Dated: Apr 26, 2022
Publication Name: Nature Catalysis
Citations : Efficient exploration of terpenoid biosynthetic gene clusters in filamentous fungi
Terpenoids, critical components of human medicine, are the largest family of natural products. Fungi are an important source of terpenoids, but many of the corresponding biosynthetic gene clusters (BGCs) are silent in laboratory conditions. Strategies such as homologous activation and heterologous expression were usually used to active a single cluster, making them low efficiency. Here we developed an automated and high-throughput (auto-HTP) biofoundry workflow using Aspergillus oryzae as a chassis that enables efficient genome mining, characterization of BGCs and identification of bioactive fungal terpenoids.
Contributors: Yujie Yuan, Shu Cheng, Guangkai Bian, Pan Yan, Zhengning Ma, Wen Dai, Rong Chen, Shuai Fu, Huiwen Huang, Haoming Chi, Yousheng Cai, Zixin Deng & Tiangang Liu