Dated: Dec 15, 2020
Publication Name: Molecular Devices
Citations : Cytotoxicity in Cells: Easy Determination Using Lonza ViaLight Plus and ToxiLight BioAssays on the SpectraMax L Microplate Luminometer
Bioluminescent cytotoxicity assays offer the user increased detection limits, speed, and accuracy, and have been well documented (Crouch et al., 1993). Healthy cells maintain a high constant ATP concentration in culture. During proliferation, the total ATP in the culture increases in line with cell number. Conversely, dying cells are unable to maintain their high ATP level, as synthesis becomes compromised, and so levels are seen to reduce. The level of ATP present within a culture is indicative of the number of viable cells present, and ATP assays have been shown to be one of the most predictive general cytotoxicity methods
Contributors: Claire Scholfield Ph.D, Tracy Simmons Ph.D, Susan Gill M.Sc., Lonza Walkerville, Inc.; and Cathy Olsen Ph.D, Molecular Device