Structural organization and functional analysis of compound responses in 3D human iPSC-derived cardiac tri-culture microtissues

In this video, Simon Lydford, Senior Application Scientist at Molecular Devices, presents his poster on why 3D cell models are becoming more common, and how the FLIPR Penta system can perform functional assays on such models, such as cardiac microtissues.

Recent publications show that tri-cellular co-culture microtissues of cardiomyocytes, endothelial cells and cardiac fibroblasts all derived from human iPSC enhance the maturation and functional activity of cells compared to 2D cardiomyocytes and thus more closely mimics actual heart physiology. The data presented here highlight the utility and biological relevance of using iPSC-derived cell types in 3D microtissues as a promising model for measuring compound effects on human cardiac tissues in high throughput format.