Event Details

Implement high-throughput 3D image analysis for samples from subcellular structures to spheroids

  • Webinars
Implement high-throughput 3D image analysis - Labroots - Oksana - Luke
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Speakers

Oksana Sirenko, PhD Research Scientist, Molecular Devices

Steven Luke Applications Scientist, Molecular Devices

Abstract

3D assays have been gaining popularity as researchers look for models with improved physiological relevance. However, scaling up these assays can be challenging for creating a seamless workflow from image acquisition to analysis. In this webinar we present our latest technology advances for high-throughput imaging. Two types of assay examples are presented:

  • The development and optimization of a model system using human iPSC-derived hepatocytes to form spheroids for drug screening or assessing toxicity effects. The presentation will include experimental workflow, imaging techniques, 3D analysis and IC50 results for several compounds.
  • A two-dimensional cell culture in 384-well plates treated with mitotoxic compounds. Within each cell, mitochondria can be identified and characterized to generate measurements such as average mitochondrial count, total volume, distance from the nucleus, shape factor, and intensity.

Learn about:

  • Working with spheroids from seeding to treating to staining
  • Imaging over multiple Z-planes paired with the use of our integrated, user-friendly 3D analysis toolkit
  • Setting up analysis to provide data beyond intensity and number of objects, including morphology and volume of 3D structures