Novel analysis of neural outgrowth in 3D human brain micro-tissues with application in compound screening

Brain micro-tissues reveal greater mechanistic insights as an industrial drug-screening platform 

Brain micro-tissues show great promise in the study of neurological development and disease. However, they tend to be difficult to grow and can vary in shape, size, density, and opacity, thus rendering many unsuitable for high-throughput screening.

In this application note, we implemented high-content imaging of 3D brain micro-tissues to discover deeper insights and successfully develop an algorithm to analyze the neurites, nodes, and cell bodies. In addition, this innovative combination of high-content imaging with 3D human brain micro-tissues was applied to drug screening to reveal greater mechanistic insights as an industrial drug-screening platform.