Image-based phenotypic profiling using Cell Painting in a 3D breast cancer spheroid model
Most potential oncology drugs fail the drug development pipeline, despite having promising data for their efficacy in vitro.
Here we demonstrate a screen using patient-derived 3D spheroids (tumoroids) where—in addition to readouts on cell viability—we adapted the Cell Painting assay for the 3D tumor model. Tumoroids were formed from primary cells isolated from a patient-derived tumor explant that represents metaplastic breast cancer with a triple-negative breast cancer. Results demonstrate the feasibility of using the Cell Painting assay on 3D cell models.
Learn more about the systems behind the methods:
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