The course will provide theoretical introduction, practicalities to prepare samples and set up experiments, and basic and advanced data analysis in several modern methods for monitoring and quantifying molecular interactions:
- Biolayer interferometry (BLI)
- Surface plasmon resonance (SPR)
- Analytical ultracentrifugation (AUC)
- Isothermal titration calorimetry (ITC)
The workshop will focus on hands-on data analysis, using both tutorials with sample data and real data analysis (including one-on-one sessions with course tutors). The course will put emphasis on the complementarity of these different methods in the field of macromolecular biology and on the combined analysis of datasets coming from different methods.