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Lung organoids as an assay model for in vitro assessment of toxicity effects by 3D high-content imaging and analysis

This poster describes the automated culture and a high-content imaging method that allows monitoring and characterization of growth and differentiation of lung organoids. Primary human lung epithelial cells were cultured in Matrigel supplemented with a mixture of growth factors that promote the formation of 3D structures recapitulating the morphological and functional characteristics of the airway. Organoids then self-organized into complex multi-lineage structures.

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