Videos & Webinars

Hybridoma selection using HAT medium

Hybridoma selection after fusion of myelomas and spleen cells is a critical step in monoclonal antibody production. Often scientists use the HAT (hypoxanthine-aminopterin-thymidine) method to accomplish this task.

During the fusion process, three types of cells are present: (1) unfused myeloma cells that are deficient in an enzyme called HGPRT, (2) unfused spleen cells, and (3) fused hybridoma cells.

Please watch our tutorial video for more details.