolation • Easily automate common calculations like relative potency, $\\mathsf { E C } \_ { 5 0 }$ , and Z factors • Clone your plate data for multiple data reduction options, enabling you to test different analysis methods • Perform custom, mathematical calculations with the SoftMax Pro formula system # Image analysis SoftMax Pro is capable of analyzing more than just standard microplate data. Acquisition and image-based data analysis may be done with the full set of SoftMax Pro analysis tools. • Easily acquire and analyze images generated from the SpectraMax® MiniMax™ Imaging Cytometer ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1f79d419d0fd17cc666747df44290ee3ebe4f24c76d35a0d1331c16a398a7537.jpg) # STANDARDIZE TO A SINGLE ANALYSIS PLATFORM WITH THE SOFTMAX PRO 6.4.1 IMPORT FEATURE • Count cells with StainFree Technology • Recognize your cell types with supervised machine learning • Easily acquire and analyze western blot images generated from the ScanLater Western Blot Detection Cartridge ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/d13ef4a08f3cdad1bcb525d374242c4ddc3d11ecb8e1abb6e20608bfc050a4b3.jpg) # Publish like a pro SoftMax Pro provides you with the tools needed to get your data ready to publish or report. The flexible data output formats give you multiple options to get the data where and how you need it. • View data in lab notebook format • Print it the way you see it in SoftMax Pro • Create reports your way with customizable notes sections and even include your own logo • Export directly to a .xls $\\scriptstyle \\left( \\mathsf { E x c e l } \\right)$ ) file or to XML for LIMS integration • Easily save as a PDF file # GxP tools for FDA 21 CFR Part 11 compliance SoftMax Pro 6 $\\mathsf { G x P }$ Software provides FDA 21 CFR Part 11 compliance tools in conjunction with data acquisition and analysis for $2 0 +$ Molecular Devices microplate readers. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/9a0d6d0c595ddddf1260fe5f54632202364d8afa6e2ffea3807c328d06e91323.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/4e68b244f7eaf724b6eb669cfa5adea434aafef346985be99eccacdadf28f032.jpg) # Powerful compliance tools Faster, easier validation For laboratories in regulated environments, the SoftMax Pro $\\mathsf { G x P }$ Software facilitates adherence to worldwide regulatory expectations with 21 CFR Part 11 compliance tools. • Apply electronic signatures for verification authorization, and approval of data files • Track and record actions in the Audit Trail • Control user licenses and access for increased security with powerful user management options • Assign user permissions with a granular level of control • Set up multiple administrators to manage separate user groups • Administer user accounts remotely • Lockout log on failures and set additional security features including automatic idle log out and password aging • Allow off-line usage and/or guest access • Host user account files on a TCP/IP server • Simplify and centralize site-license administration • Transfer licenses among users quickly and easily Optional hardware and software validation tools are designed to speed and simplify the validation process by making it easier to demonstrate compliance of data collection and analysis. Available tools include: ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/01cb363c346eada5e1b4b5a11137b8558c8caf011cb01d94173b6c17a2932ad7.jpg) • All-inclusive software validation package for SoftMax Pro $\\mathsf { G x P }$ Software • I nstructions and worksheets for Installation Qualification (IQ) • I nstructions and worksheets for Operational Qualification (OQ) including important curve fits and routine calculations such as parallel line analysis • Details on SoftMax Pro $\\mathsf { G x P }$ Software features for implementation of FDA’s 21 CFR Part 11 compliance requirements • Validation plates for precise optical verification of microplate readers with detailed protocols and calculations • On-site service for IQ-OQ and preventive maintenance by a certified field service engineer that includes: • A complete digital qualification report with calibration certification • Compliance to 21 CFR Part 11 requirements with electronic signatures and date/time stamps • I nstant results with automated calculations built into the protocols for error-free procedure • Preventive maintenance to assure the best working condition for the microplate reader. # System requirements # Minimum requirements • Computer with single-core processor, 2 GHz or faster • Windows 7, 32-bit or 64-bit $( \\times 8 6$ or $\\times 6 4$ ) • 2 GB RAM • 500 MB of available hard disk space • $1 0 2 4 \\times 7 6 8$ or higher resolution display • RS-232 serial port or USB 2.0 port with a Keyspan USB-to-serial adapter • CD drive or external USB drive • .NET Framework $4 . 0 ^ { \* }$ # Recommended requirements • Computer with quad-core processor, 2 GHz or faster • Windows 7, 32-bit or 64-bit $( \\times 8 6$ or $\\times 6 4$ ) or Windows 8, 32-bit or 64-bit $( \\times 8 6$ or $\\times 6 4$ ) • 4 GB RAM\*\* • 1 GB of available hard disk space or more • $1 2 8 0 \\times 1 0 2 4$ or higher resolution display with 32-bit graphics display and 256 MB video RAM • RS-232 serial port or USB 2.0 port with a Keyspan USB-to-serial adapter • CD/DVD drive or external USB drive • Internet connection • .NET Framework $4 . 0 ^ { \* }$ \\* . NET Framework 4.0 is installed automatically by the SoftMax Pro installer if necessary. \\*\\* I f running on a virtual machine, Molecular Devices recommends a quad-core or faster processor with at least 6 GB RAM. For automation, 8 GB RAM is recommended. # SoftMax Pro 6.4.1 Features ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/b14139acb1de2a7980229e06457240292cab230da29ecbb6abccbf73cc704727.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/3983772072b04cf5dceb9df00def6783e9ca10b7b9f4fed4163f29fd09e2261c.jpg) $1 4 0 +$ pre-written protocols\* Chromo-LAL Antibody titration Multi-peak identification Equilibrium/percent binding Michaelis Menten Cell Titer-Glo Parallelism test (Fieller’s Theorem) ELISA FP – anisotropy TRF/HTRF DNA/RNA/PathCheck Bradford/BCA Dual-Glo luciferase Trending with LabSpeed LS … and more! Read settings\* Endpoint Kinetic Spectrum scan Well scan: horizontal, vertical, cross, fill Set multiple wavelengths per read Plate type editor Read partial plates\* Multi-plate and multi-mode\* protocols Real-time kinetic data reporting\* Simulation mode Robotic and automation interface Import LIMS template information Export to a LIMS Automate software functionality Integrate with robots Imaging\* Western blot Whole cell: transmitted light and fluorescence Display data as raw and/or red color map or gray scale View plate data in 3D graph Plate cloning for multiple redu Kinetic reduction\* Set first data point to zero Vmax/time to Vmax Onset time Time at: max, min, ½ max Max/min/max-min Mean Area under curve Slope Custom formula Curve fits No Fit (scatter plot) Linear Semi-Log Log-Log Quadratic Cubic Log-Logit 4-Parameter 5-Parameter 5-Parameter Alternate Cubic Spline Exponential Point-to-Point Rectangular Hyperbola 2-Parameter Exponential Bi-Exponential Bi-Rectangular Hyperbola Two-Site Competition Gaussian Brain Cousens Curve analysis $1 C \_ { 5 0 } / \\mathsf { E C } \_ { 5 0 }$ Interpolation Custom weighting Parallel line analysis Display relative potency an I ntegrated confidence inter Formula syntax helper Imaging\*: cell viewing and cou Format notes sections and add images Custom report layout Set graph fonts and curve colors Export graph as a .png file Export: text, Excel, or XML file Export in plate or list format Print as PDF Autosave: .sda(x), text, Excel, or XML file Cell images\* Western blot images\* ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6fb1fdfd3acb8e91a78065bc1810b5e9a79257e97a00ebfbf03c7e00a8983b6b.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/1ded235efc9f1010a93b9009c058ea93d70c80220934ca19135b966f2fa7e66d.jpg) SoftMax Pro $\\mathsf { G x P ^ { \* \* \* } }$ FDA 21 CFR Part 11 compliance tools Electronic signatures Electronic audit trail File lockdown after statement signing Login and password protected Password aging Account deactivation after login failure Central user account administration Multiple administrators Transfer user licenses Modify user and group permissions Control offline use and guest access File path or TCP/IP server connection Software validation package \\* Applies to select plate reader models \*\* Requires SoftMax Pro Import Feature subscription \*\*\* Requires SoftMax Pro $\\mathsf { G x P }$ software package Custom protocol writing, advanced SoftMax Pro support, and $G \\times \\mathsf { P }$ installation services are also available. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/242d20b746e7db6f407a90d943b30642c852fb1bcdeb9607912fb7c0be06fab8.jpg) Sample ID lists / Plate templates Raw data\*\*: text or XML files (Import templates included) | | | | | --- | --- | --- | | Product | Annual Subscription | Non-Expiring | | SoftMax Pro Standard Software Windows 7/8 compatible | One year subscription to the latest version of SoftMax Pro 6. Includes one activation key which expires after one year. Annual renewal. Part number: SMP6 PROF SUBSCR SMP6 ACAD SUBSCR\* \*For verified academic institutions | Non-expiring license for the latest version of SoftMax Pro 6. Includes four activation keys. Part number: SMP6 PROF SMP6 ACAD\* \*For verified academic institutions | | SoftMax Pro GxP Software Windows 7/8 compatible | three). Tiered pricing is based on number of user licenses | Latest version of SoftMax Pro 6 GxP. Includes tri-fold CD package with GxP Admin,SoftMax Pro 6 GxP,Validation Pack CDs,GxP User License certificate, SoftMax Pro 6 GxP Product Activation Key Sheet,and Compliance certificate. Part number: SMP6X GxP\* User license part number: SMP6 GxP License (3-10) SMP6 GxP License (11-50) SMP6 GxP License (>50) \*Requires purchase of any amount of user licenses (minimum | | SoftMax Pro Importer XLS Requires SoftMax Pro 6.4.1 or higher | One year subscription to enable import of data into SoftMax Pro from an Excel-based template. Includes one activation key which expires after one year. Annual renewal. Part number: SMP.IMPORT.XLS | purchased. Please select one user license part number when ordering. | | SoftMax Pro Importer XLS and XML Requires SoftMax Pro 6.4.1 or higher | One year subscription to enable import of data into SoftMax Pro from an Excel-based template or XML file.Includes one activation key which expires after one year. Annual renewal. Part number: SMP.IMPORT.XLS.AND.XML | | # Contact Us Phone: 800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors Regional Offices USA and Canada +1.800.635.5577 Brazil +55.11.3616.6607 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Germany 00800.665.32860 Japan (Osaka) +81.6.7174.8831 Japan (Tokyo) +81.3.6362.5260 South Korea +82.2.3471.9531 United Kingdom +44.118.944.8000 ## SpectraMax Mini Reader ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/6399f2072253af30aff0ddda547adfdacfea007e67b7727795795f6dc89824ab.jpg) # SpectraMax Mini Multi-Mode Microplate Reader Make your first move with confidence ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/908cc320bc7d82fb2b78513a5de4461c9f7ccd9cbad4bf093b543348832f1c16.jpg) # SpectraMax Mini Multi-Mode Microplate Reader Affordable multi-mode microplate reader with best-in-class data analysis software The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution that gives you exactly what you need when you need it. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on sensitivity or performance. Included SoftMax® Pro Software enables rapid data generation and analysis, helping you achieve more in less time. # Key benefits • Ease of use to save time on training, data generation, and data analysis, for faster results • Superior data analysis with best-in-class SoftMax Pro Software, offering pre-configured protocols for the most commonly used applications • Flexibility with plate types from 6- to 384-well formats; up to three modes of detection measuring absorbance with fluorescence, luminescence, or both # Everything you need to run your most crucial applications quickly and easily Access your favorite applications, including ELISA, DNA and protein quantitation, cell viability, gene expression, and more with a compact, upgradeable microplate reader that does everything you need and lets you add detection modes in the future. The SpectraMax Mini reader provides tunable absorbance, filterbased fluorescence and luminescence, temperature control, and easy analysis and data export with SoftMax Pro Software. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/570fb8d7005a52b8a9847ad09db811600269a4ade04fa4e8cdca3f0a95f8eb83.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/959fdca410620689921ee4c6ce6fcd3dfc17b8a8e44ef7fc1ee20b77239ab1ea.jpg) Key features # Fluorescent DNA quantitation Determine DNA concentrations precisely and accurately over a wide dynamic range ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/23f23c8d8d4f1e82bbbbd27efc036112148db3d7d954d41e2f9bcf1e89ef3cde.jpg) # Cell viability Easily measure cell viability with a luminescence readout ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/b05c75e06f9952b2e71b01dd84e2b4af887f28a07346fdf26f18272f97ecce7b.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/df82a1377e3e683cdc77622f86a10ddbc6d25286209e6ce1f7d7ed35de5ca336.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/685ceb81af3ca803edea96f8b3f2fb6751b41d8a964303480544d514618f9a94.jpg) # Reporter gene assays Assess firefly and Renilla luciferase activity over a broad range of cell numbers and expression levels ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/051297a080af1ff882703bcbc08a47c9a5f2b817be23add2dcfdbcaff276899c.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/d28e69a20d4fd1d6e129b06e36aca035150c7ceaf560fe59b983671d937460af.jpg) # Low-volume DNA quantitation Accurately measure DNA from $2 { \\bmod { \\mu \\updownarrow } }$ in as little as 2 µL with the SpectraDrop™ Micro-Volume Microplate ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/3dd77f9495b51637f0970ef2a356a55600d1a8545af110458e4ffe0a54b79526.jpg) Smaller, faster, quantitation of DNA, RNA, and protein with SpectraDrop Micro-Volume Microplate ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/br/images/de45fe3ab38413a0c87d40452bb2ee21c485c316b7b839e07dab25266a960714.jpg) # Filter Cubes # Standard Filter Cubes | | | | | | | --- | --- | --- | --- | --- | | Filter Name | Fluorophore/assay | Excitation | Emission | Dichroic | | FL-465 | Coumarin derivative (AMC), AF350 | 360/35 | 465/35 405 | | | FL-535 | Fluorescein/FITC | 485/20 | 535/25 | 508 | | FL-595 | Alamar blue | 535/25 | 595/35 555 | | | Optional Filter Cubes | | FL-360 | Tryptophan | 280/20 360/35 | 310 | | | FL-565 | Nano orange/Quant IT protein assay | 465/35 | 565/30 508 | | | FL-635 | Alexa Fluor 594, Texas Red | 590/20 | 635/25 610 | | | FL-680 | Alexa Fluor 647/cy5 | 625/35 | 680/20 655 | | | FL-810 | Alexa Fluor 750/cy7 | 710/50 | 810/40 765 | | # Technical specifications – SpectraMax Mini reader General specifications | | | | --- | --- | | Dimensions (in.) | 15.75 (L)× 12.60 (W)× 13.78 (H) | | Dimensions (cm) | 40 (L)x 32 (W)×35 (H) | | Weight | ≤ 40 Ibs (18 kg) | | Power requirement | External 24V DC power supply compatible with 100-240V AC @50-60 Hz 160W maximum consumption | | Robotic compatible | Yes | # General performance | | | | --- | --- | | Plate formats | 6,12,24,48, 96,384-well plates ANSI/SLAS | | Light source | Two high energy xenon flash lamps | | Reading capabilities | Microplates,cuvettes (via adapter) | | Detectors | Ultra-cooled Photomultiplier Tube and Silicon photodiode | | Shaking | Linear, orbital, and double orbital | | Temperature control | 4C above ambient to 45℃ | | Temperature uniformity | ±1C at 37℃ | | Temperature accuracy | ±2°℃ at37°℃ | | Spectral scanning | Abs | | Endpoint reading | Abs, FI, Lum | | Kinetic reading | Abs, FI, Lum | | Well scanning | Over 20 by 20 | # Absorbance photometric performance Fluorescence intensity performance | | | | --- | --- | | Wavelength range | 200-1000 nm | | Wavelength selection | 1.0 nm increments | | Wavelength bandwidth | ≤5 nm full width half maximum | | Wavelength accuracy | ±2 nm across wavelength range | | Wavelength precision (repeatability) | ±0.2 nm | | Photometric range | 0-4.0 OD | | Photometricresolution | 0.001OD | | Photometric accuracy, linearity | <±0.006 OD ±1.0%,0-3 OD | | Photometric precision (repeatability) | <±0.003 OD±1.0%，0-3 OD | | Stray light | ≤ 0.05% at 230 nm | | | | | --- | --- | | Wavelength range | 250-850nm | | Dynamic range | >6 decades | | Sensitivity (fluorescein) | 1pM | # Luminescence performance | | | | --- | --- | | Wavelengthrange | 300-650 nm | | Dynamic range | >7 decades | | Sensitivity (ATP) | 2 pM | | Cross-talk | <0.1% (white 96-well half-area microplate) | SpectraMax Mini Multi-Mode Microplate Reader—Make your first move with confidence in your results | | | | | | | --- | --- | --- | --- | --- | | Contact Us | Regional Offices | | | Phone: +1.800.635.5577 | | USA and Canada +1.800.635.5577 | | Taiwan/Hong Kong +886.2.2656.7585 | | Web: www.moleculardevices.com | | United Kingdom +44.118.944.8000 | Japan | +81.3.6362.9109 | | Email:info@moldev.com | Europe\* | 00800.665.32860 | South Korea | +82.2.3471.9531 | | Checkourwebsite foracurrent | China | +86.4008203586 | India | +91.73.8661.1198 | | listing of worldwide distributors. | Astria | ## Electrophysiology Solutions Overview # Electrophysiology Solutions: Advancing Basic Research and Drug Discovery ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/1dcbd7d54f456a30f10139d5f59cd7968c4b575493cb4d5ce1a7157fa6c692f5.jpg) # Unleash your brilliance™ ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/85669f98f87668853c8d53bc42745c789c54d8bb3ec510ea0721a703cf6a34cb.jpg) # Push the boundaries of electrophysiology like never before With decades of experience making technology advances in electrophysiology, we have enabled thousands of scientists to publish in high-ranking journals and bring novel drugs to market. With our comprehensive suite of electrophysiology solutions, depth of industry knowledge, and expert Ph.D. trained technical support team, we have earned scientists’ trust and preference, becoming the world leader in electrophysiology. The next landmark scientific discovery is waiting for you; learn how we can catalyze your success. “We consistently obtain quality data. We also have had no issues with the hardware in several years of frequent use. Great overall system. Low noise, high quality data with good software.” – Dr. Andrew Voss, Ph.D. Wright State University using Molecular Devices electrophysiology solutions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/6ea9b96a14afbc85092242c4cf95ac89436cc6bb57dc84b71d45007e5aade9a5.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/23d3fcb5f546100a90c2a369f4c0da9d9f069ba9e68d66c0395912f430a7d60a.jpg) MOLECULAR DEVICES ELECTROPHYSIOLOGY BY THE NUMBERS # Secure grants and expand your tenure with high-quality data Make your research exceptional by using the best patch-clamping solution available. Proven with over 20,000 publications by Nobel Prize winners, university professors, and principal investigators, we empower you to win your next grant. With landmark scientific achievements published in Nature, Science, Cell and other key journals, we enable you to look for your next horizon in science. # Publish your way to a permanent position Generate more results faster with best-inclass patch-clamping solutions for reliable high-quality data. Continually publish with science that moves minds and makes you stand out to secure the permanent position you want. You need data fast? Try our new Axon™ Digidata® 1550B Low Noise Data Acquisition System plus HumSilencer™. Equipped with up to four HumSilencer channels, the system enables recording of multiple cells simultaneously without 50/60 Hz line-synchronous noise, all with the push of a single button. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/36e666ecfd28311d9248c29815078d014ef011d8bf8953c6bc84e3402ef5f653.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/12e0b9fc0324a77ab9f6ac519810d7fbb81f91045899c94c3a64d45f18ff2040.jpg) “The capability of eliminating $6 0 ~ { \\mathsf { H z } }$ noise in the new Digidata 1550A plus HumSilencer is a very useful feature in my research. It helps me remove the $6 0 ~ { \\mathsf { H z } }$ noise that obscures my data in my experiment.” – Dr. Zhao-Wen Wang, Ph.D. University of Connecticut Health Center # Transform structure-activity relationship research with electrophysiology that is easy to use Pioneering structure-activity relationship with electrophysiology data is made simple with up to 16 recordings simultaneously in a single run. Not an expert electrophysiologist? Our IonFlux™ 16 Automated Patch Clamp System offers user friendly instrument operation and data analysis putting electrophysiology data within your reach. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/ca2de8c28565b10c13e8526a8e96af413793b81c4d2aa38168a8455ff26f32fb.jpg) Our IonFlux system offers unmatched flexibility, fluidic sophistication and affordability for ion channel research. Integrating microfluidic perfusion with standard well plates produces an automated electrophysiology system that operates with the simplicity of a benchtop microplate reader. # BASIC RESEARCH APPLICATIONS - Single-channel recording - Whole-cell voltage & current clamp recording - I ntracellular sharp electrode recording - Extracellular field potential recording - Two-electrode voltage-clamp recording - Discontinuous single-electrode voltage-clamp recording - Bilayer ion channel study - Electrochemical study - Nanopore study - Clonal selection - Compound screening # Transform successful library screens to routine so you can focus on what is next Need to identify compound hits quickly? With the highest available throughput of up to 6,000 data points per hour and the lowest data point cost, the IonWorks Barracuda® System is your solution. Perform ${ 1 0 0 } , 0 0 0 +$ compound library screens quickly with assay robustness and system reliability you can afford. Are you screening state-dependent or slowly-acting compounds? Population Patch Clamp™ (PPC) recording gives you robust and reproducible results in long assay windows in excess of 30 minutes with stable ionic currents to support the study of use-dependent and slowly-acting compounds. With best-in-class recording stability and longevity to detect these compounds, PPC technology reduces false negatives and false positives so you can select your active compounds fast and with greater confidence. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/9839fca2255f6664c2cd7d5a9c682d8b4a1fe09c5b005e9207d4ec68d714c2ff.jpg) IonWorks Barracuda wide assay window, in excess of 30 minutes, enables compound identification faster and with greater confidence. # Select the next drug candidate for clinical trials Do you need to study ligand-gated ion channels today and voltage-gated ion channels tomorrow? Understand mechanism of action through structure activity relationship studies to identify the next clinical trial candidates. With our IonFlux™ HT system, you get the flexibility and throughput you require with the ease of use and data quality you need. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/b9e043b8e9d75b28b015f5e84241215ca35b668b43dff7daa7dd0c2332d7f425.jpg) # Have the edge in a competitive contract research market Are you competing for the next drug screen? Cost and ease of use are everything. Yet you want flexibility and uncompromising data quality! Today’s drug screen requires ligand-gated channels, yet tomorrow’s might require voltage-gated assays. How do you choose? With our IonFlux HT system, you get both without the need to compromise, so you can stay focused and win your next contract. # LIGAND-GATED TARGETS SUCH AS - Acid Sensing Ion Channel - N-Methyl-D-Aspartate - Gamma Aminobutyric Acid - Nicotinic α1, α3, α4, α7 - Transient Receptor Potential - Purinergic Receptors # VOLTAGE-GATED TARGETS SUCH AS - Voltage-Gated Calcium Channels - Voltage-Gated Sodium Channels - Voltage-Gated Potassium Channels - Human Ether-A-Go-Go Related Gene (hERG) - Chloride Channels ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/31a69bbe1a0f6c5ee59b7ab06d7a4ed783b9fabedf7d705962fdc36ee3750cb4.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/fe991572c3bf2df7a2f99e36cf7784a745b30109d184863e3d90296560573727.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/334c9f237a678dfbc86c851514d57254d120f8dbfa834215f864448bc16ac4dd.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/83a66d03d97d91a6c5faf0d61acea149d631e58cd5539d472942bffe55900261.jpg) # Axon™ pCLAMP™ Software Our pCLAMP Software is built to be a user-friendly electrophysiology data acquisition and analysis program with versatile features for a variety of usage in patch-clamp experiments. # Axon™ Digidata® 1550B plus HumSilencer™ A low-noise data acquisition system that eliminates 50/60 Hz line-frequency noise in less than one second. # Axon™ Axopatch™ 200B Amplifier Widely accepted as the goldstandard for ultra low-noise patch-clamp recordings, it is the premier amplifier for single-channel recordings. # Axon™ MultiClamp™ 700B Amplifier A versatile and computer-controlled microelectrode amplifier designed for patch voltage-clamp or highspeed current-clamp recordings. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/9e3bdda3f0f60b253bb47ba680c4cd48e1549296509e8db6bc615b34ee945d8f.jpg) # Axon™ AxoClamp™ 900A Amplifier A versatile and computer-controlled microelectrode amplifier designed for twoelectrode voltage-clamp recordings and highspeed current clamp recordings. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/3f8e50d915d30df22670a4c28b515e878691b60d73b7c10634b6e122ea802a47.jpg) # IonFlux™ 16 Automated Patch Clamp System Flexible, fluidic sophistication and affordability for ion channel research. Integrated microfluidic perfusion with standard well plates produces an automated electrophysiology system. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/8332baba750e37f329ee0e2650f229ceca6aec23a4f716f4f7359dbc97c96c17.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/b68426d976136cba5951b4ac92c0a4d64c88cae4321f1dd4bd569e0b2c79c059.jpg) # IonFlux™ HT Automated Patch Clamp System Flexible, fluidic sophistication and affordability for ion channel research. Integrated microfluidic perfusion with standard well plates produces an automated electrophysiology system. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/cns/images/9462cfd8c3134e310b3b46e9827fd685e86f81350a1e84a12f998809169ca98a.jpg) # IonWorks Barracuda® Plus System A high-throughput solution for voltage- and ligand-gated ion channel research for drug discovery screening and safety assessment. # Contact Us Phone: 800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors # Regional Offices USA and Canada China (Beijing) Japan (Osaka) Brazil +1.800.635.5577 +86.10.6410.8669 +81.6.7174.8331 +55.11.3616.6607 United Kingdom China (Shanghai) Japan (Tokyo) +44.118.944.8000 +86.21.3372.1088 +81.3.6362.5260 Europe\* Hong Kong South Korea 00800.665.32860 +852.2248.6000 +82.2.3471.9531 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ## ScreenWorks Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/images/62b3b415c5534eb06093a2f8b306bed0e0e35a3d9591a4066d4cbc44242c9d81.jpg) # ScreenWorks Software # ScreenWorks Software Bring simplicity to your multi-user environment with unique protocols utilizing ScreenWorks® Software on the FLIPR® Penta High-Throughput Cellular Screening System. The software controls equipment intelligence and instrument motion as well as capturing data for analysis and export during highly kinetic assays. It records signal from both standard GPCR assays in fluorescent or luminescent format and up to 100 fluorescent images per second during an iPSC cardiomyocyte fast calcium oscillation assay. The optional, add-on Peak Pro 2 software module enables you to perform advanced peak analysis. It is especially helpful for analysis of iPSC derived cardiomyocyte and neuron calcium oscillation assays. ScreenWorks Software is both flexible and user friendly. During protocol setup, only relevant options are displayed, based on the installed fluidics and optics hardware, making it easy to choose parameters that are right for each assay. Live and information-rich data in 96-, 384-, or 1536-well plate format is displayed during testing. Specific wells can be grouped for analysis or data export purposes. Graphs and tables can be easily copied and pasted into documents, presentations or worksheets, and different reductions can be selected for group statistics, making results readily accessible. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/images/1559ea0c07f1f475de1e1da13fc3c58018fedda03167f3b89e1bf177799711d3.jpg) Screenshot of ScreenWorks Software after a basic GPCR calcium flux protocol has been executed. In analysis mode, data grouping and a four parameter curve fit make it possible to quickly calculate the $\\mathsf { E C } \_ { 5 0 }$ value from the curve. # Intuitive, user-friendly software interface Customize protocols and data processing The FLIPR Penta system utilizes our ScreenWorks software to define and run experimental protocols. Using a drag-and-drop interface, protocols can be easily set up to include: Fluorescent or luminescent read modes Simultaneous transfer of 96, 384, or 1536 liquid or cells in suspension Complex quadrant, multiple aspirations or multiple dispensing liquid handling Single or ratiometric kinetic cell-based reading Tip washing with up to two solvents Cell suspension delivery with automatable cleaning protocols Standard fluorescence or optional aequorin luminescence detection Simple-to-create automation protocols Optional Peak Pro software module with easy signal oscillation anomaly detection ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/images/f2900a7163c927553e09fa0b293529cb172c5ac3bce3290250856305877775f5.jpg) # High-throughput early toxicity screening with optional Peak Pro 2 software module Advanced peak detection and event characterization measurements and analysis functionality are available with the optional Peak Pro 2 software module. This data analysis capability is especially useful with cardiomyocyte and neuronal protocols. The Peak Pro 2 analysis algorithm reduces false detections without the need to condition the raw data using search vectors. The FLIPR Penta system with high-speed camera (HS EMCCD) option, for monitoring calcium flux in GPCRs and ion channels with Peak Pro 2 software module, allows you to measure and analyze patterns of calcium oscillations before and after treatment of human iPSC-derived cardiomyocytes and neurons. Images can be taken at up to 100 times per second. You can analyze patterns using more than 30 various measurements. New safety measures in the drug discovery world, such as the NIH CiPA initiative, have placed added emphasis on early assessment of clinical potential. The instrument hardware flexibility and easy-to-use Peak Pro 2 software module can help you assess and quantitate oscillation patterns consistent with potential cardiotoxicity earlier in the drug discovery process. It is also possible to characterize $\\mathsf { C a } ^ { 2 + }$ oscillation patterns in neuronal cells and spheroids. Panel A shows some of the parameters that you can measure. Panel B shows control and two examples of oscillation patterns. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/images/ffb2e6a37a066aae40846a46f41c9f16692ee4b0a545b11239b5e3fef9d76e01.jpg) Panel A) Calcium oscillation measurements available in Peak Pro 2 software module. Panel B) Calcium oscillations: Control; E-4031: hERG blocker; Dofetilide: oscillation irregularities. # Ordering information | | | | --- | --- | | ScreenWorks Software Site License\* | 0320-0392 | | ScreenWorks Peak Pro 1 software module for ScreenWorks 5.x Software | 5075630 | | ScreenWorks Peak Pro 2 software module\*\* | 5074843 | | ScreenWorks Peak Pro 1 software module for ScreenWorks 4.2 Software and lower | 5016900 | \* The host PC that ships with the FLIPR Penta system is preinstalled with ScreenWorks 5.x Software. One license of ScreenWorks $5 . x$ Software supports five installations, including one for the host PC controlling the FLIPR Penta system, and four for data analysis PCs. \*\*Peak Pro 2 software module is only compatible with ScreenWorks 5.0 Software and above. A 14-day free trial is included with ScreenWorks Software. Please see FLIPR Penta system brochure for additional information: [https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/flipr-penta-high-throughput-cellular-screening-system.pdf](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/flipr/flipr-penta-high-throughput-cellular-screening-system.pdf) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ©2021 Molecular Devices, LLC 8/21 2429A Printed in USA ## MetaMorph Imaging Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/bded1698b1ebb951ec1611ba9e7900f41fbcc9b0decdd77db4ff4342efa47ce9.jpg) # MetaMorph® Microscopy Automation and Image Analysis Software An integrated system for bioimaging ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/09356ab548900d1c3585e5b95141d8bbb23f4655310636f2e18a19047e6bb32d.jpg) # Bioimaging techniques contribute to a growing number of scientific breakthroughs. The MetaMorph® Microscopy Automation and Image Analysis Software from Molecular Devices plays a large role in this revolution. With its image acquisition, processing and analysis capabilities, and complete set of tools for automation, MetaMorph software opens the door for new insights into cellular function. MetaMorph software’s flexibility and versatility make it a powerful system for performing operations such as time lapse, multi-dimensional acquisition and 3D reconstruction, and for making measurements such as morphometry, colocalization and brightness. In biological experiments using live cell imaging, MetaMorph software combines the speed, flexibility, and unmatched customer support required to get better results, faster. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/a1e835c2bfb9eb24f8e2acdd8e1ff80392a3f2fedec9793eec2e1ac9894343df.jpg) # An integrated imaging system for maximized control # Device automation for easy acquisition Custom configured for you MetaMorph software provides high-end control for devices including microscopes, filter wheels, shutters, cooled CCD cameras, video cameras, monochromators, focus motors and Piezo electric focus devices, motorized stages, digital and serial input/output, and robotic devices. # A complement to your confocal system MetaMorph software is a great addition to your core facility’s confocal station. With the MetaMorph Offline package, you can measure, analyze, and display multi-dimensional data acquired from a confocal system. # A variety of applications to suit your needs Developed in conjunction with leading bioscience researchers, MetaMorph software offers tools for imaging applications such as: Multi-dimensional imaging 3D deconvolution 3D reconstruction Colocalization and brightness measurements Particle tracking and motion analysis Fluorescence, FRET, FRAP, and FISH Morphometry Multi wavelengths cell segmentation Neurite outgrowth Angiogenesis tube formation Time lapse Z-series And more MetaMorph software is available in multiple custom configurations: MetaMorph Basic Acquisition for Microscope (Part no. MMACQMIC): Includes Camera Acquisition Option and Complete Microscope Control. MetaMorph Advanced Acquisition (Part no. MMADVACQ): Includes all Acquisition Options and Device Drivers. MetaMorph Premier Acquisition (Part no. MMPREACQ): I ncludes all Acquisition and Control Options and Device Drivers. Includes 5 Optional Application Modules. MetaMorph Premier Plus Acquisition (Part no. MMPREPLU): Includes all Acquisition and Control Options and Device Drivers. Includes all available Optional Application Modules except AutoQuant and Super Resolution Modules. # MetaMorph Offline Packages: The offline packages have any or all of the processing and analysis capabilities depending on the package without hardware control and is perfect for multi-user facilities. Available for Basic and Premier. MetaMorph Network Premier Package: This offline package is available for three seats or more. # A powerful multi-dimensional imaging tool MetaMorph software is optimized for multi dimensional experiments. In addition to $\\mathsf { X }$ and Y dimensions, you can acquire and display: Z-axis or multiple focus series (Z dimension) Multiple fluorochromes (Wavelength dimension) Time lapse (Time dimension) Multiple stage positions (Stage dimension) A simple interface guides you through each dimension and settings can be modified after acquisition is initiated. The microscope peripheral controls are integrated in the Meta software toolbar, displaying current illumination, magnification, and $x \\mathsf { Y } \\mathsf { Z }$ location settings. The software’s customizable auto-focus capabilities keep lengthy time-dependent events in focus. For any multi-dimensional experiment, you can: • Align images within a stack Create a montage Create and play a movie, export as AVI Render a 3D reconstruction Create Z-series projections Color-combine images Measure through all planes automatically Enhance any or all images Deconvolve the images Equalize light Create topographic surface maps Perform arithmetic operations View orthogonal planes Stitch a stack of images Visualize the experiment in 3 dimensions Obtain 3D measurements Antigen-induced PI3K activity colocalized with TCR-CD3 complexes within the nascent immunological synapse and remained mainly synapse associated at later stages despite substantial TCR internalization. T lymphocytes were isolated from 5c.c7 αβ TCR transgenic mice and infected with two batches of retroviruses expressing PH(AKT)-YFP and CD3ζ-CFP. Usually $1 5 %$ of the T cells were positive for the expression of both constructs at the time of imaging (day 6). CH27 B cells had been pulsed with the MCC peptide $( 0 . 4 \\mu \ M )$ and were pooled with transduced T cells. (a) Differential interference contrast (DIC) acquisitions. (b–d) Epifluorescent midplane acquisitions of PH(AKT)-YFP (b), CD3ζ -CFP (c) and their corresponding overlays (d). (e,f) Three-dimensional interface reconstructions of PH(AKT)-YFP (e) and CD3ζ-CFP (f). (g) A ‘close-up’ view of the area of contact at the 16-min time point (white rectangle, far right panel of a) of PH(AKT)-YFP (red) and CD3ζ-CFP (green) and their corresponding overlay. To improve image quality, out-of-focus light was removed from fluorescent image stacks using a blind deconvolution algorithm. The white bar (far left panel of a) indicates object size; the ‘false-color look-up table (bottom right) indicates intensity values for interface reconstructions (high-low representation for PH(AKT)-YFP and fold increase (left margin) over average surface intensity for CD3ζ-CFP). # Continuous T cell receptor signaling required for synapse maintenance and full effector potential ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/ca002c0a2ba1709df2f099ce348b5324f0ef3e7e9c58cfb2a22f6fc01db5651b.jpg) Reprinted with permission from Nature Immunology (2003) 4:749-755. $\\circledcirc$ 2003. Johannes B. Huppa1,2, Michael Gleimer1, Cenk Sumen1,3 and Mark M. Davis1,2 1 Stanford University School of Medicine, Department of Microbiology and Immunology 2 Howard Hughes Medical Institute, Stanford, CA 94305 3 Center for Blood Research, Harvard Medical School, Boston, MA 02115 # Observe changes over time Intensity over time measurements are important in studies such as protein motility, FRAP, FRET, and protein-protein interactions. MetaMorph software facilitates time lapse acquisition by offering streaming as an acquisition option. With the appropriate devices, our patented streaming allows you to acquire at the maximum rate of the camera. Another feature for time lapse is the Live Replay option. With appropriate devices and when viewing live images, you can press a key when an interesting event occurs and capture a stack containing some past history of the event as well as some data after the event happened. # Customization through journaling Journals are sophisticated, customizable, and powerful macros that record and perform a series of tasks without the need for a programming language. The Journal Editor allows you to create functions to simplify system operations, automate acquisition and device control, and sequence events. User-definable taskbars and custom menus make it easy to achieve one-button control of your system. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d0e8023a73c83639abaf3680b39b27735f134cf27c660827b5dfae360925284d.jpg) Single time point of a time lapse series. Dendritic cells were surface labeled with an antibody to MHC class one and DRAC 5 to stain the nucleus. A data stack was collected using MetaMorph software. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/b4e29fab1079849f0f7870f83e6479df5179a692a10f44ac7f076c95d7335beb.jpg) Dendritic cells were loaded with FURA 2 AM and a scrape was made across the field of view using a fine micropipette tip. Following the scrape, the cells were left to stabilize for 10 minutes and then imaged using a Nikon 2000e2 microscope and MetaMorph software. The cells were poked with a micro injection tip. A calcium flux is seen to radiate out from the original cell across the dish and the flux is carried down the tunneling nanotubules. Results show that the flux did not cross the gap where the scrape occurred. # Dendritic cells loaded with FURA 2 AM Simon C. Watkins Ph.D., University of Pittsburgh School of Medicine, Pittsburgh, PA 15213 Images courtesy of Simon C. Watkins Ph.D., Department of Cell Biology and Physiology and Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213. # Plot colocalization and brightness measurements for visual representations While good experiment data can be obtained by analyzing a single fluorescent probe, you often get better results by examining more complex interactions. Colocalization tools provide a higher level of detail, with quantitative data regarding regions of overlap between two fluorescent probes. These tools enable you to graphically represent the intensities of each probe on a pixel-by-pixel basis and calculate a correlation coefficient to give a measure of both positive and negative colocalization. Your data can then be exported to a spreadsheet or text file. # Measure brightness over time Many fluorescence experiments depend on measuring brightness parameters and MetaMorph software excels at providing this type of information. With MetaMorph software, you can log intensity data from selected regions in an image stack or live video image over time and choose which parameters to capture. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c801326b6fa99ed9b9a09778099aa9919a670c66aecf8bfff7cf2d45b2453712.jpg) Counting synapses along the syp-mutant dendrite based on overlaid images of syp and syt immunofluorescence. For the 12-day-old heterogenotypic cell pair shown (A), determination of autapses and heterosynapses along a mutant dendrite is illustrated for the boxed area (B). Autapses are devoid of syp fluorescence and display syt immunofluorescence (green), whereas heterosynapses are positive for both syp and syt immunofluorescence (yellow). Lines were drawn along the dendrites to determine their lengths. $\[ \\mathsf { B a r } = 2 0 \ \\mu \\mathrm { m }$ (A) and $5 ~ { \\mu \\mathrm { m } }$ (B)\]. Note that several fluorescence puncta that appear after immunolabeling for syp in the rhodamine channel (red) do not contain syt. They represent less than $3 %$ of total syp- or syt-positive fluorescence puncta (unpublished data) and have been excluded from analysis. # Synaptophysin regulates activity-dependent synapse formation in cultured hippocampal neurons Leila Tarsa and Yukiko Goda, Division of Biology, University of California at San Diego, La Jolla, CA 92093-0366 Leila Tarsa and Yukiko Goda (2002) Synaptophysin regulates activity-dependent synapse formation in cultured hippocampal neurons. PNAS. 99(2):1012-1016. $\\circleddash$ 2003 National Academy of Sciences, U.S.A. # Algorithms for particle tracking and motion analysis Follow the movement of tagged particles over time such as fluorescently-labeled cell surface molecules, microtubules, nucleic acids, lipids, and other objects with sub-pixel resolution. MetaMorph software facilitates your analysis with features for spatial calibration, point-to-point measurements, automated ime stamping of images and tracking of objects. Measure $\\mathsf { X }$ and Y coordinates, velocity, mean displacement, mean vector length, and more, then plot your measurements onto printable and custom-configurable graphs for easy visualization. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/fa80eeb2eb7ef36ffeff05e7b3ace90e5e987b76528532bd6d23d6807cad9655.jpg) MTs parallel to the leading edge are coupled to the movement of f-actin. (a) Image from Video 3 (available at [http://www.jcb.org/cgi/content/full/](http://www.jcb.org/cgi/content/full/) jcb.200203022/DC1) of Cy2 MTs (green) and Xrhodamine f-actin (red). Boxes highlight the regions in the lamellipodium (lp), lamellum (la), convergence zone (cz), and cell body (cb) that were used to construct the kymographs in (b-e). The long axis of the boxes was tilted to match the trajectory of speckles as determined by watching Video 3. Green arrowheads highlight the parallel MTs being analyzed. (b-e) Dual wavelength kymographs of the regions highlighted in panel a. Green and red arrowheads highlight speckles in parallel MTs and the actin meshwork, respectively. Bar, 10 µms. # Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells Wendy C. Salmon, Michael C. Adams, and Clare M. Waterman-Storer, Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037 Reproduced from The Journal of Cell Biology, 2002, 158(1), 31-37 by copyright permission of The Rockfeller University Press. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c309dade5a25d23f3091ff159e274f9bce7cf327a7c88897881ce4ddc5fccf85.jpg) Sample display of captured data as a graph. # The speed and precision needed for fluorescence Common applications of fluorescent-based methods, such as photobleaching and photoactivation, are providing new insights into protein dynamics and the biological processes they regulate. With a typical system configuration, MetaMorph software easily automates and simplifies the process of acquiring, color-combining, and visualizing multiple fluorophores. Live cell studies, such as Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP), demand the rapid acquisition and low-light level imaging of highly-sensitive, cooled CCD cameras with high quantum efficiency, low noise and fast readout rates. MetaMorph software supports rapid shuttering for illumination control to minimize photobleaching before exposure to the laser light and while monitoring recovery. Maximal temporal resolution can be achieved with cameras that support streaming subsequent to laser illumination. The software is ideal for the analysis of live cell laser illumination experiments. # An ideal tool for FRET Several key features make MetaMorph software a powerful platform for FRET imaging. First, FRET takes place at extremely low light levels and dark current noise must be minimized. The software supports highly-sensitive, cooled CCD cameras with high quantum efficiency (less noise) and fast readout rates. Second, FRET images are taken at different wavelengths. The software makes it easy to handle automated wavelength devices and automatically aligns multiple images. Third, speed is key to FRET experiments and the software meets this challenge with its support for multi-wavelength streaming using appropriate devices. Finally, a FRET-specific dialog box automates the complex arithmetic needed to account for and correct fluorescent background and bleedthrough in your images. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/450c39a31a251d80a99242a3747f79213da777c1ff4179acd88f5b0d26b42f71.jpg) A gradient of Ran-GTP surrounding chromosomes visualized in egg extracts and abolished by the addition of Ran mutants. Scale bars, $1 0 \\mu \\mathrm { m }$ . (A) Fluorescence images of mitotic spindles showing microtubules (MTs) and IYFP, ICFP, and FRET ratio (IFRET/ICFP) signals, and an MT-FRET ratio overlay showing a decrease in FRET surrounding chromosomes in the presence of YRC and an increase in the presence of YIC due to the presence of Ran-GTP. There is a decrease in ICFP in regions where FRET occurs. # sualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts Petr Kalab, Karsten Weis, and Rebecca Heald, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-320 Reprinted with permission from Science (2002) 295(5564):2452-2456. $\\circledcirc$ 2003 American Association for the Advancement of Science. # Count, classify, and measure multiple cell parameters # Modules for segmentation Canned, application-specific analysis modules are available for MetaMorph software: Angiogenesis, Cell Cycle, Cell Health, Count Nuclei/Cell Scoring, Granularity, Live/Dead, Mitotic Index, Monopole Detection, Multi Wavelength Cell Scoring and Neurite Outgrowth Application Modules. These modules provide users with a range of tools to automate processing and analysis of cellular images. No special microscopy or image analysis knowledge is required. Cellular segmentation and measurements are generated without the need for programming. MetaMorph software’s morphometry tools allow you to choose over 100 different parameters for morphometric measurement or classification of cells in monochrome or color images. Measure all the objects in your image or define filters which restrict the measurements to objects that meet specific criteria. Set your preferences to increase the accuracy of the data gathered, such as the exclusion of cells that touch the edge of the image. Four interactive modes allow you to “pointand-click” as you work back and forth between the objects in the image window and data being displayed in a table, histogram or scatterplot. Your data can then be exported to a spreadsheet or text file for further analysis. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3b898cfa6289318ed7b411881194893b506417aa256725e67ee14c1c3598bd9f.jpg) Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter Waltraud G. Müller, Dawn Walker, Gordon L. Hager, and James G. McNally, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Reproduced from The Journal of Cell Biology, 2001, 154(1), 33-48 by copyright permission of The Rockfeller University Press. The amount of transcript produced by the array is correlated with array size. Shown in the top row (a–f) are GFP-GR arrays from different cells fixed at 3 h of 100 nM dexamethasone. The corresponding RNA FISH signals are shown in the middle row and the overlay images in the bottom row. Note that progressive increase in array size (a–f) is accompanied by progressive increase in the RNA FISH signal. This correlation is confirmed by quantitative analysis of 113 cells as shown in the plot at the bottom of the figure. Each point in the plot represents an array, like those in panels a–f, whose total RNA FISH intensity has been measured and plotted as a function of the measured perimeter of the array. Bar, $1 \\mu \\mathrm { m }$ . # Technical summary # Minimum computer requirements • Microsoft® Windows® 7 or 10 • 512MB or more system memory (RAM) (more memory may be required for processing large image data sets) 200MB free hard disk space for program only (image storage requires more space) • 24-bit graphics display # Microscope control options Most popular automated microscope models from manufacturers Leica, Nikon, Olympus, and Zeiss Digital auto-focus • $\\mathsf { X Y }$ stage device control for popular models from major manufacturers Z-axis device control for popular models from major manufacturers Piezo-actuated Z and $\\mathsf { X Y }$ device control Monochromator control for illumination Filter wheel and shutter control UniBlitz® shutters AOTF for laser control Liquid Crystal tunable filters Custom I/O (RS-232 serial and TTL) Custom drivers # Acquisition options • Digital CCD and CMOS cameras, both monochrome and color, including: cooled, full frame, frame transfer, interline, back thinned, intensified and on-chip multiplication gain from major manufacturers Simultaneous acquisition from two cameras or control of an image splitting device for projection of two or four emission wave lengths onto a single camera (appropriate hardware required) Wavelength streaming and/or Z-axis streaming (patent pending) For complete details of microscope control, acquisition, and other supported devices, consult our website at [http://support.metamorph.com](http://support.metamorph.com/). # Support Technical support via phone, e-mail, or online at [http://support.metamorph.com](http://support.metamorph.com/) Electronic documentation For complete details of features and options, consult our website at [www.metamorph.com](http://www.metamorph.com/) # Basic Configuration Standard Features 8-, 16-, 24-, 48-bit image and stack display and processing, including: over 15 morphology operators, arithmetic operations, Fast Fourier Transform (FFT) processing, shading correction, and background subtraction 3D reconstruction FRET Auto expose from digital cameras Time lapse acquisition Spectral scan acquisition Z-series acquisition (with Z-motor driver) 2D deconvolution Morphometry and distance measurements Data logging and exporting Automation through journals and taskbars Customizable toolbars and windows Multi-dimensional acquisition Overlay multi-fluorescent images Colocalization and correlation measurements Premier configuration standard features and option modules can also be purchased individually for Basic configuration # Premier Configuration Standard Features I ncludes all Basic Configuration Standard Features Scan slide to scan and stitch together multiple fields of view Stitching of individual images 4D viewer – 3D measurements Motion analysis and particle tracking Includes a selection of 5 optional modules # Optional modules Custom module analysis Screening acquisition Screening playback Multi-dimensional motional analysis Angiogenesis Cell cycle Cell health Cell scoring Count nuclei Granularity Live/dead Micronuclei Mitotic index Monopole detection Multi Wavelength Cell Scoring (MWCS) Neurite outgrowth Transfluor Translocation # Additional non-standard modules • 3D deconvolution Super resolution # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.3971.3530 Japan (Osaka) +81.6.7174.8331 Japan (Tokyo) +81.3.6362.5260 South Korea +82.2.3471.9531 $\\circleddash$ 2018 Molecular Devices, LLC 11/18 2213A Printed in USA ## ImageXpress Pico System ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/e40abc03daa4cc8310f8b7354cd3df92798026aa040701086863ececd9811d9b.jpg) # ImageXpress Pico Automated Imaging System with CellReporterXpress Software — our unique system advantages Updated April $1 5 ^ { \\mathsf { t h } }$ , 2018 The ImageXpress™ Pico Automated Imaging System with CellReporterXpress® software enables researchers to get better data faster and improve collaborations with peers—anywhere, anytime. Drawing upon extensive feedback from our customers coupled with over 30 years of imaging expertise, the ImageXpress Pico system enables researchers to capture high quality images and garner more relevant, quantitative data. | | | | | --- | --- | --- | | | | Software Feature | User benefit ·Addresses a wide range of cell and bead-based assays | Detailed specification ·System includes over 25 different predefined | | □ Over 25 different predefined analysis protocols | ·Ready-made tools available for fluorescent and transmitted light analysis | analysis protocols including Angiogenesis,Apoptosis, Autophagy, Cell Count, Cell Differentiation, Cell Scoring,Multi-Wavelength Cell Scoring, Endocytosis, Live-Dead,Internalization,Mitochondria,Lysosomes, Mitotic Index, Phagocytosis, Pits and Vesicles, Protein Expression,Transmitted Light Cell Count, Bead Count, Bead Scoring, Transmitted Light Scoring, and Viral Infectivity. · Optional Patented Neurite Tracing module available. Covered by patent Methods and systems for analysis of fibers and branching structures within an image of a sample:WO2014036153A1 CN104813364A, | | □ Click-to-Find feature | ·Allows users to easily tune the analysis algorithms with a few clicks of the mouse | EP2891134A1,EP2891134A4,US9646194, US20150213301 · Click-to-Find feature automatically defines cell analysis parameters after the user clicks on a few | | □Label-free transmitted light cell analysis | ·ldentify unstained cells as objects for cell counting or for reference against stains in transfection efficiency and other experiments. | cells representing the population of interest. · Predefined modules include patented machine learning based algorithms designed to automatically identify objects in bright-field including small cells, large cells,and round objects such as suspension cells or beads. · Patent #s for Method and system for classifying and identifying individual cells in a microscopy image: US20160259963 CN105849274A,EP3063289A1, | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/ccd32d873da1af50deb9218ad79cd3888649e85757b2bbb562d3576d5253f29a.jpg) | | | | | --- | --- | --- | | System Software | | Feature | User benefit | Detailed specification ·The CellReporterXpress software distributes image | | with on-the-fly capability | ·Analyze and re-analyze images quickly and easily ·On-the-fly image analysis means the image measurements are ready when the experiment is finished · On-the-fly image analysis allows you to interrogate | processing jobs to a multi-CPU environment and is built in a scalable server-client approach that can drive image analysis to be faster than acquisition ·Option to expand the number of processing units that are deployed for image analysis | | management | ·Seamless integration of image and data visualization in one software solution ·No limitation to storage locations,enabling perpetual access to data from within the software as storage needs increase over time ·Network access to data ·No database administrator experience is necessary | ·Software is designed to save to and display data and measurements from multiple locations as defined by the end user. ·Locations are available to all users with appropriate software and IT privileges. ·Distributed storage concept allows simple steps to attach and detach the storage and moving data | | □ Comprehensive data visualization | ·Seamlessly access images,measurements and analysis within a single interface. · Drill down from images to cells and extract multi- parametric measurements ·Rapidly zoom from overview to individual cells to focus on data of interest | from one CellReporterXpress system to another. ·Software includes capabilities for cell galleries, heat maps,scatter plots,and bar charts for data visualization. ·Software interface is designed to review plate level measurements,images and cellular data from within a single integrated package. | | □Secure data access | ·Secure data access aligned with institutional password and security policies ·Minimizes need to manage user login and passwords ·Improves the security of the data | ·Compact multi-scale pyramid image format ·Compatible with Microsoft Active Directory ·CRX application supports institution's password policies | | □ At-a-glance sample temperature and environmental control status | ·At-a-glance temperature,CO2, O2,and humidity information on sample area can be displayed on all user interface screens ·Helps assures user their sample is at-the prescribed temperature,COz,O2,and humidity levels prior to starting acquisition ·Allows user to check after acquisition that sample temperature,CO2,O2,and humidity was | ·Temperature,CO2,O2,and humidity of sample area displayed on menu bar on all user interface screens when enabled ·User sets upper and lower sample temperature, CO2,O2,and humidity alert limits ·Temperature,CO2,O2,and humidity levels available for viewing or plotting at the experiment conclusion. | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d4e014d496c432c75e3d080d26714b54cbecfcf88d18dcf2100ccd1901305cfe.jpg) | | | | | --- | --- | --- | | System Software | | Feature | User benefit | Detailed specification | | Browser based software | ·Minimize the time spent installing software on offline analysis stations ·Access your system and data online from across the room,the building,the organization or the Internet. ·Share,present,and collaborate with built-in sharing and remote access capabilities · Shorten collaborative project time with easy sharing | ·Anytime,anywhere access enabled by software operating as a web client allows either instrument control or data analysis from any device running Chrome or Safari on any operating system. ·Icon driven interface for intuitive,linear workflow | | □ Touch Screen compatible | ·Flexible user experience based on preference for software interaction: touchscreen or keyboard and mouse. ·Intuitive and modern touch-compatible workflow | ·Unique touch optimized user interface,supports tablet,laptop and desktop clients | | □ Login-specific user interface views | ·Personalize your user-interface (Ul). · Lock acquisition or analysis protocols to preserve your personal settings | ·Separate user log-ins allow protection of preferences · Choose from collection of themes to work in dark and bright environments ·Connection to and acquisition from multiple instruments allowed by software. · Configure heat maps and number of significant figures in measurements based on individual user preferences | | Continuous and discontinuous time-lapse capability | ·Capture images at regular intervals or discontinuously to detect spatial changes in cells ·Assemble your set of time-lapse images into a movie | ·Continuous or discontinuous time-lapse image capture ·Outputs time-lapse images in MP4 format | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/37fa1d186f4948498b0ff5547fcb7afe111511d0f595530d6b0a4c37375af7d0.jpg) | | | | | --- | --- | --- | | Hardware | | Feature | User benefit ·Wide variety of samples canbe analyzed from | Detailed specification ·Instantly switch modes with no changing of LEDs | | □Brightfield, colorimetric, and fluorescence modes of operation | fluorescently stained, H&E stained,to unstained samples. ·No need to physically change light sources with mode change-all light sources on board. | cubes, | | □Large field-of-view (FOV) | ·Statistically relevant number of cells can be captured in a single FOV ·Faster image acquisition, yielding more data in less time.Reduce the number of missed targets ·Reduce artifact introduction by minimizing the number of stitching interfaces with multiple FOV · View larger objects | ·Greater than 5-megapixel CMOS sensor · Single image FOV with a 10x objective is 1.39 mm² | | □ Seamless tiling and stitching of image | ·Image large fields of cells,fibrous networks,and tissue sections with on-the-fly tiling · Software stitching of multiple fields-of-view for a seamless,accurate image. · Flexibility to analyze large stitched or tiled regions of well or slides | ·System automatically acquires the correct number of images to represent the area of the well or slide user would like to acquire. ·Based on user input system will tile or stitch selected images together to compose your region of interest. · Side-by-side CellMagnifyrm feature allows you to zoom or pan high-resolution images from whole-well | | □ Bright,stable,long lasting light source | ·Short exposure times for increased throughput ·Consistent photon exposure to the sample throughout the lifetime of the light source ·No mechanical shutter for minimal down-time ·Long LED life for reduced possibility for service calls. | · Solid-state light source with active power monitoring and exposure time compensation (patent pending). · Bright LED light source with a life rated by the manufacturer over 20,ooo hours. | | □ Slide overview mode | ·Quickly identify one or more regions of interest on a slide to further interrogate ata highermagnification ·Minimize time spent discovering your sample on the slide. | ·Overview image of a 1x3"slide captured in a few seconds ·Draw your regions to acquire directly over your | | □ Robust Auto-Focus | ·Choose between LED and image-based auto-focus,or LED-only for rapid,robust and reliable focus- determination | ·Three 3 LED and image-based auto-focus methods, and the LED-only based auto-focus method are available | | □Temperature control from Ambient +8C to 40℃C | ·Stable temperature control for live-cell measurements. ·Stable temperature across the plate for consistent cell growth. | ·Capable of maintaining 37C±0.5C at 23C ambient. ·Plate inhomogeneity<1C | ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/5a0038531217efa161cf17ff3167991de8a6060dda61ab6fa157e9303f7792d4.jpg) | | | | | --- | --- | --- | | Hardware | | | | Feature | User benefit | Detailed specification | | □ Wide magnification range | ·Imagevery large organisms(C.elegans, zebrafish) to very small sub-cellular features (e.g. vesicles) with a single system ·Conduct a wide range of studies needed by core labs,on shared equipment and for dynamic | ·With 4x to 63x magnification,this system has the flexibility needed to match the range of resolutions required by most biology labs typically supporting a wide range of projects | | Z - Stack imaging | research programs ·lmage large organisms under optimal focus conditions using fluorescence and/or transmitted light channels | ·Capture up to 51 Z slice images and create a projected image using one of several available projection methods. · Z-slice thickness as low as 0.2um | | □Label-free imaging | · Implement label-free imaging assays · Localize fluorescent signal to cellular morphology and therefore compare fluorophore-independent morphology with fluorescent image overlay ·Measure transfection efficiency | ·Analysis modules combine use of Transmitted light, RGB,and fluorescence segmentation | | □ Fluorophore flexibility | ·iithes with a configured system | ee to DAPI. ·Support for other fluorophores available upon | | □ Small footprint | · Fit the system in the space you have | request. · Footprint of 45.3 cm (H)x 55.1 cm (W)x43.5 (D) cm | | □ Complete integrated solution | ·Use a single point of contact for service for instrumentation | · Complete integrated hardware and software solution from Molecular Devices | | ·Make use of our complete solution for image acquisition,image analysis,data management and data mining from one vendor | · Highly experienced global support team | | □Live cell imaging with environmental control | · Run multi-day experiments while mimicking physiological environment ·Hypoxia experiments are possible with <1% O2 | · Oz Control = 1-15% · CO2 Control = Ambient to 15% | | ·Small environmental chamber maintains steady conditions for several days ·External humidifying column significantly decreases | ·Active humidity controlled to 85% relative humidity | ## ImageXpress Pico System ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/b3bf4d327746c26d8c4a670c1842687a7bf9c1ff75130ad123e5bd231b449637.jpg) # ImageXpress Pico Automated Cell Imaging System Go from sample to results in minutes # Simply amazing. # Better data, better value, clear choice. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Whether running fluorescence imaging or brightfield assays, the automated imager features comprehensive preconfigured protocols for cell-based assays to shorten the learning curve, so you can start running experiments quickly. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1229e72dcf7c70507b7276bfcd1eb5a124df56c12304286ca918018b7387554d.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/cbb92a95ba957f6713e8bfe7942f1de0a28093831fc95a5d36229ac975c6437f.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/2762eb566ce9f5042c4850954dba7f3a97f4d7bccc9d4b1d0834d3337c4f6b8d.jpg) # Get started in a snap With the icon-driven, user-friendly CellReporterXpress® Image Acquisition and Analysis Software, you can capture and analyze images with minimal training. # Do more than cell counting Expand your assays with various preconfigured templates optimized for many cell-based experiments including apoptosis, mitochondrial evaluation, 3D cell models, live cell/ timelapse, and neurite tracing. # Automate imaging affordably Alleviate the hassle of going to the core lab to run your samples. The system’s lab-friendly price allows researchers to afford the convenience of automated imaging and analysis on their lab bench. With options like environmental control and z-stack acquisition, the system can be ordered to fit your research needs. # Intelligent cellular imaging and analysis The ImageXpress Pico Automated Cell Imaging System does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/412c98703bc10aaf9543d44d03437cb3aaaef7cac13763b3681e8a9f049a9ab8.jpg) # Increase resolution with on-the-fly deconvolution Enhance contrast of images during acquisition with the Digital Confocal\* 2D on-the-fly deconvolution option, allowing you to increase resolution and improve assay quality. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/cba051e051d75d9d0f6a1c8542662ecc50a93c9ce1fad754b26db25a1b5e5c41.jpg) # Identify regions of interest quickly and easily Live Preview simplifies identification of regions of interest, letting you pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/4f28c8f77e3186f04c9acfa5bd249f37c661fb2f2f65f7f23e8e9725c83f2576.jpg) # Remove the guesswork with preconfigured analysis protocols CellReporterXpress offers over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis removes the guesswork from optimizing parameters. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/61606d923a3710e537dcc502c45d68d35145eca303ad8442f7090b7f1c3a0d63.jpg) # Monitor live-cell assays with on-board environmental control Multi-day, time-lapse, and live-cell assays can be run using the onboard environmental system with options for humidity, ${ \\mathsf { C O } } \_ { 2 }$ , and $\\mathsf { O } \_ { 2 }$ control. Optimized to prevent z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1e8ad9d288b5a97257de487957cf258c666cbacc16112415f481454a5ccfcb71.jpg) # Capture deeper insights with z-stack acquisition Generate sharper images for more accurate segmentation using z-stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/050253d8d61defd55657c8c653a4339bc69646073e701b5df8bed23bb76eb9e2.jpg) # Live Preview Simplify the identification of ROIs (regions of interest). Visualize your sample prior to acquisitioin using the virtual joystick to pan around the sample, and interactively adjust focus. Live Preview, featuring “click-to-center” functionality, continuously updates the image so you can easily navigate your sample to find the desired field of view. Whether you’re working on 96-well microplates, slides, or $3 5 m m$ culture dishes, Live Preview helps you to quickly and easily focus on what’s important to your research. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/947476e032af7834f0d3ce189ae9a31ae82a556a2b13df147527db33ab3ed28d.jpg) Easily find, zoom, and save regions of interest # Digital Confocal Significantly increase resolution and assay quality with Digital Confocal 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into the ImageXpress Pico’s fluorescent image acquisition workflow allowing you to capture images with higher signal-to-noise data for more precise segmentation and analysis. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/df1dcd185b3d36813b7092a9a4573d187b5be8489f823484cc379ae2b5bbafd7.jpg) Standard widefield imaging # Environmental control Environmental control mimics the cell environment and enables you to run multiday studies, time-lapse, and live-cell assays. With CellReporterXpress software, the ImageXpress Pico Automated Cell Imaging System provides visibility of environmental control settings during acquisition to ensure that the system is running at peak performance during your assay. The environmental control system integrates directly into the rear of the ImageXpress Pico saving precious lab bench space. Easily add water without disrupting your ongoing experiments to support multiday studies. The unique magnetically-sealed environmental control cassette chamber introduces and maintains gas and humidity while reducing gas usage as compared to flooding the entire system. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/a0e431321a825d79cf6aa1558ef8cb34db210a27ae8adea069004ff50108582b.jpg) Integrated humidity column is easily refilled without disrupting ongoing experiments ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/59067783485ac5958bd98192da1cae28f20eb56f9e5d2fc0f45169568b64d0ad.jpg) On-the-fly image deconvolution decreases exposure time while increasing resolution with Digital Confocal Imaging Magnetically sealed environmental control cassette reduces gas usage ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3e527f34443e86f8ea7137100cb8b1139b192a188cf635fa5c4a77e888a5da59.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c23b9cd0c89cde62c977cef4996201139072310d4260519a589e1567badb741b.jpg) Go from samples to results in minutes # CellReporterXpress® Automated Image Acquisition and Analysis Software This clean, easy-to-learn interface help you perform quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling microscopy imaging with slides or microplates. An icon-driven, linear workflow with a range of predefined protocols provides a streamlined user experience. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/44470abb36956fe3076bb8bcb1e1bb6fa73f2ce8f1663b547ce20190421a5e5c.jpg) # IN Carta® Image Analysis Software Guided workflows and scalable batch processing increase productivity and reduce time to answer. Experiments can be set up quickly and computational resources can run analysis of multiple wells in parallel efficiently. Artificial intelligence-driven machine learning helps you leverage more information and increase accuracy in the analysis of high-content screening data to enable new discoveries with confidence. # From samples to results in minutes CellReporterXpress features over 25 preconfigured acquisition and analysis templates optimized to collect the most ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/bb7b79e9f28abdec8a0534eda8863a5bfda2f738cbc2647a4e39b773ffd81229.jpg) Icon driven step-by-step workflow guides you to your first image and data in minutes | | | | --- | --- | | Specifications | | Imaging modes | Transmitted light (brightfield),colorimetric,fluorescence,live-preview | | Illumination | High power LEDs with >20,oo0 hour life | | Objectives | 6 position automated turret with user-exchangeable objectives.Optics by Leica Microsystems: 2.5XN Plan/NA 0.07,FLUOTAR 4x/NA 0.13,10x/NA 0.32,20x/NA 0.40,40x/NA 0.60,63x/NA 0.70 | | Camera | Sony CMOs,5 megapixel | | Channels | Cy5,TRITC, FITC, DAPI, Texas Red, CFP,white light,and RGB | | Stage resolution | 0.625 μm | | Imaging method | Single color, multicolor,time lapse,z-stacking,and Digital Confocal\*2D on-the-fly deconvolution | | Autofocus method | LED autofocus or image autofocus with LED assist | | Supported labware | 6- to 384-well plates and 25 mm x75 mm (1 in.× 3 in.) slides,35 mm culture dishes | | Supported operating systems | Windows 10 (main computer), Windows 10 and macOS (clients) | | Image output | 16-bit TIFF, JPG,MP4 | | Dimensions (in) | 17.8 (H) × 21.7 (W) × 17.1 (D) | | Dimensions (cm) | 45.3 (H) x 55.1(W)× 43.5 (D) | | Weight (kg) | 38 kg (84 Ib) including options | | Power | 100 VAC to 240 VAC, 50/60 Hz,1.6A nominal at 15V,200 Wats Max | | Ambient operating temperature | 18C to 30C (59°F to 86F) | | Temperature control | Ambient +8C to 40C | | Temperature control homogeneity | 37C± 0.5C at 23C ambient | | Gas control | O2 control,1-15% and ambient, CO2 control,ambient to 15% | | Humidity control | Active humidity control. Sample compartment controlled to 85% relative humidity. | # Flexibility to expand The ImageXpress Pico system accommodates various labware options including slide holders, 35mm culture dish holders, and more. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/986e20fbffad62bf8291beb4501e65bf60bc75e4db184c4282e10b9a41563449.jpg) # moleculardevices.com/pico ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/aae3af48e5e8fa2900633183c0c729a657a83546b50f7ba5f61cab5f61487ab7.jpg) To request pricing information, scan the code below: ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/6835e5890e646df1826745b6a5d2f9ede81c462d498661f878aa00121843b441.jpg) The ImageXpress Pico system features optics by Leica Microsystems. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Italy, Netherlands, Portugal, Spain, Sweden and Switzerland ## 3D Image Analysis Module ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/61f1e7d27b057ffffed83efa85ec597b5a08bf59851c1fe065e5426b484a3277.jpg) # 3D Image Analysis Module For MetaXpress High-Content Image Acquisition and Analysis Software # BENEFITS • Simplify viewing and quantification of 3D structures • Quickly access images and analysis results together • Segment and score in 3D volumes Complex assays and three-dimensional cell models better represent tissue biology and cell interactions, making them more relevant for many toxicity and drug screening assays. The ability to use 3D models in a high-throughput, high-content format is a significant benefit for scientists. The 3D Image Analysis Module for MetaXpress® Software is designed to analyze spheroids, microtissues, cells in a 3D matrix, and small organisms that are acquired as a stack of z-planes. The integrated software interface enables researchers to quickly access the images and analysis results together to distinguish differences in spheroid morphology, cell division, apoptosis, or mitochondria shape in a 3D environment. The module reconstructs the z-stack into a 3D object so appropriate segmentation of features can be performed. Analysis begins with segmentation of individual z-planes as 2D images for measurements such as nuclei count, live/dead categorization, and cell scoring. Then objects are connected in space by a user selected algorithm. As a result, nuclei, individual cells, or whole spheroids are segmented and scored in the 3D volume without missing objects or counting any object twice. # A single, integrated 3D analysis solution • Analyze three dimensional images from 2D and 3D assays Study reactions within individual cells in standard monolayer cultures or measure responses in whole small organisms • Segment images using advanced tools Define co-localization, neurite outgrowth, and numerous morphological characteristics of individual cells as separate objects • Convert 2D images into a 3D object with powerful connection rules Segment and score nuclei or individual cells in the 3D volume without missing objects or counting any object twice • Explore data in new ways Rotate, zoom, and overlay images to visually verify segmentation in 3D • Quantify your 3D measurements Measure a number of informative phenotypic readouts that enable screening for drug activity or for toxicity of test compounds • Choose from a rich set of 3D measurements Evaluate volume, $x \\mathsf { Y } \\bar { Z }$ location, distance to neighboring objects, diameter, depth, various intensity measurements, texture, or number of objects • Visualize raw images with segmentation in 3D Generate interactive, high-resolution renderings and see 3D visualization for each step of your module • Render a movie of your 3D images or segmentation masks Export 3D movies for presentation and to share your results • Eliminate transfer issues and delays between acquisition and analysis software Analyze and assess images and results using a single, integrated software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/4b12a553757eb2454c3a2dcc157e29069eb9287435f3872dc4fa4bcb6f141047.jpg) The Custom Module Editor provides tools for sophisticated analysis of both 2D and 3D assays. The 3D analysis functions enable simple analysis of objects in the 3D volume. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/342256229e482c131d31c927765728e18ea681da68256c55e130c2063cbb3400.jpg) Segmentation of a spheroid at different z-planes. Objects visible in different z planes may be analyzed independently or joined together using powerful connection algorithms. # Contact Us Phone: 800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/187a343fccc0a9e4b1cea51e4b8fe3968546475ea7e5fef39c832ceed7bc0052.jpg) Analysis using a four-parameter curve fit to determine dose-dependent effects for selected compounds. The sum volume of live cells/spheroid is calculated within the 3D volume and is one example of a 3D phenotypic readout. ## ImageXpress Micro XLS ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/5cf89590cba29ff00a022ab1e83243bfaab75027658f4307f4bedda08b6725d4.jpg) # ImageXpress Micro XLS Widefield High Content Screening System Imaging with a vision # Molecular ! Devices [www.moleculardevices.com](http://www.moleculardevices.com/) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/235aea9fd5cfba046ab53c54a820f0e47277bcef7132eda426d2c35df9ba1169.jpg) The ImageXpress® Micro Widefield High Content Screening System is the ultimate combination of speed and flexibility in a turnkey solution to streamline the research and discovery workflow. Built on over 25 years of cell-based imaging experience, the ImageXpress Micro System captures research-quality images with the widest range of objective lenses, enabling you to work at the resolution appropriate for your biology, including small organism, cellular or intracellular events. The system’s robust design precisely locates and identifies sub-cellular features, over multi-day time-lapse experiments when required. For researchers looking to push the boundaries of science, the XLS model of the ImageXpress Micro System leverages large field-of-view optics to map macro-structures with minimal tiling. In addition, querying of large cell populations is accelerated three fold, speeding up the characterization of highly heterogeneous samples or identification of rare sub-populations. Combined with mature software, the custom designed ImageXpress Micro System provides a fast and robust platform to translate new discoveries into scientific breakthroughs. # Key Features • One system, unlimited configurations • Accelerate screens to > 50,000 wells per day > > • Expand assay window with 3-log dynamic range > > • One 384-well, one image > > • Reliable assays with < 5% CV of intensities across a plate > > • Complete solution for HCS with integrated software and database packages # High resolution and high dynamic range The ImageXpress Micro System does not compromise your assay's throughput or quality. Offering more data points per image, the XLS model decreases your time to perform cellular- and high-resolution screens using a large field-of-view camera. Assay window and reliability are enhanced with 3-log dynamic range and $< 5 %$ CV for intensities across the plate, making certain your scientific breakthroughs are picture perfect for research publications. ImageXpress Micro Systems feature: • Standard and XLS models\* • Adjustable field-of-view focuses on sample area most appropriate for your assay • Widest selection $( > 2 5 )$ of objectives • 1x to $1 0 0 \\times$ magnification • 0.05 to 0.95 numerical aperture (NA), air • Oil objectives with 1.4 NA available for research imaging • Highly robust 100 nm resolution voice coil-driven X, Y, and Z stages enables: • Capture of intricate sub-cellular features across images • Monitoring of long structures (e.g. neurons) across high-resolution tiled or stitched images • Repeatability during multi-day time-lapse experiments • High image flexibility score (IFS). Note: IFS $\\mathbf { \\Sigma } = \\mathbf { \\Sigma }$ sensor size (4.66 megapixel) x magnification range $( 1 0 0 ) = 4 6 6$ | | | | | --- | --- | --- | | | Standard model | XLS model | | Sensor | 1.4 megapixel cooled CCD | 4.66 megapixel scientific CMOS | | Field-of-view (4x objective shown to scale on 384 well) | 2.2 mm | 3.5mm 3.5mm | | Default light source | 300W xenon light source 340/380 nm (Fura-2) to > 650 nm | Solid state light source 380 nm (DAPl) to 650 nm (Cy5), > 10,000 hour life span. Other options available through customization. | # Seamless tiling ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/dfcfaef3a87186bff9d4667298dfcdfc77dabf3c2b83e7cde9ba1ffa7a69f3f8.jpg) Precise tiling of images possible with $< 1 0 0$ nm stage resolution. A: Image is a $2 \\times 2$ array of images from a standard ImageXpress System. B: Image (red) shows neurite segmentation is not compromised when it spans multiple images. # End-to-end solution for high content imaging • Extensive sample compatibility • Slides to plates • 6 to 1536 wells • Thin to thick plate bottoms • Glass to plastic • Transwell $\\cdot$ Low- to high-profile • A truly end-to-end solution for high content imaging • MetaXpress® Software Application Modules: turnkey solutions for your 100 most common analysis routines • MetaXpress Software Custom Modules: flexible toolbox for advanced assays • MetaXpress Software Journals: powerful macros to analyze unique applications ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3eab3bf88e4c1cd21860f2f4e16b49433d388f2722ea4b679e354ac4fc1021b2.jpg) Fluorescent and brightfield imaging # Digital confocal imaging ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d698e32e9b5d0ec4e422781536614ea996fd7153b14d2b8597dc2acbc14bcdd1.jpg) Left: iCell cardiomyocytes stained with Hoechst for identifying nuclei (blue), MitoTracker Red for healthy mitochondria (red), and a live cell cytoplasmic reagent, Calcein AM (green). Image is taken with 40X plan fluor objective. Right: Slide mounted mouse liver tissue slice stained with Oil Red O (red) and hematoxylin and eosin (purple). Image of U2OS cells stained for nuclei (blue), tubulin (red) and nuclei marker (green) was acquired using ImageXpress Micro System with digital confocal option. | | | | | | | | --- | --- | --- | --- | --- | --- | | Transfluor Assay Settings: 20X Plan Apo. no binning. 2 colors. 384-well glass-bottomed plate | | Model | Image | Field-of- view | Cells/ field | Sites for 70% coverageofwell | Assay quality | | Standard | | 0.45 mm X 0.34mm | 200 | 50 | Pits: Z' = 0.5 assay window = 3 Vesicles: Z' = 0.67 assaywindow=11 | | XLS | | 0.7mm X 0.7mm | 600 | 16 | Pits: Z' = 0.64 assaywindow=8 Vesicles: Z' = 0.69 assaywindow=24 | | | | | | | | | --- | --- | --- | --- | --- | --- | | Cytotoxicity Assay Settings: 4X Plan Apo. 2x binning. 3 colors. 384-well plastic-bottomed plate | | Model | Image | Field-of- view | Cells/field | Sites for 70% coverageofwell | Assay quality | | Standard | | 2.2 mm X 1.7 mm | 5,000 | 2 | Cytoskeletal degradation: Z' = 0.71 assaywindow=4.4 | | XLS | | 2.8 mm X 2.8 mm | \> 10,000 | 1 | Cytoskeletal degradation: Z' = 0.72 assaywindow=4.3 | • Low-resolution, whole-well, three-color cell scoring application • $> 1 2$ million cells/hour $\\bullet > 3 5 , 0 0 0$ wells/day • High resolution two-color assay (e.g. Transfluor® Beta Arrestin Translocation Assay) • $^ { \\cdot } > 1 . 2 5$ million cells/hour • > 50,000 wells/day • Analysis scaling to be faster than acquisition # Unlimited hardware configurations The open modular design of the ImageXpress Micro System allows for a variety of expansion options at the time of purchase or after market. Pick the right configuration and illumination module to suit your application needs. The system can also be integrated with a third-party plate loading robot or with other customized robotics solutions. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c4959e4753ec76e429e9056a7245675ca89a8e690ebcb4e0a10a16134890b636.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/f4515af5765138311c7285854514673a651c485b1b581a2e9f0910d60a23749d.jpg) ImageXpress Micro XLS System with Transmitted Light Option installed. ImageXpress Micro XLS System with Fluidics Option installed. | | | | | --- | --- | --- | | Option | Feature | Compatibility | | Environmental Control | · Multi-day, live cell time-lapse imaging · Select appropriate atmospheric conditions (e.g.5% or 10% CO2) · Temperature to mimic physiological environment (30-40°C ± 0.5°C) · Control humidity and minimize evaporation (0.5 μL/wel/hour for 96- or 384-well formats) | · Phase Contrast Transmitted Light ·Fluidics | | Phase Contrast Transmitted Light | · High contrast imaging where unstained cells are easily viewed or separated from background (4x-60x) · Ideal for non-fluorescent histochemically stained samples · Nikon 100W Pillar Diascopic Iluminator with TE-C ELWD Condenser · 0.3 NA with 65 mm WD and PhL,Ph1,and Ph2 selectable phase rings · Compare fluorophore-independent morphology visualization with fluorescent imaging overlay. | · Environmental Control | | Fluidics | · Single-channel pipettor · Dispense volumes from 3 μL to 200 μL(±1 μL±5%) · 96- or 384-wellformat FLIPR? System pipette tips · Holds two plates for compound addition or media exchange · Optional plate heating | · Environmental Control (required) | Note: all options, filters, and objectives are available at point of sale or as after market upgrades Configuration tables shown in this brochure do not encompass all configurations available. # Technical specifications • Fast laser autofocus • 1x-100x Nikon objectives • 4-position automated objective changer • 5-position automated filter cube changer, configurable for specific fluorescent dyes and histochemical stains • Fully automated X-Y sample stage and Z focus stage with resolution greater than 100 nm • 20" H x 18" W x 27" D, 180 lbs. (not including options) # Contact Us Phone: $+ 1$ -800-635-5577 Web: [www.MolecularDevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our web site for a current listing of worldwide distributors. # Regional Offices USA and Canada +1-800-635-5577 Brazil +55-11-3616-6607 China (Beijing) +86-10-6410-8669 China (Shanghai +86-21-3372-1088 Germany +49-89/96-05-88-0 Japan (Tokyo) +81-3-6362-5260 Japan (Osaka) +81-6-7174-8831 South Korea +82-2-3471-9531 United Kingdom +44-118-944-8000 ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/f4067500796eec477ff4106d6e3194010824334486bd2d75da9826d8b55b47ec.jpg) # Molecular Devices # [www.moleculardevices.com](http://www.moleculardevices.com/) ## MetaXpress Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/2e8929ba21f28ce3108f2591d817a9866670dde7ad15e3f8aded532d7695f6e0.jpg) # MetaXpress High-Content Image Acquisition and Analysis Software # BENEFITS • Meet high throughput requirements with a scalable, streamlined workflow • Adapt your analysis tools to tackle your toughest problems, including 3D analysis • Run routine modules and custom analyses on parallel processors • Schedule automatic data transfer between third-party hardware sources and secure database ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/75df915ca1f49db87bb7428987b18d84d41b3311624075b2e459c48aebfac5b0.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3069d8bc6f0920c28454f8590738fd51ea1788faee71b405863694b0b9b9e09c.jpg) # Accelerate your discoveries Quickly identify and quantify heterogeneous, sub-cellular and multi-parametric responses in diverse biological systems Capture high-resolution images, recognize and segment objects, calculate parameters, and then translate your information into meaningful data. At Molecular Devices, we’re inspired by your creativity to solve research challenges. Our goal is to provide you with the tools that let you build a successful High-Content Screening (HCS) infrastructure. Our MetaXpress® High-Content Image Acquisition and Analysis Software is the cornerstone of this infrastructure. It is built on our industry-leading software that has been providing scientists with insights into the world of cellular function for over 30 years. We took our extensive experience with cellular imaging and analysis techniques and put it all in one package, delivering an HCS solution as unique as your research. # An end-to-end solution for screening your most complex biological questions Creating an intuitive HCS workflow requires many components working in concert. Our MetaXpress software ties together every step seamlessly, from acquisition to data management. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/be411597e13b6d2b685ffc5bc1ef332a6ae023222f5ccbb4c6a7afb3496268db.jpg) # Acquire MetaXpress software offers the precise control you need to acquire images with the best possible quality. • Capture crisp, high-resolution images using a range of objectives from 1x to $1 0 0 \\times$ at various numerical apertures • Maintain robust focus across a range of sample types with our easily configurable laser-based or imagebased auto-focusing system • Easily integrate live cell acquisition and quantify 3D measurements of thick samples or kinetic assays using the acquisition and analysis wizard • Halve your exposure time, or increase assay quality by $30 %$ using the digital confocal option in the acquisition interface ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/f8badd1d0e7776b07ffc99aa0d3f15ed8c243556ec45628db5ca34d8d53ce448.jpg) # Analyze Expedite image analysis and data processing using our MetaXpress software with application modules that allow you to quickly and easily analyze your data. # Modular toolbox Set up hundreds of routinely used HCS assays whether you’re a novice or an expert. MetaXpress software application modules give you an interactive environment to quantify 2D and 3D measurements, including size, intensities, and distance. # Intelligent segmentation All MetaXpress software application modules are equipped with a proprietary adaptive background correction algorithm, which adjusts image segmentation to the local intensity ranges and shape features within and between cells. This capability gives you the most robust segmentation available in an image-based screening system and improves detection on both phase contrast and fluorescently labeled images. Save analysis results at the cell-by-cell and image-by-image levels with segmentation overlays and review them interactively by selecting a cell and visualizing the corresponding analysis data or vice versa. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/84e3e3ef0e1faea08f31ce90231f63aaf45a82253a776785b67c2f5a2d38f6e9.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/a23121040cd5bf994a8cafb6e85708037e8347d171b143c3926f032533b0e566.jpg) # Store MDCStore Data Management Solution and MDCStore Xchange Service provide a seamless solution for storing, organizing, and sharing your valuable data. No extra steps are required to transfer data to MDCStore after acquisition, so you can re-analyze your data without accessing the acquisition system, maximizing the availability of the instrument for further imaging. Save experiment protocols and share them with any ImageXpress or MetaXpress system connected to the MDCStore Data Management Solution to standardize routines in your laboratory or across your organization. Schedule automatic data transfer between third-party hardware sources and your secure database, and export data to 3rd party and OME compatible files. MDCStore Xchange Service can also be set up to automatically transfer images across two designated locations, making it easy to share data in your file format of choice. # Visualize Review your results with ease, minimizing the need to switch between applications by simultaneously viewing image thumbnails and plate heatmaps. Analyze, interpret, and mine data further with AcuityXpress™ High-Content Informatics software. Key features include: • I ntegration into MDCStore Data Management tool for easy access to needed files • Bi-directional image viewer that facilitates data quality control by connecting images to numerical data • Multi-parametric analysis and visualization • Dose response analysis and curve fitting with over 30 different predefined functions ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1ac26547a2fefb623bca36c3fc28d593fc6e803a0ee0c3cc0fd9660fba2ab351.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/756095073d6d61146180e8add6372061a39fabef52752ac7ecae0197d14c70c1.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/636a38a40a183107e2a82903f54db0314f8eb6858ae599fd332f5959604c8483.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/8bf3ce3349ab55a7ad094ee5567fd35a5c766c3df2835cba0627ccf73055cc0d.jpg) # Customize Our solution is scalable and adaptable to fit your ever changing and unique research needs. # Custom module editor Build specific analysis capability by combining a series of subroutines. With our step-by-step, interactive editor, you can create challenging, multistep algorithms such as counting punctae in neurite outgrowths, and then running them in minutes using MetaXpress® PowerCore™ software. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/682aaf12e39195f689d8bd2b831bd8f06c68b5850b6b86f267882797b110b11a.jpg) # 3D analysis Increase the biological relevance of your screening assays with an integrated and seamless acquisition and analysis of 3D data. Simplify quantification of 3D structures with volume, intensity, and distance measurements. # Journals For ultimate customization of acquisition and analysis, journals enable multi-step routines such as whole-well scanning at low magnification and localizing objects of interest. Develop custom journal macros including automatic integration of intelligent hardware control based on your image analysis results. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/19fdb8e13c7dc3bf6f3ad70a5c84d055a630b487775428b80a57cc5063a18591.jpg) # Accelerate The MetaXpress PowerCore software is a distributed analysis platform with a server that divides plate analysis into small processes and distributes them to multiple clients. Each MetaXpress PowerCore software client, running on multiprocessor computers, executes analysis and relays the results to the server. Built-in flexibility accommodates time and 3D acquired images as well as single plane data. The scalable design accelerates your image analysis at no cost to segmentation quality, overlays, or the large number of analysis outputs. When the analysis is complete, the server efficiently saves all data into the MDCStore database, so results are accessible within your laboratory or across your organization. Put your HCS capability into overdrive with MetaXpress PowerCore software. Typical basic research or HCS configurations will expedite MetaXpress software application module analysis times by $5 x - 1 0 x$ —as fast as acquisition. Using our scalable solution, increase speed further by adding more processors. Core facilities or highthroughput imaging environments can accelerate analysis speeds $1 0 \\times - 4 0 x$ to support multiple ImageXpress systems running in parallel at full capacity. Similarly, scale up allows re-analysis of archived images at speeds up to 25-fold faster than acquisition. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/b67d2331f4f7db52517017ff70f20c28308e65bf6eea7015bec4b1277ff664c6.jpg) Analysis faster than acquisition ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/0fe09d8b8a9acbf091a1a78bd5d9a8b489ccfdbe21f6db6fa84a8f191e10bb69.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/307c4bb9a5c1d740e378f5122ee25cb74fc5bb6f3b03835c8d5cf580e13546e3.jpg) # Ordering information Our experienced scientific teams are available whenever you have support inquiries, issues, and requests for applications or technical information. When you’re ready to take your research to the next level, our applications scientists, trained in the latest technological advancements and assay development techniques, will help get you there. # MetaXpress High-Content Image Acquisition and Analysis Software: • MDCStore High Content Data Management Solution (included) • Standard licensing is transferable to the computer of choice • Network licensing allows multiple users to simultaneously connect to software • AcuityXpress™ Informatics Software, data visualization, mining, and hit selection are ready to use upon system installation MetaXpress PowerCore High-Content Distributed Image Analysis software for high-throughput image analysis: • Various licensing is available to satisfy multiple parallel processes # MetaXpress software application modules: • Optional modules can be added to the MetaXpress software license | | | | --- | --- | | Product | Part number | | Hardware control options | | | Digital Confocal option for ImageXpress Micro systems | 5016604 | | External control for screening acquisition | 9500-0024 | | File transfer and scheduling | | | MDCStore Xchange Service | 9500-0159 | | Application modules | | | Custom Module Editor | 9500-0158 | | 3D Analysis (requires Custom Module Editor) | 5047663 | | Curve fitting | 5058772 | | Transmitted light segmentation (requires Custom Module Editors) | 5058774 | | Angiogenesis tube formation | 9500-0016 | | Cell cycle | 9500-0040 | | Cell health | 9500-0035 | | Cell scoring | 9500-0037 | | Count nuclei | 9500-0017 | | Granularity | 9500-0032 | | Live/dead | 9500-0034 | | Mitotic index | 9500-0036 | | Micronuclei | 9500-0045 | | Monopole detection | 9500-0039 | | Multi-Wavelength cell scoring | 9500-0038 | | Multi-wavelength translocation | 9500-0044 | | Neurite outgrowth | 9500-0015 | | Transfluor@ Assay | 9500-0033 | | Translocation\* (includes Translocation-Enhanced\*) | 9500-0014 | | Transfluor HT Assay | 9500-0043 | | Nuclear translocation HT | 9500-0041 | | Cell proliferation HT | 9500-0042 | | All MetaXpress software application modules | 5062036 | # Contact Us Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. Regional Offices USA and Canada China (Beijing) Japan (Osaka) +1.800.635.5577 +86.10.6410.8669 +81.6.7174.8331 United Kingdom China (Shanghai) Japan (Tokyo) +44.118.944.8000 +86.21.3372.1088 +81.3.6362.5260 Europe\* Hong Kong South Korea 00800.665.32860 +852.2248.6000 +82.2.3471.9531 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ©2018 Molecular Devices, LLC 2/18 2021B Printed in USA ## MetaFluor Imaging Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/54d23c50d3009aa4d8ba70590d45ef76648e30b314b4b1d1cc21f39ce4fda128.jpg) # MetaFluor® Fluorescence Ratio Imaging Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/f9aed03233e4b83ebe5758a60f7a21457137add96ad425683113cd7bb9150bb5.jpg) Typical system configuration for fluorescence ratio imaging. # Fluorescence ratio imaging is the monitoring of live cells in which a fluorescent indicator of intracellular ions is introduced. Indicator dyes have been designed to shift their fluorescence excitation or emission spectrum when binding with specific ions. Images are obtained at two different wavelengths, typically matching the absorption bands at the high and low binding conditions. By ratioing the intensities in the images, it is possible to construct a map showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace and monitor the time course of cellular responses. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/ef11c88cb76214826691819436d0c8cd0e5d6ce0a5cb86102e342e90410d5911.jpg) # Device control and system automation The MetaFluor® Imaging System is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data, ratio image, graphs of intensities, ratios and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously. # Custom configuration Toolbars, menus, wizards and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system. With the MetaFluor System, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor or an analysis-only copy of the software. # Device control MetaFluor works with microscopes equipped with epifluorescence illumination. The system includes device drivers for numerous commercially-available filter wheels, shutters, monochromators and high speed filter changers for illumination control. Camera drivers are optional. The MetaFluor system’s camera drivers support acquisition from a wide variety of digital cameras. MetaFluor enables sub-region, binning and analog-to-digital (A/D) selection if the camera allows it. Gain and exposure time can be set per wavelength for acquisition. Streaming can be used as an acquisition option. With the appropriate devices, streaming allows you to acquire a predefined number of images at the maximum frame rate of the camera (patented). # Journaling and task automation Journals are sophisticated and customizable macros that execute many tasks without requiring you to know any programming language. The software’s Journal Editor allows you to create functions to simplify system operations, automate acquisition and device control, and sequence events. User-definable taskbars make it easy to achieve “one-button” control of your system. # Fluorescence ratio imaging applications # MetaFluor is an ideal tool for: Ratio imaging Calcium imaging FRET pH measurements Ion concentration Intensity-over-time MetaFluor provides the flexibility to measure Fluorescence Resonance Energy Transfer (FRET). FRET involves the non-radiative transfer of energy from a fluorophore in an excited state to a nearby acceptor fluorophore. FRET will occur when fluorophores are within angstroms of one another. This technique is used to infer protein-protein interaction and colocalization. # Simultaneous emission-splitting MetaFluor supports multi-wavelength emission-splitter acquisition. The Dual-View™ device option separates the fluorescent image into a set of two or four spectrallydiscrete images and acquires them on a single CCD chip with a single exposure without overlap. Using the TwinCam option, the Dual-Cam™ multi-wavelength emission splitter device is used to project one wavelength to one camera and a different wavelength to a second camera, allowing simultaneous acquisition from two cameras. This allows the measurement of emission-shifted probes (Indo-1, SNARF, JC-1) or FRET-based sensors (CFP, YFP) at very high speeds, without any moving parts. # Ratio imaging Once acquired, the wavelengths are grouped into two pairs of ratiometric wavelengths, and one isosbestic or transmitted-light image. With this arrangement, it is possible to monitor two indicators simultaneously, such as BCECF and Fura-2 for pH and calcium respectively, while also obtaining a brightfield image of cellular morphology. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/75385d9ea9c441620f30f35faad5bcfde9467478bddc989e9f794567e50b2f3c.jpg) Nuclear activation of caspase-3 precedes apoptotic nuclear changes. (A) Ratio images and phase contrast images of NLS-SCAT–expressing cells. HeLa cells were transfected with $0 . 5 ~ \\mu \ g$ pcDNA-SCAT3. Imaging analysis was started 18 h after transfection. (B) Venus/ECFP emission ratio changes of individual cells examined in A. # atio-temporal activation of caspase revealed by indicator that is insensitive to environmental effec Kiwamu Takemoto1,3, Takeharu Nagai2,4, Atsushi Miyawaki2, and Masayuki Miura1 1 Laboratory for Cell Recovery Mechanisms and 2 Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan 3 Laboratories for Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan 4 Structure and Function of Biomolecules, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Corporation (JST), Nittochi 535, Akinono-cho, Nakagyo-ku, Kyoto 604-0847, Japan Reproduced from The Journal of Cell Biology, 2003, 160(2), 235-243 by copyright permission of The Rockefeller University Press. # Powerful real time processing acquisition # Real time processing MetaFluor will perform frame integration or averaging and background subtraction on your image as your experiment progresses. Ratio shifts or ion fluxes are observed immediately, providing instant feedback on your experiment. # Calibration A direct display of intracellular ion concentrations is obtained by using the various calibration options offered; the Grynkiewicz equation (Grynkiewicz et al.,1985) and titration equation for both in situ and in vitro experiments. These calibrations can then be stored for future use. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/588b7a65bdaf85302155ddaf3ffdaf81315d7df93d6fcfbce6092e73a4ca1ef1.jpg) A simultaneous display of multiple wavelengths images and various customizable graphs provide an easy point-and-click interface. When playing back an experiment, clicking on the graph will rewind or fastforward the experiment to show the images that correspond to that location on the graph. CHO cells loaded with Fura-2. Experiment from the Optical Microscopy and Imaging in the Biomedical Sciences short course at the Marine Biological Laboratory, Woods Hole, MA. Courtesy of Lynda Pierini, PhD, Cornell Medical Center, Ken Dunn, PhD, Indiana University-Purdue University, and Professor Colin Izzard, SUNY University of Albany. G. Grynkiewicz, M. Poenie, R.Y. Tsien (1985) A new generation of $\\mathsf { C a } ^ { 2 + }$ indicators with greatly improved fluorescence properties. The Journal of Biological Chemistry. 260(6):3440-3450. # Image analysis and processing Regions of interest can be generated automatically or manually placed on your image to monitor intensity, ratio value or ion concentration. Measurements are then made simultaneously on all the regions of interest and update continuously on a scrolling graph, allowing you to follow dynamic changes as they occur in your living samples. # Interactive graphs A display of multiple graphs gives flexibility in the presentation of your experiment’s data. MetaFluor enables you to click on graph traces to display a readout of the time and data value for the region nearest to the click. The Event Mark function is useful to record when drugs or solutions were added, experimental conditions changed, triggers were received or sent or other events occurred. You have the option to associate a timer and an alarm bell to each event. Additionally, for perfused samples, ambient conditions can be logged and tracked. Each image has an annotation that is saved within the TIFF file format. The annotation will record wavelengthdependent settings. Additional information can be stored in a protocol file. # Export for data analysis If needed, MetaFluor can log and export all measurements to either a text file or to a spreadsheet program such as Microsoft® Excel®. # Compatible with MetaMorph Because MetaFluor saves images in TIFF file format, you can import them into MetaMorph for further processing and analysis. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/17eeb8b22989ec46988c89ad17e614aa9ca641dde3015ac709c557412920b1a4.jpg) In vivo characterization of FLIPglu- ${ \\bf \\boldsymbol { 6 0 0 \\mu } } .$ . (A) Averaged YFP-CFP emission image shows FLIPglu- $6 0 0 \\mu$ in cytosol and exclusion from nuclei (N) and lysosomes (triangles). Emission intensities were higher in thicker layers of cytosol adjacent to the nucleus. Ratio images are pseudocolored to demonstrate glucose-dependent ratio changes. Red indicates high ratio, and blue indicates low ratio. Integration of the ratio over the entire cells was used to quantify the ratio change. Arrows indicate the addition of $1 0 \\mathrm { \ m M }$ sugar. Each graph shows ratio changes for a single cell. (B) Direct addition of $1 0 \\mathrm { \ m M }$ glucose led to a decrease in ratio. Because of continuous external glucose supply, the ratio remained constant. Increasing the external concentration to a total of $2 0 ~ \\mathsf { m M }$ did not cause further changes. (C) Following addition and detection of glucose, external glucose was removed by perfusion with glucose-free solution. The subsequent increase in ratio indicates reversible glucose detection. (D) According to the FRET theory, the decrease in YFP emission is accompanied by an increase in CFP emission. Probably, because of photobleaching, YFP emission decreased. (E) The addition of glucose but not of $1 0 ~ \\mathsf { m M }$ of other sugars led to ratio changes. # In Vivo Imaging of the Dynamics of Glucose Uptake in the Cytosol of COS-7 Cells by Fluorescent Nanosensors Marcus Fehr, Sylvie Lalonde, Ida Lager, Michael W. Wolff, and Wolf B. Frommer, The Zentrum für Molekular Biologie der Pflanzen Tübingen, Plant Physiology, Auf der Morgenstelle 1, D-72076 Tübingen, Germany Reprinted from The Journal of Biological Chemistry (2003) 278, 19127-19133 by copyright permission of The American Society for Biochemistry and Molecular Biology. # Technical summary # Minimum computer requirements • Microsoft® Windows® 7 or 10 512MB or more system memory (RAM) (more memory may be required for processing large image data sets) 200MB free hard disk space for program only (image storage requires more space) 24-bit graphics display # Acquisition • Up to five wavelengths per cycle Real time background subtraction (independent background for each wavelength) Real time shading correction (independent shading reference for each wavelength) Time lapse # Automation Control for multiple shutters, filter wheels, monochromators and other wavelength-changing devices Device triggers for pumps, valves, strobes or flash lamps using TTL outputs Customizable journals and taskbars # Digital camera acquisition features # (depends on imaging hardware used) Exposure time, gain, A/D transfer speed, bits-per-pixe for each wavelength On-chip gain multiplication Binning and sub-region selection Control of integrated camera shutter Support for frame transfer, interline, full frame, back illuminated, CCD and CMOS cameras Streaming of data for high speed applications # Calibrations Grynkiewicz equation Titration calibrations with choice of curve fits Calibration maps to directly display pH, calcium or other ion concentrations # Analysis • Ratio of up to two indicators per cycle Automatic generation of multiple regions of interest Fluorescence Resonance Energy Transfer Multiple graphs display Event Marks and image annotation Tracking of experiment conditions I MD, pseudocolor or monochrome display AVI formats for movies Data logging to text file or spreadsheet such as Microsoft® Excel® Compatible with MetaMorph® • Compatible with pClamp® versions 10 and 11 # Custom configuration • Multi-users environment available • Settings storage for each type of experiment # Support Technical support via phone, e-mail or online at support.metamorph.com/metafluor Electronic documentation # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 United Kingdom +44.118.944.8000 Europe\* 00800.665.32860 China (Beijing) +86.10.6410.8669 China (Shanghai) +86.21.3372.1088 Hong Kong +852.3971.3530 Japan (Osaka) +81.6.7174.8331 Japan (Tokyo) +81.3.6362.5260 South Korea +82.2.3471.9531 $\\circleddash$ 2018 Molecular Devices, LLC 11/18 2214A Printed in USA ## CellReporterXpress Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/14f18f1c71731f88dae8cb192cf75ccb10efd945e4377daf6fd739f188f200a0.jpg) # CellReporterXpress Image Acquisition and Analysis Software Easy-to-learn software optimized for automated microscopy using the ImageXpress Pico system # Microscopy imaging for cell counting through to complex image analysis The CellReporterXpress® Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico Automated Cell Imaging System. It has a clean, easy-to-learn interface for performing quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling microscopy imaging with slides or microplates. An icon-driven, linear workflow with a range of predefined protocols provides a streamlined user experience. # Key Features • $2 5 +$ preconfigured application protocols • Automated focusing routines for a variety of cell-based imaging applications • Region selection for acquisition • Label-free analysis • Click-to-find feature • Side-by-side cell magnification • Touchscreen capability • Browser-based software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c3e2f102b40c2c2b6481d3d57c017815972526e07a269b1076bd4e985d55d862.jpg) # Analyze during acquisition Reduce the time to run experiments with our integrated on-the-fly image analysis capability, allowing you to view numeric data during acquisition. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c0a5bcbd16484a7d8eff0d8ac624541c741abd5b7f255e05f07a81132b785473.jpg) # Simplify image analysis Predefined analysis protocols for label-free and fluorescence imaging provides you with the ability to quickly run common biological assays while still allowing you to create and save your own protocols. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/19b880acb4fdbde739235e6342f29ff7d39dacb6c235e439c87ad36c49fbcb6e.jpg) # Share and access data from anywhere The software makes sharing, presenting, and collaborating with peers easier, allowing multiple users to utilize the software simultaneously through browser-based remote access. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/5c3b4d7cf94c2cf6db79d6e8717e9127dd44b55e1191c9553a592adc00dc3fe8.jpg) # Intelligent image acquisition and analysis This software, along with the ImageXpress Pico system, does more than imaging—it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3d88f3618101f8bb52a992c826b60d04143f503ebc421350b6f8f54ef4e85f29.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/01e8ed4d79eda4cc3185c1b521244fa682727c8178285b5c9e14402cf6524781.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d6a21edf0947cce2c92c0babf78f998102b234057c543d1b70d9710c2264314d.jpg) # Increase resolution with on-the-fly deconvolution Enhance contrast of images during acquisition with the Digital Confocal\* 2D on-the-fly deconvolution option, allowing you to increase resolution and improve assay quality. # Identify regions of interest quickly and easily Live Preview simplifies identification of regions of interest, letting you pan around the sample and interactively adjust focus with a virtual joystick, saving time and effort. # Remove the guesswork with preconfigured analysis protocols Over 25 preconfigured analysis protocols ranging from simple cell counting to sophisticated neurite tracing analysis removes the guesswork from optimizing parameters. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/ddc3436629005e9b3c08a68cae6c3b7767dc604fca1baf3bbe227a2159683cfa.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/0c7f39967aaf3d599ffa654e879ed776a5b5d1c706d48d128c1ea3b1f6077dff.jpg) # Monitor live cell assays with on-board environmental control Multi-day, time-lapse, and live cell assays can be run using the on-board environmental system with options for humidity, ${ \\mathsf { C O } } \_ { 2 }$ , and $\\mathrm { O } \_ { 2 }$ control. Optimized to prevent Z-drift, the software also provides real-time monitoring of environmental state, ensuring optimal assay conditions. # Capture deeper insights with Z-stack acquisition Generate sharper images for more accurate segmentation using Z -stack acquisition. Acquire a series of images at different focal points to capture more detail than with a single slice. Users can include all slices or select which slices to include in the final projection. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/afea159eccd62b4e59752aa3ccbf309999ecab529a250ff39ff54d89a8d39bcc.jpg) # Key features # Live Preview: Optimized for slide-based workflows Simplify the identification of regions of interest. Visualize your sample prior to acquisition using the virtual joystick to pan around the sample, and interactively adjust focus. Live Preview, featuring “click-to-center” functionality, continuously updates the image so you can easily navigate your sample to find the desired field of view. Whether you’re working on 96-well microplates, slides, or $3 5 ~ \\mathsf { m m }$ culture dishes, Live Preview helps you to quickly and easily focus on what’s important to your research. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/03491d33f1d86e69a3ccb6fb53561cb390dc4404cb89f7cd54c6cd3d1817b49d.jpg) # Digital Confocal: Restorative 2D on-the-fly deconvolution Significantly increase resolution and assay quality with Digital Confocal\* 2D on-the-fly deconvolution. The Digital Confocal Option restores light to its original point of origin, allowing you to decrease exposure time and improve statistical significance of your observations. Digital Confocal is seamlessly integrated into the ImageXpress Pico system’s fluorescent image acquisition workflow allowing you to capture images with higher signal-to-noise data for more precise segmentation and analysis. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d966f7905122e0019f66dd4adde5d373c1f2ca9a4439aa13ece0bfe09f75c148.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/7540f3fc41b51e0811694ac0deb17706d29725949086b835d51f5c538b7d127d.jpg) # Key features # Environmental control and time-lapse imaging Environmental control mimics the cell environment and enables you to run multiday studies, time-lapse, and live cell assays. With CellReporterXpress software, the ImageXpress Pico system provides visibility of environmental control settings for humidity, ${ \\mathsf { C O } } \_ { 2 }$ , and $\\mathsf { O } \_ { 2 }$ during acquisition to ensure that the system is running at peak performance during your assay. Monitor and measure changes in cell proliferation and cell phenotype over time with reliable time-lapse imaging and on-the-fly image analysis. The Plate View provides users a convenient at-a-glance Timecourse for cell proliferation view of their time-lapse results. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/3d4f3048730e5a3ed3227487ba408ffc9d307a1c60cb6be623d149ff2dc7f01b.jpg) # Monitor readings in real time. Acquired sensor data is linked to the experiment. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/87d98e25bbcaf1551aa1e3127bf3c0c6c47d11c1726d16a28d54749faf5ce10f.jpg) # Multi-wavelength cell scoring The ImageXpress Pico system with CellReporterXpress software features multi-wavelength cell scoring with up to four fluorescent stains. The preconfigured protocol is ideal for counting and logging measurements of cells in multiple wavelength experiments. Using a fluorescent marker for the nucleus and additional markers for the cytoplasm, each wavelength is analyzed and cells are assigned multiparametric phenotypic profiles. A simple interface minimizes setup efforts, and analysis settings can be configured once and saved for future use or customized to fit a specific experiment. Segmentation parameters are set for each wavelength and the analysis is run across the well, selected wells, the entire plate, or multiple plates. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/c01ca5d361f79de50613e1ac3c0a6f6a2bfe1f3c5d9afb40923646b1e37b7ff1.jpg) # Automated focus routines # Automated focus algorithms for a variety of applications including custom organ-on-a-chip plates The ImageXpress Pico system uses two robust autofocus mechanisms: Detect Surface, which is for hardware autofocus, and Find Best Plane, which is image-based autofocus. Hardware autofocus uses an LED beam to find reflective surfaces and is designed for speed. It works well for adherent samples in plates or chamber slides. When enabled, image-based autofocus searches a range for the best focus plane based on image contrast. It works well for slides with a coverslip or for samples in a plate that are not flat, such as suspension cells or spheroids. The ImageXpress Pico system with CellReporterXpress software provides reliable focusing across all of your labware to ensure high-quality imaging for a wide variety of sample types. # Detect surface algorithms ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/d84e943c7c49a727a49502aae376d1ae400e8c08b93cc5304d370d22860b1fa6.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1721fd848e99525311f7d8681287d73d50ab89eb649cf235bb5e35894ee98660.jpg) ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/92d1bcbddd0fb804dc3c27561a0132c583caf43bcc78fcaec388921863d813a2.jpg) # Plate Bottom Hardware autofocus detects the surface closest to the objective (that is, the plate bottom). It works well for adherent samples in plates or chamber slides when imaging at low magnification or if your labware has a thick bottom. This is the fastest hardware autofocus option. # Well Bottom Hardware autofocus detects the two surfaces closest to the objective (that is, the plate bottom and well bottom). The Well Bottom option is designed for samples in a liquid medium, such as well plates or chamber slides. This is the most commonly used hardware autofocus option. # Well Insert Hardware autofocus detects the three surfaces closest to the objective (that is, the plate bottom, well bottom, and well insert). The Well Insert option is designed for well inserts in well plates or any labware design that has a distinct third surface. # Find Best Plane Algorithms If the Detect Surface options do not provide satisfactory focus, select the Find Best Plane option to add image-based autofocus for the first channel within the selected search range. Adding image-based autofocus is useful for thicker samples, samples with variable best focus planes, or labware with variable thickness. The Find Best Plane algorithms center on the surface detected by the hardware autofocus and search within a specific range to find the optimal focal plane. Normal Search Range searches a range of $7 . 5 %$ above and $7 . 5 %$ below the labware bottom, and Wide Search Range searches a range of $20 %$ above and $20 %$ below the labware bottom. Unlike these two options, which search around the surface, the Superwide Search Range searches a range of $3 0 0 ~ { \\mu \\mathrm { m } }$ above that surface. # Anchor Focus Position For fastest screening speed, we have introduced the ability to select the Anchor Focus Position to save current focus position and disable the autofocus controls. The software uses the saved focus position for any ensuing snaps of preview images and for acquisition. This can dramatically speed the acquisition of plates at low magnification or when you are working with macroscopic samples like whole organisms or tissues. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/46ef5563e16ffaf473fe7b8bd7390cd4aa10d13bb331a39a5d54b7a4b4e61972.jpg) # Applications # From samples to results in minutes The CellReporterXpress software features over 25 preconfigured acquisition and analysis templates optimized to collect ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/f1bf09833f65d063ff10b211caf2b96bf920aac8a6e180c4758d96d672c3b4ce.jpg) # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. USA and Canada +1.800.635.5577 Taiwan/Hong Kong +886.2.2656.7585 United Kingdom +44.118.944.8000 Japan +81.3.6362.9109 Europe\* 00800.665.32860 South Korea +82.2.3471.9531 China +86.4008203586 India +91.73.8661.1198 \*Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland ## ImageXpress Micro Confocal ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/41a4779e7c8e7dbca6b757a6d01ab68aab4f0ebb06244e1f1bd140390fedd6aa.jpg) # ImageXpress Micro Confocal High-Content Imaging System The confocal solution for your complex biology # Deeper insights into complex biology Higher quality images, faster throughput and more powerful analysis The ImageXpress® Micro Confocal High-Content Imaging System provides improved quantification for live or fixed cell assays. This versatile imaging system features a unique confocal technology which allows you to explore more physiologically relevant, complex three dimensional models including spheroids, tissues, and whole organisms and to generate publication quality images at high throughput for samples in slides or one to 1536‑well microplates. # Key capabilities • Select a confocal geometry optimal for your assay and throughput needs • Achieve excellent image quality without sacrificing throughput via our unique optical path technology • Acquire statistically relevant data quickly with an advanced scientific CMOS detector, enabling $^ { > 3 }$ log dynamic range • Large field of view enables whole-well imaging • Expand your research capabilities with water immersion objectives, transmitted light, phase contrast optics, on-board liquid handling, and environmental control options # Confocal technology at the speed of widefield imaging • Capture an entire well of a 384‑well plate with a single image at $4 \\times$ magnification • Capture four wells of a 1536-well plate in a single image at $4 \\times$ magnification • Throughput of $> 1 6 0 1$ wells/day confocal, $> 2 0 0 \\mathsf { K }$ wells/day widefield # AgileOptix technology at the heart of the ImageXpress Micro Confocal system Software-selectable configurations right for your research The ImageXpress Micro Confocal system features AgileOptix™ Spinning Disk Technology. Our optical options make it easy to select and configure your system to ensure the best read for your assay. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/2b9a3130ed9d4a14669610d0e3bb0f6529e0767993955485a433415cd372251f.jpg) # Supports subcellular assays through whole organism assays • Widest selection $( > 2 5 )$ of objectives • Oil objectives with up to 1.4 NA • Air objectives with 0.05 to 0.95 NA • Water objectives with up to 1.2 NA # Select a spinning disk confocal geometry matched to your assay requirements. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1c835bd0955a33ce1616079ba2f49b74e512a5d2a8c25ec6cb8709f7dfebcd26.jpg) | | | | | | --- | --- | --- | --- | | Spinning disk geometry | 60 μm pinhole (single disk) | 60 μm dual disk with 50 μm slit | 60 μm dual disk with 42 μm pinhole | | High-sensitivity detection | | · | · | | Fast acquisition | | | | | >3 log dynamic range\* | | | . | | Widefield mode for flat biology | | | . | | Most confocal applications | | . | | | Highest resolution imaging | | | . | | High throughput applications | | | | \*Powered by our highly responsive sCMOS sensor and advanced solid state light source. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/e70ab4f97dd854a4c253124afb093b4e1eada29509eea6ce2172ec57d699218f.jpg) # eEhaxviopr maorne edffecytivoetluyumthroanbrehasvioer maosrpechefrhfoeditisvnferlotyumtomhcaoarnabcehrnacveielol rlwnmesospr ehdHeCrifoTfie1m1dc6sti fvereolynmtchsaincoernc e,Mmourceh irkelteuvmoarns,t hrebeyescauoulnsteasi,nmwuicth ik3ienDtfulamsokrs,athy3e7byme°cCcoanudtnsadei ,nl5sm%uiCcnhtOl2oikbneceftflouoarsmekosrpsal,tet3hx7ey°bCcioaonltdaoi5ng%y Complex, 3D cellular models yield more predictive, physiologically relevant results versus monocultures or other 2D cellular models. Explore the complexities of these models faster and gain better results even in samples grown in a thick extracellular matrices using the ImageXpress Micro Confocal isnygstpeotme.nIt aolfcfearnscfelerxtihbieldreaepnotepiftuyitioincngs ptotemnetie(atlFyBcoaS)nu.rcWesriptthehicnei rfd2iace4prnehteiuofstyiueicrnasrg.,caphsointnegnelte(idaFslsBpcaShan).nedrcWoeiitrndhtsihnuer2rea thalelebnegsetsi tmoadgevseflorpiynoSguormoaebsuscsahtya.l eGnegt emsotfroerdimenevsidegliohntptiihnetgSorbo3ombtDetuoscmthe lalolslfewnaigtchehsofwtopoert ilmldoeaenvnds ildikaesswaaytse: r immersion sopbhjerctoiivdeasstsoaiysm:prcovnet nsiugendalgrusopwhtioenrg4o tndimsaisezse ayutsn:tdilceiotpntwthianasuned ndgecarnedafsoincugsionpgt coan tahbe•esLrproahcteiatorionisgd fiaonrdbfeotcteurs iedndagtfaonrqteuhxaelp•tesyrLpiohomcrearmtnoitinoadgtni ontaonrdacffteoelclruh2s-ei4nadgdtfhoao kwienlletsiocsitocvaenrbtiemiemausgiendgevnenrayvsriwonegnllmeseonitaclactno3bn7ter°ioClm. # Clearer images and improved quacnotimtaptoivuneds:cretoepnoisnidgef,opra: -•f o cSupsheorobiadcs -•ofo mZcuesbroarfibsahbcyakangdrdodiuCn.ngdecseoigmoanpuantlos• Thick tissue samples • Homogenous no-wash assays • Organoids ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/49c8ffea8b8d2f1118725fc2dd4a770b892cc611a77549b78dd47e14c8a6cc9c.jpg) frCoellmstained for nuclei and actin growing in ae 3D gel. Projection image of seven planes acquired with a $4 0 \\times$ Plan Apo objective. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/bb5578a4d921eaf4fa98703f54ad63e8c40cfdae56056bc774d84bac448fe51e.jpg) pheroids in round bottom plates. Dose–dependent effects of selected compounds. Image montage of the 384 plate. Entire spheroid is captured with one mage, example segmentation of spheroid and 4-parametric curve fits for the number of live cells in spheroids. # Improve visualization and quantitation with 3D assay models Confocal capability improves image clarity and data quality Compared with widefield imaging, images of thick samples captured with confocal have reduced background and improved sharpness, resulting in improved image segmentation. Further improve signal and sharpness by using water immersion objectives to capture brighter images at lower exposure times. # Enhanced imaging of tissues and 3D matrices • Select specific cells of interest in 3D matrices such as neurons and stem cells • Reject high-background fluorescence in thick tissue samples • Acquire Z-stacks easily with 3D reconstruction capability # Clearer imaging of whole organisms • Large field of view enables imaging of an entire well of a 384‑well plate with a single image • Organisms remain in focus for the duration of the experiment • Perform sophisticated analysis of images and create time-lapse videos ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/deabe74d2c8690182df8285aaa73851e50ec9f7efaef68bd7895fe32b0940f5a.jpg) Rat brain section, stained for nuclei and neural outgrowths. Images taken with a $2 0 \\times$ Plan Fluor ELWD objective, confocal with $6 0 ~ \\mu \\mathrm { m }$ pinhole. Left Without segmentation and right with image segmentation with Neurite Outgrowth module. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/8cf19c932e81817f9e2275c9a5e1189a48d99a0a1535a6f4335c36bc44adbcda.jpg) # A complete solution for screening your most complex biological questions Delivers seamless workflows from image acquisition to data analysis Enjoy the benefits of a streamlined high-content screening (HCS) workflow in a fully integrated environment with our complete imaging solution for your most complex biological questions. # Acquire MetaXpress® High-Content Image Acquisition and Analysis Software powers our ImageXpress Micro Confocal system, giving you precise control over image acquisition and analysis, all within a unified interface. • Acquisition wizard for the entire workflow guides protocol setup for ease of use in multi dimensional imaging • Laser-based and software configurable image-based auto-focusing system ensures robust focus across a range of sample types • Acquisition of live cell images enables monitoring of cell growth, death, differentiation, and migration; viral or bacterial invasion, cancer metastasis, chemotaxis, drug toxicity, or translocation ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/31ec6f5d02be590dad464a0352cb9f57890cf6e9831f70a55cd457de7da92992.jpg) Live cell acquisition and analysis. HeLa cells expressing Cell Cycle Chromobody undergoing normal cell division while being imaged in confocal mode. In G1, the cells have a homogeneous fluorescence signal. During S phase, signal accumulates in the nucleus with formation of foci. In G2, the morphology returns to homogenous and the cell divides. White arrows indicates cell before cell division and yellow arrows indicates daughter cells after division event. ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/890df1bc894df88b0750d02ff87528afdb223f622f49ca5594ca3757683d26a7.jpg) # Analyze Avoid delays in image analysis and data processing using our MetaXpress software with application modules that allow you to quickly and easily analyze your data. • Plug-and-play application modules can be adapted to hundreds of image-based analysis workflows • Custom module editor empowers you to further tailor your image analysis routines for a perfect fit • Adaptive Background Correction™ adjusts image segmentation to the local intensity ranges and featur within and between cells for better quantitation • 2D projection algorithms include best focus, maximum and minimum, and sum projection for easy interpretation of 3D image data • 3D volumetric analysis evaluates volume, XYZ location, distance to neighboring objects, diameter, depth, various intensity measurements, texture, or number of objects • Save as cell-by-cell and/or image-by-image data AcuityXpress™ Informatics Software, data visualization, mining, and hit selection are ready to use upon system installation. # Store Regardless of the acquisition system used, images taken can be stored in the secure MDCStore™ Data Management Solution. • Accessible for sophisticated analysis by the MetaXpress® software • Data migration portal for integration with third-party imaging systems or analysis tools to external host databases or third‑party applications ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/831b01760668971dff96dad68baf0b8062ae40c1a45f9ef1829f120ad6b83519.jpg) Image Analysis MetaXpressand MetaXpress PowerCore Software ![](https://www.moleculardevices.com/sites/default/files/en/assets/brochures/dd/img/images/1a1a14bcc25fd284f902883ef037a4fe9fa931983386a2d1250b7dcd646ab1dc.jpg) Data Mining AcuityXpress Software # Specifications # System # AgileOptix optical path • High-speed laser autofocus with integrated image autofocus option • Linear encoded voice coil driven X, Y, and Z stages with better than 25 nm resolution • 4-position automated objective changer\* • 5-position software selectable dichroic filter wheel\* • 8-position software selectable emission filter wheel\* • Sample compatibility: slides and one to 1536‑well microplates, round or flat bottom, low to high profile \*User changeable • AgileOptix technology enables the ImageXpress Micro Confocal system to deliver the sensitivity and throughput needed for demanding applications by combining a powerful solid-state light engine, high-quantum efficiency 16-bit, $^ { > 4 }$ megapixel scientific CMOS sensor, and selectable confocal geometries • Large field of view ( $1 . 9 6 ~ \\mathsf { m m } ^ { 2 }$ at $1 0 \\mathsf { X } )$ imaging maximizes collection of publication quality images and statistically relevant data • $^ { \> 3 }$ log dynamic range is available in both widefield and confocal modes • Confocal can be purchased in one of the following three configurations: • Single-disk configuration with $6 0 \\mu \\mathrm { m }$ confocal pinhole and widefield modes • High-throughput dual disk configuration with $6 0 \\mu \\mathrm { m }$ confocal pinhole, unique and exclusive $5 0 ~ { \\mu \\mathrm { m } }$ slit confocal and widefield modes • High-resolution dual disk configuration with $6 0 \\mu \\mathrm { m }$ and $4 2 \\mu \\mathrm { m }$ confocal pinholes and widefield modes • Laser illumination or custom light sources available | | | | --- | --- | | Option | Feature | | Water Immersion Objectives | ·20X,40X,and 60X (up to 1.2 NA) ·Increase signal up to 4 times for brighter intensity at lower exposure times | | | | ·Increase in penetration depths dependent on sample | | ·Improve z-resolution and decrease optical aberrations ·Auto water replenishment enables screening or imaging across a plate | | Environmental Control | ·Multi-day, live cell time-lapse imaging | | · Provides appropriate atmospheric conditions (e.g. 5% or 10% CO2) | | ·Mimics physiological environment (30-40 °C ± 0.5 C) ·Controls humidity and minimizes evaporation (O0.5 μL/wel/hour for 96- or 384-well formats) | | ·High contrast imaging where unstained cels are easily viewed or separated from background (4X-60X) | | Phase Contrast | · Ideal for non-fluorescent histochemically stained samples | | ·Nikon 10OW Pillar Diascopic Iluminator with TE-C ELWD Condenser | | | | ·0.3 NA with 65 mm WD and PhL,Ph1,and Ph2 selectable phase rings ·Fluorophore-independent morphology visualization with fluorescent imaging overlay | | On-board Fluidics | ·Single-channel pipettor | | · Dispense volumes from 3 μL to 200 μL (±1 μL; ±5%) | | ·Compatible with 96- or 384-well format FLIPR System pipette tips | | ·Holds two plates for compound addition or media exchange | Note: all options, filters, and objectives are available at point of sale or as after market upgrades. Configurations shown herein do not encompass all configurations vailable. Contact your sales and support team today to identify the system configuration most suitable for your applications. # Contact Us # Regional Offices Phone: +1.800.635.5577 Web: [www.moleculardevices.com](http://www.moleculardevices.com/) Email: [info@moldev.com](mailto:info@moldev.com) Check our website for a current listing of worldwide distributors. 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