SCIENTIFIC POSTER
Novel analysis of neural outgrowth in 3D human brain micro-tissues with application in compound screening
Brain micro-tissues reveal greater mechanistic insights as an industrial drug-screening platform
Brain micro-tissues show great promise in the study of neurological development and disease. However, they tend to be difficult to grow and can vary in shape, size, density, and opacity, thus rendering many unsuitable for high-throughput screening.
In this application note, we implemented high-content imaging of 3D brain micro-tissues to discover deeper insights and successfully develop an algorithm to analyze the neurites, nodes, and cell bodies. In addition, this innovative combination of high-content imaging with 3D human brain micro-tissues was applied to drug screening to reveal greater mechanistic insights as an industrial drug-screening platform.
Learn more about the systems behind the methods:
ImageXpress Micro Confocal system
High-content confocal imaging solution with proprietary spinning disk technology and water objective options
- Ideal for 3D organoid and spheroid imaging
- Proprietary AgileOptix™ spinning disk technology
- QuickID reduces image acquisition time and data storage requirements
- Water immersion objectives enhance resolution, sensitivity and throughput of complex
3D assays - Lasers and deep tissue confocal disk provide deeper penetration into 3D samples
- 3D volumetric analysis
MetaXpress software for ImageXpress high-content imaging systems
Multi-level analysis tools for a wide range of applications
- Meet high throughput requirements with a scalable, streamlined workflow
- Adapt your analysis tools to tackle your toughest problems, including 3D analysis
- Schedule automatic data transfer between third-party hardware sources and secure database
- Set up hundreds of routinely used HCS assays using MetaXpress software modules
- Export data to IN Carta software, leveraging intuitive, modern machine learning
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