Dated: Nov 15, 2022
Publication Name: ACS Synthetic Biology
Citations : Robotic Construction and Screening of Lanthipeptide Variant Libraries in Escherichia coli
Lanthipeptides are a major class of ribosomally synthesized and post-translationally modified peptides (RiPPs) characterized by thioether cross-links called lanthionine (Lan) and methyllanthionine (MeLan). Previously, we developed a method to produce mature lanthipeptides in recombinant Escherichia coli, but manual steps hinder large-scale analogue screening. Here we devised an automated workflow for creating and screening variant libraries of haloduracin, a two-component class II lanthipeptide.
Contributors: Erpeng Guo, Lihao Fu, Xiaoting Fang, Wenhao Xie, Keyi Li, Zhiyu Zhang, Zhilai Hong, and Tong Si