Dated: Feb 22, 2023
Publication Name: Journal of Genetic Engineering and Biotechnology
Citations : Optimization of recombinant antibody production based on the vector design and the level of metabolites for generation of Ig- producing stable cell lines
The biopharmaceutical industry is significantly growing worldwide, and the Chinese hamster ovary (CHO) cells are used as a main expression host for the production of recombinant monoclonal antibodies. Various metabolic engineering approaches have been investigated to generate cell lines with improved metabolic characteristics for increasing longevity and mAb production. A novel cell culture method based on the 2-stage selection makes it possible to develop a stable cell line with high-quality mAb production.
Contributors: V. A. Toporova, V. V. Argentova, T. K. Aliev, A. A. Panina, D. A. Dolgikh & M. P. Kirpichnikov