Tutorials & Videos

A Microfluidics-Based Single Cell Isolation Workflow Optimized for Efficiency, Viability, and Assurance of Monoclonality

Speaker: Steve Wiltgen, PhD, Molecular Devices

Sorting and isolating single cells is a crucial step in many applications including antibody drug discovery and cell line development. Conventional techniques for single cell isolation, such as limiting dilution and flow cytometry, are significantly restricted by process inefficiencies including low plating densities, low cell viabilities, and limited assurance of monoclonality. Microfluidics-based methods hold promise for improving upon such inefficiencies due to their ability to sort and isolate single cells in a low stress environment. In this webinar, we present how the microfluidics-based single-cell printing technology (the CloneSelect™ Single-Cell Printer™) significantly improves cell viability and deposit efficiencies while simultaneously providing strong assurance of monoclonality.

Come learn how you can:

  • Improve cell viability of CHO cells following single cell deposition into microplates
  • Reduce costs and timelines both upstream and downstream by improving clonal outgrowth efficiencies
  • Provide direct evidence of monoclonality in combination with a colony imager

Speaker Bio

Dr. Steve Wiltgen is the Product Manager for Bioproduction Development at Molecular Devices. He obtained his Bachelor's of Science degree in Mathematics and Biological Sciences from the University of Nebraska-Lincoln and his Ph.D. in Neurobiology and Behavior from the University of California, Irvine, where his research focus was developing a novel super-resolution imaging technique to look at ion channel function. Steve has been at Molecular Devices for 5 years, first serving as a Field Applications Scientist and now as Product Manager. 

Microfluidics Based Single Cell Isolation Workflow for Assurance of Monoclonality